BIO -NANOTECNOLOGÍA APLICADA A LA REGENERACIÓN ÓSEA ...
Transcript of BIO -NANOTECNOLOGÍA APLICADA A LA REGENERACIÓN ÓSEA ...
UNIVERSIDAD DE GRANADA
Facultad de Odontologiacutea
Departamento de Estomatologiacutea
BIO-NANOTECNOLOGIacuteA APLICADA A LA
REGENERACIOacuteN OacuteSEA MEDIANTE EL
TRANSPORTE DE BIOMOLEacuteCULAS USANDO
NANOPARTIacuteCULAS POLIMEacuteRICAS ESTUDIO IN
VITRO
Inmaculada Ortega Oller
TESIS DOCTORAL
Programa de Doctorado en Medicina Cliacutenica y Salud Puacuteblica
Editor Universidad de Granada Tesis Doctorales Autor Inmaculada Ortega Oller ISBN 978-84-1306-879-4
URI httphdlhandlenet1048168568
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BIO-NANOTECNOLOGIacuteA APLICADA A LA
REGENERACIOacuteN OacuteSEA MEDIANTE EL
TRANSPORTE DE BIOMOLEacuteCULAS USANDO
NANOPARTIacuteCULAS POLIMEacuteRICAS ESTUDIO
IN VITRO
por
Inmaculada Ortega Oller
Licenciada en Odontologiacutea
Directores de la Tesis
Dr D Joseacute Manuel Peula
Prof Titular de Fiacutesica Aplicada
Dr D Francisco OacuteValle Ravassa
Prof Catedraacutetico de Anatomiacutea Patoloacutegica
Dr D Pablo Galindo Moreno
Prof Catedraacutetico de Estomatologiacutea
4
A mi familia
y directores y en especial
a mi PADRE
Juan Ortega Navarro
5
Agradecimientos
Despueacutes de un apasionado y largo periacuteodo de elaboracioacuten de esta tesis doctoral hoy es el diacutea
escribo este apartado de agradecimientos para finalizar un arduo trabajo Ha sido un periacuteodo de
aprendizaje intenso no solo en el campo cientiacutefico sino tambieacuten a nivel personal que ha supuesto
un gran impacto en miacute Por tal motivo me gustariacutea agradecer a todas aquellas personas que me
han ayudado y brindado su apoyado durante este proceso
En primer lugar quiero agradecer a mis tutores
Don Jose Manuel Peula Garciacutea Profesor Titular de Fiacutesica Aplicada quien con sus
conocimientos y apoyo me guioacute a traveacutes de cada una de las etapas de este proyecto para alcanzar
los resultados que buscaba Gracias por su paciencia calma y tranquilidad para explicar
detenidamente a una odontoacuteloga todos y cada uno de los conceptos aprendidos e interiorizados
Todo mi agradecimiento por su tiempo dedicacioacuten y por haberme acogido de la manera que lo
hizo daacutendome todo su carintildeo apoyo y compresioacuten
Don Pablo Galindo Moreno Profesor Catedraacutetico de Estomatologiacutea
Un trabajo de investigacioacuten es siempre fruto de ideas proyectos y esfuerzos previos que
corresponden a otras personas y que te escogen a ti para saber llevarlas a cabo En este caso mi
maacutes sincero agradecimiento a usted con cuyo trabajo estareacute siempre en deuda y con quien he
compartido proyectos e ilusiones durante todos estos antildeos Gracias por su amabilidad para
facilitarme su tiempo y sus ideas Ha sido una fuente de paz en tiempos muy duros
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Don Francisco OacuteValle Ravassa Profesor Catedraacutetico de Anatomiacutea Patoloacutegica por su
orientacioacuten atencioacuten a mis consultas y por sus valiosas sugerencias en momentos de duda asiacute
como por su completa disponibilidad siempre que lo he necesitado
Tambien quiero agradecer a Mis Iberica SL y a la Consejeriacutea de Economiacutea Innovacioacuten
Educacioacuten Ciencia y Empleo de la Junta de Andaluciacutea por brindarme todos los recursos y
herramientas que fueron necesarios para llevar a cabo el proceso de investigacioacuten No hubiese
podido arribar a estos resultados sin su incondicional ayuda
Un trabajo de investigacioacuten es tambieacuten fruto del reconocimiento y del apoyo vital que nos
ofrecen las personas que nos estiman sin el cual no tendriacuteamos la fuerza y energiacutea que nos anima
a crecer como personas y como profesionales este es el caso del Dr Don Miguel Padial y de las
Dras Dontildea Azahara Rata-Aguilar Dontildea Ana Jodar y DontildeaTeresa Del Castillo Gracias por
vuestra cercana visioacuten de todo Tambien queriacutea dedicar unas palabras a un buen amigo Javier
Vidao quien con sus conocimientos informaacuteticos me ha ayudado con la elaboracioacuten de este
documento de tesis Gracias
Quiero agradecer tambieacuten a todos mis compantildeeros y amigos por hacer feliz mi dia a dia y a mi
familia por apoyarme auacuten cuando mis aacutenimos decaiacutean A mis hermanos Juan Javier y Dorothy y
a mi madre que siempre estuvieron ahiacute para darme palabras de apoyo y un abrazo reconfortante
para renovar energiacuteas
Gracias a mi pareja por su paciencia comprensioacuten y solidaridad con este proyecto por el
tiempo que me ha concedido un tiempo robado al disfrute conjunto que ha respetado y valorado
Te agradezco la esperanza que me has brindado en los momentos y situaciones mas tormentosas
de mi vida
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Por uacuteltimo dedico con todo mi corazoacuten mi tesis doctoral a mi PADRE a quien perdimos
recientemente pues sin eacutel no habriacutea logrado nada de esto gracias por haberme forjado como la
persona que hoy soy
Muchos de mis logros se los debo a eacutel quien me educoacute con firmeza pero a la vez sabiendo
darme la libertad suficiente para permitirme evolucionar por mi misma como persona
motivandome constantemente a alcanzar mis metas Fue mi referente en la vida y quien me dioacute el
uacuteltimo y maacutes grande de los aprendizajes dejando en miacute un gran espiacuteritu de lucha sacrificio
esfuerzo y sabiduriacutea ante la maacutes difiacutecil situacioacuten planteable Por todo ello este trabajo te lo dedico
a ti alliacute donde esteacutes espero que seas feliz y puedas disfrutar de los logros conseguidos aquiacute
A todos muchas gracias
8
RESUMEN
REGENERACIOacuteN OacuteSEA A PARTIR DE NANOMICROPARTICULAS DE PLGA
CARGADAS DE BMP-2
El aacutecido poli-laacutectico-co-glicoacutelico (PLGA) es uno de los poliacutemeros sinteacuteticos maacutes ampliamente
utilizados para el desarrollo de sistemas de administracioacuten de faacutermacos y biomoleacuteculas
terapeacuteuticas asi como componente principal en aplicaciones de ingenieriacutea de tejidos Sus
propiedades y versatilidad le permiten ser un poliacutemero de referencia en la fabricacioacuten de
nanopartiacuteculas y micropartiacuteculas para encapsular y liberar una amplia variedad de moleacuteculas
hidrofoacutebicas e hidrofiacutelicas Ademaacutes sus propiedades de biodegradabilidad y biocompatibilidad
hacen del mismo un candidato idoacuteneo para encapsular biomoleacuteculas como proteiacutenas o aacutecidos
nucleicos permitiendo su liberacioacuten de forma controlada
Este trabajo se centra en el uso de nanopartiacuteculas (NP) de PLGA como un sistema de entrega
de uno de los factores de crecimiento maacutes comuacutenmente utilizados en la ingenieriacutea del tejido oacuteseo
la proteiacutena morfogeneacutetica oacutesea 2 (BMP2) Por lo tanto examinamos todos los requisitos
necesarios para alcanzar una correcta encapsulacioacuten y una liberacioacuten controlada y sostenida de
BMP2 utilizando partiacuteculas de PLGA como componente principal discutiendo todos los
problemas y soluciones que hemos encontrado para el desarrollo adecuado de este sistema con un
gran potencial en el proceso de diferenciacioacuten celular y proliferacioacuten bajo el punto de vista de la
regeneracioacuten oacutesea
Hemos desarrollado y optimizando dos meacutetodos de formulacioacuten diferentes para obtener NP de
PLGA cargadas con una proteiacutena modelo con actividad enzimaacutetica como la lisozima que posee
caracteriacutesticas similares a la BMP2 Estas formulaciones se basan en una teacutecnica de doble
emulsioacuten con evaporacioacuten de solvente (agua aceite agua WOW) Se diferencian
principalmente en la fase en la que se agrega el surfactante (Pluronicreg F68) agua (W-F68) o
9
aceite (O-F68) Este surfactante polimeacuterico no ioacutenico puede modular una serie de propiedades del
nanosistema transportador en el que se integra reduciendo el tamantildeo de las NPs incrementando
su estabilidad coloidal y facilitando la proteccioacuten de la biomoleacutecula encapsulada Ademaacutes gracias
a su disposicioacuten superficial y la hidrofilidad de sus colas polares se reduce la interaccioacuten con el
sistema fagociacutetico mononuclear con una mejora de la biodistribucioacuten al aumentar su tiempo de
circulacioacuten despueacutes de una administracioacuten intravenosa en un organismo vivo
Analizamos las propiedades coloidales de estos sistemas usando diferentes teacutecnicas
experimentales (morfologiacutea por SEM y STEM tamantildeo hidrodinaacutemico por DLS y NTA
movilidad electroforeacutetica estabilidad temporal en diferentes medios) asiacute como la encapsulacioacuten
patroacuten de liberacioacuten y bioactividad de la lisozima Asimismo realizamos una caracterizacioacuten
interfacial de la interaccioacuten surfactante-proteiacutena en la primera emulsioacuten agua-aceite para cada
procedimiento de formulacioacuten mediante el anaacutelisis de la tensioacuten superficial y la elasticidad
Finalmente examinamos la captacioacuten celular por ceacutelulas estromales mesenquimaacuteticas humanas y
la citotoxicidad para ambos nanosistemas
Mediante las dos formulaciones O-F68 y W-F68 se obtienen NPs soacutelidas de morfologiacutea
esfeacuterica si bien en un caso el sistema presenta monodispersidad con diaacutemetros alrededor de 120
nm (O-F68) en el otro se obtiene un nanosistema polidisperso con diaacutemetros de partiacutecula
comprendidos entre 100 y 500 nm (W-F68) Como resultado maacutes relevante observamos que la
eficacia de encapsulacioacuten la liberacioacuten y la bioactividad de la lisozima se han mantenido mejor
con el meacutetodo de formulacioacuten W-F68 En este caso dada la heterogeneidad de tamantildeos se podriacutea
hablar de un prometedor sistema multimodal para encapsular proteiacutenas con una fuerte actividad
bioloacutegica que permita una ldquoentrega dualrdquo a nivel extra- e intracelular facilitando la actividad
proteica en la superficie celular y en el citoplasma
Tras desarrollar y optimizar el meacutetodo de siacutentesis para las NPs de PLGA cargadas de lisozima
tratamos de adaptar la formulacioacuten para conseguir la encapsulacioacuten de la proteiacutena terapeacuteutica
BMP-2 Asiacute basaacutendonos en los resultados obtenidos con la lisozima se ha optado por usar el
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procedimento de siacutentesis W-F68 para favorecer la proteccioacuten de las moleacuteculas proteicas y su
actividad bioloacutegica Con esta formulacioacuten se han obtenido con buena reproducibilidad NPs
esfeacutericas con el tamantildeo multimodal referido anteriormente entre 100 y 500 nm que posibilitaraacuten
el suministro extra- e intracelular Ademaacutes de NPs con BMP2 encapsulada obtenemos un
nanosistema en el que la BMP2 no estaacute encapsulada sino co-adsorbida superficialmente junto a
una proteiacutena estabilizadora como la albuacutemina de suero bovino De nuevo se lleva a cabo una
completa caracterizacioacuten fisico-quiacutemica y bioloacutegica de ambos sistemas de NPs analizando las
propiedades indicadas previamente esto es morfologiacutea y tamantildeo carga superficial estabilidad
coloidal y temporal encapsulacioacuten y patroacuten de liberacioacuten Es conocido que la cineacutetica de
liberacioacuten en los sistemas polimeacutericos basados en PLGA dependen en gran medida de la
degradacioacuten hidroliacutetica del poliacutemero Sin embargo la liberacioacuten a tiempos cortos estaacute influenciada
por otros procesos fiacutesicos y es crucial evitar una descarga inicial excesiva sobre todo si se quiere
optimizar la aplicacioacuten de esta nanotecnologiacutea en procesos de regeneracioacuten oacutesea muy importantes
en odontologiacutea En consecuencia hemos incidido en el anaacutelisis del patroacuten de liberacioacuten de la
BMP2 a tiempos cortos utilizando diferentes teacutecnicas y comparando el comportamiento de los dos
sistemas de NPs con la proteiacutena encapsulada o adsorbida superficialmente
Finalmente se ha analizado la actividad bioloacutegica de las NPs cargadas con BMP2 mediante
estudios in vitro de proliferacioacuten celular migracioacuten y diferenciacioacuten osteogeacutenica usando para ello
ceacutelulas estromales mesenquimales obtenidas a partir de hueso alveolar humano (ABSC) En base
a todo esto se puede confirmar que las NPs con BMP2 encapsuladas presentan un patroacuten de
liberacioacuten adecuado a corto plazo manteniendo un suministro proteico sostenido y una actividad
bioloacutegica adecuada para dosis iniciales de BMP2 muy reducidas
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SUMMARY
BONE REGENERATION FROM PLGA NANOMICROPARTICLES LOADED
WITH BMP-2
Poly-lactic-co-glycolic acid (PLGA) is one of the most widely used synthetic polymers for the
development of drug delivery systems and therapeutic biomolecules and as a component of tissue
engineering applications Its properties and versatility allow it to be a reference polymer in the
manufacture of nanoparticles and microparticles to encapsulate and release a wide variety of
hydrophobic and hydrophilic molecules Furthermore its biodegradability and biocompatibility
properties make it an ideal candidate for encapsulating biomolecules such as proteins or nucleic
acids that can be released in a controlled manner This work focuses on the use of PLGA
nanoparticles (NP) as a delivery system for one of the most commonly used growth factors in
bone tissue engineering bone morphogenetic protein 2 (BMP2) Therefore we examine all the
necessary requirements to achieve a correct encapsulation and a controlled and sustained release
of BMP2 using PLGA particles as the main component discussing all the problems and solutions
that we have found for the proper development of this system with great potential in the process
of cell differentiation and proliferation from the point of view of bone regeneration We have
developed and optimized two different formulation methods to obtain PLGA NP loaded with a
model protein with enzymatic activity such as lysozyme with similar characteristics to BMP2
These formulations are based on a double emulsion technique with solvent evaporation
(wateroilwater WO W) They differ mainly in the phase in which the surfactant (Pluronicreg
F68) is added water (W-F68) or oil (O-F68) This non-ionic polymeric surfactant can modulate
a series of properties of the transporter nanosystem in which it is integrated reducing the size of
the NPs increasing their colloidal stability and facilitating the protection of the encapsulated
biomolecule Furthermore thanks to its superficial arrangement and the hydrophilicity of its polar
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tails interaction with the mononuclear phagocytic system is reduced with an improvement in
biodistribution by increasing its circulation time after intravenous administration in a living
organism The colloidal properties of these systems have been analyzed using different
experimental techniques (morphology by SEM and STEM hydrodynamic size by DLS and NTA
electrophoretic mobility temporal stability in different media as well as the encapsulation
release pattern and bioactivity of the lysozyme Likewise an interfacial characterization of the
surfactant-protein interaction was carried out in the first water-oil emulsion for each formulation
procedure by analyzing the surface tension and elasticity Finally we analyzed the cellular uptake
by human mesenchymal stromal cells and cytotoxicity for both nanosystems Through the two
formulations O-F68 and W-F68 solid NPs of spherical morphology are obtained although in
one case the system presents monodispersity with diameters around 120 nm (O-F68) while in
the other a Polydisperse nanosystem with particle diameters between 100 and 500 nm (W-F68)
As a more relevant result we observed that the encapsulation efficiency the release and the
bioactivity of lysozyme have been better maintained with the W-F68 formulation method In this
case given the heterogeneity of sizes one could speak of a promising multimodal system to
encapsulate proteins with strong biological activity that allows a dual delivery at the extra- and
intracellular level facilitating protein activity on the cell surface and in the cytoplasm After
developing and optimizing the synthesis method for lysozyme-loaded PLGA NPs we tried to
adapt the formulation to achieve encapsulation of the therapeutic protein BMP-2 Thus based on
the results obtained with lysozyme it was decided to use the W-F68 synthesis procedure to favor
the protection of protein molecules and their biological activity With this formulation spherical
NPs with the aforementioned multimodal size between 100 and 500 nm have been obtained with
good reproducibility which would allow extra- and intracellular delivery In addition to NPs with
encapsulated BMP2 a nanosystem has been obtained in which BMP2 is not encapsulated but is
superficially co-adsorbed with a stabilizing protein such as bovine serum albumin Again a
complete physico-chemical and biological characterization of both NPs systems is carried out
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analyzing the previously indicated properties that is morphology and size surface charge
colloidal and temporal stability encapsulation and release pattern
It is known that release kinetics in PLGA-based polymer systems are highly dependent on the
hydrolytic degradation of the polymer However the short-time release is influenced by other
physical processes and it is crucial to avoid an excessive initial discharge especially if the
application of this nanotechnology is to be optimized in very important bone regeneration
processes in dentistry Consequently we have focused on the analysis of the release pattern of
BMP2 at short times using different techniques and comparing the behavior of the two NPs
systems with the encapsulated or superficially adsorbed protein Finally the biological activity of
NPs loaded with BMP2 has been analyzed by in vitro studies of cell proliferation migration and
osteogenic differentiation using mesenchymal stromal cells obtained from human alveolar bone
(ABSC) Based on all this it can be confirmed that NPs with encapsulated BMP2 present an
adequate release pattern in the short term maintaining a sustained protein supply and adequate
biological activity for very low initial doses of BMP2
14
LISTA DE PUBLICACIONES
1 Ortega-Oller I Padial-Molina M Galindo-Moreno P OrsquoValle F Joacutedar-Reyes A B
Peula-Garciacutea J M Bone Regeneration from PLGA Micro-Nanoparticles Biomed Res
Int 2015 vol 2015 1ndash18 doi1011552015415289 IF Q2 Rank 82161 JIFpercentil
494 Nordm de citas 33
2 Ortega-Oller I del Castillo-Santaella T Padial-Molina M Galindo-Moreno P Joacutedar-
Reyes A B Peula-Garciacutea J M Dual delivery nanosystem for biomolecules
Formulation characterization and in vitro release Colloids Surfaces B Biointerfaces
2017 159 586ndash595doi 101016jcolsurfb201708027 IF Q1 Rank1272 JIF
percentil 826 Nordmcitas 5
3 del Castillo-Santaella T Ortega-Oller I Padial-Molina M OrsquoValle F Galindo-
Moreno P Joacutedar-Reyes A B Peula-Garciacutea J M Formulation Colloidal
Characterization and In Vitro Biological Effect of BMP-2 Loaded PLGA Nanoparticles
for Bone Regeneration Pharmaceutics 2019 11(8) 388
doi103390pharmaceutics11080388 IF Q1 Rank26267 JIFpercentil 905 Nordmcitas
3
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Iacutendice
0 GLOSARIO (lista de abreviaturas) helliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphellip 19
1 INTRODUCCIOacuteN 23
11 BMPS ACCIOacuteN Y REGULACIOacuteN 24
111 Uso cliacutenico de la BMP-2 27
12 PARTIacuteCULAS COLOIDALES POLIMEacuteRICAS PARA ENCAPSULAR MOLEacuteCULAS HIDROFIacuteLICAS 30
121Meacutetodos de siacutentesis 33
123 Tamantildeo y morfologiacutea de las partiacuteculas 35
13AGENTES ESTABILIZADORES 39
131 Estabilidad coloidal 39
132 Eficacia de encapsulacioacuten y bioactividad 42
14 PATROacuteN DE LIBERACIOacuteN 44
15 VECTORIZACIOacuteN ENTREGA DIRIGIDA 52
16 INGENIERIacuteA TISULAR SOPORTES 3D O ldquoSCAFFOLDSrdquo 53
2 HIPOacuteTESIS 55
3 OBJETIVOS 56
31 OBJETIVO PRINCIPAL 56
32 OBJETIVOS SECUNDARIOS 56
4 NANOSISTEMA DE ENTREGA DOBLE PARA BIOMOLEacuteCULAS FORMULACIOacuteN CARACTERIZACIOacuteN
Y LIBERACIOacuteN IN VITRO 58
41 ANTECEDENTES 58
42 MATERIALES Y MEacuteTODOS 60
421Formulacioacuten de las nanoparticulas 60
422 Limpieza y almacenamiento 61
423 Caracterizacioacuten de las nanoparticulas 62
424 Estabilidad coloidal y temporal en biologiacutea media 63
425 Actividad bioloacutegica e interacciones 64
43 RESULTADOS Y DISCUSIOacuteN 65
17
431 Formulacioacuten de las nanoparticulas 65
432 Caracterizacioacuten de las Nanopartiacuteculas 69
433 Actividad bioloacutegica e interacciones 84
5 FORMULACIOacuteN CARACTERIZACIOacuteN COLOIDAL Y EFECTO BIOLOacuteGICO IN VITRO DE
NANOPARTIacuteCULAS DE PLGA CARGADAS CON BMP-2 PARA LA REGENERACIOacuteN OacuteSEA 96
51 ANTECEDENTES 96
52 MATERIALES Y MEacuteTODOS 98
521Siacutentesis de nanoparticulas 98
522 Caracterizacioacuten de nanopartiacuteculas morfologiacutea tamantildeo concentracioacuten y movilidad electrocineacutetica
101
523 Estabilidad coloidal y temporal en medios bioloacutegicos 101
524 Interacciones celulares 102
53 RESULTADOS Y DISCUSIOacuteN 105
531Formulacioacuten de nanoparticulas 105
532Caracterizacioacuten de nanopartiacuteculas 109
533Actividad bioloacutegica e interacciones 118
6 CONCLUSIONES 125
7 CONFLICTO DE INTERESES 127
8 RECURSOS ECONOacuteMICOS 127
9 BIBLIOGRAFIacuteA 128
10 ANEXO MATERIAL SUPLEMENTARIO 154
- Enlace a videos
11 ANEXO DE PUBLICACIONES 156
-Artiacuteculo 1 Bone regeneration from PLGA Micro-Nanoparticles
-Artiacuteculo 2 Dual delivery nanosystem for biomolecules Formulation characterization and
in vitro release
18
-Artiacuteculo 3 Formulation Colloidal Characterization and In Vitro Biological Effect of
BMP-2 Loaded PLGA Nanoparticles for Bone Regeneration
12 ANEXO DE ORIGINALIDAD 158
19
LISTA DE ABREVIACIONES
NP Nanopartiacuteculas
MP Micropartiacuteculas
MSC Ceacutelulas mesenquimales
BMP Proteiacutena morfogeneacutetica oacutesea
Rh BMP Proteiacutena morfogeneacutetica oacutesea recombinante
BMP I y II Proteiacutena morfogeneacutetica oacutesea receptora I y II
GF Factor de crecimiento
PDGF Factor de crecimiento derivado de plaquetas
FGF Factor de crecimiento de fibroblastos
IGF Factor de crecimiento de insulina
TGF- Factor de crecimiento transformante
RUNX2 Factor de transcripcioacuten
MPS Sistema fagociacutetico mononuclear
hMSC Ceacutelulas mesenquimales humanas
ABSC Ceacutelulas estromales mesenquimales oacutesea
OSX Osterix
LMP Proteiacutena de mineralizacioacuten de dominio Lim
SEM Microscopia electroacutenica de barrido
STEM Microscopia electroacutenica de transmisioacuten de barrido
PLA Aacutecido poli-lactico
PLGA Aacutecido poli-lactico co-glicolico
LYS Lisozima
LYSF Lisozima final (encapsulada)
20
F68 Pluronic (W-F68= en agua) (O-F68= en aceite)
WOW Doble emulsioacuten de agua en aceite
AP Fase acuosa
OP Fase orgaacutenica
SA Albuacutemina seacuterica
BSA Albuacutemina seacuterica bovina
HSA Albuacutemina seacuterica humana
PVA Alcohol poliviniacutelico
PBS Tampoacuten fosfato salino
PB Tampoacuten fosfato
FBS Suero fetal bovino
PEO Oacutexido de polietileno
DCM Diclorometano
EA Acetato de etilo
FITC Isotiocianato de fluoresceiacutena
DPPC Dipalmitoil-fosfatidilcolina
PGE Polietilenglicol
DC Aacutecido dexosicoacutelico
BCA Aacutecido bicinconiacutenico
DTT Ditiotreitol
SDS Duodecil sulfato de sodio
ALP Fosfatasa alcalina
GAPDH Gliceraldehiacutedo-3-fosfato deshidrogenasa
SDS Gel duodecilsulfato de sodio
PI3K Fosfoinositida 3-quinasa
ALP Fosfatasa alcalina
21
SDS-PAGE Electroforesis en gel poliacrilamida con duodecilsulfato soacutedico
PDI Iacutendice de polidispersidad
DLS Dispersioacuten de luz dinaacutemica
EE Eficacia de encapsulacioacuten
SRB Absorbancia de sulforamida
DL Carga del faacutermaco
FDA Administracioacuten de medicamentos y alimentos
RMN Resonancia magneacutetica nuclear
NTA Anaacutelisis de seguimiento de nanopartiacuteculas
NLS Sentildeales de localizacioacuten nuclear
DMEM Medio Eagle modificado con dulbecco
23
1 INTRODUCCIOacuteN
La regeneracioacuten oacutesea es uno de los principales desafiacuteos a los que nos enfrentamos en la cliacutenica
diariamente Inmediatamente despueacutes de la extraccioacuten de un diente los procesos bioloacutegicos
normales remodelan el hueso alveolar limitando en algunos casos la posibilidad de una futura
colocacioacuten de implante En los uacuteltimos antildeos han sido estudiadas diferentes estrategias para llevar
a cabo la preservacioacuten de ese hueso Otras afecciones como el traumatismo la cirugiacutea de
reseccioacuten tumoral o las deformidades congeacutenitas requieren requisitos teacutecnicos y bioloacutegicos auacuten
mayores para generar la estructura oacutesea necesaria para la rehabilitacioacuten oclusal del paciente Para
superar estas limitaciones anatoacutemicas en teacuterminos de volumen oacuteseo existen diferentes enfoques
para mejorar la osteointegracioacuten del implante o para aumentar la anatomiacutea del hueso donde se
colocaraacute el futuro implante (M Padial-Molina P Galindo-Moreno 2009) (Al-Nawas and
Schiegnitz 2014) El injerto oacuteseo autoacutegeno todaviacutea se considera el ldquogold estaacutendarrdquo debido a sus
propiedades osteogeacutenicas osteoconductivas y osteoinductivas (Katranji Fotek and Wang 2008)
(Misch 1987) Sin embargo tambieacuten presenta varias limitaciones incluida la necesidad de una
segunda cirugiacutea disponibilidad limitada y morbilidad en el aacuterea donante (Myeroff and
Archdeacon 2011) Por lo tanto otros biomateriales como injertos alogeacutenicos e injertos
xenogeacutenicos con osteoconductividad y capacidades osteoinductivas (Avila et al 2010) (Froum
et al 2006) fueron propuestos (Galindo-Moreno et al 2007) (Galindo-Moreno et al 2011) asiacute
como biomateriales aloplaacutesticos (Wheeler 1997) con potencial osteoconductivo Todos estos
materiales aunque aceptables no son adecuados en muchas condiciones y generalmente requieren
una consideracioacuten adicional en el proceso de decisioacuten (Wallace and Froum 2003) Ademaacutes la
cantidad y calidad de hueso que se puede obtener con estos materiales a menudo es limitada
El uso de moleacuteculas bioactivas por siacute solas o en combinacioacuten con los materiales descritos
previamente se ha convertido por lo tanto en un aacuterea de intereacutes principal gracias a su alto
potencial Al usar este tipo de procedimientos es importante considerar 1) el meacutetodo de
administracioacuten y 2) la moleacutecula por siacute misma Las moleacuteculas bioactivas pueden transportarse al
24
aacuterea del defecto como una solucioacuten o un gel incrustados en esponjas adheridos a scaffolds soacutelidos
y maacutes recientemente incluidos en partiacuteculas de diferentes tamantildeos Usando estos meacutetodos se
puede acudir a una gran diversidad de biomoleacuteculas como PDGF (factor de crecimiento
derivado de plaquetas) FGF (factor de crecimiento de fibroblastos) IGF (factor de crecimiento
de insulina) RUNX2 osterix (Osx) proteiacutena de mineralizacioacuten de dominio LIM (LMP) BMP
(proteiacutena morfogeacutenica oacutesea) y maacutes recientemente periostin como candidatos potenciales para los
procedimientos de regeneracioacuten dentro de la cavidad oral incluidos los tejidos oacuteseos y
periodontales (Padial-Molina and Rios 2014) (Padial-Molina Volk and Rios 2014) Estas
moleacuteculas se probaron solas o en combinacioacuten con ceacutelulas madre (Behnia et al 2012) utilizando
varias estrategias in vitro e in vivo (Padial-Molina et al 2012)
11 BMPs Accioacuten y regulacioacuten
En regeneracioacuten oacutesea y en particular los factores de crecimiento morfogeneacuteticos oacuteseos (BMP)
son probablemente el grupo de moleacuteculas maacutes comuacuten Desde 1965 cuando Urist (Urist 1965)
demostroacute que las BMPs oacuteseas extraiacutedas podriacutean inducir la formacioacuten de hueso y cartiacutelago cuando
se implantan en tejido animal un alto nuacutemero de artiacuteculos han probado su aplicacioacuten in vivo y su
base bioloacutegica cuando se usan en defectos oacuteseos (Boyne and Jones 2004) (Wang et al 1990)
(Wozney 1992) Las BMPs son miembros de la suacuteper familia de proteiacutenas TGF-β (Barboza
Caula and Machado 1999) La familia de proteiacutenas BMP agrupa maacutes de 20 proteiacutenas
morfogeneacuteticas homodimeacutericas o heterodimeacutericas que funcionan en muchos tipos y tejidos
celulares no todos tienen que ser necesariamente osteogeacutenicos (Ana Claudia Carreira et al
2014) Las BMPs se pueden dividir en 4 subfamilias seguacuten su funcioacuten y secuencia siendo BMP-
2 -4 y -7 las que tienen un fuerte potencial osteogeacutenico (Ana Claudia Carreira et al 2014) Las
acciones de las BMPs incluyen la condrogeacutenesis la osteogeacutenesis la angiogeacutenesis y la siacutentesis de
la matriz extracelular (Bustos-Valenzuela et al 2011) Dentro de esta familia de proteiacutenas BMP-
2 ha sido la maacutes estudiada Tiene propiedades osteoinductoras que promueven la formacioacuten de
25
nuevo hueso al iniciar estimular y amplificar la cascada de la formacioacuten oacutesea a traveacutes de la
quimiotaxis y la estimulacioacuten de la proliferacioacuten y diferenciacioacuten del linaje celular osteoblaacutestico
(Myeroff and Archdeacon 2011) (Boyne and Jones 2004) (Wozney 1992) (Barboza Caula and
Machado 1999) La ausencia de eacutesta como se estudioacute en los modelos eliminatorios conduce a
fracturas espontaacuteneas que no cicatrizan con el tiempo (Tsuji et al 2006) De hecho otros modelos
han demostrado que la ausencia de cualquiera de estas dos BMP-4 (Tsuji et al 2008) o -7 (Tsuji
et al 2010) no conducen a la formacioacuten de hueso y deterioro como demuestra el efecto producido
por BMP-2 sola (Chen Deng and Li 2012)
Muchos tipos de ceacutelulas en el tejido oacuteseo producen BMP como las ceacutelulas osteoprogenitoras
osteoblastos condrocitos plaquetas y ceacutelulas endoteliales Esta BMP secretada se almacena en la
matriz extracelular donde interactuacutea principalmente con el colaacutegeno tipo IV (Ramel and Hill
2012) Durante los procesos de reparacioacuten y remodelacioacuten la actividad absorbente de los
osteoclastos induce la liberacioacuten de BMP al medio para que se suspenda la funcioacuten de absorcioacuten
y eacutesta pueda interactuar con las ceacutelulas cercanas para iniciar el consecuente proceso osteogeacutenico
(A C Carreira et al 2014)
La BMP en la matriz extracelular se une a los receptores de la superficie celular BMPR-I y II
y activa las proteiacutenas citoplasmaacuteticas Smad o la viacutea MAPK (Deschaseaux Sensebe and Heymann
2009) Cuando BMPR-I se activa BMPR-II se engancha y se activa tambieacuten (Mueller and Nickel
2012) La activacioacuten del complejo BMPR-I y BMPR-II conduce a la activacioacuten de varios Smads
(1 5 y 8) que tambieacuten activan Smad-4 y todos forman complejos proteicos que se transportan al
nuacutecleo donde Runx2 Dlx5 y los genes Osterix (importantes en la osteogeacutenesis) se activan (Chen
Deng and Li 2012) (Ramel and Hill 2012) (Figura 1) De forma similar cuando se activa la ruta
de MAPK conduce a la induccioacuten de la transcripcioacuten de Runx2 y por lo tanto a la diferenciacioacuten
oacutesea (Sieber et al 2009) Tambieacuten se han descrito varios antagonistas extracelulares e
intracelulares que incluyen noggin chordin y gremlin o Smad-6 -7 y -8b respectivamente
(Sapkota et al 2007)
26
Figura 1 Representacioacuten esquemaacutetica de la ruta molecular principal de BMP a la
osteogeacutenesis Las BMP interactuacutean con los receptores de la superficie celular I y II para activar
Smads 1 5 y 8 Estos Smads activados activan Smad 4 Todos juntos como un complejo de
proteiacutenas activan Runx2 Dlx5 y Osterix
Foto tomada de Articulo Ortega-Oller I Padial-Molina M Galindo-Moreno P OacuteValle F
Jodar-Reyes AB Peula-Garcia JM Bone regeneration form Plga Micro-Nanoparticles BioMed
Research International 2015 415289 (2015)
27
111Uso cliacutenico de la BMP-2
Hoy en diacutea la BMP-2 estaacute disponible comercialmente bajo diferentes nombres de marcas y
concentraciones Por lo general consiste en una esponja absorbible de colaacutegeno fijada con BMP-
2 humana recombinante En 2002 fue aprobado por la FDA como una alternativa de injerto oacuteseo
autoacutegeno en la fusioacuten intersomaacutetica lumbar anterior (McKay Peckham and Badura 2007) Maacutes
tarde en 2007 la FDA aproboacute el uso de rhBMP-2 como una alternativa para el injerto oacuteseo
autoacutegeno en el aumento de los defectos de la cresta alveolar asociados con la extraccioacuten del diente
en la neumatizacioacuten del seno maxilar (McKay Peckham and Badura 2007)
Ademaacutes de las aplicaciones en estudios cliacutenicos de columna donde se usan concentraciones
muy altas (AMPLIFYTM rhBMP-2 40 mg) los estudios cliacutenicos han apoyado su uso en la
cavidad oral Las BMP se han utilizado en la regeneracioacuten periodontal la terapeacuteutica oacutesea la
osteointegracioacuten de implantes la cirugiacutea oral con fines ortodoacutencicos la reparacioacuten de secuelas
derivadas de la patologiacutea oacutesea la osteogeacutenesis por distraccioacuten y la cirugiacutea reparadora de
endodoncia (A C Carreira et al 2014) (Hong et al 2013) Sin embargo han mostrado resultados
maacutes prometedores en casos en los que solo se regeneraraacute el tejido oacuteseo incluido el desarrollo del
sitio pre-implantario la elevacioacuten de seno el aumento de cresta vertical y horizontal y la
cicatrizacioacuten de cirugiacuteas de implantes dentales (Spagnoli and Marx 2011) En este sentido se
evidencioacute que el uso de rhBMP-2 indujo la formacioacuten de hueso adecuado para la colocacioacuten de
implantes dentales y su osteointegracioacuten (Nevins et al 1996) Ademaacutes parece que el hueso recieacuten
formado tiene propiedades similares al hueso nativo y por lo tanto es capaz de soportar las
fuerzas oclusales que ejerce la dentadura durante su funcioacuten masticatoria (Boyne et al 2005)
En resumen los estudios nombrados concluyeron que rhBMP-2 induce la formacioacuten de nuevo
hueso con una calidad y cantidad comparable al inducido por la cicatrizacioacuten del propio paciente
e incluso en algunos de los casos se informoacute de haber obtenido una cantidad y calidad de hueso
mayor a la que se hubiese obtenido por la viacutea de cicatrizacioacuten normal del paciente (Lee et al
2013)
28
Por el contrario estudios recientes revelan graves complicaciones despueacutes de su uso (Ronga et
al 2013) Ademaacutes se han asociado efectos carcinogeacutenicos a altas dosis lo que llevoacute a los autores
a enfatizar en la necesidad de mejores pautas en el uso cliacutenico de BMP (Devine et al 2012) No
tan draacutesticos son los uacuteltimos estudios que destacan los efectos secundarios negativos y los riesgos
de su aplicacioacuten haciendo gran hincapieacute en el sesgo potencial de la investigacioacuten patrocinada por
la industria no reproducible especialmente cuando se utiliza en la meacutedula espinal (Fu et al 2013)
(Carragee Hurwitz and Weiner 2011) (Simmonds et al 2013) Se observoacute tambieacuten que el uso
de rhBMP-2 aumenta el riesgo de complicaciones en la zona tratada disfagia con alta eficacia y
dantildea la tergiversacioacuten mediante informes selectivos publicaciones duplicadas y subregistros (Fu
et al 2013) Especiacuteficamente en el campo de la regeneracioacuten oacutesea dentro de la cavidad oral un
estudio de elevacioacuten de seno concluyoacute que el uso de BMP-2 promueve efectos negativos en la
formacioacuten oacutesea cuando se combina con matriz oacutesea bovina inorgaacutenica vs hueso bovino
inorgaacutenico solo (Kao et al 2012) en contraste con artiacuteculos y revisiones previas (Torrecillas-
Martinez et al 2013) Al tomar en cuenta esta informacioacuten se puede concluir que es de extrema
importancia tener cuidado con el uso cliacutenico de nuevos productos evitando las aplicaciones no
clasificadas Tambieacuten es importante resaltar la necesidad de maacutes y mejores investigaciones
cliacutenicas
Para superar estas limitaciones el uso de ceacutelulas mesenquimales especificas (MSC) autoacutelogas
modificadas por BMP-2 ex vivo (Chung et al 2012) en los uacuteltimos antildeos estaacute dando lugar a
explorar nuevas estrategias como la encapsulacioacuten de la proteiacutena en diferentes biomateriales o el
suministro mediante terapia geacutenica
El desarrollo de estas tecnologiacuteas se basa en algunos hechos bioloacutegicos Los efectos in vitro de
las BMP se observan en dosis muy bajas (5-20 ngml) aunque las rhBMP actuales disponibles
comercialmente se usan en dosis grandes (hasta 40 mg de algunos productos) (A C Carreira et al
2014) Esto probablemente se deba a un consumo proteoliacutetico intenso durante las primeras fases
posquiruacutergicas Es importante conocer la secuencia adecuada de los procesos bioloacutegicos que
29
conducen a la cicatrizacioacuten normal del tejido Por lo tanto este conocimiento se puede usar para
intervenir en el marco temporal especiacutefico en el que se pretende que actuacutee nuestra terapia (Padial-
Molina et al 2012) Tambieacuten es importante tener en cuenta que el papel de otras viacuteas moleculares
y la diafoniacutea entre los diferentes componentes que llevan a cabo la regeneracioacuten oacutesea todaviacutea no
se entiende perfectamente y por lo tanto se debe realizar maacutes investigacioacuten
Lo que hasta ahora se sabe en resumen es que las BMP y especiacuteficamente BMP-2 son uacutetiles
para promover la regeneracioacuten oacutesea (A C Carreira et al 2014) Sin embargo las rutas disponibles
de administracioacuten local basadas en la activacioacuten de las BMP entregadas por esponjas de colaacutegeno
presentan importantes limitaciones (Chung et al 2012) En primer lugar la proteiacutena se inactiva
raacutepidamente Por lo tanto su accioacuten bioloacutegica desaparece puede ser incluso antes de que se forme
el coaacutegulo de sangre el cual se forma despueacutes de la cirugiacutea Ademaacutes la distribucioacuten de la BMP
en una suspensioacuten liacutequida incrustada en una esponja de colaacutegeno hace que sea imposible estar
seguro de que la proteiacutena estaacute alcanzando el objetivo ideal Debido a eso deben desarrollarse
nuevas formas de administracioacuten de BMP-2 Estas nuevas tecnologiacuteas tienen que garantizar una
mayor vida media de la proteiacutena y una liberacioacuten escalonada para aumentar los efectos sobre los
objetivos celulares deseados La biotecnologiacutea abre la puerta para poder proporcionar una
solucioacuten a estas limitaciones
De esta manera las nanopartiacuteculas biodegradables (nanoesferas y nanocaacutepsulas) fueron
desarrolladas como una herramienta importante y prometedora para la administracioacuten de
macromoleacuteculas a traveacutes de aplicaciones parenterales mucosas y toacutepicas (Barratt 2003)
(Bramwell and Perrie 2005) (Csaba Garcia-Fuentes and Alonso 2006) (M J Santander-Ortega
et al 2010) Los poliacutemeros biodegradables bien establecidos tales como poli (aacutecido D L-laacutectico)
o poli (D L-laacutectico-co-glicoacutelico) se estaacuten utilizando ampliamente en la preparacioacuten de
nanopartiacuteculas en las uacuteltimas deacutecadas debido a su biocompatibilidad y biodegradabilidad
completa (Jiang et al 2005) Sin embargo se sabe que ciertas macromoleacuteculas como proteiacutenas
o peacuteptidos pueden perder actividad durante su encapsulacioacuten almacenamiento administracioacuten y
30
liberacioacuten (Kumar Soppimath and Nachaegari 2006) Para superar este problema la adicioacuten de
estabilizadores tales como oacutexido de polietileno (PEO) o la co-encapsulacioacuten con otras
macromoleacuteculas y sus derivados parece ser una estrategia prometedora
12 Partiacuteculas coloidales polimeacutericas para encapsular moleacuteculas hidrofiacutelicas
Generalmente las partiacuteculas coloidales polimeacutericas son sistemas consistentes con una forma
esfeacuterica homogeacutenea compuesta por poliacutemeros naturales o sinteacuteticos Con el fin de encapsular
moleacuteculas hidroacutefilas como proteiacutenas o aacutecidos nucleicos para ello es necesario optimizar la
composicioacuten polimeacuterica y el meacutetodo de siacutentesis En este proceso se debe lograr una alta eficacia
de encapsulacioacuten el mantenimiento de la actividad bioloacutegica de la biomoleacutecula encapsulada y la
obtencioacuten de un patroacuten de liberacioacuten adecuado (Danhier et al 2012) (Kumari Yadav and Yadav
2010) (Makadia and Siegel 2011) Varios sistemas de administracioacuten de BMP2 (y otros GF) que
usan partiacuteculas polimeacutericas estaacuten descritos en la bibliografiacutea La mayoriacutea de ellos son sistemas
microparticulados Casi en su totalidad todos ellos usan el copoliacutemero de PLGA biocompatible
y biodegradable como componente principal (Mohamed and van der Walle 2008) (Silva et al
2007)
Teniendo en cuenta la incorporacioacuten de BMP2 al sistema portador la encapsulacioacuten es
preferible a la adsorcioacuten porque los factores de crecimiento estaacuten maacutes protegidos contra factores
ambientales en el medio y pueden tener un mejor control sobre la administracioacuten y liberacioacuten para
alcanzar las concentraciones deseadas en sitio y tiempo especiacuteficos (Zhang and Uludag 2009)
Normalmente si los GF estaacuten relacionados con los procesos de regeneracioacuten oacutesea las nano-
micropartiacuteculas quedan atrapadas en un segundo sistema como hidrogeles o scaffolds de
ingenieriacutea tisular que tambieacuten juegan un papel importante en el perfil de liberacioacuten de los GF de
estas partiacuteculas (Zhang and Uludag 2009) Las nano-micropartiacuteculas han permitido el desarrollo
de scaffolds multiescala lo que facilita el control de la arquitectura interna y los patrones
31
adecuados de los gradientes mecaacutenicos de las ceacutelulas asiacute como los factores de sentildealizacioacuten (Santo
et al 2012)
Todos los pasos desde el meacutetodo de siacutentesis y sus caracteriacutesticas el proceso de encapsulacioacuten
o la modificacioacuten final de la superficie para una entrega dirigida determinan las caracteriacutesticas de
estos sistemas y su objetivo principal la liberacioacuten controlada de GF bioactivos
Figura 2 Procedimiento de doble emulsioacuten (emulsioacuten agua aceite agua W1OW2) para
obtener micropartiacuteculas nanopartiacuteculas de PLGA
En la figura 2 se muestra el esquema de siacutentesis de micro-nanopartiacuteculas de PLGA mediante
un procedimiento de doble emulsioacuten Dependiendo de las condiciones de siacutentesis (estabilizadores
disolventes y procedimiento de mezcla) es posible obtener micro-nanoesferas con una matriz
uniforme o micro-nanocaacutepsulas con una estructura corteza-nuacutecleo Las inmunopartiacuteculas
32
utilizadas para la administracioacuten dirigida pueden obtenerse uniendo moleacuteculas especiacuteficas de
anticuerpos en la superficie de la partiacutecula
33
121Meacutetodos de siacutentesis
Es posible encontrar varios procedimientos para encapsular moleacuteculas hidrofiacutelicas como
proteiacutenas o aacutecidos nucleicos en nano micropartiacuteculas polimeacutericas Se ha observado que las
teacutecnicas de separacioacuten de fases (Tran Swed and Boury 2012) o secado por pulverizacioacuten (Ertl et
al 2000) encapsulan moleacuteculas hidroacutefilas Sin embargo en el caso de las proteiacutenas el
procedimiento maacutes utilizado para encapsularlas en micropartiacuteculas y nanopartiacuteculas de PLGA es
la teacutecnica de evaporacioacuten con disolvente de doble emulsioacuten (WOW) (Makadia and Siegel 2011)
(Hans and Lowman 2002) En la figura 2 se presenta una descripcioacuten esquemaacutetica de esta teacutecnica
De manera general el PLGA se disuelve en un disolvente orgaacutenico y se emulsiona usando
agitacioacuten mecaacutenica o sonicacioacuten con agua que contiene una cantidad apropiada de proteiacutena
Por lo tanto se obtiene una emulsioacuten W1O primaria En la segunda fase esta emulsioacuten se
vierte en una gran fase polar que conduce a una precipitacioacuten inmediata de las partiacuteculas como
consecuencia de la contraccioacuten del poliacutemero alrededor de las gotitas de la emulsioacuten primaria Esta
fase puede estar compuesta por una solucioacuten acuosa de un estabilizador (surfactante) o mezclas
de etanol y agua (Blanco and Alonso 1998) (Csaba et al 2005) Despueacutes de agitar el resultado
del disolvente orgaacutenico se extrae raacutepidamente por evaporacioacuten al vaciacuteo En este procedimiento se
ha probado una amplia lista de diferentes modificaciones con el fin de obtener un sistema micro
nanoportador con estabilidad coloidal adecuada alta eficacia de encapsulacioacuten bioactividad
adecuada y finalmente un perfil de liberacioacuten a largo plazo con una miacutenima descarga inicial
El objetivo es evitar que se libere una gran cantidad de proteiacutena (gt 60) muy raacutepidamente (24
horas) que es uno de los mayores problemas de un sistema de liberacioacuten controlada (Mohamed
and van der Walle 2008)
34
122 Disolvente orgaacutenico
Hans y colaboradores muestran diferentes ejemplos de solventes orgaacutenicos usados en muacuteltiples
procesos de emulsioacuten Normalmente pueden usarse diclorometano (DMC) acetato de etilo
acetona y otras mezclas (Hans and Lowman 2002) En el primer paso seriacutea una buena eleccioacuten
escoger un buen solvente orgaacutenico con baja solubilidad en agua para facilitar el proceso de
emulsioacuten y bajo punto de ebullicioacuten para una faacutecil evaporacioacuten Sin embargo la estructura de las
moleacuteculas de proteiacutenas encapsuladas puede verse afectada y los procesos de desnaturalizacioacuten y
peacuterdida de actividad bioloacutegica aparecen cuando interactuacutean con un solvente orgaacutenico tiacutepico como
DMC (Danhier et al 2012) El acetato de etilo por otro lado ejerce efectos menos
desnaturalizantes con una menor incidencia en la bioactividad de las proteiacutenas encapsuladas
(Sturesson and Carlfors 2000)
Otros factores importantes relacionados con el disolvente orgaacutenico son sus propiedades fiacutesicas
que afectan la forma en que las moleacuteculas del poliacutemero se auto organizan en la envoltura de las
gotas de la emulsioacuten y modifican la morfologiacutea de las nanopartiacuteculas y la eficacia de
encapsulacioacuten (Rosca Watari and Uo 2004) De esta forma una mayor solubilidad en agua del
disolvente orgaacutenico es decir acetato de etilo favorece una eliminacioacuten maacutes raacutepida del disolvente
Ademaacutes la velocidad de eliminacioacuten del disolvente puede controlarse ajustando el volumen de la
fase polar asiacute como la tensioacuten de cizallamiento durante la segunda etapa de la emulsioacuten Un
aumento de estos dos paraacutemetros aumenta la velocidad de difusioacuten del acetato de etilo desde las
micropartiacuteculas primarias a la fase acuosa externa lo que da como resultado su raacutepida
solidificacioacuten (Meng et al 2003) Tambieacuten mejora la eficiencia de encapsulacioacuten y minimiza el
tiempo de contacto entre las moleacuteculas de proteiacutena y el solvente orgaacutenico (Ghaderi and Carlfors
1997) obteniendo al mismo tiempo un menor efecto de raacutefaga y una liberacioacuten maacutes lenta del
faacutermaco desde las micropartiacuteculas (Meng et al 2003)
35
123 Tamantildeo y morfologiacutea de las partiacuteculas
El tamantildeo de la partiacutecula es un paraacutemetro importante y uno de los objetivos principales del
sistema de liberacioacuten polimeacuterica Las microesferas desde unos pocos microacutemetros hasta 100 μm
son adecuadas para el suministro oral la adhesioacuten a la mucosa o el uso interior del armazoacuten es
decir para la regeneracioacuten oacutesea La dimensioacuten a nano-escala del soporte ofrece una versatilidad
mejorada cuando se compara con partiacuteculas de mayor tamantildeo Esto se debe a que tienen una
mayor estabilidad coloidal una mejor dispersabilidad y biodisponibilidad una superficie maacutes
reactiva y ademaacutes pueden administrar proteiacutenas o faacutermacos dentro y fuera de las ceacutelulas
correspondientes (Wang et al 2012) BMP2 promueve la formacioacuten de hueso e induce la
expresioacuten de otras BMP e inicia la viacutea de sentildealizacioacuten desde la superficie de la ceacutelula unieacutendose
a dos receptores de superficie diferentes (Bustos-Valenzuela et al 2011) Por lo tanto las
partiacuteculas portadoras de BMP2 deben liberarlo en el medio extracelular Dado que la ingesta
celular de nanopartiacuteculas de PLGA es muy raacutepida el proceso de incorporacioacuten puede verse
limitado por un aumento en el tamantildeo de nano a micropartiacuteculas (Xiong et al 2011) Sin
embargo la interaccioacuten entre partiacuteculas y ceacutelulas estaacute fuertemente influenciada por el tamantildeo de
la partiacutecula Si se desea la internalizacioacuten de la ceacutelula la partiacutecula debe estar comprendida en la
escala submicroacutemica en un intervalo entre 2-500 nm (Chou Ming and Chan 2011) Ademaacutes este
tamantildeo es necesario para una distribucioacuten raacutepida despueacutes de la administracioacuten parenteral con el
fin de alcanzar diferentes tejidos a traveacutes de diferentes barreras bioloacutegicas Ademaacutes la ingesta de
macroacutefagos se minimiza con un diaacutemetro de nanopartiacuteculas por debajo de 200 nm e incluso maacutes
pequentildeo (Hans and Lowman 2002) (Manuel J Santander-Ortega Lozano-Loacutepez et al 2010)
Como se discutioacute en un artiacuteculo escrito por Yang y colaboradores (Yang Chung and Ng 2001)
ligeras modificaciones del procedimiento de siacutentesis pueden suponer efectos draacutesticos en el
tamantildeo o la morfologiacutea de las partiacuteculas y por lo tanto en la eficacia de encapsulacioacuten de
proteiacutenas y la liberacioacuten cineacutetica
36
Figura 3 Fotografiacutea mediante microscopiacutea electroacutenica de barrido (SEM) de nanopartiacuteculas
de PLGA obtenidas mediante un procedimiento de emulsificacioacuten de doble emulsioacuten Es un
sistema con forma esfeacuterica baja polidispersidad y una escala nanoscoacutepica que muestra las
propiedades deseadas para una distribucioacuten fisioloacutegica adecuada y la internalizacioacuten celular
En los procesos de doble emulsioacuten la primera etapa de emulsioacuten determina en gran medida el
tamantildeo de la partiacutecula mientras que la segunda etapa de emulsioacuten caracterizada por la
eliminacioacuten del disolvente y la precipitacioacuten del poliacutemero afecta principalmente a la morfologiacutea
de la partiacutecula (Rosca Watari and Uo 2004) Sin embargo en este paso el uso de soluciones
surfactantes como el medio polar del segundo proceso de emulsioacuten y la relacioacuten de volumen entre
las fases orgaacutenicas y polares han mostrado una influencia importante en el tamantildeo final (Feczkoacute
Toacuteth and Gyenis 2008)Por lo tanto la eleccioacuten correcta del solvente orgaacutenico la concentracioacuten
del poliacutemero la adicioacuten de surfactante y la energiacutea del proceso de emulsioacuten permiten controlar el
tamantildeo del sistema
37
La incorporacioacuten de poloxaacutemeros (F68) en el disolvente orgaacutenico de la emulsioacuten primaria
ayuda a aumentar la estabilidad coloidal de la primera dispersioacuten al colocarla en el interfaz WO
Esto reduce el tamantildeo de partiacutecula en comparacioacuten con las nanopartiacuteculas de PLGA puro en las
que la uacutenica fuente de estabilidad proviene de la carga eleacutectrica de los grupos carboxilo del PLGA
(Santander-Ortega Bastos-Gonzalez and Ortega-Vinuesa 2007) Es normal obtener micro
nanoesferas ciliacutendricas con un nuacutecleo poroso polimeacuterico En la figura 3 se muestra una
micrografiacutea SEM tiacutepica de nanopartiacuteculas de PLGA obtenidas mediante emulsioacuten WOW usando
una mezcla de disolventes orgaacutenicos (DCM acetona) y etanol agua como segundo medio polar
en la que la forma esfeacuterica y la distribucioacuten uniforme del tamantildeo son las principales
caracteriacutesticas La cubierta exterior polimeacuterica en la segunda etapa de emulsioacuten empujoacute las gotas
de agua hacia el nuacutecleo interno de acuerdo con el proceso de solidificacioacuten (Yang Chia and
Chung 2000) Este proceso permite producir partiacutecula como son estas caacutepsulas con una
estructura nuacutecleo-capa en la que el nuacutecleo interno tiene una baja densidad de poliacutemero
La figura 4 muestra una estructura nuacutecleo-capa tiacutepica en la que el poliacutemero precipita y se
contrae alrededor de las gotas de agua durante el cambio de disolvente de la segunda fase y el
posterior proceso de evaporacioacuten del disolvente orgaacutenico (Fang et al 2014) En este caso el
proceso de solidificacioacuten del poliacutemero se ve influenciado y determinado por la miscibilidad del
disolvente orgaacutenico con la segunda fase polar y la velocidad de eliminacioacuten
38
Figura 4 Nanopartiacuteculas de mezcla PLGA poloxamers188 (a) Fotografiacutea de microscopiacutea
electroacutenica de transmisioacuten (STEM) (b) Fotografiacutea de microscopiacutea electroacutenica de barrido (SEM)
La teacutecnica STEM permite el anaacutelisis de la estructura de nanopartiacuteculas con una regioacuten interna
con baja densidad de poliacutemero que es representativa de nanocaacutepsulas con estructura nuacutecleo-
caparazoacuten
La cubierta polimeacuterica a menudo presenta canales o poros como consecuencia de la extrusioacuten
de agua interna debido a las fuerzas osmoacuteticas Esto puede reducir la eficacia de la encapsulacioacuten
y favorecer una raacutepida fuga inicial con la liberacioacuten en raacutefaga no deseada (Yang Chung and
Ng 2001) Esta modificacioacuten de la estructura interna de las partiacuteculas generalmente se indica
asignando el teacutermino nanoesfera al sistema con un nuacutecleo que consiste en una matriz polimeacuterica
homogeacutenea El agente bioactivo se dispersa dentro de ellas mientras que la estructura nuacutecleo-
capa seriacutea similar a una nanocaacutepsula donde la biomoleacutecula estaacute preferiblemente en la cavidad
acuosa rodeada por la cubierta polimeacuterica (Zhang and Uludag 2009) (ver figura 2)
39
13Agentes estabilizadores
131 Estabilidad coloidal
El meacutetodo de doble emulsioacuten normalmente requiere la presencia de estabilizadores para
conferir estabilidad coloidal durante la primera etapa de emulsioacuten para evitar la coalescencia de
las gotas de la emulsioacuten y maacutes tarde para mantener la estabilidad de las nano micropartiacuteculas
finales (Ratzinger et al 2010) El alcohol poliviniacutelico (PVA) y el derivado de PEO como
poloxaacutemeros se han usado en la mayoriacutea de los casos (Blanco and Alonso 1998) (Feczkoacute Toacuteth
and Gyenis 2008) Otros incluyen surfactantes naturales como los fosfoliacutepidos (Feng and Huang
2001) (Chan et al 2009) En algunos casos es posible evitar los surfactantes si las partiacuteculas
tienen una contribucioacuten de estabilidad electrostaacutetica es decir de los grupos carboxilo terminales
no protegidos de las moleacuteculas de PLGA (Fraylich et al 2008)
Como se ha comentado anteriormente el PVA y los poloxaacutemeros han demostrado su eficacia
en la siacutentesis de nanopartiacuteculas y micropartiacuteculas afectando no solo la estabilidad de los sistemas
sino tambieacuten su tamantildeo y morfologiacutea Por lo tanto se ha encontrado un efecto de reduccioacuten de
tamantildeo usando PVA en la fase acuosa externa que afecta al mismo tiempo la porosidad
superficial principalmente en partiacuteculas de tamantildeo micro (Feczkoacute Toacuteth and Gyenis 2008) Un
estudio comparativo entre PVA y fosfoliacutepidos (di-palmitoil fosfatidilcolina) como estabilizadores
mostroacute que DPPC podriacutea ser un mejor emulsionante que PVA para producir nano y
micropartiacuteculas Con este meacutetodo se necesitaba una cantidad de estabilizador mucho maacutes baja
para obtener un tamantildeo similar En el mismo estudio se demostroacute una mayor porosidad en la
superficie de la partiacutecula para las nanoesferas emulsionadas con PVA (Feng and Huang 2001)
Por otro lado la combinacioacuten de PLGA con poloxaacutemeros ha mostrado efectos positivos para
los nano y microsistemas en teacuterminos de estabilidad eficacia de encapsulacioacuten o caracteriacutesticas
de liberacioacuten controlada (Santander-Ortega et al 2011) El uso de estos surfactantes en el primer
o segundo paso del procedimiento de emulsioacuten WOW conduce a situaciones diferentes Por lo
40
tanto si los poloxaacutemeros se mezclan con PLGA en la fase orgaacutenica de la emulsioacuten primaria se
obtiene una alteracioacuten de la rugosidad superficial Sin embargo si se agregan en la fase de agua
interna se encuentra un aumento de la porosidad (Blanco and Alonso 1998) La inclusioacuten de
poloxaacutemeros en la fase polar de la segunda etapa de emulsioacuten tambieacuten genera superficies de
rugosidad hidroacutefila Una cuantificacioacuten de esto se muestra en la figura 5 en la que se mide la
movilidad electroforeacutetica de PLGA puro y PLGA pluronic F68 nanopartiacuteculas como una funcioacuten
del pH del medio La composicioacuten de superficie diferente afecta el comportamiento
electrocineacutetico de las nanopartiacuteculas desnudas La carga superficial se modula por la presencia de
tensioactivo no ioacutenico como poloxaacutemeros o en mayor medida por la presencia de moleacuteculas de
anticuerpos unidos en la superficie La dependencia observada con este paraacutemetro es una
consecuencia del caraacutecter aacutecido deacutebil de los grupos carboxilo de PLGA Cuando las moleacuteculas de
poloxaacutemero estaacuten presentes en la interfaz se encuentra una reduccioacuten sistemaacutetica de la movilidad
como consecuencia del aumento de la rugosidad superficial Las cadenas de surfactante hidroacutefilo
se dispersan hacia el disolvente originando un desplazamiento del plano de corte y la consecuente
reduccioacuten de movilidad (Santander-Ortega Bastos-Gonzalez and Ortega-Vinuesa 2007)
(Fraylich et al 2008) La presencia de moleacuteculas proteicas en la superficie introduce una
dependencia de la carga neta superficial con el punto isoeleacutectrico de eacutestas situacioacuten que se refleja
en el comportamiento electrocineacutetico que incluso muestras valores positivos en pHs inferiores al
punto isoeleacutectrico del anticuerpo
El tamantildeo final de las partiacuteculas de PLGA se controla principalmente por fuerzas
electrostaacuteticas y no se ve significativamente afectado por la presencia o la naturaleza de los
estabilizadores de poloxaacutemero (Fraylich et al 2008) El reconocimiento de los nanovehiacuteculos por
el sistema fagociacutetico mononuclear (MPS) se puede alterar significativamente si la superficie de
las partiacuteculas coloidales se modifica mediante el uso de copoliacutemeros de bloque PEO de las
moleacuteculas de poloxaacutemero La barrera esteacuterica proporcionada por estas moleacuteculas surfactantes
previenen o minimizan la adsorcioacuten de proteiacutenas plasmaacuteticas y disminuye el reconocimiento por
41
los macroacutefagos (Tan et al 1993) El tamantildeo de las microesferas tampoco se ve afectado por la
encapsulacioacuten de poloxaacutemeros El sistema que contiene mezclas de poloxaacutemero-PLGA conduce
a una estructura interna que muestra pequentildeos orificios y cavidades en relacioacuten con microesferas
de PLGA puro con una estructura de tipo matriz compacta (Blanco and Alonso 1998)
Figura 5 Movilidad electroforeacutetica versus pH para nanopartiacuteculas de PLGA con diferentes
caracteriacutesticas () PLGA (◼) mezcla de PLGA poloxamer188 y () PLGA cubierto por
Immuno-γ-globulina
Las micropartiacuteculas formuladas por poloxaacutemero en el segundo medio polar tienen una
superficie completamente diferente que las de PVA casi sin poros (Feczkoacute Toacuteth and Gyenis
2008) Una comparacioacuten entre diferentes poloxaacutemeros muestra que el balance hidroacutefilo-lipoacutefilo
(HBL) del surfactante juega un papel crucial determinando las interacciones surfactante-poliacutemero
y controlando la porosidad y la rugosidad de las nano-micropartiacuteculas (Blanco and Alonso 1998)
(Bouissou et al 2004)
42
De manera similar a los surfactantes las caracteriacutesticas del poliacutemero como el grado de
hidrofobicidad el peso molecular o la velocidad de degradacioacuten de la hidroacutelisis pueden influir
fuertemente en la morfologiacutea de la partiacutecula Por lo tanto la composicioacuten polimeacuterica de las
partiacuteculas afecta en gran medida su estructura y propiedades Es por eso que es habitual usar otros
poliacutemeros para modificar el comportamiento y la aplicacioacuten de las partiacuteculas De esta manera el
polietilenglicol (PEG) de diferente longitud de cadena se usa frecuentemente para modificar las
caracteriacutesticas de la superficie Con PEG las partiacuteculas son maacutes hidroacutefilas y tienen superficies
maacutes rugosas que afectan la accioacuten de MPS al aumentar el tiempo de circulacioacuten y la vida media
in vivo como la presencia de cadenas de PEO (Gref et al 1994) Ademaacutes las cadenas de PEG
tambieacuten proporcionan estabilidad coloidal a traveacutes de la estabilizacioacuten esteacuterica Las
nanopartiacuteculas o micropartiacuteculas de PLGA se pueden obtener normalmente mediante el uso en el
meacutetodo de siacutentesis de copoliacutemeros de di y tri-bloque de PLGA PEG (Lochmann et al 2010)
(White et al 2013) (Makadia and Siegel 2011) Los poliacutemeros naturales como el quitosano
ademaacutes de aumentar la hidrofobicidad de la superficie tambieacuten les confieren un caraacutecter
mucoadherente (Paolicelli et al 2010)
132 Eficacia de encapsulacioacuten y bioactividad
Ademaacutes el uso de estabilizantes (surfactantes o poliacutemeros) tambieacuten influye en la eficacia de
encapsulacioacuten y la estabilidad de la proteiacutena De hecho para el proceso de evaporacioacuten del
solvente WOW el solvente orgaacutenico clorado usado para la primera emulsioacuten puede degradar las
moleacuteculas de proteiacutena encapsuladas en este paso si entran en contacto con la interfaz orgaacutenica
agua causando su agregacioacuten o desnaturalizacioacuten (Brigger Dubernet and Couvreur 2002) La
interaccioacuten poliacutemero-proteiacutena el estreacutes de cizallamiento para el proceso de emulsioacuten y la
reduccioacuten del pH derivada de la degradacioacuten del poliacutemero PLGA tambieacuten pueden producir la
misma situacioacuten con la peacuterdida posterior de la actividad bioloacutegica de las biomoleacuteculas
encapsuladas Se han usado diferentes estrategias para prevenirlo Por ejemplo un aumento de la
viscosidad alrededor de las moleacuteculas de proteiacutenas puede ayudar a aislarlas de su microambiente
43
(Giteau et al 2008) De esta forma los productos viscosos como el almidoacuten se han utilizado
para prevenir la inestabilidad proteica (Balmayor et al 2009) Estos autores coencapsulan BMP2
con albuacutemina dentro de micropartiacuteculas de almidoacuten usando otro poliacutemero biodegradable poli-ε-
caprolactona en lugar de PLGA La BMP2 retuvo la bioactividad A pesar de una baja tasa de
encapsulacioacuten ademaacutes de una raacutefaga inicial seguida de una liberacioacuten incompleta la cantidad de
BMP2 necesaria al principio fue menor (Balmayor et al 2009) La combinacioacuten de surfactantes
PEO con PLGA (mezclados en la fase orgaacutenica) tambieacuten puede preservar la bioactividad de las
proteiacutenas microencapsuladas (Santander-Ortega et al 2009) o los aacutecidos nucleicos (Csaba et al
2005)
Sin embargo en la mayoriacutea de los casos la estrategia preferida fue la coencapsulacioacuten de
estabilizadores con biomoleacuteculas De este modo las albuacuteminas seacutericas (SA) han demostrado la
capacidad de limitar la agregacioacuten-desestabilizacioacuten de varias proteiacutenas incitadas por el interfaz
agua disolvente orgaacutenico del proceso de emulsioacuten primaria (Meinel et al 2001) (Srinivasan et
al 2005) White y colaboradores en uno de sus estudios encapsularon lisozima dentro de
micropartiacuteculas de PLGA-PEG Ademaacutes de la funcioacuten de proteccioacuten tambieacuten observaron un
aumento importante de la eficiencia de encapsulacioacuten cuando el SA humana se co-encapsuloacute con
lisozima y BMP2 (White et al 2013) Dr Angelo y colaboradores usaron heparina como
estabilizador porque eacutesta forma un complejo especiacutefico con varios factores de crecimiento
estabiliza su estructura tridimensional y promueve su bioactividad Se consiguioacute aumentar asiacute la
eficacia de encapsulacioacuten del 35 al 87 usando SA bovina como un segundo estabilizador para
encapsular dos factores de crecimiento proangiogeacutenico naturales dentro de las nanopartiacuteculas
mezcladas con PLGA-poloxaacutemero Los ensayos celulares in vitro mostraron la preservacioacuten de la
actividad bioloacutegica de GF hasta un mes despueacutes (drsquoAngelo et al 2010)
El uso de maacutes surfactantes hidroacutefilos (poloxaacutemeros) o poliacutemeros (PEG) en la fase acuosa
interna o durante la mezcla del PLGA en la fase orgaacutenica de la emulsioacuten primaria reduce la
interaccioacuten de las proteiacutenas encapsuladas con la matriz de PLGA hidroacutefoba Esto evita la
44
alteracioacuten de la estructura de las moleacuteculas de proteiacutena y ayuda al mismo tiempo a neutralizar la
acidez generada por la degradacioacuten hidroliacutetica del PLGA (Tan et al 1993) En algunos casos se
ha demostrado que la combinacioacuten de varios estabilizadores como poloxaacutemeros tranexaacutemico y
bicarbonato de sodio preserva la integridad de las proteiacutenas encapsuladas pero tambieacuten reduce
la eficacia de la encapsulacioacuten (Bouissou et al 2004)
Como regla general la eficacia de la encapsulacioacuten aumenta con el tamantildeo de las partiacuteculas
(Hans and Lowman 2002) Ademaacutes la estabilizacioacuten adecuada de la emulsioacuten primaria por
poliacutemeros anfifiacutelicos y una solidificacioacuten (precipitacioacuten) raacutepida del poliacutemero en el segundo paso
son paraacutemetros favorables para mejorar la eficacia de encapsulacioacuten de proteiacutenas en la teacutecnica de
emulsioacuten WOW (Meng et al 2003)
La tendencia de BMP2 a interactuar con superficies hidrofoacutebicas puede disminuir la peacuterdida
de proteiacutena encapsulada durante la liberacioacuten de la fase de disolvente Esto favorece una mayor
encapsulacioacuten pero disminuye la liberacioacuten posterior (Lochmann et al 2010) Se obtiene una
encapsulacioacuten proteica oacuteptima cuando el pH de las fases de agua internas y externas estaacuten cerca
del punto isoeleacutectrico de la proteiacutena (Manuel J Santander-Ortega Lozano-Loacutepez et al 2010)
Blanco y Alonso (Blanco and Alonso 1998) observaron una reduccioacuten en la eficacia de
encapsulacioacuten de proteiacutena cuando el poloxaacutemero se coencapsuloacute en la emulsioacuten primaria Esto
pone de relieve el papel principal desempentildeado por la interaccioacuten proteiacutena-poliacutemero en la eficacia
de encapsulacioacuten y el proceso de liberacioacuten posterior Sin embargo demasiado emulsionante
tambieacuten puede dar como resultado una reduccioacuten de la eficacia de encapsulacioacuten (Feng and
Huang 2001) Por lo tanto se necesita un equilibrio entre el polvo de emulsioacuten del surfactante y
su concentracioacuten
14 Patroacuten de liberacioacuten
El patroacuten de liberacioacuten representa una de las caracteriacutesticas maacutes importantes de un sistema
portador de partiacuteculas nano micro ya que su desarrollo tiene un objetivo final principal la
45
liberacioacuten adecuada de las moleacuteculas bioactivas encapsuladas para alcanzar la accioacuten cliacutenica
deseada
Se han definido diferentes patrones de liberacioacuten de proteiacutenas encapsuladas en micropartiacuteculas
nanopartiacuteculas de PLGA Es posible encontrar una liberacioacuten continua cuando la difusioacuten de la
biomoleacutecula es maacutes raacutepida que la erosioacuten de la partiacutecula Este proceso implica una difusioacuten
continua de la proteiacutena que se encuentra en la matriz del poliacutemero antes de que la partiacutecula de
PLGA se degrade en monoacutemeros de aacutecido laacutectico y glicoacutelico por hidroacutelisis (Kumari Yadav and
Yadav 2010) Tambieacuten se ha descrito una liberacioacuten bifaacutesica caracterizada por una descarga
inicial dentro o cerca de la superficie de la partiacutecula seguido por una segunda fase en la que la
proteiacutena se libera progresivamente por difusioacuten La segunda fase se puede mejorar mediante la
erosioacuten masiva del caparazoacuten y la matriz de PLGA lo que da como resultado un importante
aumento de poros y canales (Makadia and Siegel 2011) Se ha encontrado un tercer perfil de
liberacioacuten trifaacutesica cuando se produce un periacuteodo de liberacioacuten de retardo despueacutes de la descarga
inicial y hasta la degradacioacuten del poliacutemero (Cleland 1997) Finalmente es posible obtener una
liberacioacuten de proteiacutena incompleta como consecuencia de factores adicionales relacionados con la
interaccioacuten proteiacutena-poliacutemero o la inestabilidad proteica La Figura 6 ilustra los diferentes perfiles
de liberacioacuten descritos anteriormente Un sistema de transporte oacuteptimo deberiacutea ser capaz de liberar
un gradiente de concentracioacuten controlado de factores de crecimiento en el momento apropiado
evitando o al menos reduciendo o controlando el efecto de descarga inicial (Oh Kim and Lee
2011) Una explosioacuten inicial controlada seguida de una liberacioacuten sostenida mejora
significativamente la regeneracioacuten oacutesea in vivo (Brown et al 2009) (Brown et al 2011) (Li et
al 2009)
Giteau y colaboradores (Giteau et al 2008) presentan una revisioacuten interesante sobre Coacutemo
lograr una liberacioacuten sostenida y completa de micropartiacuteculas de PLGA Comienzan por analizar
la influencia del medio de liberacioacuten y el meacutetodo de muestreo en el perfil de liberacioacuten y resaltan
la importancia del proceso de limpieza por centrifugacioacuten o el volumen del medio de liberacioacuten
46
Ajustando a valores adecuados la velocidad de centrifugacioacuten o el volumen del tampoacuten es posible
separar micro nanopartiacuteculas del medio de liberacioacuten que contiene proteiacutenas de una manera muy
faacutecil Esto permite patrones de liberacioacuten estables y reproducibles Por otro lado para garantizar
un mejor perfil de liberacioacuten de proteiacutenas se debe realizar la modificacioacuten de la formulacioacuten de
micropartiacuteculas y el proceso de microencapsulacioacuten para preservar la agregacioacuten de proteiacutenas La
estabilidad de la proteiacutena debe mantenerse evitando la formacioacuten de un medio dantildeino Por
ejemplo la formulacioacuten de una siacutentesis en concreto puede modificarse para usar poliacutemeros maacutes
hidroacutefilos ya que se ha demostrado que reducen el estallido inicial y proporcionan proteiacutenas
bioactivas durante largos periodos de tiempo
47
Figura 6 Patroacuten de liberacioacuten (liacutenea negra) Cineacutetica de liberacioacuten de BSA en nanopartiacuteculas
de PLGA con alta liberacioacuten inicial (liacutenea de puntos rojos) modelo bifaacutesico que combina un
estallido inicial moderado y una liberacioacuten sostenida posterior (liacutenea de trazos azules) modelo
trifaacutesico con un retraso de liberacioacuten entre las fases de liberacioacuten inicial y sostenida (liacutenea verde
de guiones) liberacioacuten incompleta
Las estrategias maacutes relevantes se mencionan a continuacioacuten La liberacioacuten de faacutermaco a partir
de nano micropartiacuteculas de PLGA puede controlarse por el peso molecular del poliacutemero y la
relacioacuten entre monoacutemeros (laacutectico glicoacutelico) de forma que un aumento del aacutecido glicoacutelico
acelera la peacuterdida de peso del poliacutemero debido a la mayor hidrofilicidad de la matriz (Makadia
and Siegel 2011)
Por otro lado se ha descrito una erosioacuten maacutes raacutepida de las microesferas con reduccioacuten en el
peso molecular de PLGA debido a la facilidad de penetracioacuten del agua y la posterior degradacioacuten
del poliacutemero (Blanco and Alonso 1998) Schrier y colaboradores trabajaron con microesferas
48
preparadas por WOW utilizando diferentes tipos de PLGA analizaron el importante papel del
peso molecular la relacioacuten laacutectico-glicoacutelico y los residuos de aacutecido (Schrier et al 2001) La
cantidad de rhBMP2 adsorbido en la superficie de la micropartiacutecula aumentoacute con la
hidrofobicidad del poliacutemero Al mismo tiempo la liberacioacuten estaba en correlacioacuten con el perfil
de degradacioacuten de los diferentes poliacutemeros (Schrier et al 2001)
Por lo tanto el uso de poliacutemeros maacutes hidroacutefilos reduce la interaccioacuten proteiacutena hidroacutefoba-
poliacutemero Este efecto favorece una distribucioacuten maacutes homogeacutenea en la matriz polimeacuterica y
aumenta la absorcioacuten de agua en las microesferas Por lo que la velocidad de liberacioacuten de
rhBMP2 encapsulada en microesferas compuestas por un copoliacutemero de di-bloque PEG-PLGA
se incrementa con el contenido de PEG de la matriz de poliacutemero (Lochmann et al 2010) Se
obtuvo un resultado similar utilizando copoliacutemeros tri-bloque PLGA-PEG-PLGA (White et al
2013) En este caso modificando la relacioacuten del monoacutemero (laacutectido-glicoacutelido) en el PLGA y
aumentando la cantidad de PLGA-PEG-PLGA en las formulaciones el patroacuten de liberacioacuten de
BMP-2 co-encapsulada con HSA en microesferas fue ajustable
Por otro lado el uso de mezclas de PLGA-poloxaacutemeros es uacutetil para obtener una liberacioacuten
sostenida durante maacutes de un mes sin incidencia de producirse una descarga inicial (drsquoAngelo et
al 2010) (Manuel J Santander-Ortega Lozano-Loacutepez et al 2010) Sin embargo para un
plaacutesmido encapsulado dentro de nanopartiacuteculas obtenidas mediante mezclas de PLGA-
poloxaacutemero la hidrofobicidad del surfactante permite prolongar la liberacioacuten hasta 2 semanas de
una manera controlada Por otra parte se alcanzoacute una liberacioacuten completa para la mezcla de
PLGA-poloxaacutemero en lugar de nanopartiacuteculas de PLGA en la que la liberacioacuten maacutexima fue de
alrededor del 40 (Csaba et al 2005)
Las mezclas de PLGA y poloxaacutemeros (pluronic F68) tambieacuten se pueden usar para obtener
vesiacuteculas o caacutemaras nanocompuestas mediante un proceso de doble emulsioacuten Estas vesiacuteculas son
adecuadas para la encapsulacioacuten de moleacuteculas hidrofoacutebicas e hidrofiacutelicas La presencia de
pluronic afecta la estabilidad coloidal de las vesiacuteculas y el patroacuten de liberacioacuten de las moleacuteculas
49
encapsuladas Estas vesiacuteculas presentan una pared de 30 nm y el faacutermaco estaacute encapsulado en
presencia del poloxaacutemero (Nair and Sharma 2012)
Otras estrategias incluyen el uso de diferentes compuestos para aumentar el tiempo de
liberacioacuten Por lo tanto BMP2 encapsulada en nanopartiacuteculas de PLGA-PVA (alrededor de 300
nm) mostroacute una mayor eficiencia de encapsulacioacuten y un perfil de liberacioacuten de corta duracioacuten con
un estallido inicial muy alto De forma similar las nanopartiacuteculas de mezcla PLGA-poloxaacutemero
se modificaron superficialmente introduciendo quitosano en el segundo paso de la siacutentesis Este
meacutetodo mostroacute un perfil de liberacioacuten sostenida de hasta 14 diacuteas sin ninguacuten estallido importante
inicial En este caso se utilizoacute un antiacutegeno recombinante de hepatitis B (Paolicelli et al 2010)
Ademaacutes el uso de heparina conjugada con microesferas porosas de PLGA tambieacuten se ha descrito
para obtener un sistema de administracioacuten a largo plazo que reduce al mismo tiempo el estallido
inicial En estos sistemas la heparina se inmovilizoacute en la superficie nano micropartiacutecula La
liberacioacuten se controloacute usando las afinidades de unioacuten de la heparina a varios factores de
crecimiento incluida la BMP2 En este caso el estallido inicial se redujo hasta un 4-7 durante
el primer diacutea seguido de una liberacioacuten sostenida de aproximadamente un 1 por diacutea (La et al
2010) (Chung et al 2007) (Jeon et al 2008)
La liberacioacuten de proteiacutena en la descarga inicial puede atenuarse mediante la fabricacioacuten de
microesferas de doble pared es decir micropartiacuteculas con nuacutecleo y concha La presencia de un
revestimiento o armazoacuten de PLA reduce la tasa de liberacioacuten de BSA encapsulado en el nuacutecleo
PLGA y extiende la duracioacuten del perfil de liberacioacuten hasta dos meses Por otra parte un aumento
en el peso molecular de PLA influye en la tasa de erosioacuten de las partiacuteculas lo que ralentiza auacuten
maacutes la liberacioacuten de proteiacutenas (Xia et al 2013)
La modificacioacuten de la viscosidad en el entorno de micropartiacuteculas influye adicionalmente en
el patroacuten de liberacioacuten La viscosidad puede controlar el estallido en el punto maacutes temprano y
promover una liberacioacuten sostenida Esta situacioacuten se ha demostrado para las microesferas de
rhBMP2-PLGA incrustadas en un hidrogel de aacutecido quitosano-tioglicoacutelico (Poloxaacutemero 407) (Fu
50
et al 2012) Yilgor y colaboradores tambieacuten incorporaron las nanopartiacuteculas de su sistema de
administracioacuten secuencial a un scaffolds compuesto por quitosano y quitosano-PEO (Yilgor et al
2009) En otro trabajo las microesferas de PLGA PVA con BMP2 encapsulado se combinaron
con diferentes biomateriales compuestos (hidrogel de gelatina o fumarato de polipropileno) La
liberacioacuten sostenida de la moleacutecula bioactiva se extendioacute durante un periacuteodo de 42 diacuteas Los
resultados in vivo indican la importancia de las caracteriacutesticas compuestas En este caso se obtuvo
una mejor formacioacuten de hueso cuando las micropartiacuteculas de PLGA se incorporaron a la matriz
maacutes hidroacutefoba (fumarato de polipropileno) (Kempen et al 2008) (Kempen et al 2009)
Finalmente la tabla 1 resume informacioacuten importante sobre diferentes paraacutemetros relacionados
con el uso de nano o micropartiacuteculas basadas en PLGA para encapsular transportar y liberar
factores de crecimiento (principalmente BMP2) La mayoriacutea de ellos estaacuten en la escala
microscoacutepica El PVA ha sido el estabilizador de surfactante maacutes utilizado Es posible encontrar
tanto la encapsulacioacuten como la adsorcioacuten de superficie de los factores de crecimiento con una
eficiencia alta a moderada El uso de heparina como estabilizador reduce significativamente la
liberacioacuten inicial en estallido favoreciendo una liberacioacuten sostenida en el tiempo La bioactividad
del GF se conservoacute en la mayoriacutea de los sistemas y la encapsulacioacuten con otras biomoleacuteculas parece
tener un efecto similar al del uso de surfactantes como estabilizadores
51
TABLA 1 Sistemas de nano micropartiacuteculas para encapsular GF principalmente el factor
de crecimiento BMP2
Poliacutemeros Estabilizadores Tamantildeo EE
Encapsulacioacuten Liberacioacuten
Actividad
bioloacutegica Referencia
PLGA PVA 10-20 microm Adsorcioacuten
rhBMP2
20 ngml de
contasnte
liberacioacuten
sostenida
Mejor formacioacuten
oacutesea 8 semanas
despueacutes
Fu at al 2012
(126)
PLGA PVA 10-100
μm
rhBMP2-BSA
69 (BMP)
Burst (20 )
Prolongado
hasta un 77
(28 diacuteas)
Moleacuteculas de
BMP2 con
bioactividad
Tian et al
2012
(130)
PLGA 7525 PVA 182 μm 82 -
Buenos resultados
de reparacioacuten oacutesea
de 8 a 12 semanas
Rodriguez-
Evora et al
2014 (130)
PLGA PVA 228 μm 605
30 en la
descarga inicial
Liberacioacuten maacutes
lenta de un 4
por semana
Despueacutes de 8
semanas
liberado un
60
Sin peacuterdida de
bioactividad
Reyes et al
2013 (132)
PLGAPEG Sin siacutentesis de
doble emulsion
100-200
μm Adsorcioacuten BMP2
13 en la
descarga inicial
Liberacioacuten mas
lenta de 001-8
por dia
Despueacutes de 23
diacuteas liberado
un 70
Sustancial
regeneracioacuten oacutesea
debido al
ldquoandamiordquo
Rahman et al
2014 (181)
PLGA Diferente PVA 20-100
μm
30 (sin cubrir
PLGA)
90 (cubriendo
PLGA)
26-49 (1 dia)
Total 2 semanas
despueacutes
Sin peacuterdida de
bioactividad
Lupu-Haber
et al 2013
(134)
PLGA 7525 PVA 5-125 μm -
Descarga inicial
de un 30 (1
diacutea)
Prolongada 35
diacuteas
Mayores
voluacutemenes y
cobertura de
superficie del
Nuevo hueso
Wink et al
2014
(138)
PLGA Heparina 200-800
nm
Adsorcioacuten BMP2
94
Sin descarga
inicial
Prolongado 4
semanas
Reduccioacuten
significativa de la
dosis de BMP2
para una Buena
formacioacuten oacutesea
La et al 2010
(122)
PLGA Heparina-
poloxaacutemero 160 nm
Adsorcioacuten BMP2
100
Descarga inicial
(4-7) perfil
lineal
Mayor
mineralizacioacuten de
la matriz del hueso
regenerado
Chung et al
2007 (123)
PLGA Heparina 100-250
nm Adsorcioacuten 94
Descarga inicial
10 (1 dia)
60 30 diacuteas
despueacutes
Sin peacuterdida de
bioactividad
Eficacia de la
administracioacuten
cantidad 50 veces
menor
Jeon et al
2008 (124)
PLGA PVA ~ 300 nm 80 85 descarga
inicial (1 dia)
Sin peacuterdida de
bioactividad
Yilgor et al
2009 (127)
PLGA (en anillos) PVA 215 μm 66 Descarga El 60 de los Rodriguez-
52
15 Vectorizacioacuten Entrega dirigida
Las nano-esferas de PLGA representan un sistema de administracioacuten de biomoleacuteculas bien
estudiado que podriacutea aplicarse a la seleccioacuten celular con el fin de mejorar el suministro de
proteiacutenas especiacuteficas o aacutecidos nucleicos dentro o cerca de las ceacutelulas de referencia de ingenieriacutea
oacutesea es decir ceacutelulas madre mesenquimales (Vo Kasper and Mikos 2012) Las propiedades de
direccionamiento pueden ser suministradas por una estrategia de funcionalizacioacuten del ligando
modificacioacuten de la estructura superficial del nano-transportador conjugando un ligando especiacutefico
de ceacutelula para dirigir la liberacioacuten de biomoleacuteculas encapsuladas preferiblemente en estrecha
asociacioacuten con las ceacutelulas diana (Ji et al 2012) El uso de nanopartiacuteculas con una unioacuten covalente
de diferentes ligandos da lugar a una teacutecnica potencial para administrar biomoleacuteculas especiacuteficas
de ceacutelulas oacuteseas para la ingenieriacutea oacutesea (Luginbuehl et al 2004)
Los anticuerpos especiacuteficos que reconocen los receptores de superficie en estas ceacutelulas podriacutean
acoplarse covalentemente a la superficie de las nanopartiacuteculas de PLGA obteniendo inmuno-
nanopartiacuteculas Hay varios ejemplos de inmovilizacioacuten de anticuerpos en la superficie de
nanopartiacuteculas de PLGA Kocbek y colaboradores demostraron el reconocimiento especiacutefico de
las ceacutelulas tumorales de mama por un anticuerpo monoclonal especiacutefico unido a las nanopartiacuteculas
fluorescentes PLGA obtenidas mediante el proceso de emulsioacuten WOW (Kocbek et al 2007)
Para la unioacuten covalente de la superficie utilizaron un meacutetodo de carbodimida maacutes simple que
promueve la formacioacuten de un enlace amida entre los grupos terminales carboxiacutelicos libres de
nanopartiacuteculas de PLGA y los grupos amino primarios de la moleacutecula del anticuerpo (Ertl et al
2000) Este procedimiento puede verse muy influenciado por la presencia de estabilizadores
frecuentemente utilizados para conferir estabilidad coloidal a las nanopartiacuteculas La movilidad
electroforeacutetica de las nanopartiacuteculas de PLGA con un anticuerpo (inmuno-γ-globulina anti-
proteiacutena C-reactiva humana) unido covalentemente a la superficie como se muestra en la figura
5 Es necesario observar la draacutestica disminucioacuten en los valores de movilidad del anticuerpo
modificado con respecto a las nanopartiacuteculas de PLGA desnudas lo que podriacutea implicar una baja
53
estabilidad coloidal y la posterior agregacioacuten del nanosistema Santander y colaboradores
propusieron una menor carga de anticuerpos en la que las nanoparticulas de PLGA desnudas
deben ser recubiertas por un agente surfactante no ioacutenico con el fin de obtener nanopartiacuteculas
estables inmunorrectivas (Santander-Ortega Bastos-Gonzalez and Ortega-Vinuesa 2007)
Ratzinger y colaboradores indicaron que la presencia de altas concentraciones de poloxaacutemero
disminuyoacute la eficacia de acoplamiento a grupos carboxiacutelicos en nanopartiacuteculas de PLGA lo que
demuestra que es necesario un equilibrio que combine maacutes estabilidad y mejor eficacia de
acoplamiento (Ratzinger et al 2010) Para evitar este problema Cheng y colaboradores
sintetizaron el copoliacutemero en bloque de PLGA-PEG funcionalizado con carboxilo uniendo un
aptaacutemero especiacutefico a la superficie de las nanopartiacuteculas pegiladas mediante el meacutetodo de la
carbodiimida En este trabajo se ha demostrado una mejor administracioacuten de faacutermacos en los
tumores de proacutestata en comparacioacuten con las nanopartiacuteculas equivalentes no dirigidas (Cheng et
al 2007)
16 Ingenieriacutea tisular soportes 3D o ldquoscaffoldsrdquo
Los datos publicados en la literatura indican que las nanopartiacuteculas micropartiacuteculas de PLGA
prometen lograr una administracioacuten sostenida espacial y temporalmente controlada por factores
de crecimiento requeridos para el desarrollo celular y la diferenciacioacuten celular Se pueden
incorporar a ceacutelulas en scaffolds soacutelidos o hidrogeles inyectables (Danhier et al 2012) Los
scaffolds o andamios son estructuras 3D porosas que normalmente se utilizan para mejorar la
ingenieriacutea tisular oacutesea (A C Carreira et al 2014) De acuerdo con Tian y colaboradores (Tian et
al 2012) un scaffolds disentildeado con este objetivo debe tener (1) resistencia mecaacutenica apropiada
para soportar el crecimiento de hueso nuevo (2) porosidad apropiada para permitir el crecimiento
de las ceacutelulas relacionadas con los huesos (3) buena biocompatibilidad que permite el crecimiento
de ceacutelulas en su superficie sin ser rechazado por el cuerpo (4) baja toxicidad para las ceacutelulas y
tejidos de alrededor (5) ser capaz de inducir la diferenciacioacuten osteogeacutenica de las ceacutelulas madre
54
relacionadas con los huesos (6) ser biodegradable con productos de degradacioacuten no toacutexicos para
que eventualmente puedan ser reemplazados por nuevo hueso Ademaacutes el scaffolds para la
regeneracioacuten oacutesea debe mantener el suministro o la liberacioacuten de BMP (factores de crecimiento)
in situ durante un tiempo prolongado De esta forma las nano micropartiacuteculas dentro de los
scaffolds se utilizan para liberar un flujo adecuado de estas biomoleacuteculas de sentildealizacioacuten y
preservar su estructura funcional (Romagnoli DrsquoAsta and Brandi 2013) Para hacerlo se requiere
una liberacioacuten inicial del factor de crecimiento encapsulado en las primeras horas para poder
obtener raacutepidamente una concentracioacuten terapeacuteutica efectiva seguida de un perfil de liberacioacuten
sostenido a largo plazo (Puppi et al 2014) La mayoriacutea de las partiacuteculas polimeacutericas insertadas
en las estructuras de los scaffolds estaacuten en una escala micromeacutetrica El objetivo principal de estas
micropartiacuteculas es la proteccioacuten y el control temporal de la entrega del factor de crecimiento Sin
embargo dada la porosidad de estas estructuras las nanopartiacuteculas y especialmente las partiacuteculas
de algunas micras pueden volverse maacutes importantes ya que es posible disentildear sistemas con una
difusioacuten simple y faacutecil a traveacutes de la estructura Este proceso podriacutea permitir el reconocimiento
especiacutefico de un tipo de ceacutelula particular liberando sus BMP encapsuladas en el mismo entorno
y ayudando a su diferenciacioacuten al tejido celular oacuteseo
55
2 HIPOacuteTESIS
Lo que sabemos hasta ahora es que las BMPs y especiacuteficamente la BMP-2 son muy uacutetiles
para promover la regeneracioacuten oacutesea induciendo una mayor formacioacuten de hueso de la zona
receptora de igual calidad que el hueso nativo del paciente Sin embargo la administracioacuten local
presenta algunas limitaciones como que la proteiacutena se puede inactivar muy raacutepidamente y la
distribucioacuten de la BMP en una suspensioacuten liacutequida hace que sea imposible estar seguro de que la
proteiacutena haya alcanzado el objetivo Para ayudar a resolver estos problemas planteamos la
siguiente hipoacutetesis
HIPOTESIS CIERTA La encapsulacioacuten de BMP-2 con nuestras nanopartiacuteculas permiten una
administracioacuten localizada un transporte especifico y una liberacioacuten controlada de la biomoleacutecula
mejorando asiacute su farmacocineacutetica y farmacodinamia y disminuyendo los efectos secundarios
derivados de esta
HIPOacuteTESIS NULA Que tras la experimentacioacuten no seamos capaces de promover la
encapsulacioacuten de la BMP-2 dentro de nuestras nanopartiacuteculas de PLGA o que producieacutendose no
se produzca la administracioacuten de BMP-2 localizada y liberacioacuten prolongada o que nuestro sistema
de encapsulacioacuten pueda presentar efectos citotoacutexicos a nivel celular
56
3OBJETIVOS
31 Objetivo principal
Optimizar la formulacioacuten de diferentes tipos de nanopartiacuteculas de PLGA para el transporte y
suministro de BMP-2 que permita conseguir una cineacutetica de liberacioacuten controlada aumentando la
vida media de este factor de crecimiento oacuteseo y preservando su accioacuten bioloacutegica
32 Objetivos secundarios
Nos planteamos los siguientes
1 Llevar a cabo la siacutentesis de NPs polimeacutericas de PLGA mediante el procedimiento de
doble emulsioacuten para conseguir nanosistemas coloidal y temporalmente estables
2 Modelar los procedimientos de siacutentesis de NPs de PLGA para la encapsulacioacuten o carga
de moleacuteculas proteicas en la proporcioacuten adecuada sin alterar su actividad bioloacutegica
3 Desarrollar una completa caracterizacioacuten quiacutemico-fiacutesica de los diferentes nanosistemas
polimeacutericos en ausencia de proteiacutena y con carga de la misma haciendo hincapieacute en la
interaccioacuten surfactante-proteiacutena y analizando sus propiedades superficiales y
coloidales
4 Desarrollar una caracterizacioacuten bioloacutegica de los diferentes nanosistemas con proteiacutena
centrada en el anaacutelisis de la cineacutetica de liberacioacuten proteica y su actividad bioloacutegica
5 Evaluar in vitro las interacciones celulares citotoxicidad y captacioacuten celular de los
diferentes sistemas de NPs de PLGA en ceacutelulas estromales mesenquimales de hueso
alveolar humano
6 Tomando como punto de partida el modelo de NPs con proteiacutena con las mejores
propiedades coloidales y bioloacutegicas formular las condiciones oacuteptimas para obtener un
nanotransportador de partiacuteculas de PLGA cargado con BMP2
57
7 Desarrollar una completa caracterizacioacuten quiacutemico-fiacutesica y bioloacutegica de las NPs con
BMP2 analizando sus propiedades superficiales coloidales y la cineacutetica de liberacioacuten
proteica
8 Analizar la actividad bioloacutegica de la BMP-2 vehiculizada mediante las NPs de PLGA
mediante experiencias de proliferacioacuten migracioacuten y diferenciacioacuten osteogeacutenica en
ceacutelulas estromales mesenquimales de hueso alveolar humano
58
4NANOSISTEMA DE ENTREGA DOBLE PARA BIOMOLEacuteCULAS
FORMULACIOacuteN CARACTERIZACIOacuteN Y LIBERACIOacuteN IN
VITRO
41Antecedentes
La regeneracioacuten tisular es una accioacuten bioloacutegica compleja que implica muacuteltiples pasos de forma
secuencial ordenada y controlada (Padial-Molina et al 2012) (Padial-Molina Rodriguez et al
2015) Claacutesicamente se han propuesto moleacuteculas bioactivas para ayudar en estos procesos Sin
embargo el uso de altas dosis la desnaturalizacioacuten y la peacuterdida de actividad bioloacutegica el tiempo
de accioacuten descontrolado y la difusioacuten a otros tejidos destacan como los principales problemas de
esta estrategia terapeacuteutica (Ortega-Oller et al 2015) Para ayudar a resolver estas dificultades en
los uacuteltimos antildeos se ha investigado intensamente la nanomedicina como un aacuterea emergente Esto
implica meacutetodos de diagnoacutestico terapeacuteuticos y de regeneracioacuten mediante estructuras y sistemas
en los que el tamantildeo y la forma se controlan a nivel atoacutemico molecular y supramolecular (Ki-
Bum Lee Ani Solanki J Dongun Kim 2009) El transporte y la administracioacuten controlada de
faacutermacos yo biomoleacuteculas terapeacuteuticas mejora su farmacocineacutetica y farmacodinaacutemica y al
mismo tiempo minimiza los efectos secundarios nocivos Para estos propoacutesitos se describieron
diferentes tipos de nanosistemas El aacutecido polilaacutectico-co-glicoacutelico (PLGA) exhibe una baja
citotoxicidad asi como una alta biocompatibilidad y biodegradabilidad con las liberaciones de
subproductos no toacutexicos
En la uacuteltima deacutecada se ha investigado el uso de PLGA para administrar un amplio espectro de
agentes activos desde moleacuteculas de faacutermacos hidroacutefobas (Yallapu et al 2010) (Nair and Sharma
2012) (Shankarayan Kumar and Mishra 2013) a biomoleacuteculas hidroacutefilas como peacuteptidos
(Loureiro et al 2016) proteiacutenas (Blanco and Alonso 1998) (Perez De Jesus and Griebenow
2002) (Manuel J Santander-Ortega Csaba et al 2010) (Pirooznia et al 2012) (drsquoAngelo et al
2010) (White et al 2013) o aacutecidos nucleicos (Pantazis et al 2012) (Park et al 2013) Estos
59
sistemas de entrega se han producido a traveacutes de diferentes procesos de formulacioacuten para su
aplicacioacuten en terapias tanto sisteacutemicas como locales especiacuteficas del sitio (Wan and Yang 2016)
Sin embargo su disentildeo y desarrollo como nanotransportadores son difiacuteciles debido al patroacuten
de liberacioacuten problemaacutetico que presentan cuando las moleacuteculas encapsuladas son proteiacutenas para
las cuales las descargas iniciales y la liberacioacuten lenta o incompleta podriacutean ser un problema (Wan
and Yang 2016) (Giteau et al 2008) (Fredenberg et al 2011) Ademaacutes las condiciones
especiacuteficas de la liberacioacuten pueden necesitar ser diferentes dependiendo de la aplicacioacuten final del
nanotransportador (Fredenberg et al 2011) (Mohamed and van der Walle 2008)
La teacutecnica de doble emulsioacuten de aguaaceiteagua (WOW) es el meacutetodo de encapsulacioacuten de
proteiacutenas maacutes ampliamente utilizado para PLGA en micro (MP) y nanopartiacuteculas (NP) (Csaba et
al 2004) (Makadia and Siegel 2011) Permite modular diferentes factores como el tipo de PLGA
el uso de otros poliacutemeros mezclados con PLGA la adicioacuten de surfactantes el estreacutes mecaacutenico o
el solvente orgaacutenico (Fredenberg et al 2011) Tambieacuten es posible construir varios tipos de
copoliacutemeros para modificar la hidrofobicidad la relacioacuten de hidrofilicidad (Wan and Yang 2016)
(Danhier et al 2012) y la estabilidad el tamantildeo y el proceso de liberacioacuten coloidal El par PLGA
polietilenglicol y los surfactantes como el alcohol poliviniacutelico (PVA) o los oacutexidos de polietileno
(PEO) son los maacutes ampliamente estudiados (Nair and Sharma 2012) (Manuel J Santander-
Ortega Csaba et al 2010) (Ratzinger et al 2010) (Meng et al 2003)
Por otro lado la ingenieriacutea de tejidos requiere la participacioacuten de ceacutelulas estromales
mesenquimales (MSC) (Padial-Molina OrsquoValle et al 2015) Se sabe que las MSC tienen la
capacidad de diferenciarse en muacuteltiples tipos de ceacutelulas incluidos los osteoblastos Los
osteoblastos son las ceacutelulas principales responsables de sintetizar el tejido oacuteseo mineralizado Este
proceso estaacute regulado por entre otras moleacuteculas BMP-2 (Ortega-Oller et al 2015) Las
partiacuteculas de PLGA cargadas con BMP-2 son sistemas ampliamente utilizados como se ha
descrito y revisado por otros autores (Ortega-Oller et al 2015) (Yilgor Hasirci and Hasirci 2010)
(Li et al 2009) (Wang et al 2015) (Shim et al 2016)
60
Asiacute dentro de este contexto el objetivo del presente estudio fue optimizar la formulacioacuten y
propiedades de un sistema de nanopartiacuteculas con gran variedad de aplicaciones terapeacuteuticas
Probamos dos estrategias diferentes para obtener NP de tensioactivo PLGA utilizando lisozima
como modelo para BMP-2 Analizamos el tamantildeo y la morfologiacutea el iacutendice de polidespersidad
el potencial zeta la estabilidad coloidal y la eficiencia de encapsulacioacuten (EE) de la proteiacutena
Una vez finalizada la caracterizacioacuten fiacutesico-quiacutemica el estudio se centroacute en el proceso de
liberacioacuten de proteiacutenas utilizando diferentes teacutecnicas para estudiar los resultados de experimentos
in vitro y centraacutendose en el patroacuten de liberacioacuten y la actividad bioloacutegica de la lisozima liberada
De esta manera se establecioacute una nueva formulacioacuten para desarrollar un nanosistema de PLGA
con una distribucioacuten de tamantildeo dual singular y el equilibrio adecuado entre encapsulacioacuten y
liberacioacuten de proteiacutenas bioloacutegicamente activas Finalmente los efectos del sistema PLGA
propuesto se probaron en MSC primarias in vitro como prueba del nuevo sistema desarrollado
42Materiales y meacutetodos
421Formulacioacuten de las nanoparticulas
El aacutecido poli (laacutectico-co-glicoacutelico) (PLGA 5050) ([C2 H2 O2]x [C3 H4 O2]y) x = 50 y = 50
(Resomerreg 503H) 32 - 44 kDa se usoacute como poliacutemero El surfactante polimeacuterico Pluronicreg F68
(Poloxaacutemero 188) (Sigma-Aldrich) se usoacute como el emulsionante Su estructura se basa en un
copoliacutemero tri-bloque de poli (oacutexido de etileno) PEO y poli (oacutexido de propileno) PPO (PEO)a ndash
(PPO)b ndash (PEO)a con a = 75 y b = 30 La lisozima de huevo de gallina (Sigma-L7651) se usoacute
como proteiacutena hidroacutefila El agua se purificoacute en un sistema Milli-Q Academic de Millipore Se
desarrollaron dos meacutetodos de formulacioacuten diferentes denominados O-F68 y W-F68
En el meacutetodo O-F68 se disolvieron 25 mg de PLGA y 15 mg de F68 en 660 120583119871 de
diclorometano (DMC) y se agitaron en el vortex Luego se antildeadieron 330 micro-litros de acetona
y se agitaron en el vortex tambieacuten A continuacioacuten se antildeadieron 100 120583119871 de una solucioacuten
tamponada a pH 128 con o sin lisozima (5 mg mL) gota a gota mientras se agita en vortex
61
durante 30 s Inmediatamente esta emulsioacuten primaria de agua aceite (WO) se vertioacute en un vidrio
que conteniacutea 125 ml de etanol bajo agitacioacuten magneacutetica y se antildeadieron 125 ml de agua MilliQ
Despueacutes de 10 minutos de agitacioacuten magneacutetica los disolventes orgaacutenicos se extrajeron
raacutepidamente por evaporacioacuten al vaciacuteo hasta que la muestra alcanzoacute un volumen final de 10 ml
En el meacutetodo W-F68 se disolvieron 100 mg de PLGA en un tubo que conteniacutea 1 ml de acetato
de etilo (EA) y se agitaron en vortex Se antildeadieron 40 120583119871 de una solucioacuten tamponada a pH 128
con o sin lisozima (20 mg ml) e inmediatamente se sonicaron (Branson Ultrasonics 450 Analog
Sonifier) fijando el debido ciclo de trabajo al 20 y de control de salida a 4 durante 1 min con
el tubo rodeado de hielo Esta emulsioacuten primaria WO se vertioacute en un tubo de plaacutestico que conteniacutea
2 ml de una solucioacuten tamponada (pH 128) de F68 a 1 mg ml y se agitoacute en voacutertex durante 30 s
Luego el tubo rodeado de hielo se sonicoacute de nuevo a la maacutexima amplitud para la micro punta
(control de salida 7) durante 1 minuto Esta segunda emulsioacuten WOW se vertioacute en un vaso que
conteniacutea 10 ml de la solucioacuten tamponada de F68 y se mantuvo bajo agitacioacuten magneacutetica durante
2 min El disolvente orgaacutenico se extrajo luego raacutepidamente por evaporacioacuten al vaciacuteo hasta un
volumen final de 8 ml
422 Limpieza y almacenamiento
Despueacutes de la evaporacioacuten del disolvente orgaacutenico la muestra se centrifugoacute durante 10 min a
20 deg C a 14000 o 12000 rpm para los meacutetodos O-F68 y W-F68 respectivamente El sobrenadante
se filtroacute usando filtros de 100 nm para medir la proteiacutena no encapsulada libre El sedimento se
resuspendioacute luego en PB hasta un volumen final de 4 ml y se mantuvo en refrigeracioacuten a 4ordmC
1La carga de proteiacutenas y la eficiencia de encapsulacioacuten
La carga de proteiacutena inicial se optimizoacute para la formulacioacuten de nanopartiacuteculas preservando la
estabilidad coloidal final despueacutes del paso de evaporacioacuten y siendo diferente para cada
nanosistema Ademaacutes se usoacute 16 p p (Lys PLGA) para O-F68 y 08 p p (Lys PLGA)
para W-F68 La cantidad de lisozima encapsulada se calculoacute midiendo la diferencia entre la
cantidad inicial antildeadida y la proteiacutena libre no encapsulada que se analizoacute mediante el ensayo con
62
aacutecido bicinconiacutenico (BCA Sigma-Aldrich) Luego la eficacia de encapsulacioacuten de proteiacutenas (EE)
y la carga de faacutermaco final (DL) se calcularon de la siguiente manera
EE = 119872119868minus119872119865
119872119868119909 100 119863119871 =
119872119868minus119872119865
119872119868119901119900119897119894119898119890119903 119909 10
donde MI es la masa total inicial de Lys MF es la masa total de Lys en el sobrenadante acuoso
y Mpoliacutemero es la masa de PLGA en la formulacioacuten
423 Caracterizacioacuten de las nanoparticulas
2Caracterizacioacuten interfacial de la primera emulsioacuten agua en aceite
La tensioacuten superficial y las mediciones de reologiacutea dilatacional en la interfaz aire-agua se
realizaron en el OCTOPUS (Maldonado-Valderrama et al 2013) dispositivo de anaacutelisis de
superficies por gota pendiente con intercambio muacuteltiple de subfase (patente presentada
P201001588) descrito en detalle por Cabrerizo-Viacutelchez y col (Wege Holgado-Terriza and
Cabrerizo-Vilchez 2002) Aquiacute el aire juega el papel de la fase orgaacutenica La tensioacuten superficial
se calcula con el software DINATENreg basado en el anaacutelisis de forma de gota axisimeacutetrica
(ADSA) y el moacutedulo de dilatacioacuten (E) de la capa interfacial se determina a partir del anaacutelisis de
imagen con el programa CONTACTOreg
3Morfologiacutea de la partiacutecula
Las nanopartiacuteculas se obtuvieron por microscopiacutea electroacutenica de barrido (SEM) y microscopiacutea
electroacutenica de transmisioacuten de barrido (STEM) usando un microscopio electroacutenico de barrido de
emisioacuten de campo Zeiss SUPRA 40VP del Centro de Instrumentacioacuten Cientiacutefica de la Universidad
de Granada (CIC UGR)
4Tamantildeo de las nanoparticulas y movilidad electrocineacutetica
El diaacutemetro hidrodinaacutemico y la movilidad electroforeacutetica de las NP se determinaron usando un
dispositivo Zetasizer NanoZeta ZS (Malvern Instrument Ltd UK) que trabaja a 25 deg C con un
63
laacuteser He-Ne de 633 nm y un aacutengulo de dispersioacuten de 173 deg Cada punto de datos se tomoacute como un
promedio de tres mediciones de muestra independientes El tamantildeo de las NP se caracterizoacute por
escaacutener de luz dinaacutemica (DLS) Se calcularon el diaacutemetro hidrodinaacutemico promedio (media Z o
media acumulada) y el iacutendice de polidispersidad (PDI) Estos paraacutemetros se calculan a traveacutes de
un anaacutelisis acumulativo de los datos que es aplicable para las distribuciones de tamantildeo
monomodal estrecho (Hassan Rana and Verma 2015) Tambieacuten determinamos la distribucioacuten
del tamantildeo de intensidad a partir de un algoritmo proporcionado por el software Zetasizer
La movilidad electroforeacutetica se determinoacute mediante la teacutecnica de electroforesis laacuteser Doppler
Se establecioacute una distribucioacuten de movilidad electroforeacutetica asiacute como una movilidad
electroforeacutetica promedio para cada muestra (entendiendo por promedio dos veces seguidas para
cada una de las muestras)
La distribucioacuten del tamantildeo hidrodinaacutemico de las NP con distribuciones de tamantildeo amplio de
DLS tambieacuten se midioacute usando anaacutelisis de seguimiento de nanopartiacuteculas (NTA) en un NanoSight
LM10-HS (GB) FT14 (NanoSight Amesbury Reino Unido) Todas las muestras se midieron maacutes
de tres veces durante 60 s con ajuste manual del obturador ganancia brillo y umbral a 25 deg C La
distribucioacuten de tamantildeo promedio (concentracioacuten de partiacuteculas frente a diaacutemetro) se calculoacute como
un promedio de al menos tres independientes distribuciones del tamantildeo
5Resonancia magneacutetica nuclear (RMN) de las nanopartiacuteculas
El espectro de 1HNMR de F68 libre las partiacuteculas cargadas con lisozima del meacutetodo O-F68
con y sin F68 y las partiacuteculas cargadas con lisozima del meacutetodo W-F68 se midieron con un
espectroacutemetro VNMRS de 500 MHz (Agilent) en el Centro de Instrumentacioacuten Cientiacutefica (CIC)
de la Universidad de Granada
424 Estabilidad coloidal y temporal en biologiacutea media
Se midioacute el diaacutemetro hidrodinaacutemico promedio y el iacutendice de polidispersidad (PDI) por DLS de
cada sistema para determinar su estabilidad coloidal en diferentes medios (tampoacuten de fosfato
64
[PB] solucioacuten salina tamponada con fosfato [PBS] y medio de cultivo celular medio de Eagle
modificado de Dulbecco [DMEM] de Sigma) y en diferentes momentos despueacutes (0 1 y 5 diacuteas)
Los experimentos de liberacioacuten in vitro se realizaron siguiendo una metodologiacutea similar a la
descrita anteriormente (eficacia de encapsulacioacuten) pero utilizando 1 ml de cada muestra
suspendida en PBS a 37 C La proteiacutena liberada de estas muestras se determinoacute cada 24 horas
mediante anaacutelisis de sobrenadante y el sedimento se suspendioacute en el mismo volumen de tampoacuten
para mantener las condiciones de liberacioacuten Todos los experimentos fueron desarrollados por
triplicado
6Microscopia confocal
La lisozima se marcoacute con isotopo de fluoresceiacutena (FITC) usando un meacutetodo descrito por Kok
et al (Kok et al 1998) Despueacutes de la conjugacioacuten covalente de FITC y lisozima las
concentraciones se estimaron espectrofotomeacutetricamente utilizando los coeficientes de extincioacuten
descritos para FITC a 494 nm y 280 nm La concentracioacuten de lisozima se calculoacute midiendo la
absorbancia oacuteptica a 280 nm y restando la absorbancia FITC correspondiente a esta longitud de
onda Las imaacutegenes se realizaron en un microscopio confocal de escaneo laacuteser Nikon A1 de CIC
UGR Todos los experimentos se realizaron por triplicado y se replicaron al menos dos veces
425 Actividad bioloacutegica e interacciones
7Actividad bioloacutegica de la lisozima
La actividad bioloacutegica de la lisozima se analizoacute mediante un kit de actividad enzimaacutetica
(Sigma-Aldrich) utilizando ceacutelulas de Micrococcus lysodeikticus como sustrato siguiendo las
instrucciones del fabricante
8Captacioacuten celular
Se tomaron ceacutelulas madre mesenquimales humanas primarias (hMSC) del hueso alveolar
maxilar sano de acuerdo con protocolos descritos (Mason et al 2014) Despueacutes de confirmar su
fenotipo por pruebas de citometriacutea de flujo y diferenciacioacuten trilinaje 12000 ceacutelulas por pozo se
cultivaron en placas esteacuteriles con fondo de vidrio (Ibidi cat n 81158) durante la noche Estas
65
ceacutelulas fueron tratadas con medio sin suero fetal bovino (FBS) y guiacutea celular roja (1 5000)
(C34552 ThermoFisher) durante 30 min Entonces el medio fue eliminado y complementado con
10 de SFB despueacutes de lo cual se agregaron partiacuteculas con lisozima-FITC Entonces los hMSCs
eran incubadas 30 minutos nuevamente lavadas tres veces con PBS 1X y un suplementado medio
fresco con 2 de FBS agregado Finalmente las hMSCs fueron examinadas por un microscopio
confocal (Nikon Eclipse Ti-E) Cultivo celular en todos los casos se mantuvieron a 37 deg C y 5
de atmoacutesfera de CO
43 Resultados y discusioacuten
431 Formulacioacuten de las nanoparticulas
Los meacutetodos desarrollados en este trabajo estaacuten destinados a mejorar las teacutecnicas de
formulacioacuten existentes para las NP de PLGA cargadas de proteiacutenas hidrofiacutelicas basadas en un
proceso de doble emulsioacuten (Blanco and Alonso 1998) (Csaba et al 2004) La novedad de estos
meacutetodos es el uso del surfactante polimeacuterico F68 ya sea en la fase orgaacutenica (meacutetodo O-F68) o en
la fase acuosa (W-F68) Este surfactante reduce el tamantildeo de las NP mejora su estabilidad y
protege la proteiacutena encapsulada Ademaacutes la presencia de F68 en la superficie de las partiacuteculas
reduce el reconocimiento de los nanovehiacuteculos (nanotransportadores) por el sistema mononuclear
fagociacutetico (MPS) (Farace et al 2016)
Ademaacutes la eleccioacuten del solvente orgaacutenico afecta significativamente las propiedades del
sistema coloidal final ya que la solubilidad del solvente orgaacutenico regula la estructura interna y
superficial de la partiacutecula Ademaacutes la interaccioacuten del disolvente con la biomoleacutecula encapsulada
puede alterar su bioactividad como consecuencia de su desnaturalizacioacuten como se encontroacute para
el cloruro de metileno (Meng et al 2003) En el meacutetodo O-F68 se elige DMC como disolvente
orgaacutenico debido a su menor solubilidad en agua para facilitar el proceso de emulsificacioacuten y su
bajo punto de ebullicioacuten para facilitar la evaporacioacuten Sin embargo se antildeadioacute un solvente orgaacutenico
libremente miscible en agua (acetona) y el emulsionante F68 en esta fase orgaacutenica para reducir
66
sus efectos bioloacutegicos negativos sobre la proteiacutena encapsulada (Danhier et al 2012) Este
emulsionante tambieacuten reduce la interaccioacuten de la matriz de PLGA proteiacutena-hidrofoacutebica y por lo
tanto la interrupcioacuten de la estructura de la proteiacutena (Ortega-Oller et al 2015) Por el contrario
en el meacutetodo W-F68 se utilizoacute acetato de etilo como disolvente orgaacutenico que ejerce menos
efectos de desnaturalizacioacuten sobre la proteiacutena encapsulada (Sturesson and Carlfors 2000) La
mayor solubilidad en agua de este solvente favorece la eliminacioacuten raacutepida del solvente La
velocidad de eliminacioacuten del disolvente tambieacuten se acelera al aumentar la tensioacuten de cizallamiento
durante la segunda etapa de emulsificacioacuten Tambieacuten mejora la eficacia del encapsulamiento y
minimiza el tiempo de contacto entre la proteiacutena y el disolvente orgaacutenico (Ortega-Oller et al
2015) El poloxaacutemero F68 se introduce en la fase acuosa externa
Ambas formulaciones (O-F68 y W-F68) (Tabla 2) dieron lugar a muestras coloidalmente
estables y a la encapsulacioacuten de la lisozima dentro de las nanopartiacuteculas de acuerdo con el doble
meacutetodo de emulsioacuten WOW (Makadia and Siegel 2011)
67
PLGA
(mg)
F68
(mg)
LYSI
(mg)
Initial EE
LYSF
(mg)
DL
O-F68-Lys 25 15 04 16 625 025 1
W-F68-Lys 100 2 08 08 731 058 058
Tabla 2 Condiciones de formulacioacuten y resultados de encapsulacioacuten de proteiacutenas PLGA F68
y LYSI son la cantidad inicial de poliacutemero surfactante y lisozima respectivamente El inicial
es la tasa inicial de proteiacutena-poliacutemero en pesopeso EE es la eficiencia de encapsulacioacuten LYSF
es la cantidad final encapsulada de lisozima DL es la tasa final de carga del faacutermaco en
pesopeso
La lisozima fue elegida como una proteiacutena modelo debido a su bioestabilidad sus
caracteriacutesticas bien conocidas y su facilidad para cuantificar su actividad bioloacutegica (Lin et al
2007) (Cai et al 2008) Ademaacutes su tamantildeo molecular (143 kD) y su punto isoeleacutectrico baacutesico
(alrededor de pH = 11) lo convierten en un modelo apropiado para otras proteiacutenas como los
factores de crecimiento oacuteseo (White et al 2013) Tres objetivos principales impulsaron la
optimizacioacuten de la relacioacuten apropiada entre el poliacutemero el poloxaacutemero y la proteiacutena (1) para
tener nanosistemas coloides estables de tamantildeos submicromeacutetricos (2) encapsular una cantidad
suficiente de proteiacutena y (3) para prevenir la desestabilizacioacuten de proteiacutenas manteniendo su
actividad bioloacutegica
Por lo tanto independientemente del meacutetodo de formulacioacuten se pretendiacutea limitar la carga de
proteiacutena inicial para proporcionar nanosistemas estables coloidalmente En nuestro caso como se
muestra en la Tabla 2 los valores de iniciales fueron la mejor opcioacuten para mantener la
estabilidad coloidal sin cambiar significativamente la distribucioacuten del tamantildeo (ver a
continuacioacuten) En consecuencia DL presenta valores relativamente bajos para ambas
formulaciones aunque la cantidad encapsulada de lisozima LYSF es mayor que las requeridas
68
para proteiacutenas terapeacuteuticas con cantidades cliacutenicamente efectivas maacutes bajas (Paillard-Giteau et
al 2010) El valor de EE encontrado para las NP de O-F68-Lys estaacute en consonancia con las
caracteriacutesticas de la formulacioacuten y es similar a otros informes con diferentes proteiacutenas (Manuel J
Santander-Ortega Csaba et al 2010) (Blanco and Alonso 1998) (Santander-Ortega et al 2009)
(drsquoAngelo et al 2010) incluida la albuacutemina de suero bovino (BSA) o la insulina (Manuel J
Santander-Ortega Csaba et al 2010) (Santander-Ortega et al 2009) y varios factores de
crecimiento (drsquoAngelo et al 2010)
La presencia de surfactante estabiliza la emulsioacuten y reduce su tamantildeo Sin embargo tambieacuten
altera la interaccioacuten proteiacutena-poliacutemero lo que se traduce en una reduccioacuten de la eficacia de
encapsulacioacuten Esto fue evidenciado por Blanco y colaboradores al encapsular BSA y lisozima en
diferentes micropartiacuteculas de PLGA-poloxaacutemero (Blanco and Alonso 1998) Ademaacutes el tipo de
proteiacutena y su carga teoacuterica inicial son factores directamente relacionados con la EE y pueden
afectar la estabilidad coloidal de la emulsioacuten primaria como lo muestran Santander y
colaboradores (Manuel J Santander-Ortega Csaba et al 2010) La diferente relacioacuten poliacutemero
tensioactivo entre las dos formulaciones no es comparable ya que el tensioactivo se agrega de una
manera diferente En ambos casos utilizamos formulaciones anteriores como punto de partida
(Blanco and Alonso 1998) (Csaba et al 2004) y probamos varias relaciones poliacutemero
tensioactivo (datos no mostrados) con el fin de obtener la mejor estabilidad coloidal EE y DL
En la Tabla 2 mostramos los datos para las relaciones optimizadas de PLGA F68 en ambos
sistemas
En el meacutetodo W-F68 a pesar del mayor valor de EE con respecto al sistema O-F68 se esperaba
una encapsulacioacuten casi completa debido a la baja relacioacuten inicial de proteiacutena masa de PLGA
(Manuel J Santander-Ortega Csaba et al 2010) y a la ausencia de surfactante en la primera
emulsioacuten Las caracteriacutesticas del proceso de formulacioacuten modificado pueden tener la clave En
esta formulacioacuten la solubilidad relativamente alta del acetato de etilo en agua promueve la
difusioacuten raacutepida del disolvente orgaacutenico en la segunda fase acuosa Inicialmente se agrega un
69
pequentildeo volumen inicial de agua que contiene poloxaacutemero para evitar una precipitacioacuten raacutepida e
incontrolada del poliacutemero y para controlar la velocidad del proceso Esto se complementa
posteriormente con la adicioacuten de un volumen acuoso maacutes grande como se describioacute anteriormente
(Meng et al 2003) Cuando esta solidificacioacuten es lenta favorece el escape de la proteiacutena y la EE
disminuye Sin embargo si la solidificacioacuten es muy raacutepida el contacto de la proteiacutena con el
solvente orgaacutenico se minimiza y la EE aumenta En el lado negativo puede producir aglomeracioacuten
de poliacutemero que interfiere con la correcta formacioacuten de las NP La introduccioacuten de un paso
intermedio con un volumen reducido de fase acuosa con poloxaacutemero puede modular la velocidad
del proceso controlando la difusioacuten de acetato de etilo en el agua y permitiendo la difusioacuten en la
fase orgaacutenica del poloxaacutemero Una velocidad controlada del proceso de pre-solidificacioacuten del
poliacutemero en presencia de surfactante puede producir canales o poros en la cubierta polimeacuterica
que por un lado podriacutean facilitar la liberacioacuten de proteiacutena y por otro lado podriacutea reducir el valor
EE (Rosca Watari and Uo 2004) Como resultado de estos fenoacutemenos los DL finales (p p de la
lisozima poliacutemero) que se muestran en la Tabla 2 para ambos sistemas NP son adecuados para
su aplicacioacuten como sistemas de nanotransporte
432 Caracterizacioacuten de las Nanopartiacuteculas
9Caracterizacioacuten interfacial de la primera formulacioacuten de agua en aceite
Para obtener una mejor comprensioacuten del efecto del meacutetodo de formulacioacuten sobre las
propiedades interfaciales de la primera solucioacuten de agua (solucioacuten de lisozima) emulsioacuten en
aceite disentildeamos experimentos de superficie con lisozima y Pluronicreg F68 La diferencia
principal en los dos meacutetodos de formulacioacuten es coacutemo se agrega Pluronicreg F68 en fase acuosa
(W-F68) o en fase orgaacutenica (O-F68) Esta diferencia podriacutea afectar la composicioacuten de la superficie
de las NP y como resultado sus propiedades coloidales
La tensioacuten superficial y la elasticidad en el interfaz aire-agua fueron las propiedades analizadas
(Tabla 3) En esta interfaz las proteiacutenas cambian su conformacioacuten y exponen su parte hidrofoacutebica
al aire dependiendo de su estabilidad termodinaacutemica flexibilidad anfipaticidad tamantildeo
70
molecular y carga En nuestro caso la lisozima es una proteiacutena globular que se adsorbe en la
interfase aire-agua y forma una monocapa riacutegida debido a su estructura interna y la presencia y
cantidad de puentes disulfuro (Pezennec et al 2008) Nuestras mediciones se realizaron a pH 12
por lo tanto la lisozima estaacute cargada negativamente La Tabla 3 muestra la tensioacuten interfacial de
la monocapa de lisozima en la interfaz aire-agua despueacutes de 50 minutos de adsorcioacuten (457 plusmn 04
(mN m)) y su elasticidad (83 plusmn 4 (mN m)) La reduccioacuten de la tensioacuten interfacial en comparacioacuten
con la de la interfaz aire-agua (72 mN m) indica las caracteriacutesticas de tensioactivo de la lisozima
El alto valor de la elasticidad se debioacute a la carga y a las altas interacciones moleculares en la
monocapa de lisozima Cuando la monocapa se forma con Pluronicreg F68 la tensioacuten superficial
es ligeramente menor que con la lisozima cuando se agrega Pluronicreg en AP pero similar
(teniendo en cuenta el error) cuando se agrega en OP
Pluronicreg F68 es una moleacutecula desmontable hiacutebrida que se introduce en el interfaz aire-agua
cuando se disuelve en fase acuosa y tambieacuten cuando se deposita en la superficie de la partiacutecula
Se encuentran pequentildeas diferencias al comparar la tensioacuten superficial de la monocapa Pluronicreg
de los dos meacutetodos Los diferentes valores de tensioacuten interfacial alcanzados en ambos casos se
deben a los diferentes meacutetodos para agregar Pluronicreg F68 a la monocapa de lisozima formada
Pluronicreg F68 presenta una elasticidad menor que la lisozima como se esperaba ya que se sabe
que Pluronicreg F68 forma una monocapa flexible en la interfaz aire-agua (Torcello-Goacutemez et al
2011)
71
Primer Paso
Tensioacuten
Interfacial
(mNm)
Elasticidada
(mNm)
Segundo
Paso
Tensioacuten
Interfacial
(mNm)
Elasticidadb
(mNm)
Lisozima 457plusmn04 83plusmn4 - - -
Lisozima 457plusmn04 83plusmn4 - - -
Pluronicreg
F68 (AP) 421plusmn03 15plusmn3
Pluronicreg
F68 (AP) 379plusmn06 142plusmn05
Pluronicreg
F68 (OP) 475plusmn21 94plusmn05
Pluronicreg
F68 (OP) 38plusmn2 43plusmn4
Tabla 3 Tensioacuten interfacial y elasticidad dilatacional (a 1 Hz) de la interfase aire-agua (a)
despueacutes de adsorber lisozima o Pluronic F68 en la fase acuosa (AP) o Pluronicreg F68 en fase
orgaacutenica (OP) en el primer paso (b) cuando Pluronic F68 se agrega en AP u OP despueacutes de la
adsorcioacuten de la monocapa de lisozima (media plusmn sd n = 3)
Se disentildearon dos ensayos para imitar los meacutetodos de formulacioacuten de las partiacuteculas En el primer
ensayo (meacutetodo W-F68) se formoacute una monocapa de lisozima luego la mayor parte de la
partiacutecula se intercambioacute con la solucioacuten acuosa de Pluronicreg F68 y despueacutes de la adsorcioacuten se
midieron la tensioacuten interfacial y la elasticidad del interfaz (379 plusmn 06 mN my 142 plusmn 05 mN
m respectivamente) Este bajo valor de elasticidad fue muy similar al de la monocapa de
Pluronicreg F68 lo que indica que Pluronicreg F68 se encuentra en el interfaz adecuado y elimina
la lisozima previamente adsorbida En el segundo ensayo (meacutetodo O-F68) despueacutes de que se
formara la monocapa de lisozima Pluronicreg F68 disuelto en cloroformo se depositoacute sobre la
superficie de la partiacutecula El cloroformo se evapora raacutepidamente y se mide la tensioacuten interfacial y
la elasticidad del interfaz (38 plusmn 2 mN my 43 plusmn 4 mN m respectivamente) La elasticidad fue la
mitad de la de la monocapa de lisozima pura tal vez debido a la coexistencia de las moleacuteculas de
72
lisozima y Pluronicreg F68 en el interfaz La tensioacuten superficial del interfaz final no depende del
meacutetodo de adicioacuten de Pluronicreg pero es menor que la de la lisozima pura o que el Pluronicreg
puro
Dentro de este contexto se ha informado ampliamente que la adsorcioacuten de PEO y poloxaacutemeros
en el interfaz reduce la unioacuten a proteiacutenas (Manuel J Santander-Ortega Lozano-Loacutepez et al
2010) (Torcello-Goacutemez et al 2011) En el meacutetodo O-F68 la lisozima se expone al DCM despueacutes
de la formacioacuten de la primera emulsioacuten de agua en aceite incluso si se agrega Pluronicreg ya que
ambos coexisten en el interfaz En el meacutetodo W-F68 la proteiacutena estaraacute en contacto con el acetato
de etilo en este paso ya que el Pluronicreg estaacute ausente Sin embargo este disolvente tiene efectos
bioloacutegicos maacutes deacutebiles sobre la lisozima Pluronicreg podriacutea alcanzar el interfaz cuando se agrega
a la fase acuosa en el siguiente paso y desplazar la proteiacutena del interfaz que podriacutea difundirse
hacia afuera a la fase acuosa
10Morfologiacutea de la partiacutecula
La entrega la biodistribucioacuten y el mecanismo de accioacuten de un faacutermaco o biomoleacutecula
transportada dependen en gran medida del tamantildeo de la partiacutecula la concentracioacuten y el tiempo
(Penaloza et al 2017) En general la escala micromeacutetrica estaacute disentildeada para un suministro local
que permite la formacioacuten de reservorios de la moleacutecula transportada y minimiza la accioacuten del
sistema fagociacutetico (Schwendeman et al 2014) Sin embargo los sistemas nanomeacutetricos son maacutes
versaacutetiles porque permiten una distribucioacuten sisteacutemica son maacutes estables reactivos y permiten la
accioacuten extra e intracelular Este uacuteltimo mecanismo es esencial cuando la moleacutecula o el faacutermaco
debe actuar en el citoplasma (Wang et al 2012) o en cualquier otra estructura intracelular como
la mitocondria el aparato de Golgi el retiacuteculo endoplaacutesmico o el nuacutecleo (Penaloza et al 2017)
(Vasir and Labhasetwar 2007) (Yameen et al 2014) Tambieacuten se han investigado otros
paraacutemetros para alterar el destino intracelular de las partiacuteculas principalmente alterando la
decoracioacuten de su superficie (Sneh-Edri Likhtenshtein and Stepensky 2011) por ejemplo con
sentildeales de localizacioacuten nuclear (NLS) que utilizan el nuacutecleo como el objetivo de la partiacutecula (Vasir
73
and Labhasetwar 2007) Sin embargo estas estrategias auacuten se encuentran en fase de desarrollo
inicial (Penaloza et al 2017) (Yameen et al 2014)
Se buscoacute un tamantildeo de partiacutecula en la escala submicromeacutetrica (entre 2 y 500 nm) ya que es
necesario para la internalizacioacuten celular y una distribucioacuten raacutepida despueacutes de la administracioacuten
parenteral para alcanzar diferentes tejidos a traveacutes de diferentes barreras bioloacutegicas Las partiacuteculas
de menos de 200 nm minimizan su ingesta por macroacutefagos El tipo de disolvente orgaacutenico la
concentracioacuten del poliacutemero la adicioacuten de surfactante y la energiacutea de emulsioacuten controlan el tamantildeo
del sistema
El meacutetodo O-F68 da lugar a una distribucioacuten monomodal del tamantildeo de partiacuteculas con
diaacutemetros alrededor de 100 nm La adicioacuten de Pluronicreg F68 en la fase orgaacutenica refuerza la
estabilidad coloidal de la primera emulsioacuten y reduce el tamantildeo de partiacutecula en comparacioacuten con
las NP de PLGA en las que la estabilidad es puramente electrostaacutetica debido a los grupos
carboxiacutelicos del PLGA En el meacutetodo W-F68 se tienen en cuenta el esfuerzo cortante y el
volumen de la fase acuosa para producir un sistema con partiacuteculas de entre 100 y 500 nm
Las NP de O-F68-Lys tienen una forma esfeacuterica con una distribucioacuten de tamantildeo monomodal
(diaacutemetros alrededor de 100 nm) y una estructura nuacutecleo-capa (Fig 7a) Las partiacuteculas vaciacuteas
producidas con el meacutetodo O-F68 se muestran en las Figs 7 (sin F68) y Fig 8 (con F68) Tambieacuten
son esfeacutericas y con una estructura nuacutecleo-caparazoacuten pero un poco maacutes grande
W-F68-Lys NP tambieacuten presenta una forma esfeacuterica pero una distribucioacuten de tamantildeo
multimodal con diaacutemetros entre 140 y 450 nm la poblacioacuten maacutes grande estaacute alrededor de 260 nm
(Fig 7b) Tambieacuten se observa una estructura nuacutecleo-capa en estas partiacuteculas Las partiacuteculas vaciacuteas
del meacutetodo W-F68 se presentan en la Fig 9 correspondiente a un sistema maacutes polidisperso
74
(a) (b)
(c)
Figura 7 Morfologiacutea de las nanopartiacuteculas Micrografiacuteas de SEM (a b) y STEM (c) de
nanopartiacuteculas vaciacuteas utilizando el meacutetodo O-F68 sin F68
75
(a) (b)
(c) (d)
Figura 8 Micrografias de SEM (a b) y STEM (c d) de partiacuteculas vaciacuteas usando el meacutetodo O-
F68 con F68
76
(a) (b)
Figura 9 Micrografiacuteas de SEM de partiacuteculas vacias usando el meacutetodo W-F68
11Tamantildeo de las nanopartiacuteculas movilidad electrocineacutetica y estabilidad coloidal
La distribucioacuten del diaacutemetro hidrodinaacutemico de las partiacuteculas se determinoacute en primer lugar por
DLS La Tabla 4 contiene las principales propiedades coloidales de las partiacuteculas producidas con
los meacutetodos O-F68 y W-F68 vaciacuteas o cargadas con lisozima Los resultados de partiacuteculas vaciacuteas
del meacutetodo O-F68 pero sintetizados sin F68 tambieacuten estaacuten incluidos
Los paraacutemetros de tamantildeo se calcularon a traveacutes de un anaacutelisis acumulativo de los datos que
es aplicable para distribuciones de tamantildeo monomodal estrechas (Hassan Rana and Verma
2015) Las micrografiacuteas SEM y STEM indican que se podriacutea suponer tal aproximacioacuten para las
partiacuteculas del meacutetodo O-F68 pero no del W-F68 Por lo tanto las distribuciones de tamantildeo de
