TRANSBIO. Del laboratorio a la aplicación final.

BioTRANSformation of by-products from fruit and vegetable processing industry into valuable

BIOproducts

A European Project supported within the Seventh Framework Programme for Research and Technological Development

SSF FROM LAB TO FINAL APPLICATIONCaroline Hennigs, Naturstoff-Technik GmbH

03.11.2015, Final Conference TRANSBIO, Vitoria-Gasteiz (Spain)

Introduction


Where is NST

NST locations

• Dellstedt• Bremerhaven• Osterholz-Scharmbeck

Germany

Introduction


Who is NST● founded 01.10.1979 in Ritterhude● take over 01.07.2008 from Mr. Holmer Wöhlk● Opening of an R&D department in Bremerhaven in April 2009● contract manufacturing in

– Drying (Fluid Bed Dryer)– Agglomeration– Spraygranulation– Encapsulation / Coating– Mixing– Milling– Sieving– Process development (Bremerhaven)

● 2 employees at 01.07.2008 / currently 7 employees + temporary staff● production area 1100 qm● Plants: - WSG 5 / 60 / 120 / 500

- continuous belt dryer/cooler (1-2 t/h)- Solid State Fermentation plant (solid state fermentation in Dellstedt)

• Possibility for tests in technical scale in a good, fast and cheap way.

NST R&D department www.bio-nord.de

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Introduction

General Information


Solid-state fermentation is used for the production of

microbial products such as

• Feed

• Fuel

• Food

• Industrial chemicals

• Pharmaceutical products

Solid state fermentation at lab scale


SSF at lab scale: Koji production process

. Washing

Soaking

Rice steaming

Inoculation

Koji cultivation

Raw productsoaking

steaming

Inoculation

cultivation

Solid state fermentation at lab scale


Koji- Rice

Koji Soft drink

Vinegar

Baby mush

Application SSF products


Probiotic grain drink

Koji Rice wine (Sake)

Animal food (Agricolia)

Sweet white misoSojasoße

Black garlic

Medicine (immunsystem)

EggsAmazake

Koji production


SSF = Solid State Fermenter

Tray fermentation systemKoji made by hand since 2000years

Lab- scale


A better aeration is possible by bioreactor upscale

Try- bioreactor 0.5 – 1.0 kg

Fermenter prototype

Tray fermentation process


Reactor funktion (Lab scale)

•Chamber is incubated in a temperature-controlled room

•Water saturated air is added from the top.

•The trays fit inside the reactor and conditioned air is forced in at

the lower left portion of the reactor.

•The reactor is equipped with thermocouples, flow meter, oxygen

sensor and relative humidity sensor.

.


Listing of Substrates, Microorganisms and Enzymes produced

Advantages SSF


Advantages compared to submerged fermentation (General)

• Low production cost due to

• saving of water and energy

• less waste effluent problem

• Improved product stability due to lower dilution in the medium• Various applications of fermentation products

FoodFeedPharmaceuticalsIndustrial detergents

Holistic utilisation of the product = increased sustainability

Up- scale


Washing and soaking of rice grains

Rice grain steaming

Cool down and Inocculation with spores

Scale up to SSF at technical scale (600kg)

Up- scale


Scale up to SSF at technical scale (600kg)

SensorSpinning plateStirrer

Raw product inlet

Air cooling unit

Comparison Lab/ Technical scale

=> Koji rice ferment’s enzyme activity is extremely high=> Activity was even significantly increased by scale‐up

Lab scale (50 kg) Technical scale (600 kg)

Yield 108 – 110% 113 – 115%

Protease activity [U/mg] 106 1785

Lipase activity [U/mg] 30 800

Amilase [U/mg] 0,02 0,17

Up- scale


Up- scale


Scale up to SSF at industrial scale (1.200kg) By numbering up


Thank you very much for your attention!

Caroline HennigsNaturstoff-Technik GmbH

Marie-Curie Str. 1127711 Osterholz-Scharmbeck

Germany

TRANSBIO. Del laboratorio a la aplicación final.

Food

Transcript of TRANSBIO. Del laboratorio a la aplicación final.