intensidad de los diferentes sistemas se muestran en la figura 11a La presencia de Pluronicreg F68
en el meacutetodo O-F68 reduce significativamente el tamantildeo y la polidispersidad de las NP Esto
concuerda con la reduccioacuten de la tensioacuten superficial cuando el F68 estaacute en el interfaz (Tabla 3)
lo que promueve el proceso de emulsioacuten Si las NP tambieacuten estaacuten cargadas con lisozima el tamantildeo
es auacuten menor pero la polidispersidad aumenta ligeramente en comparacioacuten con las partiacuteculas
vaciacuteas Las propiedades surfactantes de la lisozima se han demostrado con los resultados de la
tensioacuten superficial (Tabla 3)
La Fig 11a indica la presencia de partiacuteculas superiores a 500 nm con el W-F68 que no se
correlaciona con las micrografiacuteas SEM Por lo tanto se utilizoacute una teacutecnica diferente (NTA) para
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obtener informacioacuten sobre la distribucioacuten del tamantildeo de dichos sistemas (figura 12b) Con NTA
la distribucioacuten de tamantildeos fue consistente con las imaacutegenes SEM Se encontraron distribuciones
de gran tamantildeo correspondientes a los sistemas multimodales con este meacutetodo pero la adicioacuten de
lisozima condujo a una clara reduccioacuten del tamantildeo Esto se debe a que la lisozima tambieacuten actuacutea
como emulsionante en la primera emulsioacuten
La carga electrocineacutetica de las NP se analizoacute midiendo la movilidad electroforeacutetica Para
comparacioacuten todas las muestras se midieron a pH 7 (tampoacuten de fosfato) En la Fig 12 se
presentan las distribuciones de movilidad electroforeacutetica mientras que los promedios
correspondientes se muestran en la Tabla 4
Las NP de PLGA normalmente estaacuten cargadas de forma negativa debido a los grupos
carboxiacutelicos del poliacutemero El uso de Pluronicreg F68 en el meacutetodo O-F68 reduce claramente la
movilidad electroforeacutetica de las NP lo que indica que algo de Pluronicreg estaacute ubicado en la
superficie de las NP Esta reduccioacuten se esperaba despueacutes de la incorporacioacuten de este tensioactivo
no ioacutenico al interfaz ya que la presencia de cadenas de oacutexido de polietileno causariacutea un
desplazamiento hacia afuera del plano de corte donde se define el potencial y esto disminuiriacutea
posteriormente la movilidad electroforeacutetica Los resultados previos para partiacuteculas de PLGA han
mostrado una reduccioacuten significativa directamente relacionada con el recubrimiento de
poloxaacutemero (Santander-Ortega et al 2006) Si comparamos los dos sistemas la superficie menos
negativa para las NP OF68 se relacionariacutea con una menor densidad del poliacutemero PLGA de
superficie llevando la carga eleacutectrica negativa a el interfaz Este resultado estariacutea en liacutenea con la
mayor cantidad de PLGA en la formulacioacuten del nanosistema WF68
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Figura 10 Micrografiacuteas SEM y STEM de partiacuteculas cargadas de lisozima usando el meacutetodo
O-F68 (a) o W-F68 (b)
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Media Z (nm) PDI Media-μ (micromcmVs)
Meacutetodo
O-F68
Vaciacuteo sin F68 266 plusmn 7 0293 -506 plusmn 015
Vaciacuteo 1627 plusmn 21 0081 -429 plusmn 018
Cargada de
Lisozima 1210 plusmn12 0244 -334 plusmn 007
Meacutetodo
W-F68
Vaciacuteo 273 plusmn 3 0193 -531 plusmn 011
Cargada de
Lisozima 293 plusmn 4 0169 -4212 plusmn 0013
Tabla 4 Propiedades coloidales de PLGA NP de diferentes meacutetodos de formulacioacuten Se
midieron en tampoacuten de fosfato (pH 7) El diaacutemetro hidrodinaacutemico promedio (media Z o media
acumulada) y el iacutendice de polidispersidad (PDI) se determinan a partir de DLS (Media plusmn sd n
= 3)
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(a)
(b)
Figura 11 Distribucioacuten del diaacutemetro hidrodinaacutemico (a) mediante DLS a pH 7 (tampoacuten de
fosfato) de partiacuteculas de PLGA vaciacuteas y cargadas con lisozima de los meacutetodos O-F68 y W-F68
y (b) mediante NTA a pH 7 (tampoacuten de fosfato) de partiacuteculas de PLGA vaciacuteas y cargadas con
lisozima del meacutetodo W-F68
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(a)
(b)
Figura 12 Distribucioacuten de movilidad electroforeacutetica a pH 7 (tampoacuten de fosfato) de partiacuteculas
de PLGA cargadas con lisozima y vaciacuteas a partir de los meacutetodos (a) O-F68 y (b) W-F68
82
Cuando la lisozima tambieacuten se utiliza en la siacutentesis la superficie es auacuten menos negativa lo que
podriacutea explicarse por la presencia de alguna proteiacutena (cuya carga neta es positiva) cerca o en el
interfaz Este uacuteltimo efecto tambieacuten se encuentra con el meacutetodo W-F68 La atractiva interaccioacuten
electrostaacutetica entre los residuos de aacutecido terminales negativos de PLGA y las moleacuteculas de
lisozima juega un papel clave en el proceso de encapsulacioacuten de proteiacutenas (Paillard-Giteau et al
2010) o adsorcioacuten (Cai et al 2008) en PLGA NPs que afecta la carga final de proteiacutenas
En relacioacuten con esta situacioacuten una caracteriacutestica importante de la formulacioacuten de
encapsulacioacuten W-F68 es que la fase acuosa tiene un pH de 12 lo que permite una carga neta
negativa de lisozima y por lo tanto evita la atraccioacuten electrostaacutetica de la proteiacutena y el poliacutemero
Esta situacioacuten puede reducir la eficacia de la encapsulacioacuten pero al mismo tiempo favorece el
posterior proceso de difusioacuten de proteiacutenas y en consecuencia la liberacioacuten a corto plazo
Estudios recientes han propuesto el uso de nanopartiacuteculas incrustadas en scaffolds impresos en
3D predisentildeados (Baumann et al 2017) (Lee et al 2017) lo que nos lleva a analizar la
estabilidad de las dos formulaciones en varios medios generalmente empleados durante la
preparacioacuten de otras estructuras Se encontraron distribuciones de tamantildeo similares al original
para las dos formulaciones en diferentes medios (PB PBS y DMEM) y en diferentes momentos
despueacutes de la siacutentesis (0 1 y 5 diacuteas) La carga eleacutectrica de los grupos terminales de aacutecido PLGA
y las moleacuteculas de poloxaacutemero ubicadas en la superficie NP confiere un mecanismo de estabilidad
coloidal combinado electrostaacutetico y esteacuterico como se ha descrito previamente (Manuel J
Santander-Ortega Lozano-Loacutepez et al 2010) (Santander-Ortega et al 2006) Ademaacutes las NPs
en todos los casos mantienen su tamantildeo almacenado a 4 deg C al menos durante 1 mes (datos no
mostrados) Por lo tanto los medios descritos podriacutean usarse potencialmente como medios de
almacenamiento o para preparar otras soluciones o scaffolds antes de colocarlos realmente en el
entorno vivo (in vitro o in vivo)
83
12NMR de las nanopartiacuteculas
En la Fig 12 tanto las NP vaciacuteas como las cargadas de proteiacutenas presentan una movilidad
electroforeacutetica menos negativa que las NP vaciacuteas sin F68 lo que podriacutea explicarse por la presencia
de Pluronicreg F68 en la superficie de la NP Al comparar los espectros 1HNMR de Pluronicreg F68
libre y las NP cargadas con lisozima de los meacutetodos O-F68 y W-F68 podemos verificar la
presencia de F68 en la superficie de las NP (figura 13) por los picos que se muestran entre 325 y
375 ppm y a 1 ppm Estos picos tambieacuten son visibles en los espectros de NP formulados con F68
(O-F68 y W-F68 figuras 14 y 15 respectivamente)
RMN de las nanopartiacuteculas
Figura 13 Espectro 1HMNR de F68 libre
84
Figura 14 Espectro de 1HMNR de partiacuteculas cargadas con lisozima del meacutetodo O-F68
Figura 15 Espectro de 1HMNR de partiacuteculas cargadas con lisozima del meacutetodo W-F68
433 Actividad bioloacutegica e interacciones
F68
F68
F68
F68
85
Una liberacioacuten controlada desde un sistema de administracioacuten basado en PLGA es una tarea
difiacutecil ya que depende de muacuteltiples factores el tipo de PLGA el solvente el estreacutes mecaacutenico el
uso de surfactantes etc (Hines and Kaplan 2013) La difusioacuten de la proteiacutena y la erosioacuten del
poliacutemero son los principales mecanismos implicados en la liberacioacuten de proteiacutena en los sistemas
de administracioacuten basados en PLGA Ademaacutes es tiacutepico encontrar una liberacioacuten en forma de una
raacutepida raacutefaga en la etapa inicial seguida de una fase de liberacioacuten lenta en un corto y mediano
plazo En esta fase las moleacuteculas de proteiacutena se difunden a traveacutes de la matriz del poliacutemero hasta
alcanzar una fase final en la que la degradacioacuten del poliacutemero por hidroacutelisis permite una liberacioacuten
maacutes raacutepida (Fredenberg et al 2011)
Por otro lado la liberacioacuten a corto plazo es de especial intereacutes para el transporte de factores de
crecimiento morfogeneacuteticos oacuteseos (BMP) Una explosioacuten inicial controlada seguida de una
liberacioacuten sostenida mejora significativamente la regeneracioacuten in vivo de hueso (Ortega-Oller et
al 2015) y cartiacutelago (Begam et al 2017) incluso en sistemas de liberacioacuten controlada doble
(Kim and Tabata 2015) Por estas razones centramos nuestro anaacutelisis en la liberacioacuten a corto
plazo teniendo en cuenta la reduccioacuten de la degradacioacuten del poliacutemero por hidroacutelisis encontrada
en sistemas similares para estos primeros pasos (Rescignano et al 2016)
86
Figura 16 Liberacioacuten acumulada (siacutembolos rellenos) y bioactividad residual (siacutembolos
abiertos) de O-F68-Lys (cuadrado) y W-F68-Lys (triaacutengulo) incubados durante diferentes
tiempos a 37 deg C en tampoacuten de fosfato salino (pH 74) (media plusmn sd n = 3)
La Fig 16 muestra la liberacioacuten acumulativa de lisozima de las NP de O-F68-Lys a corto plazo
(siete diacuteas) Estos resultados son consistentes con un proceso de dos pasos un estallido inicial y
uno de liberacioacuten lenta El primer paso podriacutea corresponder a la liberacioacuten de las moleacuteculas de
proteiacutena ubicadas cerca de la superficie cuya presencia se dedujo de los resultados de movilidad
electroforeacutetica (Fig 12) La segunda parte del proceso de liberacioacuten fue limitada y lenta debido a
la difusioacuten de proteiacutenas a traveacutes de la matriz de la cubierta polimeacuterica La interaccioacuten electrostaacutetica
especiacutefica entre las moleacuteculas de lisozima positiva y los grupos dxe aacutecido terminal negativo de
PLGA puede reducir la difusioacuten de proteiacutenas (Blanco and Alonso 1998) Cuando se agrega el
poloxaacutemero (F68) la interaccioacuten entre el surfactante y la proteiacutena ayuda al proceso de difusioacuten
lo que lleva a una liberacioacuten maacutes completa y sostenida (Manuel J Santander-Ortega Csaba et
87
al 2010) Tambieacuten ayuda a mantener la actividad bioloacutegica de la proteiacutena (Paillard-Giteau et al
2010) (Morille et al 2013) El poloxaacutemero reduce las interacciones proteiacutena-poliacutemero no
especiacuteficas (es decir interacciones hidroacutefobas) pero no las especiacuteficas (electrostaacutetica) por lo
tanto la difusioacuten a traveacutes de los poros llenos de agua o a traveacutes del poliacutemero sigue siendo limitada
En el estudio actual la fraccioacuten de proteiacutena liberada y el patroacuten de liberacioacuten son similares a los
encontrados en la literatura para la lisozima encapsulada en nano y micropartiacuteculas de mezclas de
PLGA y otros poliacutemeros o surfactantes (Meng et al 2003) (White et al 2013) (Perez De Jesus
and Griebenow 2002)
La curva de liberacioacuten de proteiacutenas de W-F68-Lys NP (Fig 16) revela que la tasa de
administracioacuten inicial es ideacutentica a la del sistema O-F68 lo que podriacutea significar una proporcioacuten
similar de proteiacutena encapsulada cerca o en la superficie para ambos sistemas NP Esto estariacutea de
acuerdo con la disminucioacuten anaacuteloga en la movilidad electroforeacutetica de las NP cargadas con
lisozima de la que informaron previamente (Fig 12) En la segunda parte del proceso la
interaccioacuten especiacutefica entre la proteiacutena y el poliacutemero estaacute nuevamente presente Sin embargo el
proceso de difusioacuten en el sistema W-F68 parece mejorar permitiendo una liberacioacuten continua y
sostenida despueacutes del estallido inicial y alcanzando un valor ligeramente maacutes alto para el tiempo
de liberacioacuten maacuteximo estudiado Este resultado podriacutea estar relacionado con la estructura interna
de la capa de poliacutemero que permite una mejor hidratacioacuten y por lo tanto una mejor difusioacuten de
la proteiacutena hacia el exterior Se ha informado previamente que el uso de disolventes orgaacutenicos
menos polares tales como DCM para formulaciones de partiacuteculas de PLGA aumenta la densidad
de la matriz de poliacutemero en comparacioacuten con disolventes orgaacutenicos maacutes polares tales como EA
Las matrices PLGA resultan maacutes resistentes en el primer caso pero reducen al mismo tiempo su
conectividad y difusioacuten (Bohr et al 2015) Meng y colaboradores (Meng et al 2003) encontraron
que una eliminacioacuten maacutes raacutepida de EA da como resultado una liberacioacuten cineacutetica maacutes lenta de la
proteiacutena debido a una disminucioacuten en la porosidad de las NP En cuanto al papel de Pluronicreg
Rafati y colaboradores (Rafati et al 2012) encontraron una mayor concentracioacuten de proteiacutena
88
encapsulada en los poros superficiales en micropartiacuteculas sintetizadas en presencia de surfactante
en la segunda fase acuosa de la emulsioacuten Dado que se introdujo un paso intermedio en nuestra
formulacioacuten W-F68 en la segunda fase acuosa de la emulsioacuten la eliminacioacuten de la EA por difusioacuten
se controloacute fuertemente de modo que se esperaba que la porosidad de estas NP aumentara Esta
porosidad mejora la difusioacuten de proteiacutenas lo que permite un patroacuten de liberacioacuten maacutes estable de
acuerdo con el resultado experimental encontrado para este sistema A pesar del efecto
desfavorable de la interaccioacuten proteiacutena-poliacutemero electrostaacutetico especiacutefico en la liberacioacuten la
cantidad de proteiacutena liberada en nuestras NP es sustancial lo que significa que hay otras
interacciones inespeciacuteficas que pueden ser moduladas por la presencia de surfactante permitiendo
un lanzamiento sostenido La cantidad de lisozima liberada es similar a la encontrada con la
lisozima fiacutesicamente adsorbida en la superficie de las nanopartiacuteculas de PLGA a pesar de la
atraccioacuten electrostaacutetica (Cai et al 2008) Ademaacutes de otras interacciones inespeciacuteficas la
concentracioacuten de electrolitos en el medio de liberacioacuten podriacutea modular esta atraccioacuten
electrostaacutetica entre la proteiacutena y el poliacutemero disminuyeacutendola y facilitando el proceso de
liberacioacuten (Manuel J Santander-Ortega Lozano-Loacutepez et al 2010)
Otro paraacutemetro notable es la actividad bioloacutegica de la liberacioacuten in vitro de lisozima que se
muestra en la Fig 16 Mientras que en el sistema O-F68 la bioactividad se reduce parcialmente
hasta en un 40 la proteiacutena suministrada por el sistema W-F68 mantiene la actividad por encima
del 90 con respecto a la de la lisozima suministrada comercialmente y se resuspende en el mismo
tampoacuten de liberacioacuten Como se discutioacute anteriormente tanto el solvente orgaacutenico como la
interaccioacuten hidrofoacutebica entre la proteiacutena y el poliacutemero a menudo causan la desnaturalizacioacuten de
las proteiacutenas encapsuladas (Paillard-Giteau et al 2010) (Gaudana et al 2013) Perez y
colaboradores (Perez De Jesus and Griebenow 2002) describen una peacuterdida parcial de actividad
cuando se usa DCM y una solucioacuten acuosa de PVA en la segunda etapa de emulsioacuten sin ninguacuten
excipiente adicional El uso de poloxaacutemeros en la formulacioacuten reduce tales interacciones mejora
la estabilidad de la proteiacutena y mantiene una capa acuosa que retiene las moleacuteculas de agua
89
necesarias para la funcioacuten bioloacutegica de la proteiacutena al mismo tiempo que ayuda a su difusioacuten Esta
situacioacuten junto con el uso de un solvente orgaacutenico deacutebil como EA ayuda a preservar la actividad
bioloacutegica de la lisozima como se encuentra para el sistema W-F68-Lys
La Fig 17 presenta diferentes imaacutegenes de microscopiacutea confocal relacionadas con el proceso
de liberacioacuten de NPs W-F68 cargadas de lisozima Una disminucioacuten en la intensidad de
fluorescencia fue apreciable a lo largo del experimento in vitro Ademaacutes la agregacioacuten del sistema
es visible a medida que avanza el proceso de incubacioacuten El anaacutelisis de estas imaacutegenes es
consistente con los resultados informados previamente para este sistema NP
90
Figura 17 Actividad bioloacutegica e interacciones Imaacutegenes octogonales obtenidas por
microscopiacutea confocal de Lisozima-FITC encapsulado en NPs W-F68 tras su incubacioacuten a
diferentes tiempos en tampon fosfato salino (pH 74) a 37ordmC Las NP se centrifugaron previamente
para eliminar la proteiacutena marcada liberada (a) Experimentos previos al lanzamiento (b)
despueacutes de 24h (c) despueacutes de 72h (d) despueacutes de 168h
13Captacioacuten celular
La captacioacuten celular de NP de PLGA es un proceso conocido que se ve afectado principalmente
por las propiedades de superficie la funcionalizacioacuten (Loureiro et al 2016) y la agregacioacuten de
partiacuteculas (Xiong et al 2011) La internalizacioacuten y el procesamiento intracelular posterior de las
partiacuteculas se ha descrito como un proceso activo por lo tanto depende de la energiacutea y puede
verse afectado por otros factores que alteran la absorcioacuten de energiacutea por parte de las ceacutelulas como
la temperatura (Penaloza et al 2017) Las partiacuteculas pueden internalizarse mediante varios
meacutetodos de endocitosis que dependen principalmente del tamantildeo de la partiacutecula partiacuteculas
(a) (b)
(c) (d)
91
dependientes de caveolina (diaacutemetro asymp 60 nm) independientes de clatrina (diaacutemetro asymp 90 nm) y
dependientes de clatrina (diaacutemetro asymp 120 nm) (Vasir and Labhasetwar 2007) (Yameen et al
2014) Una vez internalizados alrededor del 65 se exportan al espacio extracelular antes de
liberar cualquiera de sus contenidos mientras que el resto libera lentamente la moleacutecula
encapsulada en el espacio intracelular (Panyam and Labhasetwar 2003)
Figura 18 Proyeccioacuten z de 5 imaacutegenes de hMSCs visualizadas 30 minutos despueacutes de la
incubacioacuten con W-F68-LysFITCNPsorO-F68-LysFITCNPs Las hMSC se marcaron previamente
con Guiacutea celular roja Barra de escala 20 m
El proceso de liberacioacuten intracelular se ve afectado por la formulacioacuten de las partiacuteculas
(Penaloza et al 2017) Hemos demostrado que los sistemas propuestos siguen un patroacuten similar
a otros publicados anteriormente En un periodo de tiempo tan corto como 30 minutos despueacutes
de la incubacioacuten las NPs de W-F68-LysFITC fueron absorbidas por las ceacutelulas (Fig 18) Algunas
partiacuteculas W-F68 todaviacutea estaban en el medio por lo que la actividad dual podriacutea ocurrir Por el
92
contrario las NP de O-F68-LysFITC se vieron afectadas por la agregacioacuten y por lo tanto no
alcanzaron adecuadamente el espacio intracelular (Fig 18 para las imaacutegenes del eje Z veacutease la
Fig 19)
Superposicioacuten Canal Verde Canal Rojo P
royec
cioacuten z
93
Superposicioacuten Canal Verde Canal Rojo
Pro
yec
cioacuten z
94
Figura 19 Proyeccioacuten z de 5 imaacutegenes e imaacutegenes z independientes de hMSC visualizadas 30
minutos despueacutes de la incubacioacuten sin partiacuteculas y con NP W-F68-LysFITC o NP O-F68-LysFITC
Las hMSC se marcaron previamente con un rastreador celular rojo Barra de escala 20 microm
Pro
yec
cioacuten z
Superposicioacuten Canal Verde Canal Rojo
95
Esto contradice los anaacutelisis previos de la estabilidad coloidal en PB PBS y DMEM Este
hallazgo puede explicarse por el hecho de que aunque el medio de cultivo fue DMEM este uacuteltimo
medio se complementoacute con suero fetal bovino y las ceacutelulas liberan muchos factores al medio
extracelular que pueden afectar a este tipo de partiacuteculas Ninguno de los sistemas demostroacute ser
toacutexico para las ceacutelulas (Fig 20) No hay estudios disponibles que hayan informado alguacuten efecto
de la lisozima sobre las hMSC
Figura 20 Pruebas de viabilidad a las 6 horas de antildeadir diferentes dosis de partiacuteculas A
continuacioacuten se separaron las ceacutelulas se tintildeeron con azul tripaacuten y se contaron las ceacutelulas vivas
o muertas El graacutefico representa ceacutelulas vivas normalizadas y la desviacioacuten estaacutendar de tres
experimentos No se encuentran diferencias
0
02
04
06
08
1
12
14
16
Control W-F68
10microlml
W-F68
5microlml
O-F68
10microlml
O-F68 5microlml
Viability after 6 hours
Viabilidad 6 horas despueacutes
96
5FORMULACIOacuteN CARACTERIZACIOacuteN COLOIDAL Y EFECTO
BIOLOacuteGICO IN VITRO DE NANOPARTIacuteCULAS DE PLGA
CARGADAS CON BMP-2 PARA LA REGENERACIOacuteN OacuteSEA
51 Antecedentes
En el contexto de la nanomedicina la regeneracioacuten de tejidos usando micro y nano estructuras
coloidales que tienen un tamantildeo y actividad superficial uacutenicos ha recibido una atencioacuten creciente
en los uacuteltimos antildeos Se han realizado muchos esfuerzos para mejorar la ingenieriacutea de estos nano-
sistemas con el fin de alcanzar una entrega inteligente de moleacuteculas bioactivas para optimizar
sus ventajas terapeacuteuticas y minimizar los efectos secundarios nocivos (van Rijt and Habibovic
2017) Con este objetivo hay descrito un amplio espectro de nanoportadores biocompatibles que
muestran propiedades adecuadas para diferentes aplicaciones bioloacutegicas y terapeacuteuticas (Kumar et
al 2017) Entre estas variadas propuestas los nanosistemas polimeacutericos representan un grupo
importante siendo el PLGA uno de los maacutes utilizados debido a las propiedades comentadas
anteriormente a lo largo de este trabajo entre ellas destaca su biocompatibilidad
biodegradabilidad y baja citotoxicidad asi como su capacidad para administrar una amplia
variedad de moleacuteculas y faacutermacos activos moleacuteculas sinteacuteticas o naturales con propiedades
hidrofiacutelicas o hidrofoacutebicas y biomoleacuteculas de proteiacutenas a aacutecidos nucleicos (Danhier et al 2012)
(Ding and Zhu 2018) (Arias et al 2015) obteniendo la aprobacioacuten de diferentes agencias
farmaceacuteuticas para uso humano (Mir Ahmed and Rehman 2017) (Jana and Jana 2017)
Dentro de la ingenieriacutea de tejidos en el campo de la odontologiacutea las teacutecnicas de regeneracioacuten
oacutesea empiezan a despertar un gran intereacutes por parte de los diferentes profesionales de la salud
contribuyendo con ello a un mayor desarrollo de estas liacuteneas de investigacioacuten y generando una
mayor tendencia a diferentes viacuteas de crecimiento relacionadas con el subsanamiento de los
obstaacuteculos que pueden originarse durante el proceso de encapsulacioacuten de moleacuteculas hidrofilicas
o en el de funcionalizacioacuten especiacutefica de la superficie a fin de mejorar la versatilidad de las
diferentes moleculas supuestos eacutestos en los que el PLGA es el poliacutemero de referencia para la
97
comunidad cientiacutefica en aras de crear NP para favorecer la cicatrizacioacuten oacutesea (Bapat et al 2019)
La literatura describe el suministro de moleacuteculas bioactivas normalmente factores de crecimiento
utilizando micropartiacuteculas polimeacutericas (MP) y NP con PLGA como componente principal
(Ortega-Oller et al 2015) Entre los factores de crecimiento morfogeneacutetico oacuteseo la BMP-2
(proteiacutena morfogeneacutetica oacutesea 2) ha sido la maacutes citada con muchos ejemplos en los que la
encapsulacioacuten o la adsorcioacuten en la superficie permite una eficiencia de atrapamiento adecuada y
diversos patrones de liberacioacuten (Ji et al 2010) (Kirby et al 2011) (Qutachi Shakesheff and
Buttery 2013) (Wang et al 2015) (Zhang et al 2016) Para las proteiacutenas con una vida media
muy corta como las BMP los nanosistemas PLGA biodegradables proporcionan proteccioacuten y
una dosis oacuteptima para una estimulacioacuten adecuada de la diferenciacioacuten celular (Begam et al 2017)
(Balmayor et al 2009)
Por lo tanto dentro de este escenario en el presente trabajo buscamos optimizar un sistema
de nanopartiacuteculas para llevar a cabo y controlar la liberacioacuten de BMP-2 utilizando como punto de
partida el procedimiento de siacutentesis de un sistema de NP cargado de lisozima previamente
descrito para la encapsulacioacuten de ese modelo de proteiacutena (Ortega-Oller et al 2017) Ademaacutes
para encapsular BMP-2 preparamos un segundo sistema en el que esta proteiacutena se co-adsorbioacute
con albuacutemina de suero bovino en la superficie de NP vaciacuteas Hemos estudiado el tamantildeo y la
morfologiacutea la eficiencia de la encapsulacioacuten de proteiacutenas las caracteriacutesticas de la superficie y la
estabilidad coloidal y temporal para completar la caracterizacioacuten fisicoquiacutemica de ambos sistemas
NP
El perfil de liberacioacuten de BMP-2 indica el potencial de un nanoportador PLGA para la
regeneracioacuten oacutesea y depende en gran medida de la degradacioacuten del poliacutemero por hidroacutelisis (Xu et
al 2017) Sin embargo a corto plazo durante el cual la liberacioacuten no depende de esta degradacioacuten
quiacutemica es necesario un control adecuado de la liberacioacuten para modular otros procesos fiacutesicos
Por lo tanto enfocamos nuestros experimentos de liberacioacuten a corto plazo utilizando diferentes
teacutecnicas para comparar las dos muestras de NP y establecer los perfiles de liberacioacuten de BMP-2
98
correspondientes Finalmente la actividad bioloacutegica (migracioacuten celular proliferacioacuten y
diferenciacioacuten osteogeacutenica) se proboacute in vitro utilizando ceacutelulas estromales mesenquimales (MSC)
derivadas del hueso alveolar (Padial-Molina et al 2019)
52 Materiales y Meacutetodos
521Siacutentesis de nanoparticulas
14Formulacioacuten
El aacutecido poli (lactico-co-glicolico) (PLGA 5050) ([C2 H2 O2]x [C3 H4 O2]y) x = 50 y = 50
(Resomerreg 503H) 32 - 44 kDa se usoacute como poliacutemero El surfactante polimeacuterico Pluronicreg F68
(Poloxaacutemero 188) (Sigma-Aldrich) se usoacute como el emulsionante Su estructura se basa en un
copoliacutemero tri-bloque de poli (oacutexido de etileno) PEO y poli (oacutexido de propileno) PPO (PEO)a ndash
(PPO)b ndash (PEO)a con a = 75 y b = 30 La lisozima de huevo de gallina (Sigma-L7651) se usoacute
como proteiacutena hidroacutefila La proteiacutena morfogeneacutetica oacutesea recombinante humana rhBMP-2
(Sigma-H4791) se utilizoacute como biomoleacutecula terapeacuteutica El agua se purificoacute en un sistema Milli-
Q Academic Millipore Se usoacute un meacutetodo de siacutentesis de doble emulsioacuten siguiendo un
procedimiento previamente descrito con ligeras modificaciones (Ortega-Oller et al 2017) En
este meacutetodo se disolvieron 100 mg de PLGA y 3 mg de aacutecido desoxicoacutelico (DC) en un tubo que
conteniacutea 1 ml de acetato de etilo (EA) y se sometieron a voacutertice En total se agregaron 40 μL de
una solucioacuten tamponada a pH 128 con o sin rhBMP-2 (200 μg mL) y se sonicoacute de inmediato
(Branson Ultrasonics 450 Analifier Sonifier) durante 1 min (Dial del ciclo de trabajo 20 Dial
de control de salida 4) con el tubo rodeado de hielo Esta emulsioacuten primaria de WO se vertioacute en
un tubo de plaacutestico que conteniacutea 2 ml de una solucioacuten tamponada (pH 12) de F68 a 1 mg ml y
se agitoacute en voacutertex durante 30 s Luego el tubo rodeado de hielo se sonicoacute a la amplitud maacutexima
de la micro punta durante 1 minuto (control de salida 7) Esta segunda emulsioacuten WOW se vertioacute
en un vaso que conteniacutea 10 ml de la solucioacuten F68 tamponada y se mantuvo bajo agitacioacuten
magneacutetica durante 2 minutos El disolvente orgaacutenico se extrajo raacutepidamente por evaporacioacuten al
99
vaciacuteo hasta un volumen final de 8 ml Los sistemas de NP encapsulados vaciacuteos y BMP-2
resultantes se denominaron NP y NP-BMP2 respectivamente En la Figura 21 se muestra un
esquema detallado del procedimiento de siacutentesis con un rendimiento basado en el componente
PLGA siempre superior al 85
15Limpieza y almacenamiento
Despueacutes de la evaporacioacuten del solvente orgaacutenico la muestra se centrifugoacute durante 10 minutos
a 20 deg C a 12000 rpm El sobrenadante se filtroacute usando nanofiltros Millipore 01 μm para medir
la proteiacutena libre no encapsulada El sedimento se resuspendioacute en tampoacuten fosfato (NaH2PO4 115
mM) PB hasta un volumen final de 4 ml y se mantuvo refrigerado a 4ordmC En estas condiciones
los sistemas mantuvieron la estabilidad coloidal al menos durante un mes
16Carga de proteiacutenas y eficiencia de encapsulacioacuten
La carga inicial de proteiacutenas se optimizoacute para la formulacioacuten de nanopartiacuteculas preservando
la estabilidad coloidal final despueacutes de la etapa de evaporacioacuten y teniendo en cuenta las cantidades
mostradas en la literatura para este factor de crecimiento cuando se encapsula dentro de NPs de
PLGA (drsquoAngelo et al 2010) (Chang et al 2017) Por lo tanto elegimos 2 μg como la masa total
inicial de rhBMP-2 lo que significa una relacioacuten de 2 10 5 p p (rhBMP-2 PLGA) La
cantidad de rhBMP-2 encapsulado se calculoacute midiendo la diferencia entre la cantidad agregada
inicial y la proteiacutena libre no encapsulada presente en el sobrenadante despueacutes de la etapa de
limpieza que se proboacute mediante un ensayo inmuno-absorbente ligado a enzimas especiacuteficas
siguiendo las instrucciones del fabricante (ELISA kit RAB0028 de Sigma-Aldrich St Louis
MO EE UU) Luego la eficiencia de encapsulacioacuten de proteiacutenas (EE) se calculoacute de la siguiente
manera
EE = 119872119868minus119872119865
119872119868119909 100
donde MI es la masa total inicial de rhBMP-2 y MF es la masa total de rhBMP-2 en el
sobrenadante acuoso
100
17Adsorcioacuten Fiacutesica de Proteiacutenas
La albuacutemina de suero bovino (BSA) y la rhBMP-2 se acoplaron en la superficie de
nanopartiacuteculas vaciacuteas mediante un meacutetodo de adsorcioacuten fiacutesica El volumen apropiado de una
solucioacuten de proteiacutena acuosa que conteniacutea 05 mg de BSA y 2 μg de rhBMP-2 se mezcloacute con 5 ml
de tampoacuten de acetato (pH 5) que conteniacutea NP vaciacuteas con 125 mg de PLGA Esto proporcionoacute
una cantidad inicial de proteiacutenas correspondiente al 004 p p (proteiacutena PLGA) mientras que
la relacioacuten de masa entre proteiacutenas fue de 04 p p (rhBMP-2 BSA) Esta solucioacuten se incuboacute a
temperatura ambiente durante 2 h con agitacioacuten mecaacutenica Las nanopartiacuteculas se separaron de la
solucioacuten de tampoacuten por centrifugacioacuten y despueacutes de que se filtraran los sobrenadantes
(nanofiltros Millipore 01 μm) se analizaron cualitativamente por electroforesis en gel mientras
que la cuantificacioacuten de proteiacutenas se realizoacute mediante un ensayo de proteiacutena de aacutecido
bicinconiacutenico (BCA) (Sigma-Aldrich St Louis MO EE UU) Para BSA y el ELISA especiacutefico
para rhBMP-2 El sedimento de nanopartiacuteculas se resuspendioacute en tampoacuten fosfato (pH 74) y se
almacenoacute a 4ordmC Este sistema se denominoacute NP-BSA-BMP2
18Separacioacuten de proteiacutenas por electroforesis en gel SDS-PAGE
Las NP cargadas de proteiacutena y los diferentes sobrenadantes se trataron a 90 deg C durante 10
minutos en el siguiente tampoacuten Tris-HCl 625 mM (pH 68 a 25 deg C) dodecil sulfato de sodio al
2 (p v) (SDS) 10 de glicerol 001 (p v) de azul de bromofenol ditiotreitol (DTT) 40
mM Las muestras se separaron luego por tamantildeo en gel de poliacrilamida poroso al 12
(electroforesis en gel de poliacrilamida SDS 1D) bajo el efecto de un campo eleacutectrico La
electroforesis se realizoacute a voltaje constante (130 V 45 min) y los geles se tintildeeron usando una
solucioacuten de azul de Coomassie (01 de azul brillante de Coomassie R-250 50 de metanol y
10 de aacutecido aceacutetico glacial) y se destintildeeron con la misma solucioacuten que carece del tinte
101
522 Caracterizacioacuten de nanopartiacuteculas morfologiacutea tamantildeo concentracioacuten y movilidad
electrocineacutetica
Se obtuvieron imaacutegenes de NP mediante microscopiacutea electroacutenica de barrido (SEM) con un
microscopio electroacutenico de barrido de emisioacuten de campo SUPRA 40VP Zeiss del Centro de
Instrumentacioacuten Cientiacutefica de la Universidad de Granada (CIC UGR)
La distribucioacuten del tamantildeo hidrodinaacutemico de las NP se evaluoacute mediante anaacutelisis de
seguimiento de nanopartiacuteculas (NTA) con un NanoSight LM10-HS (GB) FT14 (NanoSight
Amesbury Reino Unido) y una caacutemara sCMOS La concentracioacuten de partiacuteculas de acuerdo con
el diaacutemetro (distribucioacuten de tamantildeos) se calculoacute como un promedio de al menos tres
distribuciones de tamantildeos independientes La concentracioacuten total de NP de cada sistema se
determinoacute para controlar el nuacutemero de partiacuteculas utilizadas en los experimentos celulares Las
condiciones de medicioacuten para todas las muestras fueron 25 deg C una viscosidad de 089 cP un
tiempo de medicioacuten de 60 s y una ganancia de caacutemara de 250 El obturador de la caacutemara fue de
11 y 15 ms para las NP vaciacuteas y cargadas con BMP respectivamente El umbral de deteccioacuten se
fijoacute en 5
La movilidad electroforeacutetica de las NP se determinoacute utilizando un dispositivo Zetasizerreg
NanoZeta ZS (Malvern Instrument Ltd Malvern Reino Unido) que funciona a 25 deg C con un
laacuteser He-Ne de 633 nm y un aacutengulo de dispersioacuten 173 Cada punto de datos se tomoacute como un
promedio sobre tres mediciones de muestra independientes Para cada muestra la distribucioacuten de
movilidad electroforeacutetica y la movilidad electroforeacutetica promedio (μ-promedio) se determinaron
mediante la teacutecnica de electroforesis Doppler laacuteser
523 Estabilidad coloidal y temporal en medios bioloacutegicos
El diaacutemetro hidrodinaacutemico promedio y el iacutendice de polidispersidad (PDI) por dispersioacuten
dinaacutemica de la luz (DLS) de cada sistema de NP se midieron en diferentes medios (tampoacuten de
fosfato (PB) tampoacuten de fosfato salino (PBS) y medio de cultivo celular Medio de Eagle
modificado de Dulbecco DMEM (Sigma)) Ademaacutes los datos sobre la estabilidad temporal se
102
obtuvieron repitiendo estos anaacutelisis en diferentes momentos despueacutes de la siacutentesis (0 1 y 5 diacuteas)
y despueacutes de 1 mes en condiciones de almacenamiento
Los experimentos de liberacioacuten in vitro se realizaron de la siguiente manera 1 ml de cada
muestra para cada tiempo de incubacioacuten se suspendioacute en PBS a 37 deg C Despueacutes del tiempo
correspondiente (24 48 96 168 h) las NP se separaron del sobrenadante de las proteiacutenas
liberadas por centrifugacioacuten durante 10 min a 14000 rpm (10 C) El sedimento de NP se suspendioacute
en 1 ml de NaOH 005 M y se agitoacute durante 2 h para una degradacioacuten completa del poliacutemero La
solucioacuten de proteiacutena alcalina se analizoacute mediante BCA y ELISA para cuantificar la cantidad
ineacutedita La proteiacutena liberada se calculoacute teniendo en cuenta la cantidad encapsulada total Todos
los experimentos se realizaron por triplicado
524 Interacciones celulares
Para todos los estudios bioloacutegicos in vitro se utilizoacute una poblacioacuten celular cultivada del hueso
alveolar maxilar Esta poblacioacuten se caracterizoacute previamente y se confirmoacute que presentaba todas
las caracteriacutesticas de una poblacioacuten de ceacutelulas del estroma mesenquimatoso (MSC) (Padial-
Molina et al 2019) Las ceacutelulas se tomaron de donantes humanos sanos despueacutes de la aprobacioacuten
del Comiteacute de Eacutetica para la Investigacioacuten Humana de la Universidad de Granada (424 CEIH
2018) Medio de Eagle modificado por Dulbecco regular (DMEM) con 1 g L de glucosa
(DMEM-LG) (Gibco) suero bovino fetal al 10 (FBS) (Sigma-Aldrich St Louis MO EE UU)
1 100 de aminoaacutecidos no esenciales (NEAA) (Gibco) 001 μg ml de factor de crecimiento de
fibroblastos baacutesico (bFGF) (PeproTech Londres Reino Unido) 100 U ml de penicilina
estreptomicina y 025 μg ml de anfotericina B utilizado como medio de cultivo para todos los
experimentos Los cultivos se mantuvieron a 37 deg C en una atmoacutesfera de CO2 al 5 (2000 ceacutelulas
pocillo) Todos los experimentos bioloacutegicos se repitieron por triplicado al menos 3 veces por
condicioacuten
19Migracioacuten Celular
103
Un ensayo de migracioacuten celular se realizoacute como se describioacute anteriormente (Padial-Molina
Volk and Rios 2014) (Liang Park and Guan 2007) Brevemente las MSC se distribuyeron en
tres pocillos para cada condicioacuten y se les permitioacute crecer hasta una confluencia celular cercana al
99 en 24 pocillos placa de 3000 ceacutelulas cm2 y en cada pocillo se realizaron tres rasguntildeos
diferentes Luego las ceacutelulas se privaron de hambre durante 24 h mediante la adicioacuten de medio
de cultivo sin suero Se hizo un rasguntildeo usando una punta de pipeta a lo largo del diaacutemetro del
pozo Se realizoacute un paso de lavado con PBS para eliminar las ceacutelulas rayadas Se antildeadieron nuevos
medios de cultivo completos y se suplementaron seguacuten el grupo asignado (BMP-2 NP-BMP2 y
NP-BSA-BMP2 a 125 25 y 5 ng ml de BMP-2) Posteriormente se tomaron nueve imaacutegenes
de la misma aacuterea en cada condicioacuten hasta 48 h maacutes tarde En estas imaacutegenes el aacuterea raspada se
midioacute con el software ImageJ (Instituto Nacional de Salud Bethesda MD EUA
(httprsbwebnihgovij) La reduccioacuten en el aacuterea rayada con el tiempo se midioacute considerando el
aacuterea en el tiempo 0 como 100 abierta
20Proliferacioacuten celular
La proliferacioacuten se evaluoacute mediante un ensayo de sulforhodamina (SRB) (Houghton et al
2007) El ensayo se realizoacute sembrando las ceacutelulas a 1500 ceacutelulas cm2 en una placa de 96 pocillos
a una confluencia no superior al 50 Despueacutes de la unioacuten celular se agregaron los diferentes
suplementos (BMP-2 NP-BMP2 y NP-BSA-BMP2 a 125 25 y 5 ng ml de BMP-2) y las ceacutelulas
se mantuvieron en cultivo durante 7 diacuteas En cada punto de tiempo las ceacutelulas se lavaron con PBS
1X y se fijaron antildeadiendo aacutecido tricloroaceacutetico al 10 enfriado con hielo durante 20 minutos a
4ordmC Luego las ceacutelulas se lavaron 3 veces con dH2O y se secaron hasta que se recogieron todos
los puntos de tiempo Cada pocillo recibioacute 04 de SRB en 1 de aacutecido aceacutetico durante 20
minutos a temperatura ambiente con agitacioacuten suave La tincioacuten se terminoacute lavando cada pocillo
3 veces con aacutecido aceacutetico al 1 y secaacutendolo a temperatura ambiente durante 24 h El colorante se
recuperoacute de las ceacutelulas antildeadiendo Tris Base 10 mM a pH 105 y agitando suavemente durante 10
104
minutos La solucioacuten recuperada se distribuyoacute luego en una placa de 96 pocillos y se leyoacute la
absorbancia oacuteptica a 492 nm
bullDiferenciacioacuten osteogeacutenica
La diferenciacioacuten osteogeacutenica se evaluoacute mediante la adicioacuten de medios osteogeacutenicos al cultivo
celular en combinacioacuten con BMP-2 NP-BMP2 y NP-BSA-BMP2 libres a las dosis maacutes altas
utilizadas en experimentos anteriores Las ceacutelulas se sembraron a 3000 ceacutelulas cm2 y se
cultivaron para alcanzar una confluencia del 85 al 90 Esto fue seguido por la adicioacuten de
medios de induccioacuten que conteniacutean 10 mM de glicerofosfato (Fluka 50020) 01 μM de
dexametasona (Sigma-Aldrich D2915) y 005 mM de aacutecido L-ascoacuterbico (Sigma-Aldrich
A8960) Los cultivos celulares se mantuvieron durante 7 diacuteas para analizar la actividad temprana
En el diacutea 7 las ceacutelulas se recogieron en 1 ml de TRIzolreg Luego se extrajo el ARN y se convirtioacute
en ADNc Luego se evaluoacute la fosfatasa alcalina (ALP) y se calculoacute la expresioacuten con respecto a
la proteiacutena gliceraldehiacutedo-3-fosfato deshidrogenasa (GAPDH) por el meacutetodo 2 DDCt Estos
procedimientos se llevaron a cabo como describe en otra parte el siguiente autor (Padial-Molina
et al 2019) Las secuencias de cebador directo e inverso fueron
AGCTCATTTCCTGGTATGACAAC y TTACTCCTTGGAGGCCATGTG para GAPDH
TCCAGGGATAAAGCAGGTCTTG y CTTTCTCTTTCTCTGGCACTAAGG para ALP
bullEvaluacioacuten estadiacutestica
La migracioacuten y la proliferacioacuten celular se evaluaron mediante ANOVA seguido de la prueba
de comparaciones muacuteltiples de Tukey para el anaacutelisis por pares La comparacioacuten entre los niveles
de ALP a los 4 frente a los 7 diacuteas se analizoacute mediante un par de pruebas t de Student En todos los
casos se establecioacute un valor p inferior a 005 como significacioacuten estadiacutestica
105
53 Resultados y Discusioacuten
531Formulacioacuten de nanoparticulas
La evaporacioacuten de doble emulsioacuten-solvente ha sido descrita como un meacutetodo robusto y de uso
frecuente para producir NP de PLGA cargadas con biomoleacuteculas (Ding and Zhu 2018)
(McClements 2018) (Ortega-Oller et al 2015) (Iqbal et al 2015) Una formulacioacuten previamente
optimizada por nuestro grupo permitioacute la preservacioacuten de la actividad bioloacutegica de biomoleacuteculas
encapsuladas usando un solvente orgaacutenico ligeramente agresivo Ademaacutes el aacutecido desoxicoacutelico
se ha utilizado en el primer paso de la formulacioacuten para mejorar la estabilidad coloidal de las NP
y simultaacuteneamente para obtener superficies de NP enriquecidas con grupos carboxiacutelicos
mejorando su versatilidad y permitiendo que un quiacutemico posterior de lugar a la inmovilizacioacuten de
diferentes ligandos especiacuteficos (Sanchez-Moreno et al 2013) Por medio de esta formulacioacuten
mejorada en el presente trabajo desarrollamos nanopartiacuteculas vaciacuteas (NP) o nanopartiacuteculas que
encapsulan rhBMP-2 (NP-BMP2)
106
Una descripcioacuten esquemaacutetica del procedimiento de siacutentesis se muestra en la Figura 21
Figura 21 Esquema de la formulacioacuten de NP-BMP2
Para NP-BMP2 logramos una eficiencia de encapsulacioacuten de proteiacutenas (EE) de 97 plusmn 2 Este
resultado es estable en la literatura en la que varios autores han reportado valores igualmente altos
que encapsulan esta proteiacutena dentro de nanopartiacuteculas y micropartiacuteculas de PLGA (Lochmann et
al 2010) (Kempen et al 2008) Nuestra formulacioacuten tiene varios factores que conducen a este
elevado valor de EE La baja relacioacuten proteiacutena poliacutemero en masa (Manuel J Santander-Ortega
Csaba et al 2010) la afinidad de rhBMP-2 a una interaccioacuten inespeciacutefica con superficies
hidrofoacutebicas (Lochmann et al 2010) o la adicioacuten de estabilizadores (poloxaacutemero) en el segundo
paso del procedimiento de doble emulsioacuten (Ortega-Oller et al 2015) La ausencia de rhBMP-2
en el sobrenadante resultante de la etapa de centrifugacioacuten en el proceso de limpieza se verificoacute
mediante ELISA y SDS-PAGE en el que se muestra una banda clara correspondiente a 14 kD de
cadenas polipeptiacutedicas rhBMP-2 para el carril A en la Figura 22 correspondiente a NP-BMP2
La masa de proteiacutena encapsulada alrededor de 2 microg es similar a la de diferentes micro y
107
nanosistemas PLGA descritos en la literatura (Wang et al 2015) (Chung et al 2007) (La et al
2010) Teniendo en cuenta las condiciones de almacenamiento para nuestras muestras esto
corresponde a 500 ng mL lo que representa una cantidad suficiente de concentracioacuten para
aplicaciones praacutecticas ya que este factor de crecimiento muestra actividades bioloacutegicas in vitro en
dosis muy bajas (5ndash20 ng ml) (Ortega-Oller et al 2015)
Figura 22 Anaacutelisis de electroforesis en gel de SDS-poliacrilamida (SDS-PAGE) en
condiciones reductoras de Nanopartiacuteculas de PLGA soacutelidas (NP de PLGA) y fracciones liacutequidas
(sobrenadante) de diferentes sistemas de NP Carril P proteiacutenas estaacutendares carril A NP-BMP2
(proteiacutena morfogeneacutetica oacutesea) carril B sobrenadante de NP-BMP2 despueacutes de la siacutentesis y
encapsulacioacuten de rhBMP-2 carril C NP despueacutes de la adsorcioacuten fiacutesica de BSA rhBMP-2 carril
D sobrenadante despueacutes de la adsorcioacuten fiacutesica de BSA (albuacutemina de suero bovino) rhBMP-2
en el sistema NP
Por otro lado resultoacute un segundo nanosistema modificando la forma en que rhBMP-2 es
incorporado en el nanoportador Hay varios ejemplos de adsorcioacuten superficial de diferentes
factores de crecimiento en micro y nanopartiacuteculas (La et al 2010) (Fu et al 2012) (Rahman et
al 2014) y la inmovilizacioacuten de la superficie sobre la encapsulacioacuten recientemente se ha
108
propuesto como una forma de modular la liberacioacuten posterior de biomoleacuteculas Este proceso que
depende de la lenta difusioacuten de las biomoleacuteculas a traveacutes de la matriz polimeacuterica estaacute en
consecuencia altamente influenciado por la interaccioacuten proteiacutena-poliacutemero (Pakulska et al 2016)
(Fu et al 2017) y degradacioacuten del poliacutemero (Mir Ahmed and Rehman 2017) (Ding and Zhu
2018) Por lo tanto este nuevo enfoque en el uso de NP de PLGA para el suministro de
biomoleacuteculas se exploroacute inmovilizando la proteiacutena rhBMP-2 en la superficie de las NP vaciacuteas
mediante una simple adsorcioacuten fiacutesica Se sabe que este proceso se rige por interacciones
electrostaacuteticas e hidrofoacutebicas entre las moleacuteculas de proteiacutenas y las superficies NP (Peula and de
las Nieves 1993)
Para esto los grupos cargados en la superficie la hidrofilia la carga neta de las moleacuteculas de
proteiacutena y las caracteriacutesticas del medio de adsorcioacuten son los paraacutemetros de referencia Por lo
tanto disentildeamos un experimento de co-adsorcioacuten en el que interactuacutea una mezcla de rhBMP-2 y
BSA (04 p p rhBMP-2 BSA) simultaacuteneamente con la superficie de NP de PLGA Las
albuacuteminas se usan habitualmente como proteiacutenas protectoras cuando los factores de crecimiento
se incorporan en las NPs de PLGA (Ortega-Oller et al 2015) (Zhang et al 2016) Ademaacutes una
distribucioacuten superficial de las moleacuteculas de BSA puede mejorar la estabilidad coloidal de las NP
a pH fisioloacutegico debido a su carga negativa neta bajo estas condiciones (Peula and de las Nieves
1994) La Figura 23 muestra un esquema del proceso de co-adsorcioacuten La eficiencia de adsorcioacuten
es superior al 95 y en el SDS-PAGE de la Figura 22 se pueden ver dos bandas caracteriacutesticas
de ambas proteiacutenas en el carril C correspondiente al nanosistema NP-BSA-BMP2
109
Figura 23 Esquema del proceso de adsorcioacuten de proteiacutenas para NP-BSA-BMP2
Sin embargo el carril D correspondiente a la ejecucioacuten del sobrenadante desde la
centrifugacioacuten del nanosistema despueacutes de los procesos de adsorcioacuten muestra la ausencia de
cualquier proteiacutena Este resultado se explica completamente teniendo en cuenta el pH del medio
(pH 50) cerca del punto isoeleacutectrico de BSA donde la adsorcioacuten de esta proteiacutena en
nanopartiacuteculas cargadas negativamente presenta un maacuteximo (Peula and de las Nieves 1993)
(Peula Hidalgo-Alvarez and de las Nieves 1995) La inmovilizacioacuten de rhBMP-2 en la superficie
cargada negativamente de las NPs demuestra que estaacuten favorecidos electrostaacuteticamente debido a
la carga neta positiva de esta proteiacutena a pH aacutecido y neutro
532Caracterizacioacuten de nanopartiacuteculas
21Tamantildeo de nanopartiacuteculas
Micrografiacuteas SEM y STEM (Figura 24) muestran que las muestras consisten en partiacuteculas
esfeacutericas de diaacutemetros diferentes (entre 150 y 450 nm) un rango similar al encontrado en un
trabajo anterior en el que las NP se cargaron con lisozima siguiendo un protocolo de siacutentesis
similar (Ortega-Oller et al 2017) En ese trabajo la teacutecnica DLS no pudo proporcionar una
distribucioacuten de tamantildeo confiable Por lo tanto la teacutecnica NTA fue directamente utilizada para
determinar el tamantildeo hidrodinaacutemico de las NP cargadas con BMP2
Las distribuciones de tamantildeo para NP vaciacuteas (NP) y cargadas con BMP (NP-BMP2) de NTA
(Figura 25 y videos S1 S2) fueron consistentes con las imaacutegenes SEM Se encontroacute que las
partiacuteculas con diaacutemetros entre 100 y 500 nm teniacutean la concentracioacuten de partiacuteculas maacutes alta en
110
alrededor de 200 nm La carga con BMP tuvo un efecto en la distribucioacuten del tamantildeo lo que
condujo a picos maacutes definidos Estas mediciones nos permitieron determinar la concentracioacuten de
partiacuteculas en la muestra medida 688 plusmn 009 x 10 8 pp mL y 519 plusmn 012 x10 8 pp mL para
nanosistemas NP y NP-BMP2 respectivamente Estos valores fueron utilizados (teniendo en
cuenta la dilucioacuten correspondiente) para controlar el nuacutemero de partiacuteculas antildeadidas en los
experimentos celulares
Figura 24 Micrografiacutea de microscopiacutea electroacutenica de barrido (SEM) de nanopartiacuteculas
cargadas con rhBMP-2 (NP-BMP2)
111
Figura 25 Distribucioacuten del diaacutemetro hidrodinaacutemico de NP (ciacuterculos) y NP-BMP2 (liacutenea
negra gruesa) medidos a pH 70 (tampoacuten de fosfato) por anaacutelisis de seguimiento de
nanopartiacuteculas (NTA)
bullMovilidad electrocineacutetica y estabilidad coloidal
La carga superficial de las nanopartiacuteculas se puede analizar mediante un estudio electrocineacutetico
midiendo la movilidad electroforeacutetica (microe) en diferentes condiciones La Figura 26 muestra la
movilidad electroforeacutetica y el potencial zeta evaluado para los tres nanosistemas NP NP- BMP2
y NP-BSA-BMP2 a baja fuerza ioacutenica y diferentes valores de pH La carga superficial eleacutectrica
de las NP reside en los grupos carboxiacutelicos de las no cubiertas por PLGA y moleacuteculas de aacutecido
desoxicoacutelico Estos grupos funcionalizados tambieacuten son uacutetiles debido a la posibilidad de una
vectorizacioacuten quiacutemica de la superficie para desarrollar nanoportadores de entrega dirigida
(Siafaka et al 2016)
Se confirmoacute previamente que la protonacioacuten de estos grupos de superficies aacutecidas a valores de
pH bajo su valor de pKa estaba estrechamente relacionado con una peacuterdida de carga superficial
y en consecuencia una reduccioacuten (en valor absoluto) de la movilidad electroforeacutetica del sistema
coloidal (Peula-Garciacutea Hidalgo-Alvarez and De Las Nieves 1997) (Manuel J Santander-Ortega
Lozano-Loacutepez et al 2010) Por lo general cuando las partiacuteculas coloidales estaacuten recubiertas por
Conce
ntr
acioacute
n d
e par
tiacutecu
las
(10
6 p
pm
L)
112
moleacuteculas de proteiacutena los valores de microe cambian notablemente en comparacioacuten con el mismo
valor de las superficies desnudas y estaacuten influenciadas por la carga eleacutectrica de las moleacuteculas de
proteiacutena adsorbidas (Peula-Garcia Hidaldo-Alvarez and De las Nieves 1997) (Santander-Ortega
Bastos-Gonzalez and Ortega-Vinuesa 2007) El comportamiento electrocineacutetico del sistema NP-
BMP2 sigue siendo similar al de NP y la encapsulacioacuten de rhBMP-2 no afecta la distribucioacuten de
carga superficial DAngelo y colaboradores informaron de un resultado similar al encapsular
diferentes factores de crecimiento en nanopartiacuteculas de mezcla de PLGA-poloxaacutemero en la
misma proporcioacuten p p de proteiacutena poliacutemero (drsquoAngelo et al 2010) Esto puede deberse a la
baja cantidad de proteiacutena encapsulada y su distribucioacuten en la parte interna de las NP (lejos de la
superficie) En nuestro sistema la distribucioacuten interna puede verse favorecida por las condiciones
de encapsulacioacuten donde el pH baacutesico (pH 120) del agua contiene rhBMP-2 que permite una carga
negativa de estas moleacuteculas de proteiacutena evitando asiacute su interaccioacuten electrostaacutetica especiacutefica con
grupos aacutecidos de las NP
113
Figura 26 Movilidad electroforeacutetica y potencial zeta versus pH en medios de baja salinidad
(fuerza ioacutenica igual a 0002 M) para los diferentes nanosistemas (cuadrado negro) NP
(triaacutengulo azul) NP-BMP2 (ciacuterculo rojo) NP-BSA-BMP2
La distribucioacuten electrocineacutetica para el sistema NP-BSA-BMP2 cambia radicalmente Como se
mostroacute anteriormente la eficiencia de adsorcioacuten muy alta conduce a NP con ambas proteiacutenas
adsorbidas alrededor de su superficie Esta situacioacuten estaacute estrechamente relacionada con los
valores microe de la Figura 26 Teniendo en cuenta la relacioacuten p p entre proteiacutenas adsorbidas (250
veces mayor para BSA) las moleacuteculas de albuacutemina modulan el comportamiento a valores de pH
por debajo de su punto isoeleacutectrico (pI 47) donde la carga neta positiva BSA enmascara la carga
superficial original de las NP e incluso cambia sus valores originales a valores positivos Esto es
un resultado tiacutepico encontrado para estas partiacuteculas coloidales que cubren proteiacutenas (Peula
Hidalgo-Alvarez and de las Nieves 1995) (Peula JM Callejas J de las Nieves 1994) A
valores de pH neutros y baacutesicos las moleacuteculas de BSA tienen una carga neta negativa y la ligera
disminucioacuten en los valores absolutos microe podriacutea ser debido a la reduccioacuten de la carga superficial
neta negativa de los NP que pueden estar protegidos al menos en una pequentildea parte por la carga
positiva de las moleacuteculas de rhBMP-2 bajo su punto isoeleacutectrico baacutesico (pI 90)
Pote
nci
al z
eta
(mV
)
114
La estabilidad coloidal para los diferentes nanosistemas (NP NP-BMP2 y NP-BSA-BMP2)
fue determinada mediante el anaacutelisis de las distribuciones de tamantildeo en varios medios (PB PBS
y DMEM) en diferentes tiempos despueacutes de la siacutentesis (0 1 y 5 diacuteas) Se encontraron
distribuciones de tamantildeo similares a las originales para las dos formulaciones NP y NP-BMP2
en todos los medios analizados Este resultado fue similar al encontrado previamente para estos
tipos de NP que encapsulan la lisozima (Ortega-Oller et al 2017) en el que la combinacioacuten de
las interacciones electrostaacuteticas y esteacutericas generadas por grupos quiacutemicos superficiales de NP
confieren estabilidad al mecanismo que evita la agregacioacuten coloidal (Manuel J Santander-Ortega
Csaba et al 2010) La disminucioacuten del valor absoluto del potencial zeta para el sistema NP-
BSA-BMP2 como consecuencia de la distribucioacuten de proteiacutenas en la superficie no afecta su
estabilidad coloidal Este sistema tambieacuten mantiene la misma distribucioacuten de tamantildeos en los
diferentes medios Se acepta comuacutenmente que un potencial zeta superior a +30 o 30 mV daraacute
lugar a un sistema coloidal estable (Sun 2016) y el valor potencial zeta para NP-BSA-BMP2 es
superior a 30 mV La estabilidad coloidal en PBS y DMEM tiacutepicamente medios utilizados para
el desarrollo de interacciones celulares o scaffolds respectivamente asegura el uso potencial de
estos nanosistemas para entornos de vida in vitro o in vivo Ademaacutes estos sistemas mantuvieron
su tamantildeo bajo almacenamiento en PB a 4 deg C durante al menos 1 mes (datos no mostrados) lo
que demuestra que es un medio adecuado para el almacenamiento de muestras
bullLiberacioacuten de proteiacutenas
Uno de los principales problemas para los micro o nanosistemas de suministro de faacutermacos
PLGA es encontrar el patroacuten de liberacioacuten apropiado para las moleacuteculas de proteiacutenas encapsuladas
unidas Un amplio espectro de formulaciones modula esta propiedad mediante el uso de
diferentes tipos de procesos de siacutentesis poliacutemeros PLGA copoliacutemeros y estabilizadores (Mir
Ahmed and Rehman 2017) (Ortega-Oller et al 2015) Una limitacioacuten y control adecuados en la
liberacioacuten de estallido es fundamental para las BMP a fin de garantizar una liberacioacuten continua a
largo plazo que favorecida por la degradacioacuten del poliacutemero proporcione una mejor accioacuten in vivo
115
para impulsar la regeneracioacuten de hueso y cartiacutelago (Begam et al 2017) Por lo tanto previamente
desarrollamos un nanosistema PLGA dual para la liberacioacuten controlada a corto plazo donde la
difusioacuten de proteiacutenas y la interaccioacuten proteiacutena-poliacutemero son los principales factores que rigen este
proceso (Ortega-Oller et al 2017) En el presente trabajo los nanosistemas NP-BMP2 y NP-
BSA-BMP2 representan dos formas diferentes en las que se incorporoacute rhBMP-2 en el
nanoportador La Figura 27a muestra la liberacioacuten acumulativa de ambas proteiacutenas rhBMP-2 y
BSA para diferentes sistemas en funcioacuten del tiempo en un periacuteodo a corto plazo (7 diacuteas) La
proteiacutena rhBMP-2 encapsulada alcanza una cantidad liberada de alrededor del 30 de la proteiacutena
encapsulada inicial mientras que la rhBMP-2 adsorbida a pesar de su distribucioacuten superficial es
tres veces menor Sin embargo BSA muestra cantidades liberadas de hasta el 80 de los
adsorbidos iniciales En todos los casos las barras de error corresponden a las desviaciones
estaacutendar de tres experimentos independientes En estas condiciones el factor de crecimiento
encapsulado en NP-BMP2 presenta un patroacuten de liberacioacuten similar al encontrado previamente con
la misma formulacioacuten pero usando lisozima como proteiacutena (Ortega-Oller et al 2017) El
poloxaacutemero en la fase acuosa del proceso de siacutentesis puede ser clave para modular las
interacciones proteicas interfaciales especiacuteficas y no especiacuteficas (Del Castillo-Santaella et al
2019) Por lo tanto la relacioacuten entre la interaccioacuten proteiacutena-poliacutemero y la difusioacuten de proteiacutenas
parece estar bien equilibrada evitando un estallido inicial excesivo y al mismo tiempo
manteniendo el flujo de proteiacutenas necesario para liberar alrededor de un tercio de la rhBMP-2
encapsulada en 7 diacuteas Aunque se ha informado ampliamente de un estallido inicial excesivo para
los NP de PLGA relacionados con moleacuteculas de proteiacutenas cercanas a la superficie (Ding and Zhu
2018) esta situacioacuten no aparecioacute para el sistema NP-BMP2 siendo esto consistente con el
comportamiento electrocineacutetico que no mostroacute la presencia de proteiacutena cerca de la superficie La
literatura ofrece algunos ejemplos con liberacioacuten reducida a corto plazo de BMP-2 utilizando maacutes
copoliacutemeros PLGA-PEG hidroacutefilos (Kirby et al 2011) o un proceso de siacutentesis diferente (Chang
et al 2017)
116
El rendimiento de la liberacioacuten del sistema NP-BSA-BMP2 que tambieacuten se muestra en la
Figura 27a presenta diferencias notables El perfil electrocineacutetico ha justificado previamente la
ubicacioacuten de la superficie de BSA y rhBMP-2 en la superficie lo que podriacutea conducir a una
liberacioacuten raacutepida de ambas proteiacutenas Sin embargo los resultados de la Figura 27a y 27b muestran
esta tendencia solo para la proteiacutena BSA que se libera de las NP con aproximadamente el 20
de la cantidad inicial restante despueacutes de siete diacuteas Sin embargo hasta el 90 de la carga inicial
de proteiacutena rhBMP-2 a diferencia de BSA permanece unida a la superficie La superficie NP con
grupos hidrofiacutelicos forma moleacuteculas de poloxaacutemero y una carga negativa debido a la abundante
presencia de grupos carboxiacutelicos (grupos terminales de PLGA y moleacuteculas de aacutecido desoxicoacutelico)
favorecen un proceso de desorcioacuten para BSA cuyas moleacuteculas tienen una carga negativa en
condiciones de liberacioacuten (pH fisioloacutegico) Esto concuerda con los resultados de otros autores
que incluso despueacutes de encapsular BSA en mezclas de PLGA-poloxaacutemero NP logroacute una
descarga de liberacioacuten raacutepida por encima del 40 o 50 de la cantidad inicial de proteiacutena (Manuel
J Santander-Ortega Csaba et al 2010) Ademaacutes la coencapsulacioacuten de albuacuteminas con factores
de crecimiento podriacutea afectar fuertemente su perfil de liberacioacuten causando un estallido inicial
(Balmayor et al 2009) (drsquoAngelo et al 2010) De lo contrario la atraccioacuten electrostaacutetica
especiacutefica entre las moleacuteculas rhBMP-2 positivas y los grupos de superficie negativos ralentiza la
liberacioacuten a corto plazo de esta proteiacutena Este resultado estaacute de acuerdo con la baja liberacioacuten de
BMP adsorbida encontrada previamente usando micro y nanopartiacuteculas de PLGA con grupos
terminales de aacutecido sin tapar (Pakulska et al 2016) (Schrier and DeLuca 2001) Por lo tanto la
combinacioacuten de diferentes meacutetodos para atrapar BMP-2 dentro y alrededor de NP muestra la
posibilidad de lograr una liberacioacuten controlada adecuadamente equilibrando las interacciones
entre poliacutemeros estabilizadores y proteiacutenas
117
(a)
(b)
Figura 27 (a) Liberacioacuten acumulativa de rhBMP-2 para sistemas NP-BMP2 (cuadrado
negro) y NP-BSA-BMP2 (ciacuterculo rojo) y liberacioacuten acumulativa de BSA para el sistema NP-
BSA-BMP2 (triaacutengulo azul) incubado por diferentes tiempos a 37ordmC en tampoacuten de fosfato salino
(pH 74) (b) Anaacutelisis de SDS-PAGE en condiciones reductoras de fraccioacuten soacutelida de NP-BSA-
Lib
erac
ioacuten a
cum
ula
tiva
()
Tiempo (horas)
118
BMP2 despueacutes de la liberacioacuten en diferentes momentos en los que el nuacutemero de cada carril
corresponde al tiempo en horas
533Actividad bioloacutegica e interacciones
bullMigracioacuten Celular
La migracioacuten celular es el primer y necesario paso en la regeneracioacuten de tejidos (Padial-Molina
OrsquoValle et al 2015) Por lo tanto un agente regenerativo debe acelerar la migracioacuten celular o
al menos no interferir con ella En el presente estudio no encontramos diferencias entre los
grupos las dosis y el control en teacuterminos de cierre de un aacuterea rayada (ANOVA con la prueba de
comparaciones muacuteltiples de Tukey) (Figura 28) En contraste con nuestros hallazgos los datos
publicados anteriormente sugieren un efecto positivo de BMP-2 en la migracioacuten celular (Inai et
al 2008) (Gamell et al 2008) Sin embargo en esos estudios las dosis aplicadas y los tipos de
ceacutelulas fueron diferentes a los experimentos actuales Utilizamos dosis maacutes bajas de BMP-2 para
evaluar si incluso a dosis bajas BMP-2 podriacutea proporcionar beneficios si se protegiera en un
sistema de nanopartiacuteculas Como se mencionoacute no demostramos ninguacuten efecto negativo del
sistema en la migracioacuten celular Sin embargo nuestros resultados respaldan la idea de que la
actividad de BMP-2 estaacute mediada por la activacioacuten de la viacutea de la fosfoinositida 3-quinasa (PI3K)
un grupo comuacuten de moleacuteculas de sentildealizacioacuten que participan en varios procesos con BMP-2 y
otras moleacuteculas (Padial-Molina Volk and Rios 2014) (Gamell et al 2008) Tambieacuten debe
mencionarse que el plazo de un ensayo de migracioacuten es corto Por lo tanto las ventajas potenciales
de un sistema de liberacioacuten controlada como el que se estaacute estudiando podriacutean ser limitadas Es
decir la liberacioacuten de BMP-2 de las nanopartiacuteculas como se demuestra en la Figura 27 se limita
a las primeras 48 h Por lo tanto se podriacutea hipotetizar un efecto positivo sostenido sobre la
actividad migratoria a lo largo del tiempo
119
Figura 28 Ensayo de migracioacuten Porcentaje de cierre del aacuterea rayada a las 24 y 48 h en
diferentes grupos y dosis
bullProliferacioacuten celular
La proliferacioacuten es otra de las actividades celulares requeridas para la regeneracioacuten de tejidos
Sin embargo esta propiedad debe equilibrarse con la migracioacuten y la diferenciacioacuten y no aumentar
las tres caracteriacutesticas al mismo tiempo y con las mismas proporciones (Friedrichs et al 2011)
De hecho seguacuten los informes cuando una dosis de BMP-2 induce una mayor proliferacioacuten
disminuye la diferenciacioacuten (Hrubi et al 2018) Esta propiedad ha sido ampliamente analizada
pero las discrepancias auacuten se pueden detectar en la literatura Por lo tanto Kim y colaboradores
analizoacute diferentes dosis de BMP-2 y su efecto sobre la proliferacioacuten celular y la apoptosis Se
confirmoacute in vitro que las dosis altas pero auacuten maacutes bajas que las utilizadas cliacutenicamente reducen
la proliferacioacuten celular y aumentan la apoptosis (Kim Oxendine and Kamiya 2013) Esto debe
ser evitado Hemos encontrado que aunque el BMP-2 libre no induce una proliferacioacuten mayor
que el control en ninguna de las dosis aplicadas ni en los puntos de tiempo (ANOVA con la prueba
0
10
20
30
40
50
60
70
80
90
100
0 h 24 h 48 h
o
f sc
ratc
hed
are
a
Time point
Control
BMP2-125 ngmL
BMP2-25 ngmL
BMP2-5 ngmL
NP-BMP2-125 ngmL
NP-BMP2-25 ngmL
NP-BMP2-5 ngmL
NP-BSA-BMP2-125 ngmL
NP-BSA-BMP2-25 ngmL
NP-BSA-BMP2-5 ngmL
d
e aacuter
ea r
ayad
a
Tiempo
120
de comparaciones muacuteltiples de Tukey) la misma cantidad de BMP-2 encapsulada o adsorbida en
nanopartiacuteculas de PLGA aumenta la proliferacioacuten siendo esto estadiacutesticamente significativo
cuando se usa una dosis de 25 ng mL o maacutes (ANOVA con prueba de comparaciones muacuteltiples
de Tukey) (Figura 29) Estas dosis son auacuten maacutes bajas que las sugeridas en estudios anteriores
Aparte de esa diferencia todaviacutea se logroacute un efecto positivo sobre la proliferacioacuten Ademaacutes
siguiendo el patroacuten de lanzamiento de la Figura 27 se espera que se libere maacutes BMP-2 con el
tiempo maacutes allaacute del marco de tiempo de 7 diacuteas Por lo tanto tambieacuten podriacutea esperarse un efecto
de induccioacuten sostenido hasta la confluencia completa del cultivo celular
Figura 29 Proliferacioacuten de ceacutelulas del estroma mesenquimatoso humano (MSC) medida por
absorbancia de sulforamida (SRB) Los resultados se normalizaron a T0 en cada grupo
T0 T1 T2 T3 T4 T6 T70
2
4
6
Time point
No
rmalized
Ab
so
rban
ce
NP-BSA-BMP2-125 ngmL
NP-BSA-BMP2-25 ngmL
NP-BSA-BMP2-5 ngmL
NP-BMP2-125 ngmL
NP-BMP2-25 ngmL
NP-BMP2-5 ngmL
BMP2-125 ngmL
BMP2-25 ngmL
BMP2-5 ngmL
Control
Abso
rban
cia
norm
aliz
ada
Tiempo
121
bullDiferenciacioacuten osteogeacutenica
Se ha confirmado que la diferenciacioacuten celular inducida por BMP-2 necesita la presencia de
componentes osteoinductores permisivos En particular se ha demostrado que el beta-
glicerofosfato ejerce un efecto sineacutergico con BMP-2 para inducir la diferenciacioacuten celular (Hrubi
et al 2018) Por lo tanto para probar la diferenciacioacuten osteogeacutenica analizamos la expresioacuten del
ARNm de ALP Se encontroacute que la actividad maacutexima de ALP se produce 10 diacuteas despueacutes de la
estimulacioacuten con micropartiacuteculas basadas en PLGA que contienen BMP-2 en coencapsulacioacuten
con albuacutemina seacuterica humana (Kirby et al 2011) Aunque otras pruebas podriacutean haberse utilizado
para reforzar nuestros hallazgos se sabe que ALP modula la deposicioacuten de noacutedulos mineralizados
lo que indica actividad osteoblaacutestica Para todo esto complementamos los medios de
diferenciacioacuten con Beta-glicerofosfato y BMP-2 NP-BMP2 o NP-BSA-BMP2 libres durante 4 y
7 diacuteas para poder capturar la dinaacutemica temprana de la expresioacuten del gen En nuestro estudio
identificamos un aumento en la expresioacuten de ALP en todos los grupos desde el diacutea 4 hasta el diacutea
7 (Figura 30) Aunque ALP en el diacutea 7 en el grupo BMP-2 parece ser maacutes alto que en los otros
dos grupos el cambio no resultoacute significativo De hecho las diferencias entre los grupos no fueron
estadiacutesticamente significativas en ninguacuten periacuteodo de tiempo Sin embargo cabe destacar que el
aumento no fue significativo dentro del grupo BMP-2 (p = 0141 prueba t de Student) pero fue
significativo dentro de los otros dos grupos (p = 0025 y p = 0003 NP-BMP2 y NP-BSA- Grupos
BMP2 respectivamente) Esto nuevamente podriacutea tomarse como una confirmacioacuten de la
liberacioacuten sostenida de la proteiacutena del sistema de nanopartiacuteculas maacutes allaacute de los puntos temporales
anteriores
Esto y los estudios de migracioacuten y proliferacioacuten descritos a continuacioacuten nos llevan a confirmar
que el sistema propuesto puede mantener una liberacioacuten adecuada de BMP-2 a lo largo del tiempo
manteniendo un efecto positivo en la migracioacuten y proliferacioacuten celular con dosis iniciales
reducidas de BMP-2 El hecho de que se evite el estallido inicial excesivo es importante para la
aplicacioacuten de esta nanotecnologiacutea en la regeneracioacuten oacutesea como en la odontologiacutea De esta
122
manera los efectos negativos de las altas dosis iniciales de BMP-2 se evitan al mismo tiempo que
la moleacutecula estaacute protegida contra la desnaturalizacioacuten dentro del NP Por lo tanto los efectos del
regenerador se mantienen con el tiempo Los experimentos in vitro mostraron que las NP de
PLGA cargadas con BMP-2 son los nanoportadores con el mejor perfil de liberacioacuten a corto plazo
sin una explosioacuten inicial y con una liberacioacuten moderada y sostenida de proteiacutena activa antes del
inicio de la degradacioacuten del poliacutemero Por lo tanto la actividad bioloacutegica es positiva sin
interaccioacuten negativa con la migracioacuten o la proliferacioacuten sino maacutes bien la induccioacuten de la
diferenciacioacuten celular a traveacutes de la expresioacuten de ALP
Figura 30 Cambio de pliegue relativo en la expresioacuten de ARNm de ALP (grupo de control
BMP2 a los 4 diacuteas) = Importancia estadiacutestica de la comparacioacuten a lo largo del tiempo (p =
0025 y p = 0003 prueba t del estudiante grupos NP-BMP2 y NP-BSA-BMP2)
Los experimentos de liberacioacuten in vitro muestran un patroacuten adecuado de administracioacuten a corto
plazo que al mismo tiempo preserva la bioactividad de la biomoleacutecula encapsulada Ademaacutes la
distribucioacuten de tamantildeo de nanopartiacutecula encontrada para este nanosistema W-F68 permite la
Tiempo
Cam
bio
de
pli
egue
123
posibilidad de una administracioacuten de proteiacutena dual externa e intracelular como se ha demostrado
mediante experimentos celulares in vitro Esta nueva formulacioacuten se utilizaraacute en futuros estudios
para encapsular y administrar factores de crecimiento in vitro e in vivo con el fin de explotar el
potencial terapeacuteutico de este nanosistema
Se ha puesto de manifiesto la necesidad de optimizacioacuten de los meacutetodos y componentes para
equilibrar la estructura y morfologiacutea de las micropartiacuteculas nanopartiacuteculas de PLGA logrando
de esta forma una alta eficiencia de encapsulacioacuten de BMP-2 y buscando un objetivo principal el
control de la entrega la reduccioacuten de la descarga inicial para alcanzar un perfil de liberacioacuten de
la proteiacutena sostenido en el tiempo preservando la actividad bioloacutegica y dirigieacutendola a ceacutelulas
diana para minimizar la cantidad cliacutenica de proteiacutena necesaria permitiendo al mismo tiempo una
correcta regeneracioacuten del tejido oacuteseo
En consecuencia otro reto futuro es conseguir el direccionamiento especiacutefico de estas nano-
esferas de PLGA cargadas de agentes activos Este aspecto se puede desarrollar mediante el uso
de unos ligandos que reconozcan especiacuteficamente los tipos o liacuteneas celulares a la que queremos
dirigir la liberacioacuten de biomoleacuteculas encapsuladas El uso de nanopartiacuteculas con una unioacuten
covalente de diferentes ligandos da lugar a una teacutecnica con un alto potencial de administracioacuten
que permite a la ingenieriacutea tisular un gran avance en cuanto a la distribucioacuten y administracioacuten de
diferentes faacutermacos o biomoleacuteculas mejorando asiacute las funciones bioloacutegicas o regenerativas
celulares
Los anticuerpos especiacuteficos que reconocen los receptores de superficie celular pueden unirse
covalentemente a la superficie de nuestras nanopartiacuteculas de PLGA dando lugar a una ldquoinmuno-
nanopartiacuteculardquo Para que esta unioacuten se produzca sin ninguacuten inconveniente en muchas ocasiones
hay que hacer uso de agentes estabilizadores para proporcionar estabilidad coloidal a las
nanopartiacuteculas sin que lleguen a afectar al enlace establecido entre los anticuerpos especiacuteficos de
los receptores celulares y las nanopartiacuteculas que es donde nos encontramos hoy diacutea y donde
124
muchos investigadores siguen haciendo avances cada diacutea entorno a la mejora de estos enlaces y
de estas entregas celulares especificas a traveacutes de las ldquoinmuno-nanopartiacuteculasrdquo
125
6CONCLUSIONES
En respuesta al objetivo principal de este trabajo
Los nanosistemas de partiacuteculas polimeacutericas basados en PLGA son sistemas prometedores para la
administracioacuten espacial y temporalmente controlada de factores de crecimiento que promueven
el desarrollo celular la diferenciacioacuten y regeneracioacuten en ingenieriacutea oacutesea mediante su
incorporacioacuten junto a las ceacutelulas en estructuras soacutelidas o hidrogeles
En respuesta a los objetivos secundarios de este trabajo
bullHa sido posible optimizar la obtencioacuten de diferentes sistemas de NPs de PLGA mediante un
procedimiento de doble emulsioacuten con las propiedades superficiales adecuadas que proporcionan
estabilidad coloidal y grupos carboxilo superficiales para unir quiacutemicamente diferentes ligandos
especiacuteficos en respuesta al objetivo 1
bullSe ha optimizado una formulacioacuten W-F68 que basada en el procedimiento anterior permite
encapsular moleacuteculas hidrofiacutelicas como las proteiacutenas obteniendo un novedoso nanotransportador
de tamantildeo dual que preserva la actividad bioloacutegica de la proteiacutena modelo encapsulada (lisozima)
en respuesta al objetivo 2
bullEn respuesta al tercer objetivo el anaacutelisis de la interaccioacuten proteiacutena-surfactante muestra el
papel crucial del solvente orgaacutenico el surfactante la relacioacuten de volumen entre ambas fases y la
carga neta de la proteiacutena encapsulada sobre las caracteriacutesticas finales de las NPs transportadoras
y el patroacuten de liberacioacuten proteica
bullLas experiencias in vitro de suministro proteico muestran una difusioacuten bien equilibrada de la
proteiacutena evitando una descarga inicial excesiva manteniendo un flujo constante de liberacioacuten a
corto plazo y permitiendo un suministro dual extra e intracelular sin citotoxicidad apreciable en
respuesta a los objetivos 4 y 5
126
bullSe ha desarrollado un nanosistema transportador de BMP2 sobre la base de un sistema modelo
formulado previamente mediante un procedimiento de doble emulsioacuten que conduce a un sistema
de NPs con una distribucioacuten dual de tamantildeos y estabilidad coloidal y temporal adecuada para
aplicaciones bioloacutegicas en respuesta al objetivo 6
bullIn vitro el Sistema con BMP2 encapsulada presenta un patroacuten de liberacioacuten en el corto plazo
7 diacuteas que muestra un suministro moderado y sostenido de proteiacutena bioloacutegicamente activa en
respuesta al objetivo 7
bullIn vitro la actividad bioloacutegica a nivel celular muestra mediante el anaacutelisis de la expresioacuten de
ALP la capacidad de la BMP2 nanotransportada para inducir diferenciacioacuten celular sin incidencia
negativa en los procesos de migracioacuten y proliferacioacuten celular en respuesta al objetivo 8
127
7 CONFLICTO DE INTERESES
Los autores declaran no tener ninguacuten conflicto de intereses con ninguno de los productos
enumerados en el documento
8 RECURSOS ECONOacuteMICOS
Beca de investigacioacuten obtenida competitivamente y otorgada por la empresa de implantes
dentales ldquoMIS IBERICA SLrdquo
Financiacioacuten parcial otorgada 1- por la Consejeriacutea de Economiacutea Innovacioacuten Educacioacuten
Ciencia y Empleo de la Junta de Andaluciacutea (Espantildea) 2- los proyectos MAT2013-43922-R ndash
incluyendo soporte europeo FEDER - (MICINN Espantildea) 3- y los Grupos de Investigacioacuten
FQM-115 CTS-1028 CTS-138 y CTS- 583 (Junta de Andaluciacutea Espantildea)
128
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Chang H-C et al (2017) lsquoBone morphogenetic protein-2 loaded poly(DL-lactide-co-
glycolide) microspheres enhance osteogenic potential of gelatinhydroxyapatitebeta-
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Chen G Deng C and Li Y-P (2012) lsquoTGF-beta and BMP signaling in osteoblast
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Cheng J et al (2007) lsquoFormulation of functionalized PLGA-PEG nanoparticles for in vivo
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Csaba N et al (2005) lsquoPLGApoloxamer and PLGApoloxamine blend nanoparticles new
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Csaba N Garcia-Fuentes M and Alonso M J (2006) lsquoThe performance of nanocarriers for
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from particle preparation to temperature-triggered aggregationrsquo Langmuir the ACS
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based drug delivery systems--a reviewrsquo International journal of pharmaceutics 415(1ndash2)
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Froum S J et al (2006) lsquoComparison of mineralized cancellous bone allograft (Puros) and
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Fu C et al (2017) lsquoEnhancing Cell Proliferation and Osteogenic Differentiation of MC3T3-
E1 Pre-osteoblasts by BMP-2 Delivery in Graphene Oxide-Incorporated PLGAHA
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Gaudana R et al (2013) lsquoDesign and evaluation of a novel nanoparticulate-based
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Jeon O et al (2008) lsquoLong-term delivery enhances in vivo osteogenic efficacy of bone
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Ji Y et al (2010) lsquoBMP-2PLGA delayed-release microspheres composite graft selection of
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formation for maxillary sinus augmentationrsquo The International journal of periodontics amp
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Katranji A Fotek P and Wang H-L (2008) lsquoSinus augmentation complications etiology
and treatmentrsquo Implant dentistry 17(3) pp 339ndash349 doi
101097ID0b013e3181815660
Kempen D H R et al (2008) lsquoRetention of in vitro and in vivo BMP-2 bioactivities in
sustained delivery vehicles for bone tissue engineeringrsquo Biomaterials 29(22) pp 3245ndash
3252 doi 101016jbiomaterials200804031
Kempen D H R et al (2009) lsquoEffect of local sequential VEGF and BMP-2 delivery on ectopic
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Ki-Bum Lee Ani Solanki J Dongun Kim J J (2009) Nanomedicine Dynamic Integration of
Nanotechnology with Biomedical Science | Request PDF Available at
httpswwwresearchgatenetpublication254745458_Nanomedicine_Dynamic_Integrati
on_of_Nanotechnology_with_Biomedical_Science (Accessed 29 March 2020)
Kim H K W Oxendine I and Kamiya N (2013) lsquoHigh-concentration of BMP2 reduces cell
proliferation and increases apoptosis via DKK1 and SOST in human primary periosteal
cellsrsquo Bone 54(1) pp 141ndash150 doi 101016jbone201301031
Kim Y-H and Tabata Y (2015) lsquoDual-controlled release system of drugs for bone
regenerationrsquo Advanced drug delivery reviews 94 pp 28ndash40 doi
101016jaddr201506003
Kirby G T S et al (2011) lsquoPLGA-based microparticles for the sustained release of BMP-2rsquo
138
in European Cells and Materials AO Research Institute Davos p 24 doi
103390polym3010571
Kocbek P et al (2007) lsquoTargeting cancer cells using PLGA nanoparticles surface modified
with monoclonal antibodyrsquo Journal of controlled release official journal of the Controlled
Release Society 120(1ndash2) pp 18ndash26 doi 101016jjconrel200703012
Kok R J et al (1998) lsquoDrug delivery to the kidneys and the bladder with the low molecular
weight protein lysozymersquo Renal failure 20(2) pp 211ndash217 doi
10310908860229809045104
Kumar B et al (2017) lsquoRecent advances in nanoparticle-mediated drug deliveryrsquo Journal of
Drug Delivery Science and Technology Editions de Sante pp 260ndash268 doi
101016jjddst201707019
Kumar T R S Soppimath K and Nachaegari S K (2006) lsquoNovel delivery technologies for
protein and peptide therapeuticsrsquo Current pharmaceutical biotechnology 7(4) pp 261ndash
276 doi 102174138920106777950852
Kumari A Yadav S K and Yadav S C (2010) lsquoBiodegradable polymeric nanoparticles
based drug delivery systemsrsquo Colloids and surfaces B Biointerfaces 75(1) pp 1ndash18 doi
101016jcolsurfb200909001
La W-G et al (2010) lsquoThe efficacy of bone morphogenetic protein-2 depends on its mode
of deliveryrsquo Artificial organs 34(12) pp 1150ndash1153 doi 101111j1525-
1594200900988x
Lee J et al (2013) lsquoSinus augmentation using rhBMP-2ACS in a mini-pig model relative
efficacy of autogenous fresh particulate iliac bone graftsrsquo Clinical oral implants research
24(5) pp 497ndash504 doi 101111j1600-0501201102419x
Lee S-J et al (2017) lsquoDevelopment of Novel 3-D Printed Scaffolds With Core-Shell
Nanoparticles for Nerve Regenerationrsquo IEEE transactions on bio-medical engineering 64(2)
139
pp 408ndash418 doi 101109TBME20162558493
Li B et al (2009) lsquoThe effects of rhBMP-2 released from biodegradable
polyurethanemicrosphere composite scaffolds on new bone formation in rat femorarsquo
Biomaterials 30(35) pp 6768ndash6779 doi 101016jbiomaterials200908038
Liang C-C Park A Y and Guan J-L (2007) lsquoIn vitro scratch assay a convenient and
inexpensive method for analysis of cell migration in vitrorsquo Nature protocols 2(2) pp 329ndash
333 doi 101038nprot200730
LIN Y et al (2007) lsquoIn vitro Evaluation of Lysozyme-loaded Microspheres in
Thermosensitive Methylcellulose-based Hydrogel1 1 Supported by the National Natural
Science Foundation of China (No20576057) and Fundamental Research Foundation of
Tsinghua University (JCqn2005033)rsquo Chinese Journal of Chemical Engineering 15(4) pp
566ndash572 doi 101016S1004-9541(07)60125-6
Lochmann A et al (2010) lsquoThe influence of covalently linked and free polyethylene glycol
on the structural and release properties of rhBMP-2 loaded microspheresrsquo Journal of
controlled release official journal of the Controlled Release Society 147(1) pp 92ndash100 doi
101016jjconrel201006021
Loureiro J A et al (2016) lsquoCellular uptake of PLGA nanoparticles targeted with anti-amyloid
and anti-transferrin receptor antibodies for Alzheimerrsquos disease treatmentrsquo Colloids and
surfaces B Biointerfaces 145 pp 8ndash13 doi 101016jcolsurfb201604041
Luginbuehl V et al (2004) lsquoLocalized delivery of growth factors for bone repairrsquo European
journal of pharmaceutics and biopharmaceutics official journal of Arbeitsgemeinschaft fur
Pharmazeutische Verfahrenstechnik eV 58(2) pp 197ndash208 doi
101016jejpb200403004
M Padial-Molina P Galindo-Moreno G Aacute-M (2009) lsquoBiomimetic ceramics in implant
dentistryrsquo MINERVA BIOTECNOLOGICA 21 p 173
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Makadia H K and Siegel S J (2011) lsquoPoly Lactic-co-Glycolic Acid (PLGA) as Biodegradable
Controlled Drug Delivery Carrierrsquo Polymers 3(3) pp 1377ndash1397 doi
103390polym3031377
Maldonado-Valderrama J et al (2013) lsquoIn vitro digestion of interfacial protein structuresrsquo
Soft Matter 9(4) pp 1043ndash1053 doi 101039c2sm26843d
Mason S et al (2014) lsquoStandardization and safety of alveolar bone-derived stem cell
isolationrsquo Journal of dental research 93(1) pp 55ndash61 doi 1011770022034513510530
McClements D J (2018) lsquoEncapsulation protection and delivery of bioactive proteins and
peptides using nanoparticle and microparticle systems A reviewrsquo Advances in colloid and
interface science 253 pp 1ndash22 doi 101016jcis201802002
McKay W F Peckham S M and Badura J M (2007) lsquoA comprehensive clinical review of
recombinant human bone morphogenetic protein-2 (INFUSE Bone Graft)rsquo International
orthopaedics 31(6) pp 729ndash734 doi 101007s00264-007-0418-6
Meinel L et al (2001) lsquoStabilizing insulin-like growth factor-I in poly(DL-lactide-co-
glycolide) microspheresrsquo Journal of controlled release official journal of the Controlled
Release Society 70(1ndash2) pp 193ndash202 doi 101016s0168-3659(00)00352-7
Meng F T et al (2003) lsquoWOW double emulsion technique using ethyl acetate as organic
solvent effects of its diffusion rate on the characteristics of microparticlesrsquo Journal of
controlled release official journal of the Controlled Release Society 91(3) pp 407ndash416 doi
101016s0168-3659(03)00273-6
Mir M Ahmed N and Rehman A U (2017) lsquoRecent applications of PLGA based
nanostructures in drug deliveryrsquo Colloids and surfaces B Biointerfaces 159 pp 217ndash231
doi 101016jcolsurfb201707038
Misch C E (1987) lsquoMaxillary sinus augmentation for endosteal implants organized
alternative treatment plansrsquo The International journal of oral implantology implantologist
141
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Mohamed F and van der Walle C F (2008) lsquoEngineering biodegradable polyester particles
with specific drug targeting and drug release propertiesrsquo Journal of pharmaceutical sciences
97(1) pp 71ndash87 doi 101002jps21082
Morille M et al (2013) lsquoNew PLGA-P188-PLGA matrix enhances TGF-beta3 release from
pharmacologically active microcarriers and promotes chondrogenesis of mesenchymal
stem cellsrsquo Journal of controlled release official journal of the Controlled Release Society
170(1) pp 99ndash110 doi 101016jjconrel201304017
Mueller T D and Nickel J (2012) lsquoPromiscuity and specificity in BMP receptor activationrsquo
FEBS letters 586(14) pp 1846ndash1859 doi 101016jfebslet201202043
Myeroff C and Archdeacon M (2011) lsquoAutogenous bone graft donor sites and techniquesrsquo
The Journal of bone and joint surgery American volume 93(23) pp 2227ndash2236 doi
102106JBJSJ01513
Nair B P and Sharma C P (2012) lsquoPoly(lactide-co-glycolide)-laponite-F68 nanocomposite
vesicles through a single-step double-emulsion method for the controlled release of
doxorubicinrsquo Langmuir the ACS journal of surfaces and colloids 28(9) pp 4559ndash4564 doi
101021la300005c
Nevins M et al (1996) lsquoBone formation in the goat maxillary sinus induced by absorbable
collagen sponge implants impregnated with recombinant human bone morphogenetic
protein-2rsquo The International journal of periodontics amp restorative dentistry 16(1) pp 8ndash19
Oh S H Kim T H and Lee J H (2011) lsquoCreating growth factor gradients in three
dimensional porous matrix by centrifugation and surface immobilizationrsquo Biomaterials
32(32) pp 8254ndash8260 doi 101016jbiomaterials201107027
Ortega-Oller I et al (2015) lsquoBone Regeneration from PLGA Micro-Nanoparticlesrsquo BioMed
research international 2015 p 415289 doi 1011552015415289
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Ortega-Oller I et al (2017) lsquoDual delivery nanosystem for biomolecules Formulation
characterization and in vitro releasersquo Colloids and surfaces B Biointerfaces 159 pp 586ndash
595 doi 101016jcolsurfb201708027
Padial-Molina M et al (2012) lsquoMethods to validate tooth-supporting regenerative
therapiesrsquo Methods in molecular biology (Clifton NJ) 887 pp 135ndash148 doi 101007978-
1-61779-860-3_13
Padial-Molina M OrsquoValle F et al (2015) lsquoClinical Application of Mesenchymal Stem Cells
and Novel Supportive Therapies for Oral Bone Regenerationrsquo BioMed research
international 2015 p 341327 doi 1011552015341327
Padial-Molina M Rodriguez J C et al (2015) lsquoStandardized in vivo model for studying
novel regenerative approaches for multitissue bone-ligament interfacesrsquo Nature protocols
10(7) pp 1038ndash1049 doi 101038nprot2015063
Padial-Molina M et al (2019) lsquoExpression of Musashi-1 During Osteogenic Differentiation
of Oral MSC An In Vitro Studyrsquo International journal of molecular sciences 20(9) doi
103390ijms20092171
Padial-Molina M and Rios H F (2014) lsquoStem Cells Scaffolds and Gene Therapy for
Periodontal Engineeringrsquo Current Oral Health Reports 1(1) pp 16ndash25 doi
101007s40496-013-0002-7
Padial-Molina M Volk S L and Rios H F (2014) lsquoPeriostin increases migration and
proliferation of human periodontal ligament fibroblasts challenged by tumor necrosis factor
-alpha and Porphyromonas gingivalis lipopolysaccharidesrsquo Journal of periodontal research
49(3) pp 405ndash414 doi 101111jre12120
Paillard-Giteau A et al (2010) lsquoEffect of various additives and polymers on lysozyme
release from PLGA microspheres prepared by an sow emulsion techniquersquo European
journal of pharmaceutics and biopharmaceutics official journal of Arbeitsgemeinschaft fur
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Pharmazeutische Verfahrenstechnik eV 75(2) pp 128ndash136 doi
101016jejpb201003005
Pakulska M M et al (2016) lsquoEncapsulation-free controlled release Electrostatic adsorption
eliminates the need for protein encapsulation in PLGA nanoparticlesrsquo Science advances
2(5) p e1600519 doi 101126sciadv1600519
Pantazis P et al (2012) lsquoPreparation of siRNA-encapsulated PLGA nanoparticles for
sustained release of siRNA and evaluation of encapsulation efficiencyrsquo Methods in molecular
biology (Clifton NJ) 906 pp 311ndash319 doi 101007978-1-61779-953-2_25
Panyam J and Labhasetwar V (2003) lsquoDynamics of endocytosis and exocytosis of poly(DL-
lactide-co-glycolide) nanoparticles in vascular smooth muscle cellsrsquo Pharmaceutical
research 20(2) pp 212ndash220 doi 101023a1022219003551
Paolicelli P et al (2010) lsquoSurface-modified PLGA-based nanoparticles that can efficiently
associate and deliver virus-like particlesrsquo Nanomedicine (London England) 5(6) pp 843ndash
853 doi 102217nnm1069
Park J S et al (2013) lsquoMultilineage differentiation of human-derived dermal fibroblasts
transfected with genes coated on PLGA nanoparticles plus growth factorsrsquo Biomaterials
34(2) pp 582ndash597 doi 101016jbiomaterials201210001
Penaloza J P et al (2017) lsquoIntracellular trafficking and cellular uptake mechanism of PHBV
nanoparticles for targeted delivery in epithelial cell linesrsquo Journal of nanobiotechnology
15(1) p 1 doi 101186s12951-016-0241-6
Perez C De Jesus P and Griebenow K (2002) lsquoPreservation of lysozyme structure and
function upon encapsulation and release from poly(lactic-co-glycolic) acid microspheres
prepared by the water-in-oil-in-water methodrsquo International journal of pharmaceutics
248(1ndash2) pp 193ndash206 doi 101016s0378-5173(02)00435-0
Peula-Garcia J M Hidaldo-Alvarez R and De las Nieves F J (1997) lsquoProtein co-adsorption
144
on different polystyrene latexes Electrokinetic characterization and colloidal stabilityrsquo
Colloid and Polymer Science 275(2) pp 198ndash202 doi 101007s003960050072
Peula-Garciacutea J M Hidalgo-Alvarez R and De Las Nieves F J (1997) lsquoColloid stability and
electrokinetic characterization of polymer colloids prepared by different methodsrsquo Colloids
and Surfaces A Physicochemical and Engineering Aspects 127(1ndash3) pp 19ndash24 doi
101016S0927-7757(96)03890-3
Peula JM Callejas J de las Nieves F J (1994) lsquoAdsorption of Monomeric Bovine Serum
Albumin on Sulfonated Polystyrene Model Colloids II Electrokinetic Characterization of
Latex-Protein Complexesrsquo Surface Properties of Biomaterials pp 61ndash69
Peula J M Hidalgo-Alvarez R and de las Nieves F J (1995) lsquoCoadsorption of IgG and BSA
onto sulfonated polystyrene latex I Sequential and competitive coadsorption isothermsrsquo
Journal of biomaterials science Polymer edition 7(3) pp 231ndash240 doi
101163156856295x00274
Peula J M and de las Nieves F J (1993) lsquoAdsorption of monomeric bovine serum albumin
on sulfonated polystyrene model colloids 1 Adsorption isotherms and effect of the surface
charge densityrsquo Colloids and Surfaces A Physicochemical and Engineering Aspects 77(3) pp
199ndash208 doi 1010160927-7757(93)80117-W
Peula J M and de las Nieves F J (1994) lsquoAdsorption of monomeric bovine serum albumin
on sulfonated polystyrene model colloids 3 Colloidal stability of latex-protein complexesrsquo
Colloids and Surfaces A Physicochemical and Engineering Aspects 90(1) pp 55ndash62 doi
1010160927-7757(94)02889-3
Pezennec S et al (2008) The protein net electric charge determines the surface rheological
properties of ovalbumin adsorbed at the air-water interface Available at
wwwelseviercomlocatefoodhyd (Accessed 29 March 2020)
Pirooznia N et al (2012) lsquoEncapsulation of alpha-1 antitrypsin in PLGA nanoparticles in
145
vitro characterization as an effective aerosol formulation in pulmonary diseasesrsquo Journal of
nanobiotechnology 10 p 20 doi 1011861477-3155-10-20
Poinern G E J (no date) A laboratory course in nanoscience and nanotechnology
Puppi D et al (2014) lsquoNanomicrofibrous polymeric constructs loaded with bioactive
agents and designed for tissue engineering applications a reviewrsquo Journal of biomedical
materials research Part B Applied biomaterials 102(7) pp 1562ndash1579 doi
101002jbmb33144
Qutachi O Shakesheff K M and Buttery L D K (2013) lsquoDelivery of definable number of
drug or growth factor loaded poly(DL-lactic acid-co-glycolic acid) microparticles within
human embryonic stem cell derived aggregatesrsquo Journal of controlled release official
journal of the Controlled Release Society 168(1) pp 18ndash27 doi
101016jjconrel201302029
Rafati A et al (2012) lsquoChemical and spatial analysis of protein loaded PLGA microspheres
for drug delivery applicationsrsquo Journal of controlled release official journal of the Controlled
Release Society 162(2) pp 321ndash329 doi 101016jjconrel201205008
Rahman C V et al (2014) lsquoControlled release of BMP-2 from a sintered polymer scaffold
enhances bone repair in a mouse calvarial defect modelrsquo Journal of tissue engineering and
regenerative medicine 8(1) pp 59ndash66 doi 101002term1497
Ramel M-C and Hill C S (2012) lsquoSpatial regulation of BMP activityrsquo FEBS letters 586(14)
pp 1929ndash1941 doi 101016jfebslet201202035
Ratzinger G et al (2010) lsquoSurface modification of PLGA particles the interplay between
stabilizer ligand size and hydrophobic interactionsrsquo Langmuir the ACS journal of surfaces
and colloids 26(3) pp 1855ndash1859 doi 101021la902602z
Rescignano N et al (2016) lsquoIn-vitro degradation of PLGA nanoparticles in aqueous medium
and in stem cell cultures by monitoring the cargo fluorescence spectrumrsquo Polymer
146
Degradation and Stability 134 pp 296ndash304 doi 101016jpolymdegradstab201610017
van Rijt S and Habibovic P (2017) lsquoEnhancing regenerative approaches with
nanoparticlesrsquo Journal of the Royal Society Interface 14(129) doi 101098rsif20170093
Romagnoli C DrsquoAsta F and Brandi M L (2013) lsquoDrug delivery using composite scaffolds
in the context of bone tissue engineeringrsquo Clinical cases in mineral and bone metabolism
the official journal of the Italian Society of Osteoporosis Mineral Metabolism and Skeletal
Diseases 10(3) pp 155ndash161
Ronga M et al (2013) lsquoClinical applications of growth factors in bone injuries experience
with BMPsrsquo Injury 44 Suppl 1 pp S34-9 doi 101016S0020-1383(13)70008-1
Rosca I D Watari F and Uo M (2004) lsquoMicroparticle formation and its mechanism in
single and double emulsion solvent evaporationrsquo Journal of controlled release official
journal of the Controlled Release Society 99(2) pp 271ndash280 doi
101016jjconrel200407007
Sanchez-Moreno P et al (2013) lsquoSynthesis and characterization of lipid immuno-
nanocapsules for directed drug delivery selective antitumor activity against HER2 positive
breast-cancer cellsrsquo Biomacromolecules 14(12) pp 4248ndash4259 doi 101021bm401103t
Santander-Ortega M J et al (2006) lsquoColloidal stability of pluronic F68-coated PLGA
nanoparticles a variety of stabilisation mechanismsrsquo Journal of colloid and interface science
302(2) pp 522ndash529 doi 101016jjcis200607031
Santander-Ortega M J et al (2009) lsquoInsulin-loaded PLGA nanoparticles for oral
administration an in vitro physico-chemical characterizationrsquo Journal of biomedical
nanotechnology 5(1) pp 45ndash53 doi 101166jbn2009022
Santander-Ortega M J et al (2010) lsquoNanoparticles made from novel starch derivatives for
transdermal drug deliveryrsquo Journal of controlled release official journal of the Controlled
Release Society 141(1) pp 85ndash92 doi 101016jjconrel200908012
147
Santander-Ortega Manuel J Lozano-Loacutepez M V et al (2010) lsquoNovel core-shell lipid-
chitosan and lipid-poloxamer nanocapsules Stability by hydration forcesrsquo Colloid and
Polymer Science 288(2) pp 159ndash172 doi 101007s00396-009-2132-y
Santander-Ortega Manuel J Csaba N et al (2010) lsquoProtein-loaded PLGA-PEO blend
nanoparticles Encapsulation release and degradation characteristicsrsquo Colloid and Polymer
Science 288(2) pp 141ndash150 doi 101007s00396-009-2131-z
Santander-Ortega M J et al (2011) lsquoChitosan nanocapsules Effect of chitosan molecular
weight and acetylation degree on electrokinetic behaviour and colloidal stabilityrsquo Colloids
and surfaces B Biointerfaces 82(2) pp 571ndash580 doi 101016jcolsurfb201010019
Santander-Ortega M J Bastos-Gonzalez D and Ortega-Vinuesa J L (2007)
lsquoElectrophoretic mobility and colloidal stability of PLGA particles coated with IgGrsquo Colloids
and surfaces B Biointerfaces 60(1) pp 80ndash88 doi 101016jcolsurfb200706002
Santo V E et al (2012) lsquoFrom nano- to macro-scale nanotechnology approaches for
spatially controlled delivery of bioactive factors for bone and cartilage engineeringrsquo
Nanomedicine (London England) 7(7) pp 1045ndash1066 doi 102217nnm1278
Sapkota G et al (2007) lsquoBalancing BMP signaling through integrated inputs into the Smad1
linkerrsquo Molecular cell 25(3) pp 441ndash454 doi 101016jmolcel200701006
Schrier J A et al (2001) lsquoEffect of a freeze-dried CMCPLGA microsphere matrix of rhBMP-
2 on bone healingrsquo AAPS PharmSciTech 2(3) p E18 doi 101208pt020318
Schrier J A and DeLuca P P (2001) lsquoPorous bone morphogenetic protein-2 microspheres
polymer binding and in vitro releasersquo AAPS PharmSciTech 2(3) p E17 doi
101208pt020317
Schwendeman S P et al (2014) lsquoInjectable controlled release depots for large moleculesrsquo
Journal of controlled release official journal of the Controlled Release Society 190 pp 240ndash
253 doi 101016jjconrel201405057
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Shankarayan R Kumar S and Mishra P (2013) lsquoDifferential permeation of piroxicam-
loaded PLGA micronanoparticles and their in vitro enhancementrsquo Journal of Nanoparticle
Research 15(3) doi 101007s11051-013-1496-6
Shim Y B et al (2016) lsquoFabrication of hollow porous PLGA microspheres using sucrose for
controlled dual delivery of dexamethasone and BMP2rsquo Journal of Industrial and Engineering
Chemistry 37 pp 101ndash106 doi 101016jjiec201603014
Siafaka P I et al (2016) lsquoSurface Modified Multifunctional and Stimuli Responsive
Nanoparticles for Drug Targeting Current Status and Usesrsquo International journal of
molecular sciences 17(9) doi 103390ijms17091440
Sieber C et al (2009) lsquoRecent advances in BMP receptor signalingrsquo Cytokine amp growth factor
reviews 20(5ndash6) pp 343ndash355 doi 101016jcytogfr200910007
Silva G A et al (2007) lsquoMaterials in particulate form for tissue engineering 2 Applications
in bonersquo Journal of tissue engineering and regenerative medicine 1(2) pp 97ndash109 doi
101002term1
Simmonds M C et al (2013) lsquoSafety and effectiveness of recombinant human bone
morphogenetic protein-2 for spinal fusion a meta-analysis of individual-participant datarsquo
Annals of internal medicine 158(12) pp 877ndash889 doi 1073260003-4819-158-12-
201306180-00005
Sneh-Edri H Likhtenshtein D and Stepensky D (2011) lsquoIntracellular targeting of PLGA
nanoparticles encapsulating antigenic peptide to the endoplasmic reticulum of dendritic
cells and its effect on antigen cross-presentation in vitrorsquo Molecular pharmaceutics 8(4)
pp 1266ndash1275 doi 101021mp200198c
Spagnoli D B and Marx R E (2011) lsquoDental implants and the use of rhBMP-2rsquo Dental clinics
of North America 55(4) pp 883ndash907 doi 101016jcden201107014
Srinivasan C et al (2005) lsquoEffect of additives on encapsulation efficiency stability and
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bioactivity of entrapped lysozyme from biodegradable polymer particlesrsquo Journal of
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Sturesson C and Carlfors J (2000) lsquoIncorporation of protein in PLG-microspheres with
retention of bioactivityrsquo Journal of controlled release official journal of the Controlled
Release Society 67(2ndash3) pp 171ndash178 doi 101016s0168-3659(00)00205-4
Sun D (2016) lsquoEffect of Zeta Potential and Particle Size on the Stability of SiO2 Nanospheres
as Carrier for Ultrasound Imaging Contrast Agentsrsquo Int J Electrochem Sci pp 8520ndash8529
Tan J S et al (1993) lsquoSurface modification of nanoparticles by PEOPPO block copolymers
to minimize interactions with blood components and prolong blood circulation in ratsrsquo
Biomaterials 14(11) pp 823ndash833 doi 1010160142-9612(93)90004-l
Tian Z et al (2012) lsquoSynthesis and characterization of UPPE-PLGA-rhBMP2 scaffolds for
bone regenerationrsquo Journal of Huazhong University of Science and Technology Medical
sciences = Hua zhong ke ji da xue xue bao Yi xue Ying De wen ban = Huazhong keji daxue
xuebao Yixue Yingdewen ban 32(4) pp 563ndash570 doi 101007s11596-012-0097-4
Torcello-Goacutemez A et al (2011) lsquoAdsorption of antibody onto Pluronic F68-covered
nanoparticles Link with surface propertiesrsquo Soft Matter 7(18) pp 8450ndash8461 doi
101039c1sm05570d
Torrecillas-Martinez L et al (2013) lsquoEffect of rhBMP-2 upon maxillary sinus augmentation
a comprehensive reviewrsquo Implant dentistry 22(3) pp 232ndash237 doi
101097ID0b013e31829262a8
Tran M-K Swed A and Boury F (2012) lsquoPreparation of polymeric particles in CO(2)
medium using non-toxic solvents formulation and comparisons with a phase separation
methodrsquo European journal of pharmaceutics and biopharmaceutics official journal of
Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik eV 82(3) pp 498ndash507 doi
101016jejpb201208005
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Tsuji K et al (2006) lsquoBMP2 activity although dispensable for bone formation is required
for the initiation of fracture healingrsquo Nature genetics 38(12) pp 1424ndash1429 doi
101038ng1916
Tsuji K et al (2008) lsquoBMP4 is dispensable for skeletogenesis and fracture-healing in the
limbrsquo The Journal of bone and joint surgery American volume 90 Suppl 1 pp 14ndash18 doi
102106JBJSG01109
Tsuji K et al (2010) lsquoConditional deletion of BMP7 from the limb skeleton does not affect
bone formation or fracture repairrsquo Journal of orthopaedic research official publication of
the Orthopaedic Research Society 28(3) pp 384ndash389 doi 101002jor20996
Urist M R (1965) lsquoBone formation by autoinductionrsquo Science (New York NY) 150(3698)
pp 893ndash899 doi 101126science1503698893
Vasir J K and Labhasetwar V (2007) lsquoBiodegradable nanoparticles for cytosolic delivery
of therapeuticsrsquo Advanced drug delivery reviews 59(8) pp 718ndash728 doi
101016jaddr200706003
Vo T N Kasper F K and Mikos A G (2012) lsquoStrategies for controlled delivery of growth
factors and cells for bone regenerationrsquo Advanced drug delivery reviews 64(12) pp 1292ndash
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Wallace S S and Froum S J (2003) lsquoEffect of maxillary sinus augmentation on the survival
of endosseous dental implants A systematic reviewrsquo Annals of periodontology 8(1) pp
328ndash343 doi 101902annals200381328
Wan F and Yang M (2016) lsquoDesign of PLGA-based depot delivery systems for
biopharmaceuticals prepared by spray dryingrsquo International journal of pharmaceutics
498(1ndash2) pp 82ndash95 doi 101016jijpharm201512025
Wang E A et al (1990) lsquoRecombinant human bone morphogenetic protein induces bone
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Wang H et al (2012) lsquoThe use of micro- and nanospheres as functional components for
bone tissue regenerationrsquo Tissue engineering Part B Reviews 18(1) pp 24ndash39 doi
101089tenTEB20110184
Wang Y et al (2015) lsquoPLGAPDLLA core-shell submicron spheres sequential release
system Preparation characterization and promotion of bone regeneration in vitro and in
vivorsquo Chemical Engineering Journal 273 pp 490ndash501 doi 101016jcej201503068
Wege H A Holgado-Terriza J A and Cabrerizo-Vilchez M A (2002) lsquoDevelopment of a
constant surface pressure penetration langmuir balance based on axisymmetric drop shape
analysisrsquo Journal of colloid and interface science 249(2) pp 263ndash273 doi
101006jcis20028233
Wheeler S L (1997) lsquoSinus augmentation for dental implants the use of alloplastic
materialsrsquo Journal of oral and maxillofacial surgery official journal of the American
Association of Oral and Maxillofacial Surgeons 55(11) pp 1287ndash1293 doi 101016s0278-
2391(97)90186-5
White L J et al (2013) lsquoAccelerating protein release from microparticles for regenerative
medicine applicationsrsquo Materials science amp engineering C Materials for biological
applications 33(5) pp 2578ndash2583 doi 101016jmsec201302020
Wozney J M (1992) lsquoThe bone morphogenetic protein family and osteogenesisrsquo Molecular
reproduction and development 32(2) pp 160ndash167 doi 101002mrd1080320212
Xia Y et al (2013) lsquoProtein encapsulation in and release from monodisperse double-wall
polymer microspheresrsquo Journal of pharmaceutical sciences 102(5) pp 1601ndash1609 doi
101002jps23511
Xiong S et al (2011) lsquoCellular uptake of Poly-(DL-lactide-co-glycolide) (PLGA)
nanoparticles synthesized through solvent emulsion evaporation and nanoprecipitation
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methodrsquo Biotechnology journal 6(5) pp 501ndash508 doi 101002biot201000351
Xu Y et al (2017) lsquoPolymer degradation and drug delivery in PLGA-based drug-polymer
applications A review of experiments and theoriesrsquo Journal of biomedical materials
research Part B Applied biomaterials 105(6) pp 1692ndash1716 doi 101002jbmb33648
Yallapu M M et al (2010) lsquoFabrication of curcumin encapsulated PLGA nanoparticles for
improved therapeutic effects in metastatic cancer cellsrsquo Journal of colloid and interface
science 351(1) pp 19ndash29 doi 101016jjcis201005022
Yameen B et al (2014) lsquoInsight into nanoparticle cellular uptake and intracellular
targetingrsquo Journal of controlled release official journal of the Controlled Release Society 190
pp 485ndash499 doi 101016jjconrel201406038
Yang Y Y Chia H H and Chung T S (2000) lsquoEffect of preparation temperature on the
characteristics and release profiles of PLGA microspheres containing protein fabricated by
double-emulsion solvent extractionevaporation methodrsquo Journal of controlled release
official journal of the Controlled Release Society 69(1) pp 81ndash96 doi 101016s0168-
3659(00)00291-1
Yang Y Y Chung T S and Ng N P (2001) lsquoMorphology drug distribution and in vitro
release profiles of biodegradable polymeric microspheres containing protein fabricated by
double-emulsion solvent extractionevaporation methodrsquo Biomaterials 22(3) pp 231ndash
241 doi 101016s0142-9612(00)00178-2
Yilgor P et al (2009) lsquoIncorporation of a sequential BMP-2BMP-7 delivery system into
chitosan-based scaffolds for bone tissue engineeringrsquo Biomaterials 30(21) pp 3551ndash3559
doi 101016jbiomaterials200903024
Yilgor P Hasirci N and Hasirci V (2010) lsquoSequential BMP-2BMP-7 delivery from
polyester nanocapsulesrsquo Journal of biomedical materials research Part A 93(2) pp 528ndash
536 doi 101002jbma32520
153
Zhang H-X et al (2016) lsquoIn vitro and in vivo evaluation of calcium phosphate composite
scaffolds containing BMP-VEGF loaded PLGA microspheres for the treatment of avascular
necrosis of the femoral headrsquo Materials science amp engineering C Materials for biological
applications 60 pp 298ndash307 doi 101016jmsec201511055
Zhang S and Uludag H (2009) lsquoNanoparticulate systems for growth factor deliveryrsquo
Pharmaceutical research 26(7) pp 1561ndash1580 doi 101007s11095-009-9897-z
154
10ANEXO MATERIAL SUPLEMENTARIO
httprsbwebnihgovij
155
156
11ANEXO DE PUBLICACIONES
Review ArticleBone Regeneration from PLGA Micro-Nanoparticles
Inmaculada Ortega-Oller1 Miguel Padial-Molina1 Pablo Galindo-Moreno1
Francisco OrsquoValle2 Ana Beleacuten Joacutedar-Reyes3 and Jose Manuel Peula-Garciacutea34
1Department of Oral Surgery and Implant Dentistry University of Granada 18011 Granada Spain2Department of Pathology School of Medicine and IBIMER University of Granada 18012 Granada Spain3Biocolloid and Fluid Physics Group Department of Applied Physics University of Granada 18071 Granada Spain4Department of Applied Physics II University of Malaga 29071 Malaga Spain
Correspondence should be addressed to Jose Manuel Peula-Garcıa jmpeulaumaes
Received 27 March 2015 Accepted 4 June 2015
Academic Editor Hojae Bae
Copyright copy 2015 Inmaculada Ortega-Oller et al This is an open access article distributed under the Creative CommonsAttribution License which permits unrestricted use distribution and reproduction in any medium provided the original work isproperly cited
Poly-lactic-co-glycolic acid (PLGA) is one of the most widely used synthetic polymers for development of delivery systems fordrugs and therapeutic biomolecules and as component of tissue engineering applications Its properties and versatility allow it tobe a reference polymer in manufacturing of nano- and microparticles to encapsulate and deliver a wide variety of hydrophobic andhydrophilic molecules It additionally facilitates and extends its use to encapsulate biomolecules such as proteins or nucleic acidsthat can be released in a controlled wayThis review focuses on the use of nanomicroparticles of PLGA as a delivery system of oneof the most commonly used growth factors in bone tissue engineering the bone morphogenetic protein 2 (BMP2) Thus all theneeded requirements to reach a controlled delivery of BMP2 using PLGA particles as a main component have been examinedTheproblems and solutions for the adequate development of this system with a great potential in cell differentiation and proliferationprocesses under a bone regenerative point of view are discussed
1 Introduction
Bone regeneration is one of the main challenges facing us inthe daily clinic Immediately after a tooth extraction normalbiological processes remodel the alveolar bone limiting insome cases the possibility of future implant placementDifferent strategies for the preservation of that bone havebeen explored in recent years Other conditions such astrauma tumor resective surgery or congenital deformitiesrequire even higher technical and biological requirementsto generate the necessary bony structure for the occlusalrehabilitation of the patient To overcome these anatomicallimitations in terms of bone volume different approacheshave been proposed to either improve the implant osteoin-tegration or to augment the bone anatomy where it will beplaced [1 2] Autogenous bone graft is still considered theldquogold standardrdquo due to its osteogenic osteoconductive andosteoconductive properties [3 4] However it also presentsseveral limitations including the need for a second surgery
limited availability and morbidity in the donor area [5]Therefore other biomaterials such as allogeneic grafts withosteoconductivity and osteoinductive capacities [6 7] andxenogeneic grafts [8 9] and alloplastic biomaterials [10]with osseoconductive potential were proposed All thesematerials although acceptable are not suitable in manyconditions and usually require additional consideration inthe decision process [11] Additionally the bone quantity andquality that can be obtained with these materials are oftenlimited
The use of bioactive molecules alone or in combina-tion with the previously described materials has thereforebecome amajor area of interest thanks to their high potentialWhen using this kind of procedures it is important toconsider (1) the delivery method and (2) the molecule itselfBioactive molecules can be transported into the defect areaas a solution or a gel embedded in sponges adhered to solidscaffolds and more recently included in particles of differentsizes Using these methods PDGF (platelet-derived growth
Hindawi Publishing CorporationBioMed Research InternationalVolume 2015 Article ID 415289 18 pageshttpdxdoiorg1011552015415289
2 BioMed Research International
factor) FGF (fibroblast growth factor) IGF (insulin growthfactor) Runx2 Osterix (Osx) LIM domain mineralizationprotein (LMP) BMP (bone morphogenic protein) and morerecently periostin have been proposed as potential candidatesfor regeneration procedures within the oral cavity includingbone and periodontal tissues [12 13] These molecules havebeen tested alone or in combination with stem cells [14] usingseveral in vitro and in vivo strategies [15]
Consequently within the context of this review we intendto review the delivery methods of bioactive molecules withthe purpose of bone regeneration with a particular focus onpolymeric nanomicroparticles especially those with PLGAas main component to encapsulate the growth factor BMP-2 An overview of the biological functions of bone morpho-genetic proteins and an analysis of the different parametersaffecting the physicochemical properties of these systems arepresented Synthesis method particle size and morphologyuse of stabilizers and their incidence in the colloidal sta-bility protective function and surface functionality will bediscussed In addition we explore the different strategies thatcan be used to optimize the encapsulation efficiency andrelease kinetics main parameters that determine the correctdevelopment of polymeric carriers used in tissue-engineeredbone processes
2 BMPs Action and Regulation
For bone regeneration in particular bone morphogeneticgrowth factors (BMP) are probably the more tested groupof molecules Since 1965 when Urist [16] showed that theextracted bone BMPs could induce bone and cartilage forma-tion when implanted in animal tissue an increasing numberof reports have tested its in vivo application and biologicalfoundation when used in bone defects [17ndash19] BMPs aremembers of the TGF-120573 superfamily of proteins [20] TheBMP family of proteins groups more than 20 homodimericor heterodimericmorphogenetic proteins which functions inmany cell types and tissues not all of them being osteogenic[21] BMPs can be divided into 4 subfamilies based on theirfunction and sequence being BMP-2 BMP-4 and BMP-7 the ones with osteogenic potential [21] The actions ofBMPs include chondrogenesis osteogenesis angiogenesisand extracellular matrix synthesis [22] Within this fam-ily of proteins BMP-2 has been the most studied It hasosteoinductive properties that promote the formation of newbone by initiating stimulating and amplifying the cascadeof bone formation through chemotaxis and stimulationof proliferation and differentiation of the osteoblastic celllineage [5 17 19 20]The absence of it as studied in knockoutmodels leads to spontaneous fractures that do not heal withtime [23] In fact other models have demonstrated that theabsence of either BMP-4 [24] or BMP-7 [25] do not lead tobone formation and function impairmentwhich demonstratethe compensatory effect produced by BMP-2 alone [26]
Many cell types in bone tissue produce BMPs includingosteoprogenitor cells osteoblasts chrondrocytes plateletsand endothelial cells This secreted BMP is then stored in theextracellular matrix where it mostly interacts with collagentype IV [27] During the repair and remodeling processes
BMPs
BMPR-I BMPR-II
Smad 1 Smad 5
Smad 8Smad 4
Runx2 Dlx5 Osterix
Osteogenesis
Bone resorption
Figure 1 Schematic representation of the main BMP molecularpathway to osteogenesis BMPs interact with cell surface receptorsI and II to activate Smads 1 5 and 8 These activated Smads activateSmad 4 All together as a protein complex activate Runx2 Dlx5 andOsterix
osteoclast resorptive activity induces the release of BMPs tothe medium so that they are suspended and can interact withnearby cells to initiate the subsequent osteogenic process [28]
A BMP in the extracellular matrix binds to cell surfacereceptors BMPR-I and BMPR-II and activates the Smadcytoplasmic proteins or the MAPK pathway [29] WhenBMPR-I is activated BMPR-II is recruited and activatedas well [30] The activation of the complexes BMPR-I andBMPR-II leads to the activation of several Smads (1 5 and 8)that also activate Smad 4 and they all form protein complexesthat are transported into the nucleus where Runx2 Dlx5and Osterix genes (important in osteogenesis) are activated[26 27] (Figure 1) Similarly when the MAPK pathwayis activated it leads to induction of Runx2 transcriptionand therefore to bone differentiation [31] A number ofextracellular and intracellular antagonists have also beendescribed including noggin chordin and gremlin or Smads6 7 and 8b respectively [32]
21 Clinical Use of BMP-2 Today the BMP-2 is commerciallyavailable under different brand names and concentrations Itusually consists of a collagen absorbable sponge embeddedwith recombinant human BMP-2 In 2002 it was approvedby the FDA as an alternative of autogenous bone graftingin anterior lumbar interbody fusion [33] Later in 2007 theFDA approved the use of rhBMP-2 as an alternative forautogenous bone grafting in the increase of the alveolar crestdefects associated with the tooth extraction maxillary sinuspneumatization [33]
Beside the applications in spine clinical studies wherevery high concentrations are used (AMPLIFY rhBMP-240mg) clinical studies have supported its use in the oral
BioMed Research International 3
cavity BMPs have been used in periodontal regenerationbone healing implant osteointegration oral surgery withorthodontic purposes bone pathology sequel repair distrac-tion osteogenesis and endodontic reparative surgery [28 34]However it has shownmore promising results in cases whereonly bone tissue is to be regenerated including preimplantsite development sinus lift vertical and horizontal ridgeaugmentation and dental implant wound healing [35] In thissense it has been shown that the use of rhBMP-2 induced theformation of bone suitable for placement of dental implantsand their osteointegration [36] Furthermore it appears thatthe newly formed bone has similar properties to the nativebone and is therefore capable of supporting denture occlusalforces [37] In the particular case of sinus lifting where bonedeficiency is greater and therefore supportive therapies canbe more helpful a recent meta-analysis found a total of 3human studies and 4 animal trials (Table 1) [38] In summarythe included studies concluded that rhBMP-2 induces newbone formation with comparable bone quality and quantityof newly formed bone to that induced by autogenous bonegraft In some cases even higher bone quality and quantityhave been reported [39]
Conversely recent studies report severe complicationsafter its use [61] Even more high doses have also associatedwith carcinogenic effects which led the authors to emphasizethe need for better guidelines in BMP clinical use [62] Notso drastic recent studies are highlighting the negative sideeffects and risks of its application making high emphasison potential bias of nonreproducible industry sponsoredresearch especially when used in spinal fusion [44 63 64]The use of rhBMP-2 has been shown to increase the risks forwound complications and dysphagia with high effectivenessand harms misrepresentation through selective reportingduplicate publication and underreporting [44] Specificallyin oral bone regenerative applications a report in sinus liftconcluded that the use of BMP-2 promotes negative effectson bone formation when combined with anorganic bovinebone matrix versus anorganic bovine bone alone [41] incontrast with previous reports and reviews [38] Takingtogether this information it can be concluded that it is ofextreme importance to be careful with the clinical use of newproducts avoiding off-label applications It is also importantto highlight the need for more and better clinical research
To overcome these limitations new strategies such asthe use of ex vivo BMP-2-engineered autologous MSCs [65]encapsulation of the protein in different biomaterials ordelivery by gene therapy are being explored in recent years
The development of these technologies is based on somebiological facts In vitro effects of BMPs are observed at verylow dosages (5ndash20 ngmL) although current commerciallyavailable rhBMPs are used in large dosages (up to 40mg ofsome products) [28] This is probably due to an intense pro-teolytic consumption during the early postsurgical phases Itis important to know the proper sequence of biological eventsthat lead to normal tissue healing Then this knowledge canbe used to intervene at the specific time frame where ourtherapy is intended to act [15] Effective bone formation asdescribed above is a sequential processTherefore the induc-tive agent should be delivered at a maintained concentration
during a timeframe In this sense as in many other processesin medicine it has been recently demonstrated that long-term release of BMP-2 is more effective than short-term overa range of doses [51] It is also important to note that therole of other molecular pathways and crosstalk between thedifferent components playing in bone regeneration is notperfectly understood yet and therefore more research hasto be conducted
What is known so far in summary is that BMPs specif-ically BMP-2 is of utility for promoting bone regeneration[28] However the currently FDA-approved BMP-2 deliverysystem (INFUSE Medtronic Sofamor Danek Inc) presentsimportant limitations [66] Firstly protein is quickly inacti-vatedTherefore its biological action disappears maybe evenbefore the blood clot that forms after the surgery is beingorganized Second the recombinant protein is delivered inan absorbable collagen sponge Thus the distribution ofthe BMP in a liquid suspension embedded into a collagensponge makes it impossible to be certain that the protein isreaching the ideal target Therefore where when and forhow long a dose of BMP-2 is reached (determined by thedelivery method) are important factors Because of that newforms of BMP-2 delivery are being developed These newtechnologies have to guarantee a higher half-life of the proteinand a stepped release to increase the effects on the desiredcell targets The biotechnology opens the door to be able toprovide a solution to these limitations
Biodegradable nanoparticles (nanospheres and nanocap-sules) have developed as a promising important tool forthe delivery of macromolecules via parenteral mucous andtopical applications [67ndash70] Well-established biodegradablepolymers such as poly(acid D L-lactic) or poly(D L-lactic-co-glycolic) have been widely used in the preparation ofnanoparticles in recent decades because of its biocompati-bility and full biodegradability [71] However it is knownthat certain macromolecules such as proteins or peptidesmay lose activity during their encapsulation storage deliveryand release [72] To overcome this problem the addition ofstabilizers such as oxide polyethylene (PEO) or the coencap-sulation with other macromolecules and its derivatives seemto be a promising strategy
3 Polymeric Colloidal Particles to EncapsulateHydrophilic Molecules
Generally polymeric colloidal particles are hard systemswith a homogeneous spherical shape composed by naturalor synthetic polymers In order to encapsulate hydrophilicmolecules as proteins or nucleic acids it is necessary to opti-mize the polymeric composition and the synthesis methodIn this process a high encapsulation efficiency maintenanceof the biological activity of the encapsulated biomolecule andobtaining of an adequate release pattern have to be achieved[73ndash75] Several delivery systems of BMP2 (and other growthfactors GFs) using polymeric particles have been describedin the literature Most of them are microparticulated systemsusing the biocompatible and biodegradable PLGA copoly-mer as main component [76 77] Taking into account theincorporation of BMP2 to the carrier system encapsulation
4 BioMed Research InternationalTa
ble1
Sum
maryo
fclin
icalandanim
alstu
diesusingB
MP-2for
sinus
floor
elevatio
n(adapted
from[38])Th
eincludedstu
diesoverallcon
cludedthatrhBM
P-2ind
ucesnewbo
neform
ation
with
comparableb
oneq
ualityandqu
antityof
newlyform
edbo
neto
thatindu
cedby
autogeno
usbo
negraft
Reference
Stud
ydesig
nFo
llow-up
(mon
ths)
Species
(sub
jects)
Coreb
iopsy
harvestin
g(m
onths)
Graftmaterial
New
lyform
edbo
neBo
neheight
gain
(mm)
Bone
width
gain
(mm)
Bone
density
(mgmL)
Immun
erespon
seHistolog
y
Boyn
eetal
2005
[37]
RCT
52Hum
an(48)
6ndash11
075
mgmL
rhBM
P-2AC
S
NA
1129
Crest202
Midpo
int854
Apical118
684
Non
eNA
150m
gmL
rhBM
P-2AC
S047
Crest19
8Midpo
int78
0Ap
ical1078
134
Autogeno
usbo
negraft
autogenou
sbo
negraft
+allogeneicbo
negraft
1016
Crest466
Midpo
int
1017
Apical1056
350
Triplettetal
2009
[40]
RCT
58Hum
an(160)
6
150m
gmL
rhBM
P-2AC
S
NA
783plusmn352
NA
200
Non
e
Rich
vascular
marrowspaceh
ighin
cellu
larc
ontent
Autogeno
usbo
negraft
(iliacc
rest
tibia
ororalcavity)
autogeno
usbo
negraft
+allogeneic
bone
graft
946plusmn411
283
Oste
oclasts
stillpresenthigh
erfib
rous
tissue
Kaoetal2012
[41]
Prospective
6ndash9
Hum
an(22)
6ndash9
rhBM
P-2AC
S+
ABB
1604plusmn74
5
NA
NA
NA
Non
e
Fewer
ABB
particleslessnewlyform
edbo
ne(w
oven
andmatureb
ones
tructure)
ABB
2485plusmn582
MoreA
BBparticlesrem
aining
higher
newlyform
edbo
ne(w
oven
andmatured
bone
structure)
Nevinse
tal
1996
[36]
Prospective
12Goat(6)
12
rhBM
P-2AC
S
NA
NA
NA
NA
Non
e
Dense
isolatedtrabeculae
andbo
nemarrowoste
oblastandosteoclasts
no
corticalbo
ne
ACSBu
ffer
Collageno
usconn
ectiv
etissuen
oevidence
ofinflammation
noneo-osteogenesis
Hanisc
hetal
1997
[42]
RCT
24Non
human
prim
ate(12)
24rhBM
P-2AC
SNA
60plusmn03
NA
144plusmn29
NA
New
lyform
edbo
neindisting
uishable
from
resid
ualbon
eAC
S26plusmn03
139plusmn46
Wadae
tal
2001
[43]
Prospective
8Ra
bbit(10)
8rhBM
P-2AC
S224plusmn44
NA
NA
NA
NA
Cortic
albo
neform
ationin
both
grou
ps
trabeculae
with
clear
lamellarstructure
weree
mbedd
edin
fatty
marrow
Autogeno
usbo
negraft
(iliacc
rest)
219plusmn45
Leee
tal2013
[39]
Prospective
8Mini-p
ig(8)
8rhBM
P-2AC
SNA
93plusmn05
NA
519plusmn3
NA
New
lyform
edcancellous
bonenew
bone
continuo
uswith
resid
entb
onewoven
bone
infib
rovascular
andfatty
marrow
Autogeno
usbo
negraft
(iliacc
rest)
86plusmn07
329plusmn25
Irregu
lara
ndvaria
bleb
onea
mon
gdifferent
subjects
NAnot
availableRC
Trand
omized
clinicaltrialAC
Sabsorbablecollagenspon
geA
BBano
rganicbo
vine
bone
BioMed Research International 5
Hydrophilic biomolecules stabilizers
Organicphase
PLGA and stabilizers(surfactant other polymers) in
DCM acetone or EtAc
Mixture underagitationsonication
Ethanol water surfactants
Antibody
Core
PLGA BSABMP-2
Surface
Surfactant
MicronanosphereMicronanocapsule
ImmunoparticleDirected delivery
Organic solventextraction under
vacuumAqueous phase w1
First w1oemulsion
emulsion
Second polarphase w2
Final w1ow2
120
80
40
150mL
120
80
40
150mL
Figure 2 Double emulsion procedure (wateroilwater emulsion W1OW
2) to obtain PLGA micronanoparticles Depending on the
synthesis conditions (stabilizers solvents and mixing procedure) it is possible to obtain micro-nanospheres with a uniformmatrix or micro-nanocapsules with a core-shell structure Immunoparticles used for directed delivery can be obtained by attaching specific antibodymoleculeson the particle surface
is preferred to absorption because the growth factors aremore protected against environmental factors in the mediumand may have better control over the delivery and release toachieve the desired concentrations in specific site and time[78]
Normally if the GFs are related with bone regenerationprocesses nano-microparticles are trapped in a second sys-tem as hydrogels or tissue engineering scaffolds which alsoplay an important role in the release profile of GFs fromthese particles [78] The nano-microparticles have allowedthe development of multiscale scaffold thereby facilitatingcontrol of the internal architecture and adequate patterns ofmechanical gradients of cells and signaling factors [79]
All steps from the synthesis method and its characteris-tics the encapsulation process or the final surface modifica-tion for a targeted delivery determine the characteristics ofthese systems and their main goal the controlled release ofbioactive GFs
31 Synthesis Methods It is possible to found several pro-cedures to encapsulate hydrophilic molecules as proteinsor nucleic acids in polymeric nanomicroparticles Phase
separation [80] or spray drying [81] techniques have beenreported to encapsulate hydrophilic molecules However inthe case of proteins the most normally used procedureto encapsulate them into PLGA micro- and nanoparticlesis the double-emulsion (wateroilwater WOW) solventevaporation technique [75 82] A schematic description ofthis technique is presented in Figure 2 In a general wayPLGA is dissolved in an organic solvent and emulsified usingmechanical agitation or sonication with water containingan appropriate amount of protein Thus a primary wateroil(WO) emulsion is obtained In the second phase thisemulsion is poured into a large polar phase leading to animmediate precipitation of the particles as a consequenceof the polymer shrinkage around droplets of the primaryemulsion This phase may be composed of a water solutionof a stabilizer (surfactant) or ethanol-water mixtures [8384] After stirring the organic solvent is rapidly extractedby evaporation under vacuum A wide list of differentmodifications have been tested in this procedure in order toobtain a micronanocarrier system with adequate colloidalstability high encapsulation efficiency adequate bioactivityand finally a long-time release profilewith low ldquoinitial burstrdquo
6 BioMed Research International
The goal is to avoid a high amount of protein (gt60) beingreleased very quickly (24 hours) which is one of the biggestproblems of a controlled release system [76]
32 Organic Solvent Hans and Lowman show differentexamples of organic solvents used in multiple emulsionprocesses Normally dichloromethane (DMC) ethyl acetateacetone and their mixtures can be used [82] In the first stepa good organic solvent with low water solubility to facilitatethe emulsification process and low boiling point for an easyevaporation would be the election However the structureof the encapsulated protein molecules can be affected anddenaturation processes and loss of biological activity appearwhen they interact with a typical organic solvent as DMC[73] Ethyl acetate on the other hand exerts less denaturatingeffects with a lower incidence on the bioactivity of theencapsulated proteins [85]
Other important factors related with the organic solventare their physical properties that affect how the polymertails self-organize in the shell of the emulsion droplets andmodify the nanoparticle morphology and the encapsulationefficiency [86] In this way a higher water solubility ofthe organic solvent that is ethyl acetate favors a rapidsolvent removal Additionally the solvent removal rate canbe controlled by adjusting the volume of the polar phase aswell as the shear stress during the second emulsification stepAn increase of these two parameters increases the diffusionrate of ethyl acetate from primary microparticles to outeraqueous phase resulting in their rapid solidification [87] Italso enhances the encapsulation efficiency andminimizes thecontact-time between protein molecules and organic solvent[88] obtaining at the same time a lower burst effect and aslower drug release from the microparticles [87]
33 Particle Size and Morphology Particle size is an impor-tant parameter and one of the main goals of the deliverypolymeric system Microspheres from a few micrometers upto 100 120583m are suitable for oral delivery mucosal adhesion orinside scaffold use that is for bone regeneration Nanoscaledimension of the carrier offers enhanced versatility whencompared with particles of larger size This is due to thefact that they have higher colloidal stability improved dis-persibility and bioavailability more reactive surface and alsocan deliver proteins or drugs inside and outside of thecorresponding cells [89] BMP2 promotes bone formationand induces the expression of other BMPs and initiates thesignaling pathway from the cell surface by binding to twodifferent surface receptors [22] Therefore the BMP2 carrierparticles must release it into the extracellular medium Sincecellular intake of PLGAnanoparticles is very fast the intakingprocess can be limited by an increase in size from nano-to microparticles [90] However the interaction betweenparticles and cells is strongly influenced by particle size Ifcell internalization is desired the particle must be comprisedin the submicron scale at an interval between 2 and 500 nm[91] Moreover this size is needed for a rapid distributionafter parenteral administration in order to reach differenttissues through different biological barriers In addition
Figure 3 Scanning electron microscopy (SEM) photography ofPLGA nanoparticles obtained by a double emulsion emulsificationprocedureThis systemwith spherical shape low polydispersity andnanoscopic scale shows the intended properties for an adequatephysiological distribution and cell internalization
the intake by macrophages is minimized with a diameterof nanoparticles under 200 nm and even smaller [82 92]As discussed by Yang et al [93] slight modifications ofthe synthesis procedure can suppose drastic effects on thesize or particle morphology and therefore in the proteinencapsulation efficiency and kinetic release
In double emulsion processes the first emulsificationstep largely determines the particle size while the secondemulsification step characterized by the solvent eliminationand polymer precipitation mainly affects the particle mor-phology [86] However the use of surfactant solutions asthe polar medium of the second emulsification process andthe volume ratio between organic and polar phases in thisstep has shown an important influence in the final size [94]Therefore the correct election of the organic solvent thepolymer concentration the addition of surfactant and theemulsification energy allow controlling the size of the system
The incorporation of poloxamers (F68) in the organicsolvent of the primary emulsification helps to increase thecolloidal stability of the first dispersion by being placedat the wateroil interface This reduces the particle size incomparison with pure PLGA nanoparticles in which theonly stability source comes from electric charge of thecarboxyl groups of the PLGA [95] It is normal to obtainspherical micronanospheres with a polymeric porous coreA typical SEM micrograph of PLGA nanoparticles obtainedby WOW emulsion using a mixture of organic solvents(DCMacetone) and ethanolwater as second polar mediumis shown in Figure 3 in which the spherical shape anduniform size distribution are the main characteristics Theouter polymeric shell in the second emulsification steppushed the water droplets to the inner core according to theirsolidification process [96] This process allows producingparticles like capsules with a core-shell structure in whichthe inner core has a low polymer density Figure 4 shows atypical core-shell structure in which the polymer precipitatesand shrinks around the water droplets during the solventchange of the second phase and the subsequent organicsolvent evaporation process [97] In this case the process of
BioMed Research International 7
(a) (b)
Figure 4 PLGApoloxamers188 blend nanoparticles (a) Scanning transmission electron microscopy (STEM) photography (b) scanningelectron microscopy (SEM) photography STEM technique allows the analysis of the nanoparticle structure with an internal region with alow polymer density which is representative of nanocapsules with core-shell structure
solidification of the polymer is influenced and determined bythe miscibility of the organic solvent with the second polarphase and the removal rate
The polymeric shell often presents channels or pores asa consequence of the inner water extrusion due to osmoticforcesThis can reduce the encapsulation efficiency and favorsa fast initial leakage with the unwanted ldquoburst releaserdquo [93]This modification of internal structure of the particles isusually indicated assigning the term ldquonanosphererdquo to thesystem with a core consisting of a homogeneous polymermatrix The bioactive agent is dispersed within them whilethe core-shell structure would be similar to a ldquonanocapsulerdquowhere the biomolecule is preferably in the aqueous cavitysurrounded by the polymeric shell [78] (see Figure 2)
34 Stabilizer Agents
341 Colloidal Stability The double emulsion method nor-mally requires the presence of stabilizers in order to confercolloidal stability during the first emulsification step toprevent the coalescence of the emulsion droplets and latertomaintain the stability of the final nanomicroparticles [98]Polyvinyl alcohol (PVA) and PEO derivate as poloxamers(also named pluronics) have been used inmost cases [83 94]Others include natural surfactants such as phospholipids[99 100] In some cases it is possible to avoid surfactantsif the particles have an electrostatic stability contributionthat is from the uncapped end carboxyl groups of the PLGAmolecules [101]
As it has been previously commented PVA and polox-amers have shown their efficiency in synthetizing both nano-and microparticles affecting not only the stability of thesystems but also their size and morphology Thus a sizereduction effect has been found using PVA in the externalwater phase affecting at the same time the surface porosity
mainly in microsized particles [94] A comparative studybetween this and phospholipids (di-palmitoyl phosphaty-dilcholine DPPC) as stabilizers showed that DPPC couldbe a better emulsifier than PVA to produce nano- andmicroparticles With this method a much lower amount ofstabilizer was needed to obtain a similar size In the samestudy a higher porosity on the particle surface for the PVAemulsified nanospheres was shown [99]
On the other hand the combination of PLGA withpoloxamers has shown positive effects for the nano- andmicrosystems in terms of stability [102] The use of thesesurfactants in the first or second steps of the WOWemulsion procedure leads to different situations Thus ifpoloxamers are blended with PLGA in the organic phaseof the primary emulsification an alteration of the surfaceroughness is obtained However if these are added in theinner water phase an increase of porosity is found [83] Inaddition their inclusion in the polar phase of the secondemulsification step also generates hydrophilic roughnesssurfaces A quantification of this is shown in Figure 5 inwhich the electrophoretic mobility of both PLGA pure andPLGApluronic F68 nanoparticles is measured as a functionof the pH of the medium The observed dependence withthis parameter is a consequence of the weak acid characterof the PLGA carboxyl groups When poloxamer moleculesare present at the interface a systematic reduction ofmobilitywas found as a consequence of the increase in the surfaceroughness The hydrophilic surfactant chains spread outtowards the solvent originating a displacement of the shearplane and the consequent mobility reduction [95 101]
The final PLGA particle size is primarily controlled byelectrostatic forces and is not significantly affected by thepresence or nature of poloxamer stabilizers [101] The recog-nition of the nanocarriers by the mononuclear phagocyticsystem (MPS) can be significantly altered if the surface of
8 BioMed Research International
pH4 5 6 7 8 9
minus6
minus4
minus2
0
2
120583e
(V m
minus1
sminus1)
Figure 5 Electrophoretic mobility versus pH for PLGA nano-particles with different characteristics (998787) PLGA (◼) PLGApolox-amer188 blend and (∙) PLGA covered by Immuno-120574-globulin Thedifferent surface composition affects the electrokinetic behaviourof bare nanoparticles Surface charge values were screened by thepresence of nonionic surfactant as poloxamers or in a higherextension by the presence of antibody molecules attached on thesurface
colloidal particles is modified by using PEO block copoly-mer of the poloxamer molecules The steric barrier givenby these surfactant molecules prevents or minimizes theadsorption of plasma protein and decreases the recognitionby macrophages [103] The size of microspheres is alsounaffected by the coencapsulation of poloxamers The sys-tem containing poloxamer-PLGA blends drive to an innerstructure displaying small holes and cavities in relation withmicrospheres of pure PLGA with a compact matrix-typestructure [83]
Microparticles formulated by poloxamer in the secondpolar medium have completely different surface than thePVA ones almost without pores [94] A comparison betweendifferent poloxamers shows that the hydrophilic-lipophylicbalance (HBL) of the surfactant plays a crucial role determin-ing the surfactant-polymer interactions and controlling theporosity and roughness of the nano-microparticles [83 104]
In a similar manner to surfactants polymer character-istics like the hydrophobicity grade the molecular weightor the hydrolysis degradation rate can strongly influencethe particlemorphologyTherefore the polymer compositionof the particles greatly affects its structure and propertiesThis is why it is usual to use other polymers in order tomodify the behavior and application of the particles Inthis way polyethylene glycol (PEG) of different chain lengthis frequently used to modify the surface characteristicsWith PEG particles are more hydrophilic and with roughersurfaces which affects the MPS action by increasing thecirculating-time and half-life in vivo like the presence of PEOchains [105] Additionally PEG chains also provide colloidalstability via steric stabilization Pegylated-PLGA nano- ormicroparticles can be normally obtained by using in the
synthesis method PLGAPEG di- and triblock copolymers[58 59 75] Natural polymers as chitosan besides modifyingthe hydrophobicity-hydrophilicity ratio of the surface alsoconfer them a mucoadhesive character [106]
342 Encapsulation Efficiency and Bioactivity Furthermorethe use of stabilizers (surfactants or polymers) also influ-ences the encapsulation efficiency and the protein stabilityIn fact for the WOW solvent evaporation process thechlorinated organic solvent used for the first emulsificationcould degrade protein molecules encapsulated in this stepif they come into contact with the organicwater interfacecausing their aggregation or denaturation [107]Thepolymer-protein interaction the shear stress for the emulsificationprocess and the pH reduction derived from PLGA polymerdegradation can also produce the same situation with thesubsequent loss of biological activity of the encapsulatedbiomolecules Different strategies to prevent it have beenused For example an increase of the viscosity around proteinmolecules can help to isolate them from their microenviron-ment [108] In this way viscous products such as starch havebeen used to prevent protein instability [109] These authorscoencapsulate BMP2 with albumin inside starch microparti-cles using other biodegradable polymer poly-120576-caprolactoneinstead of PLGA The BMP2 retained its bioactivity Despitea low encapsulation rate beside an initial burst followedby an uncompleted release the amount of BMP2 neededat the beginning was lower [109] The combination of PEOsurfactants with PLGA (blended in the organic phase) canalso preserve the bioactivity of microencapsulated proteins[110] or nucleic acids [84]
However in most cases the coencapsulation of GFswith other biomolecules was the preferred strategy Therebyserum albumins (SA) have shown the capacity to limit theaggregation-destabilization of several proteins incited by thewaterorganic solvent interface of the primary emulsificationprocess [111 112] White et al encapsulated lysozyme insidePLGA-PEG microparticles In addition to the protectivefunction they also observed an important increase of theentrapment efficiency when human SA was coencapsulatedwith lysozyme and BMP2 [59] drsquoAngelo et al used heparinas stabilizer because it forms a specific complex with severalGFs stabilizes their tridimensional structure and promotestheir bioactivity An encapsulation efficiency of 35 wasincreased to 87 using bovine SA as a second stabilizer toencapsulate two natural proangiogenic growth factors insidePLGA-poloxamer blended nanoparticlesThe in vitro cellularassays showed the preservation of the biological activity ofGFs up to one month [56]
The use of more hydrophilic surfactants (poloxamers)or polymers (PEG) in the inner water phase or blendedwith PLGA in the organic phase of the primary emulsionreduces the interaction of encapsulated proteins with thehydrophobic PLGA matrix This prevents disrupting thestructure of the protein molecules and helps at the sametime to neutralize the acidity generated by the hydrolyticdegradation of the PLGA [113] In some cases the combina-tion of several stabilizers such as poloxamers trehalose andsodium bicarbonate has been shown to preserve the integrity
BioMed Research International 9
of encapsulated proteins but it also reduces the encapsulationefficiency [114]
As a general rule encapsulation efficiency increases withthe size of the particles [82] Additionally the adequate sta-bilization of the primary emulsion by amphiphilic polymersand a rapid solidification (precipitation) of polymer in thesecond step are favorable parameters for enhancing proteinentrapment efficiency in the WOW emulsion technique[87]
The tendency of BMP2 to interact with hydrophobicsurfacesmay decrease the loss of encapsulated protein duringthe extraction of the solvent phase This favors a higherentrapment but it lowers the later extraction [58] An optimalprotein encapsulation is obtained when pH of the internaland external water phases is near the isoelectric point of theprotein [92] Blanco and Alonso [83] observed a reductionin the protein encapsulation efficiency when poloxamer wascoencapsulated in the primary emulsion This highlights themain role played by the protein-polymer interaction in theencapsulation efficiency and the later release process How-ever too much emulsifier may also result in a reduction ofthe encapsulation efficiency [99] Therefore an equilibriumbetween the emulsification powder of the surfactant and theirconcentration is needed
35 Release Profile The release profile represents one of themost important characteristics of a nanomicro particulatecarrier system since their development has a main finalobjective the adequate release of the encapsulated bioactivemolecules to reach the desired clinical action
The release pattern of protein encapsulated in PLGAmicronanoparticles can present different behavior It ispossible to find a continuous release when the diffusionof the biomolecule is faster than the particle erosion Thisprocess involves a continuous diffusion of the protein fromthe polymer matrix before the PLGA particle is degradedin lactic and glycolic acid monomers by hydrolysis [74] Abiphasic release characterized by an initial burst at or nearthe particle surface followed by a second phase in whichprotein is progressively released by diffusion has also beendescribedThe second phase can be enhanced by bulk erosionof PLGA shell and matrix which results in an importantincrease of pores and channels [75] A third triphasic releaseprofile has been found when a lag release period occursafter initial burst and until polymer degradation starts [115]Finally it is possible to obtain an incomplete protein releaseas a consequence of additional factors related with theprotein-polymer interaction or protein instability Figure 6illustrates the different release profiles previously describedThe optimal carrier system should be capable of releasinga controlled concentration gradient of growth factors inthe appropriate time preventing or at least reducing orcontrolling the initial burst effect [116] A controlled initialburst followed by a sustained release significantly improvesthe in vivo bone regeneration [117ndash119]
Giteau et al [108] present an interesting revision on ldquoHowto achieve a sustained and complete release from PLGAmicroparticlesrdquo They begin by analyzing the influence of therelease medium and sampling method on the release profile
00
20
40
60
80
100
Cum
ulat
ive r
eleas
e of p
rote
in (
)
Time (h)500400300200100
Figure 6 Release profiles (I) BSA release from PLGA nanoparti-cles with high initial burst release (red dots line) biphasic modelcombining a moderate initial burst and a subsequent sustainedrelease (blue dash line) triphasicmodel with a lag of release betweenboth initial and sustained release phases (dash-dot green line)incomplete release
and highlight the significance of the centrifugation cleaningprocess or the releasemedium volume Adjusting to adequatevalues the centrifugation speed or the buffer volume itis possible to separate micronanoparticles from protein-containing release medium in a very easy wayThis allows forstable and reproducible release patterns On the other handto ensure a better protein release profile modification of themicroparticle formulation and microencapsulation processin order to preserve protein aggregation has to be performedProtein stability has to be maintained by preventing theformation of harmful medium For example the synthesisformulation can be modified to use more hydrophilic poly-mers since they have been shown to reduce the initial burstand to deliver bioactive proteins over long time periods
The most relevant strategies are referenced below Drugrelease from PLGA nanomicroparticles can be controlledby the polymer molecular weight and the relation betweenmonomers (lactideglycolide) so that an increase in gly-colic acid accelerates the weight loss of polymer due tothe higher hydrophilicity of the matrix [75] A mixtureof different PLGA nanoparticles obtained using 50 50 and75 50 latideglycolide ratio has shown a great potential forprotein drug delivery with a higher initial burst from PLGA50 50 A slow release period has been observed for PLGA75 50 encapsulating a glycoprotein (120572-1-antitrypsin) withclinic activity in some pulmonary diseases [60]
On the other hand a faster erosion of the microsphereswith reduction in the PLGA molecular weight due to thefacility of water penetration and the subsequent polymerdegradation has been described [83] Schrier et al workingwithmicrospheres prepared by wow using different types ofPLGA analyzed the important role of the molecular weightlactide-glycolide relation and acid residues [57]The amountof rhBMP2 adsorbed on the microparticle surface increased
10 BioMed Research International
with the hydrophobicity of the polymer At the same time therelease was in correlation with the degradation profile of thedifferent polymers [57]
Thus the use of more hydrophilic polymers reduces thehydrophobic protein-polymer interaction This effect favorsa more homogeneous distribution in the polymer matrixand increases the water uptake in the microspheres Thusthe release rate of rhBMP2 encapsulated in microspherescomposed by a PEG-PLGA di-block copolymer is increasedwith the PEG content of the polymer matrix [58] A similarresult was obtained using PLGA-PEG-PLGA triblock copoly-mers [59] In this case modifying the monomer relation(lactide-glycolide) in the PLGA and increasing the amountof PLGA-PEG-PLGA in the formulations the release profileof BMP-2 coencapsulated with human SA in microesphereswas adjustable Similarly the interaction of lysozyme withpoloxamer 188 before their encapsulation produces a sus-tained release over 3 weeks without any burst effect In thesame line using PLGA-PEG-PLGA as polymer a sustainedrelease of bioactive lysozime was extended over 45 days whenthe protein was complexed with poloxamer 188 previously tothe encapsulation [120] However the presence of PEG300 asan additive of the inner phase of microparticles during theencapsulation process also influences the protein distributionand the release profile In this case there is a decrease of theinitial burst but with less overall release [58]
On the other hand the use of PLGA-poloxamers blendsis useful to obtain a sustained release for more than onemonthwithout any incidence in the high initial burst [56 92]However for an encapsulated plasmid inside nanoparticlesobtained by PLGA-poloxamer blends the hydrophobicity ofthe surfactant allows prolonging the release up to 2 weeks in acontrolledmannerMoreover a complete release was reachedfor the PLGA-poloxamer blend instead PLGA nanoparticlesin which the maximum release was around 40 [84]
PLGA and poloxamers (pluronic F68) blends can also beused to obtain nanocomposite vesicles by a double emulsionprocess These vesicles are suitable for the encapsulation ofhydrophobic and hydrophilic molecules The presence ofpluronic affects the colloidal stability of the vesicles and therelease pattern of the encapsulated molecules These vesiclespresent a wall of 30 nm and the drug is encapsulated in thepresence of the poloxamer [121]
Other strategies include the use of different compounds toincrease the release timeThus BMP2 encapsulated in PLGA-PVA nanoparticles (around 300 nm) showed higher encapsu-lation efficiency and a short-time release profile with a veryhigh initial burst However with the same synthesis proce-dure (wow) but using PHBV (Poly(3 hydroxybutyrateco-3-hydroxivalerate)) BMP7 loaded nanocapsules had lessencapsulation efficiency despite a long-time delivery Nev-ertheless the maximum released amount was lower Thisdifference in the release profile was due to the differencein hydrophilicity and degradation rates of both polymers[122] Similarly PLGA-poloxamer blend nanoparticles weresuperficially modified by introducing chitosan in the secondstep of the synthesisThis method showed a sustained releaseprofile for up to 14 days without any initial important burstIn this case a recombinant hepatitis B antigen was used
[106] Moreover the use of heparin conjugated with PLGAporous microspheres has also been described to obtain along-time delivery system reducing at the same time theinitial burst In these systems heparin was immobilized ontothe nanomicroparticle surface The release was controlledby using the binding affinities of heparin to several growthfactors including BMP2 In this case the initial burst wasreduced to 4ndash7 during first day followed by a sustainedrelease of about 1 per day [51ndash53]
The initial burst release may be attenuated by thefabrication of double-wall microspheres that is core-shellmicroparticles The presence of a PLA shell reduces therelease rate of BSA encapsulated in the PLGA core andextends the duration of the release profile up to two monthsMoreover an increase in the PLA molecular weight influ-ences the rate of particle erosion which further slows theprotein release [123]
The modification of the viscosity in the environmentof microparticles additionally influences the release patternViscosity can control the burst at earliest time point andpromote a sustained release This situation has been shownfor rhBMP2-PLGA microspheres embedded in a chitosan-thioglycolic acid hydrogel (Poloxamer 407) [124] Yilgor etal also incorporated the nanoparticles of their sequentialdelivery system into a scaffold composed by chitosan andchitosan-PEO [54] In other work PLGAPVA microsphereswith encapsulated BMP2 were combined with differentcomposite biomaterials (gelatin hydrogel or polypropylenefumarate) The sustained release of the bioactive moleculewas extended over a period of 42 days In vivo results indicatethe importance of the composite characteristics In this casean enhanced bone formation was obtained when the PLGAmicroparticles were incorporated into the more hydrophobicmatrix (polypropylene fumarate) [125 126]
Finally Table 2 summarizes important information aboutdifferent parameters related to the use of PLGA basednano- ormicroparticles to encapsulate transport and releasegrowth factors (mainly BMP2)
36 Gene Therapy for Bone Tissue Engineering DirectedDelivery In the last years gene therapy has begun to playa role in bone tissue regeneration becoming an alternativemethod for the delivery of BMP2 [127 128] Thus the genesencoding a specific protein can be delivered to a specific cellrather than the proteins themselves To reach this purposean efficient gene vector is necessary Viral vectors possess thebest transfection efficiency but numerous disadvantages themost notable of them being the risk of mutagenesis Nonviralvectors elude these problems but with a significant reductionin the transfection rate [129]Therefore intracellular deliveryof bioactive agents has become the most used strategy forgene therapy looking for the adequate transfection andconsequent expression of the desired protein [79]
PLGA microspheres obtained by a wow double emul-sion process have been used by Qiao et al to entrap plasmid-BMP2polyethyleneimine nanoparticles In this case a sus-tained release of these nanoparticles until 35 days without ini-tial burst was found resulting in differentiation of osteoblast
BioMed Research International 11
Table 2 Nanomicroparticles systems to encapsulate GFs mainly BMP2 growth factor Most of them are in the microscopic scale andwere used to be entrapped into scaffold of different characteristics PVA has been the more used surfactant-stabilizer It is possible to findboth encapsulation and surface adsorption of the growth factors with high-moderate efficiency The use of heparin as stabilizer reducessignificantly the initial burst release favoring a sustained release in the time The bioactivity of the GF was preserved in most of the systemsand coencapsulation with other biomolecules seems to have a similar effect than the use of surfactants as stabilizers
Polymers Stabilizer Size Encapsulation EE Release Biological activity Reference
PLGA PVA 10ndash20120583m AdsorbedrhBMP2
20 ngmL ofconstant sustained
release
Better boneformation after 8
weeksFu et al 2013 [44]
PLGA PVA 10ndash100120583m rhBMP2-BSA69 (BMP)
Burst (20)Sustained until77 (28 days)
BMP2 moleculeswith bioactivity Tian et al 2012 [45]
PLGA 75 25 PVA 182120583m 82 mdash
Good bonedefect repair
outcomes within8minus12 weeks
Rodrıguez-Evoraet al 2014 [46]
PLGA PVA 228120583m 605
30 initial burstSlower release of4 per week After
8 weeks 60released
No loss ofbioactivity
Reyes et al 2013[47]
PLGAPEGNo doubleemulsionsynthesis
100ndash200 120583m Adsorbed BMP2
13 initial burstSlower release of001ndash8 per dayAfter 23 days 70
released
Substantial boneregeneration of the
scaffold
Rahman et al 2014[48]
Different PLGA PVA 20ndash100 120583m
30 (uncappedPLGA)
90 (cappedPLGA)
26ndash49 (1 day)Total after 2 weeks
No loss ofbioactivity
Lupu-Haber et al2013 [49]
PLGA 75 25 PVA 5ndash125 120583m mdashInitial burst 30 (1
day)Sustained 35 days
Higher volumesand surface areacoverage of new
bone
Wink et al 2014[50]
PLGA Heparin 200ndash800 nm Adsorbed BMP294
No initial burstSustained over 4
weeks
Significantreduction of theBMP2 dose forgood boneformation
La et al 2010 [51]
PLGA Heparin-Poloxamer 160 nm Adsorbed BMP2
100
Initial burst(4ndash7) linear
profile
Higher matrixmineralization ofregenerated bone
Chung et al 2007[52]
PLGA Heparin 100ndash250 nm Adsorbed 94Initial burst 10 (1
day)60 after 30 days
No loss ofbioactivityEfficacy of
administrationamount 50-fold
lower
Jeon et al 2008 [53]
PLGA PVA sim300 nm 80 85 initial burst (1day)
No loss ofbioactivity
Yilgor et al 2009[54]
PLGA (in rings) PVA 215 120583m 66Moderate burstSustained releaseover 6 weeks
60 of calvariadefect were healed
Rodrıguez-Evoraet al 2013 [55]
PLGA-Poloxamer 188Blend
Poloxamer 150 nmFGF-BSA-Heparin60ndash80
40 initial burst (1day) 60 (30 days)
No loss ofbioactivity
drsquoAngelo et al 2010[56]
Different PLGApolymers PVA 120583m order
rhBMP2adsorption40ndash75
20ndash80 initialburst (1 day) mdash Schrier et al 2001
[57]
12 BioMed Research International
Table 2 Continued
Polymers Stabilizer Size Encapsulation EE Release Biological activity Reference
PLGAPEG PVA 37ndash67 120583m 72ndash99 33 initial burst (1day)
Little loss ofbioactivity
Lochmann et al2010 [58]
PLGAPLGA-PEG-PLGA PVA 100 120583m HSA-BMP2
6070 initial burst (1
day)No loss ofbioactivity
White et al 2013[59]
PLGA PVA 100ndash1000 nm Α-1-antitrypsin90
30 initial burst (1day)
50 after 24 days
Biological activitywas preservedusing BSA and120573-cyclodextrine
Pirooznia et al2012 [60]
promoted by the correct transfection of the delivered bio-functional BMP2-DNA [130]
In spite of the general caution with gene therapy thegenetic delivery of BMP2 has the potentiality of a better safetycompared with the delivery of large amounts of recombinantprotein [131] Lu et al specify the urgent need to developmoreefficient delivery nanoparticles and transfection methods inorder to apply the nonviral vectors in stem cell engineeringand bone regeneration Although enhanced bone formationhas been shown in several recent studies using genes suchas HIF-1120572 and miRNAs new genetic sequences will bediscovered and used in bone engineering in the near futurethat will most likely change our perspective [132]
PLGA nanospheres represent a well-studied biomoleculedelivery system that could be applied to cell targeting inorder to enhance the delivery of specific proteins or nucleicacids inside or near the bone engineering reference cells thatis mesenchymal stem cells [133]The targeting properties canbe supplied by a ligand functionalization strategy modifica-tion of the surface structure of the nanocarrier by conjugatinga cell-specific ligand to direct the release of encapsulatedbiomolecules preferably in close association with the targetcells [134]The use of pegylated nanoparticles with a covalentattachment of different ligands is reported as a potentialtechnique to deliver bone cell-specific biomolecules for boneengineering [135]
Specific antibodies that recognize surface receptors inthese cells could be covalently coupled to the surface of PLGAnanoparticles obtaining ldquoimmunonanoparticlesrdquo There areseveral examples of antibody immobilization on surfaceof PLGA nanoparticles Kocbek et al demonstrated thespecific recognition of breast tumor cells by a specific mono-clonal antibody attached on PLGA fluorescent nanoparticlesobtained by WOW emulsion process [136] For the surfacecovalent attachment they used a more simple carbodiimidemethod which promotes the formation of an amide bondbetween free carboxylic end groups of PLGA nanoparticlesand primary amine groups of the antibody molecule [81]This procedure can be highly influenced by the presenceof stabilizers frequently used to confer colloidal stabilityto nanoparticles The electrophoretic mobility of PLGAnanoparticles with an antibody (immuno-120574-globuline anti-human C-reactive protein) covalently attached on the surfaceis shown in Figure 5 It is necessary to remark the drasticdecrease in the mobility values of the antibody-modified
nanoparticles with respect to bare PLGA nanoparticleswhich could imply low colloidal stability and the subse-quent aggregation of the nanosystem Santander-Ortega etal proposed a lower antibody loading in which the barePLGA patches must be coated by a nonionic surfactant inorder to obtain immunoreactive stable nanoparticles [95]Ratzinger et al indicated that the presence of high polox-amer concentrations decreased the coupling efficiency tocarboxylic end groups in PLGA nanoparticles showing thatan equilibrium that combines sufficient stability and the bestcoupling efficiency is necessary [98] To prevent this problemCheng et al synthetized carboxyl functionalized PLGA-PEG block copolymer attaching a specific aptamer to thesurface of pegylated nanoparticles via carbodiimide methodIn this work an enhanced drug delivery to prostate tumorshas been shown in comparison to equivalent nontargetednanoparticles [137]
37 Scaffolds The data reported in the literature indicatethat PLGA micronanoparticles are promising to achievea sustained spatial and temporally controlled delivery ofgrowth factors required for cell growth and cell differen-tiation They can be incorporated with cells in solid scaf-fold or injectable hydrogels [73] Scaffolds are porous 3Dstructures normally used to improve tissue-engineered bone[28] According to Tian et al [45] a scaffold designedwith this objective must have (1) appropriate mechanicalstrength to support the growth of new bone (2) appropriateporosity to allow ingrowth of bone-related cells (3) goodbiocompatibility allowing the growth of cells on its surfacewithout being rejected by the body and (4) low toxicity tocells and tissues surrounded and (5) must be able to induceosteogenic differentiation of bone-related stem cells and (6)be biodegradable with nontoxic degradation products thatcan be eventually replaced by new bone Additionally thescaffold for bone regeneration must maintain the delivery orrelease of BMP (growth factors) ldquoin siturdquo for a long time Inthis way nanomicroparticles inside scaffolds are being usedto release an adequate flow of these signaling biomoleculesand preserve their functional structure [138] The incorpo-ration of colloidal micronanoparticles into fibrous scaffoldsadds in the possibility of multiple drugs loading Howeverthis multidrug system could also involve a decrease ofthe mechanical properties of the structure and a possibleloss of nanoparticles entrapped between the fibers [139]
BioMed Research International 13
Considering that the in vivo half-life of most biomoleculesespecially proteins is relatively short it is essential thatbioactive scaffolds maintain a desired concentration ldquoin siturdquoto direct tissue regeneration To do so an initial release ofthe encapsulated growth factor in the first hours to quicklyget an effective therapeutic concentration followed by asustained long-time release profile is required [139] Most ofthe polymeric particles inserted in scaffold structures are ina micron-scale The main objective of these microparticlesis the protection and temporary control of growth factordelivery However given the porosity of these structuresnanoparticles and especially particles of a few microns maybecome more important since it is possible to design systemswith a simple and easy diffusion through the structure Thisprocess could allow the specific recognition of a particularcell type releasing their encapsulated BMPs in the sameenvironment and helping their differentiation to cellbonetissue In any case the larger-size microspheres might notnecessarily be useless for bone regeneration scaffolds As themicrospheres gradually degrade the space they occupied willbe conducive to ingrowth of tissue In addition to affectingthe compressionmodulus of scaffolds because of their hollowfeature the particle size of microspheres can also influencethe release of rhBMP2 [45]
4 Conclusion
The use of polymeric particles using PLGA is a promisingsystem for a spatially and temporally controlled delivery ofgrowth factors that promote cell growth and differentiationin bone engineering and regeneration by means of theirincorporation beside cells into solid scaffold or hydrogels
The PLGA is widely used for its biodegradability andbiocompatibility and is approved by FDA and the EuropeanMedicines Agency for use in drug delivery systems suppliedvia parenteral On the other hand BMPs are potent growthfactors for bone repair and specifically BMP2 shows excellentability to induce bone formation of adequate quality Theprocedure for synthesizing PLGA nano- or microparticlescan be modified in their different variables to obtain systemswith controlled size in which it is possible to encapsulatehydrophobic or hydrophilic molecules with an adequate col-loidal stability and the possibility of surface functionalizationfor targeted delivery
With this scenario an optimization of methods and com-ponentsmust balance the structure andmorphology of PLGAmicronanoparticles in order to achieve high encapsulationefficiency of BMP2 and looking for a main goal control ofdelivery reducing the initial burst and reaching a sustainedrelease profile preserving the biological activity and directedto the target cells tominimize the clinical amount needed andallowing a correct bone tissue regeneration
Conflict of Interests
The authors declare no conflict of interests with any of theproducts listed in the paper
Acknowledgments
The authors wish to express their appreciation for thefinancial support granted by the ldquoMinisterio de Educaciony Cienciardquo (MEC Spain) Projects MAT2013-43922-R andResearch Groups no FQM-115 no CTS-138 and no CTS-583 (Junta de Andalucıa Spain) Partial support was alsoprovided by the Andalucıa Talent Hub Program from theAndalusian Knowledge Agency cofunded by the EuropeanUnionrsquos Seventh Framework Program Marie Skłodowska-Curie actions (COFUND Grant Agreement no 291780) andthe Ministry of Economy Innovation Science and Employ-ment of the Junta de Andalucıa (Miguel Padial-Molina)
References
[1] M Padial-Molina P Galindo-Moreno and G Avila-OrtizldquoBiomimetic ceramics in implant dentistryrdquoMinerva Biotecno-logica vol 21 no 3 pp 173ndash186 2009
[2] B Al-Nawas and E Schiegnitz ldquoAugmentation proceduresusing bone substitute materials or autogenous bonemdasha sys-tematic review and meta-analysisrdquo European Journal of OralImplantology vol 7 supplement 2 pp S219ndashS234 2014
[3] A Katranji P Fotek and H-L Wang ldquoSinus augmentationcomplications etiology and treatmentrdquo Implant Dentistry vol17 no 3 pp 339ndash349 2008
[4] C E Misch ldquoMaxillary sinus augmentation for endostealimplants organized alternative treatment plansrdquo The Interna-tional Journal of Oral Implantology Implantologist vol 4 no 2pp 49ndash58 1987
[5] C Myeroff and M Archdeacon ldquoAutogenous bone graft donorsites and techniquesrdquo The Journal of Bone amp Joint SurgerymdashAmerican Volume vol 93 no 23 pp 2227ndash2236 2011
[6] G Avila R Neiva C E Misch et al ldquoClinical and histologicoutcomes after the use of a novel allograft for maxillary sinusaugmentation a case seriesrdquo Implant Dentistry vol 19 no 4pp 330ndash341 2010
[7] S J Froum S S Wallace N Elian S C Cho and D P TarnowldquoComparison of mineralized cancellous bone allograft (Puros)and anorganic bovine bonematrix (Bio-Oss) for sinus augmen-tation histomorphometry at 26 to 32 weeks after graftingrdquoTheInternational Journal of Periodontics amp Restorative Dentistryvol 26 no 6 pp 543ndash551 2006
[8] P Galindo-Moreno G Avila J E Fernandez-Barbero et alldquoEvaluation of sinus floor elevation using a composite bone graftmixturerdquo Clinical Oral Implants Research vol 18 no 3 pp 376ndash382 2007
[9] P Galindo-Moreno I Moreno-Riestra G Avila et al ldquoEffectof anorganic bovine bone to autogenous cortical bone ratioupon bone remodeling patterns following maxillary sinusaugmentationrdquo Clinical Oral Implants Research vol 22 no 8pp 857ndash864 2011
[10] S L Wheeler ldquoSinus augmentation for dental implants theuse of alloplastic materialsrdquo Journal of Oral and MaxillofacialSurgery vol 55 no 11 pp 1287ndash1293 1997
[11] S S Wallace and S J Froum ldquoEffect of maxillary sinusaugmentation on the survival of endosseous dental implantsA systematic reviewrdquo Annals of Periodontologythe AmericanAcademy of Periodontology vol 8 no 1 pp 328ndash343 2003
14 BioMed Research International
[12] M Padial-Molina and H F Rios ldquoStem cells scaffolds andgene therapy for periodontal engineeringrdquo Current Oral HealthReports vol 1 no 1 pp 16ndash25 2014
[13] M Padial-Molina S L Volk and H F Rios ldquoPeriostinincreases migration and proliferation of human periodontalligament fibroblasts challenged by tumor necrosis factor -alphaand Porphyromonas gingivalis lipopolysaccharidesrdquo Journal ofPeriodontal Research vol 49 no 3 pp 405ndash414 2014
[14] H Behnia A Khojasteh M Soleimani A Tehranchi and AAtashi ldquoRepair of alveolar cleft defect with mesenchymal stemcells and platelet derived growth factors a preliminary reportrdquoJournal of Cranio-Maxillofacial Surgery vol 40 no 1 pp 2ndash72012
[15] M Padial-Molina J T Marchesan A D Taut Q Jin WV Giannobile and H F Rios ldquoMethods to validate tooth-supporting regenerative therapiesrdquo Methods in Molecular Biol-ogy vol 887 pp 135ndash148 2012
[16] M R Urist ldquoBone formation by autoinductionrdquo Science vol150 no 3698 pp 893ndash899 1965
[17] P Boyne and S D Jones ldquoDemonstration of the osseoinductiveeffect of bone morphogenetic protein within endosseous dentalimplantsrdquo Implant Dentistry vol 13 no 2 pp 180ndash184 2004
[18] E A Wang V Rosen J S DrsquoAlessandro et al ldquoRecombinanthuman bone morphogenetic protein induces bone formationrdquoProceedings of the National Academy of Sciences of the UnitedStates of America vol 87 no 6 pp 2220ndash2224 1990
[19] J M Wozney ldquoThe bone morphogenetic protein family andosteogenesisrdquoMolecular Reproduction andDevelopment vol 32no 2 pp 160ndash167 1992
[20] E Barboza A Caula and F Machado ldquoPotential of recombi-nant human bone morphogenetic protein-2 in bone regenera-tionrdquo Implant Dentistry vol 8 no 4 pp 360ndash367 1999
[21] A C Carreira G G Alves W F Zambuzzi M C Sogayarand J M Granjeiro ldquoBone morphogenetic proteins structurebiological function and therapeutic applicationsrdquo Archives ofBiochemistry and Biophysics vol 561 pp 64ndash73 2014
[22] J C Bustos-Valenzuela A Fujita E Halcsik J M Granjeiroand M C Sogayar ldquoUnveiling novel genes upregulated by bothrhBMP2 and rhBMP7 during early osteoblastic transdifferen-tiation of C2C12 cellsrdquo BMC Research Notes vol 4 article 3702011
[23] K Tsuji A Bandyopadhyay B D Harfe et al ldquoBMP2 activityalthough dispensable for bone formation is required for theinitiation of fracture healingrdquo Nature Genetics vol 38 no 12pp 1424ndash1429 2006
[24] K Tsuji K Cox A Bandyopadhyay B D Harfe C J Tabin andV Rosen ldquoBMP4 is dispensable for skeletogenesis and fracture-healing in the limbrdquo The Journal of Bone and Joint SurgerymdashAmerican Volume vol 90 supplement 1 pp 14ndash18 2008
[25] K Tsuji K Cox L Gamer D Graf A Economides and VRosen ldquoConditional deletion of BMP7 from the limb skeletondoes not affect bone formation or fracture repairrdquo Journal ofOrthopaedic Research vol 28 no 3 pp 384ndash389 2010
[26] G Chen C Deng and Y-P Li ldquoTGF-beta and BMP signalingin osteoblast differentiation and bone formationrdquo InternationalJournal of Biological Sciences vol 8 no 2 pp 272ndash288 2012
[27] M-C Ramel and C S Hill ldquoSpatial regulation of BMP activityrdquoFEBS Letters vol 586 no 14 pp 1929ndash1941 2012
[28] A C Carreira F H Lojudice E Halcsik R D Navarro M CSogayar and J M Granjeiro ldquoBone morphogenetic proteinsfacts challenges and future perspectivesrdquo Journal of DentalResearch vol 93 no 4 pp 335ndash345 2014
[29] F Deschaseaux L Sensebe and D Heymann ldquoMechanisms ofbone repair and regenerationrdquo Trends in Molecular Medicinevol 15 no 9 pp 417ndash429 2009
[30] T DMueller and J Nickel ldquoPromiscuity and specificity in BMPreceptor activationrdquo FEBS Letters vol 586 no 14 pp 1846ndash1859 2012
[31] C Sieber J Kopf C Hiepen and P Knaus ldquoRecent advancesin BMP receptor signalingrdquo Cytokine amp Growth Factor Reviewsvol 20 no 5-6 pp 343ndash355 2009
[32] G Sapkota C Alarcon F M Spagnoli A H Brivanlou and JMassague ldquoBalancing BMP signaling through integrated inputsinto the Smad1 linkerrdquoMolecular Cell vol 25 no 3 pp 441ndash4542007
[33] W F McKay S M Peckham and J M Badura ldquoA comprehen-sive clinical review of recombinant human bonemorphogeneticprotein-2 (INFUSE Bone Graft)rdquo International Orthopaedicsvol 31 no 6 pp 729ndash734 2007
[34] P Hong D Boyd S D Beyea and M Bezuhly ldquoEnhancementof bone consolidation in mandibular distraction osteogenesisa contemporary review of experimental studies involving adju-vant therapiesrdquo Journal of Plastic Reconstructive and AestheticSurgery vol 66 no 7 pp 883ndash895 2013
[35] D B Spagnoli and R E Marx ldquoDental implants and the use ofrhBMP-2rdquo Dental Clinics of North America vol 55 no 4 pp883ndash907 2011
[36] M Nevins C Kirker-HeadM Nevins J AWozney R Palmerand D Graham ldquoBone formation in the goat maxillary sinusinduced by absorbable collagen sponge implants impregnatedwith recombinant human bone morphogenetic protein-2rdquo TheInternational Journal of Periodontics amp Restorative Dentistryvol 16 no 1 pp 8ndash19 1996
[37] P J Boyne L C Lilly R E Marx et al ldquoDe novo bone induc-tion by recombinant human bone morphogenetic protein-2(rhBMP-2) in maxillary sinus floor augmentationrdquo Journal ofOral and Maxillofacial Surgery vol 63 no 12 pp 1693ndash17072005
[38] L Torrecillas-Martinez A Monje M A Pikos et al ldquoEffect ofrhBMP-2 uponmaxillary sinus augmentation a comprehensivereviewrdquo Implant Dentistry vol 22 no 3 pp 232ndash237 2013
[39] J Lee C Susin N A Rodriguez et al ldquoSinus augmentationusing rhBMP-2ACS in a mini-pig model relative efficacy ofautogenous fresh particulate iliac bone graftsrdquo Clinical OralImplants Research vol 24 no 5 pp 497ndash504 2013
[40] RG TriplettMNevins R EMarx et al ldquoPivotal randomizedparallel evaluation of recombinant human bonemorphogeneticprotein-2absorbable collagen sponge and autogenous bonegraft for maxillary sinus floor augmentationrdquo Journal of Oraland Maxillofacial Surgery vol 67 no 9 pp 1947ndash1960 2009
[41] D W K Kao A Kubota M Nevins and J P Fiorellini ldquoThenegative effect of combining rhBMP-2 and Bio-Oss on boneformation for maxillary sinus augmentationrdquoThe InternationalJournal of Periodontics amp Restorative Dentistry vol 32 no 1 pp61ndash67 2012
[42] O Hanisch D N Tatakis M D Rohrer P S Wohrle JM Wozney and U M E Wikesjo ldquoBone formation andosseointegration stimulated by rhBMP-2 following subantralaugmentation procedures in nonhuman primatesrdquoThe Interna-tional Journal of Oral ampMaxillofacial Implants vol 12 no 6 pp785ndash792 1997
[43] K Wada A Niimi K Watanabe T Sawai and M UedaldquoMaxillary sinus floor augmentation in rabbits a comparative
BioMed Research International 15
histologic-histomorphometric study between rhBMP-2 andautogenous bonerdquo The International Journal of Periodontics ampRestorative Dentistry vol 21 no 3 pp 253ndash263 2001
[44] R Fu S Selph M McDonagh et al ldquoEffectiveness and harmsof recombinant human bone morphogenetic protein-2 in spinefusion a systematic review and meta-analysisrdquo Annals of Inter-nal Medicine vol 158 no 12 pp 890ndash902 2013
[45] Z Tian Y Zhu J Qiu et al ldquoSynthesis and characterization ofUPPE-PLGA-rhBMP2 scaffolds for bone regenerationrdquo Journalof Huazhong University of Science and TechnologymdashMedicalScience vol 32 no 4 pp 563ndash570 2012
[46] M Rodrıguez-Evora E Garcıa-Pizarro C del Rosario et alldquoSmurf1 knocked-down mesenchymal stem cells and BMP-2 in an electrospun system for bone regenerationrdquo Biomacro-molecules vol 15 no 4 pp 1311ndash1322 2014
[47] R Reyes A Delgado R Solis et al ldquoCartilage repair bylocal delivery of TGF-1205731 or BMP-2 from a novel segmentedpolyurethanepolylactic-co-glycolic bilayered scaffoldrdquo Journalof Biomedical Materials Research Part A 2013
[48] C V Rahman D Ben-David A Dhillon et al ldquoControlledrelease of BMP-2 from a sintered polymer scaffold enhancesbone repair in a mouse calvarial defect modelrdquo Journal of TissueEngineering and Regenerative Medicine vol 8 no 1 pp 59ndash662014
[49] Y Lupu-Haber O Pinkas S Boehm T Scheper C Kasper andM Machluf ldquoFunctionalized PLGA-doped zirconium oxideceramics for bone tissue regenerationrdquoBiomedicalMicrodevicesvol 15 no 6 pp 1055ndash1066 2013
[50] J D Wink P A Gerety R D Sherif et al ldquoSustaineddelivery of rhBMP-2 by means of poly(lactic-co-glycolic acid)microspheres cranial bone regeneration without heterotopicossification or craniosynostosisrdquo Plastic and ReconstructiveSurgery vol 134 no 1 pp 51ndash59 2014
[51] W-G La S-W Kang H S Yang et al ldquoThe efficacy of bonemorphogenetic protein-2 depends on its mode of deliveryrdquoArtificial Organs vol 34 no 12 pp 1150ndash1153 2010
[52] Y-I Chung K-M Ahn S-H Jeon S-Y Lee J-H Lee and GTae ldquoEnhanced bone regeneration with BMP-2 loaded func-tional nanoparticle-hydrogel complexrdquo Journal of ControlledRelease vol 121 no 1-2 pp 91ndash99 2007
[53] O Jeon S J Song H S Yang et al ldquoLong-term deliveryenhances in vivo osteogenic efficacy of bone morphogeneticprotein-2 compared to short-term deliveryrdquo Biochemical andBiophysical Research Communications vol 369 no 2 pp 774ndash780 2008
[54] P Yilgor K Tuzlakoglu R L Reis N Hasirci and V HasircildquoIncorporation of a sequential BMP-2BMP-7 delivery systeminto chitosan-based scaffolds for bone tissue engineeringrdquoBiomaterials vol 30 no 21 pp 3551ndash3559 2009
[55] M Rodrıguez-Evora A Delgado R Reyes et al ldquoOsteogeniceffect of local long versus short term BMP-2 delivery froma novel SPU-PLGA-120573TCP concentric system in a critical sizedefect in ratsrdquo European Journal of Pharmaceutical Sciences vol49 no 5 pp 873ndash884 2013
[56] I drsquoAngelo M Garcia-Fuentes Y Parajo et al ldquoNanoparticlesbased on PLGA poloxamer blends for the delivery of proangio-genic growth factorsrdquoMolecular Pharmaceutics vol 7 no 5 pp1724ndash1733 2010
[57] J A Schrier B F Fink J B Rodgers H C Vasconez and PP DeLuca ldquoEffect of a freeze-dried CMCPLGA microspherematrix of rhBMP-2 on bone healingrdquo AAPS PharmSciTech vol2 no 3 article E18 2001
[58] A Lochmann H Nitzsche S von Einem E Schwarz and KMader ldquoThe influence of covalently linked and free polyethy-lene glycol on the structural and release properties of rhBMP-2loaded microspheresrdquo Journal of Controlled Release vol 147 no1 pp 92ndash100 2010
[59] L J White G T S Kirby H C Cox et al ldquoAcceleratingprotein release from microparticles for regenerative medicineapplicationsrdquoMaterials Science and Engineering C Materials forBiological Applications vol 33 no 5 pp 2578ndash2583 2013
[60] N Pirooznia S Hasannia A S Lotfi and M Ghanei ldquoEncap-sulation of alpha-1 antitrypsin in PLGA nanoparticles in vitrocharacterization as an effective aerosol formulation in pul-monary diseasesrdquo Journal of Nanobiotechnology vol 10 article20 2012
[61] M Ronga A Fagetti G Canton E Paiusco M F Surace andP Cherubino ldquoClinical applications of growth factors in boneinjuries experience with BMPsrdquo Injury vol 44 supplement 1pp S34ndashS39 2013
[62] J G Devine J R Dettori J C France E Brodt and R AMcGuire ldquoThe use of rhBMP in spine surgery is there a cancerriskrdquo Evidence-Based Spine-Care Journal vol 3 no 2 pp 35ndash41 2012
[63] E J Carragee E L Hurwitz and B KWeiner ldquoA critical reviewof recombinant human bone morphogenetic protein-2 trials inspinal surgery emerging safety concerns and lessons learnedrdquoThe Spine Journal vol 11 no 6 pp 471ndash491 2011
[64] M C Simmonds J V E Brown M K Heirs et al ldquoSafetyand effectiveness of recombinant human bone morphogeneticprotein-2 for spinal fusion a meta-analysis of individual-participant datardquo Annals of Internal Medicine vol 158 no 12pp 877ndash889 2013
[65] V H-Y Chung A Y-L Chen L-B Jeng C-C Kwan S-H Cheng and S C-N Chang ldquoEngineered autologous bonemarrow mesenchymal stem cells alternative to cleft alveolarbone graft surgeryrdquo Journal of Craniofacial Surgery vol 23 no5 pp 1558ndash1563 2012
[66] T A Ratko S E Belinson D J Samson C Bonnell K MZiegler and N Aronson BoneMorphogenetic ProteinThe Stateof the Evidence of On-Label and Off-Label Use Agency forHealthcare Research and Quality Rockville Md USA 2010
[67] G Barratt ldquoColloidal drug carriers achievements and perspec-tivesrdquo Cellular and Molecular Life Sciences vol 60 no 1 pp 21ndash37 2003
[68] V W Bramwell and Y Perrie ldquoParticulate delivery systems forvaccinesrdquo Critical Reviews inTherapeutic Drug Carrier Systemsvol 22 no 2 pp 151ndash214 2005
[69] N Csaba M Garcia-Fuentes and M J Alonso ldquoThe perfor-mance of nanocarriers for transmucosal drug deliveryrdquo ExpertOpinion on Drug Delivery vol 3 no 4 pp 463ndash478 2006
[70] M J Santander-Ortega T Stauner B Loretz et al ldquoNanopar-ticles made from novel starch derivatives for transdermal drugdeliveryrdquo Journal of Controlled Release vol 141 no 1 pp 85ndash922010
[71] W Jiang R K Gupta M C Deshpande and S P Schwende-man ldquoBiodegradable poly(lactic-co-glycolic acid) microparti-cles for injectable delivery of vaccine antigensrdquo Advanced DrugDelivery Reviews vol 57 no 3 pp 391ndash410 2005
[72] T R Shantha Kumar K Soppimath and S K NachaegarildquoNovel delivery technologies for protein and peptide therapeu-ticsrdquo Current Pharmaceutical Biotechnology vol 7 no 4 pp261ndash276 2006
16 BioMed Research International
[73] F Danhier E Ansorena J M Silva R Coco A Le Bretonand V Preat ldquoPLGA-based nanoparticles an overview ofbiomedical applicationsrdquo Journal of Controlled Release vol 161no 2 pp 505ndash522 2012
[74] A Kumari S K Yadav and S C Yadav ldquoBiodegradablepolymeric nanoparticles based drug delivery systemsrdquo Colloidsand Surfaces B Biointerfaces vol 75 no 1 pp 1ndash18 2010
[75] H K Makadia and S J Siegel ldquoPoly Lactic-co-Glycolic Acid(PLGA) as biodegradable controlled drug delivery carrierrdquoPolymers vol 3 no 3 pp 1377ndash1397 2011
[76] F Mohamed and C F van der Walle ldquoEngineering biodegrad-able polyester particles with specific drug targeting and drugrelease propertiesrdquo Journal of Pharmaceutical Sciences vol 97no 1 pp 71ndash87 2008
[77] G A Silva O P Coutinho P Ducheyne andR L Reis ldquoMateri-als in particulate form for tissue engineering 2 Applications inbonerdquo Journal of Tissue Engineering and Regenerative Medicinevol 1 no 2 pp 97ndash109 2007
[78] S Zhang and H Uludag ldquoNanoparticulate systems for growthfactor deliveryrdquoPharmaceutical Research vol 26 no 7 pp 1561ndash1580 2009
[79] V E Santo M E Gomes J F Mano and R L Reis ldquoFromnano-to macro-scale nanotechnology approaches for spatiallycontrolled delivery of bioactive factors for bone and cartilageengineeringrdquo Nanomedicine vol 7 no 7 pp 1045ndash1066 2012
[80] M-K Tran A Swed and F Boury ldquoPreparation of polymericparticles in CO
2medium using non-toxic solvents formulation
and comparisons with a phase separation methodrdquo EuropeanJournal of Pharmaceutics and Biopharmaceutics vol 82 no 3pp 498ndash507 2012
[81] B Ertl F Heigl M Wirth and F Gabor ldquoLectin-mediatedbioadhesion preparation stability andCaco-2 binding of wheatgerm agglutinin-functionalized poly-(DL-lactic-co-glycolicacid)-microspheresrdquo Journal of Drug Targeting vol 8 no 3 pp173ndash184 2000
[82] M L Hans and A M Lowman ldquoBiodegradable nanoparticlesfor drug delivery and targetingrdquo Current Opinion in Solid Stateand Materials Science vol 6 no 4 pp 319ndash327 2002
[83] D Blanco and M J Alonso ldquoProtein encapsulation and releasefrom poly(lactide-co-glycolide) microspheres effect of the pro-tein and polymer properties and of the co-encapsulation ofsurfactantsrdquo European Journal of Pharmaceutics and Biophar-maceutics vol 45 no 3 pp 285ndash294 1998
[84] N Csaba P Caamano A Sanchez F Domınguez and MJ Alonso ldquoPLGA poloxamer and PLGApoloxamine blendnanoparticles new carriers for gene deliveryrdquo Biomacro-molecules vol 6 no 1 pp 271ndash278 2005
[85] C Sturesson and J Carlfors ldquoIncorporation of protein inPLG-microspheres with retention of bioactivityrdquo Journal ofControlled Release vol 67 no 2-3 pp 171ndash178 2000
[86] I D Rosca F Watari and M Uo ldquoMicroparticle formationand its mechanism in single and double emulsion solventevaporationrdquo Journal of Controlled Release vol 99 no 2 pp271ndash280 2004
[87] F T Meng G H Ma W Qiu and Z G Su ldquoWOW doubleemulsion technique using ethyl acetate as organic solventeffects of its diffusion rate on the characteristics of micropar-ticlesrdquo Journal of Controlled Release vol 91 no 3 pp 407ndash4162003
[88] R Ghaderi and J Carlfors ldquoBiological activity of lysozyme afterentrapment in poly (dl-lactide-co-glycolide)-microspheresrdquoPharmaceutical Research vol 14 no 11 pp 1556ndash1562 1997
[89] H Wang S C G Leeuwenburgh Y Li and J A Jansen ldquoTheuse of micro- and nanospheres as functional components forbone tissue regenerationrdquo Tissue Engineering Part B Reviewsvol 18 no 1 pp 24ndash39 2012
[90] S Xiong X Zhao B C Heng K W Ng and J S-C LooldquoCellular uptake of Poly-(DL-lactide-co-glycolide) (PLGA)nanoparticles synthesized through solvent emulsion evapora-tion and nanoprecipitation methodrdquo Biotechnology Journal vol6 no 5 pp 501ndash508 2011
[91] L Y T Chou K Ming and W C W Chan ldquoStrategies forthe intracellular delivery of nanoparticlesrdquo Chemical SocietyReviews vol 40 no 1 pp 233ndash245 2011
[92] M J Santander-Ortega M V Lozano-Lopez D Bastos-Gonzalez J M Peula-Garcıa and J L Ortega-Vinuesa ldquoNovelcore-shell lipid-chitosan and lipid-poloxamer nanocapsulesstability by hydration forcesrdquo Colloid and Polymer Science vol288 no 2 pp 159ndash172 2010
[93] Y-Y Yang T-S Chung and N Ping Ng ldquoMorphology drugdistribution and in vitro release profiles of biodegradable poly-meric microspheres containing protein fabricated by double-emulsion solvent extractionevaporation methodrdquo Biomateri-als vol 22 no 3 pp 231ndash241 2001
[94] T Feczko J Toth and J Gyenis ldquoComparison of the prepara-tion of PLGA-BSA nano- and microparticles by PVA polox-amer and PVPrdquo Colloids and Surfaces A Physicochemical andEngineering Aspects vol 319 no 1ndash3 pp 188ndash195 2008
[95] M J Santander-Ortega D Bastos-Gonzalez and J L Ortega-Vinuesa ldquoElectrophoretic mobility and colloidal stability ofPLGA particles coated with IgGrdquo Colloids and Surfaces BBiointerfaces vol 60 no 1 pp 80ndash88 2007
[96] Y-Y Yang H-H Chia and T-S Chung ldquoEffect of prepara-tion temperature on the characteristics and release profiles ofPLGA microspheres containing protein fabricated by double-emulsion solvent extractionevaporation methodrdquo Journal ofControlled Release vol 69 no 1 pp 81ndash96 2000
[97] D-L Fang Y Chen B Xu et al ldquoDevelopment of lipid-shell andpolymer core nanoparticles with water-soluble salidroside foranti-cancer therapyrdquo International Journal ofMolecular Sciencesvol 15 no 3 pp 3373ndash3388 2014
[98] G Ratzinger U Langer L Neutsch F Pittner M Wirth and FGabor ldquoSurface modification of PLGA particles the interplaybetween stabilizer ligand size and hydrophobic interactionsrdquoLangmuir vol 26 no 3 pp 1855ndash1859 2010
[99] S-S Feng and G Huang ldquoEffects of emulsifiers on thecontrolled release of paclitaxel (Taxol) from nanospheres ofbiodegradable polymersrdquo Journal of Controlled Release vol 71no 1 pp 53ndash69 2001
[100] JMChan L ZhangK P Yuet et al ldquoPLGA-lecithin-PEGcore-shell nanoparticles for controlled drug deliveryrdquo Biomaterialsvol 30 no 8 pp 1627ndash1634 2009
[101] M Fraylich W Wang K Shakesheff C Alexander andB Saunders ldquoPoly(DL-lactide-co-glycolide) dispersions con-taining pluronics from particle preparation to temperature-triggered aggregationrdquo Langmuir vol 24 no 15 pp 7761ndash77682008
[102] M J Santander-Ortega J M Peula-Garcıa F M Goycooleaand J L Ortega-Vinuesa ldquoChitosan nanocapsules effect ofchitosan molecular weight and acetylation degree on electroki-netic behaviour and colloidal stabilityrdquo Colloids and Surfaces BBiointerfaces vol 82 no 2 pp 571ndash580 2011
[103] J S Tan D E Butterfield C L Voycheck K D Caldwell and JT Li ldquoSurfacemodification of nanoparticles by PEOPPOblock
BioMed Research International 17
copolymers to minimize interactions with blood componentsand prolong blood circulation in ratsrdquo Biomaterials vol 14 no11 pp 823ndash833 1993
[104] C Bouissou U Potter H Altroff H Mardon and C van derWalle ldquoControlled release of the fibronectin central cell bindingdomain from polymeric microspheresrdquo Journal of ControlledRelease vol 95 no 3 pp 557ndash566 2004
[105] R Gref Y Minamitake M T Peracchia V Trubetskoy VTorchilin and R Langer ldquoBiodegradable long-circulating poly-meric nanospheresrdquo Science vol 263 no 5153 pp 1600ndash16031994
[106] P Paolicelli C Prego A Sanchez and M J Alonso ldquoSurface-modified PLGA-based nanoparticles that can efficiently asso-ciate and deliver virus-like particlesrdquo Nanomedicine vol 5 no6 pp 843ndash853 2010
[107] I Brigger C Dubernet and P Couvreur ldquoNanoparticles incancer therapy and diagnosisrdquoAdvancedDrugDelivery Reviewsvol 54 no 5 pp 631ndash651 2002
[108] A Giteau M C Venier-Julienne A Aubert-Pouessel and J PBenoit ldquoHow to achieve sustained and complete protein releasefrom PLGA-based microparticlesrdquo International Journal ofPharmaceutics vol 350 no 1-2 pp 14ndash26 2008
[109] E R Balmayor G A Feichtinger H S Azevedo Mvan Griensven and R L Reis ldquoStarch-poly-120576-caprolactonemicroparticles reduce the needed amount of BMP-2rdquo ClinicalOrthopaedics and Related Research vol 467 no 12 pp 3138ndash3148 2009
[110] M J Santander-Ortega D Bastos-Gonzalez J L Ortega-Vinuesa andM J Alonso ldquoInsulin-loaded PLGA nanoparticlesfor oral administration an in vitro physico-chemical character-izationrdquo Journal of Biomedical Nanotechnology vol 5 no 1 pp45ndash53 2009
[111] L Meinel O E Illi J Zapf M Malfanti H Peter Merkleand B Gander ldquoStabilizing insulin-like growth factor-I inpoly(DL-lactide-co-glycolide) microspheresrdquo Journal of Con-trolled Release vol 70 no 1-2 pp 193ndash202 2001
[112] C Srinivasan Y K Katare T Muthukumaran and A K PandaldquoEffect of additives on encapsulation efficiency stability andbioactivity of entrapped lysozyme from biodegradable polymerparticlesrdquo Journal of Microencapsulation vol 22 no 2 pp 127ndash138 2005
[113] M Tobıo S P Schwendeman Y Guo J McIver R Langerand M J Alonso ldquoImproved immunogenicity of a core-coatedtetanus toxoid delivery systemrdquoVaccine vol 18 no 7-8 pp 618ndash622 1999
[114] D K Malik S Baboota A Ahuja S Hasan and J Ali ldquoRecentadvances in protein and peptide drug delivery systemsrdquoCurrentDrug Delivery vol 4 no 2 pp 141ndash151 2007
[115] J L Cleland ldquoProtein delivery from biodegradable micro-spheresrdquo in Protein Delivery Physical Systems L M Sandersand R W Hendron Eds pp 1ndash41 Plenum Press New YorkNY USA 1997
[116] S H Oh T H Kim and J H Lee ldquoCreating growth factorgradients in three dimensional porous matrix by centrifugationand surface immobilizationrdquo Biomaterials vol 32 no 32 pp8254ndash8260 2011
[117] B N Brown J E Valentin A M Stewart-Akers G P McCabeand S F Badylak ldquoMacrophage phenotype and remodelingoutcomes in response to biologic scaffolds with and without acellular componentrdquo Biomaterials vol 30 no 8 pp 1482ndash14912009
[118] B N Brown J M Freund L Han et al ldquoComparison of threemethods for the derivation of a biologic scaffold composed ofadipose tissue extracellularmatrixrdquoTissue EngineeringmdashPart CMethods vol 17 no 4 pp 411ndash421 2011
[119] B Li T Yoshii A E Hafeman J S Nyman J C Wenkeand S A Guelcher ldquoThe effects of rhBMP-2 released frombiodegradable polyurethanemicrosphere composite scaffoldson new bone formation in rat femorardquo Biomaterials vol 30 no35 pp 6768ndash6779 2009
[120] A Paillard-Giteau V T Tran O Thomas et al ldquoEffect ofvarious additives and polymers on lysozyme release fromPLGAmicrospheres prepared by an sow emulsion techniquerdquoEuropean Journal of Pharmaceutics and Biopharmaceutics vol75 no 2 pp 128ndash136 2010
[121] B P Nair and C P Sharma ldquoPoly(lactide-co-glycolide)-laponite-F68 nanocomposite vesicles through a single-stepdouble-emulsion method for the controlled release of doxoru-bicinrdquo Langmuir vol 28 no 9 pp 4559ndash4564 2012
[122] P Yilgor N Hasirci and V Hasirci ldquoSequential BMP-2BMP-7 delivery from polyester nanocapsulesrdquo Journal of BiomedicalMaterials ResearchmdashPart A vol 93 no 2 pp 528ndash536 2010
[123] Y Xia Q Xu C-H Wang and D W Pack ldquoProtein encapsu-lation in and release from monodisperse double-wall polymermicrospheresrdquo Journal of Pharmaceutical Sciences vol 102 no5 pp 1601ndash1609 2013
[124] Y Fu L Du QWang et al ldquoIn vitro sustained release of recom-binant human bone morphogenetic protein-2 microspheresembedded in thermosensitive hydrogelsrdquo Die Pharmazie vol67 no 4 pp 299ndash303 2012
[125] D H R Kempen L Lu T E Hefferan et al ldquoRetention ofin vitro and in vivo BMP-2 bioactivities in sustained deliveryvehicles for bone tissue engineeringrdquo Biomaterials vol 29 no22 pp 3245ndash3252 2008
[126] D H R Kempen L Lu A Heijink et al ldquoEffect of localsequential VEGF andBMP-2 delivery on ectopic and orthotopicbone regenerationrdquo Biomaterials vol 30 no 14 pp 2816ndash28252009
[127] H Nie M-L Ho C-K Wang C-H Wang and Y-C FuldquoBMP-2 plasmid loaded PLGAHAp composite scaffolds fortreatment of bone defects in nude micerdquo Biomaterials vol 30no 5 pp 892ndash901 2009
[128] F Wegman Y van der Helm F C Oner W J A Dhert andJ Alblas ldquoBone morphogenetic protein-2 plasmid DNA as asubstitute for bone morphogenetic protein-2 protein in bonetissue engineeringrdquo Tissue Engineering Part A vol 19 no 23-24pp 2686ndash2692 2013
[129] J Fischer A Kolk S Wolfart et al ldquoFuture of local boneregenerationmdashprotein versus gene therapyrdquo Journal of Cranio-Maxillofacial Surgery vol 39 no 1 pp 54ndash64 2011
[130] C Qiao K Zhang H Jin et al ldquoUsing poly(lactic-co-glycolicacid) microspheres to encapsulate plasmid of bone morpho-genetic protein 2polyethylenimine nanoparticles to promotebone formation in vitro and in vivordquo International Journal ofNanomedicine vol 8 pp 2985ndash2995 2013
[131] C H Evans ldquoGene delivery to bonerdquo Advanced Drug DeliveryReviews vol 64 no 12 pp 1331ndash1340 2012
[132] C-H Lu Y-H Chang S-Y Lin K-C Li andY-CHu ldquoRecentprogresses in gene delivery-based bone tissue engineeringrdquoBiotechnology Advances vol 31 no 8 pp 1695ndash1706 2013
[133] TNVo F K Kasper andAGMikos ldquoStrategies for controlleddelivery of growth factors and cells for bone regenerationrdquo
18 BioMed Research International
Advanced Drug Delivery Reviews vol 64 no 12 pp 1292ndash13092012
[134] W Ji H Wang J J J P van den Beucken et al ldquoLocal deliveryof small and large biomolecules in craniomaxillofacial bonerdquoAdvanced Drug Delivery Reviews vol 64 no 12 pp 1152ndash11642012
[135] V Luginbuehl L Meinel H P Merkle and B Gander ldquoLocal-ized delivery of growth factors for bone repairrdquo EuropeanJournal of Pharmaceutics and Biopharmaceutics vol 58 no 2pp 197ndash208 2004
[136] P Kocbek N Obermajer M Cegnar J Kos and J Kristl ldquoTar-geting cancer cells using PLGA nanoparticles surface modifiedwith monoclonal antibodyrdquo Journal of Controlled Release vol120 no 1-2 pp 18ndash26 2007
[137] J Cheng B A Teply I Sherifi et al ldquoFormulation of func-tionalized PLGA-PEG nanoparticles for in vivo targeted drugdeliveryrdquo Biomaterials vol 28 no 5 pp 869ndash876 2007
[138] C Romagnoli F DrsquoAsta andM L Brandi ldquoDrug delivery usingcomposite scaffolds in the context of bone tissue engineeringrdquoClinical Cases inMineral and BoneMetabolism vol 10 no 3 pp155ndash161 2013
[139] D Puppi X Zhang L Yang F Chiellini X Sun and EChiellini ldquoNanomicrofibrous polymeric constructs loadedwith bioactive agents and designed for tissue engineeringapplications a reviewrdquo Journal of Biomedical Materials ResearchPart B Applied Biomaterials vol 102 no 7 pp 1562ndash1579 2014
Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595
Contents lists available at ScienceDirect
Colloids and Surfaces B Biointerfaces
jo ur nal ho me p ag e wwwelsev ier com locate co lsur fb
Full Length Article
Dual delivery nanosystem for biomolecules Formulationcharacterization and in vitro release
Inmaculada Ortega-Oller a1 Teresa del Castillo-Santaella b1 Miguel Padial-Molina aPablo Galindo-Moreno a Ana Beleacuten Joacutedar-Reyes b Joseacute Manuel Peula-Garciacutea bclowast
a Department of Oral Surgery and Implant Dentistry University of Granada Granada Spainb Biocolloid and Fluid Physics Group Department of Applied Physics University of Granada 18071 Granada Spainc Department of Applied Physics II University of Malaga 29071 Malaga Spain
a r t i c l e i n f o
Article historyReceived 25 May 2017Received in revised form 18 July 2017Accepted 17 August 2017
KeywordsPLGANanoparticlesProtein encapsulationRelease
a b s t r a c t
Because of the biocompatible and biodegradable properties of poly (lactic-co-glycolic acid) (PLGA)nanoparticles (NPs) based on this polymer have been widely studied for drugbiomolecule delivery andlong-term sustained-release In this work two different formulation methods for lysozyme-loaded PLGANPs have been developed and optimized based on the double-emulsion (wateroilwater WOW) sol-vent evaporation technique They differ mainly in the phase in which the surfactant (Pluronicreg F68) isadded water (W-F68) and oil (O-F68) The colloidal properties of these systems (morphology by SEM andSTEM hydrodynamic size by DLS and NTA electrophoretic mobility temporal stability in different mediaprotein encapsulation release and bioactivity) have been analyzed The interaction surfactant-proteindepending on the formulation procedure has been characterized by surface tension and dilatational rhe-ology Finally cellular uptake by human mesenchymal stromal cells and cytotoxicity for both systemshave been analyzed
Spherical hard NPs are made by the two methods However in one case they are monodisperse withdiameters of around 120 nm (O-F68) and in the other case a polydisperse system of NPs with diametersbetween 100 and 500 nm is found (W-F68) Protein encapsulation efficiency release and bioactivity aremaintained better by the W-F68 formulation method This multimodal system is found to be a promisingldquodual deliveryrdquo system for encapsulating hydrophilic proteins with strong biological activity at the cell-surface and cytoplasmic levels
copy 2017 Elsevier BV All rights reserved
1 Introduction
Tissue regeneration is a complex biological action involvingmultiple steps in a sequential ordered and controlled manner [12]Classically bioactive molecules have been proposed to aid in theseprocesses However the use of high doses denaturation and lossof biological activity uncontrolled timing of action and diffusionto other tissues have been highlighted as major issues of this ther-apeutic strategy [3] To help solve these problems nanomedicinehas been intensively investigated in recent years as an emerging
lowast Corresponding author at Department of Applied Physics II University of Maacutelaga29071 Maacutelaga Spain
E-mail address jmpeulaumaes (JM Peula-Garciacutea)1 Both authors contributed equally to this work
area This involves diagnostic therapeutic and regeneration meth-ods by means of structures and systems in which size and shape arecontrolled at the atomic molecular and supramolecular levels [4]The transport and controlled delivery of drugs andor therapeuticbiomolecules improve their pharmacokinetics and pharmacody-namics and at the same time minimize harmful side effects Forthese purposes different nanosystems have been described Polylactic-co-glycolic acid (PLGA) exhibits low cytotoxicity as well ashigh biocompatibility and biodegradability with the release of non-toxic by-products [5]
In the last decade the use of PLGA has been investigated todeliver a wide spectrum of active agents from hydrophobic drugmolecules [6ndash8] to hydrophilic biomolecules as peptides [9] pro-teins [10ndash15] or nucleic acids [1617] These delivery systemshave been produced via different formulation processes for theirapplication in both systemic and local site-specific therapies [18]
httpdxdoiorg101016jcolsurfb2017080270927-7765copy 2017 Elsevier BV All rights reserved
I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595 587
However their design and development as nanocarriers are diffi-cult due to the problematic release pattern when the encapsulatedmolecules are proteins for which initial bursts and slow or incom-plete release might be a problem [18ndash20] Moreover the specificconditions of the release may need to be different depending onthe final application of the nanocarrier [2021]
The water-in-oil-in-water (WOW) double emulsion techniqueis the most widely used protein-encapsulation method for PLGAmicro- (MP) and nanoparticles (NP) [2223] It allows differentfactors to be modulated such as the type of PLGA the use ofother polymers blended with PLGA the addition of surfactants themechanical stress or the organic solvent [20] It is also possible toconstruct several types of co-polymers to modify the hydropho-bicityhydrophilicity ratio [1824] and the colloidal stability sizeand release process PLGApolyethylene glycol pair and surfactantssuch as polyvinyl alcohol (PVA) or polyethylene oxides (PEO) arethe most widely studied [7122526]
On the other hand tissue engineering requires the participa-tion of mesenchymal stromal cells (MSCs) [27] MSCs are known tohave the ability to differentiate into multiple cell types includingosteoblasts Osteoblasts are the main cells responsible for synthe-sizing the mineralized compartment of bone tissue This processis regulated by among other molecules BMP-2 [3] PLGA parti-cles loaded with BMP-2 have been extensively used as has beendescribed and reviewed elsewhere [328ndash31]
Thus within this context it was the aim of the current study tooptimize the formulation and properties of a nanoparticle systemwith potential therapeutic applications Two different strategies toobtain PLGA-surfactant NPs were tested by using lysozyme as amodel for BMP-2 The size and morphology polydispersity indexzeta potential colloidal stability and encapsulation efficiency (EE)of the protein were analyzed
Once the physico-chemical characterization was completed thestudy was focused on the protein-release process using differ-ent techniques to study the results of in vitro experiments andfocusing it on the release pattern and the biological activity of thelysozyme released In this way a new formulation was establishedto develop a PLGA nanosystem with a singular dual size distribu-tion and the adequate balance between encapsulation and releaseof biologically active proteins Finally the effects of the proposedPLGA system were tested on primary MSCs in vitro as a proof ofconcept
2 Materials and methods
21 Formulation of the nanoparticles
Poly(lactide-co-glycolide) acid (PLGA 5050) ([C2 H2 O2]x [C3H4 O2]y) x = 50 y = 50 (Resomerreg 503H) 32ndash44 kDa was used asthe polymer The polymeric surfactant Pluronicreg F68 (Poloxamer188) (Sigma-Aldrich) was used as the emulsifier The structureis based on a poly(ethylene oxide)-block-poly(propylene oxide)-block-poly(ethylene oxide) and it is expressed as PEOa-PPOb-PEOawith a = 75 and b = 30 Lysozyme from chicken egg white (Sigma-L7651) was used as hydrophilic protein Water was purified ina Milli-Q Academic Millipore system Two different formulationmethods were developed termed O-F68 and W-F68
In the O-F68 method 25 mg of PLGA and 15 mg of F68 were dis-solved in 660 L of dichloromethane (DMC) and vortexed Then330 L of acetone were added and vortexed Next 100 L of abuffered solution at pH 128 with or without lysozyme (5 mgmL)were added dropwise while vortexing for 30 s Immediately thisprimary wateroil (WO) emulsion was poured into a glass con-taining 125 mL of ethanol under magnetic stirring and 125 mLof MilliQ water were added After 10 min of magnetic stirring the
organic solvents were rapidly extracted by evaporation under vac-uum until the sample reached a final volume of 10 mL
In the W-F68 method 100 mg of PLGA were dissolved in a tubecontaining 1 mL of ethyl acetate (EA) and vortexed 40 L of abuffered solution at pH 128 with or without lysozyme (20 mgmL)were added and immediately sonicated (Branson Ultrasonics 450Analog Sonifier) fixing the Duty cycle dial at 20 and the Outputcontrol dial at 4 for 1 min with the tube surrounded by ice This pri-mary WO emulsion was poured into a plastic tube containing 2 mLof a buffered solution (pH 128) of F68 at 1 mgmL and vortexingfor 30 s Then the tube surrounded by ice was sonicated again atthe maximum amplitude for the micro tip (Output control 7) for1 min This second WOW emulsion was poured into a glass con-taining 10 mL of the buffered F68 solution and kept under magneticstirring for 2 min The organic solvent was then rapidly extractedby evaporation under vacuum to a final volume of 8 mL
22 Cleaning and storage
After the organic solvent evaporation the sample was cen-trifuged for 10 min at 20 C at 14000 or 12000 rpm for O-F68 andW-F68 methods respectively The supernatant was filtered using100 nm filters for measuring the free non-encapsulated protein Thepellet was then resuspended in PB up to a final volume of 4 mL andkept under refrigeration at 4 C
221 Protein loading and encapsulation efficiencyThe initial protein loading was optimized for the nanoparticle
formulation preserving the final colloidal stability after the evapo-ration step and being different for each nanosystem Also 16 ww(LysPLGA) was used for O-F68 and 08 ww (LysPLGA) for W-F68one The amount of encapsulated lysozyme was calculated by mea-suring the difference between the initial amount added and the freenon-encapsulated protein which was tested by bicinchoninic acidassay (BCA Sigma-Aldrich) Then protein encapsulation efficiency(EE) and final drug loading (DL) was calculated as follows
EE = MI minus MF
MItimes 100 DL = MI minus MF
Mpolymertimes 100
where MI the initial total mass of Lys MF is the total mass of Lys inthe aqueous supernatant and Mpolymer is the mass of PLGA in theformulation
23 Characterization of the nanoparticles
231 Interfacial characterization of the first water-in-oilemulsion
The surface tension and dilatational rheology measurementsat the air-water interface were made in the OCTOPUS [32] aPendant Drop Surface Film Balance equipped with a subphasemulti-exchange device (patent submitted P201001588) describedin detail elsewhere [33] Here air plays the role of the organic phaseThe surface tension is calculated with DINATENreg software basedon axisymmetric drop shape analysis (ADSA) and the dilatationalmodulus (E) of the interfacial layer is determined from image anal-ysis with the program CONTACTOreg The in vitro model is describedin ldquoSupplementary materialrdquo
232 Particle morphologyNanoparticles were imaged by Scanning Electron Microscopy
(SEM) and Scanning Transmission Electron Microscopy (STEM)using a Zeiss SUPRA 40VP field emission scanning electron micro-scope from the Centre for Scientific Instrumentation of theUniversity of Granada (CIC UGR)
588 I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595
233 Nanoparticle size and electrokinetic mobilityThe hydrodynamic diameter and electrophoretic mobility of the
NPs were determined by using a Zetasizer NanoZeta ZS device(Malvern Instrument Ltd UK) working at 25 C with a He-Ne laserof 633 nm and a scattering angle of 173 Each data point wastaken as an average over three independent sample measurementsThe size of the NPs was characterized by Dynamic Light Scattering(DLS) The average hydrodynamic diameter (Z-average or cumu-lant mean) and the polydispersity index (PDI) were computedThese parameters are calculated through a cumulant analysis ofthe data which is applicable for narrow monomodal size distribu-tions [34] We also determined the intensity size distribution froman algorithm provided by the Zetasizer software (General Purpose)
The electrophoretic mobility was determined by the techniqueof Laser Doppler Electrophoresis An electrophoretic mobility dis-tribution as well as an average electrophoretic mobility (-average)was established for each sample
The hydrodynamic size distribution of the NPs with wide sizedistributions from DLS was also measured by using Nanoparti-cle Tracking Analysis (NTA) in a NanoSight LM10-HS(GB) FT14(NanoSight Amesbury United Kingdom) All samples were mea-sured more than three times for 60 s with manual shutter gainbrightness and threshold adjustments at 25 C The average sizedistribution (particle concentration vs diameter) was calculatedas an average of at least three independent size distributions
234 Nuclear magnetic resonance (NMR) of the nanoparticlesThe 1HNMR spectra of free F68 lysozyme-loaded particles from
O-F68 method with and without F68 and lysozyme-loaded parti-cles from W-F68 method were measured with a VNMRS 500 MHzspectrometer (Agilent) in the Centre for Scientific Instrumentation(CIC) of the University of Granada
24 Colloidal and temporal stability in biological media
The average hydrodynamic diameter and the polydispersityindex (PDI) by DLS of each system were measured to determinetheir colloidal stability in different media (Phosphate buffer [PB]Phosphate buffer saline [PBS] and cell culture medium Dulbeccorsquosmodified Eaglersquos medium [DMEM] from Sigma) and at differenttimes after (0 1 and 5 days)
In vitro release experiments were conducted following a simi-lar methodology as described above (Encapsulation efficiency) butusing 1 mL of each sample suspended in PBS at 37 C The proteinreleased from these samples was determined every 24 h by super-natant analysis and the pellet was suspended in the same volumeof buffer to maintain the release conditions All experiments weredeveloped in triplicate
241 Confocal microscopyLysozyme was labeled with fluorescein isothiocyanate (FITC)
using a method described by Kok et al [35] After FITC and lysozymecovalent conjugation concentrations were estimated spectropho-tometrically using the extinction coefficients described for FITC at494 nm and 280 nm The lysozyme concentration was calculatedmeasuring optical absorbance at 280 nm and subtracting the cor-responding FITC absorbance at this wavelength Images were madein a Nikon A1 laser scanning confocal microscope from CIC UGRAll experiments were performed in triplicate and replicated at leasttwice
25 Biological activity and interactions
251 Lysozyme biological activityThe biological activity of lysozyme was analyzed by an enzy-
matic activity kit (Sigma-Aldrich) using Micrococcus lysodeikticuscells as the substrate following the manufacturerrsquos instructions
252 Cellular uptakePrimary human mesenchymal stem cells (hMSCs) were taken
from healthy maxillary alveolar bone according to previouslydescribed protocols [36] After confirming their phenotype byflow cytometry and trilineage differentiation tests 12000 cellsper well were cultivated in sterile plates with glass bottom(Ibidi cat n 81158) overnight These cells were treated withmedium without fetal bovine serum (FBS) and Cell Tracker Red(15000) (C34552 ThermoFisher) for 30 min Then the mediumwas removed and supplemented with 10 FBS after which theparticles with lysozyme-FITC were added Then the hMSCs wereincubated 30 min again washed three times with PBS 1X and freshmedium supplemented with 2 FBS added Finally the hMSCs wereexamined by a confocal microscope (Nikon Eclipse Ti-E) Cell cul-tures were in all cases maintained at 37 C and 5 CO2 atmosphere
3 Results and discussion
31 Formulation of the nanoparticles
The methods developed in this work are intended to improvethe existing formulation techniques for hydrophilic protein loaded-PLGA NPs based on a double-emulsion process [1022] The noveltyof these methods is the use of the polymeric surfactant F68 either inthe organic phase (O-F68 method) or in the aqueous phase (W-F68)This surfactant reduces the size of the NPs enhances their stabilityand protects the encapsulated protein In addition the presence ofF68 on the surface of the particles reduces the recognition of thenanocarriers by the mononuclear phagocytic system (MPS) [37]
Additionally the choice of the organic solvent significantlyaffects the properties of the final colloidal system since the organicsolvent solubility regulates the inner and surface structure of theparticle In addition the interaction of the solvent with the encap-sulated biomolecule can alter its bioactivity as a consequence ofits denaturation as found for methylene chloride [26] In the O-F68method DMC is chosen as the organic solvent due to its lower watersolubility to facilitate the emulsification process and its low boil-ing point for easy evaporation However a freely water-miscibleorganic solvent (acetone) and the emulsifier F68 were added inthis organic phase to reduce its negative biological effects on theencapsulated protein [24] This emulsifier also reduces the protein-hydrophobic PLGA matrix interaction and thus the disruption ofthe protein structure [3] By contrast in the W-F68 method ethylacetate was used as the organic solvent which exerts less denatur-izing effects on the encapsulated protein [38] The higher watersolubility of this solvent favors rapid solvent removal The sol-vent removal rate is also accelerated by increasing the shear stressduring the second emulsification step It also enhances the encap-sulation efficiency and minimizes the contact time between theprotein and organic solvent [3] Poloxamer F68 is introduced in theexternal aqueous phase
Both formulations (O-F68 and W-F68) (Table 1) gave rise to col-loidally stable samples and the encapsulation of lysozyme insidethe nanoparticles in agreement with the double WOW emulsionmethod [23] Lysozyme was chosen as a model protein due to itsbiostability well-known characteristics and ease in quantifying itsbiological activity [3940] In addition its molecular size (143 kD)and its basic isoelectric point (around pH = 11) make it an appro-
I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595 589
Table 1Formulation conditions and protein encapsulation results PLGA F68 and LYSI are the initial amount of polymer surfactant and lysozyme respectively Initial is the initialpolymer-protein rate in ww EE is the encapsulation efficiency LYSF is the final encapsulated amount of lysozyme DL is the final drug loading rate in ww
PLGA (mg) F68 (mg) LYSI (mg) Initial EE LYSF (mg) DL
O-F68-Lys 25 15 04 16 625 025 1W-F68-Lys 100 2 08 08 731 058 058
priate model for other proteins such as bone-growth factors [15]Three main objectives drove the optimization of the appropriaterelation among the polymer poloxamer and protein (1) to havecolloidally stable nanosystems of submicron sizes (2) to encap-sulate a sufficient amount of protein and (3) to prevent proteindestabilization by maintaining their biological activity
Therefore regardless of the formulation method it wasintended to limit the initial protein loading to provide colloidallystable nanosystems In our case as shown in Table 1 Initial val-ues were the best choice to maintain colloidal stability withoutsignificantly changing the size distribution (see below) In con-sequence DL presents relative low values for both formulationsalthough the encapsulated amount of lysozyme LYSF is greaterthan those required for therapeutic proteins with lower clinicallyeffective amounts [41] The value of EE found for O-F68-Lys NPsis in consonance with the formulation characteristics and simi-lar to other reports with different proteins [12104214] includingbovine serum albumin (BSA) or insulin [1242] and several growthfactors [14]
The presence of surfactant stabilizes the emulsion droplets andreduces their size However it also alters the protein-polymerinteraction which translates into a reduction of the encapsulationefficiency This was evidenced by Blanco et al when encapsulat-ing BSA and lysozyme in different PLGA-poloxamer microparticles[10] Moreover the type of protein and its initial theoretical loadingare factors directly related with the EE and can affect the colloidalstability of the primary emulsion as shown by Santander et al [12]The different polymersurfactant ratio between the two formula-tions is not comparable since the surfactant is added in a differentway In both cases we used previous formulations as the startingpoint [1022] and tested several polymersurfactant ratios (datanot shown) in order to obtain the best colloidal stability EE andDL In Table 1 we show the data for the optimized PLGAF68 ratiosin both systems
In the W-F68 method despite the higher EE value with respectto O-F68 system an almost complete encapsulation was expecteddue to the low initial proteinPLGA mass ratio [12] and to theabsence of surfactant in the first emulsion step The characteris-tics of the modified formulation process may have the key In thisformulation the relatively high solubility of the ethyl acetate inwater promotes rapid diffusion of the organic solvent into the sec-ond aqueous phase An initial small volume of water containingpoloxamer is initially added to prevent a rapid uncontrolled precip-itation of the polymer and to control the speed of the process Thisis subsequently supplemented with the addition of a larger aque-ous volume as previously described [26] When this solidificationis slow it favors the escape of the protein and the EE decreasesHowever if the solidification is very fast the contact of proteinwith the organic solvent is minimized and the EE increases On thenegative side it can produce polymer agglomeration which inter-feres with the correct formation of the NPs The introduction of anintermediate step with a reduced volume of aqueous phase withpoloxamer can modulate the rate of the process by controlling thediffusion of ethyl acetate into the water and by allowing the diffu-sion into the organic phase of the poloxamer A controlled velocityof the polymer pre-solidification process in the presence of surfac-tant can produce channels or pores in the polymeric shell that onone hand could facilitate the protein release and on the other hand
could drive down the EE value [43] As a result of these phenom-ena the final DLs (ww of lysozymepolymer) shown in Table 1 forboth NP systems are suitable for their application as nanotransportsystems
32 Characterization of the nanoparticles
321 Interfacial characterization of the first water-in- oilemulsion
To gain better insight into the effect of the formulation methodon the interfacial properties of the first water (lysozyme solution)-in-oil emulsion we designed surface experiments with lysozymeand Pluronicreg F68 The main difference in the two formulationmethods is how the Pluronicreg F68 is added in aqueous phase(WndashF68) or in organic phase (O-F68) This difference could affectthe composition of the surface of the NPs and as a result theircolloidal properties
The surface tension and elasticity at the air-water interfacewere the properties analyzed (Table 2) At this interface proteinschange their conformation and expose their hydrophobic part toair depending on their thermodynamic stability flexibility amphi-pathicity molecular size and charge In our case lysozyme is aglobular protein that is adsorbed at the air-water interface andforms a rigid monolayer due to its internal structure and the pres-ence and number of disulfide bridges [44] Our measurements weremade at pH 12 thus lysozyme is negatively charged Table 2 showsthe interfacial tension of the lysozyme monolayer at the air-waterinterface after 50 min of adsorption (457 plusmn 04 (mNm)) and itselasticity (83 plusmn 4 (mNm)) The reduction of the interfacial tensionwhen compared with that of the air-water interface (72 mNm)indicates the surfactant characteristics of the lysozyme The highvalue of elasticity was due to the charge and high molecular inter-actions in the lysozyme monolayer When the monolayer is formedwith Pluronicreg F68 the surface tension is slightly lower than withlysozyme when the Pluronicreg is added in AP but similar (takinginto account the error) when added in OP
Pluronicreg F68 is an amphiphilic molecule that is adsorbed at theair-water interface when it is dissolved in aqueous phase and alsowhen it is deposited onto the surface of the drop Small differencesare found when comparing the surface tension of the Pluronicreg
monolayer from the two methods The different values of interfa-cial tension attained in both cases would be due to the differentmethods to add the Pluronicreg F68 at the formed lysozyme mono-layer Pluronicreg F68 presents lower elasticity than the lysozyme asexpected since Pluronicreg F68 is known to form a flexible monolayerat the air-water interface [45]
Two assays were designed to mimic the formulation methodsof the particles In the first assay (W-F68 method) a monolayer oflysozyme was formed then the bulk of the drop was exchangedwith the aqueous solution of Pluronicreg F68 and after adsorptionthe interfacial tension and elasticity of the interface were mea-sured (379 plusmn 06 mNm and 142 plusmn 05 mNm respectively) Thislow value of elasticity was very similar to that of the monolayerof Pluronicreg F68 indicating that Pluronicreg F68 is located at theinterface and removes the previously adsorbed lysozyme In thesecond assay (O-F68 method) after the monolayer of lysozyme wasformed the Pluronicreg F68 dissolved in chloroform is deposited ontothe surface of the drop The chloroform is rapidly evaporated and
590 I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595
Table 2Interfacial tension and dilatational elasticity (at 1 Hz) of the air-water interface (a) after adsorbing lysozyme or Pluronicreg F68 in the aqueous phase (AP) or Pluronicreg F68 inorganic phase (OP) in the first step (b) when Pluronicreg F68 is added in AP or OP after adsorption of lysozyme monolayer (mean plusmn sd n = 3)
First step Interfacial Tension(mNm)
Elasticitya
(mNm)Second step Interfacial Tension
(mNm)Elasticityb
(mNm)
Lysozyme 457 plusmn 04 83 plusmn 4 Pluronicreg F68 (AP) 379 plusmn 06 142 plusmn 05Lysozyme 457 plusmn 04 83 plusmn 4 Pluronicreg F68 (OP) 38 plusmn 2 43 plusmn 4Pluronicreg F68 (AP) 421 plusmn 03 15 plusmn 3Pluronicreg F68 (OP) 475 plusmn 21 94 plusmn 05
the interfacial tension and elasticity of the interface are measured(38 plusmn 2 mNm and 43 plusmn 4 mNm respectively) The elasticity washalf of that of the pure lysozyme monolayer perhaps because ofthe coexistence of lysozyme and Pluronicreg F68 molecules at theinterface The surface tension of the final interface does not dependon the method of adding the Pluronicreg but it is lower than that ofthe pure lysozyme or the pure Pluronicreg
Within this context it has been widely reported that the adsorp-tion of PEO and poloxamers at the interface reduces the proteinbinding [4647] In the O-F68 method the lysozyme is exposedto the DCM after the formation of the first water-in-oil emulsioneven if Pluronicreg is added as they both coexist at the interface Inthe W-F68 method protein will be in contact with ethyl acetate inthis step as Pluronicreg is absent However this solvent has weakerbiological effects on lysozyme Pluronicreg could reach the interfacewhen added to the aqueous phase in the following step and dis-place the protein from the interface which could diffuse outwardsto the aqueous phase
322 Particle morphologyThe delivery biodistribution and action mechanism of a trans-
ported drug or biomolecule depend heavily on the size of theparticle concentration and timing [48] In general the micromet-ric scale is designed for a local supply that allows the formation ofreservoirs of the transported molecule and minimizes the actionof the phagocytic system [49] However nanometric systems aremore versatile because they permit a systemic distribution aremore stable and reactive and allow extra- as well as intracellu-lar action This latter mechanism is essential when the moleculeor drug should act in the cytoplasm [50] or any other intracellularstructure such as the mitochondria Golgi apparatus endoplasmicreticulum or nucleus [485152] Other parameters to alter the intra-cellular fate of the particles have also been investigated mainly byaltering their surface decoration [53] for example with nuclearlocalization signals (NLS) that use the nucleus as the target of theparticle [51] However these strategies are still in their very earlydevelopmental phase [4852]
A particle size in the submicron scale (between 2 and 500 nm)was sought as it is necessary for cell internalization and a rapiddistribution after parenteral administration in order to reach dif-ferent tissues through different biological barriers Particles under200 nm minimize their intake by macrophages The type of organicsolvent the polymer concentration the addition of surfactant andthe emulsification energy control the size of the system
The O-F68 method gives rise to a monomodal particle-size dis-tribution with diameters around 100 nm The addition of Pluronicreg
F68 in the organic phase bolsters colloidal stability of the first emul-sion and reduces the particle size in comparison with PLGA NPsin which the stability is purely electrostatic due to the carboxylicgroups of the PLGA In the W-F68 method shear stress and volumeof the aqueous phase are taken into account to produce a systemwith particles of between 100 and 500 nm
O-F68-Lys NPs have a spherical shape with a monomodal sizedistribution (diameters around 100 nm) and core-shell structure(Fig 1a) Empty particles produced with the O-F68 method are
shown in Figs S1 (without F68) and S2 (with F68) They are alsospherical and with a core-shell structure but slightly larger
W-F68-Lys NPs also present a spherical shape but a multi-modal size distribution with diameters between 140 and 450 nmthe largest population being around 260 nm (Fig 1b) A core-shellstructure is also observed in these particles Empty particles fromthe W-F68 method are presented in Fig S3 corresponding to a morepolydisperse system
323 Nanoparticle size electrokinetic mobility and colloidalstability
The hydrodynamic diameter distribution of the particles wasdetermined firstly by DLS Table 3 contains the main colloidal prop-erties of particles produced with the O-F68 and W-F68 methodsempty or loaded with lysozyme The results of empty particles fromthe O-F68 method but synthesized without F68 are also included
The size parameters were calculated through a cumulative anal-ysis of the data which is applicable for narrow monomodal sizedistributions [34] SEM and STEM micrographs indicate that suchan approximation could be assumed for particles from the O-F68method but not from the W-F68 one Thus the intensity size distri-butions of the different systems are shown in Fig 2a The presenceof Pluronicreg F68 in the O-F68 method significantly reduces the sizeand polydispersity of the NPs This agrees with the reduction of thesurface tension when the F68 is at the interface (Table 2) whichpromotes the emulsification process If the NPs are also loaded withlysozyme the size is even smaller but the polydispersity increasesslightly compared with the empty particles The surfactant prop-erties of the lysozyme have been shown with the surface-tensionresults (Table 2)
Fig 2a indicates the presence of particles higher than 500 nmwith the W-F68 which does not correlate with the SEM micro-graphs Thus a different technique (NTA) was used to gaininformation on the size distribution of such systems (Fig 3b) WithNTA the size distribution was consistent with the SEM imagesBroad size distributions corresponding to multimodal systemswere found with this method but the addition of lysozyme ledto a clear size reduction This is because lysozyme also acts as anemulsifier in the first emulsion
The electrokinetic charge of the NPs was analyzed by measuringthe electrophoretic mobility For comparison all the samples weremeasured at pH 7 (phosphate buffer) In Fig 3 the electrophoreticmobility distributions are presented while the corresponding -averages are shown in Table 3
PLGA NPs are usually negatively charged due to the carboxylicgroups of the polymer The use of Pluronicreg F68 in the O-F68method clearly reduces the electrophoretic mobility of the NPswhich indicates that some Pluronicreg is located at the NP sur-face This reduction was expected after the incorporation of thisnon-ionic surfactant onto the interface since the presence ofpolyethylene oxide chains would cause an outward shift of theshear plane where the -potential is defined and this would sub-sequently diminish electrophoretic mobility Previous results forPLGA particles have shown a significant reduction directly relatedto the poloxamer coating [54] If we compare the two systems the
I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595 591
Fig 1 SEM and STEM micrographs of lysozyme-loaded particles using O-F68 (a) or W-F68 method (b)
Table 3Colloidal properties of PLGA NPs from different formulation methods They were measured in phosphate buffer (pH 7) The average hydrodynamic diameter (Z-average orcumulative mean) and the polydispersity index (PDI) are determined from DLS (Mean plusmn sd n = 3)
Z-average (nm) PDI -average (mcmVs)
O-F68 method Empty without F68 266 plusmn 7 0293 minus506 plusmn 015Empty 1627 plusmn 21 0081 minus429 plusmn 018Lysozyme-loaded 1210 plusmn 12 0244 minus334 plusmn 007
W-F68 method Empty 273 plusmn 3 0193 minus531 plusmn 011Lysozyme-loaded 293 plusmn 4 0169 minus4212 plusmn 0013
Fig 2 Hydrodynamic diameter distribution (a) by DLS at pH 7 (phosphate buffer) of empty and lysozyme-loaded PLGA particles from the O-F68 and W-F68 methods and(b) by NTA at pH 7 (phosphate buffer) of empty and lysozyme-loaded PLGA particles from the W-F68 method
less negative surface for OF68 NPs would be related to less densityof surface PLGA polymer bringing the negative electrical charge tothe interface This result would be in line with the greater amountof PLGA in the formulation of WF68 nanosystem
When the lysozyme is also used in the synthesis the surfaceis even less negative which could be explained by the presence
of some protein (whose net charge is positive) near or at theinterface This latter effect is also found with the W-F68 methodThe attractive electrostatic interaction between negative terminalacid residues of PLGA and lysozyme molecules plays a key rolein the process of protein encapsulation [41] or adsorption [40]in PLGA NPs which affects the final protein loading In relation
592 I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595
Fig 3 Electrophoretic mobility distribution at pH 7 (phosphate buffer) of empty and lysozyme-loaded PLGA particles from the (a) O-F68 and (b) W-F68 methods
to this situation an important characteristic of the W-F68 encap-sulation formulation is that the water phase is at pH 12 whichallows a negative net charge of lysozyme and thus avoids theelectrostatic protein-polymer attraction This situation can reducethe encapsulation efficiency but at the same time favors the laterprotein-diffusion process and consequently the short-term release
Recent studies have proposed the use of nanoparticles embed-ded in predesigned 3D-printed scaffolds [5556] moving us toanalyze the stability of the two formulations in several mediausually employed during the preparation of other structures Sizedistributions similar to the original were found for the two formula-tions in different media (PB PBS and DMEM) and at different timesafter synthesis (0 1 and 5 days) The electric charge of PLGA acidend groups and the poloxamer molecules located on the NP sur-face confers a combined electrostatic and steric colloidal-stabilitymechanism as has previously been described [4654] Additionallythe NPs in all cases keep their size under storage at 4 C at least for1 month (data not shown) Thus the media described could poten-tially be used as storage media or to prepare other solutions orscaffolds before actually placing them in the living environment(in vitro or in vivo)
324 NMR of the nanoparticlesIn Fig 3 both empty and protein-loaded NPs present less neg-
ative electrophoretic mobility than do empty NPs without F68which could be explained by the presence of Pluronicreg F68 atthe surface of the NP By comparing the 1HNMR spectra of freePluronicreg F68 and lysozyme-loaded NPs from O-F68 and W-F68methods we can check the presence of F68 at the surface of theNPs (Fig S4) by the peaks shown between 325 and 375 ppm and at1 ppm These peaks are also visible in the spectra of NPs formulatedwith F68 (O-F68 and W-F68 Figs S5 and S6 respectively)
33 Biological activity and interactions
A controlled release from a PLGA-based delivery system is adifficult task as it depends on multiple factors the type of PLGAsolvent mechanical stress use of surfactants etc [57] The diffusionof the protein and the polymer erosion are the main mechanismsinvolved in the protein release in PLGA-based delivery systems Fur-thermore it is typical to find a rapid burst release at the initial stagefollowed by a slow release phase over the short and medium termIn this phase protein molecules diffuse through the polymer matrixuntil reaching a final phase in which the polymer degradation byhydrolysis allows a faster release [20]
On the other hand the short-term release is of special inter-est for transporting bone morphogenetic growth factors (BMPs) Acontrolled initial burst followed by a sustained release significantlyimproves in vivo regeneration of bone [3] and cartilage [58] even in
Fig 4 Cumulative release (filled symbols) and residual bioactivity (open symbols)of O-F68-Lys (square) and W-F68-Lys (triangle) incubated for different times at 37 Cin saline phosphate buffer (pH 74) (mean plusmn sd n = 3)
dual-controlled release systems [59] For these reasons we focusedour analysis on short-term release taking into account the reducedpolymer degradation by hydrolysis found for similar systems forthese early steps [60]
Fig 4 shows the accumulative release of lysozyme from O-F68-Lys NPs over the short term (seven days) These results areconsistent with a two-stepped process an initial burst and a slow-release phase The first step could correspond to the release ofthe protein molecules located near surface whose presence wasdeduced from the electrophoretic mobility results (Fig 3) The sec-ond part of the release process was limited and slow due to theprotein diffusion through the matrix of the polymeric shell Thespecific electrostatic interaction between the positive lysozymemolecules and the PLGA negative terminal acid groups can reducethe protein diffusion [10] When the poloxamer (F68) is added theinteraction between the surfactant and the protein helps the diffu-sion process leading to a more complete and sustained release [12]It also helps to keep the biological activity of the protein [4161] Thepoloxamer reduces the non-specific protein-polymer interactions(ie hydrophobic interactions) but not the specific ones (electro-statics) thus the diffusion through water-filled pores or throughthe polymer is still limited In the current study the protein fractionreleased and the release pattern are similar to those found in theliterature for lysozyme encapsulated in nano- and microparticlesof blends of PLGA and other polymers or surfactants [261511]
The protein release curve from W-F68-Lys NPs (Fig 4) revealsthat the initial delivery rate is identical to that of the O-F68 systemwhich could mean a similar proportion of encapsulated proteinclose to or at the surface for both NP systems This would agreewith the analogous decrease in the electrophoretic mobility of the
I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595 593
Fig 5 z-projection of 5 images of hMSCs visualized 30 min after incubation with W-F68-LysFITC NPs or O-F68-LysFITC NPs hMSCs were previously labeled with cell-trackerred Scale bar 20 m
lysozyme-loaded NPs previously reported (Fig 3) In the secondpart of the process the specific interaction between the proteinand the polymer is again present However the diffusion processin the W-F68 system appears to be enhanced allowing a contin-uous and sustained release after the initial burst and reaching aslightly higher value for the maximum release time studied Thisresult could be related to the inner structure of the polymer layerthat allows better hydration and therefore better diffusion of theprotein towards the outside It has been previously reported thatthe use of less polar organic solvents such as DCM for PLGA par-ticles formulations increases the density of the polymer matrix incomparison with more polar organic solvents such as EA The PLGAmatrices prove more resistant in the first case but reducing at thesame time their connectivity and diffusivity [62] Meng et al [26]found that faster removal of EA results in a slower kinetic release ofthe protein due to a decrease in the porosity of the NPs Regardingthe role of the Pluronicreg Rafati et al [63] found a higher concentra-tion of protein encapsulated in the surface pores in microparticlessynthesized in the presence of surfactant in the second aqueousphase of the emulsion Since an intermediate step was introducedin our W-F68 formulation in the second aqueous phase of the emul-sion the removal of the EA by diffusion was strongly controlled sothat it was expected that the porosity of these NPs would increaseThis porosity improves protein diffusion which allows a more sta-ble release pattern according to the experimental result found forthis system Despite the unfavorable effect of the specific electro-static protein-polymer interaction on the release the amount ofreleased protein in our NPs is substantial signifying that there areother unspecific interactions that can be modulated by the pres-ence of surfactant allowing a sustained release The amount ofreleased lysozyme is similar to that found with lysozyme physi-cally adsorbed onto the surface of PLGA nanoparticles despite theelectrostatic attraction [40] Besides other unspecific interactionsthe electrolyte concentration in the release medium could modu-late this electrostatic attraction between the protein and polymerdiminishing it and facilitating the release process [46]
Another remarkable parameter is the biological activity of thein vitro release of lysozyme shown in Fig 4 While in the O-F68system the bioactivity is partially reduced by up to 40 the pro-
tein supplied by the W-F68 system maintains the activity above90 with respect to that of commercially supplied lysozyme andresuspended in the same release buffer As discussed above boththe organic solvent and the hydrophobic interaction between theprotein and the polymer often cause denaturation of encapsulatedproteins [4164] Perez et al [11] describe a partial loss of activ-ity when using DCM and an aqueous PVA solution in the secondemulsification step without any additional excipient The use ofpoloxamers in the formulation reduces such interactions enhancesthe stability of the protein and maintains an aqueous layer thatretains the water molecules necessary for the biological functionof the protein at the same time aiding its diffusion This situationtogether with the use of a weak organic solvent such as EA helpspreserve the biological activity of the lysozyme as found for theW-F68-Lys system
Fig S7 presents different confocal microscopy images relatedto the release process of lysozyme-loaded W-F68 NPs A decreasein fluorescence intensity was appreciable over the course of thein vitro experiment In addition the aggregation of the system isvisible as the incubation process progresses The analysis of theseimages is consistent with the previously reported results for thisNP system
331 Cellular uptakeCellular uptake of PLGA NPs is a known process affected
mainly by surface properties and functionalization [9] and parti-cle aggregation [65] Internalization and subsequent intracellularprocessing of the particles have been described as an activeprocess thus it is energy dependent and can therefore beaffected by other factors that alter the energy uptake by cellssuch as temperature [48] Particles can be internalized by sev-eral endocytosis methods dependent primarily on the size ofthe particle caveolin-dependent particles (diameter asymp 60 nm)clathrin-independent (diameter asymp 90 nm) and clathrin-dependent(diameter asymp 120 nm) [5152] Once internalized about 65 areexported back to the extracellular space before releasing anyof their content while the rest slowly release the encapsulatedmolecule into the intracellular space [66] The intracellular releaseprocess is affected by the formulation of the particles [48] We have
594 I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595
demonstrated that the proposed systems follow a pattern similar toothers previously published As early as 30 min after incubation W-F68-LysFITC NPs were taken up by the cells (Fig 5) Some W-F68particles were still in the medium so that the dual activity couldhappen In contrast O-F68-LysFITC NPs were affected by aggrega-tion and therefore did not properly reach the intracellular space(Fig 5 for z-axis images view Fig S8) This contradicts the previousanalyses of the colloidal stability in PB PBS and DMEM This findingcan be explained by the fact that although the culture media wasDMEM this latter medium was supplemented with fetal bovineserum and cells release many factors to the extracellular mediumthat can affect these types of particles None of the systems wereshown to be toxic for the cells (Fig S9) No studies available havereported any effects of lysozyme on hMSCs
4 Conclusions
A novel dual-delivery PLGA-nanosystem has been developedin which the formulation and components favor an adequateshort-term delivery pattern while preserving the bioactivity ofencapsulated molecules The analysis of the polymer-surfactant-protein interaction shows that the organic solvent use ofsurfactant volume relation of both phases and the net charge of theprotein play important roles in the final characteristics and releasebehavior of the nanoparticles The W-F68 formulation balances allof them in order to provide a nanosystem ready to transport anddeliver hydrophilic biomolecules such as proteins In vitro releaseexperiments display an adequate short-term delivery pattern thatat the same time preserves the bioactivity of the encapsulatedbiomolecule Additionally the singular nanoparticle size distribu-tion found for this W-F68 nanosystem allows the possibility of adual outer- and intra-cellular protein delivery as has been shownby in vitro cellular experiments This novel formulation will be usedin future studies to encapsulate and deliver growth factors in vitroand in vivo in order to exploit the therapeutic potential of thisnanosystem
Acknowledgements
The authors wish to express their appreciation for the tech-nical support to Dr Azahara Rata-Aguilar and for the financialsupport granted by the Consejeriacutea de Economiacutea Innovacioacuten Cienciay Empleo de la Junta de Andaluciacutea (Spain) through research groupsFQM-115 and CTS-1028 and by the following research projectMAT2013-43922-R ndash European FEDER support included minus (MICINNSpain)
Appendix A Supplementary data
Supplementary data associated with this article can be found inthe online version at httpdxdoiorg101016jcolsurfb201708027
References
[1] M Padial-Molina JT Marchesan AD Taut Q Jin WV Giannobile HF RiosMethods to Validate Tooth-supporting Regenerative Therapies 2012 httpdxdoiorg101007978-1-61779-860-3 13
[2] M Padial-Molina JC Rodriguez SL Volk HF Rios Standardized in vivomodel for studying novel regenerative approaches for multitissuebone-ligament interfaces Nat Protoc 10 (2015) httpdxdoiorg101038nprot2015063
[3] Bone regeneration from PLGA micro-Nanoparticles Biomed Res Int (2015)httpwwwhindawicomjournalsbmriaa415289
[4] K-B Lee A Solanki J Kim J Jung Nanomedicine dynamic integration ofnanotechnology with biomedical science in Handb Clin Nanomedicine PanStanford 2016 2017 pp 21ndash60 httpdxdoiorg101201b19915-4
[5] GEJ Poinern A Laboratory Course in Nanoscience and Nanotechnology CRCPress Taylor amp Francis Group 2015
[6] MM Yallapu BK Gupta M Jaggi SC Chauhan Fabrication of curcuminencapsulated PLGA nanoparticles for improved therapeutic effects inmetastatic cancer cells J Colloid Interface Sci 351 (2010) 19ndash29 httpdxdoiorg101016jjcis201005022
[7] BP Nair CP Sharma Poly(lactide-co-glycolide)-laponite-F68 nanocompositevesicles through a single-step double-emulsion method for the controlledrelease of doxorubicin Langmuir 28 (2012) 4559ndash4564 httpdxdoiorg101021la300005c
[8] R Shankarayan S Kumar P Mishra Differential permeation ofpiroxicam-loaded PLGA micronanoparticles and their in vitro enhancementJ Nanopart Res 15 (2013) 1496 httpdxdoiorg101007s11051-013-1496-6
[9] JA Loureiro B Gomes G Fricker MAN Coelho S Rocha MC PereiraCellular uptake of PLGA nanoparticles targeted with anti-amyloid andanti-transferrin receptor antibodies for Alzheimerrsquos disease treatmentColloids Surf B Biointerfaces 145 (2016) 8ndash13 httpdxdoiorg101016jcolsurfb201604041
[10] D Blanco MJ Alonso Protein encapsulation and release frompoly(lactide-co-glycolide) microspheres effect of the protein and polymerproperties and of the co- encapsulation of surfactants Eur J PharmBiopharm 45 (1998) 285ndash294 httpdxdoiorg101016S0939-6411(98)00011-3
[11] C Peacuterez P De Jesuacutes K Griebenow Preservation of lysozyme structure andfunction upon encapsulation and release from poly(lactic-co-glycolic) acidmicrospheres prepared by the water-in-oil-in-water method Int J Pharm248 (2002) 193ndash206 httpdxdoiorg101016S0378-5173(02)00435-0
[12] MJ Santander-Ortega N Csaba L Gonzaacutelez D Bastos-Gonzaacutelez JLOrtega-Vinuesa MJ Alonso Protein-loaded PLGAndashPEO blend nanoparticlesencapsulation release and degradation characteristics Colloid Polym Sci288 (2010) 141ndash150 httpdxdoiorg101007s00396-009-2131-z
[13] N Pirooznia S Hasannia A Lotfi M Ghanei Encapsulation of alpha-1antitrypsin in PLGA nanoparticles in Vitro characterization as an effectiveaerosol formulation in pulmonary diseases J Nanobiotechnol 10 (2012) 20httpdxdoiorg1011861477-3155-10-20
[14] I DrsquoAngelo M Garcia-Fuentes Y Parajoacute A Welle T Vaacutentus A Horvaacuteth GBoumlkoumlnyi G Keacuteri MJ Alonso Nanoparticles based on PLGA poloxamer blendsfor the delivery of proangiogenic growth factors Mol Pharm 7 (2010)1724ndash1733 httpdxdoiorg101021mp1001262
[15] LJ White GTS Kirby HC Cox R Qodratnama O Qutachi FRAJ Rose KMShakesheff Accelerating protein release from microparticles for regenerativemedicine applications Mater Sci Eng C 23 (2013) 2578ndash2583 httpdxdoiorg101016jmsec201302020
[16] P Pantazis K Dimas JH Wyche S Anant CW Houchen J Panyam RPRamanujam Preparation of siRNA-Encapsulated PLGA nanoparticles forsustained release of siRNA and evaluation of encapsulation efficiencyNanopart Biol Med (2012) 311ndash319 httpdxdoiorg101007978-1-61779-953-2 25 Humana Press Totowa NJ
[17] JS Park HN Yang DG Woo SY Jeon KH Park Multilineage differentiationof human-derived dermal fibroblasts transfected with genes coated on PLGAnanoparticles plus growth factors Biomaterials 34 (2013) 582ndash597 httpdxdoiorg101016jbiomaterials201210001
[18] F Wan M Yang Design of PLGA-based depot delivery systems forbiopharmaceuticals prepared by spray drying Int J Pharm 498 (2016)82ndash95 httpdxdoiorg101016jijpharm201512025
[19] A Giteau MC Venier-Julienne A Aubert-Poueumlssel JP Benoit How to achievesustained and complete protein release from PLGA-based microparticles IntJ Pharm 350 (2008) 14ndash26 httpdxdoiorg101016jijpharm200711012
[20] S Fredenberg M Wahlgren M Reslow A Axelsson The mechanisms of drugrelease in poly(lactic-co-glycolic acid)-based drug delivery systemsmdashareview Int J Pharm 415 (2011) 34ndash52 httpdxdoiorg101016jijpharm201105049
[21] F Mohamed CF van der Walle Engineering biodegradable polyesterparticles with specific drug targeting and drug release properties J PharmSci 97 (2008) 71ndash87 httpdxdoiorg101002jps21082
[22] N Csaba L Gonzaacutelez A Saacutenchez MJ Alonso Design and characterisation ofnew nanoparticulate polymer blends for drug delivery J Biomater Sci PolymEd 15 (2004) 1137ndash1151 httpdxdoiorg1011631568562041753098
[23] HK Makadia SJ Siegel Poly lactic-co-glycolic acid (PLGA) as biodegradablecontrolled drug delivery carrier Polymers (Basel) 3 (2011) 1377ndash1397 httpdxdoiorg103390polym3031377
[24] F Danhier E Ansorena JM Silva R Coco A Le Breton V Preacuteat PLGA-basednanoparticles an overview of biomedical applications J Controlled Release161 (2012) 505ndash522 httpdxdoiorg101016jjconrel201201043
[25] G Ratzinger U Laumlnger L Neutsch F Pittner M Wirth F Gabor Surfacemodification of PLGA particles the interplay between stabilizer ligand sizeand hydrophobic interactions Langmuir 26 (2010) 1855ndash1859 httpdxdoiorg101021la902602z
[26] FT Meng GH Ma W Qiu ZG Su WOW double emulsion technique usingethyl acetate as organic solvent effects of its diffusion rate on thecharacteristics of microparticles J Controlled Release 91 (2003) 407ndash416httpdxdoiorg101016S0168-3659(03)00273-6
[27] M Padial-Molina F OrsquoValle A Lanis F Mesa DM Dohan Ehrenfest H-LWang P Galindo-Moreno Clinical application of mesenchymal stem cells andnovel supportive therapies for oral bone regeneration Biomed Res Int 2015(2015) httpdxdoiorg1011552015341327
I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595 595
[28] P Yilgor N Hasirci V Hasirci Sequential BMP-2BMP-7 delivery frompolyester nanocapsules J Biomed Mater Res ndash Part A 93 (2010) 528ndash536httpdxdoiorg101002jbma32520
[29] B Li T Yoshii AE Hafeman JS Nyman JC Wenke SA Guelcher The effectsof rhBMP-2 released from biodegradable polyurethanemicrospherecomposite scaffolds on new bone formation in rat femora Biomaterials 30(2009) 6768ndash6779 httpdxdoiorg101016jbiomaterials200908038
[30] Y Wang Y Wei X Zhang M Xu F Liu Q Ma Q Cai X Deng PLGAPDLLAcore-shell submicron spheres sequential release system preparationcharacterization and promotion of bone regeneration in vitro and in vivoChem Eng J 273 (2015) 490ndash501 httpdxdoiorg101016jcej201503068
[31] YB Shim HH Jung JW Jang HS Yang H Bae JC Park B Choi SH LeeFabrication of hollow porous PLGA microspheres using sucrose for controlleddual delivery of dexamethasone and BMP2 J Ind Eng Chem 37 (2016)101ndash106 httpdxdoiorg101016jjiec201603014
[32] J Maldonado-Valderrama JAH Terriza A Torcello-Goacutemez MACabrerizo-Viacutelchez In vitro digestion of interfacial protein structures SoftMatter (2013) 1043ndash1053 httpdxdoiorg101039c2sm26843d
[33] M a Cabrerizo-Vilchez H a Wege J a Holgado-Terriza a W NeumannAxisymmetric drop shape analysis as penetration Langmuir balance Rev SciInstrum 70 (1999) 2438ndash2444 httpdxdoiorg10106311149773
[34] PA Hassan S Rana G Verma Making sense of brownian motion colloidcharacterization by dynamic light scattering Langmuir 31 (2015) 3ndash12httpdxdoiorg101021la501789z
[35] RJ Kok M Haas F Moolenaar D de Zeeuw DK Meijer Drug delivery to thekidneys and the bladder with the low molecular weight protein lysozymeRen Fail 20 (1998) 211ndash217
[36] S Mason SA Tarle W Osibin Y Kinfu D Kaigler Standardization and safetyof alveolar bone-derived stem cell isolation J Dent Res 93 (2014) 55ndash61httpdxdoiorg1011770022034513510530
[37] C Farace P Saacutenchez-Moreno M Orecchioni R Manetti F Sgarrella Y AsaraJM Peula-Garciacutea JA Marchal R Madeddu LG Delogu Immune cell impactof three differently coated lipid nanocapsules pluronic chitosan andpolyethylene glycol Sci Rep 6 (2016) 18423 httpdxdoiorg101038srep18423
[38] C Sturesson J Carlfors Incorporation of protein in PLG-microspheres withretention of bioactivity J Controlled Release 67 (2000) 171ndash178 httpdxdoiorg101016S0168-3659(00)00205-4
[39] L Ying S Jiali J Guoqiang Z Jia D Fuxin In vitro evaluation oflysozyme-loaded microspheres in thermosen- sitive methylcellulose-basedhydrogel Chin J Chem Eng 15 (2007) 566ndash572
[40] C Cai U Bakowsky E Rytting AK Schaper T Kissel Charged nanoparticlesas protein delivery systems a feasibility study using lysozyme as modelprotein Eur J Pharm Biopharm 69 (2008) 31ndash42 httpdxdoiorg101016jejpb200710005
[41] A Paillard-Giteau VT Tran O Thomas X Garric J Coudane S Marchal IChourpa JP Benoicirct CN Montero-Menei MC Venier-Julienne Effect ofvarious additives and polymers on lysozyme release from PLGA microspheresprepared by an sow emulsion technique Eur J Pharm Biopharm 75 (2010)128ndash136 httpdxdoiorg101016jejpb201003005
[42] MJ Santander-Ortega D Bastos-Gonzaacutelez JL Ortega-Vinuesa MJ AlonsoInsulin-loaded PLGA nanoparticles for oral administration an in vitrophysico-chemical characterization J Biomed Nanotechnol 5 (2009) 45ndash53httpdxdoiorg101166jbn2009022
[43] ID Rosca F Watari M Uo Microparticle formation and its mechanism insingle and double emulsion solvent evaporation J Controlled Release 99(2004) 271ndash280 httpdxdoiorg101016jjconrel200407007
[44] S Pezennec F Gauthier C Alonso F Graner T Croguennec G Bruleacute ARenault The protein net electric charge determines the surface rheologicalproperties of ovalbumin adsorbed at the air-water interface FoodHydrocolloids 14 (2000) 463ndash472 httpdxdoiorg101016S0268-005X(00)00026-6
[45] A Torcello-Goacutemez MJ Santander-Ortega JM Peula-Garciacutea JMaldonado-Valderrama MJ Gaacutelvez-Ruiz JL Ortega-Vinuesa AMartiacuten-Rodriacuteguez Adsorption of antibody onto pluronic F68-coverednanoparticles link with surface properties Soft Matter 7 (2011) 8450 httpdxdoiorg101039c1sm05570d
[46] MJ Santander-Ortega MV Lozano-Loacutepez D Bastos-Gonzaacutelez JMPeula-Garciacutea JL Ortega-Vinuesa Novel core-shell lipid-chitosan andlipid-poloxamer nanocapsules stability by hydration forces Colloid PolymSci 288 (2010) 159ndash172 httpdxdoiorg101007s00396-009-2132-y
[47] A Torcello-Goacutemez MJ Santander-Ortega JM Peula-Garciacutea JMaldonado-Valderrama MJ Gaacutelvez-Ruiz JL Ortega-Vinuesa AMartiacuten-Rodriacuteguez Adsorption of antibody onto Pluronic F68-coverednanoparticles link with surface properties Soft Matter 7 (2011) httpdxdoiorg101039c1sm05570d
[48] JP Penaloza V Maacuterquez-Miranda M Cabana-Brunod R Reyes-Ramiacuterez FMLlancalahuen C Vilos F Maldonado-Biermann LA Velaacutesquez JA FuentesFD Gonzaacutelez-Nilo M Rodriacuteguez-Diacuteaz C Otero Intracellular trafficking andcellular uptake mechanism of PHBV nanoparticles for targeted delivery inepithelial cell lines J Nanobiotechnol 15 (2017) 1 httpdxdoiorg101186s12951-016-0241-6
[49] SP Schwendeman RB Shah BA Bailey AS Schwendeman Injectablecontrolled release depots for large molecules J Controlled Release 190 (2014)240ndash253 httpdxdoiorg101016jjconrel201405057
[50] H Wang SCG Leeuwenburgh Y Li JA Jansen The use of micro- andnanospheres as functional components for bone tissue regeneration TissueEng Part B Rev 18 (2012) 24ndash39 httpdxdoiorg101089tenteb20110184
[51] JK Vasir V Labhasetwar Biodegradable nanoparticles for cytosolic deliveryof therapeutics Adv Drug Deliv Rev 59 (2007) 718ndash728 httpdxdoiorg101016jaddr200706003
[52] B Yameen W Il Choi C Vilos A Swami J Shi OC Farokhzad Insight intonanoparticle cellular uptake and intracellular targeting J Controlled Release190 (2014) 485ndash499 httpdxdoiorg101016jjconrel201406038
[53] H Sneh-Edri D Likhtenshtein D Stepensky Intracellular targeting of PLGAnanoparticles encapsulating antigenic peptide to the endoplasmic reticulumof dendritic cells and its effect on antigen cross-presentation in vitro MolPharm 8 (2011) 1266ndash1275 httpdxdoiorg101021mp200198c
[54] MJ Santander-Ortega AB Joacutedar-Reyes N Csaba D Bastos-Gonzaacutelez JLOrtega-Vinuesa Colloidal stability of Pluronic F68-coated PLGAnanoparticles a variety of stabilisation mechanisms J Colloid Interface Sci302 (2006) 522ndash529 httpdxdoiorg101016jjcis200607031
[55] B Baumann T Jungst S Stichler S Feineis O Wiltschka M Kuhlmann MLindn J Groll Control of nanoparticle release kinetics from 3D printedhydrogel scaffolds Angew Chem ndash Int Ed 56 (2017) 4623ndash4628 httpdxdoiorg101002anie201700153
[56] S-J Lee W Zhu L Heyburn M Nowicki B Harris LG Zhang Developmentof novel 3-D printed scaffolds with core-shell nanoparticles for nerveregeneration IEEE Trans Biomed Eng 64 (2017) 408ndash418 httpdxdoiorg101109tbme20162558493
[57] DJ Hines DL Kaplan Poly(lactic-co-glycolic) acid-controlled-releasesystems experimental and modeling insights Crit Rev Ther Drug CarrierSyst 30 (2013) 257ndash276 httpdxdoiorg101615CritRevTherDrugCarrierSyst2013006475
[58] H Begam SK Nandi B Kundu A Chanda Strategies for delivering bonemorphogenetic protein for bone healing Mater Sci Eng C 70 (2016)856ndash869 httpdxdoiorg101016jmsec201609074
[59] YH Kim Y Tabata Dual-controlled release system of drugs for boneregeneration Adv Drug Deliv Rev 94 (2015) 28ndash40 httpdxdoiorg101016jaddr201506003
[60] N Rescignano L Tarpani A Romani I Bicchi S Mattioli C Emiliani L TorreJM Kenny S Martino L Latterini I Armentano In-vitro degradation of PLGAnanoparticles in aqueous medium and in stem cell cultures by monitoring thecargo fluorescence spectrum Polym Degrad Stab 134 (2016) 296ndash304httpdxdoiorg101016jpolymdegradstab201610017
[61] M Morille T Van-Thanh X Garric J Cayon J Coudane D Noeumll MCVenier-Julienne CN Montero-Menei New PLGA-P188-PLGA matrix enhancesTGF-3 release from pharmacologically active microcarriers and promoteschondrogenesis of mesenchymal stem cells J Control Release 170 (2013)99ndash110 httpdxdoiorg101016jjconrel201304017
[62] A Bohr F Wan J Kristensen M Dyas E Stride S Baldursdottiacuter MEdirisinghe M Yang Pharmaceutical microparticle engineering withelectrospraying the role of mixed solvent systems in particle formation andcharacteristics J Mater Sci Mater Med 26 (2015) 61 httpdxdoiorg101007s10856-015-5379-5
[63] A Rafati A Boussahel KM Shakesheff AG Shard CJ Roberts X Chen DJScurr S Rigby-Singleton P Whiteside MR Alexander MC Davies Chemicaland spatial analysis of protein loaded PLGA microspheres for drug deliveryapplications J Control Release 162 (2012) 321ndash329 httpdxdoiorg101016jjconrel201205008
[64] R Gaudana M Gokulgandhi V Khurana D Kwatra AK Mitra Design andevaluation of a novel nanoparticulate-based formulation encapsulating a HIPcomplex of lysozyme Pharm Dev Technol 18 (2013) 752ndash759 httpdxdoiorg103109108374502012737806
[65] S Xiong X Zhao BC Heng KW Ng JSC Loo Cellular uptake of Poly-(DL-lactide-co-glycolide) (PLGA) nanoparticles synthesized through solventemulsion evaporation and nanoprecipitation method Biotechnol J 6 (2011)501ndash508 httpdxdoiorg101002biot201000351
[66] J Panyam V Labhasetwar Dynamics of endocytosis and exocytosis of poly (DL-lactide-co-glycolide) nanoparticles in vascular smooth muscle cells PharmRes 20 (2003) 212ndash220 httpwwwncbinlmnihgovpubmed12636159
pharmaceutics
Article
Formulation Colloidal Characterization and In VitroBiological Ecrarrect of BMP-2 Loaded PLGANanoparticles for Bone Regeneration
Teresa del Castillo-Santaella 1 Inmaculada Ortega-Oller 2 Miguel Padial-Molina 2 Francisco OrsquoValle 3 Pablo Galindo-Moreno 2 Ana Beleacuten Joacutedar-Reyes 14 andJoseacute Manuel Peula-Garciacutea 15
1 Biocolloid and Fluid Physics Group Department of Applied Physics University of Granada 18071 GranadaSpain
2 Department of Oral Surgery and Implant Dentistry University of Granada 18071 Granada Spain3 Department of Pathology School of Medicine amp IBIMER University of Granada 18071 Granada Spain4 Excellence Research Unit ldquoModeling Naturerdquo (MNat) University of Granada 18071 Granada Spain5 Department of Applied Physics II University of Malaga 29071 Malaga Spain Correspondence jmpeulaumaes Tel +34-952132722
Received 20 June 2019 Accepted 31 July 2019 Published 3 August 2019$amp($amp
Abstract Nanoparticles (NPs) based on the polymer poly (lactide-co-glycolide) acid (PLGA) havebeen widely studied in developing delivery systems for drugs and therapeutic biomolecules due tothe biocompatible and biodegradable properties of the PLGA In this work a synthesis method forbone morphogenetic protein (BMP-2)-loaded PLGA NPs was developed and optimized in order tocarry out and control the release of BMP-2 based on the double-emulsion (wateroilwater WOW)solvent evaporation technique The polymeric surfactant Pluronic F68 was used in the synthesisprocedure as it is known to have an ecrarrect on the reduction of the size of the NPs the enhancement oftheir stability and the protection of the encapsulated biomolecule Spherical solid polymeric NPswere synthesized showing a reproducible multimodal size distribution with diameters between100 and 500 nm This size range appears to allow the protein to act on the cell surface and at thecytoplasm level The ecrarrect of carrying BMP-2 co-adsorbed with bovine serum albumin on the NPsurface was analyzed The colloidal properties of these systems (morphology by SEM hydrodynamicsize electrophoretic mobility temporal stability protein encapsulation and short-term release profile)were studied The ecrarrect of both BMP2-loaded NPs on the proliferation migration and osteogenicdicrarrerentiation of mesenchymal stromal cells from human alveolar bone (ABSC) was also analyzedin vitro
Keywords BMP-2 PLGA nanoparticles Pluronic F68
1 Introduction
In the context of nanomedicine tissue regeneration using colloidal micro- and nano-structureshaving unique size and surface activity has received increasing attention over recent years Many ecrarrortshave been made to improve the engineering of these nano-systems in order to reach a ldquosmartrdquo deliveryof bioactive molecules in order to optimize their therapeutic advantages and minimize harmful sideecrarrects [1] With this aim a broad spectrum of biocompatible nanocarriers has been described showingproperties suitable for dicrarrerent biological and therapeutic applications [2] Among these variedproposals polymeric nanosystems represent a major group in which poly lactic-co-glycolic acid (PLGA)is one of the most widely used due to its biocompatibility biodegradability and low cytotoxicitygaining the approval from dicrarrerent drug agencies for human use [34]
Pharmaceutics 2019 11 388 doi103390pharmaceutics11080388 wwwmdpicomjournalpharmaceutics
Pharmaceutics 2019 11 388 2 of 18
PLGA-based structures are described as micro- and nanocarriers to deliver a wide variety of activemolecules and drugs synthetic or natural molecules with hydrophilic or hydrophobic properties andbiomolecules from proteins to nucleic acids [5ndash7] PLGA micro- and nanosystems can be set up usingdicrarrerent formulation techniques with the possibility of a systemic or local distribution These systemscan be applied not only in tissue regeneration but also in very diverse therapies Anticancer drugdelivery infections inflammatory diseases or gene therapy [3] Despite this great potential certainapplications especially in protein encapsulation are hindered by problems such as an uncontrolledrelease profile and protein denaturation [8ndash11]
The water-in oil-in water (WOW) double emulsion method is an ldquoemulsion solvent evaporationrdquotechnique frequently used to encapsulate hydrophilic molecules as proteins in PLGA NPs [612]The appropriate choice of organic solvents the use of polymer-surfactant blends and the addition ofstabilizer-protective agents have proved to be key aspects for optimizing the resulting systems [911]Additionally a surface specific functionalization can be used to improve their versatility allowingthe chemical surface immobilization of dicrarrerent molecules in order to confer targeting or adhesiveproperties to these nanocarriers [13]
Within tissue engineering bone regeneration has a broad range of applications mostly in the fieldof dentistry where PLGA is suggested as a reference polymer to formulate NPs with bone-healinguses [14] The literature describes the delivery of bioactive molecules normally growth factors usingpolymeric microparticles (MPs) and NPs with PLGA as the main component [13] Among the bonemorphogenetic growth factors BMP-2 (bone morphogenetic protein 2) has been the most frequentlycited with many examples in which encapsulation or surface adsorption enables adequate entrapmenteciency and diverse release patterns [15ndash19] For proteins with a very short half-life such as BMPsbiodegradable PLGA nanosystems provide protection and optimal dosage for an adequate stimulationof cell dicrarrerentiation [2021]
Thus within this scenario in the present work we seek to optimize a nano-particulate system inorder to carry out and control the release of BMP-2 using as a starting point the synthesis procedure ofa lysozyme-loaded NP system previously described for the encapsulation of that model protein [11]Also to encapsulate BMP-2 we prepared a second system in which this protein was co-adsorbedwith bovine serum albumin onto the surface of empty NPs The size and morphology the proteinencapsulation eciency the surface characteristics and the colloidal and temporal stability werestudied to complete the physico-chemical characterization of both NP systems
The release profile of BMP-2 indicates the potential of a PLGA nanocarrier for bone regenerationand depends heavily on the polymer degradation by hydrolysis [22] However over the shortterm during which the release does not depend on this chemical degradation proper control ofrelease is necessary in order to modulate other physical processes Thus we focused our releaseexperiments on the short-term using dicrarrerent techniques to compare the two NP samples and establishthe corresponding BMP-2 release profiles Finally the biological activity (cell migration proliferationand osteogenic dicrarrerentiation) was tested in vitro using mesenchymal stromal cells (MSCs) derivedfrom alveolar bone [23]
2 Materials and Methods
21 Nanoparticle Synthesis
211 Formulation
Poly(lactide-co-glycolide) acid (PLGA 5050) ([C2H2O2]x[C3H4O2]y) x = 50 y = 50 (Resomerreg
503H (Evonik Essen Germany) 32ndash44 kDa was used as the polymer and polymeric surfactantPluronic F68 (Poloxamer 188) (Sigma-Aldrich St Louis MO USA) as the emulsifier Their structurebased on a poly(ethylene oxide)-block-poly(propylene oxide)-block-poly(ethylene oxide) is expressedas PEOa-PPOb-PEOa with a = 75 and b = 30 Human recombinant bone morphogenetic proteinrhBMP-2 (Sigma-H4791) was used as therapeutic biomolecule Water was purified in a Milli-Q
Pharmaceutics 2019 11 388 3 of 18
Academic Millipore system A double-emulsion synthesis method was used following a procedurepreviously described with slight modifications [11] In this method 100 mg of PLGA and 3 mg ofdeoxycholic acid (DC) were dissolved in a tube containing 1 mL of ethyl acetate (EA) and vortexedIn total 40 microL of a bucrarrered solution at pH 128 with or without rhBMP-2 (200 microgmL) were addedand immediately sonicated (Branson Ultrasonics 450 Analog Sonifier) for 1 min (Duty cycle dial 20Output control dial 4) with the tube surrounded by ice This primary WO emulsion was poured intoa plastic tube containing 2 mL of a bucrarrered solution (pH 12) of F68 at 1 mgmL and vortexing for30 s Then the tube surrounded by ice was sonicated at the maximum amplitude for the micro tip for1 min (Output control 7) This second WOW emulsion was poured into a glass containing 10 mL ofthe bucrarrered F68 solution and kept under magnetic stirring for 2 min The organic solvent was thenrapidly extracted by evaporation under vacuum to a final volume of 8 mL The resulting empty andBMP-2 encapsulated NP systems were named NP and NP-BMP2 respectively A detailed scheme ofthe synthesis procedure with a yield based on the PLGA component always higher than 85 is shownin Figure S1 of the Supplementary Materials
212 Cleaning and Storage
After the organic solvent evaporation the sample was centrifuged for 10 min at 20 C at 12000 rpmThe supernatant was filtered using Millipore nanofilters 01microm for measuring the free non-encapsulatedprotein The pellet was then resuspended in phosphate bucrarrer (115 mM NaH2PO4) PB to a finalvolume of 4 mL and kept refrigerated at 4 C Under these conditions the systems kept colloidalstability at least for one month
213 Protein Loading and Encapsulation Eciency
The initial protein loading was optimized for the nanoparticle formulation preserving the finalcolloidal stability after the evaporation step and taking into account the amounts shown in the literaturefor this growth factor when encapsulated inside PLGA NPs [2425] Thus we chose 2 microg as the initialtotal mass of rhBMP-2 which means a relation of 2 105 ww (rhBMP-2PLGA) The amount ofencapsulated rhBMP-2 was calculated by measuring the dicrarrerence between the initial added amountand the free non-encapsulated protein present in the supernatant after the cleaning step which wastested by a specific enzyme-linked immuno-sorbent assay following the instructions of the manufacturer(ELISA kit RAB0028 from Sigma-Aldrich St Louis MO USA) Then protein-encapsulation eciency(EE) was calculated as follows
EE =MI MF
MI 100
where MI is the initial total mass of rhBMP-2 and MF is the total mass of rhBMP-2 in theaqueous supernatant
214 Physical Protein Adsorption
Bovine serum albumin (BSA) and rhBMP-2 were coupled on the empty nanoparticle surface bya physical adsorption method The appropriate volume of an aqueous protein solution containing05 mg of BSA and 2 microg of rhBMP-2 was mixed with 5 mL of acetate bucrarrer (pH 5) containing emptyNPs with 125 mg of PLGA This provided a starting amount of proteins corresponding to 004 ww(proteinPLGA) while the mass relation between proteins was 04 ww (rhBMP-2BSA) This solutionwas incubated at room temperature for 2 h under mechanical stirring The nanoparticles were separatedfrom the bucrarrer solution by centrifugation and after the supernatants were filtered (Millipore nanofilters01 microm) they were qualitatively analyzed by gel electrophoresis while the protein quantification wasmade by a bicinchoninic acid protein assay (BCA) (Sigma-Aldrich St Louis MO USA) for BSA andthe specific ELISA for rhBMP-2 The nanoparticle pellet was resuspended in phosphate bucrarrer (pH 74)and stored at 4 C This system was named NP-BSA-BMP2
Pharmaceutics 2019 11 388 4 of 18
215 Protein Separation by Gel Electrophoresis SDS-PAGE
The protein-loaded NPs and dicrarrerent supernatants were treated at 90 C for 10 min in thefollowing bucrarrer 625 mM Tris-HCl (pH 68 at 25 C) 2 (wv) sodium dodecyl sulfate (SDS) 10glycerol 001 (wv) bromophenol blue 40 mM dithiothreitol (DTT) Samples were then separated bysize in porous 12 polyacrylamide gel (1D SDS polyacrylamide gel electrophoresis) under the ecrarrectof an electric field The electrophoresis was run under constant voltage (130 V 45 min) and the gelswere stained using a Coomassie Blue solution (01 Coomassie Brilliant Blue R-250 50 methanol and10 glacial acetic acid) and destained with the same solution lacking the dye
22 Nanoparticle Characterization Morphology Size Concentration and Electrokinetic Mobility
NPs were imaged by scanning electron microscopy (SEM) with a Zeiss SUPRA 40VP field-emissionscanning electron microscope from the Scientific Instrumentation Center of the University of Granada(CIC UGR)
The hydrodynamic size distribution of the NPs was evaluated by nanoparticle tracking analysis(NTA) with a NanoSight LM10-HS (GB) FT14 (NanoSight Amesbury UK) and an sCMOS cameraThe particle concentration according to the diameter (size distribution) was calculated as an averageof at least three independent size distributions The total concentration of NPs of each system wasdetermined in order to control the number of particles used in cell experiments The measurementconditions for all samples were 25 C a viscosity of 089 cP a measurement time of 60 s and a cameragain of 250 The camera shutter was 11 and 15 ms for the empty and BMP-loaded NPs respectivelyThe detection threshold was fixed at 5
The electrophoretic mobility of the NPs was determined using a Zetasizerreg NanoZeta ZS device(Malvern Instrument Ltd Malvern UK) working at 25 C with an He-Ne laser of 633 nm and a 173
scattering angle Each data point was taken as an average over three independent sample measurementsFor each sample the electrophoretic mobility distribution and the average electrophoretic mobility(micro-average) were determined by the technique of laser Doppler electrophoresis
23 Colloidal and Temporal Stability in Biological Media
The average hydrodynamic diameter and the polydispersity index (PDI) by dynamic lightscattering (DLS) of each NP system were measured in dicrarrerent media (phosphate bucrarrer (PB) salinephosphate bucrarrer (PBS) and cell culture medium Dulbeccorsquos modified Eaglersquos medium DMEM(Sigma)) Also data on temporal stability were gathered by repeating these analyses at dicrarrerent timesafter synthesis (0 1 and 5 days) and after 1 month under storage conditions
In vitro release experiments were conducted as follows 1 mL of each sample for each incubationtime was suspended in PBS at 37 C After the corresponding time (24 48 96 168 h) NPs wereseparated from the supernatant of released proteins by centrifugation for 10 min at 14000 rpm (10 C)The NP pellet was suspended in 1 mL of 005 M NaOH and stirred for 2 h for a complete polymerdegradation The alkaline protein solution was assayed by BCA and ELISA to quantify the unreleasedamount The protein released was calculated taking into account the total encapsulated amount Allexperiments were made in triplicate
24 Cell Interactions
For all biological in vitro studies a cell population cultured from the maxillary alveolar bonewas used This population was previously characterized and confirmed to present all characteristicsof a mesenchymal stromal cell population (MSC) [23] Cells were taken from healthy humandonors after the approval from the Ethics Committee for Human Research from the University ofGranada (424CEIH2018) Regular Dulbeccorsquos modified Eaglersquos medium (DMEM) with 1 gL glucose(DMEM-LG) (Gibco) 10 fetal bovine serum (FBS) (Sigma-Aldrich St Louis MO USA) 1100 ofnon-essential amino acid solution (NEAA) (Gibco) 001 microgmL of basic fibroblast growth factor (bFGF)
Pharmaceutics 2019 11 388 5 of 18
(PeproTech London UK) 100 UmL of penicillinstreptomycin and 025 microgmL of amphotericin Bwas used as culture medium for all experiments Cultures were maintained at 37 C in a 5 CO2atmosphere (2000 cellswell) All biological experiments were repeated in triplicate at least 3 timesper condition
241 Cell Migration
A cell-migration assay was conducted as previously described [2627] Briefly MSCs weredistributed on to three wells for each condition and allowed to grow to a cell confluency close to99 in 24-wellsplate at 3000 cellscm2 and in each well three dicrarrerent scratches were made Thencells were starved for 24 h by adding culture medium without serum A scratch was made usinga pipette tip along the diameter of the well A wash step with PBS was performed to remove thescratched cells Fresh complete culture media was added and supplemented depending on the assignedgroup (BMP-2 NP- BMP2 and NP-BSA-BMP2 at 125 25 and 5 ngmL of BMP-2) Afterwardsnine images were taken from the same area in each condition until 48 h later On these images thescraped area was measured by ImageJ software (National Institute of Health Bethesda MD USAhttprsbwebnihgovij) The reduction in the scratched area over time was measured consideringthe area at time 0 as 100 open
242 Cell Proliferation
Proliferation was evaluated by a sulphorhodamine (SRB) assay [28] The assay was conducted byseeding the cells at 1500 cellscm2 in a 96-well plate at a confluence not higher than 50 After cellattachment the dicrarrerent supplements were added (BMP-2 NP- BMP2 and NP-BSA-BMP2 at 12525 and 5 ngmL of BMP-2) and the cells were maintained in culture for up to 7 days At each timepoint the cells were washed with 1X PBS and fixed by adding ice-cold 10 trichloroacetic acid for20 min at 4 C Then the cells were washed 3 times with dH2O and dried until all time points werecollected Each well received 04 SRB in 1 acetic acid for 20 min at room temperature with gentleshaking The staining was finished by washing each well 3 times with 1 acetic acid and drying it atroom temperature for 24 h The dye was retrieved from the cells by adding 10 mM Tris Base at pH 105and gently shaking for 10 min The solution recovered was then distributed in a 96-well plate and theoptical absorbance was read at 492 nm
243 Osteogenic Dicrarrerentiation
Osteogenic dicrarrerentiation was evaluated by adding osteogenic media to the cell culture incombination with free BMP-2 NP-BMP2 and NP-BSA-BMP2 at the highest dosages used inprevious experiments Cells were seeded at 3000 cellscm2 and cultured to reach an 85 to90 confluency This was followed by the addition of induction media containing 10 mM of-glycerophosphate (Fluka 50020) 01 microM of dexamethasone (Sigma-Aldrich D2915) and 005 mMof L-ascorbic acid (Sigma-Aldrich A8960) Cell cultures were maintained for 7 days to analyzeearly activity At day 7 cells were collected in 1 mL of TRIzolreg Then RNA was extracted andconverted to cDNA Alkaline phosphatase (ALP) was then evaluated expression being calculatedrelative to glyceraldehyde-3-phosphate dehydrogenase protein (GAPDH) by the 2DDCt methodThese procedures were conducted as described elsewhere [23] Forward and reverse primer sequenceswere AGCTCATTTCCTGGTATGACAAC and TTACTCCTTGGAGGCCATGTG for GAPDH andTCCAGGGATAAAGCAGGTCTTG and CTTTCTCTTTCTCTGGCACTAAGG for ALP
244 Statistical Evaluation
Cell migration and proliferation were evaluated by ANOVA followed by Tukey multiplecomparisons test for pairwise analysis Comparison between the levels of ALP at 4 vs 7 dayswere analyzed by paired Studentrsquos t test In all cases a p value lower than 005 was established asstatistical significance
Pharmaceutics 2019 11 388 6 of 18
3 Results and Discussion
31 Nanoparticle Formulation
Double emulsion-solvent evaporation has been described as a robust and frequently used methodto produce biomolecule-loaded PLGA NPs [6121329] A formulation previously optimized byour group enabled the preservation of the biological activity of encapsulated biomolecules using aslightly aggressive organic solvent Moreover deoxycholic acid has been used in the first step of theformulation in order to improve the colloidal stability of NPs and simultaneously to obtain NP surfacesenriched with carboxylic groups improving their versatility and allowing a subsequent chemicalimmobilization of dicrarrerent specific ligands [30] By means of this improved formulation in the presentwork we developed empty nanoparticles (NPs) or nanoparticles encapsulating rhBMP-2 (NP-BMP2)A schematic description of the synthesis procedure is shown in Figure S1 of the Supplementary DataFor NP-BMP2 we achieved a protein-encapsulation eciency (EE) of 97 plusmn 2 This result is consistentwith the literature in which several authors have reported similarly high values encapsulating thisprotein inside PLGA nano- and microparticles [3132] Our formulation has several factors leading tothis very high EE value The low proteinpolymer relation in mass [33] the anity of rhBMP-2 to anunspecific interaction with hydrophobic surfaces [31] or the addition of stabilizers (poloxamer) inthe second step of the double-emulsion procedure [13] The absence of rhBMP-2 in the supernatantresulting from the centrifugation step in the cleaning process was verified by ELISA and SDS-PAGE inwhich a clear band corresponding to 14 kD of rhBMP-2 polypeptidic chains is shown for lane A inFigure 1 corresponding to NP-BMP2 The mass of protein encapsulated around 2 microg is similar to thatof dicrarrerent PLGA micro- and nanosystems described in the literature [183435] Taking into accountthe storage conditions for our samples this corresponds to 500 ngmL which represents a sucientconcentration for practical applications since this growth factor shows in vitro biological activities atvery low dosages (5ndash20 ngmL) [13]
Pharmaceutics 2019 11 x 6 of 18
31 Nanoparticle Formulation
Double emulsion-solvent evaporation has been described as a robust and frequently used method to produce biomolecule-loaded PLGA NPs [6121329] A formulation previously optimized by our group enabled the preservation of the biological activity of encapsulated biomolecules using a slightly aggressive organic solvent Moreover deoxycholic acid has been used in the first step of the formulation in order to improve the colloidal stability of NPs and simultaneously to obtain NP surfaces enriched with carboxylic groups improving their versatility and allowing a subsequent chemical immobilization of different specific ligands [30] By means of this improved formulation in the present work we developed empty nanoparticles (NPs) or nanoparticles encapsulating rhBMP-2 (NP-BMP2) A schematic description of the synthesis procedure is shown in Figure S1 of the Supplementary Data For NP-BMP2 we achieved a protein-encapsulation efficiency (EE) of 97 plusmn 2 This result is consistent with the literature in which several authors have reported similarly high values encapsulating this protein inside PLGA nano- and microparticles [3132] Our formulation has several factors leading to this very high EE value The low proteinpolymer relation in mass [33] the affinity of rhBMP-2 to an unspecific interaction with hydrophobic surfaces [31] or the addition of stabilizers (poloxamer) in the second step of the double-emulsion procedure [13] The absence of rhBMP-2 in the supernatant resulting from the centrifugation step in the cleaning process was verified by ELISA and SDS-PAGE in which a clear band corresponding to 14 kD of rhBMP-2 polypeptidic chains is shown for lane A in Figure 1 corresponding to NP-BMP2 The mass of protein encapsulated around 2 microg is similar to that of different PLGA micro- and nanosystems described in the literature [183435] Taking into account the storage conditions for our samples this corresponds to 500 ngmL which represents a sufficient concentration for practical applications since this growth factor shows in vitro biological activities at very low dosages (5ndash20 ngmL) [13]
Figure 1 SDS polyacrylamide gel electrophoresis (SDS-PAGE) analysis under reducing conditions of solid PLGA Nanoparticles (PLGA NPs) and liquid (supernatant) fractions of different NP systems Lane P Protein standards lane A NP-BMP2 (bone morphogenetic protein) lane B supernatant of NP-BMP2 after synthesis and encapsulation of rhBMP-2 lane C NP after physical adsorption of BSArhBMP-2 lane D supernatant after physical adsorption of BSA(bovine serum albumin)rhBMP-2 on NP system
On the other hand a second nanosystem resulted modifying the way in which rhBMP-2 is incorporated in the nanocarrier There are several examples of surface adsorption of different growth factors in micro- and nanoparticles [35ndash37] and surface immobilization over the encapsulation has recently been proposed as a way to modulate the later release of biomolecules This process which
Figure 1 SDS polyacrylamide gel electrophoresis (SDS-PAGE) analysis under reducing conditions ofsolid PLGA Nanoparticles (PLGA NPs) and liquid (supernatant) fractions of dicrarrerent NP systems LaneP Protein standards lane A NP-BMP2 (bone morphogenetic protein) lane B supernatant of NP-BMP2after synthesis and encapsulation of rhBMP-2 lane C NP after physical adsorption of BSArhBMP-2lane D supernatant after physical adsorption of BSA(bovine serum albumin)rhBMP-2 on NP system
On the other hand a second nanosystem resulted modifying the way in which rhBMP-2 isincorporated in the nanocarrier There are several examples of surface adsorption of dicrarrerent growthfactors in micro- and nanoparticles [35ndash37] and surface immobilization over the encapsulation has
Pharmaceutics 2019 11 388 7 of 18
recently been proposed as a way to modulate the later release of biomolecules This process whichdepends on the slow dicrarrusion of biomolecules through the polymeric matrix is consequently highlyinfluenced by the proteinndashpolymer interaction [3839] and polymer degradation [36] Thus this newfocus on the use of PLGA NPs for biomolecule delivery was explored by immobilizing the proteinrhBMP-2 on the surface of empty NPs by means of simple physical adsorption This process is knownto be governed by electrostatic and hydrophobic interactions between protein molecules and NPsurfaces [40]
For this the surface-charged groups the hydrophilicity the net charge of the protein moleculesand the characteristics of the adsorption medium are the reference parameters Thus we designed aco-adsorption experiment in which a mixture of rhBMP-2 and BSA (04 ww rhBMP-2BSA) interactsimultaneously with the PLGA NP surface Albumins are routinely used as protective proteins whengrowth factors are incorporated in PLGA NPs [1319] Moreover a surface distribution of BSA moleculescan improve the colloidal stability of NPs at physiological pH due to their net negative charge underthese conditions [41] Figure S2 from Supplementary Materials shows a scheme of the co-adsorptionprocess The adsorption eciency is higher than 95 and in SDS-PAGE from Figure 1 two bandscharacteristic of both proteins can be seen in lane C corresponding to the NP-BSA-BMP2 nanosystemHowever lane D corresponding to the run of the supernatant from the centrifugation of the nanosystemafter adsorption processes shows the absence of any protein This result is fully explained by takinginto account the pH of the medium (pH 50) near the isoelectric point of BSA where the adsorption ofthis protein onto negatively charged nanoparticles presents a maximum [4042] The immobilizationof rhBMP-2 on the negatively charged surface of NPs proves they are electrostatically favored due tothe positive net charge of this protein at acid and neutral pH
32 Nanoparticle Characterization
321 Nanoparticle Size
SEM and STEM micrographs (Figure 2) show that the samples consist of spherical particles ofdicrarrerent diameters (between 150 and 450 nm) a range similar to that found in a previous work inwhich NPs were loaded with lysozyme following a similar synthesis protocol [11] In that workthe DLS technique failed to provide a reliable size distribution Therefore the NTA technique wasdirectly used to determine the hydrodynamic size of the BMP2-loaded NPs (see NTA video in theSupplementary Material)
The size distributions for empty (NP) and BMP-loaded NPs (NP-BMP2) from NTA (Figure 3 andvideos S1 S2) were consistent with the SEM images Particles with diameters between 100 and 500 nmwere found to have the highest particle concentration at around 200 nm The loading with BMP hadan ecrarrect on the size distribution leading to more defined peaks These measurements enabled usto determine the concentration of particles in the measured sample 688 plusmn 009 108 ppmL and519 plusmn 012 108 ppmL for NP and NP-BMP2 nanosystems respectively These values were used(by taking into account the corresponding dilution) to control the number of particles added in thecell experiments
Pharmaceutics 2019 11 388 8 of 18
Pharmaceutics 2019 11 x 7 of 18
depends on the slow diffusion of biomolecules through the polymeric matrix is consequently highly influenced by the proteinndashpolymer interaction [3839] and polymer degradation [36] Thus this new focus on the use of PLGA NPs for biomolecule delivery was explored by immobilizing the protein rhBMP-2 on the surface of empty NPs by means of simple physical adsorption This process is known to be governed by electrostatic and hydrophobic interactions between protein molecules and NP surfaces [40]
For this the surface-charged groups the hydrophilicity the net charge of the protein molecules and the characteristics of the adsorption medium are the reference parameters Thus we designed a co-adsorption experiment in which a mixture of rhBMP-2 and BSA (04 ww rhBMP-2BSA) interact simultaneously with the PLGA NP surface Albumins are routinely used as protective proteins when growth factors are incorporated in PLGA NPs [1319] Moreover a surface distribution of BSA molecules can improve the colloidal stability of NPs at physiological pH due to their net negative charge under these conditions [41] Figure S2 from Supplementary Materials shows a scheme of the co-adsorption process The adsorption efficiency is higher than 95 and in SDS-PAGE from Figure 1 two bands characteristic of both proteins can be seen in lane C corresponding to the NP-BSA-BMP2 nanosystem However lane D corresponding to the run of the supernatant from the centrifugation of the nanosystem after adsorption processes shows the absence of any protein This result is fully explained by taking into account the pH of the medium (pH 50) near the isoelectric point of BSA where the adsorption of this protein onto negatively charged nanoparticles presents a maximum [4042] The immobilization of rhBMP-2 on the negatively charged surface of NPs proves they are electrostatically favored due to the positive net charge of this protein at acid and neutral pH
32 Nanoparticle Characterization
321 Nanoparticle Size
SEM and STEM micrographs (Figure 2) show that the samples consist of spherical particles of different diameters (between 150 and 450 nm) a range similar to that found in a previous work in which NPs were loaded with lysozyme following a similar synthesis protocol [11] In that work the DLS technique failed to provide a reliable size distribution Therefore the NTA technique was directly used to determine the hydrodynamic size of the BMP2-loaded NPs (see NTA video in the Supplementary Material)
Figure 2 Scanning electron microscopy (SEM) micrograph of rhBMP-2-loaded nanoparticles (NP-BMP2)
Figure 2 Scanning electron microscopy (SEM) micrograph of rhBMP-2-loaded nanoparticles(NP-BMP2)
Pharmaceutics 2019 11 x 8 of 18
The size distributions for empty (NP) and BMP-loaded NPs (NP-BMP2) from NTA (Figure 3 and videos S1 S2) were consistent with the SEM images Particles with diameters between 100 and 500 nm were found to have the highest particle concentration at around 200 nm The loading with BMP had an effect on the size distribution leading to more defined peaks These measurements enabled us to determine the concentration of particles in the measured sample 688 plusmn 009 times 108 ppmL and 519 plusmn 012 times 108 ppmL for NP and NP-BMP2 nanosystems respectively These values were used (by taking into account the corresponding dilution) to control the number of particles added in the cell experiments
Figure 3 Hydrodynamic diameter distribution of NP (circles) and NP-BMP2 (thick black line) measured at pH 70 (phosphate buffer) by nanoparticle tracking analysis (NTA)
322 Electrokinetic Mobility and Colloidal Stability
The surface charge of nanoparticles can be analyzed using an electrokinetic study by measuring the electrophoretic mobility (microe) under different conditions Figure 4 shows the microe and zeta potential values for the three nanosystems NP NP-BMP2 and NP-BSA-BMP2 at low ionic strength and different pH values The electric surface charge of NPs resides in the carboxylic groups of the uncapped PLGA and deoxycholic acid molecules These functionalized groups are additionally useful due to the possibility of a chemical surface vectorization in order to develop directed delivery nanocarriers [43] It was previously confirmed that protonation of these acidic surface groups at pH values under their pKa value was tightly correlated with a loss of surface charge and consequently a reduction (in absolute value) of the electrophoretic mobility of the colloidal system [4445] Usually when colloidal particles are coated by protein molecules the microe values change markedly compared with the same bare surfaces and are influenced by the electrical charge of the adsorbed protein molecules [4647] The electrokinetic behavior of the NP-BMP2 system remains similar to that of NP and encapsulation of rhBMP-2 does not affect the surface charge distribution A similar result was reported by drsquoAngelo et al on encapsulating different growth factors in PLGA-poloxamer blend nanoparticles in the same proportion ww of proteinpolymer [24] This may be due to the low amount of encapsulated protein and its distribution in the inner part of the NPs (far from the surface) In our system this internal distribution may be favored by the encapsulating conditions where the basic pH (pH 120) of the water phase containing rhBMP-2 allows a negative charge of these protein molecules thereby preventing their electrostatic specific interaction with acidic groups of the NPs
0 200 400 600 80000
05
10
15
20
25
30
35
40
parti
cle
conc
entra
tion
(106 p
pm
L)
D(nm)
Figure 3 Hydrodynamic diameter distribution of NP (circles) and NP-BMP2 (thick black line) measuredat pH 70 (phosphate bucrarrer) by nanoparticle tracking analysis (NTA)
322 Electrokinetic Mobility and Colloidal Stability
The surface charge of nanoparticles can be analyzed using an electrokinetic study by measuringthe electrophoretic mobility (microe) under dicrarrerent conditions Figure 4 shows the microe and zeta potentialvalues for the three nanosystems NP NP-BMP2 and NP-BSA-BMP2 at low ionic strength anddicrarrerent pH values The electric surface charge of NPs resides in the carboxylic groups of the uncappedPLGA and deoxycholic acid molecules These functionalized groups are additionally useful due to thepossibility of a chemical surface vectorization in order to develop directed delivery nanocarriers [43]It was previously confirmed that protonation of these acidic surface groups at pH values undertheir pKa value was tightly correlated with a loss of surface charge and consequently a reduction (in
Pharmaceutics 2019 11 388 9 of 18
absolute value) of the electrophoretic mobility of the colloidal system [4445] Usually when colloidalparticles are coated by protein molecules the microe values change markedly compared with the samebare surfaces and are influenced by the electrical charge of the adsorbed protein molecules [4647]The electrokinetic behavior of the NP-BMP2 system remains similar to that of NP and encapsulationof rhBMP-2 does not acrarrect the surface charge distribution A similar result was reported by drsquoAngeloet al on encapsulating dicrarrerent growth factors in PLGA-poloxamer blend nanoparticles in the sameproportion ww of proteinpolymer [24] This may be due to the low amount of encapsulated proteinand its distribution in the inner part of the NPs (far from the surface) In our system this internaldistribution may be favored by the encapsulating conditions where the basic pH (pH 120) of the waterphase containing rhBMP-2 allows a negative charge of these protein molecules thereby preventingtheir electrostatic specific interaction with acidic groups of the NPs
Pharmaceutics 2019 11 x 9 of 18
Figure 4 Electrophoretic mobility and zeta potential vs pH in buffered media of low salinity (ionic strength equal to 0002 M) for the different nanosystems (black square) NP (blue triangle) NP-BMP2 (red circle) NP-BSA-BMP2
The electrokinetic distribution for the NP-BSA-BMP2 system radically changes As previously shown the very high adsorption efficiency leads to NPs with both proteins adsorbed around their surface This situation is closely correlated with the microe values from Figure 4 Taking into account the ww relation between adsorbed proteins (250 times higher for BSA) albumin molecules modulate the behavior at pH values below their isoelectric point (pI 47) where the positive net charge of BSA masks the original surface charge of NPs and even changes their original values to positive ones This is a typical result found for this protein-covering colloidal particles [4248] At neutral and basic pH values BSA molecules have a negative net charge and the slight decrease in the absolute microe values could be due to the reduction of the negative net surface charge of NPs which may be shielded at least in a small part by the positive charge of rhBMP-2 molecules under their basic isoelectric point (pI 90)
The colloidal stability for the different nanosystems (NP NP-BMP2 and NP-BSA-BMP2) was determined by analyzing the size distributions in various media (PB PBS and DMEM) at different times after synthesis (0 1 and 5 days) Size distributions similar to the original ones were found for the two formulations NP and NP-BMP2 in all the media analyzed This result was similar to that previously found for these types of NPs encapsulating lysozyme [11] in which the combination of electrostatic and steric interactions generated by surface chemical groups of NPs confer the stability mechanism that prevents colloidal aggregation [33] The decrease of the absolute value of the zeta potential for the NP-BSA-BMP2 system as a consequence of surface protein distribution does not affect its colloidal stability This system also maintains the same size distribution in the different media It is commonly accepted that a zeta potential higher than +30 or minus30 mV will give rise to a stable colloidal system [49] and the zeta potential value for NP-BSA-BMP2 is above minus30 mV Colloidal stability in PBS and DMEM typically used media for the development of scaffold or cell interactions
Figure 4 Electrophoretic mobility and zeta potential vs pH in bucrarrered media of low salinity (ionicstrength equal to 0002 M) for the dicrarrerent nanosystems (black square) NP (blue triangle) NP-BMP2(red circle) NP-BSA-BMP2
The electrokinetic distribution for the NP-BSA-BMP2 system radically changes As previouslyshown the very high adsorption eciency leads to NPs with both proteins adsorbed around theirsurface This situation is closely correlated with the microe values from Figure 4 Taking into account theww relation between adsorbed proteins (250 times higher for BSA) albumin molecules modulate thebehavior at pH values below their isoelectric point (pI 47) where the positive net charge of BSA masksthe original surface charge of NPs and even changes their original values to positive ones This is atypical result found for this protein-covering colloidal particles [4248] At neutral and basic pH valuesBSA molecules have a negative net charge and the slight decrease in the absolute microe values could bedue to the reduction of the negative net surface charge of NPs which may be shielded at least in asmall part by the positive charge of rhBMP-2 molecules under their basic isoelectric point (pI 90)
The colloidal stability for the dicrarrerent nanosystems (NP NP-BMP2 and NP-BSA-BMP2) wasdetermined by analyzing the size distributions in various media (PB PBS and DMEM) at dicrarrerenttimes after synthesis (0 1 and 5 days) Size distributions similar to the original ones were found for
Pharmaceutics 2019 11 388 10 of 18
the two formulations NP and NP-BMP2 in all the media analyzed This result was similar to thatpreviously found for these types of NPs encapsulating lysozyme [11] in which the combination ofelectrostatic and steric interactions generated by surface chemical groups of NPs confer the stabilitymechanism that prevents colloidal aggregation [33] The decrease of the absolute value of the zetapotential for the NP-BSA-BMP2 system as a consequence of surface protein distribution does not acrarrectits colloidal stability This system also maintains the same size distribution in the dicrarrerent media It iscommonly accepted that a zeta potential higher than +30 or 30 mV will give rise to a stable colloidalsystem [49] and the zeta potential value for NP-BSA-BMP2 is above 30 mV Colloidal stability in PBSand DMEM typically used media for the development of scacrarrold or cell interactions respectivelyassures the potential use of these nanosystems for in vitro or in vivo living environments Additionallythese systems maintained their size under storage in PB at 4 C for at least 1 month (data not shown)showing this to be an adequate medium for sample storage
323 Protein Release
One of the main problems for micro- or nanosystems of PLGA drug delivery is to find theappropriate release pattern for encapsulatedattached protein molecules A wide spectrum offormulations modulates this property by the use of dicrarrerent types of synthesis processes PLGApolymers co-polymers and stabilizers [313] An adequate limitation and control in the burst releaseis critical for BMPs in order to ensure long-term continuous release that favored by the polymerdegradation provides better in vivo action in driving bone and cartilage regeneration [20] Thereforewe previously developed a dual PLGA nanosystem for controlled short-term release where proteindicrarrusion and proteinndashpolymer interaction are the main factors governing this process [11]
In the present work NP-BMP2 and NP-BSA-BMP2 nanosystems represent two dicrarrerent ways inwhich rhBMP-2 was incorporated into the nanocarrier Figure 5A shows the cumulative release of bothproteins rhBMP-2 and BSA for dicrarrerent systems as a function of time in a short-term period (7 days)The encapsulated rhBMP-2 protein reaches an amount released of around 30 of the initial encapsulatedone while adsorbed rhBMP-2 despite its surface distribution is three times lower However BSAshows released amounts up to 80 of the initial adsorbed ones In all cases error bars correspond tothe standard deviations from three independent experiments Under these conditions the growthfactor encapsulated in NP-BMP2 presents a release pattern similar to that previously found with thesame formulation but using lysozyme as the protein [11] Poloxamer in the water phase of the synthesisprocess can be key in modulating both specific and unspecific interfacial protein interactions [50]Thus the relation between proteinndashpolymer interaction and protein dicrarrusion appears to be wellbalanced preventing an excessive initial burst and simultaneously maintaining the needed proteinflux to release around a third of the encapsulated rhBMP-2 in 7 days Although an excessive initialburst has been widely reported for PLGA NPs related with protein molecules close to the surface [6]this situation did not appear for the NP-BMP2 system this being consistent with the electrokineticbehavior that did not show the presence of protein near surface The literature ocrarrers some exampleswith reduced short-term release of BMP-2 using more hydrophilic PLGA-PEG co-polymers [16] or adicrarrerent synthesis process [25]
The release performance for the NP-BSA-BMP2 system also shown in Figure 5A presents notabledicrarrerences The electrokinetic profile has previously justified the surface location of BSA and rhBMP-2on the surface which could lead to a fast release of both proteins However results from Figure 5ABshow this trend only for the BSA protein that is released from NPs with about 20 of the initial amountremaining after seven days However up to 90 of the initial load of rhBMP-2 protein unlike BSAremains attached to the surface The NP surface with hydrophilic groups form poloxamer moleculesand a negative charge due to the abundant presence of carboxylic groups (end-groups of PLGA anddeoxycholic acid molecules) favor a desorption process for BSA whose molecules have a negativecharge under release conditions (physiological pH) This agrees with the results of other authors whoeven after encapsulating BSA in PLGA-poloxamer blend NPs achieved a fast burst release of above
Pharmaceutics 2019 11 388 11 of 18
40 to 50 of the initial protein amount [33] Moreover the co-encapsulation of albumins with growthfactors could strongly acrarrect its release profile causing an initial burst [2124] Otherwise the specificelectrostatic attraction between positive rhBMP-2 molecules and negative surface groups slows downthe short time release of this protein This result is in agreement with the low release of adsorbedBMP previously found using PLGA micro- and nanoparticles with uncapped acid end groups [3851]Thus the combination of dicrarrerent methods for trapping BMP-2 into and around NPs shows up thepossibility of attaining a properly controlled release balancing the interactions between polymersstabilizers and protein
Pharmaceutics 2019 11 x 10 of 18
respectively assures the potential use of these nanosystems for in vitro or in vivo living
environments Additionally these systems maintained their size under storage in PB at 4 degC for at
least 1 month (data not shown) showing this to be an adequate medium for sample storage
323 Protein Release
One of the main problems for micro- or nanosystems of PLGA drug delivery is to find the
appropriate release pattern for encapsulatedattached protein molecules A wide spectrum of
formulations modulates this property by the use of different types of synthesis processes PLGA
polymers co-polymers and stabilizers [313] An adequate limitation and control in the burst release
is critical for BMPs in order to ensure long-term continuous release that favored by the polymer
degradation provides better in vivo action in driving bone and cartilage regeneration [20] Therefore
we previously developed a dual PLGA nanosystem for controlled short-term release where protein
diffusion and proteinndashpolymer interaction are the main factors governing this process [11]
In the present work NP-BMP2 and NP-BSA-BMP2 nanosystems represent two different ways
in which rhBMP-2 was incorporated into the nanocarrier Figure 5A shows the cumulative release of
both proteins rhBMP-2 and BSA for different systems as a function of time in a short-term period (7
days) The encapsulated rhBMP-2 protein reaches an amount released of around 30 of the initial
encapsulated one while adsorbed rhBMP-2 despite its surface distribution is three times lower
However BSA shows released amounts up to 80 of the initial adsorbed ones In all cases error bars
correspond to the standard deviations from three independent experiments Under these conditions
the growth factor encapsulated in NP-BMP2 presents a release pattern similar to that previously
found with the same formulation but using lysozyme as the protein [11] Poloxamer in the water
phase of the synthesis process can be key in modulating both specific and unspecific interfacial
protein interactions [50] Thus the relation between proteinndashpolymer interaction and protein
diffusion appears to be well balanced preventing an excessive initial burst and simultaneously
maintaining the needed protein flux to release around a third of the encapsulated rhBMP-2 in 7 days
Although an excessive initial burst has been widely reported for PLGA NPs related with protein
molecules close to the surface [6] this situation did not appear for the NP-BMP2 system this being
consistent with the electrokinetic behavior that did not show the presence of protein near surface
The literature offers some examples with reduced short-term release of BMP-2 using more
hydrophilic PLGA-PEG co-polymers [16] or a different synthesis process [25]
(A) (B)
Figure 5 (A) Cumulative release of rhBMP-2 for NP-BMP2 (black square) and NP-BSA-BMP2 (red
circle) systems and cumulative release of BSA for NP-BSA-BMP2 (blue triangle) system incubated
for different times at 37 degC in saline phosphate buffer (pH 74) (B) SDS-PAGE analysis under reducing
0 20 40 60 80 100 120 140 160 180
0
20
40
60
80
100
Cum
ulat
ive
rele
ase
()
Time (hours)
Figure 5 (A) Cumulative release of rhBMP-2 for NP-BMP2 (black square) and NP-BSA-BMP2 (redcircle) systems and cumulative release of BSA for NP-BSA-BMP2 (blue triangle) system incubated fordicrarrerent times at 37 C in saline phosphate bucrarrer (pH 74) (B) SDS-PAGE analysis under reducingconditions of solid fraction of NP-BSA-BMP2 after release at dicrarrerent times where the number of eachlane corresponds to the time in hours
33 Biological Activity and Interactions
331 Cell Migration
Cell migration is the first and necessary step in tissue regeneration [52] Thus a regenerativeagent must accelerate cell migration or at least not interfere with it In the present study we found nodicrarrerences between the groups doses and control in terms of closure of a scratched area (ANOVAwith Tukey multiple comparisons test) (Figure 6) In contrast to our findings previously publisheddata suggests a positive ecrarrect of BMP-2 on cell migration [5354] However in those studies the dosesapplied and the cell types were dicrarrerent than in the current experiments We used lower doses ofBMP-2 in order to test whether even at low dosages BMP-2 could still provide benefits if protectedin a nanoparticle system As mentioned we demonstrated no negative ecrarrect of the system on cellmigration Our results nonetheless support the idea that BMP-2 activity is mediated by the activation ofthe phosphoinositide 3-kinase (PI3K) pathway a common group of signaling molecules that participatein several process with BMP-2 and other molecules [2654] It should also be mentioned that thetimeframe of a migration assay is short Thus the potential advantages of a controlled-release systemas the one under study might be limited That is the release of BMP-2 from the nanoparticles asdemonstrated in Figure 5 is limited to the first 48 h Thus a sustained positive ecrarrect on migrationactivity over time could be hypothesized
Pharmaceutics 2019 11 388 12 of 18Pharmaceutics 2019 11 x 12 of 18
Figure 6 Migration assay Percentage of scratched area closure at 24 and 48 h on different groups and doses
332 Cell Proliferation
Proliferation is another of the cell activities required for tissue regeneration However this property must be balanced with both migration and differentiation and not all three characteristics increase at the same time and with the same ratios [55] In fact reportedly when a dose of BMP-2 induces higher proliferation it decreases differentiation [56] This property has been extensively analyzed but discrepancies can still be detected in the literature Therefore Kim et al analyzed different doses of BMP-2 and its effect on cell proliferation and apoptosis It was confirmed in vitro that high doses but still lower than those used clinically reduce cell proliferation and increase apoptosis [57] This should be avoided We have found that although free BMP-2 does not induce higher proliferation than the control at any of the doses applied nor time points (ANOVA with Tukey multiple comparisons test) the same amount of BMP-2 encapsulated or adsorbed onto PLGA nanoparticles boosts proliferation this being statistically significant when using a dose of 25 ngmL or higher (ANOVA with Tukey multiple comparisons test) (Figure 7) These dosages are still lower than those suggested in previous studies Apart from that difference a positive effect on proliferation was still achieved Moreover following the release pattern from Figure 5 more BMP-2 is expected to be released over time beyond the 7-day time frame Thus a sustained induction effect could be expected as well until full confluency of the cell culture
0
10
20
30
40
50
60
70
80
90
100
0 h 24 h 48 h
o
f scr
atch
ed a
rea
Time point
Control
BMP2-125 ngmL
BMP2-25 ngmL
BMP2-5 ngmL
NP-BMP2-125 ngmL
NP-BMP2-25 ngmL
NP-BMP2-5 ngmL
NP-BSA-BMP2-125 ngmL
NP-BSA-BMP2-25 ngmL
NP-BSA-BMP2-5 ngmL
Figure 6 Migration assay Percentage of scratched area closure at 24 and 48 h on dicrarrerent groupsand doses
332 Cell Proliferation
Proliferation is another of the cell activities required for tissue regeneration However this propertymust be balanced with both migration and dicrarrerentiation and not all three characteristics increaseat the same time and with the same ratios [55] In fact reportedly when a dose of BMP-2 induceshigher proliferation it decreases dicrarrerentiation [56] This property has been extensively analyzed butdiscrepancies can still be detected in the literature Therefore Kim et al analyzed dicrarrerent doses ofBMP-2 and its ecrarrect on cell proliferation and apoptosis It was confirmed in vitro that high doses butstill lower than those used clinically reduce cell proliferation and increase apoptosis [57] This shouldbe avoided We have found that although free BMP-2 does not induce higher proliferation than thecontrol at any of the doses applied nor time points (ANOVA with Tukey multiple comparisons test)the same amount of BMP-2 encapsulated or adsorbed onto PLGA nanoparticles boosts proliferationthis being statistically significant when using a dose of 25 ngmL or higher (ANOVA with Tukeymultiple comparisons test) (Figure 7) These dosages are still lower than those suggested in previousstudies Apart from that dicrarrerence a positive ecrarrect on proliferation was still achieved Moreoverfollowing the release pattern from Figure 5 more BMP-2 is expected to be released over time beyondthe 7-day time frame Thus a sustained induction ecrarrect could be expected as well until full confluencyof the cell culture
Pharmaceutics 2019 11 388 13 of 18Pharmaceutics 2019 11 x 13 of 18
Figure 7 Proliferation of human mesenchymal stromal cells (MSCs) as measured by sulphorhodamine (SRB) absorbance Results were normalized to T0 in each group
333 Osteogenic Differentiation
It has been confirmed that cell differentiation induced by BMP-2 needs the presence of permissive osteoinductive components Particularly β-glycerophosphate has been shown to exert a synergistic effect with BMP-2 in inducing cell differentiation [56] Thus to test for osteogenic differentiation we analyzed the expression of ALP mRNA Maximum ALP activity was found to occur 10 days after stimulation with PLGA-based microparticles containing BMP-2 in co-encapsulation with human serum albumin [16] Although other tests could have been used to reinforce our findings ALP is known to modulate the deposition of mineralized nodules thus indicating osteoblastic activity For all of this we supplemented the differentiation media with β-glycerophosphate and either free BMP-2 NP-BMP2 or NP-BSA-BMP2 for 4 and 7 days so that we could capture the early dynamics of the expression of the gene In our study we identified an increase in the expression of ALP in all groups from day 4 to day 7 (Figure 8) Although ALP at day 7 in the BMP-2 group appears to be higher than for the other two groups the change did not prove significant In fact differences between groups were not statistically significant within any time period Noteworthy though the increase was not significant within the BMP-2 group (p = 0141 Studentrsquos t test) but it was significant within the other two groups (p = 0025 and p = 0003 NP-BMP2 and NP-BSA-BMP2 groups respectively) This again could be taken as a confirmation of the sustained release of the protein from the nanoparticle system beyond the earlier time points
This and both the migration and proliferation studies described below lead us to confirm that the system proposed can maintain a proper release of BMP-2 over time sustaining a positive effect on cell migration and proliferation with initial reduced doses of BMP-2 The fact that the excessive initial burst is prevented is important for the application of this nanotechnology in bone regeneration as in dentistry In this way the negative effects of initial high doses of BMP-2 are avoided at the same time as the molecule is protected from denaturalization inside the NP Thus the regenerator effects are maintained over time
T0 T1 T2 T3 T4 T6 T70
2
4
6
Time point
Nor
mal
ized
Abs
orba
nce
NP-BSA-BMP2-125 ngmL
NP-BSA-BMP2-25 ngmL
NP-BSA-BMP2-5 ngmL
NP-BMP2-125 ngmL
NP-BMP2-25 ngmL
NP-BMP2-5 ngmL
BMP2-125 ngmL
BMP2-25 ngmL
BMP2-5 ngmL
Control
Figure 7 Proliferation of human mesenchymal stromal cells (MSCs) as measured by sulphorhodamine(SRB) absorbance Results were normalized to T0 in each group
333 Osteogenic Dicrarrerentiation
It has been confirmed that cell dicrarrerentiation induced by BMP-2 needs the presence of permissiveosteoinductive components Particularly -glycerophosphate has been shown to exert a synergisticecrarrect with BMP-2 in inducing cell dicrarrerentiation [56] Thus to test for osteogenic dicrarrerentiation weanalyzed the expression of ALP mRNA Maximum ALP activity was found to occur 10 days afterstimulation with PLGA-based microparticles containing BMP-2 in co-encapsulation with human serumalbumin [16] Although other tests could have been used to reinforce our findings ALP is known tomodulate the deposition of mineralized nodules thus indicating osteoblastic activity For all of this wesupplemented the dicrarrerentiation media with -glycerophosphate and either free BMP-2 NP-BMP2 orNP-BSA-BMP2 for 4 and 7 days so that we could capture the early dynamics of the expression of thegene In our study we identified an increase in the expression of ALP in all groups from day 4 to day 7(Figure 8) Although ALP at day 7 in the BMP-2 group appears to be higher than for the other twogroups the change did not prove significant In fact dicrarrerences between groups were not statisticallysignificant within any time period Noteworthy though the increase was not significant within theBMP-2 group (p = 0141 Studentrsquos t test) but it was significant within the other two groups (p = 0025and p = 0003 NP-BMP2 and NP-BSA-BMP2 groups respectively) This again could be taken as aconfirmation of the sustained release of the protein from the nanoparticle system beyond the earliertime points
This and both the migration and proliferation studies described below lead us to confirm that thesystem proposed can maintain a proper release of BMP-2 over time sustaining a positive ecrarrect on cellmigration and proliferation with initial reduced doses of BMP-2 The fact that the excessive initialburst is prevented is important for the application of this nanotechnology in bone regeneration asin dentistry In this way the negative ecrarrects of initial high doses of BMP-2 are avoided at the sametime as the molecule is protected from denaturalization inside the NP Thus the regenerator ecrarrects aremaintained over time
Pharmaceutics 2019 11 388 14 of 18
14 of 18
4 Conclusions
In this work a delivery PLGA-nanosystem previously developed for model proteins was chosen as the reference system to carry and deliver the growth factor BMP-2 This NP system with a dual size distribution was developed following a double-emulsion formulation in which the process and the components used were optimized to reach the appropriate colloidal and biological behavior Encapsulation and adsorption are two different processes to load BMP-2 in PLGA NPs Both were tested to elucidate the factors controlling them and their influence in the physico-chemical and biological properties of nanosystems We verified that proteinndashpolymer specific interactions have a major role in the way that protein molecules are carried and delivered from NPs In vitro experiments showed that BMP-2-loaded PLGA NPs are the nanocarriers with the best release profile over the short-term without an initial burst and with moderate and sustained release of active protein before the onset of polymer degradation Therefore the biological activity is positive with no negative interaction with migration or proliferation but rather the induction of cell differentiation through the expression of ALP
Supplementary Materials The following are available online at wwwmdpicomxxxs1 Figure S1 Scheme of the formulation of NP-BMP2 Figure S2 Scheme of the protein adsorption process for NP-BSA-BMP2 Video S1 NTA experiments for NP-BMP2 Video S2 NTA experiments for empty NPs
Author Contributions Conceptualization JMP-G and PG-M methodology JMP-G ABJ-R and MP-M investigation TdC-S IO-O JMP-G ABJ-R and MP-M resources ABJ-R PG-M FO-R writingmdashoriginal draft preparation JMP-G and MP-M writingmdashreview and editing JMP-G MP-M ABJ-R TdC-S supervision JMP-G PG-M and FO-R funding acquisition ABJ-R and PG-M
Funding This research was funded by the Consejeriacutea de Economiacutea Innovacioacuten Ciencia y Empleo de la Junta de Andaluciacutea (Spain) through research groups FQM-115 and CTS-1028 by the following research project MAT2013-43922-RmdashEuropean FEDER support includedmdash(MICINN Spain) and by MIS Ibeacuterica SL
Acknowledgments The authors wish to express their appreciation for the technical support to D Dariacuteo Abril-Garciacutea
Conflicts of Interest The authors declare no conflict of interest
Figure 8 Relative fold change in the expression of ALP mRNA (control group BMP2 at 4 days) =Statistical significance of the comparison over time (p = 0025 and p = 0003 Studentrsquos t test NP-BMP2and NP-BSA-BMP2 groups)
4 Conclusions
In this work a delivery PLGA-nanosystem previously developed for model proteins was chosenas the reference system to carry and deliver the growth factor BMP-2 This NP system with a dualsize distribution was developed following a double-emulsion formulation in which the processand the components used were optimized to reach the appropriate colloidal and biological behaviorEncapsulation and adsorption are two dicrarrerent processes to load BMP-2 in PLGA NPs Both were testedto elucidate the factors controlling them and their influence in the physico-chemical and biologicalproperties of nanosystems We verified that proteinndashpolymer specific interactions have a major role inthe way that protein molecules are carried and delivered from NPs In vitro experiments showed thatBMP-2-loaded PLGA NPs are the nanocarriers with the best release profile over the short-term withoutan initial burst and with moderate and sustained release of active protein before the onset of polymerdegradation Therefore the biological activity is positive with no negative interaction with migrationor proliferation but rather the induction of cell dicrarrerentiation through the expression of ALP
Supplementary Materials The following are available online at httpwwwmdpicom1999-4923118388s1Figure S1 Scheme of the formulation of NP-BMP2 Figure S2 Scheme of the protein adsorption process forNP-BSA-BMP2 Video S1 NTA experiments for NP-BMP2 Video S2 NTA experiments for empty NPs
Author Contributions Conceptualization JMP-G and PG-M methodology JMP-G ABJ-R andMP-M investigation TdC-S IO-O JMP-G ABJ-R and MP-M resources ABJ-R PG-M FO-Rwritingmdashoriginal draft preparation JMP-G and MP-M writingmdashreview and editing JMP-G MP-MABJ-R TdC-S supervision JMP-G PG-M and FO-R funding acquisition ABJ-R and PG-M
Funding This research was funded by the Consejeriacutea de Economiacutea Innovacioacuten Ciencia y Empleo de la Juntade Andaluciacutea (Spain) through research groups FQM-115 and CTS-1028 by the following research projectMAT2013-43922-RmdashEuropean FEDER support includedmdash(MICINN Spain) and by MIS Ibeacuterica SL
Acknowledgments The authors wish to express their appreciation for the technical support to D DariacuteoAbril-Garciacutea
Conflicts of Interest The authors declare no conflict of interest
Pharmaceutics 2019 11 388 15 of 18
References
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2 Kumar B Jalodia K Kumar P Gautam HK Recent advances in nanoparticle-mediated drug delivery JDrug Deliv Sci Technol 2017 41 260ndash268 [CrossRef]
3 Mir M Ahmed N Rehman AUR Recent applications of PLGA based nanostructures in drug deliveryColloids Surf B Biointerfaces 2017 159 217ndash231 [CrossRef] [PubMed]
4 Jana S Jana S Natural polymeric biodegradable nanoblend for macromolecules delivery In RecentDevelopments in Polymer Macro Micro and Nano Blends Woodhead Publishing Cambridge UK 2017pp 289ndash312 ISBN 9780081004081
5 Danhier F Ansorena E Silva JM Coco R Le Breton A Preacuteat V PLGA-based nanoparticles Anoverview of biomedical applications J Control Release 2012 161 505ndash522 [CrossRef] [PubMed]
6 Ding D Zhu Q Recent advances of PLGA micronanoparticles for the delivery of biomacromoleculartherapeutics Mater Sci Eng C 2018 92 1041ndash1060 [CrossRef] [PubMed]
7 Arias JL Unciti-Broceta JD Maceira J del Castillo T Hernaacutendez-Quero J Magez SSoriano M Garciacutea-Salcedo JA Nanobody conjugated PLGA nanoparticles for active targeting of AfricanTrypanosomiasis J Control Release 2015 197 190ndash198 [CrossRef]
8 Giteau A Venier-Julienne MC Aubert-Poueumlssel A Benoit JP How to achieve sustained and completeprotein release from PLGA-based microparticles Int J Pharm 2008 350 14ndash26 [CrossRef]
9 Fredenberg S Wahlgren M Reslow M Axelsson A The mechanisms of drug release inpoly(lactic-co-glycolic acid)-based drug delivery systemsmdashA review Int J Pharm 2011 415 34ndash52[CrossRef]
10 White LJ Kirby GTS Cox HC Qodratnama R Qutachi O Rose FRAJ Shakeshecrarr KM Acceleratingprotein release from microparticles for regenerative medicine applications Mater Sci Eng C 2013 332578ndash2583 [CrossRef]
11 Ortega-Oller I del Castillo-Santaella T Padial-Molina M Galindo-Moreno P Joacutedar-Reyes ABPeula-Garciacutea JM Dual delivery nanosystem for biomolecules Formulation characterization and in vitrorelease Colloids Surf B Biointerfaces 2017 159 586ndash595 [CrossRef]
12 McClements DJ Encapsulation protection and delivery of bioactive proteins and peptides usingnanoparticle and microparticle systems A review Adv Colloid Interface Sci 2018 253 1ndash22 [CrossRef]
13 Ortega-Oller I Padial-Molina M Galindo-Moreno P OrsquoValle F Joacutedar-Reyes AB Peula-Garciacutea JMBone Regeneration from PLGA Micro-Nanoparticles BioMed Res Int 2015 2015 1ndash18 [CrossRef] [PubMed]
14 Bapat RA Joshi CP Bapat P Chaubal TV Pandurangappa R Jnanendrappa N Gorain B Khurana SKesharwani P The use of nanoparticles as biomaterials in dentistry Drug Discov Today 2019 24 85ndash98[CrossRef]
15 Ji Y Xu GP Zhang ZP Xia JJ Yan JL Pan SH BMP-2PLGA delayed-release microspheres compositegraft selection of bone particulate diameters and prevention of aseptic inflammation for bone tissueengineering Ann BioMed Eng 2010 38 632ndash639 [CrossRef] [PubMed]
16 Kirby GTS White LJ Rahman CV Cox HC Qutachi O Rose FRAJ Hutmacher DWShakeshecrarr KM Woodrucrarr MA PLGA-Based Microparticles for the Sustained Release of BMP-2 Polymers2011 3 571ndash586 [CrossRef]
17 Qutachi O Shakeshecrarr KM Buttery LDK Delivery of definable number of drug or growth factor loadedpoly(dl-lactic acid-co-glycolic acid) microparticles within human embryonic stem cell derived aggregates JControl Release 2013 168 18ndash27 [CrossRef] [PubMed]
18 Wang Y Wei Y Zhang X Xu M Liu F Ma Q Cai Q Deng X PLGAPDLLA core-shell submicronspheres sequential release system Preparation characterization and promotion of bone regeneration in vitroand in vivo Chem Eng J 2015 273 490ndash501 [CrossRef]
Pharmaceutics 2019 11 388 16 of 18
19 Zhang H-X Zhang X-P Xiao G-Y Hou -Y Cheng L Si M Wang S-S Li Y-H Nie L In vitroand in vivo evaluation of calcium phosphate composite scacrarrolds containing BMP-VEGF loaded PLGAmicrospheres for the treatment of avascular necrosis of the femoral head Mater Sci Eng C 2016 60 298ndash307[CrossRef]
20 Begam H Nandi SK Kundu B Chanda A Strategies for delivering bone morphogenetic protein forbone healing Mater Sci Eng C 2017 70 856ndash869 [CrossRef]
21 Balmayor ER Feichtinger GA Azevedo HS Van Griensven M Reis RL Starch-poly--caprolactonemicroparticles reduce the needed amount of BMP-2 Clin Orthop Relat Res 2009 467 3138ndash3148 [CrossRef]
22 Xu Y Kim CS Saylor DM Koo D Polymer degradation and drug delivery in PLGA-based drugndashpolymerapplications A review of experiments and theories J BioMed Mater Res Part B Appl Biomater 2017 1051692ndash1716 [CrossRef] [PubMed]
23 Padial-Molina M de Buitrago JG Sainz-Urruela R Abril-Garcia D Anderson P OrsquoValle FGalindo-Moreno P Expression of Musashi-1 during osteogenic dicrarrerentiation of oral MSC An in vitro studyInt J Mol Sci 2019 20 2171 [CrossRef] [PubMed]
24 DrsquoAngelo I Garcia-Fuentes M Parajoacute Y Welle A Vaacutentus T Horvaacuteth A Boumlkoumlnyi G Keacuteri GAlonso MJ Nanoparticles based on PLGApoloxamer blends for the delivery of proangiogenic growthfactors Mol Pharm 2010 7 1724ndash1733 [CrossRef] [PubMed]
25 Chang H-C Yang C Feng F Lin F-H Wang C-H Chang P-C Bone morphogeneticprotein-2 loaded poly(DL-lactide-co-glycolide) microspheres enhance osteogenic potential ofgelatinhydroxyapatite-tricalcium phosphate cryogel composite for alveolar ridge augmentation JFormos Med Assoc 2017 116 973ndash981 [CrossRef] [PubMed]
26 Padial-Molina M Volk SL Rios HF Periostin increases migration and proliferation of humanperiodontal ligament fibroblasts challenged by tumor necrosis factor -crarr and Porphyromonas gingivalislipopolysaccharides J Periodontal Res 2014 49 405ndash414 [CrossRef] [PubMed]
27 Liang C-C Park AY Guan J-L In vitro scratch assay A convenient and inexpensive method for analysisof cell migration in vitro Nat Protoc 2007 2 329ndash333 [CrossRef]
28 Houghton P Fang R Techatanawat I Steventon G Hylands PJ Lee CC The sulphorhodamine (SRB)assay and other approaches to testing plant extracts and derived compounds for activities related to reputedanticancer activity Methods 2007 42 377ndash387 [CrossRef]
29 Iqbal M Zafar N Fessi H Elaissari A Double emulsion solvent evaporation techniques used for drugencapsulation Int J Pharm 2015 496 173ndash190 [CrossRef]
30 Saacutenchez-Moreno P Ortega-Vinuesa JL Boulaiz H Marchal JA Peula-Garciacutea JM Synthesis andcharacterization of lipid immuno-nanocapsules for directed drug delivery Selective antitumor activityagainst HER2 positive breast-cancer cells Biomacromolecules 2013 14 4248ndash4259 [CrossRef]
31 Lochmann A Nitzsche H von Einem S Schwarz E Maumlder K The influence of covalently linked andfree polyethylene glycol on the structural and release properties of rhBMP-2 loaded microspheres J ControlRelease 2010 147 92ndash100 [CrossRef]
32 Kempen DHR Lu L Hecrarreran TE Creemers LB Maran A Classic KL Dhert WJA Yaszemski MJRetention of in vitro and in vivo BMP-2 bioactivities in sustained delivery vehicles for bone tissue engineeringBiomaterials 2008 29 3245ndash3252 [CrossRef]
33 Santander-Ortega MJ Csaba N Gonzaacutelez L Bastos-Gonzaacutelez D Ortega-Vinuesa JL Alonso MJProtein-loaded PLGAndashPEO blend nanoparticles Encapsulation release and degradation characteristicsColloid Polym Sci 2010 288 141ndash150 [CrossRef]
34 Chung YI Ahn KM Jeon SH Lee SY Lee JH Tae G Enhanced bone regeneration with BMP-2loaded functional nanoparticle-hydrogel complex J Control Release 2007 121 91ndash99 [CrossRef]
35 La W-G Kang S-W Yang HS Bhang SH Lee SH Park J-H Kim B-S The Ecacy of BoneMorphogenetic Protein-2 Depends on Its Mode of Delivery Artif Organs 2010 34 1150ndash1153 [CrossRef]
36 Fu Y Du L Wang Q Liao W Jin Y Dong A Chen C Li Z In vitro sustained release of recombinanthuman bone morphogenetic protein-2 microspheres embedded in thermosensitive hydrogels Die Pharm2012 67 299ndash303 [CrossRef]
Pharmaceutics 2019 11 388 17 of 18
37 Rahman CV Ben-David D Dhillon A Kuhn G Gould TWA Muumlller R Rose FRAJ Shakeshecrarr KMLivne E Controlled release of BMP-2 from a sintered polymer scacrarrold enhances bone repair in a mousecalvarial defect model J Tissue Eng Regen Med 2014 8 59ndash66 [CrossRef]
38 Pakulska MM Elliott Donaghue I Obermeyer JM Tuladhar a McLaughlin CK Shendruk TNShoichet MS Encapsulation-free controlled release Electrostatic adsorption eliminates the need for proteinencapsulation in PLGA nanoparticles Sci Adv 2016 2 e1600519 [CrossRef]
39 Fu C Yang X Tan S Song L Enhancing Cell Proliferation and Osteogenic Dicrarrerentiation of MC3T3-E1Pre-osteoblasts by BMP-2 Delivery in Graphene Oxide-Incorporated PLGAHA Biodegradable MicrocarriersSci Rep 2017 7 12549 [CrossRef]
40 Peula JM de las Nieves FJ Adsorption of monomeric bovine serum albumin on sulfonated polystyrenemodel colloids 1 Adsorption isotherms and ecrarrect of the surface charge density Colloids Surf A PhysicochemEng Asp 1993 77 199ndash208 [CrossRef]
41 Peula JM de las Nieves FJ Adsorption of monomeric bovine serum albumin on sulfonated polystyrenemodel colloids 3 Colloidal stability of latexmdashProtein complexes Colloids Surf A Physicochem Eng Asp1994 90 55ndash62 [CrossRef]
42 Peula JM Hidalgo-Alvarez R De Las Nieves FJ Coadsorption of IgG and BSA onto sulfonated polystyrenelatex I Sequential and competitive coadsorption isotherms J Biomater Sci Polym Ed 1996 7 231ndash240[CrossRef]
43 Siafaka PI Uumlstuumlndag Okur N Karavas E Bikiaris DN Surface modified multifunctional and stimuliresponsive nanoparticles for drug targeting Current status and uses Int J Mol Sci 2016 17 1440[CrossRef]
44 Peula-Garciacutea JM Hidalgo-Alvarez R De Las Nieves FJ Colloid stability and electrokinetic characterizationof polymer colloids prepared by dicrarrerent methods Colloids Surf A Physicochem Eng Asp 1997 127 19ndash24[CrossRef]
45 Santander-Ortega MJ Lozano-Loacutepez MV Bastos-Gonzaacutelez D Peula-Garciacutea JM Ortega-Vinuesa JLNovel core-shell lipid-chitosan and lipid-poloxamer nanocapsules Stability by hydration forces ColloidPolym Sci 2010 288 159ndash172 [CrossRef]
46 Peula-Garcia JM Hidaldo-Alvarez R De las Nieves FJ Protein co-adsorption on dicrarrerent polystyrenelatexes Electrokinetic characterization and colloidal stability Colloid Polym Sci 1997 275 198ndash202[CrossRef]
47 Santander-Ortega MJ Bastos-Gonzaacutelez D Ortega-Vinuesa JL Electrophoretic mobility and colloidalstability of PLGA particles coated with IgG Colloids Surf B Biointerfaces 2007 60 80ndash88 [CrossRef]
48 Peula JM Callejas J de las NIeves FJ Adsorption of Monomeric Bovine Serum Albumin on SulfonatedPolystyrene Model Colloids II Electrokinetic Characterization of Latex-Protein Complexes In SurfaceProperties of Biomaterials Butterworth and Heinemann Oxford UK 1994 pp 61ndash69
49 Sun D Ecrarrect of Zeta Potential and Particle Size on the Stability of SiO2 Nanospheres as Carrier for UltrasoundImaging Contrast Agents Int J Electrochem Sci 2016 8520ndash8529 [CrossRef]
50 del Castillo-Santaella T Peula-Garciacutea JM Maldonado-Valderrama J Joacutedar-Reyes AB Interaction ofsurfactant and protein at the OW interface and its ecrarrect on colloidal and biological properties of polymericnanocarriers Colloids Surf B Biointerfaces 2019 173 295ndash302 [CrossRef]
51 Schrier JA DeLuca PP Porous bone morphogenetic protein-2 microspheres Polymer binding and in vitrorelease AAPS PharmSciTech 2001 2 66ndash72 [CrossRef]
52 Padial-Molina M OrsquoValle F Lanis A Mesa F Dohan Ehrenfest DM Wang H-L Galindo-Moreno PClinical application of mesenchymal stem cells and novel supportive therapies for oral bone regenerationBioMed Res Int 2015 2015 [CrossRef]
53 Inai K Norris RA Hocrarrman S Markwald RR Sugi Y BMP-2 induces cell migration and periostinexpression during atrioventricular valvulogenesis Dev Biol 2008 315 383ndash396 [CrossRef]
54 Gamell C Osses N Bartrons R Ruumlckle T Camps M Rosa JL Ventura F Imamura T BMP2 inductionof actin cytoskeleton reorganization and cell migration requires PI3-kinase and Cdc42 activity J Cell Sci2008 121 3960ndash3970 [CrossRef]
55 Friedrichs M Wirsdoumlerfer F Floheacute SB Schneider S Wuelling M Vortkamp A BMP signaling balancesproliferation and dicrarrerentiation of muscle satellite cell descendants BMC Cell Biol 2011 12 26 [CrossRef]
Pharmaceutics 2019 11 388 18 of 18
56 Hrubi E Imre L Robaszkiewicz A Viraacuteg L Kereacutenyi F Nagy K Varga G Jenei A Hegeduumls CDiverse ecrarrect of BMP-2 homodimer on mesenchymal progenitors of dicrarrerent origin Hum Cell 2018 31139ndash148 [CrossRef]
57 Kim HKW Oxendine I Kamiya N High-concentration of BMP2 reduces cell proliferation and increasesapoptosis via DKK1 and SOST in human primary periosteal cells Bone 2013 54 141ndash150 [CrossRef]
copy 2019 by the authors Licensee MDPI Basel Switzerland This article is an open accessarticle distributed under the terms and conditions of the Creative Commons Attribution(CC BY) license (httpcreativecommonsorglicensesby40)
157
158
12ANEXO DE ORIGINALIDAD
Editor Universidad de Granada Tesis Doctorales Autor Inmaculada Ortega Oller ISBN 978-84-1306-879-4
URI httphdlhandlenet1048168568
2
3
BIO-NANOTECNOLOGIacuteA APLICADA A LA
REGENERACIOacuteN OacuteSEA MEDIANTE EL
TRANSPORTE DE BIOMOLEacuteCULAS USANDO
NANOPARTIacuteCULAS POLIMEacuteRICAS ESTUDIO
IN VITRO
por
Inmaculada Ortega Oller
Licenciada en Odontologiacutea
Directores de la Tesis
Dr D Joseacute Manuel Peula
Prof Titular de Fiacutesica Aplicada
Dr D Francisco OacuteValle Ravassa
Prof Catedraacutetico de Anatomiacutea Patoloacutegica
Dr D Pablo Galindo Moreno
Prof Catedraacutetico de Estomatologiacutea
4
A mi familia
y directores y en especial
a mi PADRE
Juan Ortega Navarro
5
Agradecimientos
Despueacutes de un apasionado y largo periacuteodo de elaboracioacuten de esta tesis doctoral hoy es el diacutea
escribo este apartado de agradecimientos para finalizar un arduo trabajo Ha sido un periacuteodo de
aprendizaje intenso no solo en el campo cientiacutefico sino tambieacuten a nivel personal que ha supuesto
un gran impacto en miacute Por tal motivo me gustariacutea agradecer a todas aquellas personas que me
han ayudado y brindado su apoyado durante este proceso
En primer lugar quiero agradecer a mis tutores
Don Jose Manuel Peula Garciacutea Profesor Titular de Fiacutesica Aplicada quien con sus
conocimientos y apoyo me guioacute a traveacutes de cada una de las etapas de este proyecto para alcanzar
los resultados que buscaba Gracias por su paciencia calma y tranquilidad para explicar
detenidamente a una odontoacuteloga todos y cada uno de los conceptos aprendidos e interiorizados
Todo mi agradecimiento por su tiempo dedicacioacuten y por haberme acogido de la manera que lo
hizo daacutendome todo su carintildeo apoyo y compresioacuten
Don Pablo Galindo Moreno Profesor Catedraacutetico de Estomatologiacutea
Un trabajo de investigacioacuten es siempre fruto de ideas proyectos y esfuerzos previos que
corresponden a otras personas y que te escogen a ti para saber llevarlas a cabo En este caso mi
maacutes sincero agradecimiento a usted con cuyo trabajo estareacute siempre en deuda y con quien he
compartido proyectos e ilusiones durante todos estos antildeos Gracias por su amabilidad para
facilitarme su tiempo y sus ideas Ha sido una fuente de paz en tiempos muy duros
6
Don Francisco OacuteValle Ravassa Profesor Catedraacutetico de Anatomiacutea Patoloacutegica por su
orientacioacuten atencioacuten a mis consultas y por sus valiosas sugerencias en momentos de duda asiacute
como por su completa disponibilidad siempre que lo he necesitado
Tambien quiero agradecer a Mis Iberica SL y a la Consejeriacutea de Economiacutea Innovacioacuten
Educacioacuten Ciencia y Empleo de la Junta de Andaluciacutea por brindarme todos los recursos y
herramientas que fueron necesarios para llevar a cabo el proceso de investigacioacuten No hubiese
podido arribar a estos resultados sin su incondicional ayuda
Un trabajo de investigacioacuten es tambieacuten fruto del reconocimiento y del apoyo vital que nos
ofrecen las personas que nos estiman sin el cual no tendriacuteamos la fuerza y energiacutea que nos anima
a crecer como personas y como profesionales este es el caso del Dr Don Miguel Padial y de las
Dras Dontildea Azahara Rata-Aguilar Dontildea Ana Jodar y DontildeaTeresa Del Castillo Gracias por
vuestra cercana visioacuten de todo Tambien queriacutea dedicar unas palabras a un buen amigo Javier
Vidao quien con sus conocimientos informaacuteticos me ha ayudado con la elaboracioacuten de este
documento de tesis Gracias
Quiero agradecer tambieacuten a todos mis compantildeeros y amigos por hacer feliz mi dia a dia y a mi
familia por apoyarme auacuten cuando mis aacutenimos decaiacutean A mis hermanos Juan Javier y Dorothy y
a mi madre que siempre estuvieron ahiacute para darme palabras de apoyo y un abrazo reconfortante
para renovar energiacuteas
Gracias a mi pareja por su paciencia comprensioacuten y solidaridad con este proyecto por el
tiempo que me ha concedido un tiempo robado al disfrute conjunto que ha respetado y valorado
Te agradezco la esperanza que me has brindado en los momentos y situaciones mas tormentosas
de mi vida
7
Por uacuteltimo dedico con todo mi corazoacuten mi tesis doctoral a mi PADRE a quien perdimos
recientemente pues sin eacutel no habriacutea logrado nada de esto gracias por haberme forjado como la
persona que hoy soy
Muchos de mis logros se los debo a eacutel quien me educoacute con firmeza pero a la vez sabiendo
darme la libertad suficiente para permitirme evolucionar por mi misma como persona
motivandome constantemente a alcanzar mis metas Fue mi referente en la vida y quien me dioacute el
uacuteltimo y maacutes grande de los aprendizajes dejando en miacute un gran espiacuteritu de lucha sacrificio
esfuerzo y sabiduriacutea ante la maacutes difiacutecil situacioacuten planteable Por todo ello este trabajo te lo dedico
a ti alliacute donde esteacutes espero que seas feliz y puedas disfrutar de los logros conseguidos aquiacute
A todos muchas gracias
8
RESUMEN
REGENERACIOacuteN OacuteSEA A PARTIR DE NANOMICROPARTICULAS DE PLGA
CARGADAS DE BMP-2
El aacutecido poli-laacutectico-co-glicoacutelico (PLGA) es uno de los poliacutemeros sinteacuteticos maacutes ampliamente
utilizados para el desarrollo de sistemas de administracioacuten de faacutermacos y biomoleacuteculas
terapeacuteuticas asi como componente principal en aplicaciones de ingenieriacutea de tejidos Sus
propiedades y versatilidad le permiten ser un poliacutemero de referencia en la fabricacioacuten de
nanopartiacuteculas y micropartiacuteculas para encapsular y liberar una amplia variedad de moleacuteculas
hidrofoacutebicas e hidrofiacutelicas Ademaacutes sus propiedades de biodegradabilidad y biocompatibilidad
hacen del mismo un candidato idoacuteneo para encapsular biomoleacuteculas como proteiacutenas o aacutecidos
nucleicos permitiendo su liberacioacuten de forma controlada
Este trabajo se centra en el uso de nanopartiacuteculas (NP) de PLGA como un sistema de entrega
de uno de los factores de crecimiento maacutes comuacutenmente utilizados en la ingenieriacutea del tejido oacuteseo
la proteiacutena morfogeneacutetica oacutesea 2 (BMP2) Por lo tanto examinamos todos los requisitos
necesarios para alcanzar una correcta encapsulacioacuten y una liberacioacuten controlada y sostenida de
BMP2 utilizando partiacuteculas de PLGA como componente principal discutiendo todos los
problemas y soluciones que hemos encontrado para el desarrollo adecuado de este sistema con un
gran potencial en el proceso de diferenciacioacuten celular y proliferacioacuten bajo el punto de vista de la
regeneracioacuten oacutesea
Hemos desarrollado y optimizando dos meacutetodos de formulacioacuten diferentes para obtener NP de
PLGA cargadas con una proteiacutena modelo con actividad enzimaacutetica como la lisozima que posee
caracteriacutesticas similares a la BMP2 Estas formulaciones se basan en una teacutecnica de doble
emulsioacuten con evaporacioacuten de solvente (agua aceite agua WOW) Se diferencian
principalmente en la fase en la que se agrega el surfactante (Pluronicreg F68) agua (W-F68) o
9
aceite (O-F68) Este surfactante polimeacuterico no ioacutenico puede modular una serie de propiedades del
nanosistema transportador en el que se integra reduciendo el tamantildeo de las NPs incrementando
su estabilidad coloidal y facilitando la proteccioacuten de la biomoleacutecula encapsulada Ademaacutes gracias
a su disposicioacuten superficial y la hidrofilidad de sus colas polares se reduce la interaccioacuten con el
sistema fagociacutetico mononuclear con una mejora de la biodistribucioacuten al aumentar su tiempo de
circulacioacuten despueacutes de una administracioacuten intravenosa en un organismo vivo
Analizamos las propiedades coloidales de estos sistemas usando diferentes teacutecnicas
experimentales (morfologiacutea por SEM y STEM tamantildeo hidrodinaacutemico por DLS y NTA
movilidad electroforeacutetica estabilidad temporal en diferentes medios) asiacute como la encapsulacioacuten
patroacuten de liberacioacuten y bioactividad de la lisozima Asimismo realizamos una caracterizacioacuten
interfacial de la interaccioacuten surfactante-proteiacutena en la primera emulsioacuten agua-aceite para cada
procedimiento de formulacioacuten mediante el anaacutelisis de la tensioacuten superficial y la elasticidad
Finalmente examinamos la captacioacuten celular por ceacutelulas estromales mesenquimaacuteticas humanas y
la citotoxicidad para ambos nanosistemas
Mediante las dos formulaciones O-F68 y W-F68 se obtienen NPs soacutelidas de morfologiacutea
esfeacuterica si bien en un caso el sistema presenta monodispersidad con diaacutemetros alrededor de 120
nm (O-F68) en el otro se obtiene un nanosistema polidisperso con diaacutemetros de partiacutecula
comprendidos entre 100 y 500 nm (W-F68) Como resultado maacutes relevante observamos que la
eficacia de encapsulacioacuten la liberacioacuten y la bioactividad de la lisozima se han mantenido mejor
con el meacutetodo de formulacioacuten W-F68 En este caso dada la heterogeneidad de tamantildeos se podriacutea
hablar de un prometedor sistema multimodal para encapsular proteiacutenas con una fuerte actividad
bioloacutegica que permita una ldquoentrega dualrdquo a nivel extra- e intracelular facilitando la actividad
proteica en la superficie celular y en el citoplasma
Tras desarrollar y optimizar el meacutetodo de siacutentesis para las NPs de PLGA cargadas de lisozima
tratamos de adaptar la formulacioacuten para conseguir la encapsulacioacuten de la proteiacutena terapeacuteutica
BMP-2 Asiacute basaacutendonos en los resultados obtenidos con la lisozima se ha optado por usar el
10
procedimento de siacutentesis W-F68 para favorecer la proteccioacuten de las moleacuteculas proteicas y su
actividad bioloacutegica Con esta formulacioacuten se han obtenido con buena reproducibilidad NPs
esfeacutericas con el tamantildeo multimodal referido anteriormente entre 100 y 500 nm que posibilitaraacuten
el suministro extra- e intracelular Ademaacutes de NPs con BMP2 encapsulada obtenemos un
nanosistema en el que la BMP2 no estaacute encapsulada sino co-adsorbida superficialmente junto a
una proteiacutena estabilizadora como la albuacutemina de suero bovino De nuevo se lleva a cabo una
completa caracterizacioacuten fisico-quiacutemica y bioloacutegica de ambos sistemas de NPs analizando las
propiedades indicadas previamente esto es morfologiacutea y tamantildeo carga superficial estabilidad
coloidal y temporal encapsulacioacuten y patroacuten de liberacioacuten Es conocido que la cineacutetica de
liberacioacuten en los sistemas polimeacutericos basados en PLGA dependen en gran medida de la
degradacioacuten hidroliacutetica del poliacutemero Sin embargo la liberacioacuten a tiempos cortos estaacute influenciada
por otros procesos fiacutesicos y es crucial evitar una descarga inicial excesiva sobre todo si se quiere
optimizar la aplicacioacuten de esta nanotecnologiacutea en procesos de regeneracioacuten oacutesea muy importantes
en odontologiacutea En consecuencia hemos incidido en el anaacutelisis del patroacuten de liberacioacuten de la
BMP2 a tiempos cortos utilizando diferentes teacutecnicas y comparando el comportamiento de los dos
sistemas de NPs con la proteiacutena encapsulada o adsorbida superficialmente
Finalmente se ha analizado la actividad bioloacutegica de las NPs cargadas con BMP2 mediante
estudios in vitro de proliferacioacuten celular migracioacuten y diferenciacioacuten osteogeacutenica usando para ello
ceacutelulas estromales mesenquimales obtenidas a partir de hueso alveolar humano (ABSC) En base
a todo esto se puede confirmar que las NPs con BMP2 encapsuladas presentan un patroacuten de
liberacioacuten adecuado a corto plazo manteniendo un suministro proteico sostenido y una actividad
bioloacutegica adecuada para dosis iniciales de BMP2 muy reducidas
11
SUMMARY
BONE REGENERATION FROM PLGA NANOMICROPARTICLES LOADED
WITH BMP-2
Poly-lactic-co-glycolic acid (PLGA) is one of the most widely used synthetic polymers for the
development of drug delivery systems and therapeutic biomolecules and as a component of tissue
engineering applications Its properties and versatility allow it to be a reference polymer in the
manufacture of nanoparticles and microparticles to encapsulate and release a wide variety of
hydrophobic and hydrophilic molecules Furthermore its biodegradability and biocompatibility
properties make it an ideal candidate for encapsulating biomolecules such as proteins or nucleic
acids that can be released in a controlled manner This work focuses on the use of PLGA
nanoparticles (NP) as a delivery system for one of the most commonly used growth factors in
bone tissue engineering bone morphogenetic protein 2 (BMP2) Therefore we examine all the
necessary requirements to achieve a correct encapsulation and a controlled and sustained release
of BMP2 using PLGA particles as the main component discussing all the problems and solutions
that we have found for the proper development of this system with great potential in the process
of cell differentiation and proliferation from the point of view of bone regeneration We have
developed and optimized two different formulation methods to obtain PLGA NP loaded with a
model protein with enzymatic activity such as lysozyme with similar characteristics to BMP2
These formulations are based on a double emulsion technique with solvent evaporation
(wateroilwater WO W) They differ mainly in the phase in which the surfactant (Pluronicreg
F68) is added water (W-F68) or oil (O-F68) This non-ionic polymeric surfactant can modulate
a series of properties of the transporter nanosystem in which it is integrated reducing the size of
the NPs increasing their colloidal stability and facilitating the protection of the encapsulated
biomolecule Furthermore thanks to its superficial arrangement and the hydrophilicity of its polar
12
tails interaction with the mononuclear phagocytic system is reduced with an improvement in
biodistribution by increasing its circulation time after intravenous administration in a living
organism The colloidal properties of these systems have been analyzed using different
experimental techniques (morphology by SEM and STEM hydrodynamic size by DLS and NTA
electrophoretic mobility temporal stability in different media as well as the encapsulation
release pattern and bioactivity of the lysozyme Likewise an interfacial characterization of the
surfactant-protein interaction was carried out in the first water-oil emulsion for each formulation
procedure by analyzing the surface tension and elasticity Finally we analyzed the cellular uptake
by human mesenchymal stromal cells and cytotoxicity for both nanosystems Through the two
formulations O-F68 and W-F68 solid NPs of spherical morphology are obtained although in
one case the system presents monodispersity with diameters around 120 nm (O-F68) while in
the other a Polydisperse nanosystem with particle diameters between 100 and 500 nm (W-F68)
As a more relevant result we observed that the encapsulation efficiency the release and the
bioactivity of lysozyme have been better maintained with the W-F68 formulation method In this
case given the heterogeneity of sizes one could speak of a promising multimodal system to
encapsulate proteins with strong biological activity that allows a dual delivery at the extra- and
intracellular level facilitating protein activity on the cell surface and in the cytoplasm After
developing and optimizing the synthesis method for lysozyme-loaded PLGA NPs we tried to
adapt the formulation to achieve encapsulation of the therapeutic protein BMP-2 Thus based on
the results obtained with lysozyme it was decided to use the W-F68 synthesis procedure to favor
the protection of protein molecules and their biological activity With this formulation spherical
NPs with the aforementioned multimodal size between 100 and 500 nm have been obtained with
good reproducibility which would allow extra- and intracellular delivery In addition to NPs with
encapsulated BMP2 a nanosystem has been obtained in which BMP2 is not encapsulated but is
superficially co-adsorbed with a stabilizing protein such as bovine serum albumin Again a
complete physico-chemical and biological characterization of both NPs systems is carried out
13
analyzing the previously indicated properties that is morphology and size surface charge
colloidal and temporal stability encapsulation and release pattern
It is known that release kinetics in PLGA-based polymer systems are highly dependent on the
hydrolytic degradation of the polymer However the short-time release is influenced by other
physical processes and it is crucial to avoid an excessive initial discharge especially if the
application of this nanotechnology is to be optimized in very important bone regeneration
processes in dentistry Consequently we have focused on the analysis of the release pattern of
BMP2 at short times using different techniques and comparing the behavior of the two NPs
systems with the encapsulated or superficially adsorbed protein Finally the biological activity of
NPs loaded with BMP2 has been analyzed by in vitro studies of cell proliferation migration and
osteogenic differentiation using mesenchymal stromal cells obtained from human alveolar bone
(ABSC) Based on all this it can be confirmed that NPs with encapsulated BMP2 present an
adequate release pattern in the short term maintaining a sustained protein supply and adequate
biological activity for very low initial doses of BMP2
14
LISTA DE PUBLICACIONES
1 Ortega-Oller I Padial-Molina M Galindo-Moreno P OrsquoValle F Joacutedar-Reyes A B
Peula-Garciacutea J M Bone Regeneration from PLGA Micro-Nanoparticles Biomed Res
Int 2015 vol 2015 1ndash18 doi1011552015415289 IF Q2 Rank 82161 JIFpercentil
494 Nordm de citas 33
2 Ortega-Oller I del Castillo-Santaella T Padial-Molina M Galindo-Moreno P Joacutedar-
Reyes A B Peula-Garciacutea J M Dual delivery nanosystem for biomolecules
Formulation characterization and in vitro release Colloids Surfaces B Biointerfaces
2017 159 586ndash595doi 101016jcolsurfb201708027 IF Q1 Rank1272 JIF
percentil 826 Nordmcitas 5
3 del Castillo-Santaella T Ortega-Oller I Padial-Molina M OrsquoValle F Galindo-
Moreno P Joacutedar-Reyes A B Peula-Garciacutea J M Formulation Colloidal
Characterization and In Vitro Biological Effect of BMP-2 Loaded PLGA Nanoparticles
for Bone Regeneration Pharmaceutics 2019 11(8) 388
doi103390pharmaceutics11080388 IF Q1 Rank26267 JIFpercentil 905 Nordmcitas
3
15
16
Iacutendice
0 GLOSARIO (lista de abreviaturas) helliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphellip 19
1 INTRODUCCIOacuteN 23
11 BMPS ACCIOacuteN Y REGULACIOacuteN 24
111 Uso cliacutenico de la BMP-2 27
12 PARTIacuteCULAS COLOIDALES POLIMEacuteRICAS PARA ENCAPSULAR MOLEacuteCULAS HIDROFIacuteLICAS 30
121Meacutetodos de siacutentesis 33
123 Tamantildeo y morfologiacutea de las partiacuteculas 35
13AGENTES ESTABILIZADORES 39
131 Estabilidad coloidal 39
132 Eficacia de encapsulacioacuten y bioactividad 42
14 PATROacuteN DE LIBERACIOacuteN 44
15 VECTORIZACIOacuteN ENTREGA DIRIGIDA 52
16 INGENIERIacuteA TISULAR SOPORTES 3D O ldquoSCAFFOLDSrdquo 53
2 HIPOacuteTESIS 55
3 OBJETIVOS 56
31 OBJETIVO PRINCIPAL 56
32 OBJETIVOS SECUNDARIOS 56
4 NANOSISTEMA DE ENTREGA DOBLE PARA BIOMOLEacuteCULAS FORMULACIOacuteN CARACTERIZACIOacuteN
Y LIBERACIOacuteN IN VITRO 58
41 ANTECEDENTES 58
42 MATERIALES Y MEacuteTODOS 60
421Formulacioacuten de las nanoparticulas 60
422 Limpieza y almacenamiento 61
423 Caracterizacioacuten de las nanoparticulas 62
424 Estabilidad coloidal y temporal en biologiacutea media 63
425 Actividad bioloacutegica e interacciones 64
43 RESULTADOS Y DISCUSIOacuteN 65
17
431 Formulacioacuten de las nanoparticulas 65
432 Caracterizacioacuten de las Nanopartiacuteculas 69
433 Actividad bioloacutegica e interacciones 84
5 FORMULACIOacuteN CARACTERIZACIOacuteN COLOIDAL Y EFECTO BIOLOacuteGICO IN VITRO DE
NANOPARTIacuteCULAS DE PLGA CARGADAS CON BMP-2 PARA LA REGENERACIOacuteN OacuteSEA 96
51 ANTECEDENTES 96
52 MATERIALES Y MEacuteTODOS 98
521Siacutentesis de nanoparticulas 98
522 Caracterizacioacuten de nanopartiacuteculas morfologiacutea tamantildeo concentracioacuten y movilidad electrocineacutetica
101
523 Estabilidad coloidal y temporal en medios bioloacutegicos 101
524 Interacciones celulares 102
53 RESULTADOS Y DISCUSIOacuteN 105
531Formulacioacuten de nanoparticulas 105
532Caracterizacioacuten de nanopartiacuteculas 109
533Actividad bioloacutegica e interacciones 118
6 CONCLUSIONES 125
7 CONFLICTO DE INTERESES 127
8 RECURSOS ECONOacuteMICOS 127
9 BIBLIOGRAFIacuteA 128
10 ANEXO MATERIAL SUPLEMENTARIO 154
- Enlace a videos
11 ANEXO DE PUBLICACIONES 156
-Artiacuteculo 1 Bone regeneration from PLGA Micro-Nanoparticles
-Artiacuteculo 2 Dual delivery nanosystem for biomolecules Formulation characterization and
in vitro release
18
-Artiacuteculo 3 Formulation Colloidal Characterization and In Vitro Biological Effect of
BMP-2 Loaded PLGA Nanoparticles for Bone Regeneration
12 ANEXO DE ORIGINALIDAD 158
19
LISTA DE ABREVIACIONES
NP Nanopartiacuteculas
MP Micropartiacuteculas
MSC Ceacutelulas mesenquimales
BMP Proteiacutena morfogeneacutetica oacutesea
Rh BMP Proteiacutena morfogeneacutetica oacutesea recombinante
BMP I y II Proteiacutena morfogeneacutetica oacutesea receptora I y II
GF Factor de crecimiento
PDGF Factor de crecimiento derivado de plaquetas
FGF Factor de crecimiento de fibroblastos
IGF Factor de crecimiento de insulina
TGF- Factor de crecimiento transformante
RUNX2 Factor de transcripcioacuten
MPS Sistema fagociacutetico mononuclear
hMSC Ceacutelulas mesenquimales humanas
ABSC Ceacutelulas estromales mesenquimales oacutesea
OSX Osterix
LMP Proteiacutena de mineralizacioacuten de dominio Lim
SEM Microscopia electroacutenica de barrido
STEM Microscopia electroacutenica de transmisioacuten de barrido
PLA Aacutecido poli-lactico
PLGA Aacutecido poli-lactico co-glicolico
LYS Lisozima
LYSF Lisozima final (encapsulada)
20
F68 Pluronic (W-F68= en agua) (O-F68= en aceite)
WOW Doble emulsioacuten de agua en aceite
AP Fase acuosa
OP Fase orgaacutenica
SA Albuacutemina seacuterica
BSA Albuacutemina seacuterica bovina
HSA Albuacutemina seacuterica humana
PVA Alcohol poliviniacutelico
PBS Tampoacuten fosfato salino
PB Tampoacuten fosfato
FBS Suero fetal bovino
PEO Oacutexido de polietileno
DCM Diclorometano
EA Acetato de etilo
FITC Isotiocianato de fluoresceiacutena
DPPC Dipalmitoil-fosfatidilcolina
PGE Polietilenglicol
DC Aacutecido dexosicoacutelico
BCA Aacutecido bicinconiacutenico
DTT Ditiotreitol
SDS Duodecil sulfato de sodio
ALP Fosfatasa alcalina
GAPDH Gliceraldehiacutedo-3-fosfato deshidrogenasa
SDS Gel duodecilsulfato de sodio
PI3K Fosfoinositida 3-quinasa
ALP Fosfatasa alcalina
21
SDS-PAGE Electroforesis en gel poliacrilamida con duodecilsulfato soacutedico
PDI Iacutendice de polidispersidad
DLS Dispersioacuten de luz dinaacutemica
EE Eficacia de encapsulacioacuten
SRB Absorbancia de sulforamida
DL Carga del faacutermaco
FDA Administracioacuten de medicamentos y alimentos
RMN Resonancia magneacutetica nuclear
NTA Anaacutelisis de seguimiento de nanopartiacuteculas
NLS Sentildeales de localizacioacuten nuclear
DMEM Medio Eagle modificado con dulbecco
23
1 INTRODUCCIOacuteN
La regeneracioacuten oacutesea es uno de los principales desafiacuteos a los que nos enfrentamos en la cliacutenica
diariamente Inmediatamente despueacutes de la extraccioacuten de un diente los procesos bioloacutegicos
normales remodelan el hueso alveolar limitando en algunos casos la posibilidad de una futura
colocacioacuten de implante En los uacuteltimos antildeos han sido estudiadas diferentes estrategias para llevar
a cabo la preservacioacuten de ese hueso Otras afecciones como el traumatismo la cirugiacutea de
reseccioacuten tumoral o las deformidades congeacutenitas requieren requisitos teacutecnicos y bioloacutegicos auacuten
mayores para generar la estructura oacutesea necesaria para la rehabilitacioacuten oclusal del paciente Para
superar estas limitaciones anatoacutemicas en teacuterminos de volumen oacuteseo existen diferentes enfoques
para mejorar la osteointegracioacuten del implante o para aumentar la anatomiacutea del hueso donde se
colocaraacute el futuro implante (M Padial-Molina P Galindo-Moreno 2009) (Al-Nawas and
Schiegnitz 2014) El injerto oacuteseo autoacutegeno todaviacutea se considera el ldquogold estaacutendarrdquo debido a sus
propiedades osteogeacutenicas osteoconductivas y osteoinductivas (Katranji Fotek and Wang 2008)
(Misch 1987) Sin embargo tambieacuten presenta varias limitaciones incluida la necesidad de una
segunda cirugiacutea disponibilidad limitada y morbilidad en el aacuterea donante (Myeroff and
Archdeacon 2011) Por lo tanto otros biomateriales como injertos alogeacutenicos e injertos
xenogeacutenicos con osteoconductividad y capacidades osteoinductivas (Avila et al 2010) (Froum
et al 2006) fueron propuestos (Galindo-Moreno et al 2007) (Galindo-Moreno et al 2011) asiacute
como biomateriales aloplaacutesticos (Wheeler 1997) con potencial osteoconductivo Todos estos
materiales aunque aceptables no son adecuados en muchas condiciones y generalmente requieren
una consideracioacuten adicional en el proceso de decisioacuten (Wallace and Froum 2003) Ademaacutes la
cantidad y calidad de hueso que se puede obtener con estos materiales a menudo es limitada
El uso de moleacuteculas bioactivas por siacute solas o en combinacioacuten con los materiales descritos
previamente se ha convertido por lo tanto en un aacuterea de intereacutes principal gracias a su alto
potencial Al usar este tipo de procedimientos es importante considerar 1) el meacutetodo de
administracioacuten y 2) la moleacutecula por siacute misma Las moleacuteculas bioactivas pueden transportarse al
24
aacuterea del defecto como una solucioacuten o un gel incrustados en esponjas adheridos a scaffolds soacutelidos
y maacutes recientemente incluidos en partiacuteculas de diferentes tamantildeos Usando estos meacutetodos se
puede acudir a una gran diversidad de biomoleacuteculas como PDGF (factor de crecimiento
derivado de plaquetas) FGF (factor de crecimiento de fibroblastos) IGF (factor de crecimiento
de insulina) RUNX2 osterix (Osx) proteiacutena de mineralizacioacuten de dominio LIM (LMP) BMP
(proteiacutena morfogeacutenica oacutesea) y maacutes recientemente periostin como candidatos potenciales para los
procedimientos de regeneracioacuten dentro de la cavidad oral incluidos los tejidos oacuteseos y
periodontales (Padial-Molina and Rios 2014) (Padial-Molina Volk and Rios 2014) Estas
moleacuteculas se probaron solas o en combinacioacuten con ceacutelulas madre (Behnia et al 2012) utilizando
varias estrategias in vitro e in vivo (Padial-Molina et al 2012)
11 BMPs Accioacuten y regulacioacuten
En regeneracioacuten oacutesea y en particular los factores de crecimiento morfogeneacuteticos oacuteseos (BMP)
son probablemente el grupo de moleacuteculas maacutes comuacuten Desde 1965 cuando Urist (Urist 1965)
demostroacute que las BMPs oacuteseas extraiacutedas podriacutean inducir la formacioacuten de hueso y cartiacutelago cuando
se implantan en tejido animal un alto nuacutemero de artiacuteculos han probado su aplicacioacuten in vivo y su
base bioloacutegica cuando se usan en defectos oacuteseos (Boyne and Jones 2004) (Wang et al 1990)
(Wozney 1992) Las BMPs son miembros de la suacuteper familia de proteiacutenas TGF-β (Barboza
Caula and Machado 1999) La familia de proteiacutenas BMP agrupa maacutes de 20 proteiacutenas
morfogeneacuteticas homodimeacutericas o heterodimeacutericas que funcionan en muchos tipos y tejidos
celulares no todos tienen que ser necesariamente osteogeacutenicos (Ana Claudia Carreira et al
2014) Las BMPs se pueden dividir en 4 subfamilias seguacuten su funcioacuten y secuencia siendo BMP-
2 -4 y -7 las que tienen un fuerte potencial osteogeacutenico (Ana Claudia Carreira et al 2014) Las
acciones de las BMPs incluyen la condrogeacutenesis la osteogeacutenesis la angiogeacutenesis y la siacutentesis de
la matriz extracelular (Bustos-Valenzuela et al 2011) Dentro de esta familia de proteiacutenas BMP-
2 ha sido la maacutes estudiada Tiene propiedades osteoinductoras que promueven la formacioacuten de
25
nuevo hueso al iniciar estimular y amplificar la cascada de la formacioacuten oacutesea a traveacutes de la
quimiotaxis y la estimulacioacuten de la proliferacioacuten y diferenciacioacuten del linaje celular osteoblaacutestico
(Myeroff and Archdeacon 2011) (Boyne and Jones 2004) (Wozney 1992) (Barboza Caula and
Machado 1999) La ausencia de eacutesta como se estudioacute en los modelos eliminatorios conduce a
fracturas espontaacuteneas que no cicatrizan con el tiempo (Tsuji et al 2006) De hecho otros modelos
han demostrado que la ausencia de cualquiera de estas dos BMP-4 (Tsuji et al 2008) o -7 (Tsuji
et al 2010) no conducen a la formacioacuten de hueso y deterioro como demuestra el efecto producido
por BMP-2 sola (Chen Deng and Li 2012)
Muchos tipos de ceacutelulas en el tejido oacuteseo producen BMP como las ceacutelulas osteoprogenitoras
osteoblastos condrocitos plaquetas y ceacutelulas endoteliales Esta BMP secretada se almacena en la
matriz extracelular donde interactuacutea principalmente con el colaacutegeno tipo IV (Ramel and Hill
2012) Durante los procesos de reparacioacuten y remodelacioacuten la actividad absorbente de los
osteoclastos induce la liberacioacuten de BMP al medio para que se suspenda la funcioacuten de absorcioacuten
y eacutesta pueda interactuar con las ceacutelulas cercanas para iniciar el consecuente proceso osteogeacutenico
(A C Carreira et al 2014)
La BMP en la matriz extracelular se une a los receptores de la superficie celular BMPR-I y II
y activa las proteiacutenas citoplasmaacuteticas Smad o la viacutea MAPK (Deschaseaux Sensebe and Heymann
2009) Cuando BMPR-I se activa BMPR-II se engancha y se activa tambieacuten (Mueller and Nickel
2012) La activacioacuten del complejo BMPR-I y BMPR-II conduce a la activacioacuten de varios Smads
(1 5 y 8) que tambieacuten activan Smad-4 y todos forman complejos proteicos que se transportan al
nuacutecleo donde Runx2 Dlx5 y los genes Osterix (importantes en la osteogeacutenesis) se activan (Chen
Deng and Li 2012) (Ramel and Hill 2012) (Figura 1) De forma similar cuando se activa la ruta
de MAPK conduce a la induccioacuten de la transcripcioacuten de Runx2 y por lo tanto a la diferenciacioacuten
oacutesea (Sieber et al 2009) Tambieacuten se han descrito varios antagonistas extracelulares e
intracelulares que incluyen noggin chordin y gremlin o Smad-6 -7 y -8b respectivamente
(Sapkota et al 2007)
26
Figura 1 Representacioacuten esquemaacutetica de la ruta molecular principal de BMP a la
osteogeacutenesis Las BMP interactuacutean con los receptores de la superficie celular I y II para activar
Smads 1 5 y 8 Estos Smads activados activan Smad 4 Todos juntos como un complejo de
proteiacutenas activan Runx2 Dlx5 y Osterix
Foto tomada de Articulo Ortega-Oller I Padial-Molina M Galindo-Moreno P OacuteValle F
Jodar-Reyes AB Peula-Garcia JM Bone regeneration form Plga Micro-Nanoparticles BioMed
Research International 2015 415289 (2015)
27
111Uso cliacutenico de la BMP-2
Hoy en diacutea la BMP-2 estaacute disponible comercialmente bajo diferentes nombres de marcas y
concentraciones Por lo general consiste en una esponja absorbible de colaacutegeno fijada con BMP-
2 humana recombinante En 2002 fue aprobado por la FDA como una alternativa de injerto oacuteseo
autoacutegeno en la fusioacuten intersomaacutetica lumbar anterior (McKay Peckham and Badura 2007) Maacutes
tarde en 2007 la FDA aproboacute el uso de rhBMP-2 como una alternativa para el injerto oacuteseo
autoacutegeno en el aumento de los defectos de la cresta alveolar asociados con la extraccioacuten del diente
en la neumatizacioacuten del seno maxilar (McKay Peckham and Badura 2007)
Ademaacutes de las aplicaciones en estudios cliacutenicos de columna donde se usan concentraciones
muy altas (AMPLIFYTM rhBMP-2 40 mg) los estudios cliacutenicos han apoyado su uso en la
cavidad oral Las BMP se han utilizado en la regeneracioacuten periodontal la terapeacuteutica oacutesea la
osteointegracioacuten de implantes la cirugiacutea oral con fines ortodoacutencicos la reparacioacuten de secuelas
derivadas de la patologiacutea oacutesea la osteogeacutenesis por distraccioacuten y la cirugiacutea reparadora de
endodoncia (A C Carreira et al 2014) (Hong et al 2013) Sin embargo han mostrado resultados
maacutes prometedores en casos en los que solo se regeneraraacute el tejido oacuteseo incluido el desarrollo del
sitio pre-implantario la elevacioacuten de seno el aumento de cresta vertical y horizontal y la
cicatrizacioacuten de cirugiacuteas de implantes dentales (Spagnoli and Marx 2011) En este sentido se
evidencioacute que el uso de rhBMP-2 indujo la formacioacuten de hueso adecuado para la colocacioacuten de
implantes dentales y su osteointegracioacuten (Nevins et al 1996) Ademaacutes parece que el hueso recieacuten
formado tiene propiedades similares al hueso nativo y por lo tanto es capaz de soportar las
fuerzas oclusales que ejerce la dentadura durante su funcioacuten masticatoria (Boyne et al 2005)
En resumen los estudios nombrados concluyeron que rhBMP-2 induce la formacioacuten de nuevo
hueso con una calidad y cantidad comparable al inducido por la cicatrizacioacuten del propio paciente
e incluso en algunos de los casos se informoacute de haber obtenido una cantidad y calidad de hueso
mayor a la que se hubiese obtenido por la viacutea de cicatrizacioacuten normal del paciente (Lee et al
2013)
28
Por el contrario estudios recientes revelan graves complicaciones despueacutes de su uso (Ronga et
al 2013) Ademaacutes se han asociado efectos carcinogeacutenicos a altas dosis lo que llevoacute a los autores
a enfatizar en la necesidad de mejores pautas en el uso cliacutenico de BMP (Devine et al 2012) No
tan draacutesticos son los uacuteltimos estudios que destacan los efectos secundarios negativos y los riesgos
de su aplicacioacuten haciendo gran hincapieacute en el sesgo potencial de la investigacioacuten patrocinada por
la industria no reproducible especialmente cuando se utiliza en la meacutedula espinal (Fu et al 2013)
(Carragee Hurwitz and Weiner 2011) (Simmonds et al 2013) Se observoacute tambieacuten que el uso
de rhBMP-2 aumenta el riesgo de complicaciones en la zona tratada disfagia con alta eficacia y
dantildea la tergiversacioacuten mediante informes selectivos publicaciones duplicadas y subregistros (Fu
et al 2013) Especiacuteficamente en el campo de la regeneracioacuten oacutesea dentro de la cavidad oral un
estudio de elevacioacuten de seno concluyoacute que el uso de BMP-2 promueve efectos negativos en la
formacioacuten oacutesea cuando se combina con matriz oacutesea bovina inorgaacutenica vs hueso bovino
inorgaacutenico solo (Kao et al 2012) en contraste con artiacuteculos y revisiones previas (Torrecillas-
Martinez et al 2013) Al tomar en cuenta esta informacioacuten se puede concluir que es de extrema
importancia tener cuidado con el uso cliacutenico de nuevos productos evitando las aplicaciones no
clasificadas Tambieacuten es importante resaltar la necesidad de maacutes y mejores investigaciones
cliacutenicas
Para superar estas limitaciones el uso de ceacutelulas mesenquimales especificas (MSC) autoacutelogas
modificadas por BMP-2 ex vivo (Chung et al 2012) en los uacuteltimos antildeos estaacute dando lugar a
explorar nuevas estrategias como la encapsulacioacuten de la proteiacutena en diferentes biomateriales o el
suministro mediante terapia geacutenica
El desarrollo de estas tecnologiacuteas se basa en algunos hechos bioloacutegicos Los efectos in vitro de
las BMP se observan en dosis muy bajas (5-20 ngml) aunque las rhBMP actuales disponibles
comercialmente se usan en dosis grandes (hasta 40 mg de algunos productos) (A C Carreira et al
2014) Esto probablemente se deba a un consumo proteoliacutetico intenso durante las primeras fases
posquiruacutergicas Es importante conocer la secuencia adecuada de los procesos bioloacutegicos que
29
conducen a la cicatrizacioacuten normal del tejido Por lo tanto este conocimiento se puede usar para
intervenir en el marco temporal especiacutefico en el que se pretende que actuacutee nuestra terapia (Padial-
Molina et al 2012) Tambieacuten es importante tener en cuenta que el papel de otras viacuteas moleculares
y la diafoniacutea entre los diferentes componentes que llevan a cabo la regeneracioacuten oacutesea todaviacutea no
se entiende perfectamente y por lo tanto se debe realizar maacutes investigacioacuten
Lo que hasta ahora se sabe en resumen es que las BMP y especiacuteficamente BMP-2 son uacutetiles
para promover la regeneracioacuten oacutesea (A C Carreira et al 2014) Sin embargo las rutas disponibles
de administracioacuten local basadas en la activacioacuten de las BMP entregadas por esponjas de colaacutegeno
presentan importantes limitaciones (Chung et al 2012) En primer lugar la proteiacutena se inactiva
raacutepidamente Por lo tanto su accioacuten bioloacutegica desaparece puede ser incluso antes de que se forme
el coaacutegulo de sangre el cual se forma despueacutes de la cirugiacutea Ademaacutes la distribucioacuten de la BMP
en una suspensioacuten liacutequida incrustada en una esponja de colaacutegeno hace que sea imposible estar
seguro de que la proteiacutena estaacute alcanzando el objetivo ideal Debido a eso deben desarrollarse
nuevas formas de administracioacuten de BMP-2 Estas nuevas tecnologiacuteas tienen que garantizar una
mayor vida media de la proteiacutena y una liberacioacuten escalonada para aumentar los efectos sobre los
objetivos celulares deseados La biotecnologiacutea abre la puerta para poder proporcionar una
solucioacuten a estas limitaciones
De esta manera las nanopartiacuteculas biodegradables (nanoesferas y nanocaacutepsulas) fueron
desarrolladas como una herramienta importante y prometedora para la administracioacuten de
macromoleacuteculas a traveacutes de aplicaciones parenterales mucosas y toacutepicas (Barratt 2003)
(Bramwell and Perrie 2005) (Csaba Garcia-Fuentes and Alonso 2006) (M J Santander-Ortega
et al 2010) Los poliacutemeros biodegradables bien establecidos tales como poli (aacutecido D L-laacutectico)
o poli (D L-laacutectico-co-glicoacutelico) se estaacuten utilizando ampliamente en la preparacioacuten de
nanopartiacuteculas en las uacuteltimas deacutecadas debido a su biocompatibilidad y biodegradabilidad
completa (Jiang et al 2005) Sin embargo se sabe que ciertas macromoleacuteculas como proteiacutenas
o peacuteptidos pueden perder actividad durante su encapsulacioacuten almacenamiento administracioacuten y
30
liberacioacuten (Kumar Soppimath and Nachaegari 2006) Para superar este problema la adicioacuten de
estabilizadores tales como oacutexido de polietileno (PEO) o la co-encapsulacioacuten con otras
macromoleacuteculas y sus derivados parece ser una estrategia prometedora
12 Partiacuteculas coloidales polimeacutericas para encapsular moleacuteculas hidrofiacutelicas
Generalmente las partiacuteculas coloidales polimeacutericas son sistemas consistentes con una forma
esfeacuterica homogeacutenea compuesta por poliacutemeros naturales o sinteacuteticos Con el fin de encapsular
moleacuteculas hidroacutefilas como proteiacutenas o aacutecidos nucleicos para ello es necesario optimizar la
composicioacuten polimeacuterica y el meacutetodo de siacutentesis En este proceso se debe lograr una alta eficacia
de encapsulacioacuten el mantenimiento de la actividad bioloacutegica de la biomoleacutecula encapsulada y la
obtencioacuten de un patroacuten de liberacioacuten adecuado (Danhier et al 2012) (Kumari Yadav and Yadav
2010) (Makadia and Siegel 2011) Varios sistemas de administracioacuten de BMP2 (y otros GF) que
usan partiacuteculas polimeacutericas estaacuten descritos en la bibliografiacutea La mayoriacutea de ellos son sistemas
microparticulados Casi en su totalidad todos ellos usan el copoliacutemero de PLGA biocompatible
y biodegradable como componente principal (Mohamed and van der Walle 2008) (Silva et al
2007)
Teniendo en cuenta la incorporacioacuten de BMP2 al sistema portador la encapsulacioacuten es
preferible a la adsorcioacuten porque los factores de crecimiento estaacuten maacutes protegidos contra factores
ambientales en el medio y pueden tener un mejor control sobre la administracioacuten y liberacioacuten para
alcanzar las concentraciones deseadas en sitio y tiempo especiacuteficos (Zhang and Uludag 2009)
Normalmente si los GF estaacuten relacionados con los procesos de regeneracioacuten oacutesea las nano-
micropartiacuteculas quedan atrapadas en un segundo sistema como hidrogeles o scaffolds de
ingenieriacutea tisular que tambieacuten juegan un papel importante en el perfil de liberacioacuten de los GF de
estas partiacuteculas (Zhang and Uludag 2009) Las nano-micropartiacuteculas han permitido el desarrollo
de scaffolds multiescala lo que facilita el control de la arquitectura interna y los patrones
31
adecuados de los gradientes mecaacutenicos de las ceacutelulas asiacute como los factores de sentildealizacioacuten (Santo
et al 2012)
Todos los pasos desde el meacutetodo de siacutentesis y sus caracteriacutesticas el proceso de encapsulacioacuten
o la modificacioacuten final de la superficie para una entrega dirigida determinan las caracteriacutesticas de
estos sistemas y su objetivo principal la liberacioacuten controlada de GF bioactivos
Figura 2 Procedimiento de doble emulsioacuten (emulsioacuten agua aceite agua W1OW2) para
obtener micropartiacuteculas nanopartiacuteculas de PLGA
En la figura 2 se muestra el esquema de siacutentesis de micro-nanopartiacuteculas de PLGA mediante
un procedimiento de doble emulsioacuten Dependiendo de las condiciones de siacutentesis (estabilizadores
disolventes y procedimiento de mezcla) es posible obtener micro-nanoesferas con una matriz
uniforme o micro-nanocaacutepsulas con una estructura corteza-nuacutecleo Las inmunopartiacuteculas
32
utilizadas para la administracioacuten dirigida pueden obtenerse uniendo moleacuteculas especiacuteficas de
anticuerpos en la superficie de la partiacutecula
33
121Meacutetodos de siacutentesis
Es posible encontrar varios procedimientos para encapsular moleacuteculas hidrofiacutelicas como
proteiacutenas o aacutecidos nucleicos en nano micropartiacuteculas polimeacutericas Se ha observado que las
teacutecnicas de separacioacuten de fases (Tran Swed and Boury 2012) o secado por pulverizacioacuten (Ertl et
al 2000) encapsulan moleacuteculas hidroacutefilas Sin embargo en el caso de las proteiacutenas el
procedimiento maacutes utilizado para encapsularlas en micropartiacuteculas y nanopartiacuteculas de PLGA es
la teacutecnica de evaporacioacuten con disolvente de doble emulsioacuten (WOW) (Makadia and Siegel 2011)
(Hans and Lowman 2002) En la figura 2 se presenta una descripcioacuten esquemaacutetica de esta teacutecnica
De manera general el PLGA se disuelve en un disolvente orgaacutenico y se emulsiona usando
agitacioacuten mecaacutenica o sonicacioacuten con agua que contiene una cantidad apropiada de proteiacutena
Por lo tanto se obtiene una emulsioacuten W1O primaria En la segunda fase esta emulsioacuten se
vierte en una gran fase polar que conduce a una precipitacioacuten inmediata de las partiacuteculas como
consecuencia de la contraccioacuten del poliacutemero alrededor de las gotitas de la emulsioacuten primaria Esta
fase puede estar compuesta por una solucioacuten acuosa de un estabilizador (surfactante) o mezclas
de etanol y agua (Blanco and Alonso 1998) (Csaba et al 2005) Despueacutes de agitar el resultado
del disolvente orgaacutenico se extrae raacutepidamente por evaporacioacuten al vaciacuteo En este procedimiento se
ha probado una amplia lista de diferentes modificaciones con el fin de obtener un sistema micro
nanoportador con estabilidad coloidal adecuada alta eficacia de encapsulacioacuten bioactividad
adecuada y finalmente un perfil de liberacioacuten a largo plazo con una miacutenima descarga inicial
El objetivo es evitar que se libere una gran cantidad de proteiacutena (gt 60) muy raacutepidamente (24
horas) que es uno de los mayores problemas de un sistema de liberacioacuten controlada (Mohamed
and van der Walle 2008)
34
122 Disolvente orgaacutenico
Hans y colaboradores muestran diferentes ejemplos de solventes orgaacutenicos usados en muacuteltiples
procesos de emulsioacuten Normalmente pueden usarse diclorometano (DMC) acetato de etilo
acetona y otras mezclas (Hans and Lowman 2002) En el primer paso seriacutea una buena eleccioacuten
escoger un buen solvente orgaacutenico con baja solubilidad en agua para facilitar el proceso de
emulsioacuten y bajo punto de ebullicioacuten para una faacutecil evaporacioacuten Sin embargo la estructura de las
moleacuteculas de proteiacutenas encapsuladas puede verse afectada y los procesos de desnaturalizacioacuten y
peacuterdida de actividad bioloacutegica aparecen cuando interactuacutean con un solvente orgaacutenico tiacutepico como
DMC (Danhier et al 2012) El acetato de etilo por otro lado ejerce efectos menos
desnaturalizantes con una menor incidencia en la bioactividad de las proteiacutenas encapsuladas
(Sturesson and Carlfors 2000)
Otros factores importantes relacionados con el disolvente orgaacutenico son sus propiedades fiacutesicas
que afectan la forma en que las moleacuteculas del poliacutemero se auto organizan en la envoltura de las
gotas de la emulsioacuten y modifican la morfologiacutea de las nanopartiacuteculas y la eficacia de
encapsulacioacuten (Rosca Watari and Uo 2004) De esta forma una mayor solubilidad en agua del
disolvente orgaacutenico es decir acetato de etilo favorece una eliminacioacuten maacutes raacutepida del disolvente
Ademaacutes la velocidad de eliminacioacuten del disolvente puede controlarse ajustando el volumen de la
fase polar asiacute como la tensioacuten de cizallamiento durante la segunda etapa de la emulsioacuten Un
aumento de estos dos paraacutemetros aumenta la velocidad de difusioacuten del acetato de etilo desde las
micropartiacuteculas primarias a la fase acuosa externa lo que da como resultado su raacutepida
solidificacioacuten (Meng et al 2003) Tambieacuten mejora la eficiencia de encapsulacioacuten y minimiza el
tiempo de contacto entre las moleacuteculas de proteiacutena y el solvente orgaacutenico (Ghaderi and Carlfors
1997) obteniendo al mismo tiempo un menor efecto de raacutefaga y una liberacioacuten maacutes lenta del
faacutermaco desde las micropartiacuteculas (Meng et al 2003)
35
123 Tamantildeo y morfologiacutea de las partiacuteculas
El tamantildeo de la partiacutecula es un paraacutemetro importante y uno de los objetivos principales del
sistema de liberacioacuten polimeacuterica Las microesferas desde unos pocos microacutemetros hasta 100 μm
son adecuadas para el suministro oral la adhesioacuten a la mucosa o el uso interior del armazoacuten es
decir para la regeneracioacuten oacutesea La dimensioacuten a nano-escala del soporte ofrece una versatilidad
mejorada cuando se compara con partiacuteculas de mayor tamantildeo Esto se debe a que tienen una
mayor estabilidad coloidal una mejor dispersabilidad y biodisponibilidad una superficie maacutes
reactiva y ademaacutes pueden administrar proteiacutenas o faacutermacos dentro y fuera de las ceacutelulas
correspondientes (Wang et al 2012) BMP2 promueve la formacioacuten de hueso e induce la
expresioacuten de otras BMP e inicia la viacutea de sentildealizacioacuten desde la superficie de la ceacutelula unieacutendose
a dos receptores de superficie diferentes (Bustos-Valenzuela et al 2011) Por lo tanto las
partiacuteculas portadoras de BMP2 deben liberarlo en el medio extracelular Dado que la ingesta
celular de nanopartiacuteculas de PLGA es muy raacutepida el proceso de incorporacioacuten puede verse
limitado por un aumento en el tamantildeo de nano a micropartiacuteculas (Xiong et al 2011) Sin
embargo la interaccioacuten entre partiacuteculas y ceacutelulas estaacute fuertemente influenciada por el tamantildeo de
la partiacutecula Si se desea la internalizacioacuten de la ceacutelula la partiacutecula debe estar comprendida en la
escala submicroacutemica en un intervalo entre 2-500 nm (Chou Ming and Chan 2011) Ademaacutes este
tamantildeo es necesario para una distribucioacuten raacutepida despueacutes de la administracioacuten parenteral con el
fin de alcanzar diferentes tejidos a traveacutes de diferentes barreras bioloacutegicas Ademaacutes la ingesta de
macroacutefagos se minimiza con un diaacutemetro de nanopartiacuteculas por debajo de 200 nm e incluso maacutes
pequentildeo (Hans and Lowman 2002) (Manuel J Santander-Ortega Lozano-Loacutepez et al 2010)
Como se discutioacute en un artiacuteculo escrito por Yang y colaboradores (Yang Chung and Ng 2001)
ligeras modificaciones del procedimiento de siacutentesis pueden suponer efectos draacutesticos en el
tamantildeo o la morfologiacutea de las partiacuteculas y por lo tanto en la eficacia de encapsulacioacuten de
proteiacutenas y la liberacioacuten cineacutetica
36
Figura 3 Fotografiacutea mediante microscopiacutea electroacutenica de barrido (SEM) de nanopartiacuteculas
de PLGA obtenidas mediante un procedimiento de emulsificacioacuten de doble emulsioacuten Es un
sistema con forma esfeacuterica baja polidispersidad y una escala nanoscoacutepica que muestra las
propiedades deseadas para una distribucioacuten fisioloacutegica adecuada y la internalizacioacuten celular
En los procesos de doble emulsioacuten la primera etapa de emulsioacuten determina en gran medida el
tamantildeo de la partiacutecula mientras que la segunda etapa de emulsioacuten caracterizada por la
eliminacioacuten del disolvente y la precipitacioacuten del poliacutemero afecta principalmente a la morfologiacutea
de la partiacutecula (Rosca Watari and Uo 2004) Sin embargo en este paso el uso de soluciones
surfactantes como el medio polar del segundo proceso de emulsioacuten y la relacioacuten de volumen entre
las fases orgaacutenicas y polares han mostrado una influencia importante en el tamantildeo final (Feczkoacute
Toacuteth and Gyenis 2008)Por lo tanto la eleccioacuten correcta del solvente orgaacutenico la concentracioacuten
del poliacutemero la adicioacuten de surfactante y la energiacutea del proceso de emulsioacuten permiten controlar el
tamantildeo del sistema
37
La incorporacioacuten de poloxaacutemeros (F68) en el disolvente orgaacutenico de la emulsioacuten primaria
ayuda a aumentar la estabilidad coloidal de la primera dispersioacuten al colocarla en el interfaz WO
Esto reduce el tamantildeo de partiacutecula en comparacioacuten con las nanopartiacuteculas de PLGA puro en las
que la uacutenica fuente de estabilidad proviene de la carga eleacutectrica de los grupos carboxilo del PLGA
(Santander-Ortega Bastos-Gonzalez and Ortega-Vinuesa 2007) Es normal obtener micro
nanoesferas ciliacutendricas con un nuacutecleo poroso polimeacuterico En la figura 3 se muestra una
micrografiacutea SEM tiacutepica de nanopartiacuteculas de PLGA obtenidas mediante emulsioacuten WOW usando
una mezcla de disolventes orgaacutenicos (DCM acetona) y etanol agua como segundo medio polar
en la que la forma esfeacuterica y la distribucioacuten uniforme del tamantildeo son las principales
caracteriacutesticas La cubierta exterior polimeacuterica en la segunda etapa de emulsioacuten empujoacute las gotas
de agua hacia el nuacutecleo interno de acuerdo con el proceso de solidificacioacuten (Yang Chia and
Chung 2000) Este proceso permite producir partiacutecula como son estas caacutepsulas con una
estructura nuacutecleo-capa en la que el nuacutecleo interno tiene una baja densidad de poliacutemero
La figura 4 muestra una estructura nuacutecleo-capa tiacutepica en la que el poliacutemero precipita y se
contrae alrededor de las gotas de agua durante el cambio de disolvente de la segunda fase y el
posterior proceso de evaporacioacuten del disolvente orgaacutenico (Fang et al 2014) En este caso el
proceso de solidificacioacuten del poliacutemero se ve influenciado y determinado por la miscibilidad del
disolvente orgaacutenico con la segunda fase polar y la velocidad de eliminacioacuten
38
Figura 4 Nanopartiacuteculas de mezcla PLGA poloxamers188 (a) Fotografiacutea de microscopiacutea
electroacutenica de transmisioacuten (STEM) (b) Fotografiacutea de microscopiacutea electroacutenica de barrido (SEM)
La teacutecnica STEM permite el anaacutelisis de la estructura de nanopartiacuteculas con una regioacuten interna
con baja densidad de poliacutemero que es representativa de nanocaacutepsulas con estructura nuacutecleo-
caparazoacuten
La cubierta polimeacuterica a menudo presenta canales o poros como consecuencia de la extrusioacuten
de agua interna debido a las fuerzas osmoacuteticas Esto puede reducir la eficacia de la encapsulacioacuten
y favorecer una raacutepida fuga inicial con la liberacioacuten en raacutefaga no deseada (Yang Chung and
Ng 2001) Esta modificacioacuten de la estructura interna de las partiacuteculas generalmente se indica
asignando el teacutermino nanoesfera al sistema con un nuacutecleo que consiste en una matriz polimeacuterica
homogeacutenea El agente bioactivo se dispersa dentro de ellas mientras que la estructura nuacutecleo-
capa seriacutea similar a una nanocaacutepsula donde la biomoleacutecula estaacute preferiblemente en la cavidad
acuosa rodeada por la cubierta polimeacuterica (Zhang and Uludag 2009) (ver figura 2)
39
13Agentes estabilizadores
131 Estabilidad coloidal
El meacutetodo de doble emulsioacuten normalmente requiere la presencia de estabilizadores para
conferir estabilidad coloidal durante la primera etapa de emulsioacuten para evitar la coalescencia de
las gotas de la emulsioacuten y maacutes tarde para mantener la estabilidad de las nano micropartiacuteculas
finales (Ratzinger et al 2010) El alcohol poliviniacutelico (PVA) y el derivado de PEO como
poloxaacutemeros se han usado en la mayoriacutea de los casos (Blanco and Alonso 1998) (Feczkoacute Toacuteth
and Gyenis 2008) Otros incluyen surfactantes naturales como los fosfoliacutepidos (Feng and Huang
2001) (Chan et al 2009) En algunos casos es posible evitar los surfactantes si las partiacuteculas
tienen una contribucioacuten de estabilidad electrostaacutetica es decir de los grupos carboxilo terminales
no protegidos de las moleacuteculas de PLGA (Fraylich et al 2008)
Como se ha comentado anteriormente el PVA y los poloxaacutemeros han demostrado su eficacia
en la siacutentesis de nanopartiacuteculas y micropartiacuteculas afectando no solo la estabilidad de los sistemas
sino tambieacuten su tamantildeo y morfologiacutea Por lo tanto se ha encontrado un efecto de reduccioacuten de
tamantildeo usando PVA en la fase acuosa externa que afecta al mismo tiempo la porosidad
superficial principalmente en partiacuteculas de tamantildeo micro (Feczkoacute Toacuteth and Gyenis 2008) Un
estudio comparativo entre PVA y fosfoliacutepidos (di-palmitoil fosfatidilcolina) como estabilizadores
mostroacute que DPPC podriacutea ser un mejor emulsionante que PVA para producir nano y
micropartiacuteculas Con este meacutetodo se necesitaba una cantidad de estabilizador mucho maacutes baja
para obtener un tamantildeo similar En el mismo estudio se demostroacute una mayor porosidad en la
superficie de la partiacutecula para las nanoesferas emulsionadas con PVA (Feng and Huang 2001)
Por otro lado la combinacioacuten de PLGA con poloxaacutemeros ha mostrado efectos positivos para
los nano y microsistemas en teacuterminos de estabilidad eficacia de encapsulacioacuten o caracteriacutesticas
de liberacioacuten controlada (Santander-Ortega et al 2011) El uso de estos surfactantes en el primer
o segundo paso del procedimiento de emulsioacuten WOW conduce a situaciones diferentes Por lo
40
tanto si los poloxaacutemeros se mezclan con PLGA en la fase orgaacutenica de la emulsioacuten primaria se
obtiene una alteracioacuten de la rugosidad superficial Sin embargo si se agregan en la fase de agua
interna se encuentra un aumento de la porosidad (Blanco and Alonso 1998) La inclusioacuten de
poloxaacutemeros en la fase polar de la segunda etapa de emulsioacuten tambieacuten genera superficies de
rugosidad hidroacutefila Una cuantificacioacuten de esto se muestra en la figura 5 en la que se mide la
movilidad electroforeacutetica de PLGA puro y PLGA pluronic F68 nanopartiacuteculas como una funcioacuten
del pH del medio La composicioacuten de superficie diferente afecta el comportamiento
electrocineacutetico de las nanopartiacuteculas desnudas La carga superficial se modula por la presencia de
tensioactivo no ioacutenico como poloxaacutemeros o en mayor medida por la presencia de moleacuteculas de
anticuerpos unidos en la superficie La dependencia observada con este paraacutemetro es una
consecuencia del caraacutecter aacutecido deacutebil de los grupos carboxilo de PLGA Cuando las moleacuteculas de
poloxaacutemero estaacuten presentes en la interfaz se encuentra una reduccioacuten sistemaacutetica de la movilidad
como consecuencia del aumento de la rugosidad superficial Las cadenas de surfactante hidroacutefilo
se dispersan hacia el disolvente originando un desplazamiento del plano de corte y la consecuente
reduccioacuten de movilidad (Santander-Ortega Bastos-Gonzalez and Ortega-Vinuesa 2007)
(Fraylich et al 2008) La presencia de moleacuteculas proteicas en la superficie introduce una
dependencia de la carga neta superficial con el punto isoeleacutectrico de eacutestas situacioacuten que se refleja
en el comportamiento electrocineacutetico que incluso muestras valores positivos en pHs inferiores al
punto isoeleacutectrico del anticuerpo
El tamantildeo final de las partiacuteculas de PLGA se controla principalmente por fuerzas
electrostaacuteticas y no se ve significativamente afectado por la presencia o la naturaleza de los
estabilizadores de poloxaacutemero (Fraylich et al 2008) El reconocimiento de los nanovehiacuteculos por
el sistema fagociacutetico mononuclear (MPS) se puede alterar significativamente si la superficie de
las partiacuteculas coloidales se modifica mediante el uso de copoliacutemeros de bloque PEO de las
moleacuteculas de poloxaacutemero La barrera esteacuterica proporcionada por estas moleacuteculas surfactantes
previenen o minimizan la adsorcioacuten de proteiacutenas plasmaacuteticas y disminuye el reconocimiento por
41
los macroacutefagos (Tan et al 1993) El tamantildeo de las microesferas tampoco se ve afectado por la
encapsulacioacuten de poloxaacutemeros El sistema que contiene mezclas de poloxaacutemero-PLGA conduce
a una estructura interna que muestra pequentildeos orificios y cavidades en relacioacuten con microesferas
de PLGA puro con una estructura de tipo matriz compacta (Blanco and Alonso 1998)
Figura 5 Movilidad electroforeacutetica versus pH para nanopartiacuteculas de PLGA con diferentes
caracteriacutesticas () PLGA (◼) mezcla de PLGA poloxamer188 y () PLGA cubierto por
Immuno-γ-globulina
Las micropartiacuteculas formuladas por poloxaacutemero en el segundo medio polar tienen una
superficie completamente diferente que las de PVA casi sin poros (Feczkoacute Toacuteth and Gyenis
2008) Una comparacioacuten entre diferentes poloxaacutemeros muestra que el balance hidroacutefilo-lipoacutefilo
(HBL) del surfactante juega un papel crucial determinando las interacciones surfactante-poliacutemero
y controlando la porosidad y la rugosidad de las nano-micropartiacuteculas (Blanco and Alonso 1998)
(Bouissou et al 2004)
42
De manera similar a los surfactantes las caracteriacutesticas del poliacutemero como el grado de
hidrofobicidad el peso molecular o la velocidad de degradacioacuten de la hidroacutelisis pueden influir
fuertemente en la morfologiacutea de la partiacutecula Por lo tanto la composicioacuten polimeacuterica de las
partiacuteculas afecta en gran medida su estructura y propiedades Es por eso que es habitual usar otros
poliacutemeros para modificar el comportamiento y la aplicacioacuten de las partiacuteculas De esta manera el
polietilenglicol (PEG) de diferente longitud de cadena se usa frecuentemente para modificar las
caracteriacutesticas de la superficie Con PEG las partiacuteculas son maacutes hidroacutefilas y tienen superficies
maacutes rugosas que afectan la accioacuten de MPS al aumentar el tiempo de circulacioacuten y la vida media
in vivo como la presencia de cadenas de PEO (Gref et al 1994) Ademaacutes las cadenas de PEG
tambieacuten proporcionan estabilidad coloidal a traveacutes de la estabilizacioacuten esteacuterica Las
nanopartiacuteculas o micropartiacuteculas de PLGA se pueden obtener normalmente mediante el uso en el
meacutetodo de siacutentesis de copoliacutemeros de di y tri-bloque de PLGA PEG (Lochmann et al 2010)
(White et al 2013) (Makadia and Siegel 2011) Los poliacutemeros naturales como el quitosano
ademaacutes de aumentar la hidrofobicidad de la superficie tambieacuten les confieren un caraacutecter
mucoadherente (Paolicelli et al 2010)
132 Eficacia de encapsulacioacuten y bioactividad
Ademaacutes el uso de estabilizantes (surfactantes o poliacutemeros) tambieacuten influye en la eficacia de
encapsulacioacuten y la estabilidad de la proteiacutena De hecho para el proceso de evaporacioacuten del
solvente WOW el solvente orgaacutenico clorado usado para la primera emulsioacuten puede degradar las
moleacuteculas de proteiacutena encapsuladas en este paso si entran en contacto con la interfaz orgaacutenica
agua causando su agregacioacuten o desnaturalizacioacuten (Brigger Dubernet and Couvreur 2002) La
interaccioacuten poliacutemero-proteiacutena el estreacutes de cizallamiento para el proceso de emulsioacuten y la
reduccioacuten del pH derivada de la degradacioacuten del poliacutemero PLGA tambieacuten pueden producir la
misma situacioacuten con la peacuterdida posterior de la actividad bioloacutegica de las biomoleacuteculas
encapsuladas Se han usado diferentes estrategias para prevenirlo Por ejemplo un aumento de la
viscosidad alrededor de las moleacuteculas de proteiacutenas puede ayudar a aislarlas de su microambiente
43
(Giteau et al 2008) De esta forma los productos viscosos como el almidoacuten se han utilizado
para prevenir la inestabilidad proteica (Balmayor et al 2009) Estos autores coencapsulan BMP2
con albuacutemina dentro de micropartiacuteculas de almidoacuten usando otro poliacutemero biodegradable poli-ε-
caprolactona en lugar de PLGA La BMP2 retuvo la bioactividad A pesar de una baja tasa de
encapsulacioacuten ademaacutes de una raacutefaga inicial seguida de una liberacioacuten incompleta la cantidad de
BMP2 necesaria al principio fue menor (Balmayor et al 2009) La combinacioacuten de surfactantes
PEO con PLGA (mezclados en la fase orgaacutenica) tambieacuten puede preservar la bioactividad de las
proteiacutenas microencapsuladas (Santander-Ortega et al 2009) o los aacutecidos nucleicos (Csaba et al
2005)
Sin embargo en la mayoriacutea de los casos la estrategia preferida fue la coencapsulacioacuten de
estabilizadores con biomoleacuteculas De este modo las albuacuteminas seacutericas (SA) han demostrado la
capacidad de limitar la agregacioacuten-desestabilizacioacuten de varias proteiacutenas incitadas por el interfaz
agua disolvente orgaacutenico del proceso de emulsioacuten primaria (Meinel et al 2001) (Srinivasan et
al 2005) White y colaboradores en uno de sus estudios encapsularon lisozima dentro de
micropartiacuteculas de PLGA-PEG Ademaacutes de la funcioacuten de proteccioacuten tambieacuten observaron un
aumento importante de la eficiencia de encapsulacioacuten cuando el SA humana se co-encapsuloacute con
lisozima y BMP2 (White et al 2013) Dr Angelo y colaboradores usaron heparina como
estabilizador porque eacutesta forma un complejo especiacutefico con varios factores de crecimiento
estabiliza su estructura tridimensional y promueve su bioactividad Se consiguioacute aumentar asiacute la
eficacia de encapsulacioacuten del 35 al 87 usando SA bovina como un segundo estabilizador para
encapsular dos factores de crecimiento proangiogeacutenico naturales dentro de las nanopartiacuteculas
mezcladas con PLGA-poloxaacutemero Los ensayos celulares in vitro mostraron la preservacioacuten de la
actividad bioloacutegica de GF hasta un mes despueacutes (drsquoAngelo et al 2010)
El uso de maacutes surfactantes hidroacutefilos (poloxaacutemeros) o poliacutemeros (PEG) en la fase acuosa
interna o durante la mezcla del PLGA en la fase orgaacutenica de la emulsioacuten primaria reduce la
interaccioacuten de las proteiacutenas encapsuladas con la matriz de PLGA hidroacutefoba Esto evita la
44
alteracioacuten de la estructura de las moleacuteculas de proteiacutena y ayuda al mismo tiempo a neutralizar la
acidez generada por la degradacioacuten hidroliacutetica del PLGA (Tan et al 1993) En algunos casos se
ha demostrado que la combinacioacuten de varios estabilizadores como poloxaacutemeros tranexaacutemico y
bicarbonato de sodio preserva la integridad de las proteiacutenas encapsuladas pero tambieacuten reduce
la eficacia de la encapsulacioacuten (Bouissou et al 2004)
Como regla general la eficacia de la encapsulacioacuten aumenta con el tamantildeo de las partiacuteculas
(Hans and Lowman 2002) Ademaacutes la estabilizacioacuten adecuada de la emulsioacuten primaria por
poliacutemeros anfifiacutelicos y una solidificacioacuten (precipitacioacuten) raacutepida del poliacutemero en el segundo paso
son paraacutemetros favorables para mejorar la eficacia de encapsulacioacuten de proteiacutenas en la teacutecnica de
emulsioacuten WOW (Meng et al 2003)
La tendencia de BMP2 a interactuar con superficies hidrofoacutebicas puede disminuir la peacuterdida
de proteiacutena encapsulada durante la liberacioacuten de la fase de disolvente Esto favorece una mayor
encapsulacioacuten pero disminuye la liberacioacuten posterior (Lochmann et al 2010) Se obtiene una
encapsulacioacuten proteica oacuteptima cuando el pH de las fases de agua internas y externas estaacuten cerca
del punto isoeleacutectrico de la proteiacutena (Manuel J Santander-Ortega Lozano-Loacutepez et al 2010)
Blanco y Alonso (Blanco and Alonso 1998) observaron una reduccioacuten en la eficacia de
encapsulacioacuten de proteiacutena cuando el poloxaacutemero se coencapsuloacute en la emulsioacuten primaria Esto
pone de relieve el papel principal desempentildeado por la interaccioacuten proteiacutena-poliacutemero en la eficacia
de encapsulacioacuten y el proceso de liberacioacuten posterior Sin embargo demasiado emulsionante
tambieacuten puede dar como resultado una reduccioacuten de la eficacia de encapsulacioacuten (Feng and
Huang 2001) Por lo tanto se necesita un equilibrio entre el polvo de emulsioacuten del surfactante y
su concentracioacuten
14 Patroacuten de liberacioacuten
El patroacuten de liberacioacuten representa una de las caracteriacutesticas maacutes importantes de un sistema
portador de partiacuteculas nano micro ya que su desarrollo tiene un objetivo final principal la
45
liberacioacuten adecuada de las moleacuteculas bioactivas encapsuladas para alcanzar la accioacuten cliacutenica
deseada
Se han definido diferentes patrones de liberacioacuten de proteiacutenas encapsuladas en micropartiacuteculas
nanopartiacuteculas de PLGA Es posible encontrar una liberacioacuten continua cuando la difusioacuten de la
biomoleacutecula es maacutes raacutepida que la erosioacuten de la partiacutecula Este proceso implica una difusioacuten
continua de la proteiacutena que se encuentra en la matriz del poliacutemero antes de que la partiacutecula de
PLGA se degrade en monoacutemeros de aacutecido laacutectico y glicoacutelico por hidroacutelisis (Kumari Yadav and
Yadav 2010) Tambieacuten se ha descrito una liberacioacuten bifaacutesica caracterizada por una descarga
inicial dentro o cerca de la superficie de la partiacutecula seguido por una segunda fase en la que la
proteiacutena se libera progresivamente por difusioacuten La segunda fase se puede mejorar mediante la
erosioacuten masiva del caparazoacuten y la matriz de PLGA lo que da como resultado un importante
aumento de poros y canales (Makadia and Siegel 2011) Se ha encontrado un tercer perfil de
liberacioacuten trifaacutesica cuando se produce un periacuteodo de liberacioacuten de retardo despueacutes de la descarga
inicial y hasta la degradacioacuten del poliacutemero (Cleland 1997) Finalmente es posible obtener una
liberacioacuten de proteiacutena incompleta como consecuencia de factores adicionales relacionados con la
interaccioacuten proteiacutena-poliacutemero o la inestabilidad proteica La Figura 6 ilustra los diferentes perfiles
de liberacioacuten descritos anteriormente Un sistema de transporte oacuteptimo deberiacutea ser capaz de liberar
un gradiente de concentracioacuten controlado de factores de crecimiento en el momento apropiado
evitando o al menos reduciendo o controlando el efecto de descarga inicial (Oh Kim and Lee
2011) Una explosioacuten inicial controlada seguida de una liberacioacuten sostenida mejora
significativamente la regeneracioacuten oacutesea in vivo (Brown et al 2009) (Brown et al 2011) (Li et
al 2009)
Giteau y colaboradores (Giteau et al 2008) presentan una revisioacuten interesante sobre Coacutemo
lograr una liberacioacuten sostenida y completa de micropartiacuteculas de PLGA Comienzan por analizar
la influencia del medio de liberacioacuten y el meacutetodo de muestreo en el perfil de liberacioacuten y resaltan
la importancia del proceso de limpieza por centrifugacioacuten o el volumen del medio de liberacioacuten
46
Ajustando a valores adecuados la velocidad de centrifugacioacuten o el volumen del tampoacuten es posible
separar micro nanopartiacuteculas del medio de liberacioacuten que contiene proteiacutenas de una manera muy
faacutecil Esto permite patrones de liberacioacuten estables y reproducibles Por otro lado para garantizar
un mejor perfil de liberacioacuten de proteiacutenas se debe realizar la modificacioacuten de la formulacioacuten de
micropartiacuteculas y el proceso de microencapsulacioacuten para preservar la agregacioacuten de proteiacutenas La
estabilidad de la proteiacutena debe mantenerse evitando la formacioacuten de un medio dantildeino Por
ejemplo la formulacioacuten de una siacutentesis en concreto puede modificarse para usar poliacutemeros maacutes
hidroacutefilos ya que se ha demostrado que reducen el estallido inicial y proporcionan proteiacutenas
bioactivas durante largos periodos de tiempo
47
Figura 6 Patroacuten de liberacioacuten (liacutenea negra) Cineacutetica de liberacioacuten de BSA en nanopartiacuteculas
de PLGA con alta liberacioacuten inicial (liacutenea de puntos rojos) modelo bifaacutesico que combina un
estallido inicial moderado y una liberacioacuten sostenida posterior (liacutenea de trazos azules) modelo
trifaacutesico con un retraso de liberacioacuten entre las fases de liberacioacuten inicial y sostenida (liacutenea verde
de guiones) liberacioacuten incompleta
Las estrategias maacutes relevantes se mencionan a continuacioacuten La liberacioacuten de faacutermaco a partir
de nano micropartiacuteculas de PLGA puede controlarse por el peso molecular del poliacutemero y la
relacioacuten entre monoacutemeros (laacutectico glicoacutelico) de forma que un aumento del aacutecido glicoacutelico
acelera la peacuterdida de peso del poliacutemero debido a la mayor hidrofilicidad de la matriz (Makadia
and Siegel 2011)
Por otro lado se ha descrito una erosioacuten maacutes raacutepida de las microesferas con reduccioacuten en el
peso molecular de PLGA debido a la facilidad de penetracioacuten del agua y la posterior degradacioacuten
del poliacutemero (Blanco and Alonso 1998) Schrier y colaboradores trabajaron con microesferas
48
preparadas por WOW utilizando diferentes tipos de PLGA analizaron el importante papel del
peso molecular la relacioacuten laacutectico-glicoacutelico y los residuos de aacutecido (Schrier et al 2001) La
cantidad de rhBMP2 adsorbido en la superficie de la micropartiacutecula aumentoacute con la
hidrofobicidad del poliacutemero Al mismo tiempo la liberacioacuten estaba en correlacioacuten con el perfil
de degradacioacuten de los diferentes poliacutemeros (Schrier et al 2001)
Por lo tanto el uso de poliacutemeros maacutes hidroacutefilos reduce la interaccioacuten proteiacutena hidroacutefoba-
poliacutemero Este efecto favorece una distribucioacuten maacutes homogeacutenea en la matriz polimeacuterica y
aumenta la absorcioacuten de agua en las microesferas Por lo que la velocidad de liberacioacuten de
rhBMP2 encapsulada en microesferas compuestas por un copoliacutemero de di-bloque PEG-PLGA
se incrementa con el contenido de PEG de la matriz de poliacutemero (Lochmann et al 2010) Se
obtuvo un resultado similar utilizando copoliacutemeros tri-bloque PLGA-PEG-PLGA (White et al
2013) En este caso modificando la relacioacuten del monoacutemero (laacutectido-glicoacutelido) en el PLGA y
aumentando la cantidad de PLGA-PEG-PLGA en las formulaciones el patroacuten de liberacioacuten de
BMP-2 co-encapsulada con HSA en microesferas fue ajustable
Por otro lado el uso de mezclas de PLGA-poloxaacutemeros es uacutetil para obtener una liberacioacuten
sostenida durante maacutes de un mes sin incidencia de producirse una descarga inicial (drsquoAngelo et
al 2010) (Manuel J Santander-Ortega Lozano-Loacutepez et al 2010) Sin embargo para un
plaacutesmido encapsulado dentro de nanopartiacuteculas obtenidas mediante mezclas de PLGA-
poloxaacutemero la hidrofobicidad del surfactante permite prolongar la liberacioacuten hasta 2 semanas de
una manera controlada Por otra parte se alcanzoacute una liberacioacuten completa para la mezcla de
PLGA-poloxaacutemero en lugar de nanopartiacuteculas de PLGA en la que la liberacioacuten maacutexima fue de
alrededor del 40 (Csaba et al 2005)
Las mezclas de PLGA y poloxaacutemeros (pluronic F68) tambieacuten se pueden usar para obtener
vesiacuteculas o caacutemaras nanocompuestas mediante un proceso de doble emulsioacuten Estas vesiacuteculas son
adecuadas para la encapsulacioacuten de moleacuteculas hidrofoacutebicas e hidrofiacutelicas La presencia de
pluronic afecta la estabilidad coloidal de las vesiacuteculas y el patroacuten de liberacioacuten de las moleacuteculas
49
encapsuladas Estas vesiacuteculas presentan una pared de 30 nm y el faacutermaco estaacute encapsulado en
presencia del poloxaacutemero (Nair and Sharma 2012)
Otras estrategias incluyen el uso de diferentes compuestos para aumentar el tiempo de
liberacioacuten Por lo tanto BMP2 encapsulada en nanopartiacuteculas de PLGA-PVA (alrededor de 300
nm) mostroacute una mayor eficiencia de encapsulacioacuten y un perfil de liberacioacuten de corta duracioacuten con
un estallido inicial muy alto De forma similar las nanopartiacuteculas de mezcla PLGA-poloxaacutemero
se modificaron superficialmente introduciendo quitosano en el segundo paso de la siacutentesis Este
meacutetodo mostroacute un perfil de liberacioacuten sostenida de hasta 14 diacuteas sin ninguacuten estallido importante
inicial En este caso se utilizoacute un antiacutegeno recombinante de hepatitis B (Paolicelli et al 2010)
Ademaacutes el uso de heparina conjugada con microesferas porosas de PLGA tambieacuten se ha descrito
para obtener un sistema de administracioacuten a largo plazo que reduce al mismo tiempo el estallido
inicial En estos sistemas la heparina se inmovilizoacute en la superficie nano micropartiacutecula La
liberacioacuten se controloacute usando las afinidades de unioacuten de la heparina a varios factores de
crecimiento incluida la BMP2 En este caso el estallido inicial se redujo hasta un 4-7 durante
el primer diacutea seguido de una liberacioacuten sostenida de aproximadamente un 1 por diacutea (La et al
2010) (Chung et al 2007) (Jeon et al 2008)
La liberacioacuten de proteiacutena en la descarga inicial puede atenuarse mediante la fabricacioacuten de
microesferas de doble pared es decir micropartiacuteculas con nuacutecleo y concha La presencia de un
revestimiento o armazoacuten de PLA reduce la tasa de liberacioacuten de BSA encapsulado en el nuacutecleo
PLGA y extiende la duracioacuten del perfil de liberacioacuten hasta dos meses Por otra parte un aumento
en el peso molecular de PLA influye en la tasa de erosioacuten de las partiacuteculas lo que ralentiza auacuten
maacutes la liberacioacuten de proteiacutenas (Xia et al 2013)
La modificacioacuten de la viscosidad en el entorno de micropartiacuteculas influye adicionalmente en
el patroacuten de liberacioacuten La viscosidad puede controlar el estallido en el punto maacutes temprano y
promover una liberacioacuten sostenida Esta situacioacuten se ha demostrado para las microesferas de
rhBMP2-PLGA incrustadas en un hidrogel de aacutecido quitosano-tioglicoacutelico (Poloxaacutemero 407) (Fu
50
et al 2012) Yilgor y colaboradores tambieacuten incorporaron las nanopartiacuteculas de su sistema de
administracioacuten secuencial a un scaffolds compuesto por quitosano y quitosano-PEO (Yilgor et al
2009) En otro trabajo las microesferas de PLGA PVA con BMP2 encapsulado se combinaron
con diferentes biomateriales compuestos (hidrogel de gelatina o fumarato de polipropileno) La
liberacioacuten sostenida de la moleacutecula bioactiva se extendioacute durante un periacuteodo de 42 diacuteas Los
resultados in vivo indican la importancia de las caracteriacutesticas compuestas En este caso se obtuvo
una mejor formacioacuten de hueso cuando las micropartiacuteculas de PLGA se incorporaron a la matriz
maacutes hidroacutefoba (fumarato de polipropileno) (Kempen et al 2008) (Kempen et al 2009)
Finalmente la tabla 1 resume informacioacuten importante sobre diferentes paraacutemetros relacionados
con el uso de nano o micropartiacuteculas basadas en PLGA para encapsular transportar y liberar
factores de crecimiento (principalmente BMP2) La mayoriacutea de ellos estaacuten en la escala
microscoacutepica El PVA ha sido el estabilizador de surfactante maacutes utilizado Es posible encontrar
tanto la encapsulacioacuten como la adsorcioacuten de superficie de los factores de crecimiento con una
eficiencia alta a moderada El uso de heparina como estabilizador reduce significativamente la
liberacioacuten inicial en estallido favoreciendo una liberacioacuten sostenida en el tiempo La bioactividad
del GF se conservoacute en la mayoriacutea de los sistemas y la encapsulacioacuten con otras biomoleacuteculas parece
tener un efecto similar al del uso de surfactantes como estabilizadores
51
TABLA 1 Sistemas de nano micropartiacuteculas para encapsular GF principalmente el factor
de crecimiento BMP2
Poliacutemeros Estabilizadores Tamantildeo EE
Encapsulacioacuten Liberacioacuten
Actividad
bioloacutegica Referencia
PLGA PVA 10-20 microm Adsorcioacuten
rhBMP2
20 ngml de
contasnte
liberacioacuten
sostenida
Mejor formacioacuten
oacutesea 8 semanas
despueacutes
Fu at al 2012
(126)
PLGA PVA 10-100
μm
rhBMP2-BSA
69 (BMP)
Burst (20 )
Prolongado
hasta un 77
(28 diacuteas)
Moleacuteculas de
BMP2 con
bioactividad
Tian et al
2012
(130)
PLGA 7525 PVA 182 μm 82 -
Buenos resultados
de reparacioacuten oacutesea
de 8 a 12 semanas
Rodriguez-
Evora et al
2014 (130)
PLGA PVA 228 μm 605
30 en la
descarga inicial
Liberacioacuten maacutes
lenta de un 4
por semana
Despueacutes de 8
semanas
liberado un
60
Sin peacuterdida de
bioactividad
Reyes et al
2013 (132)
PLGAPEG Sin siacutentesis de
doble emulsion
100-200
μm Adsorcioacuten BMP2
13 en la
descarga inicial
Liberacioacuten mas
lenta de 001-8
por dia
Despueacutes de 23
diacuteas liberado
un 70
Sustancial
regeneracioacuten oacutesea
debido al
ldquoandamiordquo
Rahman et al
2014 (181)
PLGA Diferente PVA 20-100
μm
30 (sin cubrir
PLGA)
90 (cubriendo
PLGA)
26-49 (1 dia)
Total 2 semanas
despueacutes
Sin peacuterdida de
bioactividad
Lupu-Haber
et al 2013
(134)
PLGA 7525 PVA 5-125 μm -
Descarga inicial
de un 30 (1
diacutea)
Prolongada 35
diacuteas
Mayores
voluacutemenes y
cobertura de
superficie del
Nuevo hueso
Wink et al
2014
(138)
PLGA Heparina 200-800
nm
Adsorcioacuten BMP2
94
Sin descarga
inicial
Prolongado 4
semanas
Reduccioacuten
significativa de la
dosis de BMP2
para una Buena
formacioacuten oacutesea
La et al 2010
(122)
PLGA Heparina-
poloxaacutemero 160 nm
Adsorcioacuten BMP2
100
Descarga inicial
(4-7) perfil
lineal
Mayor
mineralizacioacuten de
la matriz del hueso
regenerado
Chung et al
2007 (123)
PLGA Heparina 100-250
nm Adsorcioacuten 94
Descarga inicial
10 (1 dia)
60 30 diacuteas
despueacutes
Sin peacuterdida de
bioactividad
Eficacia de la
administracioacuten
cantidad 50 veces
menor
Jeon et al
2008 (124)
PLGA PVA ~ 300 nm 80 85 descarga
inicial (1 dia)
Sin peacuterdida de
bioactividad
Yilgor et al
2009 (127)
PLGA (en anillos) PVA 215 μm 66 Descarga El 60 de los Rodriguez-
52
15 Vectorizacioacuten Entrega dirigida
Las nano-esferas de PLGA representan un sistema de administracioacuten de biomoleacuteculas bien
estudiado que podriacutea aplicarse a la seleccioacuten celular con el fin de mejorar el suministro de
proteiacutenas especiacuteficas o aacutecidos nucleicos dentro o cerca de las ceacutelulas de referencia de ingenieriacutea
oacutesea es decir ceacutelulas madre mesenquimales (Vo Kasper and Mikos 2012) Las propiedades de
direccionamiento pueden ser suministradas por una estrategia de funcionalizacioacuten del ligando
modificacioacuten de la estructura superficial del nano-transportador conjugando un ligando especiacutefico
de ceacutelula para dirigir la liberacioacuten de biomoleacuteculas encapsuladas preferiblemente en estrecha
asociacioacuten con las ceacutelulas diana (Ji et al 2012) El uso de nanopartiacuteculas con una unioacuten covalente
de diferentes ligandos da lugar a una teacutecnica potencial para administrar biomoleacuteculas especiacuteficas
de ceacutelulas oacuteseas para la ingenieriacutea oacutesea (Luginbuehl et al 2004)
Los anticuerpos especiacuteficos que reconocen los receptores de superficie en estas ceacutelulas podriacutean
acoplarse covalentemente a la superficie de las nanopartiacuteculas de PLGA obteniendo inmuno-
nanopartiacuteculas Hay varios ejemplos de inmovilizacioacuten de anticuerpos en la superficie de
nanopartiacuteculas de PLGA Kocbek y colaboradores demostraron el reconocimiento especiacutefico de
las ceacutelulas tumorales de mama por un anticuerpo monoclonal especiacutefico unido a las nanopartiacuteculas
fluorescentes PLGA obtenidas mediante el proceso de emulsioacuten WOW (Kocbek et al 2007)
Para la unioacuten covalente de la superficie utilizaron un meacutetodo de carbodimida maacutes simple que
promueve la formacioacuten de un enlace amida entre los grupos terminales carboxiacutelicos libres de
nanopartiacuteculas de PLGA y los grupos amino primarios de la moleacutecula del anticuerpo (Ertl et al
2000) Este procedimiento puede verse muy influenciado por la presencia de estabilizadores
frecuentemente utilizados para conferir estabilidad coloidal a las nanopartiacuteculas La movilidad
electroforeacutetica de las nanopartiacuteculas de PLGA con un anticuerpo (inmuno-γ-globulina anti-
proteiacutena C-reactiva humana) unido covalentemente a la superficie como se muestra en la figura
5 Es necesario observar la draacutestica disminucioacuten en los valores de movilidad del anticuerpo
modificado con respecto a las nanopartiacuteculas de PLGA desnudas lo que podriacutea implicar una baja
53
estabilidad coloidal y la posterior agregacioacuten del nanosistema Santander y colaboradores
propusieron una menor carga de anticuerpos en la que las nanoparticulas de PLGA desnudas
deben ser recubiertas por un agente surfactante no ioacutenico con el fin de obtener nanopartiacuteculas
estables inmunorrectivas (Santander-Ortega Bastos-Gonzalez and Ortega-Vinuesa 2007)
Ratzinger y colaboradores indicaron que la presencia de altas concentraciones de poloxaacutemero
disminuyoacute la eficacia de acoplamiento a grupos carboxiacutelicos en nanopartiacuteculas de PLGA lo que
demuestra que es necesario un equilibrio que combine maacutes estabilidad y mejor eficacia de
acoplamiento (Ratzinger et al 2010) Para evitar este problema Cheng y colaboradores
sintetizaron el copoliacutemero en bloque de PLGA-PEG funcionalizado con carboxilo uniendo un
aptaacutemero especiacutefico a la superficie de las nanopartiacuteculas pegiladas mediante el meacutetodo de la
carbodiimida En este trabajo se ha demostrado una mejor administracioacuten de faacutermacos en los
tumores de proacutestata en comparacioacuten con las nanopartiacuteculas equivalentes no dirigidas (Cheng et
al 2007)
16 Ingenieriacutea tisular soportes 3D o ldquoscaffoldsrdquo
Los datos publicados en la literatura indican que las nanopartiacuteculas micropartiacuteculas de PLGA
prometen lograr una administracioacuten sostenida espacial y temporalmente controlada por factores
de crecimiento requeridos para el desarrollo celular y la diferenciacioacuten celular Se pueden
incorporar a ceacutelulas en scaffolds soacutelidos o hidrogeles inyectables (Danhier et al 2012) Los
scaffolds o andamios son estructuras 3D porosas que normalmente se utilizan para mejorar la
ingenieriacutea tisular oacutesea (A C Carreira et al 2014) De acuerdo con Tian y colaboradores (Tian et
al 2012) un scaffolds disentildeado con este objetivo debe tener (1) resistencia mecaacutenica apropiada
para soportar el crecimiento de hueso nuevo (2) porosidad apropiada para permitir el crecimiento
de las ceacutelulas relacionadas con los huesos (3) buena biocompatibilidad que permite el crecimiento
de ceacutelulas en su superficie sin ser rechazado por el cuerpo (4) baja toxicidad para las ceacutelulas y
tejidos de alrededor (5) ser capaz de inducir la diferenciacioacuten osteogeacutenica de las ceacutelulas madre
54
relacionadas con los huesos (6) ser biodegradable con productos de degradacioacuten no toacutexicos para
que eventualmente puedan ser reemplazados por nuevo hueso Ademaacutes el scaffolds para la
regeneracioacuten oacutesea debe mantener el suministro o la liberacioacuten de BMP (factores de crecimiento)
in situ durante un tiempo prolongado De esta forma las nano micropartiacuteculas dentro de los
scaffolds se utilizan para liberar un flujo adecuado de estas biomoleacuteculas de sentildealizacioacuten y
preservar su estructura funcional (Romagnoli DrsquoAsta and Brandi 2013) Para hacerlo se requiere
una liberacioacuten inicial del factor de crecimiento encapsulado en las primeras horas para poder
obtener raacutepidamente una concentracioacuten terapeacuteutica efectiva seguida de un perfil de liberacioacuten
sostenido a largo plazo (Puppi et al 2014) La mayoriacutea de las partiacuteculas polimeacutericas insertadas
en las estructuras de los scaffolds estaacuten en una escala micromeacutetrica El objetivo principal de estas
micropartiacuteculas es la proteccioacuten y el control temporal de la entrega del factor de crecimiento Sin
embargo dada la porosidad de estas estructuras las nanopartiacuteculas y especialmente las partiacuteculas
de algunas micras pueden volverse maacutes importantes ya que es posible disentildear sistemas con una
difusioacuten simple y faacutecil a traveacutes de la estructura Este proceso podriacutea permitir el reconocimiento
especiacutefico de un tipo de ceacutelula particular liberando sus BMP encapsuladas en el mismo entorno
y ayudando a su diferenciacioacuten al tejido celular oacuteseo
55
2 HIPOacuteTESIS
Lo que sabemos hasta ahora es que las BMPs y especiacuteficamente la BMP-2 son muy uacutetiles
para promover la regeneracioacuten oacutesea induciendo una mayor formacioacuten de hueso de la zona
receptora de igual calidad que el hueso nativo del paciente Sin embargo la administracioacuten local
presenta algunas limitaciones como que la proteiacutena se puede inactivar muy raacutepidamente y la
distribucioacuten de la BMP en una suspensioacuten liacutequida hace que sea imposible estar seguro de que la
proteiacutena haya alcanzado el objetivo Para ayudar a resolver estos problemas planteamos la
siguiente hipoacutetesis
HIPOTESIS CIERTA La encapsulacioacuten de BMP-2 con nuestras nanopartiacuteculas permiten una
administracioacuten localizada un transporte especifico y una liberacioacuten controlada de la biomoleacutecula
mejorando asiacute su farmacocineacutetica y farmacodinamia y disminuyendo los efectos secundarios
derivados de esta
HIPOacuteTESIS NULA Que tras la experimentacioacuten no seamos capaces de promover la
encapsulacioacuten de la BMP-2 dentro de nuestras nanopartiacuteculas de PLGA o que producieacutendose no
se produzca la administracioacuten de BMP-2 localizada y liberacioacuten prolongada o que nuestro sistema
de encapsulacioacuten pueda presentar efectos citotoacutexicos a nivel celular
56
3OBJETIVOS
31 Objetivo principal
Optimizar la formulacioacuten de diferentes tipos de nanopartiacuteculas de PLGA para el transporte y
suministro de BMP-2 que permita conseguir una cineacutetica de liberacioacuten controlada aumentando la
vida media de este factor de crecimiento oacuteseo y preservando su accioacuten bioloacutegica
32 Objetivos secundarios
Nos planteamos los siguientes
1 Llevar a cabo la siacutentesis de NPs polimeacutericas de PLGA mediante el procedimiento de
doble emulsioacuten para conseguir nanosistemas coloidal y temporalmente estables
2 Modelar los procedimientos de siacutentesis de NPs de PLGA para la encapsulacioacuten o carga
de moleacuteculas proteicas en la proporcioacuten adecuada sin alterar su actividad bioloacutegica
3 Desarrollar una completa caracterizacioacuten quiacutemico-fiacutesica de los diferentes nanosistemas
polimeacutericos en ausencia de proteiacutena y con carga de la misma haciendo hincapieacute en la
interaccioacuten surfactante-proteiacutena y analizando sus propiedades superficiales y
coloidales
4 Desarrollar una caracterizacioacuten bioloacutegica de los diferentes nanosistemas con proteiacutena
centrada en el anaacutelisis de la cineacutetica de liberacioacuten proteica y su actividad bioloacutegica
5 Evaluar in vitro las interacciones celulares citotoxicidad y captacioacuten celular de los
diferentes sistemas de NPs de PLGA en ceacutelulas estromales mesenquimales de hueso
alveolar humano
6 Tomando como punto de partida el modelo de NPs con proteiacutena con las mejores
propiedades coloidales y bioloacutegicas formular las condiciones oacuteptimas para obtener un
nanotransportador de partiacuteculas de PLGA cargado con BMP2
57
7 Desarrollar una completa caracterizacioacuten quiacutemico-fiacutesica y bioloacutegica de las NPs con
BMP2 analizando sus propiedades superficiales coloidales y la cineacutetica de liberacioacuten
proteica
8 Analizar la actividad bioloacutegica de la BMP-2 vehiculizada mediante las NPs de PLGA
mediante experiencias de proliferacioacuten migracioacuten y diferenciacioacuten osteogeacutenica en
ceacutelulas estromales mesenquimales de hueso alveolar humano
58
4NANOSISTEMA DE ENTREGA DOBLE PARA BIOMOLEacuteCULAS
FORMULACIOacuteN CARACTERIZACIOacuteN Y LIBERACIOacuteN IN
VITRO
41Antecedentes
La regeneracioacuten tisular es una accioacuten bioloacutegica compleja que implica muacuteltiples pasos de forma
secuencial ordenada y controlada (Padial-Molina et al 2012) (Padial-Molina Rodriguez et al
2015) Claacutesicamente se han propuesto moleacuteculas bioactivas para ayudar en estos procesos Sin
embargo el uso de altas dosis la desnaturalizacioacuten y la peacuterdida de actividad bioloacutegica el tiempo
de accioacuten descontrolado y la difusioacuten a otros tejidos destacan como los principales problemas de
esta estrategia terapeacuteutica (Ortega-Oller et al 2015) Para ayudar a resolver estas dificultades en
los uacuteltimos antildeos se ha investigado intensamente la nanomedicina como un aacuterea emergente Esto
implica meacutetodos de diagnoacutestico terapeacuteuticos y de regeneracioacuten mediante estructuras y sistemas
en los que el tamantildeo y la forma se controlan a nivel atoacutemico molecular y supramolecular (Ki-
Bum Lee Ani Solanki J Dongun Kim 2009) El transporte y la administracioacuten controlada de
faacutermacos yo biomoleacuteculas terapeacuteuticas mejora su farmacocineacutetica y farmacodinaacutemica y al
mismo tiempo minimiza los efectos secundarios nocivos Para estos propoacutesitos se describieron
diferentes tipos de nanosistemas El aacutecido polilaacutectico-co-glicoacutelico (PLGA) exhibe una baja
citotoxicidad asi como una alta biocompatibilidad y biodegradabilidad con las liberaciones de
subproductos no toacutexicos
En la uacuteltima deacutecada se ha investigado el uso de PLGA para administrar un amplio espectro de
agentes activos desde moleacuteculas de faacutermacos hidroacutefobas (Yallapu et al 2010) (Nair and Sharma
2012) (Shankarayan Kumar and Mishra 2013) a biomoleacuteculas hidroacutefilas como peacuteptidos
(Loureiro et al 2016) proteiacutenas (Blanco and Alonso 1998) (Perez De Jesus and Griebenow
2002) (Manuel J Santander-Ortega Csaba et al 2010) (Pirooznia et al 2012) (drsquoAngelo et al
2010) (White et al 2013) o aacutecidos nucleicos (Pantazis et al 2012) (Park et al 2013) Estos
59
sistemas de entrega se han producido a traveacutes de diferentes procesos de formulacioacuten para su
aplicacioacuten en terapias tanto sisteacutemicas como locales especiacuteficas del sitio (Wan and Yang 2016)
Sin embargo su disentildeo y desarrollo como nanotransportadores son difiacuteciles debido al patroacuten
de liberacioacuten problemaacutetico que presentan cuando las moleacuteculas encapsuladas son proteiacutenas para
las cuales las descargas iniciales y la liberacioacuten lenta o incompleta podriacutean ser un problema (Wan
and Yang 2016) (Giteau et al 2008) (Fredenberg et al 2011) Ademaacutes las condiciones
especiacuteficas de la liberacioacuten pueden necesitar ser diferentes dependiendo de la aplicacioacuten final del
nanotransportador (Fredenberg et al 2011) (Mohamed and van der Walle 2008)
La teacutecnica de doble emulsioacuten de aguaaceiteagua (WOW) es el meacutetodo de encapsulacioacuten de
proteiacutenas maacutes ampliamente utilizado para PLGA en micro (MP) y nanopartiacuteculas (NP) (Csaba et
al 2004) (Makadia and Siegel 2011) Permite modular diferentes factores como el tipo de PLGA
el uso de otros poliacutemeros mezclados con PLGA la adicioacuten de surfactantes el estreacutes mecaacutenico o
el solvente orgaacutenico (Fredenberg et al 2011) Tambieacuten es posible construir varios tipos de
copoliacutemeros para modificar la hidrofobicidad la relacioacuten de hidrofilicidad (Wan and Yang 2016)
(Danhier et al 2012) y la estabilidad el tamantildeo y el proceso de liberacioacuten coloidal El par PLGA
polietilenglicol y los surfactantes como el alcohol poliviniacutelico (PVA) o los oacutexidos de polietileno
(PEO) son los maacutes ampliamente estudiados (Nair and Sharma 2012) (Manuel J Santander-
Ortega Csaba et al 2010) (Ratzinger et al 2010) (Meng et al 2003)
Por otro lado la ingenieriacutea de tejidos requiere la participacioacuten de ceacutelulas estromales
mesenquimales (MSC) (Padial-Molina OrsquoValle et al 2015) Se sabe que las MSC tienen la
capacidad de diferenciarse en muacuteltiples tipos de ceacutelulas incluidos los osteoblastos Los
osteoblastos son las ceacutelulas principales responsables de sintetizar el tejido oacuteseo mineralizado Este
proceso estaacute regulado por entre otras moleacuteculas BMP-2 (Ortega-Oller et al 2015) Las
partiacuteculas de PLGA cargadas con BMP-2 son sistemas ampliamente utilizados como se ha
descrito y revisado por otros autores (Ortega-Oller et al 2015) (Yilgor Hasirci and Hasirci 2010)
(Li et al 2009) (Wang et al 2015) (Shim et al 2016)
60
Asiacute dentro de este contexto el objetivo del presente estudio fue optimizar la formulacioacuten y
propiedades de un sistema de nanopartiacuteculas con gran variedad de aplicaciones terapeacuteuticas
Probamos dos estrategias diferentes para obtener NP de tensioactivo PLGA utilizando lisozima
como modelo para BMP-2 Analizamos el tamantildeo y la morfologiacutea el iacutendice de polidespersidad
el potencial zeta la estabilidad coloidal y la eficiencia de encapsulacioacuten (EE) de la proteiacutena
Una vez finalizada la caracterizacioacuten fiacutesico-quiacutemica el estudio se centroacute en el proceso de
liberacioacuten de proteiacutenas utilizando diferentes teacutecnicas para estudiar los resultados de experimentos
in vitro y centraacutendose en el patroacuten de liberacioacuten y la actividad bioloacutegica de la lisozima liberada
De esta manera se establecioacute una nueva formulacioacuten para desarrollar un nanosistema de PLGA
con una distribucioacuten de tamantildeo dual singular y el equilibrio adecuado entre encapsulacioacuten y
liberacioacuten de proteiacutenas bioloacutegicamente activas Finalmente los efectos del sistema PLGA
propuesto se probaron en MSC primarias in vitro como prueba del nuevo sistema desarrollado
42Materiales y meacutetodos
421Formulacioacuten de las nanoparticulas
El aacutecido poli (laacutectico-co-glicoacutelico) (PLGA 5050) ([C2 H2 O2]x [C3 H4 O2]y) x = 50 y = 50
(Resomerreg 503H) 32 - 44 kDa se usoacute como poliacutemero El surfactante polimeacuterico Pluronicreg F68
(Poloxaacutemero 188) (Sigma-Aldrich) se usoacute como el emulsionante Su estructura se basa en un
copoliacutemero tri-bloque de poli (oacutexido de etileno) PEO y poli (oacutexido de propileno) PPO (PEO)a ndash
(PPO)b ndash (PEO)a con a = 75 y b = 30 La lisozima de huevo de gallina (Sigma-L7651) se usoacute
como proteiacutena hidroacutefila El agua se purificoacute en un sistema Milli-Q Academic de Millipore Se
desarrollaron dos meacutetodos de formulacioacuten diferentes denominados O-F68 y W-F68
En el meacutetodo O-F68 se disolvieron 25 mg de PLGA y 15 mg de F68 en 660 120583119871 de
diclorometano (DMC) y se agitaron en el vortex Luego se antildeadieron 330 micro-litros de acetona
y se agitaron en el vortex tambieacuten A continuacioacuten se antildeadieron 100 120583119871 de una solucioacuten
tamponada a pH 128 con o sin lisozima (5 mg mL) gota a gota mientras se agita en vortex
61
durante 30 s Inmediatamente esta emulsioacuten primaria de agua aceite (WO) se vertioacute en un vidrio
que conteniacutea 125 ml de etanol bajo agitacioacuten magneacutetica y se antildeadieron 125 ml de agua MilliQ
Despueacutes de 10 minutos de agitacioacuten magneacutetica los disolventes orgaacutenicos se extrajeron
raacutepidamente por evaporacioacuten al vaciacuteo hasta que la muestra alcanzoacute un volumen final de 10 ml
En el meacutetodo W-F68 se disolvieron 100 mg de PLGA en un tubo que conteniacutea 1 ml de acetato
de etilo (EA) y se agitaron en vortex Se antildeadieron 40 120583119871 de una solucioacuten tamponada a pH 128
con o sin lisozima (20 mg ml) e inmediatamente se sonicaron (Branson Ultrasonics 450 Analog
Sonifier) fijando el debido ciclo de trabajo al 20 y de control de salida a 4 durante 1 min con
el tubo rodeado de hielo Esta emulsioacuten primaria WO se vertioacute en un tubo de plaacutestico que conteniacutea
2 ml de una solucioacuten tamponada (pH 128) de F68 a 1 mg ml y se agitoacute en voacutertex durante 30 s
Luego el tubo rodeado de hielo se sonicoacute de nuevo a la maacutexima amplitud para la micro punta
(control de salida 7) durante 1 minuto Esta segunda emulsioacuten WOW se vertioacute en un vaso que
conteniacutea 10 ml de la solucioacuten tamponada de F68 y se mantuvo bajo agitacioacuten magneacutetica durante
2 min El disolvente orgaacutenico se extrajo luego raacutepidamente por evaporacioacuten al vaciacuteo hasta un
volumen final de 8 ml
422 Limpieza y almacenamiento
Despueacutes de la evaporacioacuten del disolvente orgaacutenico la muestra se centrifugoacute durante 10 min a
20 deg C a 14000 o 12000 rpm para los meacutetodos O-F68 y W-F68 respectivamente El sobrenadante
se filtroacute usando filtros de 100 nm para medir la proteiacutena no encapsulada libre El sedimento se
resuspendioacute luego en PB hasta un volumen final de 4 ml y se mantuvo en refrigeracioacuten a 4ordmC
1La carga de proteiacutenas y la eficiencia de encapsulacioacuten
La carga de proteiacutena inicial se optimizoacute para la formulacioacuten de nanopartiacuteculas preservando la
estabilidad coloidal final despueacutes del paso de evaporacioacuten y siendo diferente para cada
nanosistema Ademaacutes se usoacute 16 p p (Lys PLGA) para O-F68 y 08 p p (Lys PLGA)
para W-F68 La cantidad de lisozima encapsulada se calculoacute midiendo la diferencia entre la
cantidad inicial antildeadida y la proteiacutena libre no encapsulada que se analizoacute mediante el ensayo con
62
aacutecido bicinconiacutenico (BCA Sigma-Aldrich) Luego la eficacia de encapsulacioacuten de proteiacutenas (EE)
y la carga de faacutermaco final (DL) se calcularon de la siguiente manera
EE = 119872119868minus119872119865
119872119868119909 100 119863119871 =
119872119868minus119872119865
119872119868119901119900119897119894119898119890119903 119909 10
donde MI es la masa total inicial de Lys MF es la masa total de Lys en el sobrenadante acuoso
y Mpoliacutemero es la masa de PLGA en la formulacioacuten
423 Caracterizacioacuten de las nanoparticulas
2Caracterizacioacuten interfacial de la primera emulsioacuten agua en aceite
La tensioacuten superficial y las mediciones de reologiacutea dilatacional en la interfaz aire-agua se
realizaron en el OCTOPUS (Maldonado-Valderrama et al 2013) dispositivo de anaacutelisis de
superficies por gota pendiente con intercambio muacuteltiple de subfase (patente presentada
P201001588) descrito en detalle por Cabrerizo-Viacutelchez y col (Wege Holgado-Terriza and
Cabrerizo-Vilchez 2002) Aquiacute el aire juega el papel de la fase orgaacutenica La tensioacuten superficial
se calcula con el software DINATENreg basado en el anaacutelisis de forma de gota axisimeacutetrica
(ADSA) y el moacutedulo de dilatacioacuten (E) de la capa interfacial se determina a partir del anaacutelisis de
imagen con el programa CONTACTOreg
3Morfologiacutea de la partiacutecula
Las nanopartiacuteculas se obtuvieron por microscopiacutea electroacutenica de barrido (SEM) y microscopiacutea
electroacutenica de transmisioacuten de barrido (STEM) usando un microscopio electroacutenico de barrido de
emisioacuten de campo Zeiss SUPRA 40VP del Centro de Instrumentacioacuten Cientiacutefica de la Universidad
de Granada (CIC UGR)
4Tamantildeo de las nanoparticulas y movilidad electrocineacutetica
El diaacutemetro hidrodinaacutemico y la movilidad electroforeacutetica de las NP se determinaron usando un
dispositivo Zetasizer NanoZeta ZS (Malvern Instrument Ltd UK) que trabaja a 25 deg C con un
63
laacuteser He-Ne de 633 nm y un aacutengulo de dispersioacuten de 173 deg Cada punto de datos se tomoacute como un
promedio de tres mediciones de muestra independientes El tamantildeo de las NP se caracterizoacute por
escaacutener de luz dinaacutemica (DLS) Se calcularon el diaacutemetro hidrodinaacutemico promedio (media Z o
media acumulada) y el iacutendice de polidispersidad (PDI) Estos paraacutemetros se calculan a traveacutes de
un anaacutelisis acumulativo de los datos que es aplicable para las distribuciones de tamantildeo
monomodal estrecho (Hassan Rana and Verma 2015) Tambieacuten determinamos la distribucioacuten
del tamantildeo de intensidad a partir de un algoritmo proporcionado por el software Zetasizer
La movilidad electroforeacutetica se determinoacute mediante la teacutecnica de electroforesis laacuteser Doppler
Se establecioacute una distribucioacuten de movilidad electroforeacutetica asiacute como una movilidad
electroforeacutetica promedio para cada muestra (entendiendo por promedio dos veces seguidas para
cada una de las muestras)
La distribucioacuten del tamantildeo hidrodinaacutemico de las NP con distribuciones de tamantildeo amplio de
DLS tambieacuten se midioacute usando anaacutelisis de seguimiento de nanopartiacuteculas (NTA) en un NanoSight
LM10-HS (GB) FT14 (NanoSight Amesbury Reino Unido) Todas las muestras se midieron maacutes
de tres veces durante 60 s con ajuste manual del obturador ganancia brillo y umbral a 25 deg C La
distribucioacuten de tamantildeo promedio (concentracioacuten de partiacuteculas frente a diaacutemetro) se calculoacute como
un promedio de al menos tres independientes distribuciones del tamantildeo
5Resonancia magneacutetica nuclear (RMN) de las nanopartiacuteculas
El espectro de 1HNMR de F68 libre las partiacuteculas cargadas con lisozima del meacutetodo O-F68
con y sin F68 y las partiacuteculas cargadas con lisozima del meacutetodo W-F68 se midieron con un
espectroacutemetro VNMRS de 500 MHz (Agilent) en el Centro de Instrumentacioacuten Cientiacutefica (CIC)
de la Universidad de Granada
424 Estabilidad coloidal y temporal en biologiacutea media
Se midioacute el diaacutemetro hidrodinaacutemico promedio y el iacutendice de polidispersidad (PDI) por DLS de
cada sistema para determinar su estabilidad coloidal en diferentes medios (tampoacuten de fosfato
64
[PB] solucioacuten salina tamponada con fosfato [PBS] y medio de cultivo celular medio de Eagle
modificado de Dulbecco [DMEM] de Sigma) y en diferentes momentos despueacutes (0 1 y 5 diacuteas)
Los experimentos de liberacioacuten in vitro se realizaron siguiendo una metodologiacutea similar a la
descrita anteriormente (eficacia de encapsulacioacuten) pero utilizando 1 ml de cada muestra
suspendida en PBS a 37 C La proteiacutena liberada de estas muestras se determinoacute cada 24 horas
mediante anaacutelisis de sobrenadante y el sedimento se suspendioacute en el mismo volumen de tampoacuten
para mantener las condiciones de liberacioacuten Todos los experimentos fueron desarrollados por
triplicado
6Microscopia confocal
La lisozima se marcoacute con isotopo de fluoresceiacutena (FITC) usando un meacutetodo descrito por Kok
et al (Kok et al 1998) Despueacutes de la conjugacioacuten covalente de FITC y lisozima las
concentraciones se estimaron espectrofotomeacutetricamente utilizando los coeficientes de extincioacuten
descritos para FITC a 494 nm y 280 nm La concentracioacuten de lisozima se calculoacute midiendo la
absorbancia oacuteptica a 280 nm y restando la absorbancia FITC correspondiente a esta longitud de
onda Las imaacutegenes se realizaron en un microscopio confocal de escaneo laacuteser Nikon A1 de CIC
UGR Todos los experimentos se realizaron por triplicado y se replicaron al menos dos veces
425 Actividad bioloacutegica e interacciones
7Actividad bioloacutegica de la lisozima
La actividad bioloacutegica de la lisozima se analizoacute mediante un kit de actividad enzimaacutetica
(Sigma-Aldrich) utilizando ceacutelulas de Micrococcus lysodeikticus como sustrato siguiendo las
instrucciones del fabricante
8Captacioacuten celular
Se tomaron ceacutelulas madre mesenquimales humanas primarias (hMSC) del hueso alveolar
maxilar sano de acuerdo con protocolos descritos (Mason et al 2014) Despueacutes de confirmar su
fenotipo por pruebas de citometriacutea de flujo y diferenciacioacuten trilinaje 12000 ceacutelulas por pozo se
cultivaron en placas esteacuteriles con fondo de vidrio (Ibidi cat n 81158) durante la noche Estas
65
ceacutelulas fueron tratadas con medio sin suero fetal bovino (FBS) y guiacutea celular roja (1 5000)
(C34552 ThermoFisher) durante 30 min Entonces el medio fue eliminado y complementado con
10 de SFB despueacutes de lo cual se agregaron partiacuteculas con lisozima-FITC Entonces los hMSCs
eran incubadas 30 minutos nuevamente lavadas tres veces con PBS 1X y un suplementado medio
fresco con 2 de FBS agregado Finalmente las hMSCs fueron examinadas por un microscopio
confocal (Nikon Eclipse Ti-E) Cultivo celular en todos los casos se mantuvieron a 37 deg C y 5
de atmoacutesfera de CO
43 Resultados y discusioacuten
431 Formulacioacuten de las nanoparticulas
Los meacutetodos desarrollados en este trabajo estaacuten destinados a mejorar las teacutecnicas de
formulacioacuten existentes para las NP de PLGA cargadas de proteiacutenas hidrofiacutelicas basadas en un
proceso de doble emulsioacuten (Blanco and Alonso 1998) (Csaba et al 2004) La novedad de estos
meacutetodos es el uso del surfactante polimeacuterico F68 ya sea en la fase orgaacutenica (meacutetodo O-F68) o en
la fase acuosa (W-F68) Este surfactante reduce el tamantildeo de las NP mejora su estabilidad y
protege la proteiacutena encapsulada Ademaacutes la presencia de F68 en la superficie de las partiacuteculas
reduce el reconocimiento de los nanovehiacuteculos (nanotransportadores) por el sistema mononuclear
fagociacutetico (MPS) (Farace et al 2016)
Ademaacutes la eleccioacuten del solvente orgaacutenico afecta significativamente las propiedades del
sistema coloidal final ya que la solubilidad del solvente orgaacutenico regula la estructura interna y
superficial de la partiacutecula Ademaacutes la interaccioacuten del disolvente con la biomoleacutecula encapsulada
puede alterar su bioactividad como consecuencia de su desnaturalizacioacuten como se encontroacute para
el cloruro de metileno (Meng et al 2003) En el meacutetodo O-F68 se elige DMC como disolvente
orgaacutenico debido a su menor solubilidad en agua para facilitar el proceso de emulsificacioacuten y su
bajo punto de ebullicioacuten para facilitar la evaporacioacuten Sin embargo se antildeadioacute un solvente orgaacutenico
libremente miscible en agua (acetona) y el emulsionante F68 en esta fase orgaacutenica para reducir
66
sus efectos bioloacutegicos negativos sobre la proteiacutena encapsulada (Danhier et al 2012) Este
emulsionante tambieacuten reduce la interaccioacuten de la matriz de PLGA proteiacutena-hidrofoacutebica y por lo
tanto la interrupcioacuten de la estructura de la proteiacutena (Ortega-Oller et al 2015) Por el contrario
en el meacutetodo W-F68 se utilizoacute acetato de etilo como disolvente orgaacutenico que ejerce menos
efectos de desnaturalizacioacuten sobre la proteiacutena encapsulada (Sturesson and Carlfors 2000) La
mayor solubilidad en agua de este solvente favorece la eliminacioacuten raacutepida del solvente La
velocidad de eliminacioacuten del disolvente tambieacuten se acelera al aumentar la tensioacuten de cizallamiento
durante la segunda etapa de emulsificacioacuten Tambieacuten mejora la eficacia del encapsulamiento y
minimiza el tiempo de contacto entre la proteiacutena y el disolvente orgaacutenico (Ortega-Oller et al
2015) El poloxaacutemero F68 se introduce en la fase acuosa externa
Ambas formulaciones (O-F68 y W-F68) (Tabla 2) dieron lugar a muestras coloidalmente
estables y a la encapsulacioacuten de la lisozima dentro de las nanopartiacuteculas de acuerdo con el doble
meacutetodo de emulsioacuten WOW (Makadia and Siegel 2011)
67
PLGA
(mg)
F68
(mg)
LYSI
(mg)
Initial EE
LYSF
(mg)
DL
O-F68-Lys 25 15 04 16 625 025 1
W-F68-Lys 100 2 08 08 731 058 058
Tabla 2 Condiciones de formulacioacuten y resultados de encapsulacioacuten de proteiacutenas PLGA F68
y LYSI son la cantidad inicial de poliacutemero surfactante y lisozima respectivamente El inicial
es la tasa inicial de proteiacutena-poliacutemero en pesopeso EE es la eficiencia de encapsulacioacuten LYSF
es la cantidad final encapsulada de lisozima DL es la tasa final de carga del faacutermaco en
pesopeso
La lisozima fue elegida como una proteiacutena modelo debido a su bioestabilidad sus
caracteriacutesticas bien conocidas y su facilidad para cuantificar su actividad bioloacutegica (Lin et al
2007) (Cai et al 2008) Ademaacutes su tamantildeo molecular (143 kD) y su punto isoeleacutectrico baacutesico
(alrededor de pH = 11) lo convierten en un modelo apropiado para otras proteiacutenas como los
factores de crecimiento oacuteseo (White et al 2013) Tres objetivos principales impulsaron la
optimizacioacuten de la relacioacuten apropiada entre el poliacutemero el poloxaacutemero y la proteiacutena (1) para
tener nanosistemas coloides estables de tamantildeos submicromeacutetricos (2) encapsular una cantidad
suficiente de proteiacutena y (3) para prevenir la desestabilizacioacuten de proteiacutenas manteniendo su
actividad bioloacutegica
Por lo tanto independientemente del meacutetodo de formulacioacuten se pretendiacutea limitar la carga de
proteiacutena inicial para proporcionar nanosistemas estables coloidalmente En nuestro caso como se
muestra en la Tabla 2 los valores de iniciales fueron la mejor opcioacuten para mantener la
estabilidad coloidal sin cambiar significativamente la distribucioacuten del tamantildeo (ver a
continuacioacuten) En consecuencia DL presenta valores relativamente bajos para ambas
formulaciones aunque la cantidad encapsulada de lisozima LYSF es mayor que las requeridas
68
para proteiacutenas terapeacuteuticas con cantidades cliacutenicamente efectivas maacutes bajas (Paillard-Giteau et
al 2010) El valor de EE encontrado para las NP de O-F68-Lys estaacute en consonancia con las
caracteriacutesticas de la formulacioacuten y es similar a otros informes con diferentes proteiacutenas (Manuel J
Santander-Ortega Csaba et al 2010) (Blanco and Alonso 1998) (Santander-Ortega et al 2009)
(drsquoAngelo et al 2010) incluida la albuacutemina de suero bovino (BSA) o la insulina (Manuel J
Santander-Ortega Csaba et al 2010) (Santander-Ortega et al 2009) y varios factores de
crecimiento (drsquoAngelo et al 2010)
La presencia de surfactante estabiliza la emulsioacuten y reduce su tamantildeo Sin embargo tambieacuten
altera la interaccioacuten proteiacutena-poliacutemero lo que se traduce en una reduccioacuten de la eficacia de
encapsulacioacuten Esto fue evidenciado por Blanco y colaboradores al encapsular BSA y lisozima en
diferentes micropartiacuteculas de PLGA-poloxaacutemero (Blanco and Alonso 1998) Ademaacutes el tipo de
proteiacutena y su carga teoacuterica inicial son factores directamente relacionados con la EE y pueden
afectar la estabilidad coloidal de la emulsioacuten primaria como lo muestran Santander y
colaboradores (Manuel J Santander-Ortega Csaba et al 2010) La diferente relacioacuten poliacutemero
tensioactivo entre las dos formulaciones no es comparable ya que el tensioactivo se agrega de una
manera diferente En ambos casos utilizamos formulaciones anteriores como punto de partida
(Blanco and Alonso 1998) (Csaba et al 2004) y probamos varias relaciones poliacutemero
tensioactivo (datos no mostrados) con el fin de obtener la mejor estabilidad coloidal EE y DL
En la Tabla 2 mostramos los datos para las relaciones optimizadas de PLGA F68 en ambos
sistemas
En el meacutetodo W-F68 a pesar del mayor valor de EE con respecto al sistema O-F68 se esperaba
una encapsulacioacuten casi completa debido a la baja relacioacuten inicial de proteiacutena masa de PLGA
(Manuel J Santander-Ortega Csaba et al 2010) y a la ausencia de surfactante en la primera
emulsioacuten Las caracteriacutesticas del proceso de formulacioacuten modificado pueden tener la clave En
esta formulacioacuten la solubilidad relativamente alta del acetato de etilo en agua promueve la
difusioacuten raacutepida del disolvente orgaacutenico en la segunda fase acuosa Inicialmente se agrega un
69
pequentildeo volumen inicial de agua que contiene poloxaacutemero para evitar una precipitacioacuten raacutepida e
incontrolada del poliacutemero y para controlar la velocidad del proceso Esto se complementa
posteriormente con la adicioacuten de un volumen acuoso maacutes grande como se describioacute anteriormente
(Meng et al 2003) Cuando esta solidificacioacuten es lenta favorece el escape de la proteiacutena y la EE
disminuye Sin embargo si la solidificacioacuten es muy raacutepida el contacto de la proteiacutena con el
solvente orgaacutenico se minimiza y la EE aumenta En el lado negativo puede producir aglomeracioacuten
de poliacutemero que interfiere con la correcta formacioacuten de las NP La introduccioacuten de un paso
intermedio con un volumen reducido de fase acuosa con poloxaacutemero puede modular la velocidad
del proceso controlando la difusioacuten de acetato de etilo en el agua y permitiendo la difusioacuten en la
fase orgaacutenica del poloxaacutemero Una velocidad controlada del proceso de pre-solidificacioacuten del
poliacutemero en presencia de surfactante puede producir canales o poros en la cubierta polimeacuterica
que por un lado podriacutean facilitar la liberacioacuten de proteiacutena y por otro lado podriacutea reducir el valor
EE (Rosca Watari and Uo 2004) Como resultado de estos fenoacutemenos los DL finales (p p de la
lisozima poliacutemero) que se muestran en la Tabla 2 para ambos sistemas NP son adecuados para
su aplicacioacuten como sistemas de nanotransporte
432 Caracterizacioacuten de las Nanopartiacuteculas
9Caracterizacioacuten interfacial de la primera formulacioacuten de agua en aceite
Para obtener una mejor comprensioacuten del efecto del meacutetodo de formulacioacuten sobre las
propiedades interfaciales de la primera solucioacuten de agua (solucioacuten de lisozima) emulsioacuten en
aceite disentildeamos experimentos de superficie con lisozima y Pluronicreg F68 La diferencia
principal en los dos meacutetodos de formulacioacuten es coacutemo se agrega Pluronicreg F68 en fase acuosa
(W-F68) o en fase orgaacutenica (O-F68) Esta diferencia podriacutea afectar la composicioacuten de la superficie
de las NP y como resultado sus propiedades coloidales
La tensioacuten superficial y la elasticidad en el interfaz aire-agua fueron las propiedades analizadas
(Tabla 3) En esta interfaz las proteiacutenas cambian su conformacioacuten y exponen su parte hidrofoacutebica
al aire dependiendo de su estabilidad termodinaacutemica flexibilidad anfipaticidad tamantildeo
70
molecular y carga En nuestro caso la lisozima es una proteiacutena globular que se adsorbe en la
interfase aire-agua y forma una monocapa riacutegida debido a su estructura interna y la presencia y
cantidad de puentes disulfuro (Pezennec et al 2008) Nuestras mediciones se realizaron a pH 12
por lo tanto la lisozima estaacute cargada negativamente La Tabla 3 muestra la tensioacuten interfacial de
la monocapa de lisozima en la interfaz aire-agua despueacutes de 50 minutos de adsorcioacuten (457 plusmn 04
(mN m)) y su elasticidad (83 plusmn 4 (mN m)) La reduccioacuten de la tensioacuten interfacial en comparacioacuten
con la de la interfaz aire-agua (72 mN m) indica las caracteriacutesticas de tensioactivo de la lisozima
El alto valor de la elasticidad se debioacute a la carga y a las altas interacciones moleculares en la
monocapa de lisozima Cuando la monocapa se forma con Pluronicreg F68 la tensioacuten superficial
es ligeramente menor que con la lisozima cuando se agrega Pluronicreg en AP pero similar
(teniendo en cuenta el error) cuando se agrega en OP
Pluronicreg F68 es una moleacutecula desmontable hiacutebrida que se introduce en el interfaz aire-agua
cuando se disuelve en fase acuosa y tambieacuten cuando se deposita en la superficie de la partiacutecula
Se encuentran pequentildeas diferencias al comparar la tensioacuten superficial de la monocapa Pluronicreg
de los dos meacutetodos Los diferentes valores de tensioacuten interfacial alcanzados en ambos casos se
deben a los diferentes meacutetodos para agregar Pluronicreg F68 a la monocapa de lisozima formada
Pluronicreg F68 presenta una elasticidad menor que la lisozima como se esperaba ya que se sabe
que Pluronicreg F68 forma una monocapa flexible en la interfaz aire-agua (Torcello-Goacutemez et al
2011)
71
Primer Paso
Tensioacuten
Interfacial
(mNm)
Elasticidada
(mNm)
Segundo
Paso
Tensioacuten
Interfacial
(mNm)
Elasticidadb
(mNm)
Lisozima 457plusmn04 83plusmn4 - - -
Lisozima 457plusmn04 83plusmn4 - - -
Pluronicreg
F68 (AP) 421plusmn03 15plusmn3
Pluronicreg
F68 (AP) 379plusmn06 142plusmn05
Pluronicreg
F68 (OP) 475plusmn21 94plusmn05
Pluronicreg
F68 (OP) 38plusmn2 43plusmn4
Tabla 3 Tensioacuten interfacial y elasticidad dilatacional (a 1 Hz) de la interfase aire-agua (a)
despueacutes de adsorber lisozima o Pluronic F68 en la fase acuosa (AP) o Pluronicreg F68 en fase
orgaacutenica (OP) en el primer paso (b) cuando Pluronic F68 se agrega en AP u OP despueacutes de la
adsorcioacuten de la monocapa de lisozima (media plusmn sd n = 3)
Se disentildearon dos ensayos para imitar los meacutetodos de formulacioacuten de las partiacuteculas En el primer
ensayo (meacutetodo W-F68) se formoacute una monocapa de lisozima luego la mayor parte de la
partiacutecula se intercambioacute con la solucioacuten acuosa de Pluronicreg F68 y despueacutes de la adsorcioacuten se
midieron la tensioacuten interfacial y la elasticidad del interfaz (379 plusmn 06 mN my 142 plusmn 05 mN
m respectivamente) Este bajo valor de elasticidad fue muy similar al de la monocapa de
Pluronicreg F68 lo que indica que Pluronicreg F68 se encuentra en el interfaz adecuado y elimina
la lisozima previamente adsorbida En el segundo ensayo (meacutetodo O-F68) despueacutes de que se
formara la monocapa de lisozima Pluronicreg F68 disuelto en cloroformo se depositoacute sobre la
superficie de la partiacutecula El cloroformo se evapora raacutepidamente y se mide la tensioacuten interfacial y
la elasticidad del interfaz (38 plusmn 2 mN my 43 plusmn 4 mN m respectivamente) La elasticidad fue la
mitad de la de la monocapa de lisozima pura tal vez debido a la coexistencia de las moleacuteculas de
72
lisozima y Pluronicreg F68 en el interfaz La tensioacuten superficial del interfaz final no depende del
meacutetodo de adicioacuten de Pluronicreg pero es menor que la de la lisozima pura o que el Pluronicreg
puro
Dentro de este contexto se ha informado ampliamente que la adsorcioacuten de PEO y poloxaacutemeros
en el interfaz reduce la unioacuten a proteiacutenas (Manuel J Santander-Ortega Lozano-Loacutepez et al
2010) (Torcello-Goacutemez et al 2011) En el meacutetodo O-F68 la lisozima se expone al DCM despueacutes
de la formacioacuten de la primera emulsioacuten de agua en aceite incluso si se agrega Pluronicreg ya que
ambos coexisten en el interfaz En el meacutetodo W-F68 la proteiacutena estaraacute en contacto con el acetato
de etilo en este paso ya que el Pluronicreg estaacute ausente Sin embargo este disolvente tiene efectos
bioloacutegicos maacutes deacutebiles sobre la lisozima Pluronicreg podriacutea alcanzar el interfaz cuando se agrega
a la fase acuosa en el siguiente paso y desplazar la proteiacutena del interfaz que podriacutea difundirse
hacia afuera a la fase acuosa
10Morfologiacutea de la partiacutecula
La entrega la biodistribucioacuten y el mecanismo de accioacuten de un faacutermaco o biomoleacutecula
transportada dependen en gran medida del tamantildeo de la partiacutecula la concentracioacuten y el tiempo
(Penaloza et al 2017) En general la escala micromeacutetrica estaacute disentildeada para un suministro local
que permite la formacioacuten de reservorios de la moleacutecula transportada y minimiza la accioacuten del
sistema fagociacutetico (Schwendeman et al 2014) Sin embargo los sistemas nanomeacutetricos son maacutes
versaacutetiles porque permiten una distribucioacuten sisteacutemica son maacutes estables reactivos y permiten la
accioacuten extra e intracelular Este uacuteltimo mecanismo es esencial cuando la moleacutecula o el faacutermaco
debe actuar en el citoplasma (Wang et al 2012) o en cualquier otra estructura intracelular como
la mitocondria el aparato de Golgi el retiacuteculo endoplaacutesmico o el nuacutecleo (Penaloza et al 2017)
(Vasir and Labhasetwar 2007) (Yameen et al 2014) Tambieacuten se han investigado otros
paraacutemetros para alterar el destino intracelular de las partiacuteculas principalmente alterando la
decoracioacuten de su superficie (Sneh-Edri Likhtenshtein and Stepensky 2011) por ejemplo con
sentildeales de localizacioacuten nuclear (NLS) que utilizan el nuacutecleo como el objetivo de la partiacutecula (Vasir
73
and Labhasetwar 2007) Sin embargo estas estrategias auacuten se encuentran en fase de desarrollo
inicial (Penaloza et al 2017) (Yameen et al 2014)
Se buscoacute un tamantildeo de partiacutecula en la escala submicromeacutetrica (entre 2 y 500 nm) ya que es
necesario para la internalizacioacuten celular y una distribucioacuten raacutepida despueacutes de la administracioacuten
parenteral para alcanzar diferentes tejidos a traveacutes de diferentes barreras bioloacutegicas Las partiacuteculas
de menos de 200 nm minimizan su ingesta por macroacutefagos El tipo de disolvente orgaacutenico la
concentracioacuten del poliacutemero la adicioacuten de surfactante y la energiacutea de emulsioacuten controlan el tamantildeo
del sistema
El meacutetodo O-F68 da lugar a una distribucioacuten monomodal del tamantildeo de partiacuteculas con
diaacutemetros alrededor de 100 nm La adicioacuten de Pluronicreg F68 en la fase orgaacutenica refuerza la
estabilidad coloidal de la primera emulsioacuten y reduce el tamantildeo de partiacutecula en comparacioacuten con
las NP de PLGA en las que la estabilidad es puramente electrostaacutetica debido a los grupos
carboxiacutelicos del PLGA En el meacutetodo W-F68 se tienen en cuenta el esfuerzo cortante y el
volumen de la fase acuosa para producir un sistema con partiacuteculas de entre 100 y 500 nm
Las NP de O-F68-Lys tienen una forma esfeacuterica con una distribucioacuten de tamantildeo monomodal
(diaacutemetros alrededor de 100 nm) y una estructura nuacutecleo-capa (Fig 7a) Las partiacuteculas vaciacuteas
producidas con el meacutetodo O-F68 se muestran en las Figs 7 (sin F68) y Fig 8 (con F68) Tambieacuten
son esfeacutericas y con una estructura nuacutecleo-caparazoacuten pero un poco maacutes grande
W-F68-Lys NP tambieacuten presenta una forma esfeacuterica pero una distribucioacuten de tamantildeo
multimodal con diaacutemetros entre 140 y 450 nm la poblacioacuten maacutes grande estaacute alrededor de 260 nm
(Fig 7b) Tambieacuten se observa una estructura nuacutecleo-capa en estas partiacuteculas Las partiacuteculas vaciacuteas
del meacutetodo W-F68 se presentan en la Fig 9 correspondiente a un sistema maacutes polidisperso
74
(a) (b)
(c)
Figura 7 Morfologiacutea de las nanopartiacuteculas Micrografiacuteas de SEM (a b) y STEM (c) de
nanopartiacuteculas vaciacuteas utilizando el meacutetodo O-F68 sin F68
75
(a) (b)
(c) (d)
Figura 8 Micrografias de SEM (a b) y STEM (c d) de partiacuteculas vaciacuteas usando el meacutetodo O-
F68 con F68
76
(a) (b)
Figura 9 Micrografiacuteas de SEM de partiacuteculas vacias usando el meacutetodo W-F68
11Tamantildeo de las nanopartiacuteculas movilidad electrocineacutetica y estabilidad coloidal
La distribucioacuten del diaacutemetro hidrodinaacutemico de las partiacuteculas se determinoacute en primer lugar por
DLS La Tabla 4 contiene las principales propiedades coloidales de las partiacuteculas producidas con
los meacutetodos O-F68 y W-F68 vaciacuteas o cargadas con lisozima Los resultados de partiacuteculas vaciacuteas
del meacutetodo O-F68 pero sintetizados sin F68 tambieacuten estaacuten incluidos
Los paraacutemetros de tamantildeo se calcularon a traveacutes de un anaacutelisis acumulativo de los datos que
es aplicable para distribuciones de tamantildeo monomodal estrechas (Hassan Rana and Verma
2015) Las micrografiacuteas SEM y STEM indican que se podriacutea suponer tal aproximacioacuten para las
partiacuteculas del meacutetodo O-F68 pero no del W-F68 Por lo tanto las distribuciones de tamantildeo de
intensidad de los diferentes sistemas se muestran en la figura 11a La presencia de Pluronicreg F68
en el meacutetodo O-F68 reduce significativamente el tamantildeo y la polidispersidad de las NP Esto
concuerda con la reduccioacuten de la tensioacuten superficial cuando el F68 estaacute en el interfaz (Tabla 3)
lo que promueve el proceso de emulsioacuten Si las NP tambieacuten estaacuten cargadas con lisozima el tamantildeo
es auacuten menor pero la polidispersidad aumenta ligeramente en comparacioacuten con las partiacuteculas
vaciacuteas Las propiedades surfactantes de la lisozima se han demostrado con los resultados de la
tensioacuten superficial (Tabla 3)
La Fig 11a indica la presencia de partiacuteculas superiores a 500 nm con el W-F68 que no se
correlaciona con las micrografiacuteas SEM Por lo tanto se utilizoacute una teacutecnica diferente (NTA) para
77
obtener informacioacuten sobre la distribucioacuten del tamantildeo de dichos sistemas (figura 12b) Con NTA
la distribucioacuten de tamantildeos fue consistente con las imaacutegenes SEM Se encontraron distribuciones
de gran tamantildeo correspondientes a los sistemas multimodales con este meacutetodo pero la adicioacuten de
lisozima condujo a una clara reduccioacuten del tamantildeo Esto se debe a que la lisozima tambieacuten actuacutea
como emulsionante en la primera emulsioacuten
La carga electrocineacutetica de las NP se analizoacute midiendo la movilidad electroforeacutetica Para
comparacioacuten todas las muestras se midieron a pH 7 (tampoacuten de fosfato) En la Fig 12 se
presentan las distribuciones de movilidad electroforeacutetica mientras que los promedios
correspondientes se muestran en la Tabla 4
Las NP de PLGA normalmente estaacuten cargadas de forma negativa debido a los grupos
carboxiacutelicos del poliacutemero El uso de Pluronicreg F68 en el meacutetodo O-F68 reduce claramente la
movilidad electroforeacutetica de las NP lo que indica que algo de Pluronicreg estaacute ubicado en la
superficie de las NP Esta reduccioacuten se esperaba despueacutes de la incorporacioacuten de este tensioactivo
no ioacutenico al interfaz ya que la presencia de cadenas de oacutexido de polietileno causariacutea un
desplazamiento hacia afuera del plano de corte donde se define el potencial y esto disminuiriacutea
posteriormente la movilidad electroforeacutetica Los resultados previos para partiacuteculas de PLGA han
mostrado una reduccioacuten significativa directamente relacionada con el recubrimiento de
poloxaacutemero (Santander-Ortega et al 2006) Si comparamos los dos sistemas la superficie menos
negativa para las NP OF68 se relacionariacutea con una menor densidad del poliacutemero PLGA de
superficie llevando la carga eleacutectrica negativa a el interfaz Este resultado estariacutea en liacutenea con la
mayor cantidad de PLGA en la formulacioacuten del nanosistema WF68
78
Figura 10 Micrografiacuteas SEM y STEM de partiacuteculas cargadas de lisozima usando el meacutetodo
O-F68 (a) o W-F68 (b)
79
Media Z (nm) PDI Media-μ (micromcmVs)
Meacutetodo
O-F68
Vaciacuteo sin F68 266 plusmn 7 0293 -506 plusmn 015
Vaciacuteo 1627 plusmn 21 0081 -429 plusmn 018
Cargada de
Lisozima 1210 plusmn12 0244 -334 plusmn 007
Meacutetodo
W-F68
Vaciacuteo 273 plusmn 3 0193 -531 plusmn 011
Cargada de
Lisozima 293 plusmn 4 0169 -4212 plusmn 0013
Tabla 4 Propiedades coloidales de PLGA NP de diferentes meacutetodos de formulacioacuten Se
midieron en tampoacuten de fosfato (pH 7) El diaacutemetro hidrodinaacutemico promedio (media Z o media
acumulada) y el iacutendice de polidispersidad (PDI) se determinan a partir de DLS (Media plusmn sd n
= 3)
80
(a)
(b)
Figura 11 Distribucioacuten del diaacutemetro hidrodinaacutemico (a) mediante DLS a pH 7 (tampoacuten de
fosfato) de partiacuteculas de PLGA vaciacuteas y cargadas con lisozima de los meacutetodos O-F68 y W-F68
y (b) mediante NTA a pH 7 (tampoacuten de fosfato) de partiacuteculas de PLGA vaciacuteas y cargadas con
lisozima del meacutetodo W-F68
81
(a)
(b)
Figura 12 Distribucioacuten de movilidad electroforeacutetica a pH 7 (tampoacuten de fosfato) de partiacuteculas
de PLGA cargadas con lisozima y vaciacuteas a partir de los meacutetodos (a) O-F68 y (b) W-F68
82
Cuando la lisozima tambieacuten se utiliza en la siacutentesis la superficie es auacuten menos negativa lo que
podriacutea explicarse por la presencia de alguna proteiacutena (cuya carga neta es positiva) cerca o en el
interfaz Este uacuteltimo efecto tambieacuten se encuentra con el meacutetodo W-F68 La atractiva interaccioacuten
electrostaacutetica entre los residuos de aacutecido terminales negativos de PLGA y las moleacuteculas de
lisozima juega un papel clave en el proceso de encapsulacioacuten de proteiacutenas (Paillard-Giteau et al
2010) o adsorcioacuten (Cai et al 2008) en PLGA NPs que afecta la carga final de proteiacutenas
En relacioacuten con esta situacioacuten una caracteriacutestica importante de la formulacioacuten de
encapsulacioacuten W-F68 es que la fase acuosa tiene un pH de 12 lo que permite una carga neta
negativa de lisozima y por lo tanto evita la atraccioacuten electrostaacutetica de la proteiacutena y el poliacutemero
Esta situacioacuten puede reducir la eficacia de la encapsulacioacuten pero al mismo tiempo favorece el
posterior proceso de difusioacuten de proteiacutenas y en consecuencia la liberacioacuten a corto plazo
Estudios recientes han propuesto el uso de nanopartiacuteculas incrustadas en scaffolds impresos en
3D predisentildeados (Baumann et al 2017) (Lee et al 2017) lo que nos lleva a analizar la
estabilidad de las dos formulaciones en varios medios generalmente empleados durante la
preparacioacuten de otras estructuras Se encontraron distribuciones de tamantildeo similares al original
para las dos formulaciones en diferentes medios (PB PBS y DMEM) y en diferentes momentos
despueacutes de la siacutentesis (0 1 y 5 diacuteas) La carga eleacutectrica de los grupos terminales de aacutecido PLGA
y las moleacuteculas de poloxaacutemero ubicadas en la superficie NP confiere un mecanismo de estabilidad
coloidal combinado electrostaacutetico y esteacuterico como se ha descrito previamente (Manuel J
Santander-Ortega Lozano-Loacutepez et al 2010) (Santander-Ortega et al 2006) Ademaacutes las NPs
en todos los casos mantienen su tamantildeo almacenado a 4 deg C al menos durante 1 mes (datos no
mostrados) Por lo tanto los medios descritos podriacutean usarse potencialmente como medios de
almacenamiento o para preparar otras soluciones o scaffolds antes de colocarlos realmente en el
entorno vivo (in vitro o in vivo)
83
12NMR de las nanopartiacuteculas
En la Fig 12 tanto las NP vaciacuteas como las cargadas de proteiacutenas presentan una movilidad
electroforeacutetica menos negativa que las NP vaciacuteas sin F68 lo que podriacutea explicarse por la presencia
de Pluronicreg F68 en la superficie de la NP Al comparar los espectros 1HNMR de Pluronicreg F68
libre y las NP cargadas con lisozima de los meacutetodos O-F68 y W-F68 podemos verificar la
presencia de F68 en la superficie de las NP (figura 13) por los picos que se muestran entre 325 y
375 ppm y a 1 ppm Estos picos tambieacuten son visibles en los espectros de NP formulados con F68
(O-F68 y W-F68 figuras 14 y 15 respectivamente)
RMN de las nanopartiacuteculas
Figura 13 Espectro 1HMNR de F68 libre
84
Figura 14 Espectro de 1HMNR de partiacuteculas cargadas con lisozima del meacutetodo O-F68
Figura 15 Espectro de 1HMNR de partiacuteculas cargadas con lisozima del meacutetodo W-F68
433 Actividad bioloacutegica e interacciones
F68
F68
F68
F68
85
Una liberacioacuten controlada desde un sistema de administracioacuten basado en PLGA es una tarea
difiacutecil ya que depende de muacuteltiples factores el tipo de PLGA el solvente el estreacutes mecaacutenico el
uso de surfactantes etc (Hines and Kaplan 2013) La difusioacuten de la proteiacutena y la erosioacuten del
poliacutemero son los principales mecanismos implicados en la liberacioacuten de proteiacutena en los sistemas
de administracioacuten basados en PLGA Ademaacutes es tiacutepico encontrar una liberacioacuten en forma de una
raacutepida raacutefaga en la etapa inicial seguida de una fase de liberacioacuten lenta en un corto y mediano
plazo En esta fase las moleacuteculas de proteiacutena se difunden a traveacutes de la matriz del poliacutemero hasta
alcanzar una fase final en la que la degradacioacuten del poliacutemero por hidroacutelisis permite una liberacioacuten
maacutes raacutepida (Fredenberg et al 2011)
Por otro lado la liberacioacuten a corto plazo es de especial intereacutes para el transporte de factores de
crecimiento morfogeneacuteticos oacuteseos (BMP) Una explosioacuten inicial controlada seguida de una
liberacioacuten sostenida mejora significativamente la regeneracioacuten in vivo de hueso (Ortega-Oller et
al 2015) y cartiacutelago (Begam et al 2017) incluso en sistemas de liberacioacuten controlada doble
(Kim and Tabata 2015) Por estas razones centramos nuestro anaacutelisis en la liberacioacuten a corto
plazo teniendo en cuenta la reduccioacuten de la degradacioacuten del poliacutemero por hidroacutelisis encontrada
en sistemas similares para estos primeros pasos (Rescignano et al 2016)
86
Figura 16 Liberacioacuten acumulada (siacutembolos rellenos) y bioactividad residual (siacutembolos
abiertos) de O-F68-Lys (cuadrado) y W-F68-Lys (triaacutengulo) incubados durante diferentes
tiempos a 37 deg C en tampoacuten de fosfato salino (pH 74) (media plusmn sd n = 3)
La Fig 16 muestra la liberacioacuten acumulativa de lisozima de las NP de O-F68-Lys a corto plazo
(siete diacuteas) Estos resultados son consistentes con un proceso de dos pasos un estallido inicial y
uno de liberacioacuten lenta El primer paso podriacutea corresponder a la liberacioacuten de las moleacuteculas de
proteiacutena ubicadas cerca de la superficie cuya presencia se dedujo de los resultados de movilidad
electroforeacutetica (Fig 12) La segunda parte del proceso de liberacioacuten fue limitada y lenta debido a
la difusioacuten de proteiacutenas a traveacutes de la matriz de la cubierta polimeacuterica La interaccioacuten electrostaacutetica
especiacutefica entre las moleacuteculas de lisozima positiva y los grupos dxe aacutecido terminal negativo de
PLGA puede reducir la difusioacuten de proteiacutenas (Blanco and Alonso 1998) Cuando se agrega el
poloxaacutemero (F68) la interaccioacuten entre el surfactante y la proteiacutena ayuda al proceso de difusioacuten
lo que lleva a una liberacioacuten maacutes completa y sostenida (Manuel J Santander-Ortega Csaba et
87
al 2010) Tambieacuten ayuda a mantener la actividad bioloacutegica de la proteiacutena (Paillard-Giteau et al
2010) (Morille et al 2013) El poloxaacutemero reduce las interacciones proteiacutena-poliacutemero no
especiacuteficas (es decir interacciones hidroacutefobas) pero no las especiacuteficas (electrostaacutetica) por lo
tanto la difusioacuten a traveacutes de los poros llenos de agua o a traveacutes del poliacutemero sigue siendo limitada
En el estudio actual la fraccioacuten de proteiacutena liberada y el patroacuten de liberacioacuten son similares a los
encontrados en la literatura para la lisozima encapsulada en nano y micropartiacuteculas de mezclas de
PLGA y otros poliacutemeros o surfactantes (Meng et al 2003) (White et al 2013) (Perez De Jesus
and Griebenow 2002)
La curva de liberacioacuten de proteiacutenas de W-F68-Lys NP (Fig 16) revela que la tasa de
administracioacuten inicial es ideacutentica a la del sistema O-F68 lo que podriacutea significar una proporcioacuten
similar de proteiacutena encapsulada cerca o en la superficie para ambos sistemas NP Esto estariacutea de
acuerdo con la disminucioacuten anaacuteloga en la movilidad electroforeacutetica de las NP cargadas con
lisozima de la que informaron previamente (Fig 12) En la segunda parte del proceso la
interaccioacuten especiacutefica entre la proteiacutena y el poliacutemero estaacute nuevamente presente Sin embargo el
proceso de difusioacuten en el sistema W-F68 parece mejorar permitiendo una liberacioacuten continua y
sostenida despueacutes del estallido inicial y alcanzando un valor ligeramente maacutes alto para el tiempo
de liberacioacuten maacuteximo estudiado Este resultado podriacutea estar relacionado con la estructura interna
de la capa de poliacutemero que permite una mejor hidratacioacuten y por lo tanto una mejor difusioacuten de
la proteiacutena hacia el exterior Se ha informado previamente que el uso de disolventes orgaacutenicos
menos polares tales como DCM para formulaciones de partiacuteculas de PLGA aumenta la densidad
de la matriz de poliacutemero en comparacioacuten con disolventes orgaacutenicos maacutes polares tales como EA
Las matrices PLGA resultan maacutes resistentes en el primer caso pero reducen al mismo tiempo su
conectividad y difusioacuten (Bohr et al 2015) Meng y colaboradores (Meng et al 2003) encontraron
que una eliminacioacuten maacutes raacutepida de EA da como resultado una liberacioacuten cineacutetica maacutes lenta de la
proteiacutena debido a una disminucioacuten en la porosidad de las NP En cuanto al papel de Pluronicreg
Rafati y colaboradores (Rafati et al 2012) encontraron una mayor concentracioacuten de proteiacutena
88
encapsulada en los poros superficiales en micropartiacuteculas sintetizadas en presencia de surfactante
en la segunda fase acuosa de la emulsioacuten Dado que se introdujo un paso intermedio en nuestra
formulacioacuten W-F68 en la segunda fase acuosa de la emulsioacuten la eliminacioacuten de la EA por difusioacuten
se controloacute fuertemente de modo que se esperaba que la porosidad de estas NP aumentara Esta
porosidad mejora la difusioacuten de proteiacutenas lo que permite un patroacuten de liberacioacuten maacutes estable de
acuerdo con el resultado experimental encontrado para este sistema A pesar del efecto
desfavorable de la interaccioacuten proteiacutena-poliacutemero electrostaacutetico especiacutefico en la liberacioacuten la
cantidad de proteiacutena liberada en nuestras NP es sustancial lo que significa que hay otras
interacciones inespeciacuteficas que pueden ser moduladas por la presencia de surfactante permitiendo
un lanzamiento sostenido La cantidad de lisozima liberada es similar a la encontrada con la
lisozima fiacutesicamente adsorbida en la superficie de las nanopartiacuteculas de PLGA a pesar de la
atraccioacuten electrostaacutetica (Cai et al 2008) Ademaacutes de otras interacciones inespeciacuteficas la
concentracioacuten de electrolitos en el medio de liberacioacuten podriacutea modular esta atraccioacuten
electrostaacutetica entre la proteiacutena y el poliacutemero disminuyeacutendola y facilitando el proceso de
liberacioacuten (Manuel J Santander-Ortega Lozano-Loacutepez et al 2010)
Otro paraacutemetro notable es la actividad bioloacutegica de la liberacioacuten in vitro de lisozima que se
muestra en la Fig 16 Mientras que en el sistema O-F68 la bioactividad se reduce parcialmente
hasta en un 40 la proteiacutena suministrada por el sistema W-F68 mantiene la actividad por encima
del 90 con respecto a la de la lisozima suministrada comercialmente y se resuspende en el mismo
tampoacuten de liberacioacuten Como se discutioacute anteriormente tanto el solvente orgaacutenico como la
interaccioacuten hidrofoacutebica entre la proteiacutena y el poliacutemero a menudo causan la desnaturalizacioacuten de
las proteiacutenas encapsuladas (Paillard-Giteau et al 2010) (Gaudana et al 2013) Perez y
colaboradores (Perez De Jesus and Griebenow 2002) describen una peacuterdida parcial de actividad
cuando se usa DCM y una solucioacuten acuosa de PVA en la segunda etapa de emulsioacuten sin ninguacuten
excipiente adicional El uso de poloxaacutemeros en la formulacioacuten reduce tales interacciones mejora
la estabilidad de la proteiacutena y mantiene una capa acuosa que retiene las moleacuteculas de agua
89
necesarias para la funcioacuten bioloacutegica de la proteiacutena al mismo tiempo que ayuda a su difusioacuten Esta
situacioacuten junto con el uso de un solvente orgaacutenico deacutebil como EA ayuda a preservar la actividad
bioloacutegica de la lisozima como se encuentra para el sistema W-F68-Lys
La Fig 17 presenta diferentes imaacutegenes de microscopiacutea confocal relacionadas con el proceso
de liberacioacuten de NPs W-F68 cargadas de lisozima Una disminucioacuten en la intensidad de
fluorescencia fue apreciable a lo largo del experimento in vitro Ademaacutes la agregacioacuten del sistema
es visible a medida que avanza el proceso de incubacioacuten El anaacutelisis de estas imaacutegenes es
consistente con los resultados informados previamente para este sistema NP
90
Figura 17 Actividad bioloacutegica e interacciones Imaacutegenes octogonales obtenidas por
microscopiacutea confocal de Lisozima-FITC encapsulado en NPs W-F68 tras su incubacioacuten a
diferentes tiempos en tampon fosfato salino (pH 74) a 37ordmC Las NP se centrifugaron previamente
para eliminar la proteiacutena marcada liberada (a) Experimentos previos al lanzamiento (b)
despueacutes de 24h (c) despueacutes de 72h (d) despueacutes de 168h
13Captacioacuten celular
La captacioacuten celular de NP de PLGA es un proceso conocido que se ve afectado principalmente
por las propiedades de superficie la funcionalizacioacuten (Loureiro et al 2016) y la agregacioacuten de
partiacuteculas (Xiong et al 2011) La internalizacioacuten y el procesamiento intracelular posterior de las
partiacuteculas se ha descrito como un proceso activo por lo tanto depende de la energiacutea y puede
verse afectado por otros factores que alteran la absorcioacuten de energiacutea por parte de las ceacutelulas como
la temperatura (Penaloza et al 2017) Las partiacuteculas pueden internalizarse mediante varios
meacutetodos de endocitosis que dependen principalmente del tamantildeo de la partiacutecula partiacuteculas
(a) (b)
(c) (d)
91
dependientes de caveolina (diaacutemetro asymp 60 nm) independientes de clatrina (diaacutemetro asymp 90 nm) y
dependientes de clatrina (diaacutemetro asymp 120 nm) (Vasir and Labhasetwar 2007) (Yameen et al
2014) Una vez internalizados alrededor del 65 se exportan al espacio extracelular antes de
liberar cualquiera de sus contenidos mientras que el resto libera lentamente la moleacutecula
encapsulada en el espacio intracelular (Panyam and Labhasetwar 2003)
Figura 18 Proyeccioacuten z de 5 imaacutegenes de hMSCs visualizadas 30 minutos despueacutes de la
incubacioacuten con W-F68-LysFITCNPsorO-F68-LysFITCNPs Las hMSC se marcaron previamente
con Guiacutea celular roja Barra de escala 20 m
El proceso de liberacioacuten intracelular se ve afectado por la formulacioacuten de las partiacuteculas
(Penaloza et al 2017) Hemos demostrado que los sistemas propuestos siguen un patroacuten similar
a otros publicados anteriormente En un periodo de tiempo tan corto como 30 minutos despueacutes
de la incubacioacuten las NPs de W-F68-LysFITC fueron absorbidas por las ceacutelulas (Fig 18) Algunas
partiacuteculas W-F68 todaviacutea estaban en el medio por lo que la actividad dual podriacutea ocurrir Por el
92
contrario las NP de O-F68-LysFITC se vieron afectadas por la agregacioacuten y por lo tanto no
alcanzaron adecuadamente el espacio intracelular (Fig 18 para las imaacutegenes del eje Z veacutease la
Fig 19)
Superposicioacuten Canal Verde Canal Rojo P
royec
cioacuten z
93
Superposicioacuten Canal Verde Canal Rojo
Pro
yec
cioacuten z
94
Figura 19 Proyeccioacuten z de 5 imaacutegenes e imaacutegenes z independientes de hMSC visualizadas 30
minutos despueacutes de la incubacioacuten sin partiacuteculas y con NP W-F68-LysFITC o NP O-F68-LysFITC
Las hMSC se marcaron previamente con un rastreador celular rojo Barra de escala 20 microm
Pro
yec
cioacuten z
Superposicioacuten Canal Verde Canal Rojo
95
Esto contradice los anaacutelisis previos de la estabilidad coloidal en PB PBS y DMEM Este
hallazgo puede explicarse por el hecho de que aunque el medio de cultivo fue DMEM este uacuteltimo
medio se complementoacute con suero fetal bovino y las ceacutelulas liberan muchos factores al medio
extracelular que pueden afectar a este tipo de partiacuteculas Ninguno de los sistemas demostroacute ser
toacutexico para las ceacutelulas (Fig 20) No hay estudios disponibles que hayan informado alguacuten efecto
de la lisozima sobre las hMSC
Figura 20 Pruebas de viabilidad a las 6 horas de antildeadir diferentes dosis de partiacuteculas A
continuacioacuten se separaron las ceacutelulas se tintildeeron con azul tripaacuten y se contaron las ceacutelulas vivas
o muertas El graacutefico representa ceacutelulas vivas normalizadas y la desviacioacuten estaacutendar de tres
experimentos No se encuentran diferencias
0
02
04
06
08
1
12
14
16
Control W-F68
10microlml
W-F68
5microlml
O-F68
10microlml
O-F68 5microlml
Viability after 6 hours
Viabilidad 6 horas despueacutes
96
5FORMULACIOacuteN CARACTERIZACIOacuteN COLOIDAL Y EFECTO
BIOLOacuteGICO IN VITRO DE NANOPARTIacuteCULAS DE PLGA
CARGADAS CON BMP-2 PARA LA REGENERACIOacuteN OacuteSEA
51 Antecedentes
En el contexto de la nanomedicina la regeneracioacuten de tejidos usando micro y nano estructuras
coloidales que tienen un tamantildeo y actividad superficial uacutenicos ha recibido una atencioacuten creciente
en los uacuteltimos antildeos Se han realizado muchos esfuerzos para mejorar la ingenieriacutea de estos nano-
sistemas con el fin de alcanzar una entrega inteligente de moleacuteculas bioactivas para optimizar
sus ventajas terapeacuteuticas y minimizar los efectos secundarios nocivos (van Rijt and Habibovic
2017) Con este objetivo hay descrito un amplio espectro de nanoportadores biocompatibles que
muestran propiedades adecuadas para diferentes aplicaciones bioloacutegicas y terapeacuteuticas (Kumar et
al 2017) Entre estas variadas propuestas los nanosistemas polimeacutericos representan un grupo
importante siendo el PLGA uno de los maacutes utilizados debido a las propiedades comentadas
anteriormente a lo largo de este trabajo entre ellas destaca su biocompatibilidad
biodegradabilidad y baja citotoxicidad asi como su capacidad para administrar una amplia
variedad de moleacuteculas y faacutermacos activos moleacuteculas sinteacuteticas o naturales con propiedades
hidrofiacutelicas o hidrofoacutebicas y biomoleacuteculas de proteiacutenas a aacutecidos nucleicos (Danhier et al 2012)
(Ding and Zhu 2018) (Arias et al 2015) obteniendo la aprobacioacuten de diferentes agencias
farmaceacuteuticas para uso humano (Mir Ahmed and Rehman 2017) (Jana and Jana 2017)
Dentro de la ingenieriacutea de tejidos en el campo de la odontologiacutea las teacutecnicas de regeneracioacuten
oacutesea empiezan a despertar un gran intereacutes por parte de los diferentes profesionales de la salud
contribuyendo con ello a un mayor desarrollo de estas liacuteneas de investigacioacuten y generando una
mayor tendencia a diferentes viacuteas de crecimiento relacionadas con el subsanamiento de los
obstaacuteculos que pueden originarse durante el proceso de encapsulacioacuten de moleacuteculas hidrofilicas
o en el de funcionalizacioacuten especiacutefica de la superficie a fin de mejorar la versatilidad de las
diferentes moleculas supuestos eacutestos en los que el PLGA es el poliacutemero de referencia para la
97
comunidad cientiacutefica en aras de crear NP para favorecer la cicatrizacioacuten oacutesea (Bapat et al 2019)
La literatura describe el suministro de moleacuteculas bioactivas normalmente factores de crecimiento
utilizando micropartiacuteculas polimeacutericas (MP) y NP con PLGA como componente principal
(Ortega-Oller et al 2015) Entre los factores de crecimiento morfogeneacutetico oacuteseo la BMP-2
(proteiacutena morfogeneacutetica oacutesea 2) ha sido la maacutes citada con muchos ejemplos en los que la
encapsulacioacuten o la adsorcioacuten en la superficie permite una eficiencia de atrapamiento adecuada y
diversos patrones de liberacioacuten (Ji et al 2010) (Kirby et al 2011) (Qutachi Shakesheff and
Buttery 2013) (Wang et al 2015) (Zhang et al 2016) Para las proteiacutenas con una vida media
muy corta como las BMP los nanosistemas PLGA biodegradables proporcionan proteccioacuten y
una dosis oacuteptima para una estimulacioacuten adecuada de la diferenciacioacuten celular (Begam et al 2017)
(Balmayor et al 2009)
Por lo tanto dentro de este escenario en el presente trabajo buscamos optimizar un sistema
de nanopartiacuteculas para llevar a cabo y controlar la liberacioacuten de BMP-2 utilizando como punto de
partida el procedimiento de siacutentesis de un sistema de NP cargado de lisozima previamente
descrito para la encapsulacioacuten de ese modelo de proteiacutena (Ortega-Oller et al 2017) Ademaacutes
para encapsular BMP-2 preparamos un segundo sistema en el que esta proteiacutena se co-adsorbioacute
con albuacutemina de suero bovino en la superficie de NP vaciacuteas Hemos estudiado el tamantildeo y la
morfologiacutea la eficiencia de la encapsulacioacuten de proteiacutenas las caracteriacutesticas de la superficie y la
estabilidad coloidal y temporal para completar la caracterizacioacuten fisicoquiacutemica de ambos sistemas
NP
El perfil de liberacioacuten de BMP-2 indica el potencial de un nanoportador PLGA para la
regeneracioacuten oacutesea y depende en gran medida de la degradacioacuten del poliacutemero por hidroacutelisis (Xu et
al 2017) Sin embargo a corto plazo durante el cual la liberacioacuten no depende de esta degradacioacuten
quiacutemica es necesario un control adecuado de la liberacioacuten para modular otros procesos fiacutesicos
Por lo tanto enfocamos nuestros experimentos de liberacioacuten a corto plazo utilizando diferentes
teacutecnicas para comparar las dos muestras de NP y establecer los perfiles de liberacioacuten de BMP-2
98
correspondientes Finalmente la actividad bioloacutegica (migracioacuten celular proliferacioacuten y
diferenciacioacuten osteogeacutenica) se proboacute in vitro utilizando ceacutelulas estromales mesenquimales (MSC)
derivadas del hueso alveolar (Padial-Molina et al 2019)
52 Materiales y Meacutetodos
521Siacutentesis de nanoparticulas
14Formulacioacuten
El aacutecido poli (lactico-co-glicolico) (PLGA 5050) ([C2 H2 O2]x [C3 H4 O2]y) x = 50 y = 50
(Resomerreg 503H) 32 - 44 kDa se usoacute como poliacutemero El surfactante polimeacuterico Pluronicreg F68
(Poloxaacutemero 188) (Sigma-Aldrich) se usoacute como el emulsionante Su estructura se basa en un
copoliacutemero tri-bloque de poli (oacutexido de etileno) PEO y poli (oacutexido de propileno) PPO (PEO)a ndash
(PPO)b ndash (PEO)a con a = 75 y b = 30 La lisozima de huevo de gallina (Sigma-L7651) se usoacute
como proteiacutena hidroacutefila La proteiacutena morfogeneacutetica oacutesea recombinante humana rhBMP-2
(Sigma-H4791) se utilizoacute como biomoleacutecula terapeacuteutica El agua se purificoacute en un sistema Milli-
Q Academic Millipore Se usoacute un meacutetodo de siacutentesis de doble emulsioacuten siguiendo un
procedimiento previamente descrito con ligeras modificaciones (Ortega-Oller et al 2017) En
este meacutetodo se disolvieron 100 mg de PLGA y 3 mg de aacutecido desoxicoacutelico (DC) en un tubo que
conteniacutea 1 ml de acetato de etilo (EA) y se sometieron a voacutertice En total se agregaron 40 μL de
una solucioacuten tamponada a pH 128 con o sin rhBMP-2 (200 μg mL) y se sonicoacute de inmediato
(Branson Ultrasonics 450 Analifier Sonifier) durante 1 min (Dial del ciclo de trabajo 20 Dial
de control de salida 4) con el tubo rodeado de hielo Esta emulsioacuten primaria de WO se vertioacute en
un tubo de plaacutestico que conteniacutea 2 ml de una solucioacuten tamponada (pH 12) de F68 a 1 mg ml y
se agitoacute en voacutertex durante 30 s Luego el tubo rodeado de hielo se sonicoacute a la amplitud maacutexima
de la micro punta durante 1 minuto (control de salida 7) Esta segunda emulsioacuten WOW se vertioacute
en un vaso que conteniacutea 10 ml de la solucioacuten F68 tamponada y se mantuvo bajo agitacioacuten
magneacutetica durante 2 minutos El disolvente orgaacutenico se extrajo raacutepidamente por evaporacioacuten al
99
vaciacuteo hasta un volumen final de 8 ml Los sistemas de NP encapsulados vaciacuteos y BMP-2
resultantes se denominaron NP y NP-BMP2 respectivamente En la Figura 21 se muestra un
esquema detallado del procedimiento de siacutentesis con un rendimiento basado en el componente
PLGA siempre superior al 85
15Limpieza y almacenamiento
Despueacutes de la evaporacioacuten del solvente orgaacutenico la muestra se centrifugoacute durante 10 minutos
a 20 deg C a 12000 rpm El sobrenadante se filtroacute usando nanofiltros Millipore 01 μm para medir
la proteiacutena libre no encapsulada El sedimento se resuspendioacute en tampoacuten fosfato (NaH2PO4 115
mM) PB hasta un volumen final de 4 ml y se mantuvo refrigerado a 4ordmC En estas condiciones
los sistemas mantuvieron la estabilidad coloidal al menos durante un mes
16Carga de proteiacutenas y eficiencia de encapsulacioacuten
La carga inicial de proteiacutenas se optimizoacute para la formulacioacuten de nanopartiacuteculas preservando
la estabilidad coloidal final despueacutes de la etapa de evaporacioacuten y teniendo en cuenta las cantidades
mostradas en la literatura para este factor de crecimiento cuando se encapsula dentro de NPs de
PLGA (drsquoAngelo et al 2010) (Chang et al 2017) Por lo tanto elegimos 2 μg como la masa total
inicial de rhBMP-2 lo que significa una relacioacuten de 2 10 5 p p (rhBMP-2 PLGA) La
cantidad de rhBMP-2 encapsulado se calculoacute midiendo la diferencia entre la cantidad agregada
inicial y la proteiacutena libre no encapsulada presente en el sobrenadante despueacutes de la etapa de
limpieza que se proboacute mediante un ensayo inmuno-absorbente ligado a enzimas especiacuteficas
siguiendo las instrucciones del fabricante (ELISA kit RAB0028 de Sigma-Aldrich St Louis
MO EE UU) Luego la eficiencia de encapsulacioacuten de proteiacutenas (EE) se calculoacute de la siguiente
manera
EE = 119872119868minus119872119865
119872119868119909 100
donde MI es la masa total inicial de rhBMP-2 y MF es la masa total de rhBMP-2 en el
sobrenadante acuoso
100
17Adsorcioacuten Fiacutesica de Proteiacutenas
La albuacutemina de suero bovino (BSA) y la rhBMP-2 se acoplaron en la superficie de
nanopartiacuteculas vaciacuteas mediante un meacutetodo de adsorcioacuten fiacutesica El volumen apropiado de una
solucioacuten de proteiacutena acuosa que conteniacutea 05 mg de BSA y 2 μg de rhBMP-2 se mezcloacute con 5 ml
de tampoacuten de acetato (pH 5) que conteniacutea NP vaciacuteas con 125 mg de PLGA Esto proporcionoacute
una cantidad inicial de proteiacutenas correspondiente al 004 p p (proteiacutena PLGA) mientras que
la relacioacuten de masa entre proteiacutenas fue de 04 p p (rhBMP-2 BSA) Esta solucioacuten se incuboacute a
temperatura ambiente durante 2 h con agitacioacuten mecaacutenica Las nanopartiacuteculas se separaron de la
solucioacuten de tampoacuten por centrifugacioacuten y despueacutes de que se filtraran los sobrenadantes
(nanofiltros Millipore 01 μm) se analizaron cualitativamente por electroforesis en gel mientras
que la cuantificacioacuten de proteiacutenas se realizoacute mediante un ensayo de proteiacutena de aacutecido
bicinconiacutenico (BCA) (Sigma-Aldrich St Louis MO EE UU) Para BSA y el ELISA especiacutefico
para rhBMP-2 El sedimento de nanopartiacuteculas se resuspendioacute en tampoacuten fosfato (pH 74) y se
almacenoacute a 4ordmC Este sistema se denominoacute NP-BSA-BMP2
18Separacioacuten de proteiacutenas por electroforesis en gel SDS-PAGE
Las NP cargadas de proteiacutena y los diferentes sobrenadantes se trataron a 90 deg C durante 10
minutos en el siguiente tampoacuten Tris-HCl 625 mM (pH 68 a 25 deg C) dodecil sulfato de sodio al
2 (p v) (SDS) 10 de glicerol 001 (p v) de azul de bromofenol ditiotreitol (DTT) 40
mM Las muestras se separaron luego por tamantildeo en gel de poliacrilamida poroso al 12
(electroforesis en gel de poliacrilamida SDS 1D) bajo el efecto de un campo eleacutectrico La
electroforesis se realizoacute a voltaje constante (130 V 45 min) y los geles se tintildeeron usando una
solucioacuten de azul de Coomassie (01 de azul brillante de Coomassie R-250 50 de metanol y
10 de aacutecido aceacutetico glacial) y se destintildeeron con la misma solucioacuten que carece del tinte
101
522 Caracterizacioacuten de nanopartiacuteculas morfologiacutea tamantildeo concentracioacuten y movilidad
electrocineacutetica
Se obtuvieron imaacutegenes de NP mediante microscopiacutea electroacutenica de barrido (SEM) con un
microscopio electroacutenico de barrido de emisioacuten de campo SUPRA 40VP Zeiss del Centro de
Instrumentacioacuten Cientiacutefica de la Universidad de Granada (CIC UGR)
La distribucioacuten del tamantildeo hidrodinaacutemico de las NP se evaluoacute mediante anaacutelisis de
seguimiento de nanopartiacuteculas (NTA) con un NanoSight LM10-HS (GB) FT14 (NanoSight
Amesbury Reino Unido) y una caacutemara sCMOS La concentracioacuten de partiacuteculas de acuerdo con
el diaacutemetro (distribucioacuten de tamantildeos) se calculoacute como un promedio de al menos tres
distribuciones de tamantildeos independientes La concentracioacuten total de NP de cada sistema se
determinoacute para controlar el nuacutemero de partiacuteculas utilizadas en los experimentos celulares Las
condiciones de medicioacuten para todas las muestras fueron 25 deg C una viscosidad de 089 cP un
tiempo de medicioacuten de 60 s y una ganancia de caacutemara de 250 El obturador de la caacutemara fue de
11 y 15 ms para las NP vaciacuteas y cargadas con BMP respectivamente El umbral de deteccioacuten se
fijoacute en 5
La movilidad electroforeacutetica de las NP se determinoacute utilizando un dispositivo Zetasizerreg
NanoZeta ZS (Malvern Instrument Ltd Malvern Reino Unido) que funciona a 25 deg C con un
laacuteser He-Ne de 633 nm y un aacutengulo de dispersioacuten 173 Cada punto de datos se tomoacute como un
promedio sobre tres mediciones de muestra independientes Para cada muestra la distribucioacuten de
movilidad electroforeacutetica y la movilidad electroforeacutetica promedio (μ-promedio) se determinaron
mediante la teacutecnica de electroforesis Doppler laacuteser
523 Estabilidad coloidal y temporal en medios bioloacutegicos
El diaacutemetro hidrodinaacutemico promedio y el iacutendice de polidispersidad (PDI) por dispersioacuten
dinaacutemica de la luz (DLS) de cada sistema de NP se midieron en diferentes medios (tampoacuten de
fosfato (PB) tampoacuten de fosfato salino (PBS) y medio de cultivo celular Medio de Eagle
modificado de Dulbecco DMEM (Sigma)) Ademaacutes los datos sobre la estabilidad temporal se
102
obtuvieron repitiendo estos anaacutelisis en diferentes momentos despueacutes de la siacutentesis (0 1 y 5 diacuteas)
y despueacutes de 1 mes en condiciones de almacenamiento
Los experimentos de liberacioacuten in vitro se realizaron de la siguiente manera 1 ml de cada
muestra para cada tiempo de incubacioacuten se suspendioacute en PBS a 37 deg C Despueacutes del tiempo
correspondiente (24 48 96 168 h) las NP se separaron del sobrenadante de las proteiacutenas
liberadas por centrifugacioacuten durante 10 min a 14000 rpm (10 C) El sedimento de NP se suspendioacute
en 1 ml de NaOH 005 M y se agitoacute durante 2 h para una degradacioacuten completa del poliacutemero La
solucioacuten de proteiacutena alcalina se analizoacute mediante BCA y ELISA para cuantificar la cantidad
ineacutedita La proteiacutena liberada se calculoacute teniendo en cuenta la cantidad encapsulada total Todos
los experimentos se realizaron por triplicado
524 Interacciones celulares
Para todos los estudios bioloacutegicos in vitro se utilizoacute una poblacioacuten celular cultivada del hueso
alveolar maxilar Esta poblacioacuten se caracterizoacute previamente y se confirmoacute que presentaba todas
las caracteriacutesticas de una poblacioacuten de ceacutelulas del estroma mesenquimatoso (MSC) (Padial-
Molina et al 2019) Las ceacutelulas se tomaron de donantes humanos sanos despueacutes de la aprobacioacuten
del Comiteacute de Eacutetica para la Investigacioacuten Humana de la Universidad de Granada (424 CEIH
2018) Medio de Eagle modificado por Dulbecco regular (DMEM) con 1 g L de glucosa
(DMEM-LG) (Gibco) suero bovino fetal al 10 (FBS) (Sigma-Aldrich St Louis MO EE UU)
1 100 de aminoaacutecidos no esenciales (NEAA) (Gibco) 001 μg ml de factor de crecimiento de
fibroblastos baacutesico (bFGF) (PeproTech Londres Reino Unido) 100 U ml de penicilina
estreptomicina y 025 μg ml de anfotericina B utilizado como medio de cultivo para todos los
experimentos Los cultivos se mantuvieron a 37 deg C en una atmoacutesfera de CO2 al 5 (2000 ceacutelulas
pocillo) Todos los experimentos bioloacutegicos se repitieron por triplicado al menos 3 veces por
condicioacuten
19Migracioacuten Celular
103
Un ensayo de migracioacuten celular se realizoacute como se describioacute anteriormente (Padial-Molina
Volk and Rios 2014) (Liang Park and Guan 2007) Brevemente las MSC se distribuyeron en
tres pocillos para cada condicioacuten y se les permitioacute crecer hasta una confluencia celular cercana al
99 en 24 pocillos placa de 3000 ceacutelulas cm2 y en cada pocillo se realizaron tres rasguntildeos
diferentes Luego las ceacutelulas se privaron de hambre durante 24 h mediante la adicioacuten de medio
de cultivo sin suero Se hizo un rasguntildeo usando una punta de pipeta a lo largo del diaacutemetro del
pozo Se realizoacute un paso de lavado con PBS para eliminar las ceacutelulas rayadas Se antildeadieron nuevos
medios de cultivo completos y se suplementaron seguacuten el grupo asignado (BMP-2 NP-BMP2 y
NP-BSA-BMP2 a 125 25 y 5 ng ml de BMP-2) Posteriormente se tomaron nueve imaacutegenes
de la misma aacuterea en cada condicioacuten hasta 48 h maacutes tarde En estas imaacutegenes el aacuterea raspada se
midioacute con el software ImageJ (Instituto Nacional de Salud Bethesda MD EUA
(httprsbwebnihgovij) La reduccioacuten en el aacuterea rayada con el tiempo se midioacute considerando el
aacuterea en el tiempo 0 como 100 abierta
20Proliferacioacuten celular
La proliferacioacuten se evaluoacute mediante un ensayo de sulforhodamina (SRB) (Houghton et al
2007) El ensayo se realizoacute sembrando las ceacutelulas a 1500 ceacutelulas cm2 en una placa de 96 pocillos
a una confluencia no superior al 50 Despueacutes de la unioacuten celular se agregaron los diferentes
suplementos (BMP-2 NP-BMP2 y NP-BSA-BMP2 a 125 25 y 5 ng ml de BMP-2) y las ceacutelulas
se mantuvieron en cultivo durante 7 diacuteas En cada punto de tiempo las ceacutelulas se lavaron con PBS
1X y se fijaron antildeadiendo aacutecido tricloroaceacutetico al 10 enfriado con hielo durante 20 minutos a
4ordmC Luego las ceacutelulas se lavaron 3 veces con dH2O y se secaron hasta que se recogieron todos
los puntos de tiempo Cada pocillo recibioacute 04 de SRB en 1 de aacutecido aceacutetico durante 20
minutos a temperatura ambiente con agitacioacuten suave La tincioacuten se terminoacute lavando cada pocillo
3 veces con aacutecido aceacutetico al 1 y secaacutendolo a temperatura ambiente durante 24 h El colorante se
recuperoacute de las ceacutelulas antildeadiendo Tris Base 10 mM a pH 105 y agitando suavemente durante 10
104
minutos La solucioacuten recuperada se distribuyoacute luego en una placa de 96 pocillos y se leyoacute la
absorbancia oacuteptica a 492 nm
bullDiferenciacioacuten osteogeacutenica
La diferenciacioacuten osteogeacutenica se evaluoacute mediante la adicioacuten de medios osteogeacutenicos al cultivo
celular en combinacioacuten con BMP-2 NP-BMP2 y NP-BSA-BMP2 libres a las dosis maacutes altas
utilizadas en experimentos anteriores Las ceacutelulas se sembraron a 3000 ceacutelulas cm2 y se
cultivaron para alcanzar una confluencia del 85 al 90 Esto fue seguido por la adicioacuten de
medios de induccioacuten que conteniacutean 10 mM de glicerofosfato (Fluka 50020) 01 μM de
dexametasona (Sigma-Aldrich D2915) y 005 mM de aacutecido L-ascoacuterbico (Sigma-Aldrich
A8960) Los cultivos celulares se mantuvieron durante 7 diacuteas para analizar la actividad temprana
En el diacutea 7 las ceacutelulas se recogieron en 1 ml de TRIzolreg Luego se extrajo el ARN y se convirtioacute
en ADNc Luego se evaluoacute la fosfatasa alcalina (ALP) y se calculoacute la expresioacuten con respecto a
la proteiacutena gliceraldehiacutedo-3-fosfato deshidrogenasa (GAPDH) por el meacutetodo 2 DDCt Estos
procedimientos se llevaron a cabo como describe en otra parte el siguiente autor (Padial-Molina
et al 2019) Las secuencias de cebador directo e inverso fueron
AGCTCATTTCCTGGTATGACAAC y TTACTCCTTGGAGGCCATGTG para GAPDH
TCCAGGGATAAAGCAGGTCTTG y CTTTCTCTTTCTCTGGCACTAAGG para ALP
bullEvaluacioacuten estadiacutestica
La migracioacuten y la proliferacioacuten celular se evaluaron mediante ANOVA seguido de la prueba
de comparaciones muacuteltiples de Tukey para el anaacutelisis por pares La comparacioacuten entre los niveles
de ALP a los 4 frente a los 7 diacuteas se analizoacute mediante un par de pruebas t de Student En todos los
casos se establecioacute un valor p inferior a 005 como significacioacuten estadiacutestica
105
53 Resultados y Discusioacuten
531Formulacioacuten de nanoparticulas
La evaporacioacuten de doble emulsioacuten-solvente ha sido descrita como un meacutetodo robusto y de uso
frecuente para producir NP de PLGA cargadas con biomoleacuteculas (Ding and Zhu 2018)
(McClements 2018) (Ortega-Oller et al 2015) (Iqbal et al 2015) Una formulacioacuten previamente
optimizada por nuestro grupo permitioacute la preservacioacuten de la actividad bioloacutegica de biomoleacuteculas
encapsuladas usando un solvente orgaacutenico ligeramente agresivo Ademaacutes el aacutecido desoxicoacutelico
se ha utilizado en el primer paso de la formulacioacuten para mejorar la estabilidad coloidal de las NP
y simultaacuteneamente para obtener superficies de NP enriquecidas con grupos carboxiacutelicos
mejorando su versatilidad y permitiendo que un quiacutemico posterior de lugar a la inmovilizacioacuten de
diferentes ligandos especiacuteficos (Sanchez-Moreno et al 2013) Por medio de esta formulacioacuten
mejorada en el presente trabajo desarrollamos nanopartiacuteculas vaciacuteas (NP) o nanopartiacuteculas que
encapsulan rhBMP-2 (NP-BMP2)
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Una descripcioacuten esquemaacutetica del procedimiento de siacutentesis se muestra en la Figura 21
Figura 21 Esquema de la formulacioacuten de NP-BMP2
Para NP-BMP2 logramos una eficiencia de encapsulacioacuten de proteiacutenas (EE) de 97 plusmn 2 Este
resultado es estable en la literatura en la que varios autores han reportado valores igualmente altos
que encapsulan esta proteiacutena dentro de nanopartiacuteculas y micropartiacuteculas de PLGA (Lochmann et
al 2010) (Kempen et al 2008) Nuestra formulacioacuten tiene varios factores que conducen a este
elevado valor de EE La baja relacioacuten proteiacutena poliacutemero en masa (Manuel J Santander-Ortega
Csaba et al 2010) la afinidad de rhBMP-2 a una interaccioacuten inespeciacutefica con superficies
hidrofoacutebicas (Lochmann et al 2010) o la adicioacuten de estabilizadores (poloxaacutemero) en el segundo
paso del procedimiento de doble emulsioacuten (Ortega-Oller et al 2015) La ausencia de rhBMP-2
en el sobrenadante resultante de la etapa de centrifugacioacuten en el proceso de limpieza se verificoacute
mediante ELISA y SDS-PAGE en el que se muestra una banda clara correspondiente a 14 kD de
cadenas polipeptiacutedicas rhBMP-2 para el carril A en la Figura 22 correspondiente a NP-BMP2
La masa de proteiacutena encapsulada alrededor de 2 microg es similar a la de diferentes micro y
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nanosistemas PLGA descritos en la literatura (Wang et al 2015) (Chung et al 2007) (La et al
2010) Teniendo en cuenta las condiciones de almacenamiento para nuestras muestras esto
corresponde a 500 ng mL lo que representa una cantidad suficiente de concentracioacuten para
aplicaciones praacutecticas ya que este factor de crecimiento muestra actividades bioloacutegicas in vitro en
dosis muy bajas (5ndash20 ng ml) (Ortega-Oller et al 2015)
Figura 22 Anaacutelisis de electroforesis en gel de SDS-poliacrilamida (SDS-PAGE) en
condiciones reductoras de Nanopartiacuteculas de PLGA soacutelidas (NP de PLGA) y fracciones liacutequidas
(sobrenadante) de diferentes sistemas de NP Carril P proteiacutenas estaacutendares carril A NP-BMP2
(proteiacutena morfogeneacutetica oacutesea) carril B sobrenadante de NP-BMP2 despueacutes de la siacutentesis y
encapsulacioacuten de rhBMP-2 carril C NP despueacutes de la adsorcioacuten fiacutesica de BSA rhBMP-2 carril
D sobrenadante despueacutes de la adsorcioacuten fiacutesica de BSA (albuacutemina de suero bovino) rhBMP-2
en el sistema NP
Por otro lado resultoacute un segundo nanosistema modificando la forma en que rhBMP-2 es
incorporado en el nanoportador Hay varios ejemplos de adsorcioacuten superficial de diferentes
factores de crecimiento en micro y nanopartiacuteculas (La et al 2010) (Fu et al 2012) (Rahman et
al 2014) y la inmovilizacioacuten de la superficie sobre la encapsulacioacuten recientemente se ha
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propuesto como una forma de modular la liberacioacuten posterior de biomoleacuteculas Este proceso que
depende de la lenta difusioacuten de las biomoleacuteculas a traveacutes de la matriz polimeacuterica estaacute en
consecuencia altamente influenciado por la interaccioacuten proteiacutena-poliacutemero (Pakulska et al 2016)
(Fu et al 2017) y degradacioacuten del poliacutemero (Mir Ahmed and Rehman 2017) (Ding and Zhu
2018) Por lo tanto este nuevo enfoque en el uso de NP de PLGA para el suministro de
biomoleacuteculas se exploroacute inmovilizando la proteiacutena rhBMP-2 en la superficie de las NP vaciacuteas
mediante una simple adsorcioacuten fiacutesica Se sabe que este proceso se rige por interacciones
electrostaacuteticas e hidrofoacutebicas entre las moleacuteculas de proteiacutenas y las superficies NP (Peula and de
las Nieves 1993)
Para esto los grupos cargados en la superficie la hidrofilia la carga neta de las moleacuteculas de
proteiacutena y las caracteriacutesticas del medio de adsorcioacuten son los paraacutemetros de referencia Por lo
tanto disentildeamos un experimento de co-adsorcioacuten en el que interactuacutea una mezcla de rhBMP-2 y
BSA (04 p p rhBMP-2 BSA) simultaacuteneamente con la superficie de NP de PLGA Las
albuacuteminas se usan habitualmente como proteiacutenas protectoras cuando los factores de crecimiento
se incorporan en las NPs de PLGA (Ortega-Oller et al 2015) (Zhang et al 2016) Ademaacutes una
distribucioacuten superficial de las moleacuteculas de BSA puede mejorar la estabilidad coloidal de las NP
a pH fisioloacutegico debido a su carga negativa neta bajo estas condiciones (Peula and de las Nieves
1994) La Figura 23 muestra un esquema del proceso de co-adsorcioacuten La eficiencia de adsorcioacuten
es superior al 95 y en el SDS-PAGE de la Figura 22 se pueden ver dos bandas caracteriacutesticas
de ambas proteiacutenas en el carril C correspondiente al nanosistema NP-BSA-BMP2
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Figura 23 Esquema del proceso de adsorcioacuten de proteiacutenas para NP-BSA-BMP2
Sin embargo el carril D correspondiente a la ejecucioacuten del sobrenadante desde la
centrifugacioacuten del nanosistema despueacutes de los procesos de adsorcioacuten muestra la ausencia de
cualquier proteiacutena Este resultado se explica completamente teniendo en cuenta el pH del medio
(pH 50) cerca del punto isoeleacutectrico de BSA donde la adsorcioacuten de esta proteiacutena en
nanopartiacuteculas cargadas negativamente presenta un maacuteximo (Peula and de las Nieves 1993)
(Peula Hidalgo-Alvarez and de las Nieves 1995) La inmovilizacioacuten de rhBMP-2 en la superficie
cargada negativamente de las NPs demuestra que estaacuten favorecidos electrostaacuteticamente debido a
la carga neta positiva de esta proteiacutena a pH aacutecido y neutro
532Caracterizacioacuten de nanopartiacuteculas
21Tamantildeo de nanopartiacuteculas
Micrografiacuteas SEM y STEM (Figura 24) muestran que las muestras consisten en partiacuteculas
esfeacutericas de diaacutemetros diferentes (entre 150 y 450 nm) un rango similar al encontrado en un
trabajo anterior en el que las NP se cargaron con lisozima siguiendo un protocolo de siacutentesis
similar (Ortega-Oller et al 2017) En ese trabajo la teacutecnica DLS no pudo proporcionar una
distribucioacuten de tamantildeo confiable Por lo tanto la teacutecnica NTA fue directamente utilizada para
determinar el tamantildeo hidrodinaacutemico de las NP cargadas con BMP2
Las distribuciones de tamantildeo para NP vaciacuteas (NP) y cargadas con BMP (NP-BMP2) de NTA
(Figura 25 y videos S1 S2) fueron consistentes con las imaacutegenes SEM Se encontroacute que las
partiacuteculas con diaacutemetros entre 100 y 500 nm teniacutean la concentracioacuten de partiacuteculas maacutes alta en
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alrededor de 200 nm La carga con BMP tuvo un efecto en la distribucioacuten del tamantildeo lo que
condujo a picos maacutes definidos Estas mediciones nos permitieron determinar la concentracioacuten de
partiacuteculas en la muestra medida 688 plusmn 009 x 10 8 pp mL y 519 plusmn 012 x10 8 pp mL para
nanosistemas NP y NP-BMP2 respectivamente Estos valores fueron utilizados (teniendo en
cuenta la dilucioacuten correspondiente) para controlar el nuacutemero de partiacuteculas antildeadidas en los
experimentos celulares
Figura 24 Micrografiacutea de microscopiacutea electroacutenica de barrido (SEM) de nanopartiacuteculas
cargadas con rhBMP-2 (NP-BMP2)
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Figura 25 Distribucioacuten del diaacutemetro hidrodinaacutemico de NP (ciacuterculos) y NP-BMP2 (liacutenea
negra gruesa) medidos a pH 70 (tampoacuten de fosfato) por anaacutelisis de seguimiento de
nanopartiacuteculas (NTA)
bullMovilidad electrocineacutetica y estabilidad coloidal
La carga superficial de las nanopartiacuteculas se puede analizar mediante un estudio electrocineacutetico
midiendo la movilidad electroforeacutetica (microe) en diferentes condiciones La Figura 26 muestra la
movilidad electroforeacutetica y el potencial zeta evaluado para los tres nanosistemas NP NP- BMP2
y NP-BSA-BMP2 a baja fuerza ioacutenica y diferentes valores de pH La carga superficial eleacutectrica
de las NP reside en los grupos carboxiacutelicos de las no cubiertas por PLGA y moleacuteculas de aacutecido
desoxicoacutelico Estos grupos funcionalizados tambieacuten son uacutetiles debido a la posibilidad de una
vectorizacioacuten quiacutemica de la superficie para desarrollar nanoportadores de entrega dirigida
(Siafaka et al 2016)
Se confirmoacute previamente que la protonacioacuten de estos grupos de superficies aacutecidas a valores de
pH bajo su valor de pKa estaba estrechamente relacionado con una peacuterdida de carga superficial
y en consecuencia una reduccioacuten (en valor absoluto) de la movilidad electroforeacutetica del sistema
coloidal (Peula-Garciacutea Hidalgo-Alvarez and De Las Nieves 1997) (Manuel J Santander-Ortega
Lozano-Loacutepez et al 2010) Por lo general cuando las partiacuteculas coloidales estaacuten recubiertas por
Conce
ntr
acioacute
n d
e par
tiacutecu
las
(10
6 p
pm
L)
112
moleacuteculas de proteiacutena los valores de microe cambian notablemente en comparacioacuten con el mismo
valor de las superficies desnudas y estaacuten influenciadas por la carga eleacutectrica de las moleacuteculas de
proteiacutena adsorbidas (Peula-Garcia Hidaldo-Alvarez and De las Nieves 1997) (Santander-Ortega
Bastos-Gonzalez and Ortega-Vinuesa 2007) El comportamiento electrocineacutetico del sistema NP-
BMP2 sigue siendo similar al de NP y la encapsulacioacuten de rhBMP-2 no afecta la distribucioacuten de
carga superficial DAngelo y colaboradores informaron de un resultado similar al encapsular
diferentes factores de crecimiento en nanopartiacuteculas de mezcla de PLGA-poloxaacutemero en la
misma proporcioacuten p p de proteiacutena poliacutemero (drsquoAngelo et al 2010) Esto puede deberse a la
baja cantidad de proteiacutena encapsulada y su distribucioacuten en la parte interna de las NP (lejos de la
superficie) En nuestro sistema la distribucioacuten interna puede verse favorecida por las condiciones
de encapsulacioacuten donde el pH baacutesico (pH 120) del agua contiene rhBMP-2 que permite una carga
negativa de estas moleacuteculas de proteiacutena evitando asiacute su interaccioacuten electrostaacutetica especiacutefica con
grupos aacutecidos de las NP
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Figura 26 Movilidad electroforeacutetica y potencial zeta versus pH en medios de baja salinidad
(fuerza ioacutenica igual a 0002 M) para los diferentes nanosistemas (cuadrado negro) NP
(triaacutengulo azul) NP-BMP2 (ciacuterculo rojo) NP-BSA-BMP2
La distribucioacuten electrocineacutetica para el sistema NP-BSA-BMP2 cambia radicalmente Como se
mostroacute anteriormente la eficiencia de adsorcioacuten muy alta conduce a NP con ambas proteiacutenas
adsorbidas alrededor de su superficie Esta situacioacuten estaacute estrechamente relacionada con los
valores microe de la Figura 26 Teniendo en cuenta la relacioacuten p p entre proteiacutenas adsorbidas (250
veces mayor para BSA) las moleacuteculas de albuacutemina modulan el comportamiento a valores de pH
por debajo de su punto isoeleacutectrico (pI 47) donde la carga neta positiva BSA enmascara la carga
superficial original de las NP e incluso cambia sus valores originales a valores positivos Esto es
un resultado tiacutepico encontrado para estas partiacuteculas coloidales que cubren proteiacutenas (Peula
Hidalgo-Alvarez and de las Nieves 1995) (Peula JM Callejas J de las Nieves 1994) A
valores de pH neutros y baacutesicos las moleacuteculas de BSA tienen una carga neta negativa y la ligera
disminucioacuten en los valores absolutos microe podriacutea ser debido a la reduccioacuten de la carga superficial
neta negativa de los NP que pueden estar protegidos al menos en una pequentildea parte por la carga
positiva de las moleacuteculas de rhBMP-2 bajo su punto isoeleacutectrico baacutesico (pI 90)
Pote
nci
al z
eta
(mV
)
114
La estabilidad coloidal para los diferentes nanosistemas (NP NP-BMP2 y NP-BSA-BMP2)
fue determinada mediante el anaacutelisis de las distribuciones de tamantildeo en varios medios (PB PBS
y DMEM) en diferentes tiempos despueacutes de la siacutentesis (0 1 y 5 diacuteas) Se encontraron
distribuciones de tamantildeo similares a las originales para las dos formulaciones NP y NP-BMP2
en todos los medios analizados Este resultado fue similar al encontrado previamente para estos
tipos de NP que encapsulan la lisozima (Ortega-Oller et al 2017) en el que la combinacioacuten de
las interacciones electrostaacuteticas y esteacutericas generadas por grupos quiacutemicos superficiales de NP
confieren estabilidad al mecanismo que evita la agregacioacuten coloidal (Manuel J Santander-Ortega
Csaba et al 2010) La disminucioacuten del valor absoluto del potencial zeta para el sistema NP-
BSA-BMP2 como consecuencia de la distribucioacuten de proteiacutenas en la superficie no afecta su
estabilidad coloidal Este sistema tambieacuten mantiene la misma distribucioacuten de tamantildeos en los
diferentes medios Se acepta comuacutenmente que un potencial zeta superior a +30 o 30 mV daraacute
lugar a un sistema coloidal estable (Sun 2016) y el valor potencial zeta para NP-BSA-BMP2 es
superior a 30 mV La estabilidad coloidal en PBS y DMEM tiacutepicamente medios utilizados para
el desarrollo de interacciones celulares o scaffolds respectivamente asegura el uso potencial de
estos nanosistemas para entornos de vida in vitro o in vivo Ademaacutes estos sistemas mantuvieron
su tamantildeo bajo almacenamiento en PB a 4 deg C durante al menos 1 mes (datos no mostrados) lo
que demuestra que es un medio adecuado para el almacenamiento de muestras
bullLiberacioacuten de proteiacutenas
Uno de los principales problemas para los micro o nanosistemas de suministro de faacutermacos
PLGA es encontrar el patroacuten de liberacioacuten apropiado para las moleacuteculas de proteiacutenas encapsuladas
unidas Un amplio espectro de formulaciones modula esta propiedad mediante el uso de
diferentes tipos de procesos de siacutentesis poliacutemeros PLGA copoliacutemeros y estabilizadores (Mir
Ahmed and Rehman 2017) (Ortega-Oller et al 2015) Una limitacioacuten y control adecuados en la
liberacioacuten de estallido es fundamental para las BMP a fin de garantizar una liberacioacuten continua a
largo plazo que favorecida por la degradacioacuten del poliacutemero proporcione una mejor accioacuten in vivo
115
para impulsar la regeneracioacuten de hueso y cartiacutelago (Begam et al 2017) Por lo tanto previamente
desarrollamos un nanosistema PLGA dual para la liberacioacuten controlada a corto plazo donde la
difusioacuten de proteiacutenas y la interaccioacuten proteiacutena-poliacutemero son los principales factores que rigen este
proceso (Ortega-Oller et al 2017) En el presente trabajo los nanosistemas NP-BMP2 y NP-
BSA-BMP2 representan dos formas diferentes en las que se incorporoacute rhBMP-2 en el
nanoportador La Figura 27a muestra la liberacioacuten acumulativa de ambas proteiacutenas rhBMP-2 y
BSA para diferentes sistemas en funcioacuten del tiempo en un periacuteodo a corto plazo (7 diacuteas) La
proteiacutena rhBMP-2 encapsulada alcanza una cantidad liberada de alrededor del 30 de la proteiacutena
encapsulada inicial mientras que la rhBMP-2 adsorbida a pesar de su distribucioacuten superficial es
tres veces menor Sin embargo BSA muestra cantidades liberadas de hasta el 80 de los
adsorbidos iniciales En todos los casos las barras de error corresponden a las desviaciones
estaacutendar de tres experimentos independientes En estas condiciones el factor de crecimiento
encapsulado en NP-BMP2 presenta un patroacuten de liberacioacuten similar al encontrado previamente con
la misma formulacioacuten pero usando lisozima como proteiacutena (Ortega-Oller et al 2017) El
poloxaacutemero en la fase acuosa del proceso de siacutentesis puede ser clave para modular las
interacciones proteicas interfaciales especiacuteficas y no especiacuteficas (Del Castillo-Santaella et al
2019) Por lo tanto la relacioacuten entre la interaccioacuten proteiacutena-poliacutemero y la difusioacuten de proteiacutenas
parece estar bien equilibrada evitando un estallido inicial excesivo y al mismo tiempo
manteniendo el flujo de proteiacutenas necesario para liberar alrededor de un tercio de la rhBMP-2
encapsulada en 7 diacuteas Aunque se ha informado ampliamente de un estallido inicial excesivo para
los NP de PLGA relacionados con moleacuteculas de proteiacutenas cercanas a la superficie (Ding and Zhu
2018) esta situacioacuten no aparecioacute para el sistema NP-BMP2 siendo esto consistente con el
comportamiento electrocineacutetico que no mostroacute la presencia de proteiacutena cerca de la superficie La
literatura ofrece algunos ejemplos con liberacioacuten reducida a corto plazo de BMP-2 utilizando maacutes
copoliacutemeros PLGA-PEG hidroacutefilos (Kirby et al 2011) o un proceso de siacutentesis diferente (Chang
et al 2017)
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El rendimiento de la liberacioacuten del sistema NP-BSA-BMP2 que tambieacuten se muestra en la
Figura 27a presenta diferencias notables El perfil electrocineacutetico ha justificado previamente la
ubicacioacuten de la superficie de BSA y rhBMP-2 en la superficie lo que podriacutea conducir a una
liberacioacuten raacutepida de ambas proteiacutenas Sin embargo los resultados de la Figura 27a y 27b muestran
esta tendencia solo para la proteiacutena BSA que se libera de las NP con aproximadamente el 20
de la cantidad inicial restante despueacutes de siete diacuteas Sin embargo hasta el 90 de la carga inicial
de proteiacutena rhBMP-2 a diferencia de BSA permanece unida a la superficie La superficie NP con
grupos hidrofiacutelicos forma moleacuteculas de poloxaacutemero y una carga negativa debido a la abundante
presencia de grupos carboxiacutelicos (grupos terminales de PLGA y moleacuteculas de aacutecido desoxicoacutelico)
favorecen un proceso de desorcioacuten para BSA cuyas moleacuteculas tienen una carga negativa en
condiciones de liberacioacuten (pH fisioloacutegico) Esto concuerda con los resultados de otros autores
que incluso despueacutes de encapsular BSA en mezclas de PLGA-poloxaacutemero NP logroacute una
descarga de liberacioacuten raacutepida por encima del 40 o 50 de la cantidad inicial de proteiacutena (Manuel
J Santander-Ortega Csaba et al 2010) Ademaacutes la coencapsulacioacuten de albuacuteminas con factores
de crecimiento podriacutea afectar fuertemente su perfil de liberacioacuten causando un estallido inicial
(Balmayor et al 2009) (drsquoAngelo et al 2010) De lo contrario la atraccioacuten electrostaacutetica
especiacutefica entre las moleacuteculas rhBMP-2 positivas y los grupos de superficie negativos ralentiza la
liberacioacuten a corto plazo de esta proteiacutena Este resultado estaacute de acuerdo con la baja liberacioacuten de
BMP adsorbida encontrada previamente usando micro y nanopartiacuteculas de PLGA con grupos
terminales de aacutecido sin tapar (Pakulska et al 2016) (Schrier and DeLuca 2001) Por lo tanto la
combinacioacuten de diferentes meacutetodos para atrapar BMP-2 dentro y alrededor de NP muestra la
posibilidad de lograr una liberacioacuten controlada adecuadamente equilibrando las interacciones
entre poliacutemeros estabilizadores y proteiacutenas
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(a)
(b)
Figura 27 (a) Liberacioacuten acumulativa de rhBMP-2 para sistemas NP-BMP2 (cuadrado
negro) y NP-BSA-BMP2 (ciacuterculo rojo) y liberacioacuten acumulativa de BSA para el sistema NP-
BSA-BMP2 (triaacutengulo azul) incubado por diferentes tiempos a 37ordmC en tampoacuten de fosfato salino
(pH 74) (b) Anaacutelisis de SDS-PAGE en condiciones reductoras de fraccioacuten soacutelida de NP-BSA-
Lib
erac
ioacuten a
cum
ula
tiva
()
Tiempo (horas)
118
BMP2 despueacutes de la liberacioacuten en diferentes momentos en los que el nuacutemero de cada carril
corresponde al tiempo en horas
533Actividad bioloacutegica e interacciones
bullMigracioacuten Celular
La migracioacuten celular es el primer y necesario paso en la regeneracioacuten de tejidos (Padial-Molina
OrsquoValle et al 2015) Por lo tanto un agente regenerativo debe acelerar la migracioacuten celular o
al menos no interferir con ella En el presente estudio no encontramos diferencias entre los
grupos las dosis y el control en teacuterminos de cierre de un aacuterea rayada (ANOVA con la prueba de
comparaciones muacuteltiples de Tukey) (Figura 28) En contraste con nuestros hallazgos los datos
publicados anteriormente sugieren un efecto positivo de BMP-2 en la migracioacuten celular (Inai et
al 2008) (Gamell et al 2008) Sin embargo en esos estudios las dosis aplicadas y los tipos de
ceacutelulas fueron diferentes a los experimentos actuales Utilizamos dosis maacutes bajas de BMP-2 para
evaluar si incluso a dosis bajas BMP-2 podriacutea proporcionar beneficios si se protegiera en un
sistema de nanopartiacuteculas Como se mencionoacute no demostramos ninguacuten efecto negativo del
sistema en la migracioacuten celular Sin embargo nuestros resultados respaldan la idea de que la
actividad de BMP-2 estaacute mediada por la activacioacuten de la viacutea de la fosfoinositida 3-quinasa (PI3K)
un grupo comuacuten de moleacuteculas de sentildealizacioacuten que participan en varios procesos con BMP-2 y
otras moleacuteculas (Padial-Molina Volk and Rios 2014) (Gamell et al 2008) Tambieacuten debe
mencionarse que el plazo de un ensayo de migracioacuten es corto Por lo tanto las ventajas potenciales
de un sistema de liberacioacuten controlada como el que se estaacute estudiando podriacutean ser limitadas Es
decir la liberacioacuten de BMP-2 de las nanopartiacuteculas como se demuestra en la Figura 27 se limita
a las primeras 48 h Por lo tanto se podriacutea hipotetizar un efecto positivo sostenido sobre la
actividad migratoria a lo largo del tiempo
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Figura 28 Ensayo de migracioacuten Porcentaje de cierre del aacuterea rayada a las 24 y 48 h en
diferentes grupos y dosis
bullProliferacioacuten celular
La proliferacioacuten es otra de las actividades celulares requeridas para la regeneracioacuten de tejidos
Sin embargo esta propiedad debe equilibrarse con la migracioacuten y la diferenciacioacuten y no aumentar
las tres caracteriacutesticas al mismo tiempo y con las mismas proporciones (Friedrichs et al 2011)
De hecho seguacuten los informes cuando una dosis de BMP-2 induce una mayor proliferacioacuten
disminuye la diferenciacioacuten (Hrubi et al 2018) Esta propiedad ha sido ampliamente analizada
pero las discrepancias auacuten se pueden detectar en la literatura Por lo tanto Kim y colaboradores
analizoacute diferentes dosis de BMP-2 y su efecto sobre la proliferacioacuten celular y la apoptosis Se
confirmoacute in vitro que las dosis altas pero auacuten maacutes bajas que las utilizadas cliacutenicamente reducen
la proliferacioacuten celular y aumentan la apoptosis (Kim Oxendine and Kamiya 2013) Esto debe
ser evitado Hemos encontrado que aunque el BMP-2 libre no induce una proliferacioacuten mayor
que el control en ninguna de las dosis aplicadas ni en los puntos de tiempo (ANOVA con la prueba
0
10
20
30
40
50
60
70
80
90
100
0 h 24 h 48 h
o
f sc
ratc
hed
are
a
Time point
Control
BMP2-125 ngmL
BMP2-25 ngmL
BMP2-5 ngmL
NP-BMP2-125 ngmL
NP-BMP2-25 ngmL
NP-BMP2-5 ngmL
NP-BSA-BMP2-125 ngmL
NP-BSA-BMP2-25 ngmL
NP-BSA-BMP2-5 ngmL
d
e aacuter
ea r
ayad
a
Tiempo
120
de comparaciones muacuteltiples de Tukey) la misma cantidad de BMP-2 encapsulada o adsorbida en
nanopartiacuteculas de PLGA aumenta la proliferacioacuten siendo esto estadiacutesticamente significativo
cuando se usa una dosis de 25 ng mL o maacutes (ANOVA con prueba de comparaciones muacuteltiples
de Tukey) (Figura 29) Estas dosis son auacuten maacutes bajas que las sugeridas en estudios anteriores
Aparte de esa diferencia todaviacutea se logroacute un efecto positivo sobre la proliferacioacuten Ademaacutes
siguiendo el patroacuten de lanzamiento de la Figura 27 se espera que se libere maacutes BMP-2 con el
tiempo maacutes allaacute del marco de tiempo de 7 diacuteas Por lo tanto tambieacuten podriacutea esperarse un efecto
de induccioacuten sostenido hasta la confluencia completa del cultivo celular
Figura 29 Proliferacioacuten de ceacutelulas del estroma mesenquimatoso humano (MSC) medida por
absorbancia de sulforamida (SRB) Los resultados se normalizaron a T0 en cada grupo
T0 T1 T2 T3 T4 T6 T70
2
4
6
Time point
No
rmalized
Ab
so
rban
ce
NP-BSA-BMP2-125 ngmL
NP-BSA-BMP2-25 ngmL
NP-BSA-BMP2-5 ngmL
NP-BMP2-125 ngmL
NP-BMP2-25 ngmL
NP-BMP2-5 ngmL
BMP2-125 ngmL
BMP2-25 ngmL
BMP2-5 ngmL
Control
Abso
rban
cia
norm
aliz
ada
Tiempo
121
bullDiferenciacioacuten osteogeacutenica
Se ha confirmado que la diferenciacioacuten celular inducida por BMP-2 necesita la presencia de
componentes osteoinductores permisivos En particular se ha demostrado que el beta-
glicerofosfato ejerce un efecto sineacutergico con BMP-2 para inducir la diferenciacioacuten celular (Hrubi
et al 2018) Por lo tanto para probar la diferenciacioacuten osteogeacutenica analizamos la expresioacuten del
ARNm de ALP Se encontroacute que la actividad maacutexima de ALP se produce 10 diacuteas despueacutes de la
estimulacioacuten con micropartiacuteculas basadas en PLGA que contienen BMP-2 en coencapsulacioacuten
con albuacutemina seacuterica humana (Kirby et al 2011) Aunque otras pruebas podriacutean haberse utilizado
para reforzar nuestros hallazgos se sabe que ALP modula la deposicioacuten de noacutedulos mineralizados
lo que indica actividad osteoblaacutestica Para todo esto complementamos los medios de
diferenciacioacuten con Beta-glicerofosfato y BMP-2 NP-BMP2 o NP-BSA-BMP2 libres durante 4 y
7 diacuteas para poder capturar la dinaacutemica temprana de la expresioacuten del gen En nuestro estudio
identificamos un aumento en la expresioacuten de ALP en todos los grupos desde el diacutea 4 hasta el diacutea
7 (Figura 30) Aunque ALP en el diacutea 7 en el grupo BMP-2 parece ser maacutes alto que en los otros
dos grupos el cambio no resultoacute significativo De hecho las diferencias entre los grupos no fueron
estadiacutesticamente significativas en ninguacuten periacuteodo de tiempo Sin embargo cabe destacar que el
aumento no fue significativo dentro del grupo BMP-2 (p = 0141 prueba t de Student) pero fue
significativo dentro de los otros dos grupos (p = 0025 y p = 0003 NP-BMP2 y NP-BSA- Grupos
BMP2 respectivamente) Esto nuevamente podriacutea tomarse como una confirmacioacuten de la
liberacioacuten sostenida de la proteiacutena del sistema de nanopartiacuteculas maacutes allaacute de los puntos temporales
anteriores
Esto y los estudios de migracioacuten y proliferacioacuten descritos a continuacioacuten nos llevan a confirmar
que el sistema propuesto puede mantener una liberacioacuten adecuada de BMP-2 a lo largo del tiempo
manteniendo un efecto positivo en la migracioacuten y proliferacioacuten celular con dosis iniciales
reducidas de BMP-2 El hecho de que se evite el estallido inicial excesivo es importante para la
aplicacioacuten de esta nanotecnologiacutea en la regeneracioacuten oacutesea como en la odontologiacutea De esta
122
manera los efectos negativos de las altas dosis iniciales de BMP-2 se evitan al mismo tiempo que
la moleacutecula estaacute protegida contra la desnaturalizacioacuten dentro del NP Por lo tanto los efectos del
regenerador se mantienen con el tiempo Los experimentos in vitro mostraron que las NP de
PLGA cargadas con BMP-2 son los nanoportadores con el mejor perfil de liberacioacuten a corto plazo
sin una explosioacuten inicial y con una liberacioacuten moderada y sostenida de proteiacutena activa antes del
inicio de la degradacioacuten del poliacutemero Por lo tanto la actividad bioloacutegica es positiva sin
interaccioacuten negativa con la migracioacuten o la proliferacioacuten sino maacutes bien la induccioacuten de la
diferenciacioacuten celular a traveacutes de la expresioacuten de ALP
Figura 30 Cambio de pliegue relativo en la expresioacuten de ARNm de ALP (grupo de control
BMP2 a los 4 diacuteas) = Importancia estadiacutestica de la comparacioacuten a lo largo del tiempo (p =
0025 y p = 0003 prueba t del estudiante grupos NP-BMP2 y NP-BSA-BMP2)
Los experimentos de liberacioacuten in vitro muestran un patroacuten adecuado de administracioacuten a corto
plazo que al mismo tiempo preserva la bioactividad de la biomoleacutecula encapsulada Ademaacutes la
distribucioacuten de tamantildeo de nanopartiacutecula encontrada para este nanosistema W-F68 permite la
Tiempo
Cam
bio
de
pli
egue
123
posibilidad de una administracioacuten de proteiacutena dual externa e intracelular como se ha demostrado
mediante experimentos celulares in vitro Esta nueva formulacioacuten se utilizaraacute en futuros estudios
para encapsular y administrar factores de crecimiento in vitro e in vivo con el fin de explotar el
potencial terapeacuteutico de este nanosistema
Se ha puesto de manifiesto la necesidad de optimizacioacuten de los meacutetodos y componentes para
equilibrar la estructura y morfologiacutea de las micropartiacuteculas nanopartiacuteculas de PLGA logrando
de esta forma una alta eficiencia de encapsulacioacuten de BMP-2 y buscando un objetivo principal el
control de la entrega la reduccioacuten de la descarga inicial para alcanzar un perfil de liberacioacuten de
la proteiacutena sostenido en el tiempo preservando la actividad bioloacutegica y dirigieacutendola a ceacutelulas
diana para minimizar la cantidad cliacutenica de proteiacutena necesaria permitiendo al mismo tiempo una
correcta regeneracioacuten del tejido oacuteseo
En consecuencia otro reto futuro es conseguir el direccionamiento especiacutefico de estas nano-
esferas de PLGA cargadas de agentes activos Este aspecto se puede desarrollar mediante el uso
de unos ligandos que reconozcan especiacuteficamente los tipos o liacuteneas celulares a la que queremos
dirigir la liberacioacuten de biomoleacuteculas encapsuladas El uso de nanopartiacuteculas con una unioacuten
covalente de diferentes ligandos da lugar a una teacutecnica con un alto potencial de administracioacuten
que permite a la ingenieriacutea tisular un gran avance en cuanto a la distribucioacuten y administracioacuten de
diferentes faacutermacos o biomoleacuteculas mejorando asiacute las funciones bioloacutegicas o regenerativas
celulares
Los anticuerpos especiacuteficos que reconocen los receptores de superficie celular pueden unirse
covalentemente a la superficie de nuestras nanopartiacuteculas de PLGA dando lugar a una ldquoinmuno-
nanopartiacuteculardquo Para que esta unioacuten se produzca sin ninguacuten inconveniente en muchas ocasiones
hay que hacer uso de agentes estabilizadores para proporcionar estabilidad coloidal a las
nanopartiacuteculas sin que lleguen a afectar al enlace establecido entre los anticuerpos especiacuteficos de
los receptores celulares y las nanopartiacuteculas que es donde nos encontramos hoy diacutea y donde
124
muchos investigadores siguen haciendo avances cada diacutea entorno a la mejora de estos enlaces y
de estas entregas celulares especificas a traveacutes de las ldquoinmuno-nanopartiacuteculasrdquo
125
6CONCLUSIONES
En respuesta al objetivo principal de este trabajo
Los nanosistemas de partiacuteculas polimeacutericas basados en PLGA son sistemas prometedores para la
administracioacuten espacial y temporalmente controlada de factores de crecimiento que promueven
el desarrollo celular la diferenciacioacuten y regeneracioacuten en ingenieriacutea oacutesea mediante su
incorporacioacuten junto a las ceacutelulas en estructuras soacutelidas o hidrogeles
En respuesta a los objetivos secundarios de este trabajo
bullHa sido posible optimizar la obtencioacuten de diferentes sistemas de NPs de PLGA mediante un
procedimiento de doble emulsioacuten con las propiedades superficiales adecuadas que proporcionan
estabilidad coloidal y grupos carboxilo superficiales para unir quiacutemicamente diferentes ligandos
especiacuteficos en respuesta al objetivo 1
bullSe ha optimizado una formulacioacuten W-F68 que basada en el procedimiento anterior permite
encapsular moleacuteculas hidrofiacutelicas como las proteiacutenas obteniendo un novedoso nanotransportador
de tamantildeo dual que preserva la actividad bioloacutegica de la proteiacutena modelo encapsulada (lisozima)
en respuesta al objetivo 2
bullEn respuesta al tercer objetivo el anaacutelisis de la interaccioacuten proteiacutena-surfactante muestra el
papel crucial del solvente orgaacutenico el surfactante la relacioacuten de volumen entre ambas fases y la
carga neta de la proteiacutena encapsulada sobre las caracteriacutesticas finales de las NPs transportadoras
y el patroacuten de liberacioacuten proteica
bullLas experiencias in vitro de suministro proteico muestran una difusioacuten bien equilibrada de la
proteiacutena evitando una descarga inicial excesiva manteniendo un flujo constante de liberacioacuten a
corto plazo y permitiendo un suministro dual extra e intracelular sin citotoxicidad apreciable en
respuesta a los objetivos 4 y 5
126
bullSe ha desarrollado un nanosistema transportador de BMP2 sobre la base de un sistema modelo
formulado previamente mediante un procedimiento de doble emulsioacuten que conduce a un sistema
de NPs con una distribucioacuten dual de tamantildeos y estabilidad coloidal y temporal adecuada para
aplicaciones bioloacutegicas en respuesta al objetivo 6
bullIn vitro el Sistema con BMP2 encapsulada presenta un patroacuten de liberacioacuten en el corto plazo
7 diacuteas que muestra un suministro moderado y sostenido de proteiacutena bioloacutegicamente activa en
respuesta al objetivo 7
bullIn vitro la actividad bioloacutegica a nivel celular muestra mediante el anaacutelisis de la expresioacuten de
ALP la capacidad de la BMP2 nanotransportada para inducir diferenciacioacuten celular sin incidencia
negativa en los procesos de migracioacuten y proliferacioacuten celular en respuesta al objetivo 8
127
7 CONFLICTO DE INTERESES
Los autores declaran no tener ninguacuten conflicto de intereses con ninguno de los productos
enumerados en el documento
8 RECURSOS ECONOacuteMICOS
Beca de investigacioacuten obtenida competitivamente y otorgada por la empresa de implantes
dentales ldquoMIS IBERICA SLrdquo
Financiacioacuten parcial otorgada 1- por la Consejeriacutea de Economiacutea Innovacioacuten Educacioacuten
Ciencia y Empleo de la Junta de Andaluciacutea (Espantildea) 2- los proyectos MAT2013-43922-R ndash
incluyendo soporte europeo FEDER - (MICINN Espantildea) 3- y los Grupos de Investigacioacuten
FQM-115 CTS-1028 CTS-138 y CTS- 583 (Junta de Andaluciacutea Espantildea)
128
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Carragee E J Hurwitz E L and Weiner B K (2011) lsquoA critical review of recombinant
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deliveryrsquo Biomaterials 30(8) pp 1627ndash1634 doi 101016jbiomaterials200812013
Chang H-C et al (2017) lsquoBone morphogenetic protein-2 loaded poly(DL-lactide-co-
glycolide) microspheres enhance osteogenic potential of gelatinhydroxyapatitebeta-
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Chen G Deng C and Li Y-P (2012) lsquoTGF-beta and BMP signaling in osteoblast
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Cheng J et al (2007) lsquoFormulation of functionalized PLGA-PEG nanoparticles for in vivo
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Chou L Y T Ming K and Chan W C W (2011) lsquoStrategies for the intracellular delivery of
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Chung V H-Y et al (2012) lsquoEngineered autologous bone marrow mesenchymal stem cells
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Chung Y-I et al (2007) lsquoEnhanced bone regeneration with BMP-2 loaded functional
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biotechnology 10 pp 1ndash43 doi 1010070-306-46803-4_1
Csaba N et al (2004) lsquoDesign and characterisation of new nanoparticulate polymer blends
for drug deliveryrsquo Journal of biomaterials science Polymer edition 15(9) pp 1137ndash1151
doi 1011631568562041753098
Csaba N et al (2005) lsquoPLGApoloxamer and PLGApoloxamine blend nanoparticles new
carriers for gene deliveryrsquo Biomacromolecules 6(1) pp 271ndash278 doi
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Csaba N Garcia-Fuentes M and Alonso M J (2006) lsquoThe performance of nanocarriers for
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Danhier F et al (2012) lsquoPLGA-based nanoparticles an overview of biomedical
applicationsrsquo Journal of controlled release official journal of the Controlled Release Society
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101016jmolmed200907002
Devine J G et al (2012) lsquoThe use of rhBMP in spine surgery is there a cancer riskrsquo
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of wheat germ agglutinin-functionalized Poly(DL-lactic-co-glycolic acid)-microspheresrsquo
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Fang D-L et al (2014) lsquoDevelopment of lipid-shell and polymer core nanoparticles with
water-soluble salidroside for anti-cancer therapyrsquo International journal of molecular
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Farace C et al (2016) lsquoImmune cell impact of three differently coated lipid nanocapsules
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and microparticles by PVA poloxamer and PVPrsquo Colloids and Surfaces A Physicochemical
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Feng S and Huang G (2001) lsquoEffects of emulsifiers on the controlled release of paclitaxel
(Taxol) from nanospheres of biodegradable polymersrsquo Journal of controlled release official
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3659(00)00364-3
Fraylich M et al (2008) lsquoPoly(DL-lactide-co-glycolide) dispersions containing pluronics
from particle preparation to temperature-triggered aggregationrsquo Langmuir the ACS
journal of surfaces and colloids 24(15) pp 7761ndash7768 doi 101021la800869u
Fredenberg S et al (2011) lsquoThe mechanisms of drug release in poly(lactic-co-glycolic acid)-
based drug delivery systems--a reviewrsquo International journal of pharmaceutics 415(1ndash2)
pp 34ndash52 doi 101016jijpharm201105049
Friedrichs M et al (2011) lsquoBMP signaling balances proliferation and differentiation of
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26
Froum S J et al (2006) lsquoComparison of mineralized cancellous bone allograft (Puros) and
anorganic bovine bone matrix (Bio-Oss) for sinus augmentation histomorphometry at 26
to 32 weeks after graftingrsquo The International journal of periodontics amp restorative dentistry
26(6) pp 543ndash551
Fu C et al (2017) lsquoEnhancing Cell Proliferation and Osteogenic Differentiation of MC3T3-
E1 Pre-osteoblasts by BMP-2 Delivery in Graphene Oxide-Incorporated PLGAHA
Biodegradable Microcarriersrsquo Scientific reports 7(1) p 12549 doi 101038s41598-017-
12935-x
Fu R et al (2013) lsquoEffectiveness and harms of recombinant human bone morphogenetic
protein-2 in spine fusion a systematic review and meta-analysisrsquo Annals of internal
medicine 158(12) pp 890ndash902 doi 1073260003-4819-158-12-201306180-00006
Fu Y et al (2012) lsquoIn vitro sustained release of recombinant human bone morphogenetic
protein-2 microspheres embedded in thermosensitive hydrogelsrsquo Die Pharmazie 67(4) pp
299ndash303
Galindo-Moreno P et al (2007) lsquoEvaluation of sinus floor elevation using a composite bone
graft mixturersquo Clinical oral implants research 18(3) pp 376ndash382 doi 101111j1600-
0501200701337x
Galindo-Moreno P et al (2011) lsquoEffect of anorganic bovine bone to autogenous cortical
bone ratio upon bone remodeling patterns following maxillary sinus augmentationrsquo Clinical
oral implants research 22(8) pp 857ndash864 doi 101111j1600-0501201002073x
Gamell C et al (2008) lsquoBMP2 induction of actin cytoskeleton reorganization and cell
migration requires PI3-kinase and Cdc42 activityrsquo Journal of cell science 121(Pt 23) pp
3960ndash3970 doi 101242jcs031286
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Gaudana R et al (2013) lsquoDesign and evaluation of a novel nanoparticulate-based
formulation encapsulating a HIP complex of lysozymersquo Pharmaceutical development and
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Ghaderi R and Carlfors J (1997) lsquoBiological activity of lysozyme after entrapment in
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1562 doi 101023a1012122200381
Giteau A et al (2008) lsquoHow to achieve sustained and complete protein release from PLGA-
based microparticlesrsquo International journal of pharmaceutics 350(1ndash2) pp 14ndash26 doi
101016jijpharm200711012
Gref R et al (1994) lsquoBiodegradable long-circulating polymeric nanospheresrsquo Science (New
York NY) 263(5153) pp 1600ndash1603 doi 101126science8128245
Hans M L and Lowman A M (2002) lsquoBiodegradable nanoparticles for drug delivery and
targetingrsquo Current Opinion in Solid State and Materials Science 6(4) pp 319ndash327 doi
101016S1359-0286(02)00117-1
Hassan P A Rana S and Verma G (2015) lsquoMaking sense of Brownian motion colloid
characterization by dynamic light scatteringrsquo Langmuir the ACS journal of surfaces and
colloids 31(1) pp 3ndash12 doi 101021la501789z
Hines D J and Kaplan D L (2013) lsquoPoly(lactic-co-glycolic) acid-controlled-release systems
experimental and modeling insightsrsquo Critical reviews in therapeutic drug carrier systems
30(3) pp 257ndash276 doi 101615critrevtherdrugcarriersyst2013006475
Hong P et al (2013) lsquoEnhancement of bone consolidation in mandibular distraction
osteogenesis a contemporary review of experimental studies involving adjuvant
therapiesrsquo Journal of plastic reconstructive amp aesthetic surgery JPRAS 66(7) pp 883ndash895
doi 101016jbjps201303030
Houghton P et al (2007) lsquoThe sulphorhodamine (SRB) assay and other approaches to
136
testing plant extracts and derived compounds for activities related to reputed anticancer
activityrsquo Methods (San Diego Calif) 42(4) pp 377ndash387 doi 101016jymeth200701003
Hrubi E et al (2018) lsquoDiverse effect of BMP-2 homodimer on mesenchymal progenitors of
different originrsquo Human cell 31(2) pp 139ndash148 doi 101007s13577-018-0202-5
Inai K et al (2008) lsquoBMP-2 induces cell migration and periostin expression during
atrioventricular valvulogenesisrsquo Developmental biology 315(2) pp 383ndash396 doi
101016jydbio200712028
Iqbal M et al (2015) lsquoDouble emulsion solvent evaporation techniques used for drug
encapsulationrsquo International journal of pharmaceutics 496(2) pp 173ndash190 doi
101016jijpharm201510057
Jana Sougata and Jana Subrata (2017) lsquoNatural polymeric biodegradable nanoblend for
macromolecules deliveryrsquo in Recent Developments in Polymer Macro Micro and Nano Blends
Preparation and Characterisation Elsevier Inc pp 289ndash312 doi 101016B978-0-08-
100408-100010-8
Jeon O et al (2008) lsquoLong-term delivery enhances in vivo osteogenic efficacy of bone
morphogenetic protein-2 compared to short-term deliveryrsquo Biochemical and biophysical
research communications 369(2) pp 774ndash780 doi 101016jbbrc200802099
Ji W et al (2012) lsquoLocal delivery of small and large biomolecules in craniomaxillofacial
bonersquo Advanced drug delivery reviews 64(12) pp 1152ndash1164 doi
101016jaddr201203003
Ji Y et al (2010) lsquoBMP-2PLGA delayed-release microspheres composite graft selection of
bone particulate diameters and prevention of aseptic inflammation for bone tissue
engineeringrsquo Annals of biomedical engineering 38(3) pp 632ndash639 doi 101007s10439-
009-9888-6
Jiang W et al (2005) lsquoBiodegradable poly(lactic-co-glycolic acid) microparticles for
137
injectable delivery of vaccine antigensrsquo Advanced drug delivery reviews 57(3) pp 391ndash410
doi 101016jaddr200409003
Kao D W K et al (2012) lsquoThe negative effect of combining rhBMP-2 and Bio-Oss on bone
formation for maxillary sinus augmentationrsquo The International journal of periodontics amp
restorative dentistry 32(1) pp 61ndash67
Katranji A Fotek P and Wang H-L (2008) lsquoSinus augmentation complications etiology
and treatmentrsquo Implant dentistry 17(3) pp 339ndash349 doi
101097ID0b013e3181815660
Kempen D H R et al (2008) lsquoRetention of in vitro and in vivo BMP-2 bioactivities in
sustained delivery vehicles for bone tissue engineeringrsquo Biomaterials 29(22) pp 3245ndash
3252 doi 101016jbiomaterials200804031
Kempen D H R et al (2009) lsquoEffect of local sequential VEGF and BMP-2 delivery on ectopic
and orthotopic bone regenerationrsquo Biomaterials 30(14) pp 2816ndash2825 doi
101016jbiomaterials200901031
Ki-Bum Lee Ani Solanki J Dongun Kim J J (2009) Nanomedicine Dynamic Integration of
Nanotechnology with Biomedical Science | Request PDF Available at
httpswwwresearchgatenetpublication254745458_Nanomedicine_Dynamic_Integrati
on_of_Nanotechnology_with_Biomedical_Science (Accessed 29 March 2020)
Kim H K W Oxendine I and Kamiya N (2013) lsquoHigh-concentration of BMP2 reduces cell
proliferation and increases apoptosis via DKK1 and SOST in human primary periosteal
cellsrsquo Bone 54(1) pp 141ndash150 doi 101016jbone201301031
Kim Y-H and Tabata Y (2015) lsquoDual-controlled release system of drugs for bone
regenerationrsquo Advanced drug delivery reviews 94 pp 28ndash40 doi
101016jaddr201506003
Kirby G T S et al (2011) lsquoPLGA-based microparticles for the sustained release of BMP-2rsquo
138
in European Cells and Materials AO Research Institute Davos p 24 doi
103390polym3010571
Kocbek P et al (2007) lsquoTargeting cancer cells using PLGA nanoparticles surface modified
with monoclonal antibodyrsquo Journal of controlled release official journal of the Controlled
Release Society 120(1ndash2) pp 18ndash26 doi 101016jjconrel200703012
Kok R J et al (1998) lsquoDrug delivery to the kidneys and the bladder with the low molecular
weight protein lysozymersquo Renal failure 20(2) pp 211ndash217 doi
10310908860229809045104
Kumar B et al (2017) lsquoRecent advances in nanoparticle-mediated drug deliveryrsquo Journal of
Drug Delivery Science and Technology Editions de Sante pp 260ndash268 doi
101016jjddst201707019
Kumar T R S Soppimath K and Nachaegari S K (2006) lsquoNovel delivery technologies for
protein and peptide therapeuticsrsquo Current pharmaceutical biotechnology 7(4) pp 261ndash
276 doi 102174138920106777950852
Kumari A Yadav S K and Yadav S C (2010) lsquoBiodegradable polymeric nanoparticles
based drug delivery systemsrsquo Colloids and surfaces B Biointerfaces 75(1) pp 1ndash18 doi
101016jcolsurfb200909001
La W-G et al (2010) lsquoThe efficacy of bone morphogenetic protein-2 depends on its mode
of deliveryrsquo Artificial organs 34(12) pp 1150ndash1153 doi 101111j1525-
1594200900988x
Lee J et al (2013) lsquoSinus augmentation using rhBMP-2ACS in a mini-pig model relative
efficacy of autogenous fresh particulate iliac bone graftsrsquo Clinical oral implants research
24(5) pp 497ndash504 doi 101111j1600-0501201102419x
Lee S-J et al (2017) lsquoDevelopment of Novel 3-D Printed Scaffolds With Core-Shell
Nanoparticles for Nerve Regenerationrsquo IEEE transactions on bio-medical engineering 64(2)
139
pp 408ndash418 doi 101109TBME20162558493
Li B et al (2009) lsquoThe effects of rhBMP-2 released from biodegradable
polyurethanemicrosphere composite scaffolds on new bone formation in rat femorarsquo
Biomaterials 30(35) pp 6768ndash6779 doi 101016jbiomaterials200908038
Liang C-C Park A Y and Guan J-L (2007) lsquoIn vitro scratch assay a convenient and
inexpensive method for analysis of cell migration in vitrorsquo Nature protocols 2(2) pp 329ndash
333 doi 101038nprot200730
LIN Y et al (2007) lsquoIn vitro Evaluation of Lysozyme-loaded Microspheres in
Thermosensitive Methylcellulose-based Hydrogel1 1 Supported by the National Natural
Science Foundation of China (No20576057) and Fundamental Research Foundation of
Tsinghua University (JCqn2005033)rsquo Chinese Journal of Chemical Engineering 15(4) pp
566ndash572 doi 101016S1004-9541(07)60125-6
Lochmann A et al (2010) lsquoThe influence of covalently linked and free polyethylene glycol
on the structural and release properties of rhBMP-2 loaded microspheresrsquo Journal of
controlled release official journal of the Controlled Release Society 147(1) pp 92ndash100 doi
101016jjconrel201006021
Loureiro J A et al (2016) lsquoCellular uptake of PLGA nanoparticles targeted with anti-amyloid
and anti-transferrin receptor antibodies for Alzheimerrsquos disease treatmentrsquo Colloids and
surfaces B Biointerfaces 145 pp 8ndash13 doi 101016jcolsurfb201604041
Luginbuehl V et al (2004) lsquoLocalized delivery of growth factors for bone repairrsquo European
journal of pharmaceutics and biopharmaceutics official journal of Arbeitsgemeinschaft fur
Pharmazeutische Verfahrenstechnik eV 58(2) pp 197ndash208 doi
101016jejpb200403004
M Padial-Molina P Galindo-Moreno G Aacute-M (2009) lsquoBiomimetic ceramics in implant
dentistryrsquo MINERVA BIOTECNOLOGICA 21 p 173
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Makadia H K and Siegel S J (2011) lsquoPoly Lactic-co-Glycolic Acid (PLGA) as Biodegradable
Controlled Drug Delivery Carrierrsquo Polymers 3(3) pp 1377ndash1397 doi
103390polym3031377
Maldonado-Valderrama J et al (2013) lsquoIn vitro digestion of interfacial protein structuresrsquo
Soft Matter 9(4) pp 1043ndash1053 doi 101039c2sm26843d
Mason S et al (2014) lsquoStandardization and safety of alveolar bone-derived stem cell
isolationrsquo Journal of dental research 93(1) pp 55ndash61 doi 1011770022034513510530
McClements D J (2018) lsquoEncapsulation protection and delivery of bioactive proteins and
peptides using nanoparticle and microparticle systems A reviewrsquo Advances in colloid and
interface science 253 pp 1ndash22 doi 101016jcis201802002
McKay W F Peckham S M and Badura J M (2007) lsquoA comprehensive clinical review of
recombinant human bone morphogenetic protein-2 (INFUSE Bone Graft)rsquo International
orthopaedics 31(6) pp 729ndash734 doi 101007s00264-007-0418-6
Meinel L et al (2001) lsquoStabilizing insulin-like growth factor-I in poly(DL-lactide-co-
glycolide) microspheresrsquo Journal of controlled release official journal of the Controlled
Release Society 70(1ndash2) pp 193ndash202 doi 101016s0168-3659(00)00352-7
Meng F T et al (2003) lsquoWOW double emulsion technique using ethyl acetate as organic
solvent effects of its diffusion rate on the characteristics of microparticlesrsquo Journal of
controlled release official journal of the Controlled Release Society 91(3) pp 407ndash416 doi
101016s0168-3659(03)00273-6
Mir M Ahmed N and Rehman A U (2017) lsquoRecent applications of PLGA based
nanostructures in drug deliveryrsquo Colloids and surfaces B Biointerfaces 159 pp 217ndash231
doi 101016jcolsurfb201707038
Misch C E (1987) lsquoMaxillary sinus augmentation for endosteal implants organized
alternative treatment plansrsquo The International journal of oral implantology implantologist
141
4(2) pp 49ndash58
Mohamed F and van der Walle C F (2008) lsquoEngineering biodegradable polyester particles
with specific drug targeting and drug release propertiesrsquo Journal of pharmaceutical sciences
97(1) pp 71ndash87 doi 101002jps21082
Morille M et al (2013) lsquoNew PLGA-P188-PLGA matrix enhances TGF-beta3 release from
pharmacologically active microcarriers and promotes chondrogenesis of mesenchymal
stem cellsrsquo Journal of controlled release official journal of the Controlled Release Society
170(1) pp 99ndash110 doi 101016jjconrel201304017
Mueller T D and Nickel J (2012) lsquoPromiscuity and specificity in BMP receptor activationrsquo
FEBS letters 586(14) pp 1846ndash1859 doi 101016jfebslet201202043
Myeroff C and Archdeacon M (2011) lsquoAutogenous bone graft donor sites and techniquesrsquo
The Journal of bone and joint surgery American volume 93(23) pp 2227ndash2236 doi
102106JBJSJ01513
Nair B P and Sharma C P (2012) lsquoPoly(lactide-co-glycolide)-laponite-F68 nanocomposite
vesicles through a single-step double-emulsion method for the controlled release of
doxorubicinrsquo Langmuir the ACS journal of surfaces and colloids 28(9) pp 4559ndash4564 doi
101021la300005c
Nevins M et al (1996) lsquoBone formation in the goat maxillary sinus induced by absorbable
collagen sponge implants impregnated with recombinant human bone morphogenetic
protein-2rsquo The International journal of periodontics amp restorative dentistry 16(1) pp 8ndash19
Oh S H Kim T H and Lee J H (2011) lsquoCreating growth factor gradients in three
dimensional porous matrix by centrifugation and surface immobilizationrsquo Biomaterials
32(32) pp 8254ndash8260 doi 101016jbiomaterials201107027
Ortega-Oller I et al (2015) lsquoBone Regeneration from PLGA Micro-Nanoparticlesrsquo BioMed
research international 2015 p 415289 doi 1011552015415289
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Ortega-Oller I et al (2017) lsquoDual delivery nanosystem for biomolecules Formulation
characterization and in vitro releasersquo Colloids and surfaces B Biointerfaces 159 pp 586ndash
595 doi 101016jcolsurfb201708027
Padial-Molina M et al (2012) lsquoMethods to validate tooth-supporting regenerative
therapiesrsquo Methods in molecular biology (Clifton NJ) 887 pp 135ndash148 doi 101007978-
1-61779-860-3_13
Padial-Molina M OrsquoValle F et al (2015) lsquoClinical Application of Mesenchymal Stem Cells
and Novel Supportive Therapies for Oral Bone Regenerationrsquo BioMed research
international 2015 p 341327 doi 1011552015341327
Padial-Molina M Rodriguez J C et al (2015) lsquoStandardized in vivo model for studying
novel regenerative approaches for multitissue bone-ligament interfacesrsquo Nature protocols
10(7) pp 1038ndash1049 doi 101038nprot2015063
Padial-Molina M et al (2019) lsquoExpression of Musashi-1 During Osteogenic Differentiation
of Oral MSC An In Vitro Studyrsquo International journal of molecular sciences 20(9) doi
103390ijms20092171
Padial-Molina M and Rios H F (2014) lsquoStem Cells Scaffolds and Gene Therapy for
Periodontal Engineeringrsquo Current Oral Health Reports 1(1) pp 16ndash25 doi
101007s40496-013-0002-7
Padial-Molina M Volk S L and Rios H F (2014) lsquoPeriostin increases migration and
proliferation of human periodontal ligament fibroblasts challenged by tumor necrosis factor
-alpha and Porphyromonas gingivalis lipopolysaccharidesrsquo Journal of periodontal research
49(3) pp 405ndash414 doi 101111jre12120
Paillard-Giteau A et al (2010) lsquoEffect of various additives and polymers on lysozyme
release from PLGA microspheres prepared by an sow emulsion techniquersquo European
journal of pharmaceutics and biopharmaceutics official journal of Arbeitsgemeinschaft fur
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Pharmazeutische Verfahrenstechnik eV 75(2) pp 128ndash136 doi
101016jejpb201003005
Pakulska M M et al (2016) lsquoEncapsulation-free controlled release Electrostatic adsorption
eliminates the need for protein encapsulation in PLGA nanoparticlesrsquo Science advances
2(5) p e1600519 doi 101126sciadv1600519
Pantazis P et al (2012) lsquoPreparation of siRNA-encapsulated PLGA nanoparticles for
sustained release of siRNA and evaluation of encapsulation efficiencyrsquo Methods in molecular
biology (Clifton NJ) 906 pp 311ndash319 doi 101007978-1-61779-953-2_25
Panyam J and Labhasetwar V (2003) lsquoDynamics of endocytosis and exocytosis of poly(DL-
lactide-co-glycolide) nanoparticles in vascular smooth muscle cellsrsquo Pharmaceutical
research 20(2) pp 212ndash220 doi 101023a1022219003551
Paolicelli P et al (2010) lsquoSurface-modified PLGA-based nanoparticles that can efficiently
associate and deliver virus-like particlesrsquo Nanomedicine (London England) 5(6) pp 843ndash
853 doi 102217nnm1069
Park J S et al (2013) lsquoMultilineage differentiation of human-derived dermal fibroblasts
transfected with genes coated on PLGA nanoparticles plus growth factorsrsquo Biomaterials
34(2) pp 582ndash597 doi 101016jbiomaterials201210001
Penaloza J P et al (2017) lsquoIntracellular trafficking and cellular uptake mechanism of PHBV
nanoparticles for targeted delivery in epithelial cell linesrsquo Journal of nanobiotechnology
15(1) p 1 doi 101186s12951-016-0241-6
Perez C De Jesus P and Griebenow K (2002) lsquoPreservation of lysozyme structure and
function upon encapsulation and release from poly(lactic-co-glycolic) acid microspheres
prepared by the water-in-oil-in-water methodrsquo International journal of pharmaceutics
248(1ndash2) pp 193ndash206 doi 101016s0378-5173(02)00435-0
Peula-Garcia J M Hidaldo-Alvarez R and De las Nieves F J (1997) lsquoProtein co-adsorption
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on different polystyrene latexes Electrokinetic characterization and colloidal stabilityrsquo
Colloid and Polymer Science 275(2) pp 198ndash202 doi 101007s003960050072
Peula-Garciacutea J M Hidalgo-Alvarez R and De Las Nieves F J (1997) lsquoColloid stability and
electrokinetic characterization of polymer colloids prepared by different methodsrsquo Colloids
and Surfaces A Physicochemical and Engineering Aspects 127(1ndash3) pp 19ndash24 doi
101016S0927-7757(96)03890-3
Peula JM Callejas J de las Nieves F J (1994) lsquoAdsorption of Monomeric Bovine Serum
Albumin on Sulfonated Polystyrene Model Colloids II Electrokinetic Characterization of
Latex-Protein Complexesrsquo Surface Properties of Biomaterials pp 61ndash69
Peula J M Hidalgo-Alvarez R and de las Nieves F J (1995) lsquoCoadsorption of IgG and BSA
onto sulfonated polystyrene latex I Sequential and competitive coadsorption isothermsrsquo
Journal of biomaterials science Polymer edition 7(3) pp 231ndash240 doi
101163156856295x00274
Peula J M and de las Nieves F J (1993) lsquoAdsorption of monomeric bovine serum albumin
on sulfonated polystyrene model colloids 1 Adsorption isotherms and effect of the surface
charge densityrsquo Colloids and Surfaces A Physicochemical and Engineering Aspects 77(3) pp
199ndash208 doi 1010160927-7757(93)80117-W
Peula J M and de las Nieves F J (1994) lsquoAdsorption of monomeric bovine serum albumin
on sulfonated polystyrene model colloids 3 Colloidal stability of latex-protein complexesrsquo
Colloids and Surfaces A Physicochemical and Engineering Aspects 90(1) pp 55ndash62 doi
1010160927-7757(94)02889-3
Pezennec S et al (2008) The protein net electric charge determines the surface rheological
properties of ovalbumin adsorbed at the air-water interface Available at
wwwelseviercomlocatefoodhyd (Accessed 29 March 2020)
Pirooznia N et al (2012) lsquoEncapsulation of alpha-1 antitrypsin in PLGA nanoparticles in
145
vitro characterization as an effective aerosol formulation in pulmonary diseasesrsquo Journal of
nanobiotechnology 10 p 20 doi 1011861477-3155-10-20
Poinern G E J (no date) A laboratory course in nanoscience and nanotechnology
Puppi D et al (2014) lsquoNanomicrofibrous polymeric constructs loaded with bioactive
agents and designed for tissue engineering applications a reviewrsquo Journal of biomedical
materials research Part B Applied biomaterials 102(7) pp 1562ndash1579 doi
101002jbmb33144
Qutachi O Shakesheff K M and Buttery L D K (2013) lsquoDelivery of definable number of
drug or growth factor loaded poly(DL-lactic acid-co-glycolic acid) microparticles within
human embryonic stem cell derived aggregatesrsquo Journal of controlled release official
journal of the Controlled Release Society 168(1) pp 18ndash27 doi
101016jjconrel201302029
Rafati A et al (2012) lsquoChemical and spatial analysis of protein loaded PLGA microspheres
for drug delivery applicationsrsquo Journal of controlled release official journal of the Controlled
Release Society 162(2) pp 321ndash329 doi 101016jjconrel201205008
Rahman C V et al (2014) lsquoControlled release of BMP-2 from a sintered polymer scaffold
enhances bone repair in a mouse calvarial defect modelrsquo Journal of tissue engineering and
regenerative medicine 8(1) pp 59ndash66 doi 101002term1497
Ramel M-C and Hill C S (2012) lsquoSpatial regulation of BMP activityrsquo FEBS letters 586(14)
pp 1929ndash1941 doi 101016jfebslet201202035
Ratzinger G et al (2010) lsquoSurface modification of PLGA particles the interplay between
stabilizer ligand size and hydrophobic interactionsrsquo Langmuir the ACS journal of surfaces
and colloids 26(3) pp 1855ndash1859 doi 101021la902602z
Rescignano N et al (2016) lsquoIn-vitro degradation of PLGA nanoparticles in aqueous medium
and in stem cell cultures by monitoring the cargo fluorescence spectrumrsquo Polymer
146
Degradation and Stability 134 pp 296ndash304 doi 101016jpolymdegradstab201610017
van Rijt S and Habibovic P (2017) lsquoEnhancing regenerative approaches with
nanoparticlesrsquo Journal of the Royal Society Interface 14(129) doi 101098rsif20170093
Romagnoli C DrsquoAsta F and Brandi M L (2013) lsquoDrug delivery using composite scaffolds
in the context of bone tissue engineeringrsquo Clinical cases in mineral and bone metabolism
the official journal of the Italian Society of Osteoporosis Mineral Metabolism and Skeletal
Diseases 10(3) pp 155ndash161
Ronga M et al (2013) lsquoClinical applications of growth factors in bone injuries experience
with BMPsrsquo Injury 44 Suppl 1 pp S34-9 doi 101016S0020-1383(13)70008-1
Rosca I D Watari F and Uo M (2004) lsquoMicroparticle formation and its mechanism in
single and double emulsion solvent evaporationrsquo Journal of controlled release official
journal of the Controlled Release Society 99(2) pp 271ndash280 doi
101016jjconrel200407007
Sanchez-Moreno P et al (2013) lsquoSynthesis and characterization of lipid immuno-
nanocapsules for directed drug delivery selective antitumor activity against HER2 positive
breast-cancer cellsrsquo Biomacromolecules 14(12) pp 4248ndash4259 doi 101021bm401103t
Santander-Ortega M J et al (2006) lsquoColloidal stability of pluronic F68-coated PLGA
nanoparticles a variety of stabilisation mechanismsrsquo Journal of colloid and interface science
302(2) pp 522ndash529 doi 101016jjcis200607031
Santander-Ortega M J et al (2009) lsquoInsulin-loaded PLGA nanoparticles for oral
administration an in vitro physico-chemical characterizationrsquo Journal of biomedical
nanotechnology 5(1) pp 45ndash53 doi 101166jbn2009022
Santander-Ortega M J et al (2010) lsquoNanoparticles made from novel starch derivatives for
transdermal drug deliveryrsquo Journal of controlled release official journal of the Controlled
Release Society 141(1) pp 85ndash92 doi 101016jjconrel200908012
147
Santander-Ortega Manuel J Lozano-Loacutepez M V et al (2010) lsquoNovel core-shell lipid-
chitosan and lipid-poloxamer nanocapsules Stability by hydration forcesrsquo Colloid and
Polymer Science 288(2) pp 159ndash172 doi 101007s00396-009-2132-y
Santander-Ortega Manuel J Csaba N et al (2010) lsquoProtein-loaded PLGA-PEO blend
nanoparticles Encapsulation release and degradation characteristicsrsquo Colloid and Polymer
Science 288(2) pp 141ndash150 doi 101007s00396-009-2131-z
Santander-Ortega M J et al (2011) lsquoChitosan nanocapsules Effect of chitosan molecular
weight and acetylation degree on electrokinetic behaviour and colloidal stabilityrsquo Colloids
and surfaces B Biointerfaces 82(2) pp 571ndash580 doi 101016jcolsurfb201010019
Santander-Ortega M J Bastos-Gonzalez D and Ortega-Vinuesa J L (2007)
lsquoElectrophoretic mobility and colloidal stability of PLGA particles coated with IgGrsquo Colloids
and surfaces B Biointerfaces 60(1) pp 80ndash88 doi 101016jcolsurfb200706002
Santo V E et al (2012) lsquoFrom nano- to macro-scale nanotechnology approaches for
spatially controlled delivery of bioactive factors for bone and cartilage engineeringrsquo
Nanomedicine (London England) 7(7) pp 1045ndash1066 doi 102217nnm1278
Sapkota G et al (2007) lsquoBalancing BMP signaling through integrated inputs into the Smad1
linkerrsquo Molecular cell 25(3) pp 441ndash454 doi 101016jmolcel200701006
Schrier J A et al (2001) lsquoEffect of a freeze-dried CMCPLGA microsphere matrix of rhBMP-
2 on bone healingrsquo AAPS PharmSciTech 2(3) p E18 doi 101208pt020318
Schrier J A and DeLuca P P (2001) lsquoPorous bone morphogenetic protein-2 microspheres
polymer binding and in vitro releasersquo AAPS PharmSciTech 2(3) p E17 doi
101208pt020317
Schwendeman S P et al (2014) lsquoInjectable controlled release depots for large moleculesrsquo
Journal of controlled release official journal of the Controlled Release Society 190 pp 240ndash
253 doi 101016jjconrel201405057
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Shankarayan R Kumar S and Mishra P (2013) lsquoDifferential permeation of piroxicam-
loaded PLGA micronanoparticles and their in vitro enhancementrsquo Journal of Nanoparticle
Research 15(3) doi 101007s11051-013-1496-6
Shim Y B et al (2016) lsquoFabrication of hollow porous PLGA microspheres using sucrose for
controlled dual delivery of dexamethasone and BMP2rsquo Journal of Industrial and Engineering
Chemistry 37 pp 101ndash106 doi 101016jjiec201603014
Siafaka P I et al (2016) lsquoSurface Modified Multifunctional and Stimuli Responsive
Nanoparticles for Drug Targeting Current Status and Usesrsquo International journal of
molecular sciences 17(9) doi 103390ijms17091440
Sieber C et al (2009) lsquoRecent advances in BMP receptor signalingrsquo Cytokine amp growth factor
reviews 20(5ndash6) pp 343ndash355 doi 101016jcytogfr200910007
Silva G A et al (2007) lsquoMaterials in particulate form for tissue engineering 2 Applications
in bonersquo Journal of tissue engineering and regenerative medicine 1(2) pp 97ndash109 doi
101002term1
Simmonds M C et al (2013) lsquoSafety and effectiveness of recombinant human bone
morphogenetic protein-2 for spinal fusion a meta-analysis of individual-participant datarsquo
Annals of internal medicine 158(12) pp 877ndash889 doi 1073260003-4819-158-12-
201306180-00005
Sneh-Edri H Likhtenshtein D and Stepensky D (2011) lsquoIntracellular targeting of PLGA
nanoparticles encapsulating antigenic peptide to the endoplasmic reticulum of dendritic
cells and its effect on antigen cross-presentation in vitrorsquo Molecular pharmaceutics 8(4)
pp 1266ndash1275 doi 101021mp200198c
Spagnoli D B and Marx R E (2011) lsquoDental implants and the use of rhBMP-2rsquo Dental clinics
of North America 55(4) pp 883ndash907 doi 101016jcden201107014
Srinivasan C et al (2005) lsquoEffect of additives on encapsulation efficiency stability and
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bioactivity of entrapped lysozyme from biodegradable polymer particlesrsquo Journal of
microencapsulation 22(2) pp 127ndash138 doi 10108002652040400026400
Sturesson C and Carlfors J (2000) lsquoIncorporation of protein in PLG-microspheres with
retention of bioactivityrsquo Journal of controlled release official journal of the Controlled
Release Society 67(2ndash3) pp 171ndash178 doi 101016s0168-3659(00)00205-4
Sun D (2016) lsquoEffect of Zeta Potential and Particle Size on the Stability of SiO2 Nanospheres
as Carrier for Ultrasound Imaging Contrast Agentsrsquo Int J Electrochem Sci pp 8520ndash8529
Tan J S et al (1993) lsquoSurface modification of nanoparticles by PEOPPO block copolymers
to minimize interactions with blood components and prolong blood circulation in ratsrsquo
Biomaterials 14(11) pp 823ndash833 doi 1010160142-9612(93)90004-l
Tian Z et al (2012) lsquoSynthesis and characterization of UPPE-PLGA-rhBMP2 scaffolds for
bone regenerationrsquo Journal of Huazhong University of Science and Technology Medical
sciences = Hua zhong ke ji da xue xue bao Yi xue Ying De wen ban = Huazhong keji daxue
xuebao Yixue Yingdewen ban 32(4) pp 563ndash570 doi 101007s11596-012-0097-4
Torcello-Goacutemez A et al (2011) lsquoAdsorption of antibody onto Pluronic F68-covered
nanoparticles Link with surface propertiesrsquo Soft Matter 7(18) pp 8450ndash8461 doi
101039c1sm05570d
Torrecillas-Martinez L et al (2013) lsquoEffect of rhBMP-2 upon maxillary sinus augmentation
a comprehensive reviewrsquo Implant dentistry 22(3) pp 232ndash237 doi
101097ID0b013e31829262a8
Tran M-K Swed A and Boury F (2012) lsquoPreparation of polymeric particles in CO(2)
medium using non-toxic solvents formulation and comparisons with a phase separation
methodrsquo European journal of pharmaceutics and biopharmaceutics official journal of
Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik eV 82(3) pp 498ndash507 doi
101016jejpb201208005
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Tsuji K et al (2006) lsquoBMP2 activity although dispensable for bone formation is required
for the initiation of fracture healingrsquo Nature genetics 38(12) pp 1424ndash1429 doi
101038ng1916
Tsuji K et al (2008) lsquoBMP4 is dispensable for skeletogenesis and fracture-healing in the
limbrsquo The Journal of bone and joint surgery American volume 90 Suppl 1 pp 14ndash18 doi
102106JBJSG01109
Tsuji K et al (2010) lsquoConditional deletion of BMP7 from the limb skeleton does not affect
bone formation or fracture repairrsquo Journal of orthopaedic research official publication of
the Orthopaedic Research Society 28(3) pp 384ndash389 doi 101002jor20996
Urist M R (1965) lsquoBone formation by autoinductionrsquo Science (New York NY) 150(3698)
pp 893ndash899 doi 101126science1503698893
Vasir J K and Labhasetwar V (2007) lsquoBiodegradable nanoparticles for cytosolic delivery
of therapeuticsrsquo Advanced drug delivery reviews 59(8) pp 718ndash728 doi
101016jaddr200706003
Vo T N Kasper F K and Mikos A G (2012) lsquoStrategies for controlled delivery of growth
factors and cells for bone regenerationrsquo Advanced drug delivery reviews 64(12) pp 1292ndash
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Wallace S S and Froum S J (2003) lsquoEffect of maxillary sinus augmentation on the survival
of endosseous dental implants A systematic reviewrsquo Annals of periodontology 8(1) pp
328ndash343 doi 101902annals200381328
Wan F and Yang M (2016) lsquoDesign of PLGA-based depot delivery systems for
biopharmaceuticals prepared by spray dryingrsquo International journal of pharmaceutics
498(1ndash2) pp 82ndash95 doi 101016jijpharm201512025
Wang E A et al (1990) lsquoRecombinant human bone morphogenetic protein induces bone
formationrsquo Proceedings of the National Academy of Sciences of the United States of America
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87(6) pp 2220ndash2224 doi 101073pnas8762220
Wang H et al (2012) lsquoThe use of micro- and nanospheres as functional components for
bone tissue regenerationrsquo Tissue engineering Part B Reviews 18(1) pp 24ndash39 doi
101089tenTEB20110184
Wang Y et al (2015) lsquoPLGAPDLLA core-shell submicron spheres sequential release
system Preparation characterization and promotion of bone regeneration in vitro and in
vivorsquo Chemical Engineering Journal 273 pp 490ndash501 doi 101016jcej201503068
Wege H A Holgado-Terriza J A and Cabrerizo-Vilchez M A (2002) lsquoDevelopment of a
constant surface pressure penetration langmuir balance based on axisymmetric drop shape
analysisrsquo Journal of colloid and interface science 249(2) pp 263ndash273 doi
101006jcis20028233
Wheeler S L (1997) lsquoSinus augmentation for dental implants the use of alloplastic
materialsrsquo Journal of oral and maxillofacial surgery official journal of the American
Association of Oral and Maxillofacial Surgeons 55(11) pp 1287ndash1293 doi 101016s0278-
2391(97)90186-5
White L J et al (2013) lsquoAccelerating protein release from microparticles for regenerative
medicine applicationsrsquo Materials science amp engineering C Materials for biological
applications 33(5) pp 2578ndash2583 doi 101016jmsec201302020
Wozney J M (1992) lsquoThe bone morphogenetic protein family and osteogenesisrsquo Molecular
reproduction and development 32(2) pp 160ndash167 doi 101002mrd1080320212
Xia Y et al (2013) lsquoProtein encapsulation in and release from monodisperse double-wall
polymer microspheresrsquo Journal of pharmaceutical sciences 102(5) pp 1601ndash1609 doi
101002jps23511
Xiong S et al (2011) lsquoCellular uptake of Poly-(DL-lactide-co-glycolide) (PLGA)
nanoparticles synthesized through solvent emulsion evaporation and nanoprecipitation
152
methodrsquo Biotechnology journal 6(5) pp 501ndash508 doi 101002biot201000351
Xu Y et al (2017) lsquoPolymer degradation and drug delivery in PLGA-based drug-polymer
applications A review of experiments and theoriesrsquo Journal of biomedical materials
research Part B Applied biomaterials 105(6) pp 1692ndash1716 doi 101002jbmb33648
Yallapu M M et al (2010) lsquoFabrication of curcumin encapsulated PLGA nanoparticles for
improved therapeutic effects in metastatic cancer cellsrsquo Journal of colloid and interface
science 351(1) pp 19ndash29 doi 101016jjcis201005022
Yameen B et al (2014) lsquoInsight into nanoparticle cellular uptake and intracellular
targetingrsquo Journal of controlled release official journal of the Controlled Release Society 190
pp 485ndash499 doi 101016jjconrel201406038
Yang Y Y Chia H H and Chung T S (2000) lsquoEffect of preparation temperature on the
characteristics and release profiles of PLGA microspheres containing protein fabricated by
double-emulsion solvent extractionevaporation methodrsquo Journal of controlled release
official journal of the Controlled Release Society 69(1) pp 81ndash96 doi 101016s0168-
3659(00)00291-1
Yang Y Y Chung T S and Ng N P (2001) lsquoMorphology drug distribution and in vitro
release profiles of biodegradable polymeric microspheres containing protein fabricated by
double-emulsion solvent extractionevaporation methodrsquo Biomaterials 22(3) pp 231ndash
241 doi 101016s0142-9612(00)00178-2
Yilgor P et al (2009) lsquoIncorporation of a sequential BMP-2BMP-7 delivery system into
chitosan-based scaffolds for bone tissue engineeringrsquo Biomaterials 30(21) pp 3551ndash3559
doi 101016jbiomaterials200903024
Yilgor P Hasirci N and Hasirci V (2010) lsquoSequential BMP-2BMP-7 delivery from
polyester nanocapsulesrsquo Journal of biomedical materials research Part A 93(2) pp 528ndash
536 doi 101002jbma32520
153
Zhang H-X et al (2016) lsquoIn vitro and in vivo evaluation of calcium phosphate composite
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Pharmaceutical research 26(7) pp 1561ndash1580 doi 101007s11095-009-9897-z
154
10ANEXO MATERIAL SUPLEMENTARIO
httprsbwebnihgovij
155
156
11ANEXO DE PUBLICACIONES
Review ArticleBone Regeneration from PLGA Micro-Nanoparticles
Inmaculada Ortega-Oller1 Miguel Padial-Molina1 Pablo Galindo-Moreno1
Francisco OrsquoValle2 Ana Beleacuten Joacutedar-Reyes3 and Jose Manuel Peula-Garciacutea34
1Department of Oral Surgery and Implant Dentistry University of Granada 18011 Granada Spain2Department of Pathology School of Medicine and IBIMER University of Granada 18012 Granada Spain3Biocolloid and Fluid Physics Group Department of Applied Physics University of Granada 18071 Granada Spain4Department of Applied Physics II University of Malaga 29071 Malaga Spain
Correspondence should be addressed to Jose Manuel Peula-Garcıa jmpeulaumaes
Received 27 March 2015 Accepted 4 June 2015
Academic Editor Hojae Bae
Copyright copy 2015 Inmaculada Ortega-Oller et al This is an open access article distributed under the Creative CommonsAttribution License which permits unrestricted use distribution and reproduction in any medium provided the original work isproperly cited
Poly-lactic-co-glycolic acid (PLGA) is one of the most widely used synthetic polymers for development of delivery systems fordrugs and therapeutic biomolecules and as component of tissue engineering applications Its properties and versatility allow it tobe a reference polymer in manufacturing of nano- and microparticles to encapsulate and deliver a wide variety of hydrophobic andhydrophilic molecules It additionally facilitates and extends its use to encapsulate biomolecules such as proteins or nucleic acidsthat can be released in a controlled wayThis review focuses on the use of nanomicroparticles of PLGA as a delivery system of oneof the most commonly used growth factors in bone tissue engineering the bone morphogenetic protein 2 (BMP2) Thus all theneeded requirements to reach a controlled delivery of BMP2 using PLGA particles as a main component have been examinedTheproblems and solutions for the adequate development of this system with a great potential in cell differentiation and proliferationprocesses under a bone regenerative point of view are discussed
1 Introduction
Bone regeneration is one of the main challenges facing us inthe daily clinic Immediately after a tooth extraction normalbiological processes remodel the alveolar bone limiting insome cases the possibility of future implant placementDifferent strategies for the preservation of that bone havebeen explored in recent years Other conditions such astrauma tumor resective surgery or congenital deformitiesrequire even higher technical and biological requirementsto generate the necessary bony structure for the occlusalrehabilitation of the patient To overcome these anatomicallimitations in terms of bone volume different approacheshave been proposed to either improve the implant osteoin-tegration or to augment the bone anatomy where it will beplaced [1 2] Autogenous bone graft is still considered theldquogold standardrdquo due to its osteogenic osteoconductive andosteoconductive properties [3 4] However it also presentsseveral limitations including the need for a second surgery
limited availability and morbidity in the donor area [5]Therefore other biomaterials such as allogeneic grafts withosteoconductivity and osteoinductive capacities [6 7] andxenogeneic grafts [8 9] and alloplastic biomaterials [10]with osseoconductive potential were proposed All thesematerials although acceptable are not suitable in manyconditions and usually require additional consideration inthe decision process [11] Additionally the bone quantity andquality that can be obtained with these materials are oftenlimited
The use of bioactive molecules alone or in combina-tion with the previously described materials has thereforebecome amajor area of interest thanks to their high potentialWhen using this kind of procedures it is important toconsider (1) the delivery method and (2) the molecule itselfBioactive molecules can be transported into the defect areaas a solution or a gel embedded in sponges adhered to solidscaffolds and more recently included in particles of differentsizes Using these methods PDGF (platelet-derived growth
Hindawi Publishing CorporationBioMed Research InternationalVolume 2015 Article ID 415289 18 pageshttpdxdoiorg1011552015415289
2 BioMed Research International
factor) FGF (fibroblast growth factor) IGF (insulin growthfactor) Runx2 Osterix (Osx) LIM domain mineralizationprotein (LMP) BMP (bone morphogenic protein) and morerecently periostin have been proposed as potential candidatesfor regeneration procedures within the oral cavity includingbone and periodontal tissues [12 13] These molecules havebeen tested alone or in combination with stem cells [14] usingseveral in vitro and in vivo strategies [15]
Consequently within the context of this review we intendto review the delivery methods of bioactive molecules withthe purpose of bone regeneration with a particular focus onpolymeric nanomicroparticles especially those with PLGAas main component to encapsulate the growth factor BMP-2 An overview of the biological functions of bone morpho-genetic proteins and an analysis of the different parametersaffecting the physicochemical properties of these systems arepresented Synthesis method particle size and morphologyuse of stabilizers and their incidence in the colloidal sta-bility protective function and surface functionality will bediscussed In addition we explore the different strategies thatcan be used to optimize the encapsulation efficiency andrelease kinetics main parameters that determine the correctdevelopment of polymeric carriers used in tissue-engineeredbone processes
2 BMPs Action and Regulation
For bone regeneration in particular bone morphogeneticgrowth factors (BMP) are probably the more tested groupof molecules Since 1965 when Urist [16] showed that theextracted bone BMPs could induce bone and cartilage forma-tion when implanted in animal tissue an increasing numberof reports have tested its in vivo application and biologicalfoundation when used in bone defects [17ndash19] BMPs aremembers of the TGF-120573 superfamily of proteins [20] TheBMP family of proteins groups more than 20 homodimericor heterodimericmorphogenetic proteins which functions inmany cell types and tissues not all of them being osteogenic[21] BMPs can be divided into 4 subfamilies based on theirfunction and sequence being BMP-2 BMP-4 and BMP-7 the ones with osteogenic potential [21] The actions ofBMPs include chondrogenesis osteogenesis angiogenesisand extracellular matrix synthesis [22] Within this fam-ily of proteins BMP-2 has been the most studied It hasosteoinductive properties that promote the formation of newbone by initiating stimulating and amplifying the cascadeof bone formation through chemotaxis and stimulationof proliferation and differentiation of the osteoblastic celllineage [5 17 19 20]The absence of it as studied in knockoutmodels leads to spontaneous fractures that do not heal withtime [23] In fact other models have demonstrated that theabsence of either BMP-4 [24] or BMP-7 [25] do not lead tobone formation and function impairmentwhich demonstratethe compensatory effect produced by BMP-2 alone [26]
Many cell types in bone tissue produce BMPs includingosteoprogenitor cells osteoblasts chrondrocytes plateletsand endothelial cells This secreted BMP is then stored in theextracellular matrix where it mostly interacts with collagentype IV [27] During the repair and remodeling processes
BMPs
BMPR-I BMPR-II
Smad 1 Smad 5
Smad 8Smad 4
Runx2 Dlx5 Osterix
Osteogenesis
Bone resorption
Figure 1 Schematic representation of the main BMP molecularpathway to osteogenesis BMPs interact with cell surface receptorsI and II to activate Smads 1 5 and 8 These activated Smads activateSmad 4 All together as a protein complex activate Runx2 Dlx5 andOsterix
osteoclast resorptive activity induces the release of BMPs tothe medium so that they are suspended and can interact withnearby cells to initiate the subsequent osteogenic process [28]
A BMP in the extracellular matrix binds to cell surfacereceptors BMPR-I and BMPR-II and activates the Smadcytoplasmic proteins or the MAPK pathway [29] WhenBMPR-I is activated BMPR-II is recruited and activatedas well [30] The activation of the complexes BMPR-I andBMPR-II leads to the activation of several Smads (1 5 and 8)that also activate Smad 4 and they all form protein complexesthat are transported into the nucleus where Runx2 Dlx5and Osterix genes (important in osteogenesis) are activated[26 27] (Figure 1) Similarly when the MAPK pathwayis activated it leads to induction of Runx2 transcriptionand therefore to bone differentiation [31] A number ofextracellular and intracellular antagonists have also beendescribed including noggin chordin and gremlin or Smads6 7 and 8b respectively [32]
21 Clinical Use of BMP-2 Today the BMP-2 is commerciallyavailable under different brand names and concentrations Itusually consists of a collagen absorbable sponge embeddedwith recombinant human BMP-2 In 2002 it was approvedby the FDA as an alternative of autogenous bone graftingin anterior lumbar interbody fusion [33] Later in 2007 theFDA approved the use of rhBMP-2 as an alternative forautogenous bone grafting in the increase of the alveolar crestdefects associated with the tooth extraction maxillary sinuspneumatization [33]
Beside the applications in spine clinical studies wherevery high concentrations are used (AMPLIFY rhBMP-240mg) clinical studies have supported its use in the oral
BioMed Research International 3
cavity BMPs have been used in periodontal regenerationbone healing implant osteointegration oral surgery withorthodontic purposes bone pathology sequel repair distrac-tion osteogenesis and endodontic reparative surgery [28 34]However it has shownmore promising results in cases whereonly bone tissue is to be regenerated including preimplantsite development sinus lift vertical and horizontal ridgeaugmentation and dental implant wound healing [35] In thissense it has been shown that the use of rhBMP-2 induced theformation of bone suitable for placement of dental implantsand their osteointegration [36] Furthermore it appears thatthe newly formed bone has similar properties to the nativebone and is therefore capable of supporting denture occlusalforces [37] In the particular case of sinus lifting where bonedeficiency is greater and therefore supportive therapies canbe more helpful a recent meta-analysis found a total of 3human studies and 4 animal trials (Table 1) [38] In summarythe included studies concluded that rhBMP-2 induces newbone formation with comparable bone quality and quantityof newly formed bone to that induced by autogenous bonegraft In some cases even higher bone quality and quantityhave been reported [39]
Conversely recent studies report severe complicationsafter its use [61] Even more high doses have also associatedwith carcinogenic effects which led the authors to emphasizethe need for better guidelines in BMP clinical use [62] Notso drastic recent studies are highlighting the negative sideeffects and risks of its application making high emphasison potential bias of nonreproducible industry sponsoredresearch especially when used in spinal fusion [44 63 64]The use of rhBMP-2 has been shown to increase the risks forwound complications and dysphagia with high effectivenessand harms misrepresentation through selective reportingduplicate publication and underreporting [44] Specificallyin oral bone regenerative applications a report in sinus liftconcluded that the use of BMP-2 promotes negative effectson bone formation when combined with anorganic bovinebone matrix versus anorganic bovine bone alone [41] incontrast with previous reports and reviews [38] Takingtogether this information it can be concluded that it is ofextreme importance to be careful with the clinical use of newproducts avoiding off-label applications It is also importantto highlight the need for more and better clinical research
To overcome these limitations new strategies such asthe use of ex vivo BMP-2-engineered autologous MSCs [65]encapsulation of the protein in different biomaterials ordelivery by gene therapy are being explored in recent years
The development of these technologies is based on somebiological facts In vitro effects of BMPs are observed at verylow dosages (5ndash20 ngmL) although current commerciallyavailable rhBMPs are used in large dosages (up to 40mg ofsome products) [28] This is probably due to an intense pro-teolytic consumption during the early postsurgical phases Itis important to know the proper sequence of biological eventsthat lead to normal tissue healing Then this knowledge canbe used to intervene at the specific time frame where ourtherapy is intended to act [15] Effective bone formation asdescribed above is a sequential processTherefore the induc-tive agent should be delivered at a maintained concentration
during a timeframe In this sense as in many other processesin medicine it has been recently demonstrated that long-term release of BMP-2 is more effective than short-term overa range of doses [51] It is also important to note that therole of other molecular pathways and crosstalk between thedifferent components playing in bone regeneration is notperfectly understood yet and therefore more research hasto be conducted
What is known so far in summary is that BMPs specif-ically BMP-2 is of utility for promoting bone regeneration[28] However the currently FDA-approved BMP-2 deliverysystem (INFUSE Medtronic Sofamor Danek Inc) presentsimportant limitations [66] Firstly protein is quickly inacti-vatedTherefore its biological action disappears maybe evenbefore the blood clot that forms after the surgery is beingorganized Second the recombinant protein is delivered inan absorbable collagen sponge Thus the distribution ofthe BMP in a liquid suspension embedded into a collagensponge makes it impossible to be certain that the protein isreaching the ideal target Therefore where when and forhow long a dose of BMP-2 is reached (determined by thedelivery method) are important factors Because of that newforms of BMP-2 delivery are being developed These newtechnologies have to guarantee a higher half-life of the proteinand a stepped release to increase the effects on the desiredcell targets The biotechnology opens the door to be able toprovide a solution to these limitations
Biodegradable nanoparticles (nanospheres and nanocap-sules) have developed as a promising important tool forthe delivery of macromolecules via parenteral mucous andtopical applications [67ndash70] Well-established biodegradablepolymers such as poly(acid D L-lactic) or poly(D L-lactic-co-glycolic) have been widely used in the preparation ofnanoparticles in recent decades because of its biocompati-bility and full biodegradability [71] However it is knownthat certain macromolecules such as proteins or peptidesmay lose activity during their encapsulation storage deliveryand release [72] To overcome this problem the addition ofstabilizers such as oxide polyethylene (PEO) or the coencap-sulation with other macromolecules and its derivatives seemto be a promising strategy
3 Polymeric Colloidal Particles to EncapsulateHydrophilic Molecules
Generally polymeric colloidal particles are hard systemswith a homogeneous spherical shape composed by naturalor synthetic polymers In order to encapsulate hydrophilicmolecules as proteins or nucleic acids it is necessary to opti-mize the polymeric composition and the synthesis methodIn this process a high encapsulation efficiency maintenanceof the biological activity of the encapsulated biomolecule andobtaining of an adequate release pattern have to be achieved[73ndash75] Several delivery systems of BMP2 (and other growthfactors GFs) using polymeric particles have been describedin the literature Most of them are microparticulated systemsusing the biocompatible and biodegradable PLGA copoly-mer as main component [76 77] Taking into account theincorporation of BMP2 to the carrier system encapsulation
4 BioMed Research InternationalTa
ble1
Sum
maryo
fclin
icalandanim
alstu
diesusingB
MP-2for
sinus
floor
elevatio
n(adapted
from[38])Th
eincludedstu
diesoverallcon
cludedthatrhBM
P-2ind
ucesnewbo
neform
ation
with
comparableb
oneq
ualityandqu
antityof
newlyform
edbo
neto
thatindu
cedby
autogeno
usbo
negraft
Reference
Stud
ydesig
nFo
llow-up
(mon
ths)
Species
(sub
jects)
Coreb
iopsy
harvestin
g(m
onths)
Graftmaterial
New
lyform
edbo
neBo
neheight
gain
(mm)
Bone
width
gain
(mm)
Bone
density
(mgmL)
Immun
erespon
seHistolog
y
Boyn
eetal
2005
[37]
RCT
52Hum
an(48)
6ndash11
075
mgmL
rhBM
P-2AC
S
NA
1129
Crest202
Midpo
int854
Apical118
684
Non
eNA
150m
gmL
rhBM
P-2AC
S047
Crest19
8Midpo
int78
0Ap
ical1078
134
Autogeno
usbo
negraft
autogenou
sbo
negraft
+allogeneicbo
negraft
1016
Crest466
Midpo
int
1017
Apical1056
350
Triplettetal
2009
[40]
RCT
58Hum
an(160)
6
150m
gmL
rhBM
P-2AC
S
NA
783plusmn352
NA
200
Non
e
Rich
vascular
marrowspaceh
ighin
cellu
larc
ontent
Autogeno
usbo
negraft
(iliacc
rest
tibia
ororalcavity)
autogeno
usbo
negraft
+allogeneic
bone
graft
946plusmn411
283
Oste
oclasts
stillpresenthigh
erfib
rous
tissue
Kaoetal2012
[41]
Prospective
6ndash9
Hum
an(22)
6ndash9
rhBM
P-2AC
S+
ABB
1604plusmn74
5
NA
NA
NA
Non
e
Fewer
ABB
particleslessnewlyform
edbo
ne(w
oven
andmatureb
ones
tructure)
ABB
2485plusmn582
MoreA
BBparticlesrem
aining
higher
newlyform
edbo
ne(w
oven
andmatured
bone
structure)
Nevinse
tal
1996
[36]
Prospective
12Goat(6)
12
rhBM
P-2AC
S
NA
NA
NA
NA
Non
e
Dense
isolatedtrabeculae
andbo
nemarrowoste
oblastandosteoclasts
no
corticalbo
ne
ACSBu
ffer
Collageno
usconn
ectiv
etissuen
oevidence
ofinflammation
noneo-osteogenesis
Hanisc
hetal
1997
[42]
RCT
24Non
human
prim
ate(12)
24rhBM
P-2AC
SNA
60plusmn03
NA
144plusmn29
NA
New
lyform
edbo
neindisting
uishable
from
resid
ualbon
eAC
S26plusmn03
139plusmn46
Wadae
tal
2001
[43]
Prospective
8Ra
bbit(10)
8rhBM
P-2AC
S224plusmn44
NA
NA
NA
NA
Cortic
albo
neform
ationin
both
grou
ps
trabeculae
with
clear
lamellarstructure
weree
mbedd
edin
fatty
marrow
Autogeno
usbo
negraft
(iliacc
rest)
219plusmn45
Leee
tal2013
[39]
Prospective
8Mini-p
ig(8)
8rhBM
P-2AC
SNA
93plusmn05
NA
519plusmn3
NA
New
lyform
edcancellous
bonenew
bone
continuo
uswith
resid
entb
onewoven
bone
infib
rovascular
andfatty
marrow
Autogeno
usbo
negraft
(iliacc
rest)
86plusmn07
329plusmn25
Irregu
lara
ndvaria
bleb
onea
mon
gdifferent
subjects
NAnot
availableRC
Trand
omized
clinicaltrialAC
Sabsorbablecollagenspon
geA
BBano
rganicbo
vine
bone
BioMed Research International 5
Hydrophilic biomolecules stabilizers
Organicphase
PLGA and stabilizers(surfactant other polymers) in
DCM acetone or EtAc
Mixture underagitationsonication
Ethanol water surfactants
Antibody
Core
PLGA BSABMP-2
Surface
Surfactant
MicronanosphereMicronanocapsule
ImmunoparticleDirected delivery
Organic solventextraction under
vacuumAqueous phase w1
First w1oemulsion
emulsion
Second polarphase w2
Final w1ow2
120
80
40
150mL
120
80
40
150mL
Figure 2 Double emulsion procedure (wateroilwater emulsion W1OW
2) to obtain PLGA micronanoparticles Depending on the
synthesis conditions (stabilizers solvents and mixing procedure) it is possible to obtain micro-nanospheres with a uniformmatrix or micro-nanocapsules with a core-shell structure Immunoparticles used for directed delivery can be obtained by attaching specific antibodymoleculeson the particle surface
is preferred to absorption because the growth factors aremore protected against environmental factors in the mediumand may have better control over the delivery and release toachieve the desired concentrations in specific site and time[78]
Normally if the GFs are related with bone regenerationprocesses nano-microparticles are trapped in a second sys-tem as hydrogels or tissue engineering scaffolds which alsoplay an important role in the release profile of GFs fromthese particles [78] The nano-microparticles have allowedthe development of multiscale scaffold thereby facilitatingcontrol of the internal architecture and adequate patterns ofmechanical gradients of cells and signaling factors [79]
All steps from the synthesis method and its characteris-tics the encapsulation process or the final surface modifica-tion for a targeted delivery determine the characteristics ofthese systems and their main goal the controlled release ofbioactive GFs
31 Synthesis Methods It is possible to found several pro-cedures to encapsulate hydrophilic molecules as proteinsor nucleic acids in polymeric nanomicroparticles Phase
separation [80] or spray drying [81] techniques have beenreported to encapsulate hydrophilic molecules However inthe case of proteins the most normally used procedureto encapsulate them into PLGA micro- and nanoparticlesis the double-emulsion (wateroilwater WOW) solventevaporation technique [75 82] A schematic description ofthis technique is presented in Figure 2 In a general wayPLGA is dissolved in an organic solvent and emulsified usingmechanical agitation or sonication with water containingan appropriate amount of protein Thus a primary wateroil(WO) emulsion is obtained In the second phase thisemulsion is poured into a large polar phase leading to animmediate precipitation of the particles as a consequenceof the polymer shrinkage around droplets of the primaryemulsion This phase may be composed of a water solutionof a stabilizer (surfactant) or ethanol-water mixtures [8384] After stirring the organic solvent is rapidly extractedby evaporation under vacuum A wide list of differentmodifications have been tested in this procedure in order toobtain a micronanocarrier system with adequate colloidalstability high encapsulation efficiency adequate bioactivityand finally a long-time release profilewith low ldquoinitial burstrdquo
6 BioMed Research International
The goal is to avoid a high amount of protein (gt60) beingreleased very quickly (24 hours) which is one of the biggestproblems of a controlled release system [76]
32 Organic Solvent Hans and Lowman show differentexamples of organic solvents used in multiple emulsionprocesses Normally dichloromethane (DMC) ethyl acetateacetone and their mixtures can be used [82] In the first stepa good organic solvent with low water solubility to facilitatethe emulsification process and low boiling point for an easyevaporation would be the election However the structureof the encapsulated protein molecules can be affected anddenaturation processes and loss of biological activity appearwhen they interact with a typical organic solvent as DMC[73] Ethyl acetate on the other hand exerts less denaturatingeffects with a lower incidence on the bioactivity of theencapsulated proteins [85]
Other important factors related with the organic solventare their physical properties that affect how the polymertails self-organize in the shell of the emulsion droplets andmodify the nanoparticle morphology and the encapsulationefficiency [86] In this way a higher water solubility ofthe organic solvent that is ethyl acetate favors a rapidsolvent removal Additionally the solvent removal rate canbe controlled by adjusting the volume of the polar phase aswell as the shear stress during the second emulsification stepAn increase of these two parameters increases the diffusionrate of ethyl acetate from primary microparticles to outeraqueous phase resulting in their rapid solidification [87] Italso enhances the encapsulation efficiency andminimizes thecontact-time between protein molecules and organic solvent[88] obtaining at the same time a lower burst effect and aslower drug release from the microparticles [87]
33 Particle Size and Morphology Particle size is an impor-tant parameter and one of the main goals of the deliverypolymeric system Microspheres from a few micrometers upto 100 120583m are suitable for oral delivery mucosal adhesion orinside scaffold use that is for bone regeneration Nanoscaledimension of the carrier offers enhanced versatility whencompared with particles of larger size This is due to thefact that they have higher colloidal stability improved dis-persibility and bioavailability more reactive surface and alsocan deliver proteins or drugs inside and outside of thecorresponding cells [89] BMP2 promotes bone formationand induces the expression of other BMPs and initiates thesignaling pathway from the cell surface by binding to twodifferent surface receptors [22] Therefore the BMP2 carrierparticles must release it into the extracellular medium Sincecellular intake of PLGAnanoparticles is very fast the intakingprocess can be limited by an increase in size from nano-to microparticles [90] However the interaction betweenparticles and cells is strongly influenced by particle size Ifcell internalization is desired the particle must be comprisedin the submicron scale at an interval between 2 and 500 nm[91] Moreover this size is needed for a rapid distributionafter parenteral administration in order to reach differenttissues through different biological barriers In addition
Figure 3 Scanning electron microscopy (SEM) photography ofPLGA nanoparticles obtained by a double emulsion emulsificationprocedureThis systemwith spherical shape low polydispersity andnanoscopic scale shows the intended properties for an adequatephysiological distribution and cell internalization
the intake by macrophages is minimized with a diameterof nanoparticles under 200 nm and even smaller [82 92]As discussed by Yang et al [93] slight modifications ofthe synthesis procedure can suppose drastic effects on thesize or particle morphology and therefore in the proteinencapsulation efficiency and kinetic release
In double emulsion processes the first emulsificationstep largely determines the particle size while the secondemulsification step characterized by the solvent eliminationand polymer precipitation mainly affects the particle mor-phology [86] However the use of surfactant solutions asthe polar medium of the second emulsification process andthe volume ratio between organic and polar phases in thisstep has shown an important influence in the final size [94]Therefore the correct election of the organic solvent thepolymer concentration the addition of surfactant and theemulsification energy allow controlling the size of the system
The incorporation of poloxamers (F68) in the organicsolvent of the primary emulsification helps to increase thecolloidal stability of the first dispersion by being placedat the wateroil interface This reduces the particle size incomparison with pure PLGA nanoparticles in which theonly stability source comes from electric charge of thecarboxyl groups of the PLGA [95] It is normal to obtainspherical micronanospheres with a polymeric porous coreA typical SEM micrograph of PLGA nanoparticles obtainedby WOW emulsion using a mixture of organic solvents(DCMacetone) and ethanolwater as second polar mediumis shown in Figure 3 in which the spherical shape anduniform size distribution are the main characteristics Theouter polymeric shell in the second emulsification steppushed the water droplets to the inner core according to theirsolidification process [96] This process allows producingparticles like capsules with a core-shell structure in whichthe inner core has a low polymer density Figure 4 shows atypical core-shell structure in which the polymer precipitatesand shrinks around the water droplets during the solventchange of the second phase and the subsequent organicsolvent evaporation process [97] In this case the process of
BioMed Research International 7
(a) (b)
Figure 4 PLGApoloxamers188 blend nanoparticles (a) Scanning transmission electron microscopy (STEM) photography (b) scanningelectron microscopy (SEM) photography STEM technique allows the analysis of the nanoparticle structure with an internal region with alow polymer density which is representative of nanocapsules with core-shell structure
solidification of the polymer is influenced and determined bythe miscibility of the organic solvent with the second polarphase and the removal rate
The polymeric shell often presents channels or pores asa consequence of the inner water extrusion due to osmoticforcesThis can reduce the encapsulation efficiency and favorsa fast initial leakage with the unwanted ldquoburst releaserdquo [93]This modification of internal structure of the particles isusually indicated assigning the term ldquonanosphererdquo to thesystem with a core consisting of a homogeneous polymermatrix The bioactive agent is dispersed within them whilethe core-shell structure would be similar to a ldquonanocapsulerdquowhere the biomolecule is preferably in the aqueous cavitysurrounded by the polymeric shell [78] (see Figure 2)
34 Stabilizer Agents
341 Colloidal Stability The double emulsion method nor-mally requires the presence of stabilizers in order to confercolloidal stability during the first emulsification step toprevent the coalescence of the emulsion droplets and latertomaintain the stability of the final nanomicroparticles [98]Polyvinyl alcohol (PVA) and PEO derivate as poloxamers(also named pluronics) have been used inmost cases [83 94]Others include natural surfactants such as phospholipids[99 100] In some cases it is possible to avoid surfactantsif the particles have an electrostatic stability contributionthat is from the uncapped end carboxyl groups of the PLGAmolecules [101]
As it has been previously commented PVA and polox-amers have shown their efficiency in synthetizing both nano-and microparticles affecting not only the stability of thesystems but also their size and morphology Thus a sizereduction effect has been found using PVA in the externalwater phase affecting at the same time the surface porosity
mainly in microsized particles [94] A comparative studybetween this and phospholipids (di-palmitoyl phosphaty-dilcholine DPPC) as stabilizers showed that DPPC couldbe a better emulsifier than PVA to produce nano- andmicroparticles With this method a much lower amount ofstabilizer was needed to obtain a similar size In the samestudy a higher porosity on the particle surface for the PVAemulsified nanospheres was shown [99]
On the other hand the combination of PLGA withpoloxamers has shown positive effects for the nano- andmicrosystems in terms of stability [102] The use of thesesurfactants in the first or second steps of the WOWemulsion procedure leads to different situations Thus ifpoloxamers are blended with PLGA in the organic phaseof the primary emulsification an alteration of the surfaceroughness is obtained However if these are added in theinner water phase an increase of porosity is found [83] Inaddition their inclusion in the polar phase of the secondemulsification step also generates hydrophilic roughnesssurfaces A quantification of this is shown in Figure 5 inwhich the electrophoretic mobility of both PLGA pure andPLGApluronic F68 nanoparticles is measured as a functionof the pH of the medium The observed dependence withthis parameter is a consequence of the weak acid characterof the PLGA carboxyl groups When poloxamer moleculesare present at the interface a systematic reduction ofmobilitywas found as a consequence of the increase in the surfaceroughness The hydrophilic surfactant chains spread outtowards the solvent originating a displacement of the shearplane and the consequent mobility reduction [95 101]
The final PLGA particle size is primarily controlled byelectrostatic forces and is not significantly affected by thepresence or nature of poloxamer stabilizers [101] The recog-nition of the nanocarriers by the mononuclear phagocyticsystem (MPS) can be significantly altered if the surface of
8 BioMed Research International
pH4 5 6 7 8 9
minus6
minus4
minus2
0
2
120583e
(V m
minus1
sminus1)
Figure 5 Electrophoretic mobility versus pH for PLGA nano-particles with different characteristics (998787) PLGA (◼) PLGApolox-amer188 blend and (∙) PLGA covered by Immuno-120574-globulin Thedifferent surface composition affects the electrokinetic behaviourof bare nanoparticles Surface charge values were screened by thepresence of nonionic surfactant as poloxamers or in a higherextension by the presence of antibody molecules attached on thesurface
colloidal particles is modified by using PEO block copoly-mer of the poloxamer molecules The steric barrier givenby these surfactant molecules prevents or minimizes theadsorption of plasma protein and decreases the recognitionby macrophages [103] The size of microspheres is alsounaffected by the coencapsulation of poloxamers The sys-tem containing poloxamer-PLGA blends drive to an innerstructure displaying small holes and cavities in relation withmicrospheres of pure PLGA with a compact matrix-typestructure [83]
Microparticles formulated by poloxamer in the secondpolar medium have completely different surface than thePVA ones almost without pores [94] A comparison betweendifferent poloxamers shows that the hydrophilic-lipophylicbalance (HBL) of the surfactant plays a crucial role determin-ing the surfactant-polymer interactions and controlling theporosity and roughness of the nano-microparticles [83 104]
In a similar manner to surfactants polymer character-istics like the hydrophobicity grade the molecular weightor the hydrolysis degradation rate can strongly influencethe particlemorphologyTherefore the polymer compositionof the particles greatly affects its structure and propertiesThis is why it is usual to use other polymers in order tomodify the behavior and application of the particles Inthis way polyethylene glycol (PEG) of different chain lengthis frequently used to modify the surface characteristicsWith PEG particles are more hydrophilic and with roughersurfaces which affects the MPS action by increasing thecirculating-time and half-life in vivo like the presence of PEOchains [105] Additionally PEG chains also provide colloidalstability via steric stabilization Pegylated-PLGA nano- ormicroparticles can be normally obtained by using in the
synthesis method PLGAPEG di- and triblock copolymers[58 59 75] Natural polymers as chitosan besides modifyingthe hydrophobicity-hydrophilicity ratio of the surface alsoconfer them a mucoadhesive character [106]
342 Encapsulation Efficiency and Bioactivity Furthermorethe use of stabilizers (surfactants or polymers) also influ-ences the encapsulation efficiency and the protein stabilityIn fact for the WOW solvent evaporation process thechlorinated organic solvent used for the first emulsificationcould degrade protein molecules encapsulated in this stepif they come into contact with the organicwater interfacecausing their aggregation or denaturation [107]Thepolymer-protein interaction the shear stress for the emulsificationprocess and the pH reduction derived from PLGA polymerdegradation can also produce the same situation with thesubsequent loss of biological activity of the encapsulatedbiomolecules Different strategies to prevent it have beenused For example an increase of the viscosity around proteinmolecules can help to isolate them from their microenviron-ment [108] In this way viscous products such as starch havebeen used to prevent protein instability [109] These authorscoencapsulate BMP2 with albumin inside starch microparti-cles using other biodegradable polymer poly-120576-caprolactoneinstead of PLGA The BMP2 retained its bioactivity Despitea low encapsulation rate beside an initial burst followedby an uncompleted release the amount of BMP2 neededat the beginning was lower [109] The combination of PEOsurfactants with PLGA (blended in the organic phase) canalso preserve the bioactivity of microencapsulated proteins[110] or nucleic acids [84]
However in most cases the coencapsulation of GFswith other biomolecules was the preferred strategy Therebyserum albumins (SA) have shown the capacity to limit theaggregation-destabilization of several proteins incited by thewaterorganic solvent interface of the primary emulsificationprocess [111 112] White et al encapsulated lysozyme insidePLGA-PEG microparticles In addition to the protectivefunction they also observed an important increase of theentrapment efficiency when human SA was coencapsulatedwith lysozyme and BMP2 [59] drsquoAngelo et al used heparinas stabilizer because it forms a specific complex with severalGFs stabilizes their tridimensional structure and promotestheir bioactivity An encapsulation efficiency of 35 wasincreased to 87 using bovine SA as a second stabilizer toencapsulate two natural proangiogenic growth factors insidePLGA-poloxamer blended nanoparticlesThe in vitro cellularassays showed the preservation of the biological activity ofGFs up to one month [56]
The use of more hydrophilic surfactants (poloxamers)or polymers (PEG) in the inner water phase or blendedwith PLGA in the organic phase of the primary emulsionreduces the interaction of encapsulated proteins with thehydrophobic PLGA matrix This prevents disrupting thestructure of the protein molecules and helps at the sametime to neutralize the acidity generated by the hydrolyticdegradation of the PLGA [113] In some cases the combina-tion of several stabilizers such as poloxamers trehalose andsodium bicarbonate has been shown to preserve the integrity
BioMed Research International 9
of encapsulated proteins but it also reduces the encapsulationefficiency [114]
As a general rule encapsulation efficiency increases withthe size of the particles [82] Additionally the adequate sta-bilization of the primary emulsion by amphiphilic polymersand a rapid solidification (precipitation) of polymer in thesecond step are favorable parameters for enhancing proteinentrapment efficiency in the WOW emulsion technique[87]
The tendency of BMP2 to interact with hydrophobicsurfacesmay decrease the loss of encapsulated protein duringthe extraction of the solvent phase This favors a higherentrapment but it lowers the later extraction [58] An optimalprotein encapsulation is obtained when pH of the internaland external water phases is near the isoelectric point of theprotein [92] Blanco and Alonso [83] observed a reductionin the protein encapsulation efficiency when poloxamer wascoencapsulated in the primary emulsion This highlights themain role played by the protein-polymer interaction in theencapsulation efficiency and the later release process How-ever too much emulsifier may also result in a reduction ofthe encapsulation efficiency [99] Therefore an equilibriumbetween the emulsification powder of the surfactant and theirconcentration is needed
35 Release Profile The release profile represents one of themost important characteristics of a nanomicro particulatecarrier system since their development has a main finalobjective the adequate release of the encapsulated bioactivemolecules to reach the desired clinical action
The release pattern of protein encapsulated in PLGAmicronanoparticles can present different behavior It ispossible to find a continuous release when the diffusionof the biomolecule is faster than the particle erosion Thisprocess involves a continuous diffusion of the protein fromthe polymer matrix before the PLGA particle is degradedin lactic and glycolic acid monomers by hydrolysis [74] Abiphasic release characterized by an initial burst at or nearthe particle surface followed by a second phase in whichprotein is progressively released by diffusion has also beendescribedThe second phase can be enhanced by bulk erosionof PLGA shell and matrix which results in an importantincrease of pores and channels [75] A third triphasic releaseprofile has been found when a lag release period occursafter initial burst and until polymer degradation starts [115]Finally it is possible to obtain an incomplete protein releaseas a consequence of additional factors related with theprotein-polymer interaction or protein instability Figure 6illustrates the different release profiles previously describedThe optimal carrier system should be capable of releasinga controlled concentration gradient of growth factors inthe appropriate time preventing or at least reducing orcontrolling the initial burst effect [116] A controlled initialburst followed by a sustained release significantly improvesthe in vivo bone regeneration [117ndash119]
Giteau et al [108] present an interesting revision on ldquoHowto achieve a sustained and complete release from PLGAmicroparticlesrdquo They begin by analyzing the influence of therelease medium and sampling method on the release profile
00
20
40
60
80
100
Cum
ulat
ive r
eleas
e of p
rote
in (
)
Time (h)500400300200100
Figure 6 Release profiles (I) BSA release from PLGA nanoparti-cles with high initial burst release (red dots line) biphasic modelcombining a moderate initial burst and a subsequent sustainedrelease (blue dash line) triphasicmodel with a lag of release betweenboth initial and sustained release phases (dash-dot green line)incomplete release
and highlight the significance of the centrifugation cleaningprocess or the releasemedium volume Adjusting to adequatevalues the centrifugation speed or the buffer volume itis possible to separate micronanoparticles from protein-containing release medium in a very easy wayThis allows forstable and reproducible release patterns On the other handto ensure a better protein release profile modification of themicroparticle formulation and microencapsulation processin order to preserve protein aggregation has to be performedProtein stability has to be maintained by preventing theformation of harmful medium For example the synthesisformulation can be modified to use more hydrophilic poly-mers since they have been shown to reduce the initial burstand to deliver bioactive proteins over long time periods
The most relevant strategies are referenced below Drugrelease from PLGA nanomicroparticles can be controlledby the polymer molecular weight and the relation betweenmonomers (lactideglycolide) so that an increase in gly-colic acid accelerates the weight loss of polymer due tothe higher hydrophilicity of the matrix [75] A mixtureof different PLGA nanoparticles obtained using 50 50 and75 50 latideglycolide ratio has shown a great potential forprotein drug delivery with a higher initial burst from PLGA50 50 A slow release period has been observed for PLGA75 50 encapsulating a glycoprotein (120572-1-antitrypsin) withclinic activity in some pulmonary diseases [60]
On the other hand a faster erosion of the microsphereswith reduction in the PLGA molecular weight due to thefacility of water penetration and the subsequent polymerdegradation has been described [83] Schrier et al workingwithmicrospheres prepared by wow using different types ofPLGA analyzed the important role of the molecular weightlactide-glycolide relation and acid residues [57]The amountof rhBMP2 adsorbed on the microparticle surface increased
10 BioMed Research International
with the hydrophobicity of the polymer At the same time therelease was in correlation with the degradation profile of thedifferent polymers [57]
Thus the use of more hydrophilic polymers reduces thehydrophobic protein-polymer interaction This effect favorsa more homogeneous distribution in the polymer matrixand increases the water uptake in the microspheres Thusthe release rate of rhBMP2 encapsulated in microspherescomposed by a PEG-PLGA di-block copolymer is increasedwith the PEG content of the polymer matrix [58] A similarresult was obtained using PLGA-PEG-PLGA triblock copoly-mers [59] In this case modifying the monomer relation(lactide-glycolide) in the PLGA and increasing the amountof PLGA-PEG-PLGA in the formulations the release profileof BMP-2 coencapsulated with human SA in microesphereswas adjustable Similarly the interaction of lysozyme withpoloxamer 188 before their encapsulation produces a sus-tained release over 3 weeks without any burst effect In thesame line using PLGA-PEG-PLGA as polymer a sustainedrelease of bioactive lysozime was extended over 45 days whenthe protein was complexed with poloxamer 188 previously tothe encapsulation [120] However the presence of PEG300 asan additive of the inner phase of microparticles during theencapsulation process also influences the protein distributionand the release profile In this case there is a decrease of theinitial burst but with less overall release [58]
On the other hand the use of PLGA-poloxamers blendsis useful to obtain a sustained release for more than onemonthwithout any incidence in the high initial burst [56 92]However for an encapsulated plasmid inside nanoparticlesobtained by PLGA-poloxamer blends the hydrophobicity ofthe surfactant allows prolonging the release up to 2 weeks in acontrolledmannerMoreover a complete release was reachedfor the PLGA-poloxamer blend instead PLGA nanoparticlesin which the maximum release was around 40 [84]
PLGA and poloxamers (pluronic F68) blends can also beused to obtain nanocomposite vesicles by a double emulsionprocess These vesicles are suitable for the encapsulation ofhydrophobic and hydrophilic molecules The presence ofpluronic affects the colloidal stability of the vesicles and therelease pattern of the encapsulated molecules These vesiclespresent a wall of 30 nm and the drug is encapsulated in thepresence of the poloxamer [121]
Other strategies include the use of different compounds toincrease the release timeThus BMP2 encapsulated in PLGA-PVA nanoparticles (around 300 nm) showed higher encapsu-lation efficiency and a short-time release profile with a veryhigh initial burst However with the same synthesis proce-dure (wow) but using PHBV (Poly(3 hydroxybutyrateco-3-hydroxivalerate)) BMP7 loaded nanocapsules had lessencapsulation efficiency despite a long-time delivery Nev-ertheless the maximum released amount was lower Thisdifference in the release profile was due to the differencein hydrophilicity and degradation rates of both polymers[122] Similarly PLGA-poloxamer blend nanoparticles weresuperficially modified by introducing chitosan in the secondstep of the synthesisThis method showed a sustained releaseprofile for up to 14 days without any initial important burstIn this case a recombinant hepatitis B antigen was used
[106] Moreover the use of heparin conjugated with PLGAporous microspheres has also been described to obtain along-time delivery system reducing at the same time theinitial burst In these systems heparin was immobilized ontothe nanomicroparticle surface The release was controlledby using the binding affinities of heparin to several growthfactors including BMP2 In this case the initial burst wasreduced to 4ndash7 during first day followed by a sustainedrelease of about 1 per day [51ndash53]
The initial burst release may be attenuated by thefabrication of double-wall microspheres that is core-shellmicroparticles The presence of a PLA shell reduces therelease rate of BSA encapsulated in the PLGA core andextends the duration of the release profile up to two monthsMoreover an increase in the PLA molecular weight influ-ences the rate of particle erosion which further slows theprotein release [123]
The modification of the viscosity in the environmentof microparticles additionally influences the release patternViscosity can control the burst at earliest time point andpromote a sustained release This situation has been shownfor rhBMP2-PLGA microspheres embedded in a chitosan-thioglycolic acid hydrogel (Poloxamer 407) [124] Yilgor etal also incorporated the nanoparticles of their sequentialdelivery system into a scaffold composed by chitosan andchitosan-PEO [54] In other work PLGAPVA microsphereswith encapsulated BMP2 were combined with differentcomposite biomaterials (gelatin hydrogel or polypropylenefumarate) The sustained release of the bioactive moleculewas extended over a period of 42 days In vivo results indicatethe importance of the composite characteristics In this casean enhanced bone formation was obtained when the PLGAmicroparticles were incorporated into the more hydrophobicmatrix (polypropylene fumarate) [125 126]
Finally Table 2 summarizes important information aboutdifferent parameters related to the use of PLGA basednano- ormicroparticles to encapsulate transport and releasegrowth factors (mainly BMP2)
36 Gene Therapy for Bone Tissue Engineering DirectedDelivery In the last years gene therapy has begun to playa role in bone tissue regeneration becoming an alternativemethod for the delivery of BMP2 [127 128] Thus the genesencoding a specific protein can be delivered to a specific cellrather than the proteins themselves To reach this purposean efficient gene vector is necessary Viral vectors possess thebest transfection efficiency but numerous disadvantages themost notable of them being the risk of mutagenesis Nonviralvectors elude these problems but with a significant reductionin the transfection rate [129]Therefore intracellular deliveryof bioactive agents has become the most used strategy forgene therapy looking for the adequate transfection andconsequent expression of the desired protein [79]
PLGA microspheres obtained by a wow double emul-sion process have been used by Qiao et al to entrap plasmid-BMP2polyethyleneimine nanoparticles In this case a sus-tained release of these nanoparticles until 35 days without ini-tial burst was found resulting in differentiation of osteoblast
BioMed Research International 11
Table 2 Nanomicroparticles systems to encapsulate GFs mainly BMP2 growth factor Most of them are in the microscopic scale andwere used to be entrapped into scaffold of different characteristics PVA has been the more used surfactant-stabilizer It is possible to findboth encapsulation and surface adsorption of the growth factors with high-moderate efficiency The use of heparin as stabilizer reducessignificantly the initial burst release favoring a sustained release in the time The bioactivity of the GF was preserved in most of the systemsand coencapsulation with other biomolecules seems to have a similar effect than the use of surfactants as stabilizers
Polymers Stabilizer Size Encapsulation EE Release Biological activity Reference
PLGA PVA 10ndash20120583m AdsorbedrhBMP2
20 ngmL ofconstant sustained
release
Better boneformation after 8
weeksFu et al 2013 [44]
PLGA PVA 10ndash100120583m rhBMP2-BSA69 (BMP)
Burst (20)Sustained until77 (28 days)
BMP2 moleculeswith bioactivity Tian et al 2012 [45]
PLGA 75 25 PVA 182120583m 82 mdash
Good bonedefect repair
outcomes within8minus12 weeks
Rodrıguez-Evoraet al 2014 [46]
PLGA PVA 228120583m 605
30 initial burstSlower release of4 per week After
8 weeks 60released
No loss ofbioactivity
Reyes et al 2013[47]
PLGAPEGNo doubleemulsionsynthesis
100ndash200 120583m Adsorbed BMP2
13 initial burstSlower release of001ndash8 per dayAfter 23 days 70
released
Substantial boneregeneration of the
scaffold
Rahman et al 2014[48]
Different PLGA PVA 20ndash100 120583m
30 (uncappedPLGA)
90 (cappedPLGA)
26ndash49 (1 day)Total after 2 weeks
No loss ofbioactivity
Lupu-Haber et al2013 [49]
PLGA 75 25 PVA 5ndash125 120583m mdashInitial burst 30 (1
day)Sustained 35 days
Higher volumesand surface areacoverage of new
bone
Wink et al 2014[50]
PLGA Heparin 200ndash800 nm Adsorbed BMP294
No initial burstSustained over 4
weeks
Significantreduction of theBMP2 dose forgood boneformation
La et al 2010 [51]
PLGA Heparin-Poloxamer 160 nm Adsorbed BMP2
100
Initial burst(4ndash7) linear
profile
Higher matrixmineralization ofregenerated bone
Chung et al 2007[52]
PLGA Heparin 100ndash250 nm Adsorbed 94Initial burst 10 (1
day)60 after 30 days
No loss ofbioactivityEfficacy of
administrationamount 50-fold
lower
Jeon et al 2008 [53]
PLGA PVA sim300 nm 80 85 initial burst (1day)
No loss ofbioactivity
Yilgor et al 2009[54]
PLGA (in rings) PVA 215 120583m 66Moderate burstSustained releaseover 6 weeks
60 of calvariadefect were healed
Rodrıguez-Evoraet al 2013 [55]
PLGA-Poloxamer 188Blend
Poloxamer 150 nmFGF-BSA-Heparin60ndash80
40 initial burst (1day) 60 (30 days)
No loss ofbioactivity
drsquoAngelo et al 2010[56]
Different PLGApolymers PVA 120583m order
rhBMP2adsorption40ndash75
20ndash80 initialburst (1 day) mdash Schrier et al 2001
[57]
12 BioMed Research International
Table 2 Continued
Polymers Stabilizer Size Encapsulation EE Release Biological activity Reference
PLGAPEG PVA 37ndash67 120583m 72ndash99 33 initial burst (1day)
Little loss ofbioactivity
Lochmann et al2010 [58]
PLGAPLGA-PEG-PLGA PVA 100 120583m HSA-BMP2
6070 initial burst (1
day)No loss ofbioactivity
White et al 2013[59]
PLGA PVA 100ndash1000 nm Α-1-antitrypsin90
30 initial burst (1day)
50 after 24 days
Biological activitywas preservedusing BSA and120573-cyclodextrine
Pirooznia et al2012 [60]
promoted by the correct transfection of the delivered bio-functional BMP2-DNA [130]
In spite of the general caution with gene therapy thegenetic delivery of BMP2 has the potentiality of a better safetycompared with the delivery of large amounts of recombinantprotein [131] Lu et al specify the urgent need to developmoreefficient delivery nanoparticles and transfection methods inorder to apply the nonviral vectors in stem cell engineeringand bone regeneration Although enhanced bone formationhas been shown in several recent studies using genes suchas HIF-1120572 and miRNAs new genetic sequences will bediscovered and used in bone engineering in the near futurethat will most likely change our perspective [132]
PLGA nanospheres represent a well-studied biomoleculedelivery system that could be applied to cell targeting inorder to enhance the delivery of specific proteins or nucleicacids inside or near the bone engineering reference cells thatis mesenchymal stem cells [133]The targeting properties canbe supplied by a ligand functionalization strategy modifica-tion of the surface structure of the nanocarrier by conjugatinga cell-specific ligand to direct the release of encapsulatedbiomolecules preferably in close association with the targetcells [134]The use of pegylated nanoparticles with a covalentattachment of different ligands is reported as a potentialtechnique to deliver bone cell-specific biomolecules for boneengineering [135]
Specific antibodies that recognize surface receptors inthese cells could be covalently coupled to the surface of PLGAnanoparticles obtaining ldquoimmunonanoparticlesrdquo There areseveral examples of antibody immobilization on surfaceof PLGA nanoparticles Kocbek et al demonstrated thespecific recognition of breast tumor cells by a specific mono-clonal antibody attached on PLGA fluorescent nanoparticlesobtained by WOW emulsion process [136] For the surfacecovalent attachment they used a more simple carbodiimidemethod which promotes the formation of an amide bondbetween free carboxylic end groups of PLGA nanoparticlesand primary amine groups of the antibody molecule [81]This procedure can be highly influenced by the presenceof stabilizers frequently used to confer colloidal stabilityto nanoparticles The electrophoretic mobility of PLGAnanoparticles with an antibody (immuno-120574-globuline anti-human C-reactive protein) covalently attached on the surfaceis shown in Figure 5 It is necessary to remark the drasticdecrease in the mobility values of the antibody-modified
nanoparticles with respect to bare PLGA nanoparticleswhich could imply low colloidal stability and the subse-quent aggregation of the nanosystem Santander-Ortega etal proposed a lower antibody loading in which the barePLGA patches must be coated by a nonionic surfactant inorder to obtain immunoreactive stable nanoparticles [95]Ratzinger et al indicated that the presence of high polox-amer concentrations decreased the coupling efficiency tocarboxylic end groups in PLGA nanoparticles showing thatan equilibrium that combines sufficient stability and the bestcoupling efficiency is necessary [98] To prevent this problemCheng et al synthetized carboxyl functionalized PLGA-PEG block copolymer attaching a specific aptamer to thesurface of pegylated nanoparticles via carbodiimide methodIn this work an enhanced drug delivery to prostate tumorshas been shown in comparison to equivalent nontargetednanoparticles [137]
37 Scaffolds The data reported in the literature indicatethat PLGA micronanoparticles are promising to achievea sustained spatial and temporally controlled delivery ofgrowth factors required for cell growth and cell differen-tiation They can be incorporated with cells in solid scaf-fold or injectable hydrogels [73] Scaffolds are porous 3Dstructures normally used to improve tissue-engineered bone[28] According to Tian et al [45] a scaffold designedwith this objective must have (1) appropriate mechanicalstrength to support the growth of new bone (2) appropriateporosity to allow ingrowth of bone-related cells (3) goodbiocompatibility allowing the growth of cells on its surfacewithout being rejected by the body and (4) low toxicity tocells and tissues surrounded and (5) must be able to induceosteogenic differentiation of bone-related stem cells and (6)be biodegradable with nontoxic degradation products thatcan be eventually replaced by new bone Additionally thescaffold for bone regeneration must maintain the delivery orrelease of BMP (growth factors) ldquoin siturdquo for a long time Inthis way nanomicroparticles inside scaffolds are being usedto release an adequate flow of these signaling biomoleculesand preserve their functional structure [138] The incorpo-ration of colloidal micronanoparticles into fibrous scaffoldsadds in the possibility of multiple drugs loading Howeverthis multidrug system could also involve a decrease ofthe mechanical properties of the structure and a possibleloss of nanoparticles entrapped between the fibers [139]
BioMed Research International 13
Considering that the in vivo half-life of most biomoleculesespecially proteins is relatively short it is essential thatbioactive scaffolds maintain a desired concentration ldquoin siturdquoto direct tissue regeneration To do so an initial release ofthe encapsulated growth factor in the first hours to quicklyget an effective therapeutic concentration followed by asustained long-time release profile is required [139] Most ofthe polymeric particles inserted in scaffold structures are ina micron-scale The main objective of these microparticlesis the protection and temporary control of growth factordelivery However given the porosity of these structuresnanoparticles and especially particles of a few microns maybecome more important since it is possible to design systemswith a simple and easy diffusion through the structure Thisprocess could allow the specific recognition of a particularcell type releasing their encapsulated BMPs in the sameenvironment and helping their differentiation to cellbonetissue In any case the larger-size microspheres might notnecessarily be useless for bone regeneration scaffolds As themicrospheres gradually degrade the space they occupied willbe conducive to ingrowth of tissue In addition to affectingthe compressionmodulus of scaffolds because of their hollowfeature the particle size of microspheres can also influencethe release of rhBMP2 [45]
4 Conclusion
The use of polymeric particles using PLGA is a promisingsystem for a spatially and temporally controlled delivery ofgrowth factors that promote cell growth and differentiationin bone engineering and regeneration by means of theirincorporation beside cells into solid scaffold or hydrogels
The PLGA is widely used for its biodegradability andbiocompatibility and is approved by FDA and the EuropeanMedicines Agency for use in drug delivery systems suppliedvia parenteral On the other hand BMPs are potent growthfactors for bone repair and specifically BMP2 shows excellentability to induce bone formation of adequate quality Theprocedure for synthesizing PLGA nano- or microparticlescan be modified in their different variables to obtain systemswith controlled size in which it is possible to encapsulatehydrophobic or hydrophilic molecules with an adequate col-loidal stability and the possibility of surface functionalizationfor targeted delivery
With this scenario an optimization of methods and com-ponentsmust balance the structure andmorphology of PLGAmicronanoparticles in order to achieve high encapsulationefficiency of BMP2 and looking for a main goal control ofdelivery reducing the initial burst and reaching a sustainedrelease profile preserving the biological activity and directedto the target cells tominimize the clinical amount needed andallowing a correct bone tissue regeneration
Conflict of Interests
The authors declare no conflict of interests with any of theproducts listed in the paper
Acknowledgments
The authors wish to express their appreciation for thefinancial support granted by the ldquoMinisterio de Educaciony Cienciardquo (MEC Spain) Projects MAT2013-43922-R andResearch Groups no FQM-115 no CTS-138 and no CTS-583 (Junta de Andalucıa Spain) Partial support was alsoprovided by the Andalucıa Talent Hub Program from theAndalusian Knowledge Agency cofunded by the EuropeanUnionrsquos Seventh Framework Program Marie Skłodowska-Curie actions (COFUND Grant Agreement no 291780) andthe Ministry of Economy Innovation Science and Employ-ment of the Junta de Andalucıa (Miguel Padial-Molina)
References
[1] M Padial-Molina P Galindo-Moreno and G Avila-OrtizldquoBiomimetic ceramics in implant dentistryrdquoMinerva Biotecno-logica vol 21 no 3 pp 173ndash186 2009
[2] B Al-Nawas and E Schiegnitz ldquoAugmentation proceduresusing bone substitute materials or autogenous bonemdasha sys-tematic review and meta-analysisrdquo European Journal of OralImplantology vol 7 supplement 2 pp S219ndashS234 2014
[3] A Katranji P Fotek and H-L Wang ldquoSinus augmentationcomplications etiology and treatmentrdquo Implant Dentistry vol17 no 3 pp 339ndash349 2008
[4] C E Misch ldquoMaxillary sinus augmentation for endostealimplants organized alternative treatment plansrdquo The Interna-tional Journal of Oral Implantology Implantologist vol 4 no 2pp 49ndash58 1987
[5] C Myeroff and M Archdeacon ldquoAutogenous bone graft donorsites and techniquesrdquo The Journal of Bone amp Joint SurgerymdashAmerican Volume vol 93 no 23 pp 2227ndash2236 2011
[6] G Avila R Neiva C E Misch et al ldquoClinical and histologicoutcomes after the use of a novel allograft for maxillary sinusaugmentation a case seriesrdquo Implant Dentistry vol 19 no 4pp 330ndash341 2010
[7] S J Froum S S Wallace N Elian S C Cho and D P TarnowldquoComparison of mineralized cancellous bone allograft (Puros)and anorganic bovine bonematrix (Bio-Oss) for sinus augmen-tation histomorphometry at 26 to 32 weeks after graftingrdquoTheInternational Journal of Periodontics amp Restorative Dentistryvol 26 no 6 pp 543ndash551 2006
[8] P Galindo-Moreno G Avila J E Fernandez-Barbero et alldquoEvaluation of sinus floor elevation using a composite bone graftmixturerdquo Clinical Oral Implants Research vol 18 no 3 pp 376ndash382 2007
[9] P Galindo-Moreno I Moreno-Riestra G Avila et al ldquoEffectof anorganic bovine bone to autogenous cortical bone ratioupon bone remodeling patterns following maxillary sinusaugmentationrdquo Clinical Oral Implants Research vol 22 no 8pp 857ndash864 2011
[10] S L Wheeler ldquoSinus augmentation for dental implants theuse of alloplastic materialsrdquo Journal of Oral and MaxillofacialSurgery vol 55 no 11 pp 1287ndash1293 1997
[11] S S Wallace and S J Froum ldquoEffect of maxillary sinusaugmentation on the survival of endosseous dental implantsA systematic reviewrdquo Annals of Periodontologythe AmericanAcademy of Periodontology vol 8 no 1 pp 328ndash343 2003
14 BioMed Research International
[12] M Padial-Molina and H F Rios ldquoStem cells scaffolds andgene therapy for periodontal engineeringrdquo Current Oral HealthReports vol 1 no 1 pp 16ndash25 2014
[13] M Padial-Molina S L Volk and H F Rios ldquoPeriostinincreases migration and proliferation of human periodontalligament fibroblasts challenged by tumor necrosis factor -alphaand Porphyromonas gingivalis lipopolysaccharidesrdquo Journal ofPeriodontal Research vol 49 no 3 pp 405ndash414 2014
[14] H Behnia A Khojasteh M Soleimani A Tehranchi and AAtashi ldquoRepair of alveolar cleft defect with mesenchymal stemcells and platelet derived growth factors a preliminary reportrdquoJournal of Cranio-Maxillofacial Surgery vol 40 no 1 pp 2ndash72012
[15] M Padial-Molina J T Marchesan A D Taut Q Jin WV Giannobile and H F Rios ldquoMethods to validate tooth-supporting regenerative therapiesrdquo Methods in Molecular Biol-ogy vol 887 pp 135ndash148 2012
[16] M R Urist ldquoBone formation by autoinductionrdquo Science vol150 no 3698 pp 893ndash899 1965
[17] P Boyne and S D Jones ldquoDemonstration of the osseoinductiveeffect of bone morphogenetic protein within endosseous dentalimplantsrdquo Implant Dentistry vol 13 no 2 pp 180ndash184 2004
[18] E A Wang V Rosen J S DrsquoAlessandro et al ldquoRecombinanthuman bone morphogenetic protein induces bone formationrdquoProceedings of the National Academy of Sciences of the UnitedStates of America vol 87 no 6 pp 2220ndash2224 1990
[19] J M Wozney ldquoThe bone morphogenetic protein family andosteogenesisrdquoMolecular Reproduction andDevelopment vol 32no 2 pp 160ndash167 1992
[20] E Barboza A Caula and F Machado ldquoPotential of recombi-nant human bone morphogenetic protein-2 in bone regenera-tionrdquo Implant Dentistry vol 8 no 4 pp 360ndash367 1999
[21] A C Carreira G G Alves W F Zambuzzi M C Sogayarand J M Granjeiro ldquoBone morphogenetic proteins structurebiological function and therapeutic applicationsrdquo Archives ofBiochemistry and Biophysics vol 561 pp 64ndash73 2014
[22] J C Bustos-Valenzuela A Fujita E Halcsik J M Granjeiroand M C Sogayar ldquoUnveiling novel genes upregulated by bothrhBMP2 and rhBMP7 during early osteoblastic transdifferen-tiation of C2C12 cellsrdquo BMC Research Notes vol 4 article 3702011
[23] K Tsuji A Bandyopadhyay B D Harfe et al ldquoBMP2 activityalthough dispensable for bone formation is required for theinitiation of fracture healingrdquo Nature Genetics vol 38 no 12pp 1424ndash1429 2006
[24] K Tsuji K Cox A Bandyopadhyay B D Harfe C J Tabin andV Rosen ldquoBMP4 is dispensable for skeletogenesis and fracture-healing in the limbrdquo The Journal of Bone and Joint SurgerymdashAmerican Volume vol 90 supplement 1 pp 14ndash18 2008
[25] K Tsuji K Cox L Gamer D Graf A Economides and VRosen ldquoConditional deletion of BMP7 from the limb skeletondoes not affect bone formation or fracture repairrdquo Journal ofOrthopaedic Research vol 28 no 3 pp 384ndash389 2010
[26] G Chen C Deng and Y-P Li ldquoTGF-beta and BMP signalingin osteoblast differentiation and bone formationrdquo InternationalJournal of Biological Sciences vol 8 no 2 pp 272ndash288 2012
[27] M-C Ramel and C S Hill ldquoSpatial regulation of BMP activityrdquoFEBS Letters vol 586 no 14 pp 1929ndash1941 2012
[28] A C Carreira F H Lojudice E Halcsik R D Navarro M CSogayar and J M Granjeiro ldquoBone morphogenetic proteinsfacts challenges and future perspectivesrdquo Journal of DentalResearch vol 93 no 4 pp 335ndash345 2014
[29] F Deschaseaux L Sensebe and D Heymann ldquoMechanisms ofbone repair and regenerationrdquo Trends in Molecular Medicinevol 15 no 9 pp 417ndash429 2009
[30] T DMueller and J Nickel ldquoPromiscuity and specificity in BMPreceptor activationrdquo FEBS Letters vol 586 no 14 pp 1846ndash1859 2012
[31] C Sieber J Kopf C Hiepen and P Knaus ldquoRecent advancesin BMP receptor signalingrdquo Cytokine amp Growth Factor Reviewsvol 20 no 5-6 pp 343ndash355 2009
[32] G Sapkota C Alarcon F M Spagnoli A H Brivanlou and JMassague ldquoBalancing BMP signaling through integrated inputsinto the Smad1 linkerrdquoMolecular Cell vol 25 no 3 pp 441ndash4542007
[33] W F McKay S M Peckham and J M Badura ldquoA comprehen-sive clinical review of recombinant human bonemorphogeneticprotein-2 (INFUSE Bone Graft)rdquo International Orthopaedicsvol 31 no 6 pp 729ndash734 2007
[34] P Hong D Boyd S D Beyea and M Bezuhly ldquoEnhancementof bone consolidation in mandibular distraction osteogenesisa contemporary review of experimental studies involving adju-vant therapiesrdquo Journal of Plastic Reconstructive and AestheticSurgery vol 66 no 7 pp 883ndash895 2013
[35] D B Spagnoli and R E Marx ldquoDental implants and the use ofrhBMP-2rdquo Dental Clinics of North America vol 55 no 4 pp883ndash907 2011
[36] M Nevins C Kirker-HeadM Nevins J AWozney R Palmerand D Graham ldquoBone formation in the goat maxillary sinusinduced by absorbable collagen sponge implants impregnatedwith recombinant human bone morphogenetic protein-2rdquo TheInternational Journal of Periodontics amp Restorative Dentistryvol 16 no 1 pp 8ndash19 1996
[37] P J Boyne L C Lilly R E Marx et al ldquoDe novo bone induc-tion by recombinant human bone morphogenetic protein-2(rhBMP-2) in maxillary sinus floor augmentationrdquo Journal ofOral and Maxillofacial Surgery vol 63 no 12 pp 1693ndash17072005
[38] L Torrecillas-Martinez A Monje M A Pikos et al ldquoEffect ofrhBMP-2 uponmaxillary sinus augmentation a comprehensivereviewrdquo Implant Dentistry vol 22 no 3 pp 232ndash237 2013
[39] J Lee C Susin N A Rodriguez et al ldquoSinus augmentationusing rhBMP-2ACS in a mini-pig model relative efficacy ofautogenous fresh particulate iliac bone graftsrdquo Clinical OralImplants Research vol 24 no 5 pp 497ndash504 2013
[40] RG TriplettMNevins R EMarx et al ldquoPivotal randomizedparallel evaluation of recombinant human bonemorphogeneticprotein-2absorbable collagen sponge and autogenous bonegraft for maxillary sinus floor augmentationrdquo Journal of Oraland Maxillofacial Surgery vol 67 no 9 pp 1947ndash1960 2009
[41] D W K Kao A Kubota M Nevins and J P Fiorellini ldquoThenegative effect of combining rhBMP-2 and Bio-Oss on boneformation for maxillary sinus augmentationrdquoThe InternationalJournal of Periodontics amp Restorative Dentistry vol 32 no 1 pp61ndash67 2012
[42] O Hanisch D N Tatakis M D Rohrer P S Wohrle JM Wozney and U M E Wikesjo ldquoBone formation andosseointegration stimulated by rhBMP-2 following subantralaugmentation procedures in nonhuman primatesrdquoThe Interna-tional Journal of Oral ampMaxillofacial Implants vol 12 no 6 pp785ndash792 1997
[43] K Wada A Niimi K Watanabe T Sawai and M UedaldquoMaxillary sinus floor augmentation in rabbits a comparative
BioMed Research International 15
histologic-histomorphometric study between rhBMP-2 andautogenous bonerdquo The International Journal of Periodontics ampRestorative Dentistry vol 21 no 3 pp 253ndash263 2001
[44] R Fu S Selph M McDonagh et al ldquoEffectiveness and harmsof recombinant human bone morphogenetic protein-2 in spinefusion a systematic review and meta-analysisrdquo Annals of Inter-nal Medicine vol 158 no 12 pp 890ndash902 2013
[45] Z Tian Y Zhu J Qiu et al ldquoSynthesis and characterization ofUPPE-PLGA-rhBMP2 scaffolds for bone regenerationrdquo Journalof Huazhong University of Science and TechnologymdashMedicalScience vol 32 no 4 pp 563ndash570 2012
[46] M Rodrıguez-Evora E Garcıa-Pizarro C del Rosario et alldquoSmurf1 knocked-down mesenchymal stem cells and BMP-2 in an electrospun system for bone regenerationrdquo Biomacro-molecules vol 15 no 4 pp 1311ndash1322 2014
[47] R Reyes A Delgado R Solis et al ldquoCartilage repair bylocal delivery of TGF-1205731 or BMP-2 from a novel segmentedpolyurethanepolylactic-co-glycolic bilayered scaffoldrdquo Journalof Biomedical Materials Research Part A 2013
[48] C V Rahman D Ben-David A Dhillon et al ldquoControlledrelease of BMP-2 from a sintered polymer scaffold enhancesbone repair in a mouse calvarial defect modelrdquo Journal of TissueEngineering and Regenerative Medicine vol 8 no 1 pp 59ndash662014
[49] Y Lupu-Haber O Pinkas S Boehm T Scheper C Kasper andM Machluf ldquoFunctionalized PLGA-doped zirconium oxideceramics for bone tissue regenerationrdquoBiomedicalMicrodevicesvol 15 no 6 pp 1055ndash1066 2013
[50] J D Wink P A Gerety R D Sherif et al ldquoSustaineddelivery of rhBMP-2 by means of poly(lactic-co-glycolic acid)microspheres cranial bone regeneration without heterotopicossification or craniosynostosisrdquo Plastic and ReconstructiveSurgery vol 134 no 1 pp 51ndash59 2014
[51] W-G La S-W Kang H S Yang et al ldquoThe efficacy of bonemorphogenetic protein-2 depends on its mode of deliveryrdquoArtificial Organs vol 34 no 12 pp 1150ndash1153 2010
[52] Y-I Chung K-M Ahn S-H Jeon S-Y Lee J-H Lee and GTae ldquoEnhanced bone regeneration with BMP-2 loaded func-tional nanoparticle-hydrogel complexrdquo Journal of ControlledRelease vol 121 no 1-2 pp 91ndash99 2007
[53] O Jeon S J Song H S Yang et al ldquoLong-term deliveryenhances in vivo osteogenic efficacy of bone morphogeneticprotein-2 compared to short-term deliveryrdquo Biochemical andBiophysical Research Communications vol 369 no 2 pp 774ndash780 2008
[54] P Yilgor K Tuzlakoglu R L Reis N Hasirci and V HasircildquoIncorporation of a sequential BMP-2BMP-7 delivery systeminto chitosan-based scaffolds for bone tissue engineeringrdquoBiomaterials vol 30 no 21 pp 3551ndash3559 2009
[55] M Rodrıguez-Evora A Delgado R Reyes et al ldquoOsteogeniceffect of local long versus short term BMP-2 delivery froma novel SPU-PLGA-120573TCP concentric system in a critical sizedefect in ratsrdquo European Journal of Pharmaceutical Sciences vol49 no 5 pp 873ndash884 2013
[56] I drsquoAngelo M Garcia-Fuentes Y Parajo et al ldquoNanoparticlesbased on PLGA poloxamer blends for the delivery of proangio-genic growth factorsrdquoMolecular Pharmaceutics vol 7 no 5 pp1724ndash1733 2010
[57] J A Schrier B F Fink J B Rodgers H C Vasconez and PP DeLuca ldquoEffect of a freeze-dried CMCPLGA microspherematrix of rhBMP-2 on bone healingrdquo AAPS PharmSciTech vol2 no 3 article E18 2001
[58] A Lochmann H Nitzsche S von Einem E Schwarz and KMader ldquoThe influence of covalently linked and free polyethy-lene glycol on the structural and release properties of rhBMP-2loaded microspheresrdquo Journal of Controlled Release vol 147 no1 pp 92ndash100 2010
[59] L J White G T S Kirby H C Cox et al ldquoAcceleratingprotein release from microparticles for regenerative medicineapplicationsrdquoMaterials Science and Engineering C Materials forBiological Applications vol 33 no 5 pp 2578ndash2583 2013
[60] N Pirooznia S Hasannia A S Lotfi and M Ghanei ldquoEncap-sulation of alpha-1 antitrypsin in PLGA nanoparticles in vitrocharacterization as an effective aerosol formulation in pul-monary diseasesrdquo Journal of Nanobiotechnology vol 10 article20 2012
[61] M Ronga A Fagetti G Canton E Paiusco M F Surace andP Cherubino ldquoClinical applications of growth factors in boneinjuries experience with BMPsrdquo Injury vol 44 supplement 1pp S34ndashS39 2013
[62] J G Devine J R Dettori J C France E Brodt and R AMcGuire ldquoThe use of rhBMP in spine surgery is there a cancerriskrdquo Evidence-Based Spine-Care Journal vol 3 no 2 pp 35ndash41 2012
[63] E J Carragee E L Hurwitz and B KWeiner ldquoA critical reviewof recombinant human bone morphogenetic protein-2 trials inspinal surgery emerging safety concerns and lessons learnedrdquoThe Spine Journal vol 11 no 6 pp 471ndash491 2011
[64] M C Simmonds J V E Brown M K Heirs et al ldquoSafetyand effectiveness of recombinant human bone morphogeneticprotein-2 for spinal fusion a meta-analysis of individual-participant datardquo Annals of Internal Medicine vol 158 no 12pp 877ndash889 2013
[65] V H-Y Chung A Y-L Chen L-B Jeng C-C Kwan S-H Cheng and S C-N Chang ldquoEngineered autologous bonemarrow mesenchymal stem cells alternative to cleft alveolarbone graft surgeryrdquo Journal of Craniofacial Surgery vol 23 no5 pp 1558ndash1563 2012
[66] T A Ratko S E Belinson D J Samson C Bonnell K MZiegler and N Aronson BoneMorphogenetic ProteinThe Stateof the Evidence of On-Label and Off-Label Use Agency forHealthcare Research and Quality Rockville Md USA 2010
[67] G Barratt ldquoColloidal drug carriers achievements and perspec-tivesrdquo Cellular and Molecular Life Sciences vol 60 no 1 pp 21ndash37 2003
[68] V W Bramwell and Y Perrie ldquoParticulate delivery systems forvaccinesrdquo Critical Reviews inTherapeutic Drug Carrier Systemsvol 22 no 2 pp 151ndash214 2005
[69] N Csaba M Garcia-Fuentes and M J Alonso ldquoThe perfor-mance of nanocarriers for transmucosal drug deliveryrdquo ExpertOpinion on Drug Delivery vol 3 no 4 pp 463ndash478 2006
[70] M J Santander-Ortega T Stauner B Loretz et al ldquoNanopar-ticles made from novel starch derivatives for transdermal drugdeliveryrdquo Journal of Controlled Release vol 141 no 1 pp 85ndash922010
[71] W Jiang R K Gupta M C Deshpande and S P Schwende-man ldquoBiodegradable poly(lactic-co-glycolic acid) microparti-cles for injectable delivery of vaccine antigensrdquo Advanced DrugDelivery Reviews vol 57 no 3 pp 391ndash410 2005
[72] T R Shantha Kumar K Soppimath and S K NachaegarildquoNovel delivery technologies for protein and peptide therapeu-ticsrdquo Current Pharmaceutical Biotechnology vol 7 no 4 pp261ndash276 2006
16 BioMed Research International
[73] F Danhier E Ansorena J M Silva R Coco A Le Bretonand V Preat ldquoPLGA-based nanoparticles an overview ofbiomedical applicationsrdquo Journal of Controlled Release vol 161no 2 pp 505ndash522 2012
[74] A Kumari S K Yadav and S C Yadav ldquoBiodegradablepolymeric nanoparticles based drug delivery systemsrdquo Colloidsand Surfaces B Biointerfaces vol 75 no 1 pp 1ndash18 2010
[75] H K Makadia and S J Siegel ldquoPoly Lactic-co-Glycolic Acid(PLGA) as biodegradable controlled drug delivery carrierrdquoPolymers vol 3 no 3 pp 1377ndash1397 2011
[76] F Mohamed and C F van der Walle ldquoEngineering biodegrad-able polyester particles with specific drug targeting and drugrelease propertiesrdquo Journal of Pharmaceutical Sciences vol 97no 1 pp 71ndash87 2008
[77] G A Silva O P Coutinho P Ducheyne andR L Reis ldquoMateri-als in particulate form for tissue engineering 2 Applications inbonerdquo Journal of Tissue Engineering and Regenerative Medicinevol 1 no 2 pp 97ndash109 2007
[78] S Zhang and H Uludag ldquoNanoparticulate systems for growthfactor deliveryrdquoPharmaceutical Research vol 26 no 7 pp 1561ndash1580 2009
[79] V E Santo M E Gomes J F Mano and R L Reis ldquoFromnano-to macro-scale nanotechnology approaches for spatiallycontrolled delivery of bioactive factors for bone and cartilageengineeringrdquo Nanomedicine vol 7 no 7 pp 1045ndash1066 2012
[80] M-K Tran A Swed and F Boury ldquoPreparation of polymericparticles in CO
2medium using non-toxic solvents formulation
and comparisons with a phase separation methodrdquo EuropeanJournal of Pharmaceutics and Biopharmaceutics vol 82 no 3pp 498ndash507 2012
[81] B Ertl F Heigl M Wirth and F Gabor ldquoLectin-mediatedbioadhesion preparation stability andCaco-2 binding of wheatgerm agglutinin-functionalized poly-(DL-lactic-co-glycolicacid)-microspheresrdquo Journal of Drug Targeting vol 8 no 3 pp173ndash184 2000
[82] M L Hans and A M Lowman ldquoBiodegradable nanoparticlesfor drug delivery and targetingrdquo Current Opinion in Solid Stateand Materials Science vol 6 no 4 pp 319ndash327 2002
[83] D Blanco and M J Alonso ldquoProtein encapsulation and releasefrom poly(lactide-co-glycolide) microspheres effect of the pro-tein and polymer properties and of the co-encapsulation ofsurfactantsrdquo European Journal of Pharmaceutics and Biophar-maceutics vol 45 no 3 pp 285ndash294 1998
[84] N Csaba P Caamano A Sanchez F Domınguez and MJ Alonso ldquoPLGA poloxamer and PLGApoloxamine blendnanoparticles new carriers for gene deliveryrdquo Biomacro-molecules vol 6 no 1 pp 271ndash278 2005
[85] C Sturesson and J Carlfors ldquoIncorporation of protein inPLG-microspheres with retention of bioactivityrdquo Journal ofControlled Release vol 67 no 2-3 pp 171ndash178 2000
[86] I D Rosca F Watari and M Uo ldquoMicroparticle formationand its mechanism in single and double emulsion solventevaporationrdquo Journal of Controlled Release vol 99 no 2 pp271ndash280 2004
[87] F T Meng G H Ma W Qiu and Z G Su ldquoWOW doubleemulsion technique using ethyl acetate as organic solventeffects of its diffusion rate on the characteristics of micropar-ticlesrdquo Journal of Controlled Release vol 91 no 3 pp 407ndash4162003
[88] R Ghaderi and J Carlfors ldquoBiological activity of lysozyme afterentrapment in poly (dl-lactide-co-glycolide)-microspheresrdquoPharmaceutical Research vol 14 no 11 pp 1556ndash1562 1997
[89] H Wang S C G Leeuwenburgh Y Li and J A Jansen ldquoTheuse of micro- and nanospheres as functional components forbone tissue regenerationrdquo Tissue Engineering Part B Reviewsvol 18 no 1 pp 24ndash39 2012
[90] S Xiong X Zhao B C Heng K W Ng and J S-C LooldquoCellular uptake of Poly-(DL-lactide-co-glycolide) (PLGA)nanoparticles synthesized through solvent emulsion evapora-tion and nanoprecipitation methodrdquo Biotechnology Journal vol6 no 5 pp 501ndash508 2011
[91] L Y T Chou K Ming and W C W Chan ldquoStrategies forthe intracellular delivery of nanoparticlesrdquo Chemical SocietyReviews vol 40 no 1 pp 233ndash245 2011
[92] M J Santander-Ortega M V Lozano-Lopez D Bastos-Gonzalez J M Peula-Garcıa and J L Ortega-Vinuesa ldquoNovelcore-shell lipid-chitosan and lipid-poloxamer nanocapsulesstability by hydration forcesrdquo Colloid and Polymer Science vol288 no 2 pp 159ndash172 2010
[93] Y-Y Yang T-S Chung and N Ping Ng ldquoMorphology drugdistribution and in vitro release profiles of biodegradable poly-meric microspheres containing protein fabricated by double-emulsion solvent extractionevaporation methodrdquo Biomateri-als vol 22 no 3 pp 231ndash241 2001
[94] T Feczko J Toth and J Gyenis ldquoComparison of the prepara-tion of PLGA-BSA nano- and microparticles by PVA polox-amer and PVPrdquo Colloids and Surfaces A Physicochemical andEngineering Aspects vol 319 no 1ndash3 pp 188ndash195 2008
[95] M J Santander-Ortega D Bastos-Gonzalez and J L Ortega-Vinuesa ldquoElectrophoretic mobility and colloidal stability ofPLGA particles coated with IgGrdquo Colloids and Surfaces BBiointerfaces vol 60 no 1 pp 80ndash88 2007
[96] Y-Y Yang H-H Chia and T-S Chung ldquoEffect of prepara-tion temperature on the characteristics and release profiles ofPLGA microspheres containing protein fabricated by double-emulsion solvent extractionevaporation methodrdquo Journal ofControlled Release vol 69 no 1 pp 81ndash96 2000
[97] D-L Fang Y Chen B Xu et al ldquoDevelopment of lipid-shell andpolymer core nanoparticles with water-soluble salidroside foranti-cancer therapyrdquo International Journal ofMolecular Sciencesvol 15 no 3 pp 3373ndash3388 2014
[98] G Ratzinger U Langer L Neutsch F Pittner M Wirth and FGabor ldquoSurface modification of PLGA particles the interplaybetween stabilizer ligand size and hydrophobic interactionsrdquoLangmuir vol 26 no 3 pp 1855ndash1859 2010
[99] S-S Feng and G Huang ldquoEffects of emulsifiers on thecontrolled release of paclitaxel (Taxol) from nanospheres ofbiodegradable polymersrdquo Journal of Controlled Release vol 71no 1 pp 53ndash69 2001
[100] JMChan L ZhangK P Yuet et al ldquoPLGA-lecithin-PEGcore-shell nanoparticles for controlled drug deliveryrdquo Biomaterialsvol 30 no 8 pp 1627ndash1634 2009
[101] M Fraylich W Wang K Shakesheff C Alexander andB Saunders ldquoPoly(DL-lactide-co-glycolide) dispersions con-taining pluronics from particle preparation to temperature-triggered aggregationrdquo Langmuir vol 24 no 15 pp 7761ndash77682008
[102] M J Santander-Ortega J M Peula-Garcıa F M Goycooleaand J L Ortega-Vinuesa ldquoChitosan nanocapsules effect ofchitosan molecular weight and acetylation degree on electroki-netic behaviour and colloidal stabilityrdquo Colloids and Surfaces BBiointerfaces vol 82 no 2 pp 571ndash580 2011
[103] J S Tan D E Butterfield C L Voycheck K D Caldwell and JT Li ldquoSurfacemodification of nanoparticles by PEOPPOblock
BioMed Research International 17
copolymers to minimize interactions with blood componentsand prolong blood circulation in ratsrdquo Biomaterials vol 14 no11 pp 823ndash833 1993
[104] C Bouissou U Potter H Altroff H Mardon and C van derWalle ldquoControlled release of the fibronectin central cell bindingdomain from polymeric microspheresrdquo Journal of ControlledRelease vol 95 no 3 pp 557ndash566 2004
[105] R Gref Y Minamitake M T Peracchia V Trubetskoy VTorchilin and R Langer ldquoBiodegradable long-circulating poly-meric nanospheresrdquo Science vol 263 no 5153 pp 1600ndash16031994
[106] P Paolicelli C Prego A Sanchez and M J Alonso ldquoSurface-modified PLGA-based nanoparticles that can efficiently asso-ciate and deliver virus-like particlesrdquo Nanomedicine vol 5 no6 pp 843ndash853 2010
[107] I Brigger C Dubernet and P Couvreur ldquoNanoparticles incancer therapy and diagnosisrdquoAdvancedDrugDelivery Reviewsvol 54 no 5 pp 631ndash651 2002
[108] A Giteau M C Venier-Julienne A Aubert-Pouessel and J PBenoit ldquoHow to achieve sustained and complete protein releasefrom PLGA-based microparticlesrdquo International Journal ofPharmaceutics vol 350 no 1-2 pp 14ndash26 2008
[109] E R Balmayor G A Feichtinger H S Azevedo Mvan Griensven and R L Reis ldquoStarch-poly-120576-caprolactonemicroparticles reduce the needed amount of BMP-2rdquo ClinicalOrthopaedics and Related Research vol 467 no 12 pp 3138ndash3148 2009
[110] M J Santander-Ortega D Bastos-Gonzalez J L Ortega-Vinuesa andM J Alonso ldquoInsulin-loaded PLGA nanoparticlesfor oral administration an in vitro physico-chemical character-izationrdquo Journal of Biomedical Nanotechnology vol 5 no 1 pp45ndash53 2009
[111] L Meinel O E Illi J Zapf M Malfanti H Peter Merkleand B Gander ldquoStabilizing insulin-like growth factor-I inpoly(DL-lactide-co-glycolide) microspheresrdquo Journal of Con-trolled Release vol 70 no 1-2 pp 193ndash202 2001
[112] C Srinivasan Y K Katare T Muthukumaran and A K PandaldquoEffect of additives on encapsulation efficiency stability andbioactivity of entrapped lysozyme from biodegradable polymerparticlesrdquo Journal of Microencapsulation vol 22 no 2 pp 127ndash138 2005
[113] M Tobıo S P Schwendeman Y Guo J McIver R Langerand M J Alonso ldquoImproved immunogenicity of a core-coatedtetanus toxoid delivery systemrdquoVaccine vol 18 no 7-8 pp 618ndash622 1999
[114] D K Malik S Baboota A Ahuja S Hasan and J Ali ldquoRecentadvances in protein and peptide drug delivery systemsrdquoCurrentDrug Delivery vol 4 no 2 pp 141ndash151 2007
[115] J L Cleland ldquoProtein delivery from biodegradable micro-spheresrdquo in Protein Delivery Physical Systems L M Sandersand R W Hendron Eds pp 1ndash41 Plenum Press New YorkNY USA 1997
[116] S H Oh T H Kim and J H Lee ldquoCreating growth factorgradients in three dimensional porous matrix by centrifugationand surface immobilizationrdquo Biomaterials vol 32 no 32 pp8254ndash8260 2011
[117] B N Brown J E Valentin A M Stewart-Akers G P McCabeand S F Badylak ldquoMacrophage phenotype and remodelingoutcomes in response to biologic scaffolds with and without acellular componentrdquo Biomaterials vol 30 no 8 pp 1482ndash14912009
[118] B N Brown J M Freund L Han et al ldquoComparison of threemethods for the derivation of a biologic scaffold composed ofadipose tissue extracellularmatrixrdquoTissue EngineeringmdashPart CMethods vol 17 no 4 pp 411ndash421 2011
[119] B Li T Yoshii A E Hafeman J S Nyman J C Wenkeand S A Guelcher ldquoThe effects of rhBMP-2 released frombiodegradable polyurethanemicrosphere composite scaffoldson new bone formation in rat femorardquo Biomaterials vol 30 no35 pp 6768ndash6779 2009
[120] A Paillard-Giteau V T Tran O Thomas et al ldquoEffect ofvarious additives and polymers on lysozyme release fromPLGAmicrospheres prepared by an sow emulsion techniquerdquoEuropean Journal of Pharmaceutics and Biopharmaceutics vol75 no 2 pp 128ndash136 2010
[121] B P Nair and C P Sharma ldquoPoly(lactide-co-glycolide)-laponite-F68 nanocomposite vesicles through a single-stepdouble-emulsion method for the controlled release of doxoru-bicinrdquo Langmuir vol 28 no 9 pp 4559ndash4564 2012
[122] P Yilgor N Hasirci and V Hasirci ldquoSequential BMP-2BMP-7 delivery from polyester nanocapsulesrdquo Journal of BiomedicalMaterials ResearchmdashPart A vol 93 no 2 pp 528ndash536 2010
[123] Y Xia Q Xu C-H Wang and D W Pack ldquoProtein encapsu-lation in and release from monodisperse double-wall polymermicrospheresrdquo Journal of Pharmaceutical Sciences vol 102 no5 pp 1601ndash1609 2013
[124] Y Fu L Du QWang et al ldquoIn vitro sustained release of recom-binant human bone morphogenetic protein-2 microspheresembedded in thermosensitive hydrogelsrdquo Die Pharmazie vol67 no 4 pp 299ndash303 2012
[125] D H R Kempen L Lu T E Hefferan et al ldquoRetention ofin vitro and in vivo BMP-2 bioactivities in sustained deliveryvehicles for bone tissue engineeringrdquo Biomaterials vol 29 no22 pp 3245ndash3252 2008
[126] D H R Kempen L Lu A Heijink et al ldquoEffect of localsequential VEGF andBMP-2 delivery on ectopic and orthotopicbone regenerationrdquo Biomaterials vol 30 no 14 pp 2816ndash28252009
[127] H Nie M-L Ho C-K Wang C-H Wang and Y-C FuldquoBMP-2 plasmid loaded PLGAHAp composite scaffolds fortreatment of bone defects in nude micerdquo Biomaterials vol 30no 5 pp 892ndash901 2009
[128] F Wegman Y van der Helm F C Oner W J A Dhert andJ Alblas ldquoBone morphogenetic protein-2 plasmid DNA as asubstitute for bone morphogenetic protein-2 protein in bonetissue engineeringrdquo Tissue Engineering Part A vol 19 no 23-24pp 2686ndash2692 2013
[129] J Fischer A Kolk S Wolfart et al ldquoFuture of local boneregenerationmdashprotein versus gene therapyrdquo Journal of Cranio-Maxillofacial Surgery vol 39 no 1 pp 54ndash64 2011
[130] C Qiao K Zhang H Jin et al ldquoUsing poly(lactic-co-glycolicacid) microspheres to encapsulate plasmid of bone morpho-genetic protein 2polyethylenimine nanoparticles to promotebone formation in vitro and in vivordquo International Journal ofNanomedicine vol 8 pp 2985ndash2995 2013
[131] C H Evans ldquoGene delivery to bonerdquo Advanced Drug DeliveryReviews vol 64 no 12 pp 1331ndash1340 2012
[132] C-H Lu Y-H Chang S-Y Lin K-C Li andY-CHu ldquoRecentprogresses in gene delivery-based bone tissue engineeringrdquoBiotechnology Advances vol 31 no 8 pp 1695ndash1706 2013
[133] TNVo F K Kasper andAGMikos ldquoStrategies for controlleddelivery of growth factors and cells for bone regenerationrdquo
18 BioMed Research International
Advanced Drug Delivery Reviews vol 64 no 12 pp 1292ndash13092012
[134] W Ji H Wang J J J P van den Beucken et al ldquoLocal deliveryof small and large biomolecules in craniomaxillofacial bonerdquoAdvanced Drug Delivery Reviews vol 64 no 12 pp 1152ndash11642012
[135] V Luginbuehl L Meinel H P Merkle and B Gander ldquoLocal-ized delivery of growth factors for bone repairrdquo EuropeanJournal of Pharmaceutics and Biopharmaceutics vol 58 no 2pp 197ndash208 2004
[136] P Kocbek N Obermajer M Cegnar J Kos and J Kristl ldquoTar-geting cancer cells using PLGA nanoparticles surface modifiedwith monoclonal antibodyrdquo Journal of Controlled Release vol120 no 1-2 pp 18ndash26 2007
[137] J Cheng B A Teply I Sherifi et al ldquoFormulation of func-tionalized PLGA-PEG nanoparticles for in vivo targeted drugdeliveryrdquo Biomaterials vol 28 no 5 pp 869ndash876 2007
[138] C Romagnoli F DrsquoAsta andM L Brandi ldquoDrug delivery usingcomposite scaffolds in the context of bone tissue engineeringrdquoClinical Cases inMineral and BoneMetabolism vol 10 no 3 pp155ndash161 2013
[139] D Puppi X Zhang L Yang F Chiellini X Sun and EChiellini ldquoNanomicrofibrous polymeric constructs loadedwith bioactive agents and designed for tissue engineeringapplications a reviewrdquo Journal of Biomedical Materials ResearchPart B Applied Biomaterials vol 102 no 7 pp 1562ndash1579 2014
Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595
Contents lists available at ScienceDirect
Colloids and Surfaces B Biointerfaces
jo ur nal ho me p ag e wwwelsev ier com locate co lsur fb
Full Length Article
Dual delivery nanosystem for biomolecules Formulationcharacterization and in vitro release
Inmaculada Ortega-Oller a1 Teresa del Castillo-Santaella b1 Miguel Padial-Molina aPablo Galindo-Moreno a Ana Beleacuten Joacutedar-Reyes b Joseacute Manuel Peula-Garciacutea bclowast
a Department of Oral Surgery and Implant Dentistry University of Granada Granada Spainb Biocolloid and Fluid Physics Group Department of Applied Physics University of Granada 18071 Granada Spainc Department of Applied Physics II University of Malaga 29071 Malaga Spain
a r t i c l e i n f o
Article historyReceived 25 May 2017Received in revised form 18 July 2017Accepted 17 August 2017
KeywordsPLGANanoparticlesProtein encapsulationRelease
a b s t r a c t
Because of the biocompatible and biodegradable properties of poly (lactic-co-glycolic acid) (PLGA)nanoparticles (NPs) based on this polymer have been widely studied for drugbiomolecule delivery andlong-term sustained-release In this work two different formulation methods for lysozyme-loaded PLGANPs have been developed and optimized based on the double-emulsion (wateroilwater WOW) sol-vent evaporation technique They differ mainly in the phase in which the surfactant (Pluronicreg F68) isadded water (W-F68) and oil (O-F68) The colloidal properties of these systems (morphology by SEM andSTEM hydrodynamic size by DLS and NTA electrophoretic mobility temporal stability in different mediaprotein encapsulation release and bioactivity) have been analyzed The interaction surfactant-proteindepending on the formulation procedure has been characterized by surface tension and dilatational rhe-ology Finally cellular uptake by human mesenchymal stromal cells and cytotoxicity for both systemshave been analyzed
Spherical hard NPs are made by the two methods However in one case they are monodisperse withdiameters of around 120 nm (O-F68) and in the other case a polydisperse system of NPs with diametersbetween 100 and 500 nm is found (W-F68) Protein encapsulation efficiency release and bioactivity aremaintained better by the W-F68 formulation method This multimodal system is found to be a promisingldquodual deliveryrdquo system for encapsulating hydrophilic proteins with strong biological activity at the cell-surface and cytoplasmic levels
copy 2017 Elsevier BV All rights reserved
1 Introduction
Tissue regeneration is a complex biological action involvingmultiple steps in a sequential ordered and controlled manner [12]Classically bioactive molecules have been proposed to aid in theseprocesses However the use of high doses denaturation and lossof biological activity uncontrolled timing of action and diffusionto other tissues have been highlighted as major issues of this ther-apeutic strategy [3] To help solve these problems nanomedicinehas been intensively investigated in recent years as an emerging
lowast Corresponding author at Department of Applied Physics II University of Maacutelaga29071 Maacutelaga Spain
E-mail address jmpeulaumaes (JM Peula-Garciacutea)1 Both authors contributed equally to this work
area This involves diagnostic therapeutic and regeneration meth-ods by means of structures and systems in which size and shape arecontrolled at the atomic molecular and supramolecular levels [4]The transport and controlled delivery of drugs andor therapeuticbiomolecules improve their pharmacokinetics and pharmacody-namics and at the same time minimize harmful side effects Forthese purposes different nanosystems have been described Polylactic-co-glycolic acid (PLGA) exhibits low cytotoxicity as well ashigh biocompatibility and biodegradability with the release of non-toxic by-products [5]
In the last decade the use of PLGA has been investigated todeliver a wide spectrum of active agents from hydrophobic drugmolecules [6ndash8] to hydrophilic biomolecules as peptides [9] pro-teins [10ndash15] or nucleic acids [1617] These delivery systemshave been produced via different formulation processes for theirapplication in both systemic and local site-specific therapies [18]
httpdxdoiorg101016jcolsurfb2017080270927-7765copy 2017 Elsevier BV All rights reserved
I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595 587
However their design and development as nanocarriers are diffi-cult due to the problematic release pattern when the encapsulatedmolecules are proteins for which initial bursts and slow or incom-plete release might be a problem [18ndash20] Moreover the specificconditions of the release may need to be different depending onthe final application of the nanocarrier [2021]
The water-in-oil-in-water (WOW) double emulsion techniqueis the most widely used protein-encapsulation method for PLGAmicro- (MP) and nanoparticles (NP) [2223] It allows differentfactors to be modulated such as the type of PLGA the use ofother polymers blended with PLGA the addition of surfactants themechanical stress or the organic solvent [20] It is also possible toconstruct several types of co-polymers to modify the hydropho-bicityhydrophilicity ratio [1824] and the colloidal stability sizeand release process PLGApolyethylene glycol pair and surfactantssuch as polyvinyl alcohol (PVA) or polyethylene oxides (PEO) arethe most widely studied [7122526]
On the other hand tissue engineering requires the participa-tion of mesenchymal stromal cells (MSCs) [27] MSCs are known tohave the ability to differentiate into multiple cell types includingosteoblasts Osteoblasts are the main cells responsible for synthe-sizing the mineralized compartment of bone tissue This processis regulated by among other molecules BMP-2 [3] PLGA parti-cles loaded with BMP-2 have been extensively used as has beendescribed and reviewed elsewhere [328ndash31]
Thus within this context it was the aim of the current study tooptimize the formulation and properties of a nanoparticle systemwith potential therapeutic applications Two different strategies toobtain PLGA-surfactant NPs were tested by using lysozyme as amodel for BMP-2 The size and morphology polydispersity indexzeta potential colloidal stability and encapsulation efficiency (EE)of the protein were analyzed
Once the physico-chemical characterization was completed thestudy was focused on the protein-release process using differ-ent techniques to study the results of in vitro experiments andfocusing it on the release pattern and the biological activity of thelysozyme released In this way a new formulation was establishedto develop a PLGA nanosystem with a singular dual size distribu-tion and the adequate balance between encapsulation and releaseof biologically active proteins Finally the effects of the proposedPLGA system were tested on primary MSCs in vitro as a proof ofconcept
2 Materials and methods
21 Formulation of the nanoparticles
Poly(lactide-co-glycolide) acid (PLGA 5050) ([C2 H2 O2]x [C3H4 O2]y) x = 50 y = 50 (Resomerreg 503H) 32ndash44 kDa was used asthe polymer The polymeric surfactant Pluronicreg F68 (Poloxamer188) (Sigma-Aldrich) was used as the emulsifier The structureis based on a poly(ethylene oxide)-block-poly(propylene oxide)-block-poly(ethylene oxide) and it is expressed as PEOa-PPOb-PEOawith a = 75 and b = 30 Lysozyme from chicken egg white (Sigma-L7651) was used as hydrophilic protein Water was purified ina Milli-Q Academic Millipore system Two different formulationmethods were developed termed O-F68 and W-F68
In the O-F68 method 25 mg of PLGA and 15 mg of F68 were dis-solved in 660 L of dichloromethane (DMC) and vortexed Then330 L of acetone were added and vortexed Next 100 L of abuffered solution at pH 128 with or without lysozyme (5 mgmL)were added dropwise while vortexing for 30 s Immediately thisprimary wateroil (WO) emulsion was poured into a glass con-taining 125 mL of ethanol under magnetic stirring and 125 mLof MilliQ water were added After 10 min of magnetic stirring the
organic solvents were rapidly extracted by evaporation under vac-uum until the sample reached a final volume of 10 mL
In the W-F68 method 100 mg of PLGA were dissolved in a tubecontaining 1 mL of ethyl acetate (EA) and vortexed 40 L of abuffered solution at pH 128 with or without lysozyme (20 mgmL)were added and immediately sonicated (Branson Ultrasonics 450Analog Sonifier) fixing the Duty cycle dial at 20 and the Outputcontrol dial at 4 for 1 min with the tube surrounded by ice This pri-mary WO emulsion was poured into a plastic tube containing 2 mLof a buffered solution (pH 128) of F68 at 1 mgmL and vortexingfor 30 s Then the tube surrounded by ice was sonicated again atthe maximum amplitude for the micro tip (Output control 7) for1 min This second WOW emulsion was poured into a glass con-taining 10 mL of the buffered F68 solution and kept under magneticstirring for 2 min The organic solvent was then rapidly extractedby evaporation under vacuum to a final volume of 8 mL
22 Cleaning and storage
After the organic solvent evaporation the sample was cen-trifuged for 10 min at 20 C at 14000 or 12000 rpm for O-F68 andW-F68 methods respectively The supernatant was filtered using100 nm filters for measuring the free non-encapsulated protein Thepellet was then resuspended in PB up to a final volume of 4 mL andkept under refrigeration at 4 C
221 Protein loading and encapsulation efficiencyThe initial protein loading was optimized for the nanoparticle
formulation preserving the final colloidal stability after the evapo-ration step and being different for each nanosystem Also 16 ww(LysPLGA) was used for O-F68 and 08 ww (LysPLGA) for W-F68one The amount of encapsulated lysozyme was calculated by mea-suring the difference between the initial amount added and the freenon-encapsulated protein which was tested by bicinchoninic acidassay (BCA Sigma-Aldrich) Then protein encapsulation efficiency(EE) and final drug loading (DL) was calculated as follows
EE = MI minus MF
MItimes 100 DL = MI minus MF
Mpolymertimes 100
where MI the initial total mass of Lys MF is the total mass of Lys inthe aqueous supernatant and Mpolymer is the mass of PLGA in theformulation
23 Characterization of the nanoparticles
231 Interfacial characterization of the first water-in-oilemulsion
The surface tension and dilatational rheology measurementsat the air-water interface were made in the OCTOPUS [32] aPendant Drop Surface Film Balance equipped with a subphasemulti-exchange device (patent submitted P201001588) describedin detail elsewhere [33] Here air plays the role of the organic phaseThe surface tension is calculated with DINATENreg software basedon axisymmetric drop shape analysis (ADSA) and the dilatationalmodulus (E) of the interfacial layer is determined from image anal-ysis with the program CONTACTOreg The in vitro model is describedin ldquoSupplementary materialrdquo
232 Particle morphologyNanoparticles were imaged by Scanning Electron Microscopy
(SEM) and Scanning Transmission Electron Microscopy (STEM)using a Zeiss SUPRA 40VP field emission scanning electron micro-scope from the Centre for Scientific Instrumentation of theUniversity of Granada (CIC UGR)
588 I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595
233 Nanoparticle size and electrokinetic mobilityThe hydrodynamic diameter and electrophoretic mobility of the
NPs were determined by using a Zetasizer NanoZeta ZS device(Malvern Instrument Ltd UK) working at 25 C with a He-Ne laserof 633 nm and a scattering angle of 173 Each data point wastaken as an average over three independent sample measurementsThe size of the NPs was characterized by Dynamic Light Scattering(DLS) The average hydrodynamic diameter (Z-average or cumu-lant mean) and the polydispersity index (PDI) were computedThese parameters are calculated through a cumulant analysis ofthe data which is applicable for narrow monomodal size distribu-tions [34] We also determined the intensity size distribution froman algorithm provided by the Zetasizer software (General Purpose)
The electrophoretic mobility was determined by the techniqueof Laser Doppler Electrophoresis An electrophoretic mobility dis-tribution as well as an average electrophoretic mobility (-average)was established for each sample
The hydrodynamic size distribution of the NPs with wide sizedistributions from DLS was also measured by using Nanoparti-cle Tracking Analysis (NTA) in a NanoSight LM10-HS(GB) FT14(NanoSight Amesbury United Kingdom) All samples were mea-sured more than three times for 60 s with manual shutter gainbrightness and threshold adjustments at 25 C The average sizedistribution (particle concentration vs diameter) was calculatedas an average of at least three independent size distributions
234 Nuclear magnetic resonance (NMR) of the nanoparticlesThe 1HNMR spectra of free F68 lysozyme-loaded particles from
O-F68 method with and without F68 and lysozyme-loaded parti-cles from W-F68 method were measured with a VNMRS 500 MHzspectrometer (Agilent) in the Centre for Scientific Instrumentation(CIC) of the University of Granada
24 Colloidal and temporal stability in biological media
The average hydrodynamic diameter and the polydispersityindex (PDI) by DLS of each system were measured to determinetheir colloidal stability in different media (Phosphate buffer [PB]Phosphate buffer saline [PBS] and cell culture medium Dulbeccorsquosmodified Eaglersquos medium [DMEM] from Sigma) and at differenttimes after (0 1 and 5 days)
In vitro release experiments were conducted following a simi-lar methodology as described above (Encapsulation efficiency) butusing 1 mL of each sample suspended in PBS at 37 C The proteinreleased from these samples was determined every 24 h by super-natant analysis and the pellet was suspended in the same volumeof buffer to maintain the release conditions All experiments weredeveloped in triplicate
241 Confocal microscopyLysozyme was labeled with fluorescein isothiocyanate (FITC)
using a method described by Kok et al [35] After FITC and lysozymecovalent conjugation concentrations were estimated spectropho-tometrically using the extinction coefficients described for FITC at494 nm and 280 nm The lysozyme concentration was calculatedmeasuring optical absorbance at 280 nm and subtracting the cor-responding FITC absorbance at this wavelength Images were madein a Nikon A1 laser scanning confocal microscope from CIC UGRAll experiments were performed in triplicate and replicated at leasttwice
25 Biological activity and interactions
251 Lysozyme biological activityThe biological activity of lysozyme was analyzed by an enzy-
matic activity kit (Sigma-Aldrich) using Micrococcus lysodeikticuscells as the substrate following the manufacturerrsquos instructions
252 Cellular uptakePrimary human mesenchymal stem cells (hMSCs) were taken
from healthy maxillary alveolar bone according to previouslydescribed protocols [36] After confirming their phenotype byflow cytometry and trilineage differentiation tests 12000 cellsper well were cultivated in sterile plates with glass bottom(Ibidi cat n 81158) overnight These cells were treated withmedium without fetal bovine serum (FBS) and Cell Tracker Red(15000) (C34552 ThermoFisher) for 30 min Then the mediumwas removed and supplemented with 10 FBS after which theparticles with lysozyme-FITC were added Then the hMSCs wereincubated 30 min again washed three times with PBS 1X and freshmedium supplemented with 2 FBS added Finally the hMSCs wereexamined by a confocal microscope (Nikon Eclipse Ti-E) Cell cul-tures were in all cases maintained at 37 C and 5 CO2 atmosphere
3 Results and discussion
31 Formulation of the nanoparticles
The methods developed in this work are intended to improvethe existing formulation techniques for hydrophilic protein loaded-PLGA NPs based on a double-emulsion process [1022] The noveltyof these methods is the use of the polymeric surfactant F68 either inthe organic phase (O-F68 method) or in the aqueous phase (W-F68)This surfactant reduces the size of the NPs enhances their stabilityand protects the encapsulated protein In addition the presence ofF68 on the surface of the particles reduces the recognition of thenanocarriers by the mononuclear phagocytic system (MPS) [37]
Additionally the choice of the organic solvent significantlyaffects the properties of the final colloidal system since the organicsolvent solubility regulates the inner and surface structure of theparticle In addition the interaction of the solvent with the encap-sulated biomolecule can alter its bioactivity as a consequence ofits denaturation as found for methylene chloride [26] In the O-F68method DMC is chosen as the organic solvent due to its lower watersolubility to facilitate the emulsification process and its low boil-ing point for easy evaporation However a freely water-miscibleorganic solvent (acetone) and the emulsifier F68 were added inthis organic phase to reduce its negative biological effects on theencapsulated protein [24] This emulsifier also reduces the protein-hydrophobic PLGA matrix interaction and thus the disruption ofthe protein structure [3] By contrast in the W-F68 method ethylacetate was used as the organic solvent which exerts less denatur-izing effects on the encapsulated protein [38] The higher watersolubility of this solvent favors rapid solvent removal The sol-vent removal rate is also accelerated by increasing the shear stressduring the second emulsification step It also enhances the encap-sulation efficiency and minimizes the contact time between theprotein and organic solvent [3] Poloxamer F68 is introduced in theexternal aqueous phase
Both formulations (O-F68 and W-F68) (Table 1) gave rise to col-loidally stable samples and the encapsulation of lysozyme insidethe nanoparticles in agreement with the double WOW emulsionmethod [23] Lysozyme was chosen as a model protein due to itsbiostability well-known characteristics and ease in quantifying itsbiological activity [3940] In addition its molecular size (143 kD)and its basic isoelectric point (around pH = 11) make it an appro-
I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595 589
Table 1Formulation conditions and protein encapsulation results PLGA F68 and LYSI are the initial amount of polymer surfactant and lysozyme respectively Initial is the initialpolymer-protein rate in ww EE is the encapsulation efficiency LYSF is the final encapsulated amount of lysozyme DL is the final drug loading rate in ww
PLGA (mg) F68 (mg) LYSI (mg) Initial EE LYSF (mg) DL
O-F68-Lys 25 15 04 16 625 025 1W-F68-Lys 100 2 08 08 731 058 058
priate model for other proteins such as bone-growth factors [15]Three main objectives drove the optimization of the appropriaterelation among the polymer poloxamer and protein (1) to havecolloidally stable nanosystems of submicron sizes (2) to encap-sulate a sufficient amount of protein and (3) to prevent proteindestabilization by maintaining their biological activity
Therefore regardless of the formulation method it wasintended to limit the initial protein loading to provide colloidallystable nanosystems In our case as shown in Table 1 Initial val-ues were the best choice to maintain colloidal stability withoutsignificantly changing the size distribution (see below) In con-sequence DL presents relative low values for both formulationsalthough the encapsulated amount of lysozyme LYSF is greaterthan those required for therapeutic proteins with lower clinicallyeffective amounts [41] The value of EE found for O-F68-Lys NPsis in consonance with the formulation characteristics and simi-lar to other reports with different proteins [12104214] includingbovine serum albumin (BSA) or insulin [1242] and several growthfactors [14]
The presence of surfactant stabilizes the emulsion droplets andreduces their size However it also alters the protein-polymerinteraction which translates into a reduction of the encapsulationefficiency This was evidenced by Blanco et al when encapsulat-ing BSA and lysozyme in different PLGA-poloxamer microparticles[10] Moreover the type of protein and its initial theoretical loadingare factors directly related with the EE and can affect the colloidalstability of the primary emulsion as shown by Santander et al [12]The different polymersurfactant ratio between the two formula-tions is not comparable since the surfactant is added in a differentway In both cases we used previous formulations as the startingpoint [1022] and tested several polymersurfactant ratios (datanot shown) in order to obtain the best colloidal stability EE andDL In Table 1 we show the data for the optimized PLGAF68 ratiosin both systems
In the W-F68 method despite the higher EE value with respectto O-F68 system an almost complete encapsulation was expecteddue to the low initial proteinPLGA mass ratio [12] and to theabsence of surfactant in the first emulsion step The characteris-tics of the modified formulation process may have the key In thisformulation the relatively high solubility of the ethyl acetate inwater promotes rapid diffusion of the organic solvent into the sec-ond aqueous phase An initial small volume of water containingpoloxamer is initially added to prevent a rapid uncontrolled precip-itation of the polymer and to control the speed of the process Thisis subsequently supplemented with the addition of a larger aque-ous volume as previously described [26] When this solidificationis slow it favors the escape of the protein and the EE decreasesHowever if the solidification is very fast the contact of proteinwith the organic solvent is minimized and the EE increases On thenegative side it can produce polymer agglomeration which inter-feres with the correct formation of the NPs The introduction of anintermediate step with a reduced volume of aqueous phase withpoloxamer can modulate the rate of the process by controlling thediffusion of ethyl acetate into the water and by allowing the diffu-sion into the organic phase of the poloxamer A controlled velocityof the polymer pre-solidification process in the presence of surfac-tant can produce channels or pores in the polymeric shell that onone hand could facilitate the protein release and on the other hand
could drive down the EE value [43] As a result of these phenom-ena the final DLs (ww of lysozymepolymer) shown in Table 1 forboth NP systems are suitable for their application as nanotransportsystems
32 Characterization of the nanoparticles
321 Interfacial characterization of the first water-in- oilemulsion
To gain better insight into the effect of the formulation methodon the interfacial properties of the first water (lysozyme solution)-in-oil emulsion we designed surface experiments with lysozymeand Pluronicreg F68 The main difference in the two formulationmethods is how the Pluronicreg F68 is added in aqueous phase(WndashF68) or in organic phase (O-F68) This difference could affectthe composition of the surface of the NPs and as a result theircolloidal properties
The surface tension and elasticity at the air-water interfacewere the properties analyzed (Table 2) At this interface proteinschange their conformation and expose their hydrophobic part toair depending on their thermodynamic stability flexibility amphi-pathicity molecular size and charge In our case lysozyme is aglobular protein that is adsorbed at the air-water interface andforms a rigid monolayer due to its internal structure and the pres-ence and number of disulfide bridges [44] Our measurements weremade at pH 12 thus lysozyme is negatively charged Table 2 showsthe interfacial tension of the lysozyme monolayer at the air-waterinterface after 50 min of adsorption (457 plusmn 04 (mNm)) and itselasticity (83 plusmn 4 (mNm)) The reduction of the interfacial tensionwhen compared with that of the air-water interface (72 mNm)indicates the surfactant characteristics of the lysozyme The highvalue of elasticity was due to the charge and high molecular inter-actions in the lysozyme monolayer When the monolayer is formedwith Pluronicreg F68 the surface tension is slightly lower than withlysozyme when the Pluronicreg is added in AP but similar (takinginto account the error) when added in OP
Pluronicreg F68 is an amphiphilic molecule that is adsorbed at theair-water interface when it is dissolved in aqueous phase and alsowhen it is deposited onto the surface of the drop Small differencesare found when comparing the surface tension of the Pluronicreg
monolayer from the two methods The different values of interfa-cial tension attained in both cases would be due to the differentmethods to add the Pluronicreg F68 at the formed lysozyme mono-layer Pluronicreg F68 presents lower elasticity than the lysozyme asexpected since Pluronicreg F68 is known to form a flexible monolayerat the air-water interface [45]
Two assays were designed to mimic the formulation methodsof the particles In the first assay (W-F68 method) a monolayer oflysozyme was formed then the bulk of the drop was exchangedwith the aqueous solution of Pluronicreg F68 and after adsorptionthe interfacial tension and elasticity of the interface were mea-sured (379 plusmn 06 mNm and 142 plusmn 05 mNm respectively) Thislow value of elasticity was very similar to that of the monolayerof Pluronicreg F68 indicating that Pluronicreg F68 is located at theinterface and removes the previously adsorbed lysozyme In thesecond assay (O-F68 method) after the monolayer of lysozyme wasformed the Pluronicreg F68 dissolved in chloroform is deposited ontothe surface of the drop The chloroform is rapidly evaporated and
590 I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595
Table 2Interfacial tension and dilatational elasticity (at 1 Hz) of the air-water interface (a) after adsorbing lysozyme or Pluronicreg F68 in the aqueous phase (AP) or Pluronicreg F68 inorganic phase (OP) in the first step (b) when Pluronicreg F68 is added in AP or OP after adsorption of lysozyme monolayer (mean plusmn sd n = 3)
First step Interfacial Tension(mNm)
Elasticitya
(mNm)Second step Interfacial Tension
(mNm)Elasticityb
(mNm)
Lysozyme 457 plusmn 04 83 plusmn 4 Pluronicreg F68 (AP) 379 plusmn 06 142 plusmn 05Lysozyme 457 plusmn 04 83 plusmn 4 Pluronicreg F68 (OP) 38 plusmn 2 43 plusmn 4Pluronicreg F68 (AP) 421 plusmn 03 15 plusmn 3Pluronicreg F68 (OP) 475 plusmn 21 94 plusmn 05
the interfacial tension and elasticity of the interface are measured(38 plusmn 2 mNm and 43 plusmn 4 mNm respectively) The elasticity washalf of that of the pure lysozyme monolayer perhaps because ofthe coexistence of lysozyme and Pluronicreg F68 molecules at theinterface The surface tension of the final interface does not dependon the method of adding the Pluronicreg but it is lower than that ofthe pure lysozyme or the pure Pluronicreg
Within this context it has been widely reported that the adsorp-tion of PEO and poloxamers at the interface reduces the proteinbinding [4647] In the O-F68 method the lysozyme is exposedto the DCM after the formation of the first water-in-oil emulsioneven if Pluronicreg is added as they both coexist at the interface Inthe W-F68 method protein will be in contact with ethyl acetate inthis step as Pluronicreg is absent However this solvent has weakerbiological effects on lysozyme Pluronicreg could reach the interfacewhen added to the aqueous phase in the following step and dis-place the protein from the interface which could diffuse outwardsto the aqueous phase
322 Particle morphologyThe delivery biodistribution and action mechanism of a trans-
ported drug or biomolecule depend heavily on the size of theparticle concentration and timing [48] In general the micromet-ric scale is designed for a local supply that allows the formation ofreservoirs of the transported molecule and minimizes the actionof the phagocytic system [49] However nanometric systems aremore versatile because they permit a systemic distribution aremore stable and reactive and allow extra- as well as intracellu-lar action This latter mechanism is essential when the moleculeor drug should act in the cytoplasm [50] or any other intracellularstructure such as the mitochondria Golgi apparatus endoplasmicreticulum or nucleus [485152] Other parameters to alter the intra-cellular fate of the particles have also been investigated mainly byaltering their surface decoration [53] for example with nuclearlocalization signals (NLS) that use the nucleus as the target of theparticle [51] However these strategies are still in their very earlydevelopmental phase [4852]
A particle size in the submicron scale (between 2 and 500 nm)was sought as it is necessary for cell internalization and a rapiddistribution after parenteral administration in order to reach dif-ferent tissues through different biological barriers Particles under200 nm minimize their intake by macrophages The type of organicsolvent the polymer concentration the addition of surfactant andthe emulsification energy control the size of the system
The O-F68 method gives rise to a monomodal particle-size dis-tribution with diameters around 100 nm The addition of Pluronicreg
F68 in the organic phase bolsters colloidal stability of the first emul-sion and reduces the particle size in comparison with PLGA NPsin which the stability is purely electrostatic due to the carboxylicgroups of the PLGA In the W-F68 method shear stress and volumeof the aqueous phase are taken into account to produce a systemwith particles of between 100 and 500 nm
O-F68-Lys NPs have a spherical shape with a monomodal sizedistribution (diameters around 100 nm) and core-shell structure(Fig 1a) Empty particles produced with the O-F68 method are
shown in Figs S1 (without F68) and S2 (with F68) They are alsospherical and with a core-shell structure but slightly larger
W-F68-Lys NPs also present a spherical shape but a multi-modal size distribution with diameters between 140 and 450 nmthe largest population being around 260 nm (Fig 1b) A core-shellstructure is also observed in these particles Empty particles fromthe W-F68 method are presented in Fig S3 corresponding to a morepolydisperse system
323 Nanoparticle size electrokinetic mobility and colloidalstability
The hydrodynamic diameter distribution of the particles wasdetermined firstly by DLS Table 3 contains the main colloidal prop-erties of particles produced with the O-F68 and W-F68 methodsempty or loaded with lysozyme The results of empty particles fromthe O-F68 method but synthesized without F68 are also included
The size parameters were calculated through a cumulative anal-ysis of the data which is applicable for narrow monomodal sizedistributions [34] SEM and STEM micrographs indicate that suchan approximation could be assumed for particles from the O-F68method but not from the W-F68 one Thus the intensity size distri-butions of the different systems are shown in Fig 2a The presenceof Pluronicreg F68 in the O-F68 method significantly reduces the sizeand polydispersity of the NPs This agrees with the reduction of thesurface tension when the F68 is at the interface (Table 2) whichpromotes the emulsification process If the NPs are also loaded withlysozyme the size is even smaller but the polydispersity increasesslightly compared with the empty particles The surfactant prop-erties of the lysozyme have been shown with the surface-tensionresults (Table 2)
Fig 2a indicates the presence of particles higher than 500 nmwith the W-F68 which does not correlate with the SEM micro-graphs Thus a different technique (NTA) was used to gaininformation on the size distribution of such systems (Fig 3b) WithNTA the size distribution was consistent with the SEM imagesBroad size distributions corresponding to multimodal systemswere found with this method but the addition of lysozyme ledto a clear size reduction This is because lysozyme also acts as anemulsifier in the first emulsion
The electrokinetic charge of the NPs was analyzed by measuringthe electrophoretic mobility For comparison all the samples weremeasured at pH 7 (phosphate buffer) In Fig 3 the electrophoreticmobility distributions are presented while the corresponding -averages are shown in Table 3
PLGA NPs are usually negatively charged due to the carboxylicgroups of the polymer The use of Pluronicreg F68 in the O-F68method clearly reduces the electrophoretic mobility of the NPswhich indicates that some Pluronicreg is located at the NP sur-face This reduction was expected after the incorporation of thisnon-ionic surfactant onto the interface since the presence ofpolyethylene oxide chains would cause an outward shift of theshear plane where the -potential is defined and this would sub-sequently diminish electrophoretic mobility Previous results forPLGA particles have shown a significant reduction directly relatedto the poloxamer coating [54] If we compare the two systems the
I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595 591
Fig 1 SEM and STEM micrographs of lysozyme-loaded particles using O-F68 (a) or W-F68 method (b)
Table 3Colloidal properties of PLGA NPs from different formulation methods They were measured in phosphate buffer (pH 7) The average hydrodynamic diameter (Z-average orcumulative mean) and the polydispersity index (PDI) are determined from DLS (Mean plusmn sd n = 3)
Z-average (nm) PDI -average (mcmVs)
O-F68 method Empty without F68 266 plusmn 7 0293 minus506 plusmn 015Empty 1627 plusmn 21 0081 minus429 plusmn 018Lysozyme-loaded 1210 plusmn 12 0244 minus334 plusmn 007
W-F68 method Empty 273 plusmn 3 0193 minus531 plusmn 011Lysozyme-loaded 293 plusmn 4 0169 minus4212 plusmn 0013
Fig 2 Hydrodynamic diameter distribution (a) by DLS at pH 7 (phosphate buffer) of empty and lysozyme-loaded PLGA particles from the O-F68 and W-F68 methods and(b) by NTA at pH 7 (phosphate buffer) of empty and lysozyme-loaded PLGA particles from the W-F68 method
less negative surface for OF68 NPs would be related to less densityof surface PLGA polymer bringing the negative electrical charge tothe interface This result would be in line with the greater amountof PLGA in the formulation of WF68 nanosystem
When the lysozyme is also used in the synthesis the surfaceis even less negative which could be explained by the presence
of some protein (whose net charge is positive) near or at theinterface This latter effect is also found with the W-F68 methodThe attractive electrostatic interaction between negative terminalacid residues of PLGA and lysozyme molecules plays a key rolein the process of protein encapsulation [41] or adsorption [40]in PLGA NPs which affects the final protein loading In relation
592 I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595
Fig 3 Electrophoretic mobility distribution at pH 7 (phosphate buffer) of empty and lysozyme-loaded PLGA particles from the (a) O-F68 and (b) W-F68 methods
to this situation an important characteristic of the W-F68 encap-sulation formulation is that the water phase is at pH 12 whichallows a negative net charge of lysozyme and thus avoids theelectrostatic protein-polymer attraction This situation can reducethe encapsulation efficiency but at the same time favors the laterprotein-diffusion process and consequently the short-term release
Recent studies have proposed the use of nanoparticles embed-ded in predesigned 3D-printed scaffolds [5556] moving us toanalyze the stability of the two formulations in several mediausually employed during the preparation of other structures Sizedistributions similar to the original were found for the two formula-tions in different media (PB PBS and DMEM) and at different timesafter synthesis (0 1 and 5 days) The electric charge of PLGA acidend groups and the poloxamer molecules located on the NP sur-face confers a combined electrostatic and steric colloidal-stabilitymechanism as has previously been described [4654] Additionallythe NPs in all cases keep their size under storage at 4 C at least for1 month (data not shown) Thus the media described could poten-tially be used as storage media or to prepare other solutions orscaffolds before actually placing them in the living environment(in vitro or in vivo)
324 NMR of the nanoparticlesIn Fig 3 both empty and protein-loaded NPs present less neg-
ative electrophoretic mobility than do empty NPs without F68which could be explained by the presence of Pluronicreg F68 atthe surface of the NP By comparing the 1HNMR spectra of freePluronicreg F68 and lysozyme-loaded NPs from O-F68 and W-F68methods we can check the presence of F68 at the surface of theNPs (Fig S4) by the peaks shown between 325 and 375 ppm and at1 ppm These peaks are also visible in the spectra of NPs formulatedwith F68 (O-F68 and W-F68 Figs S5 and S6 respectively)
33 Biological activity and interactions
A controlled release from a PLGA-based delivery system is adifficult task as it depends on multiple factors the type of PLGAsolvent mechanical stress use of surfactants etc [57] The diffusionof the protein and the polymer erosion are the main mechanismsinvolved in the protein release in PLGA-based delivery systems Fur-thermore it is typical to find a rapid burst release at the initial stagefollowed by a slow release phase over the short and medium termIn this phase protein molecules diffuse through the polymer matrixuntil reaching a final phase in which the polymer degradation byhydrolysis allows a faster release [20]
On the other hand the short-term release is of special inter-est for transporting bone morphogenetic growth factors (BMPs) Acontrolled initial burst followed by a sustained release significantlyimproves in vivo regeneration of bone [3] and cartilage [58] even in
Fig 4 Cumulative release (filled symbols) and residual bioactivity (open symbols)of O-F68-Lys (square) and W-F68-Lys (triangle) incubated for different times at 37 Cin saline phosphate buffer (pH 74) (mean plusmn sd n = 3)
dual-controlled release systems [59] For these reasons we focusedour analysis on short-term release taking into account the reducedpolymer degradation by hydrolysis found for similar systems forthese early steps [60]
Fig 4 shows the accumulative release of lysozyme from O-F68-Lys NPs over the short term (seven days) These results areconsistent with a two-stepped process an initial burst and a slow-release phase The first step could correspond to the release ofthe protein molecules located near surface whose presence wasdeduced from the electrophoretic mobility results (Fig 3) The sec-ond part of the release process was limited and slow due to theprotein diffusion through the matrix of the polymeric shell Thespecific electrostatic interaction between the positive lysozymemolecules and the PLGA negative terminal acid groups can reducethe protein diffusion [10] When the poloxamer (F68) is added theinteraction between the surfactant and the protein helps the diffu-sion process leading to a more complete and sustained release [12]It also helps to keep the biological activity of the protein [4161] Thepoloxamer reduces the non-specific protein-polymer interactions(ie hydrophobic interactions) but not the specific ones (electro-statics) thus the diffusion through water-filled pores or throughthe polymer is still limited In the current study the protein fractionreleased and the release pattern are similar to those found in theliterature for lysozyme encapsulated in nano- and microparticlesof blends of PLGA and other polymers or surfactants [261511]
The protein release curve from W-F68-Lys NPs (Fig 4) revealsthat the initial delivery rate is identical to that of the O-F68 systemwhich could mean a similar proportion of encapsulated proteinclose to or at the surface for both NP systems This would agreewith the analogous decrease in the electrophoretic mobility of the
I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595 593
Fig 5 z-projection of 5 images of hMSCs visualized 30 min after incubation with W-F68-LysFITC NPs or O-F68-LysFITC NPs hMSCs were previously labeled with cell-trackerred Scale bar 20 m
lysozyme-loaded NPs previously reported (Fig 3) In the secondpart of the process the specific interaction between the proteinand the polymer is again present However the diffusion processin the W-F68 system appears to be enhanced allowing a contin-uous and sustained release after the initial burst and reaching aslightly higher value for the maximum release time studied Thisresult could be related to the inner structure of the polymer layerthat allows better hydration and therefore better diffusion of theprotein towards the outside It has been previously reported thatthe use of less polar organic solvents such as DCM for PLGA par-ticles formulations increases the density of the polymer matrix incomparison with more polar organic solvents such as EA The PLGAmatrices prove more resistant in the first case but reducing at thesame time their connectivity and diffusivity [62] Meng et al [26]found that faster removal of EA results in a slower kinetic release ofthe protein due to a decrease in the porosity of the NPs Regardingthe role of the Pluronicreg Rafati et al [63] found a higher concentra-tion of protein encapsulated in the surface pores in microparticlessynthesized in the presence of surfactant in the second aqueousphase of the emulsion Since an intermediate step was introducedin our W-F68 formulation in the second aqueous phase of the emul-sion the removal of the EA by diffusion was strongly controlled sothat it was expected that the porosity of these NPs would increaseThis porosity improves protein diffusion which allows a more sta-ble release pattern according to the experimental result found forthis system Despite the unfavorable effect of the specific electro-static protein-polymer interaction on the release the amount ofreleased protein in our NPs is substantial signifying that there areother unspecific interactions that can be modulated by the pres-ence of surfactant allowing a sustained release The amount ofreleased lysozyme is similar to that found with lysozyme physi-cally adsorbed onto the surface of PLGA nanoparticles despite theelectrostatic attraction [40] Besides other unspecific interactionsthe electrolyte concentration in the release medium could modu-late this electrostatic attraction between the protein and polymerdiminishing it and facilitating the release process [46]
Another remarkable parameter is the biological activity of thein vitro release of lysozyme shown in Fig 4 While in the O-F68system the bioactivity is partially reduced by up to 40 the pro-
tein supplied by the W-F68 system maintains the activity above90 with respect to that of commercially supplied lysozyme andresuspended in the same release buffer As discussed above boththe organic solvent and the hydrophobic interaction between theprotein and the polymer often cause denaturation of encapsulatedproteins [4164] Perez et al [11] describe a partial loss of activ-ity when using DCM and an aqueous PVA solution in the secondemulsification step without any additional excipient The use ofpoloxamers in the formulation reduces such interactions enhancesthe stability of the protein and maintains an aqueous layer thatretains the water molecules necessary for the biological functionof the protein at the same time aiding its diffusion This situationtogether with the use of a weak organic solvent such as EA helpspreserve the biological activity of the lysozyme as found for theW-F68-Lys system
Fig S7 presents different confocal microscopy images relatedto the release process of lysozyme-loaded W-F68 NPs A decreasein fluorescence intensity was appreciable over the course of thein vitro experiment In addition the aggregation of the system isvisible as the incubation process progresses The analysis of theseimages is consistent with the previously reported results for thisNP system
331 Cellular uptakeCellular uptake of PLGA NPs is a known process affected
mainly by surface properties and functionalization [9] and parti-cle aggregation [65] Internalization and subsequent intracellularprocessing of the particles have been described as an activeprocess thus it is energy dependent and can therefore beaffected by other factors that alter the energy uptake by cellssuch as temperature [48] Particles can be internalized by sev-eral endocytosis methods dependent primarily on the size ofthe particle caveolin-dependent particles (diameter asymp 60 nm)clathrin-independent (diameter asymp 90 nm) and clathrin-dependent(diameter asymp 120 nm) [5152] Once internalized about 65 areexported back to the extracellular space before releasing anyof their content while the rest slowly release the encapsulatedmolecule into the intracellular space [66] The intracellular releaseprocess is affected by the formulation of the particles [48] We have
594 I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595
demonstrated that the proposed systems follow a pattern similar toothers previously published As early as 30 min after incubation W-F68-LysFITC NPs were taken up by the cells (Fig 5) Some W-F68particles were still in the medium so that the dual activity couldhappen In contrast O-F68-LysFITC NPs were affected by aggrega-tion and therefore did not properly reach the intracellular space(Fig 5 for z-axis images view Fig S8) This contradicts the previousanalyses of the colloidal stability in PB PBS and DMEM This findingcan be explained by the fact that although the culture media wasDMEM this latter medium was supplemented with fetal bovineserum and cells release many factors to the extracellular mediumthat can affect these types of particles None of the systems wereshown to be toxic for the cells (Fig S9) No studies available havereported any effects of lysozyme on hMSCs
4 Conclusions
A novel dual-delivery PLGA-nanosystem has been developedin which the formulation and components favor an adequateshort-term delivery pattern while preserving the bioactivity ofencapsulated molecules The analysis of the polymer-surfactant-protein interaction shows that the organic solvent use ofsurfactant volume relation of both phases and the net charge of theprotein play important roles in the final characteristics and releasebehavior of the nanoparticles The W-F68 formulation balances allof them in order to provide a nanosystem ready to transport anddeliver hydrophilic biomolecules such as proteins In vitro releaseexperiments display an adequate short-term delivery pattern thatat the same time preserves the bioactivity of the encapsulatedbiomolecule Additionally the singular nanoparticle size distribu-tion found for this W-F68 nanosystem allows the possibility of adual outer- and intra-cellular protein delivery as has been shownby in vitro cellular experiments This novel formulation will be usedin future studies to encapsulate and deliver growth factors in vitroand in vivo in order to exploit the therapeutic potential of thisnanosystem
Acknowledgements
The authors wish to express their appreciation for the tech-nical support to Dr Azahara Rata-Aguilar and for the financialsupport granted by the Consejeriacutea de Economiacutea Innovacioacuten Cienciay Empleo de la Junta de Andaluciacutea (Spain) through research groupsFQM-115 and CTS-1028 and by the following research projectMAT2013-43922-R ndash European FEDER support included minus (MICINNSpain)
Appendix A Supplementary data
Supplementary data associated with this article can be found inthe online version at httpdxdoiorg101016jcolsurfb201708027
References
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[3] Bone regeneration from PLGA micro-Nanoparticles Biomed Res Int (2015)httpwwwhindawicomjournalsbmriaa415289
[4] K-B Lee A Solanki J Kim J Jung Nanomedicine dynamic integration ofnanotechnology with biomedical science in Handb Clin Nanomedicine PanStanford 2016 2017 pp 21ndash60 httpdxdoiorg101201b19915-4
[5] GEJ Poinern A Laboratory Course in Nanoscience and Nanotechnology CRCPress Taylor amp Francis Group 2015
[6] MM Yallapu BK Gupta M Jaggi SC Chauhan Fabrication of curcuminencapsulated PLGA nanoparticles for improved therapeutic effects inmetastatic cancer cells J Colloid Interface Sci 351 (2010) 19ndash29 httpdxdoiorg101016jjcis201005022
[7] BP Nair CP Sharma Poly(lactide-co-glycolide)-laponite-F68 nanocompositevesicles through a single-step double-emulsion method for the controlledrelease of doxorubicin Langmuir 28 (2012) 4559ndash4564 httpdxdoiorg101021la300005c
[8] R Shankarayan S Kumar P Mishra Differential permeation ofpiroxicam-loaded PLGA micronanoparticles and their in vitro enhancementJ Nanopart Res 15 (2013) 1496 httpdxdoiorg101007s11051-013-1496-6
[9] JA Loureiro B Gomes G Fricker MAN Coelho S Rocha MC PereiraCellular uptake of PLGA nanoparticles targeted with anti-amyloid andanti-transferrin receptor antibodies for Alzheimerrsquos disease treatmentColloids Surf B Biointerfaces 145 (2016) 8ndash13 httpdxdoiorg101016jcolsurfb201604041
[10] D Blanco MJ Alonso Protein encapsulation and release frompoly(lactide-co-glycolide) microspheres effect of the protein and polymerproperties and of the co- encapsulation of surfactants Eur J PharmBiopharm 45 (1998) 285ndash294 httpdxdoiorg101016S0939-6411(98)00011-3
[11] C Peacuterez P De Jesuacutes K Griebenow Preservation of lysozyme structure andfunction upon encapsulation and release from poly(lactic-co-glycolic) acidmicrospheres prepared by the water-in-oil-in-water method Int J Pharm248 (2002) 193ndash206 httpdxdoiorg101016S0378-5173(02)00435-0
[12] MJ Santander-Ortega N Csaba L Gonzaacutelez D Bastos-Gonzaacutelez JLOrtega-Vinuesa MJ Alonso Protein-loaded PLGAndashPEO blend nanoparticlesencapsulation release and degradation characteristics Colloid Polym Sci288 (2010) 141ndash150 httpdxdoiorg101007s00396-009-2131-z
[13] N Pirooznia S Hasannia A Lotfi M Ghanei Encapsulation of alpha-1antitrypsin in PLGA nanoparticles in Vitro characterization as an effectiveaerosol formulation in pulmonary diseases J Nanobiotechnol 10 (2012) 20httpdxdoiorg1011861477-3155-10-20
[14] I DrsquoAngelo M Garcia-Fuentes Y Parajoacute A Welle T Vaacutentus A Horvaacuteth GBoumlkoumlnyi G Keacuteri MJ Alonso Nanoparticles based on PLGA poloxamer blendsfor the delivery of proangiogenic growth factors Mol Pharm 7 (2010)1724ndash1733 httpdxdoiorg101021mp1001262
[15] LJ White GTS Kirby HC Cox R Qodratnama O Qutachi FRAJ Rose KMShakesheff Accelerating protein release from microparticles for regenerativemedicine applications Mater Sci Eng C 23 (2013) 2578ndash2583 httpdxdoiorg101016jmsec201302020
[16] P Pantazis K Dimas JH Wyche S Anant CW Houchen J Panyam RPRamanujam Preparation of siRNA-Encapsulated PLGA nanoparticles forsustained release of siRNA and evaluation of encapsulation efficiencyNanopart Biol Med (2012) 311ndash319 httpdxdoiorg101007978-1-61779-953-2 25 Humana Press Totowa NJ
[17] JS Park HN Yang DG Woo SY Jeon KH Park Multilineage differentiationof human-derived dermal fibroblasts transfected with genes coated on PLGAnanoparticles plus growth factors Biomaterials 34 (2013) 582ndash597 httpdxdoiorg101016jbiomaterials201210001
[18] F Wan M Yang Design of PLGA-based depot delivery systems forbiopharmaceuticals prepared by spray drying Int J Pharm 498 (2016)82ndash95 httpdxdoiorg101016jijpharm201512025
[19] A Giteau MC Venier-Julienne A Aubert-Poueumlssel JP Benoit How to achievesustained and complete protein release from PLGA-based microparticles IntJ Pharm 350 (2008) 14ndash26 httpdxdoiorg101016jijpharm200711012
[20] S Fredenberg M Wahlgren M Reslow A Axelsson The mechanisms of drugrelease in poly(lactic-co-glycolic acid)-based drug delivery systemsmdashareview Int J Pharm 415 (2011) 34ndash52 httpdxdoiorg101016jijpharm201105049
[21] F Mohamed CF van der Walle Engineering biodegradable polyesterparticles with specific drug targeting and drug release properties J PharmSci 97 (2008) 71ndash87 httpdxdoiorg101002jps21082
[22] N Csaba L Gonzaacutelez A Saacutenchez MJ Alonso Design and characterisation ofnew nanoparticulate polymer blends for drug delivery J Biomater Sci PolymEd 15 (2004) 1137ndash1151 httpdxdoiorg1011631568562041753098
[23] HK Makadia SJ Siegel Poly lactic-co-glycolic acid (PLGA) as biodegradablecontrolled drug delivery carrier Polymers (Basel) 3 (2011) 1377ndash1397 httpdxdoiorg103390polym3031377
[24] F Danhier E Ansorena JM Silva R Coco A Le Breton V Preacuteat PLGA-basednanoparticles an overview of biomedical applications J Controlled Release161 (2012) 505ndash522 httpdxdoiorg101016jjconrel201201043
[25] G Ratzinger U Laumlnger L Neutsch F Pittner M Wirth F Gabor Surfacemodification of PLGA particles the interplay between stabilizer ligand sizeand hydrophobic interactions Langmuir 26 (2010) 1855ndash1859 httpdxdoiorg101021la902602z
[26] FT Meng GH Ma W Qiu ZG Su WOW double emulsion technique usingethyl acetate as organic solvent effects of its diffusion rate on thecharacteristics of microparticles J Controlled Release 91 (2003) 407ndash416httpdxdoiorg101016S0168-3659(03)00273-6
[27] M Padial-Molina F OrsquoValle A Lanis F Mesa DM Dohan Ehrenfest H-LWang P Galindo-Moreno Clinical application of mesenchymal stem cells andnovel supportive therapies for oral bone regeneration Biomed Res Int 2015(2015) httpdxdoiorg1011552015341327
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[28] P Yilgor N Hasirci V Hasirci Sequential BMP-2BMP-7 delivery frompolyester nanocapsules J Biomed Mater Res ndash Part A 93 (2010) 528ndash536httpdxdoiorg101002jbma32520
[29] B Li T Yoshii AE Hafeman JS Nyman JC Wenke SA Guelcher The effectsof rhBMP-2 released from biodegradable polyurethanemicrospherecomposite scaffolds on new bone formation in rat femora Biomaterials 30(2009) 6768ndash6779 httpdxdoiorg101016jbiomaterials200908038
[30] Y Wang Y Wei X Zhang M Xu F Liu Q Ma Q Cai X Deng PLGAPDLLAcore-shell submicron spheres sequential release system preparationcharacterization and promotion of bone regeneration in vitro and in vivoChem Eng J 273 (2015) 490ndash501 httpdxdoiorg101016jcej201503068
[31] YB Shim HH Jung JW Jang HS Yang H Bae JC Park B Choi SH LeeFabrication of hollow porous PLGA microspheres using sucrose for controlleddual delivery of dexamethasone and BMP2 J Ind Eng Chem 37 (2016)101ndash106 httpdxdoiorg101016jjiec201603014
[32] J Maldonado-Valderrama JAH Terriza A Torcello-Goacutemez MACabrerizo-Viacutelchez In vitro digestion of interfacial protein structures SoftMatter (2013) 1043ndash1053 httpdxdoiorg101039c2sm26843d
[33] M a Cabrerizo-Vilchez H a Wege J a Holgado-Terriza a W NeumannAxisymmetric drop shape analysis as penetration Langmuir balance Rev SciInstrum 70 (1999) 2438ndash2444 httpdxdoiorg10106311149773
[34] PA Hassan S Rana G Verma Making sense of brownian motion colloidcharacterization by dynamic light scattering Langmuir 31 (2015) 3ndash12httpdxdoiorg101021la501789z
[35] RJ Kok M Haas F Moolenaar D de Zeeuw DK Meijer Drug delivery to thekidneys and the bladder with the low molecular weight protein lysozymeRen Fail 20 (1998) 211ndash217
[36] S Mason SA Tarle W Osibin Y Kinfu D Kaigler Standardization and safetyof alveolar bone-derived stem cell isolation J Dent Res 93 (2014) 55ndash61httpdxdoiorg1011770022034513510530
[37] C Farace P Saacutenchez-Moreno M Orecchioni R Manetti F Sgarrella Y AsaraJM Peula-Garciacutea JA Marchal R Madeddu LG Delogu Immune cell impactof three differently coated lipid nanocapsules pluronic chitosan andpolyethylene glycol Sci Rep 6 (2016) 18423 httpdxdoiorg101038srep18423
[38] C Sturesson J Carlfors Incorporation of protein in PLG-microspheres withretention of bioactivity J Controlled Release 67 (2000) 171ndash178 httpdxdoiorg101016S0168-3659(00)00205-4
[39] L Ying S Jiali J Guoqiang Z Jia D Fuxin In vitro evaluation oflysozyme-loaded microspheres in thermosen- sitive methylcellulose-basedhydrogel Chin J Chem Eng 15 (2007) 566ndash572
[40] C Cai U Bakowsky E Rytting AK Schaper T Kissel Charged nanoparticlesas protein delivery systems a feasibility study using lysozyme as modelprotein Eur J Pharm Biopharm 69 (2008) 31ndash42 httpdxdoiorg101016jejpb200710005
[41] A Paillard-Giteau VT Tran O Thomas X Garric J Coudane S Marchal IChourpa JP Benoicirct CN Montero-Menei MC Venier-Julienne Effect ofvarious additives and polymers on lysozyme release from PLGA microspheresprepared by an sow emulsion technique Eur J Pharm Biopharm 75 (2010)128ndash136 httpdxdoiorg101016jejpb201003005
[42] MJ Santander-Ortega D Bastos-Gonzaacutelez JL Ortega-Vinuesa MJ AlonsoInsulin-loaded PLGA nanoparticles for oral administration an in vitrophysico-chemical characterization J Biomed Nanotechnol 5 (2009) 45ndash53httpdxdoiorg101166jbn2009022
[43] ID Rosca F Watari M Uo Microparticle formation and its mechanism insingle and double emulsion solvent evaporation J Controlled Release 99(2004) 271ndash280 httpdxdoiorg101016jjconrel200407007
[44] S Pezennec F Gauthier C Alonso F Graner T Croguennec G Bruleacute ARenault The protein net electric charge determines the surface rheologicalproperties of ovalbumin adsorbed at the air-water interface FoodHydrocolloids 14 (2000) 463ndash472 httpdxdoiorg101016S0268-005X(00)00026-6
[45] A Torcello-Goacutemez MJ Santander-Ortega JM Peula-Garciacutea JMaldonado-Valderrama MJ Gaacutelvez-Ruiz JL Ortega-Vinuesa AMartiacuten-Rodriacuteguez Adsorption of antibody onto pluronic F68-coverednanoparticles link with surface properties Soft Matter 7 (2011) 8450 httpdxdoiorg101039c1sm05570d
[46] MJ Santander-Ortega MV Lozano-Loacutepez D Bastos-Gonzaacutelez JMPeula-Garciacutea JL Ortega-Vinuesa Novel core-shell lipid-chitosan andlipid-poloxamer nanocapsules stability by hydration forces Colloid PolymSci 288 (2010) 159ndash172 httpdxdoiorg101007s00396-009-2132-y
[47] A Torcello-Goacutemez MJ Santander-Ortega JM Peula-Garciacutea JMaldonado-Valderrama MJ Gaacutelvez-Ruiz JL Ortega-Vinuesa AMartiacuten-Rodriacuteguez Adsorption of antibody onto Pluronic F68-coverednanoparticles link with surface properties Soft Matter 7 (2011) httpdxdoiorg101039c1sm05570d
[48] JP Penaloza V Maacuterquez-Miranda M Cabana-Brunod R Reyes-Ramiacuterez FMLlancalahuen C Vilos F Maldonado-Biermann LA Velaacutesquez JA FuentesFD Gonzaacutelez-Nilo M Rodriacuteguez-Diacuteaz C Otero Intracellular trafficking andcellular uptake mechanism of PHBV nanoparticles for targeted delivery inepithelial cell lines J Nanobiotechnol 15 (2017) 1 httpdxdoiorg101186s12951-016-0241-6
[49] SP Schwendeman RB Shah BA Bailey AS Schwendeman Injectablecontrolled release depots for large molecules J Controlled Release 190 (2014)240ndash253 httpdxdoiorg101016jjconrel201405057
[50] H Wang SCG Leeuwenburgh Y Li JA Jansen The use of micro- andnanospheres as functional components for bone tissue regeneration TissueEng Part B Rev 18 (2012) 24ndash39 httpdxdoiorg101089tenteb20110184
[51] JK Vasir V Labhasetwar Biodegradable nanoparticles for cytosolic deliveryof therapeutics Adv Drug Deliv Rev 59 (2007) 718ndash728 httpdxdoiorg101016jaddr200706003
[52] B Yameen W Il Choi C Vilos A Swami J Shi OC Farokhzad Insight intonanoparticle cellular uptake and intracellular targeting J Controlled Release190 (2014) 485ndash499 httpdxdoiorg101016jjconrel201406038
[53] H Sneh-Edri D Likhtenshtein D Stepensky Intracellular targeting of PLGAnanoparticles encapsulating antigenic peptide to the endoplasmic reticulumof dendritic cells and its effect on antigen cross-presentation in vitro MolPharm 8 (2011) 1266ndash1275 httpdxdoiorg101021mp200198c
[54] MJ Santander-Ortega AB Joacutedar-Reyes N Csaba D Bastos-Gonzaacutelez JLOrtega-Vinuesa Colloidal stability of Pluronic F68-coated PLGAnanoparticles a variety of stabilisation mechanisms J Colloid Interface Sci302 (2006) 522ndash529 httpdxdoiorg101016jjcis200607031
[55] B Baumann T Jungst S Stichler S Feineis O Wiltschka M Kuhlmann MLindn J Groll Control of nanoparticle release kinetics from 3D printedhydrogel scaffolds Angew Chem ndash Int Ed 56 (2017) 4623ndash4628 httpdxdoiorg101002anie201700153
[56] S-J Lee W Zhu L Heyburn M Nowicki B Harris LG Zhang Developmentof novel 3-D printed scaffolds with core-shell nanoparticles for nerveregeneration IEEE Trans Biomed Eng 64 (2017) 408ndash418 httpdxdoiorg101109tbme20162558493
[57] DJ Hines DL Kaplan Poly(lactic-co-glycolic) acid-controlled-releasesystems experimental and modeling insights Crit Rev Ther Drug CarrierSyst 30 (2013) 257ndash276 httpdxdoiorg101615CritRevTherDrugCarrierSyst2013006475
[58] H Begam SK Nandi B Kundu A Chanda Strategies for delivering bonemorphogenetic protein for bone healing Mater Sci Eng C 70 (2016)856ndash869 httpdxdoiorg101016jmsec201609074
[59] YH Kim Y Tabata Dual-controlled release system of drugs for boneregeneration Adv Drug Deliv Rev 94 (2015) 28ndash40 httpdxdoiorg101016jaddr201506003
[60] N Rescignano L Tarpani A Romani I Bicchi S Mattioli C Emiliani L TorreJM Kenny S Martino L Latterini I Armentano In-vitro degradation of PLGAnanoparticles in aqueous medium and in stem cell cultures by monitoring thecargo fluorescence spectrum Polym Degrad Stab 134 (2016) 296ndash304httpdxdoiorg101016jpolymdegradstab201610017
[61] M Morille T Van-Thanh X Garric J Cayon J Coudane D Noeumll MCVenier-Julienne CN Montero-Menei New PLGA-P188-PLGA matrix enhancesTGF-3 release from pharmacologically active microcarriers and promoteschondrogenesis of mesenchymal stem cells J Control Release 170 (2013)99ndash110 httpdxdoiorg101016jjconrel201304017
[62] A Bohr F Wan J Kristensen M Dyas E Stride S Baldursdottiacuter MEdirisinghe M Yang Pharmaceutical microparticle engineering withelectrospraying the role of mixed solvent systems in particle formation andcharacteristics J Mater Sci Mater Med 26 (2015) 61 httpdxdoiorg101007s10856-015-5379-5
[63] A Rafati A Boussahel KM Shakesheff AG Shard CJ Roberts X Chen DJScurr S Rigby-Singleton P Whiteside MR Alexander MC Davies Chemicaland spatial analysis of protein loaded PLGA microspheres for drug deliveryapplications J Control Release 162 (2012) 321ndash329 httpdxdoiorg101016jjconrel201205008
[64] R Gaudana M Gokulgandhi V Khurana D Kwatra AK Mitra Design andevaluation of a novel nanoparticulate-based formulation encapsulating a HIPcomplex of lysozyme Pharm Dev Technol 18 (2013) 752ndash759 httpdxdoiorg103109108374502012737806
[65] S Xiong X Zhao BC Heng KW Ng JSC Loo Cellular uptake of Poly-(DL-lactide-co-glycolide) (PLGA) nanoparticles synthesized through solventemulsion evaporation and nanoprecipitation method Biotechnol J 6 (2011)501ndash508 httpdxdoiorg101002biot201000351
[66] J Panyam V Labhasetwar Dynamics of endocytosis and exocytosis of poly (DL-lactide-co-glycolide) nanoparticles in vascular smooth muscle cells PharmRes 20 (2003) 212ndash220 httpwwwncbinlmnihgovpubmed12636159
pharmaceutics
Article
Formulation Colloidal Characterization and In VitroBiological Ecrarrect of BMP-2 Loaded PLGANanoparticles for Bone Regeneration
Teresa del Castillo-Santaella 1 Inmaculada Ortega-Oller 2 Miguel Padial-Molina 2 Francisco OrsquoValle 3 Pablo Galindo-Moreno 2 Ana Beleacuten Joacutedar-Reyes 14 andJoseacute Manuel Peula-Garciacutea 15
1 Biocolloid and Fluid Physics Group Department of Applied Physics University of Granada 18071 GranadaSpain
2 Department of Oral Surgery and Implant Dentistry University of Granada 18071 Granada Spain3 Department of Pathology School of Medicine amp IBIMER University of Granada 18071 Granada Spain4 Excellence Research Unit ldquoModeling Naturerdquo (MNat) University of Granada 18071 Granada Spain5 Department of Applied Physics II University of Malaga 29071 Malaga Spain Correspondence jmpeulaumaes Tel +34-952132722
Received 20 June 2019 Accepted 31 July 2019 Published 3 August 2019$amp($amp
Abstract Nanoparticles (NPs) based on the polymer poly (lactide-co-glycolide) acid (PLGA) havebeen widely studied in developing delivery systems for drugs and therapeutic biomolecules due tothe biocompatible and biodegradable properties of the PLGA In this work a synthesis method forbone morphogenetic protein (BMP-2)-loaded PLGA NPs was developed and optimized in order tocarry out and control the release of BMP-2 based on the double-emulsion (wateroilwater WOW)solvent evaporation technique The polymeric surfactant Pluronic F68 was used in the synthesisprocedure as it is known to have an ecrarrect on the reduction of the size of the NPs the enhancement oftheir stability and the protection of the encapsulated biomolecule Spherical solid polymeric NPswere synthesized showing a reproducible multimodal size distribution with diameters between100 and 500 nm This size range appears to allow the protein to act on the cell surface and at thecytoplasm level The ecrarrect of carrying BMP-2 co-adsorbed with bovine serum albumin on the NPsurface was analyzed The colloidal properties of these systems (morphology by SEM hydrodynamicsize electrophoretic mobility temporal stability protein encapsulation and short-term release profile)were studied The ecrarrect of both BMP2-loaded NPs on the proliferation migration and osteogenicdicrarrerentiation of mesenchymal stromal cells from human alveolar bone (ABSC) was also analyzedin vitro
Keywords BMP-2 PLGA nanoparticles Pluronic F68
1 Introduction
In the context of nanomedicine tissue regeneration using colloidal micro- and nano-structureshaving unique size and surface activity has received increasing attention over recent years Many ecrarrortshave been made to improve the engineering of these nano-systems in order to reach a ldquosmartrdquo deliveryof bioactive molecules in order to optimize their therapeutic advantages and minimize harmful sideecrarrects [1] With this aim a broad spectrum of biocompatible nanocarriers has been described showingproperties suitable for dicrarrerent biological and therapeutic applications [2] Among these variedproposals polymeric nanosystems represent a major group in which poly lactic-co-glycolic acid (PLGA)is one of the most widely used due to its biocompatibility biodegradability and low cytotoxicitygaining the approval from dicrarrerent drug agencies for human use [34]
Pharmaceutics 2019 11 388 doi103390pharmaceutics11080388 wwwmdpicomjournalpharmaceutics
Pharmaceutics 2019 11 388 2 of 18
PLGA-based structures are described as micro- and nanocarriers to deliver a wide variety of activemolecules and drugs synthetic or natural molecules with hydrophilic or hydrophobic properties andbiomolecules from proteins to nucleic acids [5ndash7] PLGA micro- and nanosystems can be set up usingdicrarrerent formulation techniques with the possibility of a systemic or local distribution These systemscan be applied not only in tissue regeneration but also in very diverse therapies Anticancer drugdelivery infections inflammatory diseases or gene therapy [3] Despite this great potential certainapplications especially in protein encapsulation are hindered by problems such as an uncontrolledrelease profile and protein denaturation [8ndash11]
The water-in oil-in water (WOW) double emulsion method is an ldquoemulsion solvent evaporationrdquotechnique frequently used to encapsulate hydrophilic molecules as proteins in PLGA NPs [612]The appropriate choice of organic solvents the use of polymer-surfactant blends and the addition ofstabilizer-protective agents have proved to be key aspects for optimizing the resulting systems [911]Additionally a surface specific functionalization can be used to improve their versatility allowingthe chemical surface immobilization of dicrarrerent molecules in order to confer targeting or adhesiveproperties to these nanocarriers [13]
Within tissue engineering bone regeneration has a broad range of applications mostly in the fieldof dentistry where PLGA is suggested as a reference polymer to formulate NPs with bone-healinguses [14] The literature describes the delivery of bioactive molecules normally growth factors usingpolymeric microparticles (MPs) and NPs with PLGA as the main component [13] Among the bonemorphogenetic growth factors BMP-2 (bone morphogenetic protein 2) has been the most frequentlycited with many examples in which encapsulation or surface adsorption enables adequate entrapmenteciency and diverse release patterns [15ndash19] For proteins with a very short half-life such as BMPsbiodegradable PLGA nanosystems provide protection and optimal dosage for an adequate stimulationof cell dicrarrerentiation [2021]
Thus within this scenario in the present work we seek to optimize a nano-particulate system inorder to carry out and control the release of BMP-2 using as a starting point the synthesis procedure ofa lysozyme-loaded NP system previously described for the encapsulation of that model protein [11]Also to encapsulate BMP-2 we prepared a second system in which this protein was co-adsorbedwith bovine serum albumin onto the surface of empty NPs The size and morphology the proteinencapsulation eciency the surface characteristics and the colloidal and temporal stability werestudied to complete the physico-chemical characterization of both NP systems
The release profile of BMP-2 indicates the potential of a PLGA nanocarrier for bone regenerationand depends heavily on the polymer degradation by hydrolysis [22] However over the shortterm during which the release does not depend on this chemical degradation proper control ofrelease is necessary in order to modulate other physical processes Thus we focused our releaseexperiments on the short-term using dicrarrerent techniques to compare the two NP samples and establishthe corresponding BMP-2 release profiles Finally the biological activity (cell migration proliferationand osteogenic dicrarrerentiation) was tested in vitro using mesenchymal stromal cells (MSCs) derivedfrom alveolar bone [23]
2 Materials and Methods
21 Nanoparticle Synthesis
211 Formulation
Poly(lactide-co-glycolide) acid (PLGA 5050) ([C2H2O2]x[C3H4O2]y) x = 50 y = 50 (Resomerreg
503H (Evonik Essen Germany) 32ndash44 kDa was used as the polymer and polymeric surfactantPluronic F68 (Poloxamer 188) (Sigma-Aldrich St Louis MO USA) as the emulsifier Their structurebased on a poly(ethylene oxide)-block-poly(propylene oxide)-block-poly(ethylene oxide) is expressedas PEOa-PPOb-PEOa with a = 75 and b = 30 Human recombinant bone morphogenetic proteinrhBMP-2 (Sigma-H4791) was used as therapeutic biomolecule Water was purified in a Milli-Q
Pharmaceutics 2019 11 388 3 of 18
Academic Millipore system A double-emulsion synthesis method was used following a procedurepreviously described with slight modifications [11] In this method 100 mg of PLGA and 3 mg ofdeoxycholic acid (DC) were dissolved in a tube containing 1 mL of ethyl acetate (EA) and vortexedIn total 40 microL of a bucrarrered solution at pH 128 with or without rhBMP-2 (200 microgmL) were addedand immediately sonicated (Branson Ultrasonics 450 Analog Sonifier) for 1 min (Duty cycle dial 20Output control dial 4) with the tube surrounded by ice This primary WO emulsion was poured intoa plastic tube containing 2 mL of a bucrarrered solution (pH 12) of F68 at 1 mgmL and vortexing for30 s Then the tube surrounded by ice was sonicated at the maximum amplitude for the micro tip for1 min (Output control 7) This second WOW emulsion was poured into a glass containing 10 mL ofthe bucrarrered F68 solution and kept under magnetic stirring for 2 min The organic solvent was thenrapidly extracted by evaporation under vacuum to a final volume of 8 mL The resulting empty andBMP-2 encapsulated NP systems were named NP and NP-BMP2 respectively A detailed scheme ofthe synthesis procedure with a yield based on the PLGA component always higher than 85 is shownin Figure S1 of the Supplementary Materials
212 Cleaning and Storage
After the organic solvent evaporation the sample was centrifuged for 10 min at 20 C at 12000 rpmThe supernatant was filtered using Millipore nanofilters 01microm for measuring the free non-encapsulatedprotein The pellet was then resuspended in phosphate bucrarrer (115 mM NaH2PO4) PB to a finalvolume of 4 mL and kept refrigerated at 4 C Under these conditions the systems kept colloidalstability at least for one month
213 Protein Loading and Encapsulation Eciency
The initial protein loading was optimized for the nanoparticle formulation preserving the finalcolloidal stability after the evaporation step and taking into account the amounts shown in the literaturefor this growth factor when encapsulated inside PLGA NPs [2425] Thus we chose 2 microg as the initialtotal mass of rhBMP-2 which means a relation of 2 105 ww (rhBMP-2PLGA) The amount ofencapsulated rhBMP-2 was calculated by measuring the dicrarrerence between the initial added amountand the free non-encapsulated protein present in the supernatant after the cleaning step which wastested by a specific enzyme-linked immuno-sorbent assay following the instructions of the manufacturer(ELISA kit RAB0028 from Sigma-Aldrich St Louis MO USA) Then protein-encapsulation eciency(EE) was calculated as follows
EE =MI MF
MI 100
where MI is the initial total mass of rhBMP-2 and MF is the total mass of rhBMP-2 in theaqueous supernatant
214 Physical Protein Adsorption
Bovine serum albumin (BSA) and rhBMP-2 were coupled on the empty nanoparticle surface bya physical adsorption method The appropriate volume of an aqueous protein solution containing05 mg of BSA and 2 microg of rhBMP-2 was mixed with 5 mL of acetate bucrarrer (pH 5) containing emptyNPs with 125 mg of PLGA This provided a starting amount of proteins corresponding to 004 ww(proteinPLGA) while the mass relation between proteins was 04 ww (rhBMP-2BSA) This solutionwas incubated at room temperature for 2 h under mechanical stirring The nanoparticles were separatedfrom the bucrarrer solution by centrifugation and after the supernatants were filtered (Millipore nanofilters01 microm) they were qualitatively analyzed by gel electrophoresis while the protein quantification wasmade by a bicinchoninic acid protein assay (BCA) (Sigma-Aldrich St Louis MO USA) for BSA andthe specific ELISA for rhBMP-2 The nanoparticle pellet was resuspended in phosphate bucrarrer (pH 74)and stored at 4 C This system was named NP-BSA-BMP2
Pharmaceutics 2019 11 388 4 of 18
215 Protein Separation by Gel Electrophoresis SDS-PAGE
The protein-loaded NPs and dicrarrerent supernatants were treated at 90 C for 10 min in thefollowing bucrarrer 625 mM Tris-HCl (pH 68 at 25 C) 2 (wv) sodium dodecyl sulfate (SDS) 10glycerol 001 (wv) bromophenol blue 40 mM dithiothreitol (DTT) Samples were then separated bysize in porous 12 polyacrylamide gel (1D SDS polyacrylamide gel electrophoresis) under the ecrarrectof an electric field The electrophoresis was run under constant voltage (130 V 45 min) and the gelswere stained using a Coomassie Blue solution (01 Coomassie Brilliant Blue R-250 50 methanol and10 glacial acetic acid) and destained with the same solution lacking the dye
22 Nanoparticle Characterization Morphology Size Concentration and Electrokinetic Mobility
NPs were imaged by scanning electron microscopy (SEM) with a Zeiss SUPRA 40VP field-emissionscanning electron microscope from the Scientific Instrumentation Center of the University of Granada(CIC UGR)
The hydrodynamic size distribution of the NPs was evaluated by nanoparticle tracking analysis(NTA) with a NanoSight LM10-HS (GB) FT14 (NanoSight Amesbury UK) and an sCMOS cameraThe particle concentration according to the diameter (size distribution) was calculated as an averageof at least three independent size distributions The total concentration of NPs of each system wasdetermined in order to control the number of particles used in cell experiments The measurementconditions for all samples were 25 C a viscosity of 089 cP a measurement time of 60 s and a cameragain of 250 The camera shutter was 11 and 15 ms for the empty and BMP-loaded NPs respectivelyThe detection threshold was fixed at 5
The electrophoretic mobility of the NPs was determined using a Zetasizerreg NanoZeta ZS device(Malvern Instrument Ltd Malvern UK) working at 25 C with an He-Ne laser of 633 nm and a 173
scattering angle Each data point was taken as an average over three independent sample measurementsFor each sample the electrophoretic mobility distribution and the average electrophoretic mobility(micro-average) were determined by the technique of laser Doppler electrophoresis
23 Colloidal and Temporal Stability in Biological Media
The average hydrodynamic diameter and the polydispersity index (PDI) by dynamic lightscattering (DLS) of each NP system were measured in dicrarrerent media (phosphate bucrarrer (PB) salinephosphate bucrarrer (PBS) and cell culture medium Dulbeccorsquos modified Eaglersquos medium DMEM(Sigma)) Also data on temporal stability were gathered by repeating these analyses at dicrarrerent timesafter synthesis (0 1 and 5 days) and after 1 month under storage conditions
In vitro release experiments were conducted as follows 1 mL of each sample for each incubationtime was suspended in PBS at 37 C After the corresponding time (24 48 96 168 h) NPs wereseparated from the supernatant of released proteins by centrifugation for 10 min at 14000 rpm (10 C)The NP pellet was suspended in 1 mL of 005 M NaOH and stirred for 2 h for a complete polymerdegradation The alkaline protein solution was assayed by BCA and ELISA to quantify the unreleasedamount The protein released was calculated taking into account the total encapsulated amount Allexperiments were made in triplicate
24 Cell Interactions
For all biological in vitro studies a cell population cultured from the maxillary alveolar bonewas used This population was previously characterized and confirmed to present all characteristicsof a mesenchymal stromal cell population (MSC) [23] Cells were taken from healthy humandonors after the approval from the Ethics Committee for Human Research from the University ofGranada (424CEIH2018) Regular Dulbeccorsquos modified Eaglersquos medium (DMEM) with 1 gL glucose(DMEM-LG) (Gibco) 10 fetal bovine serum (FBS) (Sigma-Aldrich St Louis MO USA) 1100 ofnon-essential amino acid solution (NEAA) (Gibco) 001 microgmL of basic fibroblast growth factor (bFGF)
Pharmaceutics 2019 11 388 5 of 18
(PeproTech London UK) 100 UmL of penicillinstreptomycin and 025 microgmL of amphotericin Bwas used as culture medium for all experiments Cultures were maintained at 37 C in a 5 CO2atmosphere (2000 cellswell) All biological experiments were repeated in triplicate at least 3 timesper condition
241 Cell Migration
A cell-migration assay was conducted as previously described [2627] Briefly MSCs weredistributed on to three wells for each condition and allowed to grow to a cell confluency close to99 in 24-wellsplate at 3000 cellscm2 and in each well three dicrarrerent scratches were made Thencells were starved for 24 h by adding culture medium without serum A scratch was made usinga pipette tip along the diameter of the well A wash step with PBS was performed to remove thescratched cells Fresh complete culture media was added and supplemented depending on the assignedgroup (BMP-2 NP- BMP2 and NP-BSA-BMP2 at 125 25 and 5 ngmL of BMP-2) Afterwardsnine images were taken from the same area in each condition until 48 h later On these images thescraped area was measured by ImageJ software (National Institute of Health Bethesda MD USAhttprsbwebnihgovij) The reduction in the scratched area over time was measured consideringthe area at time 0 as 100 open
242 Cell Proliferation
Proliferation was evaluated by a sulphorhodamine (SRB) assay [28] The assay was conducted byseeding the cells at 1500 cellscm2 in a 96-well plate at a confluence not higher than 50 After cellattachment the dicrarrerent supplements were added (BMP-2 NP- BMP2 and NP-BSA-BMP2 at 12525 and 5 ngmL of BMP-2) and the cells were maintained in culture for up to 7 days At each timepoint the cells were washed with 1X PBS and fixed by adding ice-cold 10 trichloroacetic acid for20 min at 4 C Then the cells were washed 3 times with dH2O and dried until all time points werecollected Each well received 04 SRB in 1 acetic acid for 20 min at room temperature with gentleshaking The staining was finished by washing each well 3 times with 1 acetic acid and drying it atroom temperature for 24 h The dye was retrieved from the cells by adding 10 mM Tris Base at pH 105and gently shaking for 10 min The solution recovered was then distributed in a 96-well plate and theoptical absorbance was read at 492 nm
243 Osteogenic Dicrarrerentiation
Osteogenic dicrarrerentiation was evaluated by adding osteogenic media to the cell culture incombination with free BMP-2 NP-BMP2 and NP-BSA-BMP2 at the highest dosages used inprevious experiments Cells were seeded at 3000 cellscm2 and cultured to reach an 85 to90 confluency This was followed by the addition of induction media containing 10 mM of-glycerophosphate (Fluka 50020) 01 microM of dexamethasone (Sigma-Aldrich D2915) and 005 mMof L-ascorbic acid (Sigma-Aldrich A8960) Cell cultures were maintained for 7 days to analyzeearly activity At day 7 cells were collected in 1 mL of TRIzolreg Then RNA was extracted andconverted to cDNA Alkaline phosphatase (ALP) was then evaluated expression being calculatedrelative to glyceraldehyde-3-phosphate dehydrogenase protein (GAPDH) by the 2DDCt methodThese procedures were conducted as described elsewhere [23] Forward and reverse primer sequenceswere AGCTCATTTCCTGGTATGACAAC and TTACTCCTTGGAGGCCATGTG for GAPDH andTCCAGGGATAAAGCAGGTCTTG and CTTTCTCTTTCTCTGGCACTAAGG for ALP
244 Statistical Evaluation
Cell migration and proliferation were evaluated by ANOVA followed by Tukey multiplecomparisons test for pairwise analysis Comparison between the levels of ALP at 4 vs 7 dayswere analyzed by paired Studentrsquos t test In all cases a p value lower than 005 was established asstatistical significance
Pharmaceutics 2019 11 388 6 of 18
3 Results and Discussion
31 Nanoparticle Formulation
Double emulsion-solvent evaporation has been described as a robust and frequently used methodto produce biomolecule-loaded PLGA NPs [6121329] A formulation previously optimized byour group enabled the preservation of the biological activity of encapsulated biomolecules using aslightly aggressive organic solvent Moreover deoxycholic acid has been used in the first step of theformulation in order to improve the colloidal stability of NPs and simultaneously to obtain NP surfacesenriched with carboxylic groups improving their versatility and allowing a subsequent chemicalimmobilization of dicrarrerent specific ligands [30] By means of this improved formulation in the presentwork we developed empty nanoparticles (NPs) or nanoparticles encapsulating rhBMP-2 (NP-BMP2)A schematic description of the synthesis procedure is shown in Figure S1 of the Supplementary DataFor NP-BMP2 we achieved a protein-encapsulation eciency (EE) of 97 plusmn 2 This result is consistentwith the literature in which several authors have reported similarly high values encapsulating thisprotein inside PLGA nano- and microparticles [3132] Our formulation has several factors leading tothis very high EE value The low proteinpolymer relation in mass [33] the anity of rhBMP-2 to anunspecific interaction with hydrophobic surfaces [31] or the addition of stabilizers (poloxamer) inthe second step of the double-emulsion procedure [13] The absence of rhBMP-2 in the supernatantresulting from the centrifugation step in the cleaning process was verified by ELISA and SDS-PAGE inwhich a clear band corresponding to 14 kD of rhBMP-2 polypeptidic chains is shown for lane A inFigure 1 corresponding to NP-BMP2 The mass of protein encapsulated around 2 microg is similar to thatof dicrarrerent PLGA micro- and nanosystems described in the literature [183435] Taking into accountthe storage conditions for our samples this corresponds to 500 ngmL which represents a sucientconcentration for practical applications since this growth factor shows in vitro biological activities atvery low dosages (5ndash20 ngmL) [13]
Pharmaceutics 2019 11 x 6 of 18
31 Nanoparticle Formulation
Double emulsion-solvent evaporation has been described as a robust and frequently used method to produce biomolecule-loaded PLGA NPs [6121329] A formulation previously optimized by our group enabled the preservation of the biological activity of encapsulated biomolecules using a slightly aggressive organic solvent Moreover deoxycholic acid has been used in the first step of the formulation in order to improve the colloidal stability of NPs and simultaneously to obtain NP surfaces enriched with carboxylic groups improving their versatility and allowing a subsequent chemical immobilization of different specific ligands [30] By means of this improved formulation in the present work we developed empty nanoparticles (NPs) or nanoparticles encapsulating rhBMP-2 (NP-BMP2) A schematic description of the synthesis procedure is shown in Figure S1 of the Supplementary Data For NP-BMP2 we achieved a protein-encapsulation efficiency (EE) of 97 plusmn 2 This result is consistent with the literature in which several authors have reported similarly high values encapsulating this protein inside PLGA nano- and microparticles [3132] Our formulation has several factors leading to this very high EE value The low proteinpolymer relation in mass [33] the affinity of rhBMP-2 to an unspecific interaction with hydrophobic surfaces [31] or the addition of stabilizers (poloxamer) in the second step of the double-emulsion procedure [13] The absence of rhBMP-2 in the supernatant resulting from the centrifugation step in the cleaning process was verified by ELISA and SDS-PAGE in which a clear band corresponding to 14 kD of rhBMP-2 polypeptidic chains is shown for lane A in Figure 1 corresponding to NP-BMP2 The mass of protein encapsulated around 2 microg is similar to that of different PLGA micro- and nanosystems described in the literature [183435] Taking into account the storage conditions for our samples this corresponds to 500 ngmL which represents a sufficient concentration for practical applications since this growth factor shows in vitro biological activities at very low dosages (5ndash20 ngmL) [13]
Figure 1 SDS polyacrylamide gel electrophoresis (SDS-PAGE) analysis under reducing conditions of solid PLGA Nanoparticles (PLGA NPs) and liquid (supernatant) fractions of different NP systems Lane P Protein standards lane A NP-BMP2 (bone morphogenetic protein) lane B supernatant of NP-BMP2 after synthesis and encapsulation of rhBMP-2 lane C NP after physical adsorption of BSArhBMP-2 lane D supernatant after physical adsorption of BSA(bovine serum albumin)rhBMP-2 on NP system
On the other hand a second nanosystem resulted modifying the way in which rhBMP-2 is incorporated in the nanocarrier There are several examples of surface adsorption of different growth factors in micro- and nanoparticles [35ndash37] and surface immobilization over the encapsulation has recently been proposed as a way to modulate the later release of biomolecules This process which
Figure 1 SDS polyacrylamide gel electrophoresis (SDS-PAGE) analysis under reducing conditions ofsolid PLGA Nanoparticles (PLGA NPs) and liquid (supernatant) fractions of dicrarrerent NP systems LaneP Protein standards lane A NP-BMP2 (bone morphogenetic protein) lane B supernatant of NP-BMP2after synthesis and encapsulation of rhBMP-2 lane C NP after physical adsorption of BSArhBMP-2lane D supernatant after physical adsorption of BSA(bovine serum albumin)rhBMP-2 on NP system
On the other hand a second nanosystem resulted modifying the way in which rhBMP-2 isincorporated in the nanocarrier There are several examples of surface adsorption of dicrarrerent growthfactors in micro- and nanoparticles [35ndash37] and surface immobilization over the encapsulation has
Pharmaceutics 2019 11 388 7 of 18
recently been proposed as a way to modulate the later release of biomolecules This process whichdepends on the slow dicrarrusion of biomolecules through the polymeric matrix is consequently highlyinfluenced by the proteinndashpolymer interaction [3839] and polymer degradation [36] Thus this newfocus on the use of PLGA NPs for biomolecule delivery was explored by immobilizing the proteinrhBMP-2 on the surface of empty NPs by means of simple physical adsorption This process is knownto be governed by electrostatic and hydrophobic interactions between protein molecules and NPsurfaces [40]
For this the surface-charged groups the hydrophilicity the net charge of the protein moleculesand the characteristics of the adsorption medium are the reference parameters Thus we designed aco-adsorption experiment in which a mixture of rhBMP-2 and BSA (04 ww rhBMP-2BSA) interactsimultaneously with the PLGA NP surface Albumins are routinely used as protective proteins whengrowth factors are incorporated in PLGA NPs [1319] Moreover a surface distribution of BSA moleculescan improve the colloidal stability of NPs at physiological pH due to their net negative charge underthese conditions [41] Figure S2 from Supplementary Materials shows a scheme of the co-adsorptionprocess The adsorption eciency is higher than 95 and in SDS-PAGE from Figure 1 two bandscharacteristic of both proteins can be seen in lane C corresponding to the NP-BSA-BMP2 nanosystemHowever lane D corresponding to the run of the supernatant from the centrifugation of the nanosystemafter adsorption processes shows the absence of any protein This result is fully explained by takinginto account the pH of the medium (pH 50) near the isoelectric point of BSA where the adsorption ofthis protein onto negatively charged nanoparticles presents a maximum [4042] The immobilizationof rhBMP-2 on the negatively charged surface of NPs proves they are electrostatically favored due tothe positive net charge of this protein at acid and neutral pH
32 Nanoparticle Characterization
321 Nanoparticle Size
SEM and STEM micrographs (Figure 2) show that the samples consist of spherical particles ofdicrarrerent diameters (between 150 and 450 nm) a range similar to that found in a previous work inwhich NPs were loaded with lysozyme following a similar synthesis protocol [11] In that workthe DLS technique failed to provide a reliable size distribution Therefore the NTA technique wasdirectly used to determine the hydrodynamic size of the BMP2-loaded NPs (see NTA video in theSupplementary Material)
The size distributions for empty (NP) and BMP-loaded NPs (NP-BMP2) from NTA (Figure 3 andvideos S1 S2) were consistent with the SEM images Particles with diameters between 100 and 500 nmwere found to have the highest particle concentration at around 200 nm The loading with BMP hadan ecrarrect on the size distribution leading to more defined peaks These measurements enabled usto determine the concentration of particles in the measured sample 688 plusmn 009 108 ppmL and519 plusmn 012 108 ppmL for NP and NP-BMP2 nanosystems respectively These values were used(by taking into account the corresponding dilution) to control the number of particles added in thecell experiments
Pharmaceutics 2019 11 388 8 of 18
Pharmaceutics 2019 11 x 7 of 18
depends on the slow diffusion of biomolecules through the polymeric matrix is consequently highly influenced by the proteinndashpolymer interaction [3839] and polymer degradation [36] Thus this new focus on the use of PLGA NPs for biomolecule delivery was explored by immobilizing the protein rhBMP-2 on the surface of empty NPs by means of simple physical adsorption This process is known to be governed by electrostatic and hydrophobic interactions between protein molecules and NP surfaces [40]
For this the surface-charged groups the hydrophilicity the net charge of the protein molecules and the characteristics of the adsorption medium are the reference parameters Thus we designed a co-adsorption experiment in which a mixture of rhBMP-2 and BSA (04 ww rhBMP-2BSA) interact simultaneously with the PLGA NP surface Albumins are routinely used as protective proteins when growth factors are incorporated in PLGA NPs [1319] Moreover a surface distribution of BSA molecules can improve the colloidal stability of NPs at physiological pH due to their net negative charge under these conditions [41] Figure S2 from Supplementary Materials shows a scheme of the co-adsorption process The adsorption efficiency is higher than 95 and in SDS-PAGE from Figure 1 two bands characteristic of both proteins can be seen in lane C corresponding to the NP-BSA-BMP2 nanosystem However lane D corresponding to the run of the supernatant from the centrifugation of the nanosystem after adsorption processes shows the absence of any protein This result is fully explained by taking into account the pH of the medium (pH 50) near the isoelectric point of BSA where the adsorption of this protein onto negatively charged nanoparticles presents a maximum [4042] The immobilization of rhBMP-2 on the negatively charged surface of NPs proves they are electrostatically favored due to the positive net charge of this protein at acid and neutral pH
32 Nanoparticle Characterization
321 Nanoparticle Size
SEM and STEM micrographs (Figure 2) show that the samples consist of spherical particles of different diameters (between 150 and 450 nm) a range similar to that found in a previous work in which NPs were loaded with lysozyme following a similar synthesis protocol [11] In that work the DLS technique failed to provide a reliable size distribution Therefore the NTA technique was directly used to determine the hydrodynamic size of the BMP2-loaded NPs (see NTA video in the Supplementary Material)
Figure 2 Scanning electron microscopy (SEM) micrograph of rhBMP-2-loaded nanoparticles (NP-BMP2)
Figure 2 Scanning electron microscopy (SEM) micrograph of rhBMP-2-loaded nanoparticles(NP-BMP2)
Pharmaceutics 2019 11 x 8 of 18
The size distributions for empty (NP) and BMP-loaded NPs (NP-BMP2) from NTA (Figure 3 and videos S1 S2) were consistent with the SEM images Particles with diameters between 100 and 500 nm were found to have the highest particle concentration at around 200 nm The loading with BMP had an effect on the size distribution leading to more defined peaks These measurements enabled us to determine the concentration of particles in the measured sample 688 plusmn 009 times 108 ppmL and 519 plusmn 012 times 108 ppmL for NP and NP-BMP2 nanosystems respectively These values were used (by taking into account the corresponding dilution) to control the number of particles added in the cell experiments
Figure 3 Hydrodynamic diameter distribution of NP (circles) and NP-BMP2 (thick black line) measured at pH 70 (phosphate buffer) by nanoparticle tracking analysis (NTA)
322 Electrokinetic Mobility and Colloidal Stability
The surface charge of nanoparticles can be analyzed using an electrokinetic study by measuring the electrophoretic mobility (microe) under different conditions Figure 4 shows the microe and zeta potential values for the three nanosystems NP NP-BMP2 and NP-BSA-BMP2 at low ionic strength and different pH values The electric surface charge of NPs resides in the carboxylic groups of the uncapped PLGA and deoxycholic acid molecules These functionalized groups are additionally useful due to the possibility of a chemical surface vectorization in order to develop directed delivery nanocarriers [43] It was previously confirmed that protonation of these acidic surface groups at pH values under their pKa value was tightly correlated with a loss of surface charge and consequently a reduction (in absolute value) of the electrophoretic mobility of the colloidal system [4445] Usually when colloidal particles are coated by protein molecules the microe values change markedly compared with the same bare surfaces and are influenced by the electrical charge of the adsorbed protein molecules [4647] The electrokinetic behavior of the NP-BMP2 system remains similar to that of NP and encapsulation of rhBMP-2 does not affect the surface charge distribution A similar result was reported by drsquoAngelo et al on encapsulating different growth factors in PLGA-poloxamer blend nanoparticles in the same proportion ww of proteinpolymer [24] This may be due to the low amount of encapsulated protein and its distribution in the inner part of the NPs (far from the surface) In our system this internal distribution may be favored by the encapsulating conditions where the basic pH (pH 120) of the water phase containing rhBMP-2 allows a negative charge of these protein molecules thereby preventing their electrostatic specific interaction with acidic groups of the NPs
0 200 400 600 80000
05
10
15
20
25
30
35
40
parti
cle
conc
entra
tion
(106 p
pm
L)
D(nm)
Figure 3 Hydrodynamic diameter distribution of NP (circles) and NP-BMP2 (thick black line) measuredat pH 70 (phosphate bucrarrer) by nanoparticle tracking analysis (NTA)
322 Electrokinetic Mobility and Colloidal Stability
The surface charge of nanoparticles can be analyzed using an electrokinetic study by measuringthe electrophoretic mobility (microe) under dicrarrerent conditions Figure 4 shows the microe and zeta potentialvalues for the three nanosystems NP NP-BMP2 and NP-BSA-BMP2 at low ionic strength anddicrarrerent pH values The electric surface charge of NPs resides in the carboxylic groups of the uncappedPLGA and deoxycholic acid molecules These functionalized groups are additionally useful due to thepossibility of a chemical surface vectorization in order to develop directed delivery nanocarriers [43]It was previously confirmed that protonation of these acidic surface groups at pH values undertheir pKa value was tightly correlated with a loss of surface charge and consequently a reduction (in
Pharmaceutics 2019 11 388 9 of 18
absolute value) of the electrophoretic mobility of the colloidal system [4445] Usually when colloidalparticles are coated by protein molecules the microe values change markedly compared with the samebare surfaces and are influenced by the electrical charge of the adsorbed protein molecules [4647]The electrokinetic behavior of the NP-BMP2 system remains similar to that of NP and encapsulationof rhBMP-2 does not acrarrect the surface charge distribution A similar result was reported by drsquoAngeloet al on encapsulating dicrarrerent growth factors in PLGA-poloxamer blend nanoparticles in the sameproportion ww of proteinpolymer [24] This may be due to the low amount of encapsulated proteinand its distribution in the inner part of the NPs (far from the surface) In our system this internaldistribution may be favored by the encapsulating conditions where the basic pH (pH 120) of the waterphase containing rhBMP-2 allows a negative charge of these protein molecules thereby preventingtheir electrostatic specific interaction with acidic groups of the NPs
Pharmaceutics 2019 11 x 9 of 18
Figure 4 Electrophoretic mobility and zeta potential vs pH in buffered media of low salinity (ionic strength equal to 0002 M) for the different nanosystems (black square) NP (blue triangle) NP-BMP2 (red circle) NP-BSA-BMP2
The electrokinetic distribution for the NP-BSA-BMP2 system radically changes As previously shown the very high adsorption efficiency leads to NPs with both proteins adsorbed around their surface This situation is closely correlated with the microe values from Figure 4 Taking into account the ww relation between adsorbed proteins (250 times higher for BSA) albumin molecules modulate the behavior at pH values below their isoelectric point (pI 47) where the positive net charge of BSA masks the original surface charge of NPs and even changes their original values to positive ones This is a typical result found for this protein-covering colloidal particles [4248] At neutral and basic pH values BSA molecules have a negative net charge and the slight decrease in the absolute microe values could be due to the reduction of the negative net surface charge of NPs which may be shielded at least in a small part by the positive charge of rhBMP-2 molecules under their basic isoelectric point (pI 90)
The colloidal stability for the different nanosystems (NP NP-BMP2 and NP-BSA-BMP2) was determined by analyzing the size distributions in various media (PB PBS and DMEM) at different times after synthesis (0 1 and 5 days) Size distributions similar to the original ones were found for the two formulations NP and NP-BMP2 in all the media analyzed This result was similar to that previously found for these types of NPs encapsulating lysozyme [11] in which the combination of electrostatic and steric interactions generated by surface chemical groups of NPs confer the stability mechanism that prevents colloidal aggregation [33] The decrease of the absolute value of the zeta potential for the NP-BSA-BMP2 system as a consequence of surface protein distribution does not affect its colloidal stability This system also maintains the same size distribution in the different media It is commonly accepted that a zeta potential higher than +30 or minus30 mV will give rise to a stable colloidal system [49] and the zeta potential value for NP-BSA-BMP2 is above minus30 mV Colloidal stability in PBS and DMEM typically used media for the development of scaffold or cell interactions
Figure 4 Electrophoretic mobility and zeta potential vs pH in bucrarrered media of low salinity (ionicstrength equal to 0002 M) for the dicrarrerent nanosystems (black square) NP (blue triangle) NP-BMP2(red circle) NP-BSA-BMP2
The electrokinetic distribution for the NP-BSA-BMP2 system radically changes As previouslyshown the very high adsorption eciency leads to NPs with both proteins adsorbed around theirsurface This situation is closely correlated with the microe values from Figure 4 Taking into account theww relation between adsorbed proteins (250 times higher for BSA) albumin molecules modulate thebehavior at pH values below their isoelectric point (pI 47) where the positive net charge of BSA masksthe original surface charge of NPs and even changes their original values to positive ones This is atypical result found for this protein-covering colloidal particles [4248] At neutral and basic pH valuesBSA molecules have a negative net charge and the slight decrease in the absolute microe values could bedue to the reduction of the negative net surface charge of NPs which may be shielded at least in asmall part by the positive charge of rhBMP-2 molecules under their basic isoelectric point (pI 90)
The colloidal stability for the dicrarrerent nanosystems (NP NP-BMP2 and NP-BSA-BMP2) wasdetermined by analyzing the size distributions in various media (PB PBS and DMEM) at dicrarrerenttimes after synthesis (0 1 and 5 days) Size distributions similar to the original ones were found for
Pharmaceutics 2019 11 388 10 of 18
the two formulations NP and NP-BMP2 in all the media analyzed This result was similar to thatpreviously found for these types of NPs encapsulating lysozyme [11] in which the combination ofelectrostatic and steric interactions generated by surface chemical groups of NPs confer the stabilitymechanism that prevents colloidal aggregation [33] The decrease of the absolute value of the zetapotential for the NP-BSA-BMP2 system as a consequence of surface protein distribution does not acrarrectits colloidal stability This system also maintains the same size distribution in the dicrarrerent media It iscommonly accepted that a zeta potential higher than +30 or 30 mV will give rise to a stable colloidalsystem [49] and the zeta potential value for NP-BSA-BMP2 is above 30 mV Colloidal stability in PBSand DMEM typically used media for the development of scacrarrold or cell interactions respectivelyassures the potential use of these nanosystems for in vitro or in vivo living environments Additionallythese systems maintained their size under storage in PB at 4 C for at least 1 month (data not shown)showing this to be an adequate medium for sample storage
323 Protein Release
One of the main problems for micro- or nanosystems of PLGA drug delivery is to find theappropriate release pattern for encapsulatedattached protein molecules A wide spectrum offormulations modulates this property by the use of dicrarrerent types of synthesis processes PLGApolymers co-polymers and stabilizers [313] An adequate limitation and control in the burst releaseis critical for BMPs in order to ensure long-term continuous release that favored by the polymerdegradation provides better in vivo action in driving bone and cartilage regeneration [20] Thereforewe previously developed a dual PLGA nanosystem for controlled short-term release where proteindicrarrusion and proteinndashpolymer interaction are the main factors governing this process [11]
In the present work NP-BMP2 and NP-BSA-BMP2 nanosystems represent two dicrarrerent ways inwhich rhBMP-2 was incorporated into the nanocarrier Figure 5A shows the cumulative release of bothproteins rhBMP-2 and BSA for dicrarrerent systems as a function of time in a short-term period (7 days)The encapsulated rhBMP-2 protein reaches an amount released of around 30 of the initial encapsulatedone while adsorbed rhBMP-2 despite its surface distribution is three times lower However BSAshows released amounts up to 80 of the initial adsorbed ones In all cases error bars correspond tothe standard deviations from three independent experiments Under these conditions the growthfactor encapsulated in NP-BMP2 presents a release pattern similar to that previously found with thesame formulation but using lysozyme as the protein [11] Poloxamer in the water phase of the synthesisprocess can be key in modulating both specific and unspecific interfacial protein interactions [50]Thus the relation between proteinndashpolymer interaction and protein dicrarrusion appears to be wellbalanced preventing an excessive initial burst and simultaneously maintaining the needed proteinflux to release around a third of the encapsulated rhBMP-2 in 7 days Although an excessive initialburst has been widely reported for PLGA NPs related with protein molecules close to the surface [6]this situation did not appear for the NP-BMP2 system this being consistent with the electrokineticbehavior that did not show the presence of protein near surface The literature ocrarrers some exampleswith reduced short-term release of BMP-2 using more hydrophilic PLGA-PEG co-polymers [16] or adicrarrerent synthesis process [25]
The release performance for the NP-BSA-BMP2 system also shown in Figure 5A presents notabledicrarrerences The electrokinetic profile has previously justified the surface location of BSA and rhBMP-2on the surface which could lead to a fast release of both proteins However results from Figure 5ABshow this trend only for the BSA protein that is released from NPs with about 20 of the initial amountremaining after seven days However up to 90 of the initial load of rhBMP-2 protein unlike BSAremains attached to the surface The NP surface with hydrophilic groups form poloxamer moleculesand a negative charge due to the abundant presence of carboxylic groups (end-groups of PLGA anddeoxycholic acid molecules) favor a desorption process for BSA whose molecules have a negativecharge under release conditions (physiological pH) This agrees with the results of other authors whoeven after encapsulating BSA in PLGA-poloxamer blend NPs achieved a fast burst release of above
Pharmaceutics 2019 11 388 11 of 18
40 to 50 of the initial protein amount [33] Moreover the co-encapsulation of albumins with growthfactors could strongly acrarrect its release profile causing an initial burst [2124] Otherwise the specificelectrostatic attraction between positive rhBMP-2 molecules and negative surface groups slows downthe short time release of this protein This result is in agreement with the low release of adsorbedBMP previously found using PLGA micro- and nanoparticles with uncapped acid end groups [3851]Thus the combination of dicrarrerent methods for trapping BMP-2 into and around NPs shows up thepossibility of attaining a properly controlled release balancing the interactions between polymersstabilizers and protein
Pharmaceutics 2019 11 x 10 of 18
respectively assures the potential use of these nanosystems for in vitro or in vivo living
environments Additionally these systems maintained their size under storage in PB at 4 degC for at
least 1 month (data not shown) showing this to be an adequate medium for sample storage
323 Protein Release
One of the main problems for micro- or nanosystems of PLGA drug delivery is to find the
appropriate release pattern for encapsulatedattached protein molecules A wide spectrum of
formulations modulates this property by the use of different types of synthesis processes PLGA
polymers co-polymers and stabilizers [313] An adequate limitation and control in the burst release
is critical for BMPs in order to ensure long-term continuous release that favored by the polymer
degradation provides better in vivo action in driving bone and cartilage regeneration [20] Therefore
we previously developed a dual PLGA nanosystem for controlled short-term release where protein
diffusion and proteinndashpolymer interaction are the main factors governing this process [11]
In the present work NP-BMP2 and NP-BSA-BMP2 nanosystems represent two different ways
in which rhBMP-2 was incorporated into the nanocarrier Figure 5A shows the cumulative release of
both proteins rhBMP-2 and BSA for different systems as a function of time in a short-term period (7
days) The encapsulated rhBMP-2 protein reaches an amount released of around 30 of the initial
encapsulated one while adsorbed rhBMP-2 despite its surface distribution is three times lower
However BSA shows released amounts up to 80 of the initial adsorbed ones In all cases error bars
correspond to the standard deviations from three independent experiments Under these conditions
the growth factor encapsulated in NP-BMP2 presents a release pattern similar to that previously
found with the same formulation but using lysozyme as the protein [11] Poloxamer in the water
phase of the synthesis process can be key in modulating both specific and unspecific interfacial
protein interactions [50] Thus the relation between proteinndashpolymer interaction and protein
diffusion appears to be well balanced preventing an excessive initial burst and simultaneously
maintaining the needed protein flux to release around a third of the encapsulated rhBMP-2 in 7 days
Although an excessive initial burst has been widely reported for PLGA NPs related with protein
molecules close to the surface [6] this situation did not appear for the NP-BMP2 system this being
consistent with the electrokinetic behavior that did not show the presence of protein near surface
The literature offers some examples with reduced short-term release of BMP-2 using more
hydrophilic PLGA-PEG co-polymers [16] or a different synthesis process [25]
(A) (B)
Figure 5 (A) Cumulative release of rhBMP-2 for NP-BMP2 (black square) and NP-BSA-BMP2 (red
circle) systems and cumulative release of BSA for NP-BSA-BMP2 (blue triangle) system incubated
for different times at 37 degC in saline phosphate buffer (pH 74) (B) SDS-PAGE analysis under reducing
0 20 40 60 80 100 120 140 160 180
0
20
40
60
80
100
Cum
ulat
ive
rele
ase
()
Time (hours)
Figure 5 (A) Cumulative release of rhBMP-2 for NP-BMP2 (black square) and NP-BSA-BMP2 (redcircle) systems and cumulative release of BSA for NP-BSA-BMP2 (blue triangle) system incubated fordicrarrerent times at 37 C in saline phosphate bucrarrer (pH 74) (B) SDS-PAGE analysis under reducingconditions of solid fraction of NP-BSA-BMP2 after release at dicrarrerent times where the number of eachlane corresponds to the time in hours
33 Biological Activity and Interactions
331 Cell Migration
Cell migration is the first and necessary step in tissue regeneration [52] Thus a regenerativeagent must accelerate cell migration or at least not interfere with it In the present study we found nodicrarrerences between the groups doses and control in terms of closure of a scratched area (ANOVAwith Tukey multiple comparisons test) (Figure 6) In contrast to our findings previously publisheddata suggests a positive ecrarrect of BMP-2 on cell migration [5354] However in those studies the dosesapplied and the cell types were dicrarrerent than in the current experiments We used lower doses ofBMP-2 in order to test whether even at low dosages BMP-2 could still provide benefits if protectedin a nanoparticle system As mentioned we demonstrated no negative ecrarrect of the system on cellmigration Our results nonetheless support the idea that BMP-2 activity is mediated by the activation ofthe phosphoinositide 3-kinase (PI3K) pathway a common group of signaling molecules that participatein several process with BMP-2 and other molecules [2654] It should also be mentioned that thetimeframe of a migration assay is short Thus the potential advantages of a controlled-release systemas the one under study might be limited That is the release of BMP-2 from the nanoparticles asdemonstrated in Figure 5 is limited to the first 48 h Thus a sustained positive ecrarrect on migrationactivity over time could be hypothesized
Pharmaceutics 2019 11 388 12 of 18Pharmaceutics 2019 11 x 12 of 18
Figure 6 Migration assay Percentage of scratched area closure at 24 and 48 h on different groups and doses
332 Cell Proliferation
Proliferation is another of the cell activities required for tissue regeneration However this property must be balanced with both migration and differentiation and not all three characteristics increase at the same time and with the same ratios [55] In fact reportedly when a dose of BMP-2 induces higher proliferation it decreases differentiation [56] This property has been extensively analyzed but discrepancies can still be detected in the literature Therefore Kim et al analyzed different doses of BMP-2 and its effect on cell proliferation and apoptosis It was confirmed in vitro that high doses but still lower than those used clinically reduce cell proliferation and increase apoptosis [57] This should be avoided We have found that although free BMP-2 does not induce higher proliferation than the control at any of the doses applied nor time points (ANOVA with Tukey multiple comparisons test) the same amount of BMP-2 encapsulated or adsorbed onto PLGA nanoparticles boosts proliferation this being statistically significant when using a dose of 25 ngmL or higher (ANOVA with Tukey multiple comparisons test) (Figure 7) These dosages are still lower than those suggested in previous studies Apart from that difference a positive effect on proliferation was still achieved Moreover following the release pattern from Figure 5 more BMP-2 is expected to be released over time beyond the 7-day time frame Thus a sustained induction effect could be expected as well until full confluency of the cell culture
0
10
20
30
40
50
60
70
80
90
100
0 h 24 h 48 h
o
f scr
atch
ed a
rea
Time point
Control
BMP2-125 ngmL
BMP2-25 ngmL
BMP2-5 ngmL
NP-BMP2-125 ngmL
NP-BMP2-25 ngmL
NP-BMP2-5 ngmL
NP-BSA-BMP2-125 ngmL
NP-BSA-BMP2-25 ngmL
NP-BSA-BMP2-5 ngmL
Figure 6 Migration assay Percentage of scratched area closure at 24 and 48 h on dicrarrerent groupsand doses
332 Cell Proliferation
Proliferation is another of the cell activities required for tissue regeneration However this propertymust be balanced with both migration and dicrarrerentiation and not all three characteristics increaseat the same time and with the same ratios [55] In fact reportedly when a dose of BMP-2 induceshigher proliferation it decreases dicrarrerentiation [56] This property has been extensively analyzed butdiscrepancies can still be detected in the literature Therefore Kim et al analyzed dicrarrerent doses ofBMP-2 and its ecrarrect on cell proliferation and apoptosis It was confirmed in vitro that high doses butstill lower than those used clinically reduce cell proliferation and increase apoptosis [57] This shouldbe avoided We have found that although free BMP-2 does not induce higher proliferation than thecontrol at any of the doses applied nor time points (ANOVA with Tukey multiple comparisons test)the same amount of BMP-2 encapsulated or adsorbed onto PLGA nanoparticles boosts proliferationthis being statistically significant when using a dose of 25 ngmL or higher (ANOVA with Tukeymultiple comparisons test) (Figure 7) These dosages are still lower than those suggested in previousstudies Apart from that dicrarrerence a positive ecrarrect on proliferation was still achieved Moreoverfollowing the release pattern from Figure 5 more BMP-2 is expected to be released over time beyondthe 7-day time frame Thus a sustained induction ecrarrect could be expected as well until full confluencyof the cell culture
Pharmaceutics 2019 11 388 13 of 18Pharmaceutics 2019 11 x 13 of 18
Figure 7 Proliferation of human mesenchymal stromal cells (MSCs) as measured by sulphorhodamine (SRB) absorbance Results were normalized to T0 in each group
333 Osteogenic Differentiation
It has been confirmed that cell differentiation induced by BMP-2 needs the presence of permissive osteoinductive components Particularly β-glycerophosphate has been shown to exert a synergistic effect with BMP-2 in inducing cell differentiation [56] Thus to test for osteogenic differentiation we analyzed the expression of ALP mRNA Maximum ALP activity was found to occur 10 days after stimulation with PLGA-based microparticles containing BMP-2 in co-encapsulation with human serum albumin [16] Although other tests could have been used to reinforce our findings ALP is known to modulate the deposition of mineralized nodules thus indicating osteoblastic activity For all of this we supplemented the differentiation media with β-glycerophosphate and either free BMP-2 NP-BMP2 or NP-BSA-BMP2 for 4 and 7 days so that we could capture the early dynamics of the expression of the gene In our study we identified an increase in the expression of ALP in all groups from day 4 to day 7 (Figure 8) Although ALP at day 7 in the BMP-2 group appears to be higher than for the other two groups the change did not prove significant In fact differences between groups were not statistically significant within any time period Noteworthy though the increase was not significant within the BMP-2 group (p = 0141 Studentrsquos t test) but it was significant within the other two groups (p = 0025 and p = 0003 NP-BMP2 and NP-BSA-BMP2 groups respectively) This again could be taken as a confirmation of the sustained release of the protein from the nanoparticle system beyond the earlier time points
This and both the migration and proliferation studies described below lead us to confirm that the system proposed can maintain a proper release of BMP-2 over time sustaining a positive effect on cell migration and proliferation with initial reduced doses of BMP-2 The fact that the excessive initial burst is prevented is important for the application of this nanotechnology in bone regeneration as in dentistry In this way the negative effects of initial high doses of BMP-2 are avoided at the same time as the molecule is protected from denaturalization inside the NP Thus the regenerator effects are maintained over time
T0 T1 T2 T3 T4 T6 T70
2
4
6
Time point
Nor
mal
ized
Abs
orba
nce
NP-BSA-BMP2-125 ngmL
NP-BSA-BMP2-25 ngmL
NP-BSA-BMP2-5 ngmL
NP-BMP2-125 ngmL
NP-BMP2-25 ngmL
NP-BMP2-5 ngmL
BMP2-125 ngmL
BMP2-25 ngmL
BMP2-5 ngmL
Control
Figure 7 Proliferation of human mesenchymal stromal cells (MSCs) as measured by sulphorhodamine(SRB) absorbance Results were normalized to T0 in each group
333 Osteogenic Dicrarrerentiation
It has been confirmed that cell dicrarrerentiation induced by BMP-2 needs the presence of permissiveosteoinductive components Particularly -glycerophosphate has been shown to exert a synergisticecrarrect with BMP-2 in inducing cell dicrarrerentiation [56] Thus to test for osteogenic dicrarrerentiation weanalyzed the expression of ALP mRNA Maximum ALP activity was found to occur 10 days afterstimulation with PLGA-based microparticles containing BMP-2 in co-encapsulation with human serumalbumin [16] Although other tests could have been used to reinforce our findings ALP is known tomodulate the deposition of mineralized nodules thus indicating osteoblastic activity For all of this wesupplemented the dicrarrerentiation media with -glycerophosphate and either free BMP-2 NP-BMP2 orNP-BSA-BMP2 for 4 and 7 days so that we could capture the early dynamics of the expression of thegene In our study we identified an increase in the expression of ALP in all groups from day 4 to day 7(Figure 8) Although ALP at day 7 in the BMP-2 group appears to be higher than for the other twogroups the change did not prove significant In fact dicrarrerences between groups were not statisticallysignificant within any time period Noteworthy though the increase was not significant within theBMP-2 group (p = 0141 Studentrsquos t test) but it was significant within the other two groups (p = 0025and p = 0003 NP-BMP2 and NP-BSA-BMP2 groups respectively) This again could be taken as aconfirmation of the sustained release of the protein from the nanoparticle system beyond the earliertime points
This and both the migration and proliferation studies described below lead us to confirm that thesystem proposed can maintain a proper release of BMP-2 over time sustaining a positive ecrarrect on cellmigration and proliferation with initial reduced doses of BMP-2 The fact that the excessive initialburst is prevented is important for the application of this nanotechnology in bone regeneration asin dentistry In this way the negative ecrarrects of initial high doses of BMP-2 are avoided at the sametime as the molecule is protected from denaturalization inside the NP Thus the regenerator ecrarrects aremaintained over time
Pharmaceutics 2019 11 388 14 of 18
14 of 18
4 Conclusions
In this work a delivery PLGA-nanosystem previously developed for model proteins was chosen as the reference system to carry and deliver the growth factor BMP-2 This NP system with a dual size distribution was developed following a double-emulsion formulation in which the process and the components used were optimized to reach the appropriate colloidal and biological behavior Encapsulation and adsorption are two different processes to load BMP-2 in PLGA NPs Both were tested to elucidate the factors controlling them and their influence in the physico-chemical and biological properties of nanosystems We verified that proteinndashpolymer specific interactions have a major role in the way that protein molecules are carried and delivered from NPs In vitro experiments showed that BMP-2-loaded PLGA NPs are the nanocarriers with the best release profile over the short-term without an initial burst and with moderate and sustained release of active protein before the onset of polymer degradation Therefore the biological activity is positive with no negative interaction with migration or proliferation but rather the induction of cell differentiation through the expression of ALP
Supplementary Materials The following are available online at wwwmdpicomxxxs1 Figure S1 Scheme of the formulation of NP-BMP2 Figure S2 Scheme of the protein adsorption process for NP-BSA-BMP2 Video S1 NTA experiments for NP-BMP2 Video S2 NTA experiments for empty NPs
Author Contributions Conceptualization JMP-G and PG-M methodology JMP-G ABJ-R and MP-M investigation TdC-S IO-O JMP-G ABJ-R and MP-M resources ABJ-R PG-M FO-R writingmdashoriginal draft preparation JMP-G and MP-M writingmdashreview and editing JMP-G MP-M ABJ-R TdC-S supervision JMP-G PG-M and FO-R funding acquisition ABJ-R and PG-M
Funding This research was funded by the Consejeriacutea de Economiacutea Innovacioacuten Ciencia y Empleo de la Junta de Andaluciacutea (Spain) through research groups FQM-115 and CTS-1028 by the following research project MAT2013-43922-RmdashEuropean FEDER support includedmdash(MICINN Spain) and by MIS Ibeacuterica SL
Acknowledgments The authors wish to express their appreciation for the technical support to D Dariacuteo Abril-Garciacutea
Conflicts of Interest The authors declare no conflict of interest
Figure 8 Relative fold change in the expression of ALP mRNA (control group BMP2 at 4 days) =Statistical significance of the comparison over time (p = 0025 and p = 0003 Studentrsquos t test NP-BMP2and NP-BSA-BMP2 groups)
4 Conclusions
In this work a delivery PLGA-nanosystem previously developed for model proteins was chosenas the reference system to carry and deliver the growth factor BMP-2 This NP system with a dualsize distribution was developed following a double-emulsion formulation in which the processand the components used were optimized to reach the appropriate colloidal and biological behaviorEncapsulation and adsorption are two dicrarrerent processes to load BMP-2 in PLGA NPs Both were testedto elucidate the factors controlling them and their influence in the physico-chemical and biologicalproperties of nanosystems We verified that proteinndashpolymer specific interactions have a major role inthe way that protein molecules are carried and delivered from NPs In vitro experiments showed thatBMP-2-loaded PLGA NPs are the nanocarriers with the best release profile over the short-term withoutan initial burst and with moderate and sustained release of active protein before the onset of polymerdegradation Therefore the biological activity is positive with no negative interaction with migrationor proliferation but rather the induction of cell dicrarrerentiation through the expression of ALP
Supplementary Materials The following are available online at httpwwwmdpicom1999-4923118388s1Figure S1 Scheme of the formulation of NP-BMP2 Figure S2 Scheme of the protein adsorption process forNP-BSA-BMP2 Video S1 NTA experiments for NP-BMP2 Video S2 NTA experiments for empty NPs
Author Contributions Conceptualization JMP-G and PG-M methodology JMP-G ABJ-R andMP-M investigation TdC-S IO-O JMP-G ABJ-R and MP-M resources ABJ-R PG-M FO-Rwritingmdashoriginal draft preparation JMP-G and MP-M writingmdashreview and editing JMP-G MP-MABJ-R TdC-S supervision JMP-G PG-M and FO-R funding acquisition ABJ-R and PG-M
Funding This research was funded by the Consejeriacutea de Economiacutea Innovacioacuten Ciencia y Empleo de la Juntade Andaluciacutea (Spain) through research groups FQM-115 and CTS-1028 by the following research projectMAT2013-43922-RmdashEuropean FEDER support includedmdash(MICINN Spain) and by MIS Ibeacuterica SL
Acknowledgments The authors wish to express their appreciation for the technical support to D DariacuteoAbril-Garciacutea
Conflicts of Interest The authors declare no conflict of interest
Pharmaceutics 2019 11 388 15 of 18
References
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2 Kumar B Jalodia K Kumar P Gautam HK Recent advances in nanoparticle-mediated drug delivery JDrug Deliv Sci Technol 2017 41 260ndash268 [CrossRef]
3 Mir M Ahmed N Rehman AUR Recent applications of PLGA based nanostructures in drug deliveryColloids Surf B Biointerfaces 2017 159 217ndash231 [CrossRef] [PubMed]
4 Jana S Jana S Natural polymeric biodegradable nanoblend for macromolecules delivery In RecentDevelopments in Polymer Macro Micro and Nano Blends Woodhead Publishing Cambridge UK 2017pp 289ndash312 ISBN 9780081004081
5 Danhier F Ansorena E Silva JM Coco R Le Breton A Preacuteat V PLGA-based nanoparticles Anoverview of biomedical applications J Control Release 2012 161 505ndash522 [CrossRef] [PubMed]
6 Ding D Zhu Q Recent advances of PLGA micronanoparticles for the delivery of biomacromoleculartherapeutics Mater Sci Eng C 2018 92 1041ndash1060 [CrossRef] [PubMed]
7 Arias JL Unciti-Broceta JD Maceira J del Castillo T Hernaacutendez-Quero J Magez SSoriano M Garciacutea-Salcedo JA Nanobody conjugated PLGA nanoparticles for active targeting of AfricanTrypanosomiasis J Control Release 2015 197 190ndash198 [CrossRef]
8 Giteau A Venier-Julienne MC Aubert-Poueumlssel A Benoit JP How to achieve sustained and completeprotein release from PLGA-based microparticles Int J Pharm 2008 350 14ndash26 [CrossRef]
9 Fredenberg S Wahlgren M Reslow M Axelsson A The mechanisms of drug release inpoly(lactic-co-glycolic acid)-based drug delivery systemsmdashA review Int J Pharm 2011 415 34ndash52[CrossRef]
10 White LJ Kirby GTS Cox HC Qodratnama R Qutachi O Rose FRAJ Shakeshecrarr KM Acceleratingprotein release from microparticles for regenerative medicine applications Mater Sci Eng C 2013 332578ndash2583 [CrossRef]
11 Ortega-Oller I del Castillo-Santaella T Padial-Molina M Galindo-Moreno P Joacutedar-Reyes ABPeula-Garciacutea JM Dual delivery nanosystem for biomolecules Formulation characterization and in vitrorelease Colloids Surf B Biointerfaces 2017 159 586ndash595 [CrossRef]
12 McClements DJ Encapsulation protection and delivery of bioactive proteins and peptides usingnanoparticle and microparticle systems A review Adv Colloid Interface Sci 2018 253 1ndash22 [CrossRef]
13 Ortega-Oller I Padial-Molina M Galindo-Moreno P OrsquoValle F Joacutedar-Reyes AB Peula-Garciacutea JMBone Regeneration from PLGA Micro-Nanoparticles BioMed Res Int 2015 2015 1ndash18 [CrossRef] [PubMed]
14 Bapat RA Joshi CP Bapat P Chaubal TV Pandurangappa R Jnanendrappa N Gorain B Khurana SKesharwani P The use of nanoparticles as biomaterials in dentistry Drug Discov Today 2019 24 85ndash98[CrossRef]
15 Ji Y Xu GP Zhang ZP Xia JJ Yan JL Pan SH BMP-2PLGA delayed-release microspheres compositegraft selection of bone particulate diameters and prevention of aseptic inflammation for bone tissueengineering Ann BioMed Eng 2010 38 632ndash639 [CrossRef] [PubMed]
16 Kirby GTS White LJ Rahman CV Cox HC Qutachi O Rose FRAJ Hutmacher DWShakeshecrarr KM Woodrucrarr MA PLGA-Based Microparticles for the Sustained Release of BMP-2 Polymers2011 3 571ndash586 [CrossRef]
17 Qutachi O Shakeshecrarr KM Buttery LDK Delivery of definable number of drug or growth factor loadedpoly(dl-lactic acid-co-glycolic acid) microparticles within human embryonic stem cell derived aggregates JControl Release 2013 168 18ndash27 [CrossRef] [PubMed]
18 Wang Y Wei Y Zhang X Xu M Liu F Ma Q Cai Q Deng X PLGAPDLLA core-shell submicronspheres sequential release system Preparation characterization and promotion of bone regeneration in vitroand in vivo Chem Eng J 2015 273 490ndash501 [CrossRef]
Pharmaceutics 2019 11 388 16 of 18
19 Zhang H-X Zhang X-P Xiao G-Y Hou -Y Cheng L Si M Wang S-S Li Y-H Nie L In vitroand in vivo evaluation of calcium phosphate composite scacrarrolds containing BMP-VEGF loaded PLGAmicrospheres for the treatment of avascular necrosis of the femoral head Mater Sci Eng C 2016 60 298ndash307[CrossRef]
20 Begam H Nandi SK Kundu B Chanda A Strategies for delivering bone morphogenetic protein forbone healing Mater Sci Eng C 2017 70 856ndash869 [CrossRef]
21 Balmayor ER Feichtinger GA Azevedo HS Van Griensven M Reis RL Starch-poly--caprolactonemicroparticles reduce the needed amount of BMP-2 Clin Orthop Relat Res 2009 467 3138ndash3148 [CrossRef]
22 Xu Y Kim CS Saylor DM Koo D Polymer degradation and drug delivery in PLGA-based drugndashpolymerapplications A review of experiments and theories J BioMed Mater Res Part B Appl Biomater 2017 1051692ndash1716 [CrossRef] [PubMed]
23 Padial-Molina M de Buitrago JG Sainz-Urruela R Abril-Garcia D Anderson P OrsquoValle FGalindo-Moreno P Expression of Musashi-1 during osteogenic dicrarrerentiation of oral MSC An in vitro studyInt J Mol Sci 2019 20 2171 [CrossRef] [PubMed]
24 DrsquoAngelo I Garcia-Fuentes M Parajoacute Y Welle A Vaacutentus T Horvaacuteth A Boumlkoumlnyi G Keacuteri GAlonso MJ Nanoparticles based on PLGApoloxamer blends for the delivery of proangiogenic growthfactors Mol Pharm 2010 7 1724ndash1733 [CrossRef] [PubMed]
25 Chang H-C Yang C Feng F Lin F-H Wang C-H Chang P-C Bone morphogeneticprotein-2 loaded poly(DL-lactide-co-glycolide) microspheres enhance osteogenic potential ofgelatinhydroxyapatite-tricalcium phosphate cryogel composite for alveolar ridge augmentation JFormos Med Assoc 2017 116 973ndash981 [CrossRef] [PubMed]
26 Padial-Molina M Volk SL Rios HF Periostin increases migration and proliferation of humanperiodontal ligament fibroblasts challenged by tumor necrosis factor -crarr and Porphyromonas gingivalislipopolysaccharides J Periodontal Res 2014 49 405ndash414 [CrossRef] [PubMed]
27 Liang C-C Park AY Guan J-L In vitro scratch assay A convenient and inexpensive method for analysisof cell migration in vitro Nat Protoc 2007 2 329ndash333 [CrossRef]
28 Houghton P Fang R Techatanawat I Steventon G Hylands PJ Lee CC The sulphorhodamine (SRB)assay and other approaches to testing plant extracts and derived compounds for activities related to reputedanticancer activity Methods 2007 42 377ndash387 [CrossRef]
29 Iqbal M Zafar N Fessi H Elaissari A Double emulsion solvent evaporation techniques used for drugencapsulation Int J Pharm 2015 496 173ndash190 [CrossRef]
30 Saacutenchez-Moreno P Ortega-Vinuesa JL Boulaiz H Marchal JA Peula-Garciacutea JM Synthesis andcharacterization of lipid immuno-nanocapsules for directed drug delivery Selective antitumor activityagainst HER2 positive breast-cancer cells Biomacromolecules 2013 14 4248ndash4259 [CrossRef]
31 Lochmann A Nitzsche H von Einem S Schwarz E Maumlder K The influence of covalently linked andfree polyethylene glycol on the structural and release properties of rhBMP-2 loaded microspheres J ControlRelease 2010 147 92ndash100 [CrossRef]
32 Kempen DHR Lu L Hecrarreran TE Creemers LB Maran A Classic KL Dhert WJA Yaszemski MJRetention of in vitro and in vivo BMP-2 bioactivities in sustained delivery vehicles for bone tissue engineeringBiomaterials 2008 29 3245ndash3252 [CrossRef]
33 Santander-Ortega MJ Csaba N Gonzaacutelez L Bastos-Gonzaacutelez D Ortega-Vinuesa JL Alonso MJProtein-loaded PLGAndashPEO blend nanoparticles Encapsulation release and degradation characteristicsColloid Polym Sci 2010 288 141ndash150 [CrossRef]
34 Chung YI Ahn KM Jeon SH Lee SY Lee JH Tae G Enhanced bone regeneration with BMP-2loaded functional nanoparticle-hydrogel complex J Control Release 2007 121 91ndash99 [CrossRef]
35 La W-G Kang S-W Yang HS Bhang SH Lee SH Park J-H Kim B-S The Ecacy of BoneMorphogenetic Protein-2 Depends on Its Mode of Delivery Artif Organs 2010 34 1150ndash1153 [CrossRef]
36 Fu Y Du L Wang Q Liao W Jin Y Dong A Chen C Li Z In vitro sustained release of recombinanthuman bone morphogenetic protein-2 microspheres embedded in thermosensitive hydrogels Die Pharm2012 67 299ndash303 [CrossRef]
Pharmaceutics 2019 11 388 17 of 18
37 Rahman CV Ben-David D Dhillon A Kuhn G Gould TWA Muumlller R Rose FRAJ Shakeshecrarr KMLivne E Controlled release of BMP-2 from a sintered polymer scacrarrold enhances bone repair in a mousecalvarial defect model J Tissue Eng Regen Med 2014 8 59ndash66 [CrossRef]
38 Pakulska MM Elliott Donaghue I Obermeyer JM Tuladhar a McLaughlin CK Shendruk TNShoichet MS Encapsulation-free controlled release Electrostatic adsorption eliminates the need for proteinencapsulation in PLGA nanoparticles Sci Adv 2016 2 e1600519 [CrossRef]
39 Fu C Yang X Tan S Song L Enhancing Cell Proliferation and Osteogenic Dicrarrerentiation of MC3T3-E1Pre-osteoblasts by BMP-2 Delivery in Graphene Oxide-Incorporated PLGAHA Biodegradable MicrocarriersSci Rep 2017 7 12549 [CrossRef]
40 Peula JM de las Nieves FJ Adsorption of monomeric bovine serum albumin on sulfonated polystyrenemodel colloids 1 Adsorption isotherms and ecrarrect of the surface charge density Colloids Surf A PhysicochemEng Asp 1993 77 199ndash208 [CrossRef]
41 Peula JM de las Nieves FJ Adsorption of monomeric bovine serum albumin on sulfonated polystyrenemodel colloids 3 Colloidal stability of latexmdashProtein complexes Colloids Surf A Physicochem Eng Asp1994 90 55ndash62 [CrossRef]
42 Peula JM Hidalgo-Alvarez R De Las Nieves FJ Coadsorption of IgG and BSA onto sulfonated polystyrenelatex I Sequential and competitive coadsorption isotherms J Biomater Sci Polym Ed 1996 7 231ndash240[CrossRef]
43 Siafaka PI Uumlstuumlndag Okur N Karavas E Bikiaris DN Surface modified multifunctional and stimuliresponsive nanoparticles for drug targeting Current status and uses Int J Mol Sci 2016 17 1440[CrossRef]
44 Peula-Garciacutea JM Hidalgo-Alvarez R De Las Nieves FJ Colloid stability and electrokinetic characterizationof polymer colloids prepared by dicrarrerent methods Colloids Surf A Physicochem Eng Asp 1997 127 19ndash24[CrossRef]
45 Santander-Ortega MJ Lozano-Loacutepez MV Bastos-Gonzaacutelez D Peula-Garciacutea JM Ortega-Vinuesa JLNovel core-shell lipid-chitosan and lipid-poloxamer nanocapsules Stability by hydration forces ColloidPolym Sci 2010 288 159ndash172 [CrossRef]
46 Peula-Garcia JM Hidaldo-Alvarez R De las Nieves FJ Protein co-adsorption on dicrarrerent polystyrenelatexes Electrokinetic characterization and colloidal stability Colloid Polym Sci 1997 275 198ndash202[CrossRef]
47 Santander-Ortega MJ Bastos-Gonzaacutelez D Ortega-Vinuesa JL Electrophoretic mobility and colloidalstability of PLGA particles coated with IgG Colloids Surf B Biointerfaces 2007 60 80ndash88 [CrossRef]
48 Peula JM Callejas J de las NIeves FJ Adsorption of Monomeric Bovine Serum Albumin on SulfonatedPolystyrene Model Colloids II Electrokinetic Characterization of Latex-Protein Complexes In SurfaceProperties of Biomaterials Butterworth and Heinemann Oxford UK 1994 pp 61ndash69
49 Sun D Ecrarrect of Zeta Potential and Particle Size on the Stability of SiO2 Nanospheres as Carrier for UltrasoundImaging Contrast Agents Int J Electrochem Sci 2016 8520ndash8529 [CrossRef]
50 del Castillo-Santaella T Peula-Garciacutea JM Maldonado-Valderrama J Joacutedar-Reyes AB Interaction ofsurfactant and protein at the OW interface and its ecrarrect on colloidal and biological properties of polymericnanocarriers Colloids Surf B Biointerfaces 2019 173 295ndash302 [CrossRef]
51 Schrier JA DeLuca PP Porous bone morphogenetic protein-2 microspheres Polymer binding and in vitrorelease AAPS PharmSciTech 2001 2 66ndash72 [CrossRef]
52 Padial-Molina M OrsquoValle F Lanis A Mesa F Dohan Ehrenfest DM Wang H-L Galindo-Moreno PClinical application of mesenchymal stem cells and novel supportive therapies for oral bone regenerationBioMed Res Int 2015 2015 [CrossRef]
53 Inai K Norris RA Hocrarrman S Markwald RR Sugi Y BMP-2 induces cell migration and periostinexpression during atrioventricular valvulogenesis Dev Biol 2008 315 383ndash396 [CrossRef]
54 Gamell C Osses N Bartrons R Ruumlckle T Camps M Rosa JL Ventura F Imamura T BMP2 inductionof actin cytoskeleton reorganization and cell migration requires PI3-kinase and Cdc42 activity J Cell Sci2008 121 3960ndash3970 [CrossRef]
55 Friedrichs M Wirsdoumlerfer F Floheacute SB Schneider S Wuelling M Vortkamp A BMP signaling balancesproliferation and dicrarrerentiation of muscle satellite cell descendants BMC Cell Biol 2011 12 26 [CrossRef]
Pharmaceutics 2019 11 388 18 of 18
56 Hrubi E Imre L Robaszkiewicz A Viraacuteg L Kereacutenyi F Nagy K Varga G Jenei A Hegeduumls CDiverse ecrarrect of BMP-2 homodimer on mesenchymal progenitors of dicrarrerent origin Hum Cell 2018 31139ndash148 [CrossRef]
57 Kim HKW Oxendine I Kamiya N High-concentration of BMP2 reduces cell proliferation and increasesapoptosis via DKK1 and SOST in human primary periosteal cells Bone 2013 54 141ndash150 [CrossRef]
copy 2019 by the authors Licensee MDPI Basel Switzerland This article is an open accessarticle distributed under the terms and conditions of the Creative Commons Attribution(CC BY) license (httpcreativecommonsorglicensesby40)
157
158
12ANEXO DE ORIGINALIDAD
2
3
BIO-NANOTECNOLOGIacuteA APLICADA A LA
REGENERACIOacuteN OacuteSEA MEDIANTE EL
TRANSPORTE DE BIOMOLEacuteCULAS USANDO
NANOPARTIacuteCULAS POLIMEacuteRICAS ESTUDIO
IN VITRO
por
Inmaculada Ortega Oller
Licenciada en Odontologiacutea
Directores de la Tesis
Dr D Joseacute Manuel Peula
Prof Titular de Fiacutesica Aplicada
Dr D Francisco OacuteValle Ravassa
Prof Catedraacutetico de Anatomiacutea Patoloacutegica
Dr D Pablo Galindo Moreno
Prof Catedraacutetico de Estomatologiacutea
4
A mi familia
y directores y en especial
a mi PADRE
Juan Ortega Navarro
5
Agradecimientos
Despueacutes de un apasionado y largo periacuteodo de elaboracioacuten de esta tesis doctoral hoy es el diacutea
escribo este apartado de agradecimientos para finalizar un arduo trabajo Ha sido un periacuteodo de
aprendizaje intenso no solo en el campo cientiacutefico sino tambieacuten a nivel personal que ha supuesto
un gran impacto en miacute Por tal motivo me gustariacutea agradecer a todas aquellas personas que me
han ayudado y brindado su apoyado durante este proceso
En primer lugar quiero agradecer a mis tutores
Don Jose Manuel Peula Garciacutea Profesor Titular de Fiacutesica Aplicada quien con sus
conocimientos y apoyo me guioacute a traveacutes de cada una de las etapas de este proyecto para alcanzar
los resultados que buscaba Gracias por su paciencia calma y tranquilidad para explicar
detenidamente a una odontoacuteloga todos y cada uno de los conceptos aprendidos e interiorizados
Todo mi agradecimiento por su tiempo dedicacioacuten y por haberme acogido de la manera que lo
hizo daacutendome todo su carintildeo apoyo y compresioacuten
Don Pablo Galindo Moreno Profesor Catedraacutetico de Estomatologiacutea
Un trabajo de investigacioacuten es siempre fruto de ideas proyectos y esfuerzos previos que
corresponden a otras personas y que te escogen a ti para saber llevarlas a cabo En este caso mi
maacutes sincero agradecimiento a usted con cuyo trabajo estareacute siempre en deuda y con quien he
compartido proyectos e ilusiones durante todos estos antildeos Gracias por su amabilidad para
facilitarme su tiempo y sus ideas Ha sido una fuente de paz en tiempos muy duros
6
Don Francisco OacuteValle Ravassa Profesor Catedraacutetico de Anatomiacutea Patoloacutegica por su
orientacioacuten atencioacuten a mis consultas y por sus valiosas sugerencias en momentos de duda asiacute
como por su completa disponibilidad siempre que lo he necesitado
Tambien quiero agradecer a Mis Iberica SL y a la Consejeriacutea de Economiacutea Innovacioacuten
Educacioacuten Ciencia y Empleo de la Junta de Andaluciacutea por brindarme todos los recursos y
herramientas que fueron necesarios para llevar a cabo el proceso de investigacioacuten No hubiese
podido arribar a estos resultados sin su incondicional ayuda
Un trabajo de investigacioacuten es tambieacuten fruto del reconocimiento y del apoyo vital que nos
ofrecen las personas que nos estiman sin el cual no tendriacuteamos la fuerza y energiacutea que nos anima
a crecer como personas y como profesionales este es el caso del Dr Don Miguel Padial y de las
Dras Dontildea Azahara Rata-Aguilar Dontildea Ana Jodar y DontildeaTeresa Del Castillo Gracias por
vuestra cercana visioacuten de todo Tambien queriacutea dedicar unas palabras a un buen amigo Javier
Vidao quien con sus conocimientos informaacuteticos me ha ayudado con la elaboracioacuten de este
documento de tesis Gracias
Quiero agradecer tambieacuten a todos mis compantildeeros y amigos por hacer feliz mi dia a dia y a mi
familia por apoyarme auacuten cuando mis aacutenimos decaiacutean A mis hermanos Juan Javier y Dorothy y
a mi madre que siempre estuvieron ahiacute para darme palabras de apoyo y un abrazo reconfortante
para renovar energiacuteas
Gracias a mi pareja por su paciencia comprensioacuten y solidaridad con este proyecto por el
tiempo que me ha concedido un tiempo robado al disfrute conjunto que ha respetado y valorado
Te agradezco la esperanza que me has brindado en los momentos y situaciones mas tormentosas
de mi vida
7
Por uacuteltimo dedico con todo mi corazoacuten mi tesis doctoral a mi PADRE a quien perdimos
recientemente pues sin eacutel no habriacutea logrado nada de esto gracias por haberme forjado como la
persona que hoy soy
Muchos de mis logros se los debo a eacutel quien me educoacute con firmeza pero a la vez sabiendo
darme la libertad suficiente para permitirme evolucionar por mi misma como persona
motivandome constantemente a alcanzar mis metas Fue mi referente en la vida y quien me dioacute el
uacuteltimo y maacutes grande de los aprendizajes dejando en miacute un gran espiacuteritu de lucha sacrificio
esfuerzo y sabiduriacutea ante la maacutes difiacutecil situacioacuten planteable Por todo ello este trabajo te lo dedico
a ti alliacute donde esteacutes espero que seas feliz y puedas disfrutar de los logros conseguidos aquiacute
A todos muchas gracias
8
RESUMEN
REGENERACIOacuteN OacuteSEA A PARTIR DE NANOMICROPARTICULAS DE PLGA
CARGADAS DE BMP-2
El aacutecido poli-laacutectico-co-glicoacutelico (PLGA) es uno de los poliacutemeros sinteacuteticos maacutes ampliamente
utilizados para el desarrollo de sistemas de administracioacuten de faacutermacos y biomoleacuteculas
terapeacuteuticas asi como componente principal en aplicaciones de ingenieriacutea de tejidos Sus
propiedades y versatilidad le permiten ser un poliacutemero de referencia en la fabricacioacuten de
nanopartiacuteculas y micropartiacuteculas para encapsular y liberar una amplia variedad de moleacuteculas
hidrofoacutebicas e hidrofiacutelicas Ademaacutes sus propiedades de biodegradabilidad y biocompatibilidad
hacen del mismo un candidato idoacuteneo para encapsular biomoleacuteculas como proteiacutenas o aacutecidos
nucleicos permitiendo su liberacioacuten de forma controlada
Este trabajo se centra en el uso de nanopartiacuteculas (NP) de PLGA como un sistema de entrega
de uno de los factores de crecimiento maacutes comuacutenmente utilizados en la ingenieriacutea del tejido oacuteseo
la proteiacutena morfogeneacutetica oacutesea 2 (BMP2) Por lo tanto examinamos todos los requisitos
necesarios para alcanzar una correcta encapsulacioacuten y una liberacioacuten controlada y sostenida de
BMP2 utilizando partiacuteculas de PLGA como componente principal discutiendo todos los
problemas y soluciones que hemos encontrado para el desarrollo adecuado de este sistema con un
gran potencial en el proceso de diferenciacioacuten celular y proliferacioacuten bajo el punto de vista de la
regeneracioacuten oacutesea
Hemos desarrollado y optimizando dos meacutetodos de formulacioacuten diferentes para obtener NP de
PLGA cargadas con una proteiacutena modelo con actividad enzimaacutetica como la lisozima que posee
caracteriacutesticas similares a la BMP2 Estas formulaciones se basan en una teacutecnica de doble
emulsioacuten con evaporacioacuten de solvente (agua aceite agua WOW) Se diferencian
principalmente en la fase en la que se agrega el surfactante (Pluronicreg F68) agua (W-F68) o
9
aceite (O-F68) Este surfactante polimeacuterico no ioacutenico puede modular una serie de propiedades del
nanosistema transportador en el que se integra reduciendo el tamantildeo de las NPs incrementando
su estabilidad coloidal y facilitando la proteccioacuten de la biomoleacutecula encapsulada Ademaacutes gracias
a su disposicioacuten superficial y la hidrofilidad de sus colas polares se reduce la interaccioacuten con el
sistema fagociacutetico mononuclear con una mejora de la biodistribucioacuten al aumentar su tiempo de
circulacioacuten despueacutes de una administracioacuten intravenosa en un organismo vivo
Analizamos las propiedades coloidales de estos sistemas usando diferentes teacutecnicas
experimentales (morfologiacutea por SEM y STEM tamantildeo hidrodinaacutemico por DLS y NTA
movilidad electroforeacutetica estabilidad temporal en diferentes medios) asiacute como la encapsulacioacuten
patroacuten de liberacioacuten y bioactividad de la lisozima Asimismo realizamos una caracterizacioacuten
interfacial de la interaccioacuten surfactante-proteiacutena en la primera emulsioacuten agua-aceite para cada
procedimiento de formulacioacuten mediante el anaacutelisis de la tensioacuten superficial y la elasticidad
Finalmente examinamos la captacioacuten celular por ceacutelulas estromales mesenquimaacuteticas humanas y
la citotoxicidad para ambos nanosistemas
Mediante las dos formulaciones O-F68 y W-F68 se obtienen NPs soacutelidas de morfologiacutea
esfeacuterica si bien en un caso el sistema presenta monodispersidad con diaacutemetros alrededor de 120
nm (O-F68) en el otro se obtiene un nanosistema polidisperso con diaacutemetros de partiacutecula
comprendidos entre 100 y 500 nm (W-F68) Como resultado maacutes relevante observamos que la
eficacia de encapsulacioacuten la liberacioacuten y la bioactividad de la lisozima se han mantenido mejor
con el meacutetodo de formulacioacuten W-F68 En este caso dada la heterogeneidad de tamantildeos se podriacutea
hablar de un prometedor sistema multimodal para encapsular proteiacutenas con una fuerte actividad
bioloacutegica que permita una ldquoentrega dualrdquo a nivel extra- e intracelular facilitando la actividad
proteica en la superficie celular y en el citoplasma
Tras desarrollar y optimizar el meacutetodo de siacutentesis para las NPs de PLGA cargadas de lisozima
tratamos de adaptar la formulacioacuten para conseguir la encapsulacioacuten de la proteiacutena terapeacuteutica
BMP-2 Asiacute basaacutendonos en los resultados obtenidos con la lisozima se ha optado por usar el
10
procedimento de siacutentesis W-F68 para favorecer la proteccioacuten de las moleacuteculas proteicas y su
actividad bioloacutegica Con esta formulacioacuten se han obtenido con buena reproducibilidad NPs
esfeacutericas con el tamantildeo multimodal referido anteriormente entre 100 y 500 nm que posibilitaraacuten
el suministro extra- e intracelular Ademaacutes de NPs con BMP2 encapsulada obtenemos un
nanosistema en el que la BMP2 no estaacute encapsulada sino co-adsorbida superficialmente junto a
una proteiacutena estabilizadora como la albuacutemina de suero bovino De nuevo se lleva a cabo una
completa caracterizacioacuten fisico-quiacutemica y bioloacutegica de ambos sistemas de NPs analizando las
propiedades indicadas previamente esto es morfologiacutea y tamantildeo carga superficial estabilidad
coloidal y temporal encapsulacioacuten y patroacuten de liberacioacuten Es conocido que la cineacutetica de
liberacioacuten en los sistemas polimeacutericos basados en PLGA dependen en gran medida de la
degradacioacuten hidroliacutetica del poliacutemero Sin embargo la liberacioacuten a tiempos cortos estaacute influenciada
por otros procesos fiacutesicos y es crucial evitar una descarga inicial excesiva sobre todo si se quiere
optimizar la aplicacioacuten de esta nanotecnologiacutea en procesos de regeneracioacuten oacutesea muy importantes
en odontologiacutea En consecuencia hemos incidido en el anaacutelisis del patroacuten de liberacioacuten de la
BMP2 a tiempos cortos utilizando diferentes teacutecnicas y comparando el comportamiento de los dos
sistemas de NPs con la proteiacutena encapsulada o adsorbida superficialmente
Finalmente se ha analizado la actividad bioloacutegica de las NPs cargadas con BMP2 mediante
estudios in vitro de proliferacioacuten celular migracioacuten y diferenciacioacuten osteogeacutenica usando para ello
ceacutelulas estromales mesenquimales obtenidas a partir de hueso alveolar humano (ABSC) En base
a todo esto se puede confirmar que las NPs con BMP2 encapsuladas presentan un patroacuten de
liberacioacuten adecuado a corto plazo manteniendo un suministro proteico sostenido y una actividad
bioloacutegica adecuada para dosis iniciales de BMP2 muy reducidas
11
SUMMARY
BONE REGENERATION FROM PLGA NANOMICROPARTICLES LOADED
WITH BMP-2
Poly-lactic-co-glycolic acid (PLGA) is one of the most widely used synthetic polymers for the
development of drug delivery systems and therapeutic biomolecules and as a component of tissue
engineering applications Its properties and versatility allow it to be a reference polymer in the
manufacture of nanoparticles and microparticles to encapsulate and release a wide variety of
hydrophobic and hydrophilic molecules Furthermore its biodegradability and biocompatibility
properties make it an ideal candidate for encapsulating biomolecules such as proteins or nucleic
acids that can be released in a controlled manner This work focuses on the use of PLGA
nanoparticles (NP) as a delivery system for one of the most commonly used growth factors in
bone tissue engineering bone morphogenetic protein 2 (BMP2) Therefore we examine all the
necessary requirements to achieve a correct encapsulation and a controlled and sustained release
of BMP2 using PLGA particles as the main component discussing all the problems and solutions
that we have found for the proper development of this system with great potential in the process
of cell differentiation and proliferation from the point of view of bone regeneration We have
developed and optimized two different formulation methods to obtain PLGA NP loaded with a
model protein with enzymatic activity such as lysozyme with similar characteristics to BMP2
These formulations are based on a double emulsion technique with solvent evaporation
(wateroilwater WO W) They differ mainly in the phase in which the surfactant (Pluronicreg
F68) is added water (W-F68) or oil (O-F68) This non-ionic polymeric surfactant can modulate
a series of properties of the transporter nanosystem in which it is integrated reducing the size of
the NPs increasing their colloidal stability and facilitating the protection of the encapsulated
biomolecule Furthermore thanks to its superficial arrangement and the hydrophilicity of its polar
12
tails interaction with the mononuclear phagocytic system is reduced with an improvement in
biodistribution by increasing its circulation time after intravenous administration in a living
organism The colloidal properties of these systems have been analyzed using different
experimental techniques (morphology by SEM and STEM hydrodynamic size by DLS and NTA
electrophoretic mobility temporal stability in different media as well as the encapsulation
release pattern and bioactivity of the lysozyme Likewise an interfacial characterization of the
surfactant-protein interaction was carried out in the first water-oil emulsion for each formulation
procedure by analyzing the surface tension and elasticity Finally we analyzed the cellular uptake
by human mesenchymal stromal cells and cytotoxicity for both nanosystems Through the two
formulations O-F68 and W-F68 solid NPs of spherical morphology are obtained although in
one case the system presents monodispersity with diameters around 120 nm (O-F68) while in
the other a Polydisperse nanosystem with particle diameters between 100 and 500 nm (W-F68)
As a more relevant result we observed that the encapsulation efficiency the release and the
bioactivity of lysozyme have been better maintained with the W-F68 formulation method In this
case given the heterogeneity of sizes one could speak of a promising multimodal system to
encapsulate proteins with strong biological activity that allows a dual delivery at the extra- and
intracellular level facilitating protein activity on the cell surface and in the cytoplasm After
developing and optimizing the synthesis method for lysozyme-loaded PLGA NPs we tried to
adapt the formulation to achieve encapsulation of the therapeutic protein BMP-2 Thus based on
the results obtained with lysozyme it was decided to use the W-F68 synthesis procedure to favor
the protection of protein molecules and their biological activity With this formulation spherical
NPs with the aforementioned multimodal size between 100 and 500 nm have been obtained with
good reproducibility which would allow extra- and intracellular delivery In addition to NPs with
encapsulated BMP2 a nanosystem has been obtained in which BMP2 is not encapsulated but is
superficially co-adsorbed with a stabilizing protein such as bovine serum albumin Again a
complete physico-chemical and biological characterization of both NPs systems is carried out
13
analyzing the previously indicated properties that is morphology and size surface charge
colloidal and temporal stability encapsulation and release pattern
It is known that release kinetics in PLGA-based polymer systems are highly dependent on the
hydrolytic degradation of the polymer However the short-time release is influenced by other
physical processes and it is crucial to avoid an excessive initial discharge especially if the
application of this nanotechnology is to be optimized in very important bone regeneration
processes in dentistry Consequently we have focused on the analysis of the release pattern of
BMP2 at short times using different techniques and comparing the behavior of the two NPs
systems with the encapsulated or superficially adsorbed protein Finally the biological activity of
NPs loaded with BMP2 has been analyzed by in vitro studies of cell proliferation migration and
osteogenic differentiation using mesenchymal stromal cells obtained from human alveolar bone
(ABSC) Based on all this it can be confirmed that NPs with encapsulated BMP2 present an
adequate release pattern in the short term maintaining a sustained protein supply and adequate
biological activity for very low initial doses of BMP2
14
LISTA DE PUBLICACIONES
1 Ortega-Oller I Padial-Molina M Galindo-Moreno P OrsquoValle F Joacutedar-Reyes A B
Peula-Garciacutea J M Bone Regeneration from PLGA Micro-Nanoparticles Biomed Res
Int 2015 vol 2015 1ndash18 doi1011552015415289 IF Q2 Rank 82161 JIFpercentil
494 Nordm de citas 33
2 Ortega-Oller I del Castillo-Santaella T Padial-Molina M Galindo-Moreno P Joacutedar-
Reyes A B Peula-Garciacutea J M Dual delivery nanosystem for biomolecules
Formulation characterization and in vitro release Colloids Surfaces B Biointerfaces
2017 159 586ndash595doi 101016jcolsurfb201708027 IF Q1 Rank1272 JIF
percentil 826 Nordmcitas 5
3 del Castillo-Santaella T Ortega-Oller I Padial-Molina M OrsquoValle F Galindo-
Moreno P Joacutedar-Reyes A B Peula-Garciacutea J M Formulation Colloidal
Characterization and In Vitro Biological Effect of BMP-2 Loaded PLGA Nanoparticles
for Bone Regeneration Pharmaceutics 2019 11(8) 388
doi103390pharmaceutics11080388 IF Q1 Rank26267 JIFpercentil 905 Nordmcitas
3
15
16
Iacutendice
0 GLOSARIO (lista de abreviaturas) helliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphellip 19
1 INTRODUCCIOacuteN 23
11 BMPS ACCIOacuteN Y REGULACIOacuteN 24
111 Uso cliacutenico de la BMP-2 27
12 PARTIacuteCULAS COLOIDALES POLIMEacuteRICAS PARA ENCAPSULAR MOLEacuteCULAS HIDROFIacuteLICAS 30
121Meacutetodos de siacutentesis 33
123 Tamantildeo y morfologiacutea de las partiacuteculas 35
13AGENTES ESTABILIZADORES 39
131 Estabilidad coloidal 39
132 Eficacia de encapsulacioacuten y bioactividad 42
14 PATROacuteN DE LIBERACIOacuteN 44
15 VECTORIZACIOacuteN ENTREGA DIRIGIDA 52
16 INGENIERIacuteA TISULAR SOPORTES 3D O ldquoSCAFFOLDSrdquo 53
2 HIPOacuteTESIS 55
3 OBJETIVOS 56
31 OBJETIVO PRINCIPAL 56
32 OBJETIVOS SECUNDARIOS 56
4 NANOSISTEMA DE ENTREGA DOBLE PARA BIOMOLEacuteCULAS FORMULACIOacuteN CARACTERIZACIOacuteN
Y LIBERACIOacuteN IN VITRO 58
41 ANTECEDENTES 58
42 MATERIALES Y MEacuteTODOS 60
421Formulacioacuten de las nanoparticulas 60
422 Limpieza y almacenamiento 61
423 Caracterizacioacuten de las nanoparticulas 62
424 Estabilidad coloidal y temporal en biologiacutea media 63
425 Actividad bioloacutegica e interacciones 64
43 RESULTADOS Y DISCUSIOacuteN 65
17
431 Formulacioacuten de las nanoparticulas 65
432 Caracterizacioacuten de las Nanopartiacuteculas 69
433 Actividad bioloacutegica e interacciones 84
5 FORMULACIOacuteN CARACTERIZACIOacuteN COLOIDAL Y EFECTO BIOLOacuteGICO IN VITRO DE
NANOPARTIacuteCULAS DE PLGA CARGADAS CON BMP-2 PARA LA REGENERACIOacuteN OacuteSEA 96
51 ANTECEDENTES 96
52 MATERIALES Y MEacuteTODOS 98
521Siacutentesis de nanoparticulas 98
522 Caracterizacioacuten de nanopartiacuteculas morfologiacutea tamantildeo concentracioacuten y movilidad electrocineacutetica
101
523 Estabilidad coloidal y temporal en medios bioloacutegicos 101
524 Interacciones celulares 102
53 RESULTADOS Y DISCUSIOacuteN 105
531Formulacioacuten de nanoparticulas 105
532Caracterizacioacuten de nanopartiacuteculas 109
533Actividad bioloacutegica e interacciones 118
6 CONCLUSIONES 125
7 CONFLICTO DE INTERESES 127
8 RECURSOS ECONOacuteMICOS 127
9 BIBLIOGRAFIacuteA 128
10 ANEXO MATERIAL SUPLEMENTARIO 154
- Enlace a videos
11 ANEXO DE PUBLICACIONES 156
-Artiacuteculo 1 Bone regeneration from PLGA Micro-Nanoparticles
-Artiacuteculo 2 Dual delivery nanosystem for biomolecules Formulation characterization and
in vitro release
18
-Artiacuteculo 3 Formulation Colloidal Characterization and In Vitro Biological Effect of
BMP-2 Loaded PLGA Nanoparticles for Bone Regeneration
12 ANEXO DE ORIGINALIDAD 158
19
LISTA DE ABREVIACIONES
NP Nanopartiacuteculas
MP Micropartiacuteculas
MSC Ceacutelulas mesenquimales
BMP Proteiacutena morfogeneacutetica oacutesea
Rh BMP Proteiacutena morfogeneacutetica oacutesea recombinante
BMP I y II Proteiacutena morfogeneacutetica oacutesea receptora I y II
GF Factor de crecimiento
PDGF Factor de crecimiento derivado de plaquetas
FGF Factor de crecimiento de fibroblastos
IGF Factor de crecimiento de insulina
TGF- Factor de crecimiento transformante
RUNX2 Factor de transcripcioacuten
MPS Sistema fagociacutetico mononuclear
hMSC Ceacutelulas mesenquimales humanas
ABSC Ceacutelulas estromales mesenquimales oacutesea
OSX Osterix
LMP Proteiacutena de mineralizacioacuten de dominio Lim
SEM Microscopia electroacutenica de barrido
STEM Microscopia electroacutenica de transmisioacuten de barrido
PLA Aacutecido poli-lactico
PLGA Aacutecido poli-lactico co-glicolico
LYS Lisozima
LYSF Lisozima final (encapsulada)
20
F68 Pluronic (W-F68= en agua) (O-F68= en aceite)
WOW Doble emulsioacuten de agua en aceite
AP Fase acuosa
OP Fase orgaacutenica
SA Albuacutemina seacuterica
BSA Albuacutemina seacuterica bovina
HSA Albuacutemina seacuterica humana
PVA Alcohol poliviniacutelico
PBS Tampoacuten fosfato salino
PB Tampoacuten fosfato
FBS Suero fetal bovino
PEO Oacutexido de polietileno
DCM Diclorometano
EA Acetato de etilo
FITC Isotiocianato de fluoresceiacutena
DPPC Dipalmitoil-fosfatidilcolina
PGE Polietilenglicol
DC Aacutecido dexosicoacutelico
BCA Aacutecido bicinconiacutenico
DTT Ditiotreitol
SDS Duodecil sulfato de sodio
ALP Fosfatasa alcalina
GAPDH Gliceraldehiacutedo-3-fosfato deshidrogenasa
SDS Gel duodecilsulfato de sodio
PI3K Fosfoinositida 3-quinasa
ALP Fosfatasa alcalina
21
SDS-PAGE Electroforesis en gel poliacrilamida con duodecilsulfato soacutedico
PDI Iacutendice de polidispersidad
DLS Dispersioacuten de luz dinaacutemica
EE Eficacia de encapsulacioacuten
SRB Absorbancia de sulforamida
DL Carga del faacutermaco
FDA Administracioacuten de medicamentos y alimentos
RMN Resonancia magneacutetica nuclear
NTA Anaacutelisis de seguimiento de nanopartiacuteculas
NLS Sentildeales de localizacioacuten nuclear
DMEM Medio Eagle modificado con dulbecco
23
1 INTRODUCCIOacuteN
La regeneracioacuten oacutesea es uno de los principales desafiacuteos a los que nos enfrentamos en la cliacutenica
diariamente Inmediatamente despueacutes de la extraccioacuten de un diente los procesos bioloacutegicos
normales remodelan el hueso alveolar limitando en algunos casos la posibilidad de una futura
colocacioacuten de implante En los uacuteltimos antildeos han sido estudiadas diferentes estrategias para llevar
a cabo la preservacioacuten de ese hueso Otras afecciones como el traumatismo la cirugiacutea de
reseccioacuten tumoral o las deformidades congeacutenitas requieren requisitos teacutecnicos y bioloacutegicos auacuten
mayores para generar la estructura oacutesea necesaria para la rehabilitacioacuten oclusal del paciente Para
superar estas limitaciones anatoacutemicas en teacuterminos de volumen oacuteseo existen diferentes enfoques
para mejorar la osteointegracioacuten del implante o para aumentar la anatomiacutea del hueso donde se
colocaraacute el futuro implante (M Padial-Molina P Galindo-Moreno 2009) (Al-Nawas and
Schiegnitz 2014) El injerto oacuteseo autoacutegeno todaviacutea se considera el ldquogold estaacutendarrdquo debido a sus
propiedades osteogeacutenicas osteoconductivas y osteoinductivas (Katranji Fotek and Wang 2008)
(Misch 1987) Sin embargo tambieacuten presenta varias limitaciones incluida la necesidad de una
segunda cirugiacutea disponibilidad limitada y morbilidad en el aacuterea donante (Myeroff and
Archdeacon 2011) Por lo tanto otros biomateriales como injertos alogeacutenicos e injertos
xenogeacutenicos con osteoconductividad y capacidades osteoinductivas (Avila et al 2010) (Froum
et al 2006) fueron propuestos (Galindo-Moreno et al 2007) (Galindo-Moreno et al 2011) asiacute
como biomateriales aloplaacutesticos (Wheeler 1997) con potencial osteoconductivo Todos estos
materiales aunque aceptables no son adecuados en muchas condiciones y generalmente requieren
una consideracioacuten adicional en el proceso de decisioacuten (Wallace and Froum 2003) Ademaacutes la
cantidad y calidad de hueso que se puede obtener con estos materiales a menudo es limitada
El uso de moleacuteculas bioactivas por siacute solas o en combinacioacuten con los materiales descritos
previamente se ha convertido por lo tanto en un aacuterea de intereacutes principal gracias a su alto
potencial Al usar este tipo de procedimientos es importante considerar 1) el meacutetodo de
administracioacuten y 2) la moleacutecula por siacute misma Las moleacuteculas bioactivas pueden transportarse al
24
aacuterea del defecto como una solucioacuten o un gel incrustados en esponjas adheridos a scaffolds soacutelidos
y maacutes recientemente incluidos en partiacuteculas de diferentes tamantildeos Usando estos meacutetodos se
puede acudir a una gran diversidad de biomoleacuteculas como PDGF (factor de crecimiento
derivado de plaquetas) FGF (factor de crecimiento de fibroblastos) IGF (factor de crecimiento
de insulina) RUNX2 osterix (Osx) proteiacutena de mineralizacioacuten de dominio LIM (LMP) BMP
(proteiacutena morfogeacutenica oacutesea) y maacutes recientemente periostin como candidatos potenciales para los
procedimientos de regeneracioacuten dentro de la cavidad oral incluidos los tejidos oacuteseos y
periodontales (Padial-Molina and Rios 2014) (Padial-Molina Volk and Rios 2014) Estas
moleacuteculas se probaron solas o en combinacioacuten con ceacutelulas madre (Behnia et al 2012) utilizando
varias estrategias in vitro e in vivo (Padial-Molina et al 2012)
11 BMPs Accioacuten y regulacioacuten
En regeneracioacuten oacutesea y en particular los factores de crecimiento morfogeneacuteticos oacuteseos (BMP)
son probablemente el grupo de moleacuteculas maacutes comuacuten Desde 1965 cuando Urist (Urist 1965)
demostroacute que las BMPs oacuteseas extraiacutedas podriacutean inducir la formacioacuten de hueso y cartiacutelago cuando
se implantan en tejido animal un alto nuacutemero de artiacuteculos han probado su aplicacioacuten in vivo y su
base bioloacutegica cuando se usan en defectos oacuteseos (Boyne and Jones 2004) (Wang et al 1990)
(Wozney 1992) Las BMPs son miembros de la suacuteper familia de proteiacutenas TGF-β (Barboza
Caula and Machado 1999) La familia de proteiacutenas BMP agrupa maacutes de 20 proteiacutenas
morfogeneacuteticas homodimeacutericas o heterodimeacutericas que funcionan en muchos tipos y tejidos
celulares no todos tienen que ser necesariamente osteogeacutenicos (Ana Claudia Carreira et al
2014) Las BMPs se pueden dividir en 4 subfamilias seguacuten su funcioacuten y secuencia siendo BMP-
2 -4 y -7 las que tienen un fuerte potencial osteogeacutenico (Ana Claudia Carreira et al 2014) Las
acciones de las BMPs incluyen la condrogeacutenesis la osteogeacutenesis la angiogeacutenesis y la siacutentesis de
la matriz extracelular (Bustos-Valenzuela et al 2011) Dentro de esta familia de proteiacutenas BMP-
2 ha sido la maacutes estudiada Tiene propiedades osteoinductoras que promueven la formacioacuten de
25
nuevo hueso al iniciar estimular y amplificar la cascada de la formacioacuten oacutesea a traveacutes de la
quimiotaxis y la estimulacioacuten de la proliferacioacuten y diferenciacioacuten del linaje celular osteoblaacutestico
(Myeroff and Archdeacon 2011) (Boyne and Jones 2004) (Wozney 1992) (Barboza Caula and
Machado 1999) La ausencia de eacutesta como se estudioacute en los modelos eliminatorios conduce a
fracturas espontaacuteneas que no cicatrizan con el tiempo (Tsuji et al 2006) De hecho otros modelos
han demostrado que la ausencia de cualquiera de estas dos BMP-4 (Tsuji et al 2008) o -7 (Tsuji
et al 2010) no conducen a la formacioacuten de hueso y deterioro como demuestra el efecto producido
por BMP-2 sola (Chen Deng and Li 2012)
Muchos tipos de ceacutelulas en el tejido oacuteseo producen BMP como las ceacutelulas osteoprogenitoras
osteoblastos condrocitos plaquetas y ceacutelulas endoteliales Esta BMP secretada se almacena en la
matriz extracelular donde interactuacutea principalmente con el colaacutegeno tipo IV (Ramel and Hill
2012) Durante los procesos de reparacioacuten y remodelacioacuten la actividad absorbente de los
osteoclastos induce la liberacioacuten de BMP al medio para que se suspenda la funcioacuten de absorcioacuten
y eacutesta pueda interactuar con las ceacutelulas cercanas para iniciar el consecuente proceso osteogeacutenico
(A C Carreira et al 2014)
La BMP en la matriz extracelular se une a los receptores de la superficie celular BMPR-I y II
y activa las proteiacutenas citoplasmaacuteticas Smad o la viacutea MAPK (Deschaseaux Sensebe and Heymann
2009) Cuando BMPR-I se activa BMPR-II se engancha y se activa tambieacuten (Mueller and Nickel
2012) La activacioacuten del complejo BMPR-I y BMPR-II conduce a la activacioacuten de varios Smads
(1 5 y 8) que tambieacuten activan Smad-4 y todos forman complejos proteicos que se transportan al
nuacutecleo donde Runx2 Dlx5 y los genes Osterix (importantes en la osteogeacutenesis) se activan (Chen
Deng and Li 2012) (Ramel and Hill 2012) (Figura 1) De forma similar cuando se activa la ruta
de MAPK conduce a la induccioacuten de la transcripcioacuten de Runx2 y por lo tanto a la diferenciacioacuten
oacutesea (Sieber et al 2009) Tambieacuten se han descrito varios antagonistas extracelulares e
intracelulares que incluyen noggin chordin y gremlin o Smad-6 -7 y -8b respectivamente
(Sapkota et al 2007)
26
Figura 1 Representacioacuten esquemaacutetica de la ruta molecular principal de BMP a la
osteogeacutenesis Las BMP interactuacutean con los receptores de la superficie celular I y II para activar
Smads 1 5 y 8 Estos Smads activados activan Smad 4 Todos juntos como un complejo de
proteiacutenas activan Runx2 Dlx5 y Osterix
Foto tomada de Articulo Ortega-Oller I Padial-Molina M Galindo-Moreno P OacuteValle F
Jodar-Reyes AB Peula-Garcia JM Bone regeneration form Plga Micro-Nanoparticles BioMed
Research International 2015 415289 (2015)
27
111Uso cliacutenico de la BMP-2
Hoy en diacutea la BMP-2 estaacute disponible comercialmente bajo diferentes nombres de marcas y
concentraciones Por lo general consiste en una esponja absorbible de colaacutegeno fijada con BMP-
2 humana recombinante En 2002 fue aprobado por la FDA como una alternativa de injerto oacuteseo
autoacutegeno en la fusioacuten intersomaacutetica lumbar anterior (McKay Peckham and Badura 2007) Maacutes
tarde en 2007 la FDA aproboacute el uso de rhBMP-2 como una alternativa para el injerto oacuteseo
autoacutegeno en el aumento de los defectos de la cresta alveolar asociados con la extraccioacuten del diente
en la neumatizacioacuten del seno maxilar (McKay Peckham and Badura 2007)
Ademaacutes de las aplicaciones en estudios cliacutenicos de columna donde se usan concentraciones
muy altas (AMPLIFYTM rhBMP-2 40 mg) los estudios cliacutenicos han apoyado su uso en la
cavidad oral Las BMP se han utilizado en la regeneracioacuten periodontal la terapeacuteutica oacutesea la
osteointegracioacuten de implantes la cirugiacutea oral con fines ortodoacutencicos la reparacioacuten de secuelas
derivadas de la patologiacutea oacutesea la osteogeacutenesis por distraccioacuten y la cirugiacutea reparadora de
endodoncia (A C Carreira et al 2014) (Hong et al 2013) Sin embargo han mostrado resultados
maacutes prometedores en casos en los que solo se regeneraraacute el tejido oacuteseo incluido el desarrollo del
sitio pre-implantario la elevacioacuten de seno el aumento de cresta vertical y horizontal y la
cicatrizacioacuten de cirugiacuteas de implantes dentales (Spagnoli and Marx 2011) En este sentido se
evidencioacute que el uso de rhBMP-2 indujo la formacioacuten de hueso adecuado para la colocacioacuten de
implantes dentales y su osteointegracioacuten (Nevins et al 1996) Ademaacutes parece que el hueso recieacuten
formado tiene propiedades similares al hueso nativo y por lo tanto es capaz de soportar las
fuerzas oclusales que ejerce la dentadura durante su funcioacuten masticatoria (Boyne et al 2005)
En resumen los estudios nombrados concluyeron que rhBMP-2 induce la formacioacuten de nuevo
hueso con una calidad y cantidad comparable al inducido por la cicatrizacioacuten del propio paciente
e incluso en algunos de los casos se informoacute de haber obtenido una cantidad y calidad de hueso
mayor a la que se hubiese obtenido por la viacutea de cicatrizacioacuten normal del paciente (Lee et al
2013)
28
Por el contrario estudios recientes revelan graves complicaciones despueacutes de su uso (Ronga et
al 2013) Ademaacutes se han asociado efectos carcinogeacutenicos a altas dosis lo que llevoacute a los autores
a enfatizar en la necesidad de mejores pautas en el uso cliacutenico de BMP (Devine et al 2012) No
tan draacutesticos son los uacuteltimos estudios que destacan los efectos secundarios negativos y los riesgos
de su aplicacioacuten haciendo gran hincapieacute en el sesgo potencial de la investigacioacuten patrocinada por
la industria no reproducible especialmente cuando se utiliza en la meacutedula espinal (Fu et al 2013)
(Carragee Hurwitz and Weiner 2011) (Simmonds et al 2013) Se observoacute tambieacuten que el uso
de rhBMP-2 aumenta el riesgo de complicaciones en la zona tratada disfagia con alta eficacia y
dantildea la tergiversacioacuten mediante informes selectivos publicaciones duplicadas y subregistros (Fu
et al 2013) Especiacuteficamente en el campo de la regeneracioacuten oacutesea dentro de la cavidad oral un
estudio de elevacioacuten de seno concluyoacute que el uso de BMP-2 promueve efectos negativos en la
formacioacuten oacutesea cuando se combina con matriz oacutesea bovina inorgaacutenica vs hueso bovino
inorgaacutenico solo (Kao et al 2012) en contraste con artiacuteculos y revisiones previas (Torrecillas-
Martinez et al 2013) Al tomar en cuenta esta informacioacuten se puede concluir que es de extrema
importancia tener cuidado con el uso cliacutenico de nuevos productos evitando las aplicaciones no
clasificadas Tambieacuten es importante resaltar la necesidad de maacutes y mejores investigaciones
cliacutenicas
Para superar estas limitaciones el uso de ceacutelulas mesenquimales especificas (MSC) autoacutelogas
modificadas por BMP-2 ex vivo (Chung et al 2012) en los uacuteltimos antildeos estaacute dando lugar a
explorar nuevas estrategias como la encapsulacioacuten de la proteiacutena en diferentes biomateriales o el
suministro mediante terapia geacutenica
El desarrollo de estas tecnologiacuteas se basa en algunos hechos bioloacutegicos Los efectos in vitro de
las BMP se observan en dosis muy bajas (5-20 ngml) aunque las rhBMP actuales disponibles
comercialmente se usan en dosis grandes (hasta 40 mg de algunos productos) (A C Carreira et al
2014) Esto probablemente se deba a un consumo proteoliacutetico intenso durante las primeras fases
posquiruacutergicas Es importante conocer la secuencia adecuada de los procesos bioloacutegicos que
29
conducen a la cicatrizacioacuten normal del tejido Por lo tanto este conocimiento se puede usar para
intervenir en el marco temporal especiacutefico en el que se pretende que actuacutee nuestra terapia (Padial-
Molina et al 2012) Tambieacuten es importante tener en cuenta que el papel de otras viacuteas moleculares
y la diafoniacutea entre los diferentes componentes que llevan a cabo la regeneracioacuten oacutesea todaviacutea no
se entiende perfectamente y por lo tanto se debe realizar maacutes investigacioacuten
Lo que hasta ahora se sabe en resumen es que las BMP y especiacuteficamente BMP-2 son uacutetiles
para promover la regeneracioacuten oacutesea (A C Carreira et al 2014) Sin embargo las rutas disponibles
de administracioacuten local basadas en la activacioacuten de las BMP entregadas por esponjas de colaacutegeno
presentan importantes limitaciones (Chung et al 2012) En primer lugar la proteiacutena se inactiva
raacutepidamente Por lo tanto su accioacuten bioloacutegica desaparece puede ser incluso antes de que se forme
el coaacutegulo de sangre el cual se forma despueacutes de la cirugiacutea Ademaacutes la distribucioacuten de la BMP
en una suspensioacuten liacutequida incrustada en una esponja de colaacutegeno hace que sea imposible estar
seguro de que la proteiacutena estaacute alcanzando el objetivo ideal Debido a eso deben desarrollarse
nuevas formas de administracioacuten de BMP-2 Estas nuevas tecnologiacuteas tienen que garantizar una
mayor vida media de la proteiacutena y una liberacioacuten escalonada para aumentar los efectos sobre los
objetivos celulares deseados La biotecnologiacutea abre la puerta para poder proporcionar una
solucioacuten a estas limitaciones
De esta manera las nanopartiacuteculas biodegradables (nanoesferas y nanocaacutepsulas) fueron
desarrolladas como una herramienta importante y prometedora para la administracioacuten de
macromoleacuteculas a traveacutes de aplicaciones parenterales mucosas y toacutepicas (Barratt 2003)
(Bramwell and Perrie 2005) (Csaba Garcia-Fuentes and Alonso 2006) (M J Santander-Ortega
et al 2010) Los poliacutemeros biodegradables bien establecidos tales como poli (aacutecido D L-laacutectico)
o poli (D L-laacutectico-co-glicoacutelico) se estaacuten utilizando ampliamente en la preparacioacuten de
nanopartiacuteculas en las uacuteltimas deacutecadas debido a su biocompatibilidad y biodegradabilidad
completa (Jiang et al 2005) Sin embargo se sabe que ciertas macromoleacuteculas como proteiacutenas
o peacuteptidos pueden perder actividad durante su encapsulacioacuten almacenamiento administracioacuten y
30
liberacioacuten (Kumar Soppimath and Nachaegari 2006) Para superar este problema la adicioacuten de
estabilizadores tales como oacutexido de polietileno (PEO) o la co-encapsulacioacuten con otras
macromoleacuteculas y sus derivados parece ser una estrategia prometedora
12 Partiacuteculas coloidales polimeacutericas para encapsular moleacuteculas hidrofiacutelicas
Generalmente las partiacuteculas coloidales polimeacutericas son sistemas consistentes con una forma
esfeacuterica homogeacutenea compuesta por poliacutemeros naturales o sinteacuteticos Con el fin de encapsular
moleacuteculas hidroacutefilas como proteiacutenas o aacutecidos nucleicos para ello es necesario optimizar la
composicioacuten polimeacuterica y el meacutetodo de siacutentesis En este proceso se debe lograr una alta eficacia
de encapsulacioacuten el mantenimiento de la actividad bioloacutegica de la biomoleacutecula encapsulada y la
obtencioacuten de un patroacuten de liberacioacuten adecuado (Danhier et al 2012) (Kumari Yadav and Yadav
2010) (Makadia and Siegel 2011) Varios sistemas de administracioacuten de BMP2 (y otros GF) que
usan partiacuteculas polimeacutericas estaacuten descritos en la bibliografiacutea La mayoriacutea de ellos son sistemas
microparticulados Casi en su totalidad todos ellos usan el copoliacutemero de PLGA biocompatible
y biodegradable como componente principal (Mohamed and van der Walle 2008) (Silva et al
2007)
Teniendo en cuenta la incorporacioacuten de BMP2 al sistema portador la encapsulacioacuten es
preferible a la adsorcioacuten porque los factores de crecimiento estaacuten maacutes protegidos contra factores
ambientales en el medio y pueden tener un mejor control sobre la administracioacuten y liberacioacuten para
alcanzar las concentraciones deseadas en sitio y tiempo especiacuteficos (Zhang and Uludag 2009)
Normalmente si los GF estaacuten relacionados con los procesos de regeneracioacuten oacutesea las nano-
micropartiacuteculas quedan atrapadas en un segundo sistema como hidrogeles o scaffolds de
ingenieriacutea tisular que tambieacuten juegan un papel importante en el perfil de liberacioacuten de los GF de
estas partiacuteculas (Zhang and Uludag 2009) Las nano-micropartiacuteculas han permitido el desarrollo
de scaffolds multiescala lo que facilita el control de la arquitectura interna y los patrones
31
adecuados de los gradientes mecaacutenicos de las ceacutelulas asiacute como los factores de sentildealizacioacuten (Santo
et al 2012)
Todos los pasos desde el meacutetodo de siacutentesis y sus caracteriacutesticas el proceso de encapsulacioacuten
o la modificacioacuten final de la superficie para una entrega dirigida determinan las caracteriacutesticas de
estos sistemas y su objetivo principal la liberacioacuten controlada de GF bioactivos
Figura 2 Procedimiento de doble emulsioacuten (emulsioacuten agua aceite agua W1OW2) para
obtener micropartiacuteculas nanopartiacuteculas de PLGA
En la figura 2 se muestra el esquema de siacutentesis de micro-nanopartiacuteculas de PLGA mediante
un procedimiento de doble emulsioacuten Dependiendo de las condiciones de siacutentesis (estabilizadores
disolventes y procedimiento de mezcla) es posible obtener micro-nanoesferas con una matriz
uniforme o micro-nanocaacutepsulas con una estructura corteza-nuacutecleo Las inmunopartiacuteculas
32
utilizadas para la administracioacuten dirigida pueden obtenerse uniendo moleacuteculas especiacuteficas de
anticuerpos en la superficie de la partiacutecula
33
121Meacutetodos de siacutentesis
Es posible encontrar varios procedimientos para encapsular moleacuteculas hidrofiacutelicas como
proteiacutenas o aacutecidos nucleicos en nano micropartiacuteculas polimeacutericas Se ha observado que las
teacutecnicas de separacioacuten de fases (Tran Swed and Boury 2012) o secado por pulverizacioacuten (Ertl et
al 2000) encapsulan moleacuteculas hidroacutefilas Sin embargo en el caso de las proteiacutenas el
procedimiento maacutes utilizado para encapsularlas en micropartiacuteculas y nanopartiacuteculas de PLGA es
la teacutecnica de evaporacioacuten con disolvente de doble emulsioacuten (WOW) (Makadia and Siegel 2011)
(Hans and Lowman 2002) En la figura 2 se presenta una descripcioacuten esquemaacutetica de esta teacutecnica
De manera general el PLGA se disuelve en un disolvente orgaacutenico y se emulsiona usando
agitacioacuten mecaacutenica o sonicacioacuten con agua que contiene una cantidad apropiada de proteiacutena
Por lo tanto se obtiene una emulsioacuten W1O primaria En la segunda fase esta emulsioacuten se
vierte en una gran fase polar que conduce a una precipitacioacuten inmediata de las partiacuteculas como
consecuencia de la contraccioacuten del poliacutemero alrededor de las gotitas de la emulsioacuten primaria Esta
fase puede estar compuesta por una solucioacuten acuosa de un estabilizador (surfactante) o mezclas
de etanol y agua (Blanco and Alonso 1998) (Csaba et al 2005) Despueacutes de agitar el resultado
del disolvente orgaacutenico se extrae raacutepidamente por evaporacioacuten al vaciacuteo En este procedimiento se
ha probado una amplia lista de diferentes modificaciones con el fin de obtener un sistema micro
nanoportador con estabilidad coloidal adecuada alta eficacia de encapsulacioacuten bioactividad
adecuada y finalmente un perfil de liberacioacuten a largo plazo con una miacutenima descarga inicial
El objetivo es evitar que se libere una gran cantidad de proteiacutena (gt 60) muy raacutepidamente (24
horas) que es uno de los mayores problemas de un sistema de liberacioacuten controlada (Mohamed
and van der Walle 2008)
34
122 Disolvente orgaacutenico
Hans y colaboradores muestran diferentes ejemplos de solventes orgaacutenicos usados en muacuteltiples
procesos de emulsioacuten Normalmente pueden usarse diclorometano (DMC) acetato de etilo
acetona y otras mezclas (Hans and Lowman 2002) En el primer paso seriacutea una buena eleccioacuten
escoger un buen solvente orgaacutenico con baja solubilidad en agua para facilitar el proceso de
emulsioacuten y bajo punto de ebullicioacuten para una faacutecil evaporacioacuten Sin embargo la estructura de las
moleacuteculas de proteiacutenas encapsuladas puede verse afectada y los procesos de desnaturalizacioacuten y
peacuterdida de actividad bioloacutegica aparecen cuando interactuacutean con un solvente orgaacutenico tiacutepico como
DMC (Danhier et al 2012) El acetato de etilo por otro lado ejerce efectos menos
desnaturalizantes con una menor incidencia en la bioactividad de las proteiacutenas encapsuladas
(Sturesson and Carlfors 2000)
Otros factores importantes relacionados con el disolvente orgaacutenico son sus propiedades fiacutesicas
que afectan la forma en que las moleacuteculas del poliacutemero se auto organizan en la envoltura de las
gotas de la emulsioacuten y modifican la morfologiacutea de las nanopartiacuteculas y la eficacia de
encapsulacioacuten (Rosca Watari and Uo 2004) De esta forma una mayor solubilidad en agua del
disolvente orgaacutenico es decir acetato de etilo favorece una eliminacioacuten maacutes raacutepida del disolvente
Ademaacutes la velocidad de eliminacioacuten del disolvente puede controlarse ajustando el volumen de la
fase polar asiacute como la tensioacuten de cizallamiento durante la segunda etapa de la emulsioacuten Un
aumento de estos dos paraacutemetros aumenta la velocidad de difusioacuten del acetato de etilo desde las
micropartiacuteculas primarias a la fase acuosa externa lo que da como resultado su raacutepida
solidificacioacuten (Meng et al 2003) Tambieacuten mejora la eficiencia de encapsulacioacuten y minimiza el
tiempo de contacto entre las moleacuteculas de proteiacutena y el solvente orgaacutenico (Ghaderi and Carlfors
1997) obteniendo al mismo tiempo un menor efecto de raacutefaga y una liberacioacuten maacutes lenta del
faacutermaco desde las micropartiacuteculas (Meng et al 2003)
35
123 Tamantildeo y morfologiacutea de las partiacuteculas
El tamantildeo de la partiacutecula es un paraacutemetro importante y uno de los objetivos principales del
sistema de liberacioacuten polimeacuterica Las microesferas desde unos pocos microacutemetros hasta 100 μm
son adecuadas para el suministro oral la adhesioacuten a la mucosa o el uso interior del armazoacuten es
decir para la regeneracioacuten oacutesea La dimensioacuten a nano-escala del soporte ofrece una versatilidad
mejorada cuando se compara con partiacuteculas de mayor tamantildeo Esto se debe a que tienen una
mayor estabilidad coloidal una mejor dispersabilidad y biodisponibilidad una superficie maacutes
reactiva y ademaacutes pueden administrar proteiacutenas o faacutermacos dentro y fuera de las ceacutelulas
correspondientes (Wang et al 2012) BMP2 promueve la formacioacuten de hueso e induce la
expresioacuten de otras BMP e inicia la viacutea de sentildealizacioacuten desde la superficie de la ceacutelula unieacutendose
a dos receptores de superficie diferentes (Bustos-Valenzuela et al 2011) Por lo tanto las
partiacuteculas portadoras de BMP2 deben liberarlo en el medio extracelular Dado que la ingesta
celular de nanopartiacuteculas de PLGA es muy raacutepida el proceso de incorporacioacuten puede verse
limitado por un aumento en el tamantildeo de nano a micropartiacuteculas (Xiong et al 2011) Sin
embargo la interaccioacuten entre partiacuteculas y ceacutelulas estaacute fuertemente influenciada por el tamantildeo de
la partiacutecula Si se desea la internalizacioacuten de la ceacutelula la partiacutecula debe estar comprendida en la
escala submicroacutemica en un intervalo entre 2-500 nm (Chou Ming and Chan 2011) Ademaacutes este
tamantildeo es necesario para una distribucioacuten raacutepida despueacutes de la administracioacuten parenteral con el
fin de alcanzar diferentes tejidos a traveacutes de diferentes barreras bioloacutegicas Ademaacutes la ingesta de
macroacutefagos se minimiza con un diaacutemetro de nanopartiacuteculas por debajo de 200 nm e incluso maacutes
pequentildeo (Hans and Lowman 2002) (Manuel J Santander-Ortega Lozano-Loacutepez et al 2010)
Como se discutioacute en un artiacuteculo escrito por Yang y colaboradores (Yang Chung and Ng 2001)
ligeras modificaciones del procedimiento de siacutentesis pueden suponer efectos draacutesticos en el
tamantildeo o la morfologiacutea de las partiacuteculas y por lo tanto en la eficacia de encapsulacioacuten de
proteiacutenas y la liberacioacuten cineacutetica
36
Figura 3 Fotografiacutea mediante microscopiacutea electroacutenica de barrido (SEM) de nanopartiacuteculas
de PLGA obtenidas mediante un procedimiento de emulsificacioacuten de doble emulsioacuten Es un
sistema con forma esfeacuterica baja polidispersidad y una escala nanoscoacutepica que muestra las
propiedades deseadas para una distribucioacuten fisioloacutegica adecuada y la internalizacioacuten celular
En los procesos de doble emulsioacuten la primera etapa de emulsioacuten determina en gran medida el
tamantildeo de la partiacutecula mientras que la segunda etapa de emulsioacuten caracterizada por la
eliminacioacuten del disolvente y la precipitacioacuten del poliacutemero afecta principalmente a la morfologiacutea
de la partiacutecula (Rosca Watari and Uo 2004) Sin embargo en este paso el uso de soluciones
surfactantes como el medio polar del segundo proceso de emulsioacuten y la relacioacuten de volumen entre
las fases orgaacutenicas y polares han mostrado una influencia importante en el tamantildeo final (Feczkoacute
Toacuteth and Gyenis 2008)Por lo tanto la eleccioacuten correcta del solvente orgaacutenico la concentracioacuten
del poliacutemero la adicioacuten de surfactante y la energiacutea del proceso de emulsioacuten permiten controlar el
tamantildeo del sistema
37
La incorporacioacuten de poloxaacutemeros (F68) en el disolvente orgaacutenico de la emulsioacuten primaria
ayuda a aumentar la estabilidad coloidal de la primera dispersioacuten al colocarla en el interfaz WO
Esto reduce el tamantildeo de partiacutecula en comparacioacuten con las nanopartiacuteculas de PLGA puro en las
que la uacutenica fuente de estabilidad proviene de la carga eleacutectrica de los grupos carboxilo del PLGA
(Santander-Ortega Bastos-Gonzalez and Ortega-Vinuesa 2007) Es normal obtener micro
nanoesferas ciliacutendricas con un nuacutecleo poroso polimeacuterico En la figura 3 se muestra una
micrografiacutea SEM tiacutepica de nanopartiacuteculas de PLGA obtenidas mediante emulsioacuten WOW usando
una mezcla de disolventes orgaacutenicos (DCM acetona) y etanol agua como segundo medio polar
en la que la forma esfeacuterica y la distribucioacuten uniforme del tamantildeo son las principales
caracteriacutesticas La cubierta exterior polimeacuterica en la segunda etapa de emulsioacuten empujoacute las gotas
de agua hacia el nuacutecleo interno de acuerdo con el proceso de solidificacioacuten (Yang Chia and
Chung 2000) Este proceso permite producir partiacutecula como son estas caacutepsulas con una
estructura nuacutecleo-capa en la que el nuacutecleo interno tiene una baja densidad de poliacutemero
La figura 4 muestra una estructura nuacutecleo-capa tiacutepica en la que el poliacutemero precipita y se
contrae alrededor de las gotas de agua durante el cambio de disolvente de la segunda fase y el
posterior proceso de evaporacioacuten del disolvente orgaacutenico (Fang et al 2014) En este caso el
proceso de solidificacioacuten del poliacutemero se ve influenciado y determinado por la miscibilidad del
disolvente orgaacutenico con la segunda fase polar y la velocidad de eliminacioacuten
38
Figura 4 Nanopartiacuteculas de mezcla PLGA poloxamers188 (a) Fotografiacutea de microscopiacutea
electroacutenica de transmisioacuten (STEM) (b) Fotografiacutea de microscopiacutea electroacutenica de barrido (SEM)
La teacutecnica STEM permite el anaacutelisis de la estructura de nanopartiacuteculas con una regioacuten interna
con baja densidad de poliacutemero que es representativa de nanocaacutepsulas con estructura nuacutecleo-
caparazoacuten
La cubierta polimeacuterica a menudo presenta canales o poros como consecuencia de la extrusioacuten
de agua interna debido a las fuerzas osmoacuteticas Esto puede reducir la eficacia de la encapsulacioacuten
y favorecer una raacutepida fuga inicial con la liberacioacuten en raacutefaga no deseada (Yang Chung and
Ng 2001) Esta modificacioacuten de la estructura interna de las partiacuteculas generalmente se indica
asignando el teacutermino nanoesfera al sistema con un nuacutecleo que consiste en una matriz polimeacuterica
homogeacutenea El agente bioactivo se dispersa dentro de ellas mientras que la estructura nuacutecleo-
capa seriacutea similar a una nanocaacutepsula donde la biomoleacutecula estaacute preferiblemente en la cavidad
acuosa rodeada por la cubierta polimeacuterica (Zhang and Uludag 2009) (ver figura 2)
39
13Agentes estabilizadores
131 Estabilidad coloidal
El meacutetodo de doble emulsioacuten normalmente requiere la presencia de estabilizadores para
conferir estabilidad coloidal durante la primera etapa de emulsioacuten para evitar la coalescencia de
las gotas de la emulsioacuten y maacutes tarde para mantener la estabilidad de las nano micropartiacuteculas
finales (Ratzinger et al 2010) El alcohol poliviniacutelico (PVA) y el derivado de PEO como
poloxaacutemeros se han usado en la mayoriacutea de los casos (Blanco and Alonso 1998) (Feczkoacute Toacuteth
and Gyenis 2008) Otros incluyen surfactantes naturales como los fosfoliacutepidos (Feng and Huang
2001) (Chan et al 2009) En algunos casos es posible evitar los surfactantes si las partiacuteculas
tienen una contribucioacuten de estabilidad electrostaacutetica es decir de los grupos carboxilo terminales
no protegidos de las moleacuteculas de PLGA (Fraylich et al 2008)
Como se ha comentado anteriormente el PVA y los poloxaacutemeros han demostrado su eficacia
en la siacutentesis de nanopartiacuteculas y micropartiacuteculas afectando no solo la estabilidad de los sistemas
sino tambieacuten su tamantildeo y morfologiacutea Por lo tanto se ha encontrado un efecto de reduccioacuten de
tamantildeo usando PVA en la fase acuosa externa que afecta al mismo tiempo la porosidad
superficial principalmente en partiacuteculas de tamantildeo micro (Feczkoacute Toacuteth and Gyenis 2008) Un
estudio comparativo entre PVA y fosfoliacutepidos (di-palmitoil fosfatidilcolina) como estabilizadores
mostroacute que DPPC podriacutea ser un mejor emulsionante que PVA para producir nano y
micropartiacuteculas Con este meacutetodo se necesitaba una cantidad de estabilizador mucho maacutes baja
para obtener un tamantildeo similar En el mismo estudio se demostroacute una mayor porosidad en la
superficie de la partiacutecula para las nanoesferas emulsionadas con PVA (Feng and Huang 2001)
Por otro lado la combinacioacuten de PLGA con poloxaacutemeros ha mostrado efectos positivos para
los nano y microsistemas en teacuterminos de estabilidad eficacia de encapsulacioacuten o caracteriacutesticas
de liberacioacuten controlada (Santander-Ortega et al 2011) El uso de estos surfactantes en el primer
o segundo paso del procedimiento de emulsioacuten WOW conduce a situaciones diferentes Por lo
40
tanto si los poloxaacutemeros se mezclan con PLGA en la fase orgaacutenica de la emulsioacuten primaria se
obtiene una alteracioacuten de la rugosidad superficial Sin embargo si se agregan en la fase de agua
interna se encuentra un aumento de la porosidad (Blanco and Alonso 1998) La inclusioacuten de
poloxaacutemeros en la fase polar de la segunda etapa de emulsioacuten tambieacuten genera superficies de
rugosidad hidroacutefila Una cuantificacioacuten de esto se muestra en la figura 5 en la que se mide la
movilidad electroforeacutetica de PLGA puro y PLGA pluronic F68 nanopartiacuteculas como una funcioacuten
del pH del medio La composicioacuten de superficie diferente afecta el comportamiento
electrocineacutetico de las nanopartiacuteculas desnudas La carga superficial se modula por la presencia de
tensioactivo no ioacutenico como poloxaacutemeros o en mayor medida por la presencia de moleacuteculas de
anticuerpos unidos en la superficie La dependencia observada con este paraacutemetro es una
consecuencia del caraacutecter aacutecido deacutebil de los grupos carboxilo de PLGA Cuando las moleacuteculas de
poloxaacutemero estaacuten presentes en la interfaz se encuentra una reduccioacuten sistemaacutetica de la movilidad
como consecuencia del aumento de la rugosidad superficial Las cadenas de surfactante hidroacutefilo
se dispersan hacia el disolvente originando un desplazamiento del plano de corte y la consecuente
reduccioacuten de movilidad (Santander-Ortega Bastos-Gonzalez and Ortega-Vinuesa 2007)
(Fraylich et al 2008) La presencia de moleacuteculas proteicas en la superficie introduce una
dependencia de la carga neta superficial con el punto isoeleacutectrico de eacutestas situacioacuten que se refleja
en el comportamiento electrocineacutetico que incluso muestras valores positivos en pHs inferiores al
punto isoeleacutectrico del anticuerpo
El tamantildeo final de las partiacuteculas de PLGA se controla principalmente por fuerzas
electrostaacuteticas y no se ve significativamente afectado por la presencia o la naturaleza de los
estabilizadores de poloxaacutemero (Fraylich et al 2008) El reconocimiento de los nanovehiacuteculos por
el sistema fagociacutetico mononuclear (MPS) se puede alterar significativamente si la superficie de
las partiacuteculas coloidales se modifica mediante el uso de copoliacutemeros de bloque PEO de las
moleacuteculas de poloxaacutemero La barrera esteacuterica proporcionada por estas moleacuteculas surfactantes
previenen o minimizan la adsorcioacuten de proteiacutenas plasmaacuteticas y disminuye el reconocimiento por
41
los macroacutefagos (Tan et al 1993) El tamantildeo de las microesferas tampoco se ve afectado por la
encapsulacioacuten de poloxaacutemeros El sistema que contiene mezclas de poloxaacutemero-PLGA conduce
a una estructura interna que muestra pequentildeos orificios y cavidades en relacioacuten con microesferas
de PLGA puro con una estructura de tipo matriz compacta (Blanco and Alonso 1998)
Figura 5 Movilidad electroforeacutetica versus pH para nanopartiacuteculas de PLGA con diferentes
caracteriacutesticas () PLGA (◼) mezcla de PLGA poloxamer188 y () PLGA cubierto por
Immuno-γ-globulina
Las micropartiacuteculas formuladas por poloxaacutemero en el segundo medio polar tienen una
superficie completamente diferente que las de PVA casi sin poros (Feczkoacute Toacuteth and Gyenis
2008) Una comparacioacuten entre diferentes poloxaacutemeros muestra que el balance hidroacutefilo-lipoacutefilo
(HBL) del surfactante juega un papel crucial determinando las interacciones surfactante-poliacutemero
y controlando la porosidad y la rugosidad de las nano-micropartiacuteculas (Blanco and Alonso 1998)
(Bouissou et al 2004)
42
De manera similar a los surfactantes las caracteriacutesticas del poliacutemero como el grado de
hidrofobicidad el peso molecular o la velocidad de degradacioacuten de la hidroacutelisis pueden influir
fuertemente en la morfologiacutea de la partiacutecula Por lo tanto la composicioacuten polimeacuterica de las
partiacuteculas afecta en gran medida su estructura y propiedades Es por eso que es habitual usar otros
poliacutemeros para modificar el comportamiento y la aplicacioacuten de las partiacuteculas De esta manera el
polietilenglicol (PEG) de diferente longitud de cadena se usa frecuentemente para modificar las
caracteriacutesticas de la superficie Con PEG las partiacuteculas son maacutes hidroacutefilas y tienen superficies
maacutes rugosas que afectan la accioacuten de MPS al aumentar el tiempo de circulacioacuten y la vida media
in vivo como la presencia de cadenas de PEO (Gref et al 1994) Ademaacutes las cadenas de PEG
tambieacuten proporcionan estabilidad coloidal a traveacutes de la estabilizacioacuten esteacuterica Las
nanopartiacuteculas o micropartiacuteculas de PLGA se pueden obtener normalmente mediante el uso en el
meacutetodo de siacutentesis de copoliacutemeros de di y tri-bloque de PLGA PEG (Lochmann et al 2010)
(White et al 2013) (Makadia and Siegel 2011) Los poliacutemeros naturales como el quitosano
ademaacutes de aumentar la hidrofobicidad de la superficie tambieacuten les confieren un caraacutecter
mucoadherente (Paolicelli et al 2010)
132 Eficacia de encapsulacioacuten y bioactividad
Ademaacutes el uso de estabilizantes (surfactantes o poliacutemeros) tambieacuten influye en la eficacia de
encapsulacioacuten y la estabilidad de la proteiacutena De hecho para el proceso de evaporacioacuten del
solvente WOW el solvente orgaacutenico clorado usado para la primera emulsioacuten puede degradar las
moleacuteculas de proteiacutena encapsuladas en este paso si entran en contacto con la interfaz orgaacutenica
agua causando su agregacioacuten o desnaturalizacioacuten (Brigger Dubernet and Couvreur 2002) La
interaccioacuten poliacutemero-proteiacutena el estreacutes de cizallamiento para el proceso de emulsioacuten y la
reduccioacuten del pH derivada de la degradacioacuten del poliacutemero PLGA tambieacuten pueden producir la
misma situacioacuten con la peacuterdida posterior de la actividad bioloacutegica de las biomoleacuteculas
encapsuladas Se han usado diferentes estrategias para prevenirlo Por ejemplo un aumento de la
viscosidad alrededor de las moleacuteculas de proteiacutenas puede ayudar a aislarlas de su microambiente
43
(Giteau et al 2008) De esta forma los productos viscosos como el almidoacuten se han utilizado
para prevenir la inestabilidad proteica (Balmayor et al 2009) Estos autores coencapsulan BMP2
con albuacutemina dentro de micropartiacuteculas de almidoacuten usando otro poliacutemero biodegradable poli-ε-
caprolactona en lugar de PLGA La BMP2 retuvo la bioactividad A pesar de una baja tasa de
encapsulacioacuten ademaacutes de una raacutefaga inicial seguida de una liberacioacuten incompleta la cantidad de
BMP2 necesaria al principio fue menor (Balmayor et al 2009) La combinacioacuten de surfactantes
PEO con PLGA (mezclados en la fase orgaacutenica) tambieacuten puede preservar la bioactividad de las
proteiacutenas microencapsuladas (Santander-Ortega et al 2009) o los aacutecidos nucleicos (Csaba et al
2005)
Sin embargo en la mayoriacutea de los casos la estrategia preferida fue la coencapsulacioacuten de
estabilizadores con biomoleacuteculas De este modo las albuacuteminas seacutericas (SA) han demostrado la
capacidad de limitar la agregacioacuten-desestabilizacioacuten de varias proteiacutenas incitadas por el interfaz
agua disolvente orgaacutenico del proceso de emulsioacuten primaria (Meinel et al 2001) (Srinivasan et
al 2005) White y colaboradores en uno de sus estudios encapsularon lisozima dentro de
micropartiacuteculas de PLGA-PEG Ademaacutes de la funcioacuten de proteccioacuten tambieacuten observaron un
aumento importante de la eficiencia de encapsulacioacuten cuando el SA humana se co-encapsuloacute con
lisozima y BMP2 (White et al 2013) Dr Angelo y colaboradores usaron heparina como
estabilizador porque eacutesta forma un complejo especiacutefico con varios factores de crecimiento
estabiliza su estructura tridimensional y promueve su bioactividad Se consiguioacute aumentar asiacute la
eficacia de encapsulacioacuten del 35 al 87 usando SA bovina como un segundo estabilizador para
encapsular dos factores de crecimiento proangiogeacutenico naturales dentro de las nanopartiacuteculas
mezcladas con PLGA-poloxaacutemero Los ensayos celulares in vitro mostraron la preservacioacuten de la
actividad bioloacutegica de GF hasta un mes despueacutes (drsquoAngelo et al 2010)
El uso de maacutes surfactantes hidroacutefilos (poloxaacutemeros) o poliacutemeros (PEG) en la fase acuosa
interna o durante la mezcla del PLGA en la fase orgaacutenica de la emulsioacuten primaria reduce la
interaccioacuten de las proteiacutenas encapsuladas con la matriz de PLGA hidroacutefoba Esto evita la
44
alteracioacuten de la estructura de las moleacuteculas de proteiacutena y ayuda al mismo tiempo a neutralizar la
acidez generada por la degradacioacuten hidroliacutetica del PLGA (Tan et al 1993) En algunos casos se
ha demostrado que la combinacioacuten de varios estabilizadores como poloxaacutemeros tranexaacutemico y
bicarbonato de sodio preserva la integridad de las proteiacutenas encapsuladas pero tambieacuten reduce
la eficacia de la encapsulacioacuten (Bouissou et al 2004)
Como regla general la eficacia de la encapsulacioacuten aumenta con el tamantildeo de las partiacuteculas
(Hans and Lowman 2002) Ademaacutes la estabilizacioacuten adecuada de la emulsioacuten primaria por
poliacutemeros anfifiacutelicos y una solidificacioacuten (precipitacioacuten) raacutepida del poliacutemero en el segundo paso
son paraacutemetros favorables para mejorar la eficacia de encapsulacioacuten de proteiacutenas en la teacutecnica de
emulsioacuten WOW (Meng et al 2003)
La tendencia de BMP2 a interactuar con superficies hidrofoacutebicas puede disminuir la peacuterdida
de proteiacutena encapsulada durante la liberacioacuten de la fase de disolvente Esto favorece una mayor
encapsulacioacuten pero disminuye la liberacioacuten posterior (Lochmann et al 2010) Se obtiene una
encapsulacioacuten proteica oacuteptima cuando el pH de las fases de agua internas y externas estaacuten cerca
del punto isoeleacutectrico de la proteiacutena (Manuel J Santander-Ortega Lozano-Loacutepez et al 2010)
Blanco y Alonso (Blanco and Alonso 1998) observaron una reduccioacuten en la eficacia de
encapsulacioacuten de proteiacutena cuando el poloxaacutemero se coencapsuloacute en la emulsioacuten primaria Esto
pone de relieve el papel principal desempentildeado por la interaccioacuten proteiacutena-poliacutemero en la eficacia
de encapsulacioacuten y el proceso de liberacioacuten posterior Sin embargo demasiado emulsionante
tambieacuten puede dar como resultado una reduccioacuten de la eficacia de encapsulacioacuten (Feng and
Huang 2001) Por lo tanto se necesita un equilibrio entre el polvo de emulsioacuten del surfactante y
su concentracioacuten
14 Patroacuten de liberacioacuten
El patroacuten de liberacioacuten representa una de las caracteriacutesticas maacutes importantes de un sistema
portador de partiacuteculas nano micro ya que su desarrollo tiene un objetivo final principal la
45
liberacioacuten adecuada de las moleacuteculas bioactivas encapsuladas para alcanzar la accioacuten cliacutenica
deseada
Se han definido diferentes patrones de liberacioacuten de proteiacutenas encapsuladas en micropartiacuteculas
nanopartiacuteculas de PLGA Es posible encontrar una liberacioacuten continua cuando la difusioacuten de la
biomoleacutecula es maacutes raacutepida que la erosioacuten de la partiacutecula Este proceso implica una difusioacuten
continua de la proteiacutena que se encuentra en la matriz del poliacutemero antes de que la partiacutecula de
PLGA se degrade en monoacutemeros de aacutecido laacutectico y glicoacutelico por hidroacutelisis (Kumari Yadav and
Yadav 2010) Tambieacuten se ha descrito una liberacioacuten bifaacutesica caracterizada por una descarga
inicial dentro o cerca de la superficie de la partiacutecula seguido por una segunda fase en la que la
proteiacutena se libera progresivamente por difusioacuten La segunda fase se puede mejorar mediante la
erosioacuten masiva del caparazoacuten y la matriz de PLGA lo que da como resultado un importante
aumento de poros y canales (Makadia and Siegel 2011) Se ha encontrado un tercer perfil de
liberacioacuten trifaacutesica cuando se produce un periacuteodo de liberacioacuten de retardo despueacutes de la descarga
inicial y hasta la degradacioacuten del poliacutemero (Cleland 1997) Finalmente es posible obtener una
liberacioacuten de proteiacutena incompleta como consecuencia de factores adicionales relacionados con la
interaccioacuten proteiacutena-poliacutemero o la inestabilidad proteica La Figura 6 ilustra los diferentes perfiles
de liberacioacuten descritos anteriormente Un sistema de transporte oacuteptimo deberiacutea ser capaz de liberar
un gradiente de concentracioacuten controlado de factores de crecimiento en el momento apropiado
evitando o al menos reduciendo o controlando el efecto de descarga inicial (Oh Kim and Lee
2011) Una explosioacuten inicial controlada seguida de una liberacioacuten sostenida mejora
significativamente la regeneracioacuten oacutesea in vivo (Brown et al 2009) (Brown et al 2011) (Li et
al 2009)
Giteau y colaboradores (Giteau et al 2008) presentan una revisioacuten interesante sobre Coacutemo
lograr una liberacioacuten sostenida y completa de micropartiacuteculas de PLGA Comienzan por analizar
la influencia del medio de liberacioacuten y el meacutetodo de muestreo en el perfil de liberacioacuten y resaltan
la importancia del proceso de limpieza por centrifugacioacuten o el volumen del medio de liberacioacuten
46
Ajustando a valores adecuados la velocidad de centrifugacioacuten o el volumen del tampoacuten es posible
separar micro nanopartiacuteculas del medio de liberacioacuten que contiene proteiacutenas de una manera muy
faacutecil Esto permite patrones de liberacioacuten estables y reproducibles Por otro lado para garantizar
un mejor perfil de liberacioacuten de proteiacutenas se debe realizar la modificacioacuten de la formulacioacuten de
micropartiacuteculas y el proceso de microencapsulacioacuten para preservar la agregacioacuten de proteiacutenas La
estabilidad de la proteiacutena debe mantenerse evitando la formacioacuten de un medio dantildeino Por
ejemplo la formulacioacuten de una siacutentesis en concreto puede modificarse para usar poliacutemeros maacutes
hidroacutefilos ya que se ha demostrado que reducen el estallido inicial y proporcionan proteiacutenas
bioactivas durante largos periodos de tiempo
47
Figura 6 Patroacuten de liberacioacuten (liacutenea negra) Cineacutetica de liberacioacuten de BSA en nanopartiacuteculas
de PLGA con alta liberacioacuten inicial (liacutenea de puntos rojos) modelo bifaacutesico que combina un
estallido inicial moderado y una liberacioacuten sostenida posterior (liacutenea de trazos azules) modelo
trifaacutesico con un retraso de liberacioacuten entre las fases de liberacioacuten inicial y sostenida (liacutenea verde
de guiones) liberacioacuten incompleta
Las estrategias maacutes relevantes se mencionan a continuacioacuten La liberacioacuten de faacutermaco a partir
de nano micropartiacuteculas de PLGA puede controlarse por el peso molecular del poliacutemero y la
relacioacuten entre monoacutemeros (laacutectico glicoacutelico) de forma que un aumento del aacutecido glicoacutelico
acelera la peacuterdida de peso del poliacutemero debido a la mayor hidrofilicidad de la matriz (Makadia
and Siegel 2011)
Por otro lado se ha descrito una erosioacuten maacutes raacutepida de las microesferas con reduccioacuten en el
peso molecular de PLGA debido a la facilidad de penetracioacuten del agua y la posterior degradacioacuten
del poliacutemero (Blanco and Alonso 1998) Schrier y colaboradores trabajaron con microesferas
48
preparadas por WOW utilizando diferentes tipos de PLGA analizaron el importante papel del
peso molecular la relacioacuten laacutectico-glicoacutelico y los residuos de aacutecido (Schrier et al 2001) La
cantidad de rhBMP2 adsorbido en la superficie de la micropartiacutecula aumentoacute con la
hidrofobicidad del poliacutemero Al mismo tiempo la liberacioacuten estaba en correlacioacuten con el perfil
de degradacioacuten de los diferentes poliacutemeros (Schrier et al 2001)
Por lo tanto el uso de poliacutemeros maacutes hidroacutefilos reduce la interaccioacuten proteiacutena hidroacutefoba-
poliacutemero Este efecto favorece una distribucioacuten maacutes homogeacutenea en la matriz polimeacuterica y
aumenta la absorcioacuten de agua en las microesferas Por lo que la velocidad de liberacioacuten de
rhBMP2 encapsulada en microesferas compuestas por un copoliacutemero de di-bloque PEG-PLGA
se incrementa con el contenido de PEG de la matriz de poliacutemero (Lochmann et al 2010) Se
obtuvo un resultado similar utilizando copoliacutemeros tri-bloque PLGA-PEG-PLGA (White et al
2013) En este caso modificando la relacioacuten del monoacutemero (laacutectido-glicoacutelido) en el PLGA y
aumentando la cantidad de PLGA-PEG-PLGA en las formulaciones el patroacuten de liberacioacuten de
BMP-2 co-encapsulada con HSA en microesferas fue ajustable
Por otro lado el uso de mezclas de PLGA-poloxaacutemeros es uacutetil para obtener una liberacioacuten
sostenida durante maacutes de un mes sin incidencia de producirse una descarga inicial (drsquoAngelo et
al 2010) (Manuel J Santander-Ortega Lozano-Loacutepez et al 2010) Sin embargo para un
plaacutesmido encapsulado dentro de nanopartiacuteculas obtenidas mediante mezclas de PLGA-
poloxaacutemero la hidrofobicidad del surfactante permite prolongar la liberacioacuten hasta 2 semanas de
una manera controlada Por otra parte se alcanzoacute una liberacioacuten completa para la mezcla de
PLGA-poloxaacutemero en lugar de nanopartiacuteculas de PLGA en la que la liberacioacuten maacutexima fue de
alrededor del 40 (Csaba et al 2005)
Las mezclas de PLGA y poloxaacutemeros (pluronic F68) tambieacuten se pueden usar para obtener
vesiacuteculas o caacutemaras nanocompuestas mediante un proceso de doble emulsioacuten Estas vesiacuteculas son
adecuadas para la encapsulacioacuten de moleacuteculas hidrofoacutebicas e hidrofiacutelicas La presencia de
pluronic afecta la estabilidad coloidal de las vesiacuteculas y el patroacuten de liberacioacuten de las moleacuteculas
49
encapsuladas Estas vesiacuteculas presentan una pared de 30 nm y el faacutermaco estaacute encapsulado en
presencia del poloxaacutemero (Nair and Sharma 2012)
Otras estrategias incluyen el uso de diferentes compuestos para aumentar el tiempo de
liberacioacuten Por lo tanto BMP2 encapsulada en nanopartiacuteculas de PLGA-PVA (alrededor de 300
nm) mostroacute una mayor eficiencia de encapsulacioacuten y un perfil de liberacioacuten de corta duracioacuten con
un estallido inicial muy alto De forma similar las nanopartiacuteculas de mezcla PLGA-poloxaacutemero
se modificaron superficialmente introduciendo quitosano en el segundo paso de la siacutentesis Este
meacutetodo mostroacute un perfil de liberacioacuten sostenida de hasta 14 diacuteas sin ninguacuten estallido importante
inicial En este caso se utilizoacute un antiacutegeno recombinante de hepatitis B (Paolicelli et al 2010)
Ademaacutes el uso de heparina conjugada con microesferas porosas de PLGA tambieacuten se ha descrito
para obtener un sistema de administracioacuten a largo plazo que reduce al mismo tiempo el estallido
inicial En estos sistemas la heparina se inmovilizoacute en la superficie nano micropartiacutecula La
liberacioacuten se controloacute usando las afinidades de unioacuten de la heparina a varios factores de
crecimiento incluida la BMP2 En este caso el estallido inicial se redujo hasta un 4-7 durante
el primer diacutea seguido de una liberacioacuten sostenida de aproximadamente un 1 por diacutea (La et al
2010) (Chung et al 2007) (Jeon et al 2008)
La liberacioacuten de proteiacutena en la descarga inicial puede atenuarse mediante la fabricacioacuten de
microesferas de doble pared es decir micropartiacuteculas con nuacutecleo y concha La presencia de un
revestimiento o armazoacuten de PLA reduce la tasa de liberacioacuten de BSA encapsulado en el nuacutecleo
PLGA y extiende la duracioacuten del perfil de liberacioacuten hasta dos meses Por otra parte un aumento
en el peso molecular de PLA influye en la tasa de erosioacuten de las partiacuteculas lo que ralentiza auacuten
maacutes la liberacioacuten de proteiacutenas (Xia et al 2013)
La modificacioacuten de la viscosidad en el entorno de micropartiacuteculas influye adicionalmente en
el patroacuten de liberacioacuten La viscosidad puede controlar el estallido en el punto maacutes temprano y
promover una liberacioacuten sostenida Esta situacioacuten se ha demostrado para las microesferas de
rhBMP2-PLGA incrustadas en un hidrogel de aacutecido quitosano-tioglicoacutelico (Poloxaacutemero 407) (Fu
50
et al 2012) Yilgor y colaboradores tambieacuten incorporaron las nanopartiacuteculas de su sistema de
administracioacuten secuencial a un scaffolds compuesto por quitosano y quitosano-PEO (Yilgor et al
2009) En otro trabajo las microesferas de PLGA PVA con BMP2 encapsulado se combinaron
con diferentes biomateriales compuestos (hidrogel de gelatina o fumarato de polipropileno) La
liberacioacuten sostenida de la moleacutecula bioactiva se extendioacute durante un periacuteodo de 42 diacuteas Los
resultados in vivo indican la importancia de las caracteriacutesticas compuestas En este caso se obtuvo
una mejor formacioacuten de hueso cuando las micropartiacuteculas de PLGA se incorporaron a la matriz
maacutes hidroacutefoba (fumarato de polipropileno) (Kempen et al 2008) (Kempen et al 2009)
Finalmente la tabla 1 resume informacioacuten importante sobre diferentes paraacutemetros relacionados
con el uso de nano o micropartiacuteculas basadas en PLGA para encapsular transportar y liberar
factores de crecimiento (principalmente BMP2) La mayoriacutea de ellos estaacuten en la escala
microscoacutepica El PVA ha sido el estabilizador de surfactante maacutes utilizado Es posible encontrar
tanto la encapsulacioacuten como la adsorcioacuten de superficie de los factores de crecimiento con una
eficiencia alta a moderada El uso de heparina como estabilizador reduce significativamente la
liberacioacuten inicial en estallido favoreciendo una liberacioacuten sostenida en el tiempo La bioactividad
del GF se conservoacute en la mayoriacutea de los sistemas y la encapsulacioacuten con otras biomoleacuteculas parece
tener un efecto similar al del uso de surfactantes como estabilizadores
51
TABLA 1 Sistemas de nano micropartiacuteculas para encapsular GF principalmente el factor
de crecimiento BMP2
Poliacutemeros Estabilizadores Tamantildeo EE
Encapsulacioacuten Liberacioacuten
Actividad
bioloacutegica Referencia
PLGA PVA 10-20 microm Adsorcioacuten
rhBMP2
20 ngml de
contasnte
liberacioacuten
sostenida
Mejor formacioacuten
oacutesea 8 semanas
despueacutes
Fu at al 2012
(126)
PLGA PVA 10-100
μm
rhBMP2-BSA
69 (BMP)
Burst (20 )
Prolongado
hasta un 77
(28 diacuteas)
Moleacuteculas de
BMP2 con
bioactividad
Tian et al
2012
(130)
PLGA 7525 PVA 182 μm 82 -
Buenos resultados
de reparacioacuten oacutesea
de 8 a 12 semanas
Rodriguez-
Evora et al
2014 (130)
PLGA PVA 228 μm 605
30 en la
descarga inicial
Liberacioacuten maacutes
lenta de un 4
por semana
Despueacutes de 8
semanas
liberado un
60
Sin peacuterdida de
bioactividad
Reyes et al
2013 (132)
PLGAPEG Sin siacutentesis de
doble emulsion
100-200
μm Adsorcioacuten BMP2
13 en la
descarga inicial
Liberacioacuten mas
lenta de 001-8
por dia
Despueacutes de 23
diacuteas liberado
un 70
Sustancial
regeneracioacuten oacutesea
debido al
ldquoandamiordquo
Rahman et al
2014 (181)
PLGA Diferente PVA 20-100
μm
30 (sin cubrir
PLGA)
90 (cubriendo
PLGA)
26-49 (1 dia)
Total 2 semanas
despueacutes
Sin peacuterdida de
bioactividad
Lupu-Haber
et al 2013
(134)
PLGA 7525 PVA 5-125 μm -
Descarga inicial
de un 30 (1
diacutea)
Prolongada 35
diacuteas
Mayores
voluacutemenes y
cobertura de
superficie del
Nuevo hueso
Wink et al
2014
(138)
PLGA Heparina 200-800
nm
Adsorcioacuten BMP2
94
Sin descarga
inicial
Prolongado 4
semanas
Reduccioacuten
significativa de la
dosis de BMP2
para una Buena
formacioacuten oacutesea
La et al 2010
(122)
PLGA Heparina-
poloxaacutemero 160 nm
Adsorcioacuten BMP2
100
Descarga inicial
(4-7) perfil
lineal
Mayor
mineralizacioacuten de
la matriz del hueso
regenerado
Chung et al
2007 (123)
PLGA Heparina 100-250
nm Adsorcioacuten 94
Descarga inicial
10 (1 dia)
60 30 diacuteas
despueacutes
Sin peacuterdida de
bioactividad
Eficacia de la
administracioacuten
cantidad 50 veces
menor
Jeon et al
2008 (124)
PLGA PVA ~ 300 nm 80 85 descarga
inicial (1 dia)
Sin peacuterdida de
bioactividad
Yilgor et al
2009 (127)
PLGA (en anillos) PVA 215 μm 66 Descarga El 60 de los Rodriguez-
52
15 Vectorizacioacuten Entrega dirigida
Las nano-esferas de PLGA representan un sistema de administracioacuten de biomoleacuteculas bien
estudiado que podriacutea aplicarse a la seleccioacuten celular con el fin de mejorar el suministro de
proteiacutenas especiacuteficas o aacutecidos nucleicos dentro o cerca de las ceacutelulas de referencia de ingenieriacutea
oacutesea es decir ceacutelulas madre mesenquimales (Vo Kasper and Mikos 2012) Las propiedades de
direccionamiento pueden ser suministradas por una estrategia de funcionalizacioacuten del ligando
modificacioacuten de la estructura superficial del nano-transportador conjugando un ligando especiacutefico
de ceacutelula para dirigir la liberacioacuten de biomoleacuteculas encapsuladas preferiblemente en estrecha
asociacioacuten con las ceacutelulas diana (Ji et al 2012) El uso de nanopartiacuteculas con una unioacuten covalente
de diferentes ligandos da lugar a una teacutecnica potencial para administrar biomoleacuteculas especiacuteficas
de ceacutelulas oacuteseas para la ingenieriacutea oacutesea (Luginbuehl et al 2004)
Los anticuerpos especiacuteficos que reconocen los receptores de superficie en estas ceacutelulas podriacutean
acoplarse covalentemente a la superficie de las nanopartiacuteculas de PLGA obteniendo inmuno-
nanopartiacuteculas Hay varios ejemplos de inmovilizacioacuten de anticuerpos en la superficie de
nanopartiacuteculas de PLGA Kocbek y colaboradores demostraron el reconocimiento especiacutefico de
las ceacutelulas tumorales de mama por un anticuerpo monoclonal especiacutefico unido a las nanopartiacuteculas
fluorescentes PLGA obtenidas mediante el proceso de emulsioacuten WOW (Kocbek et al 2007)
Para la unioacuten covalente de la superficie utilizaron un meacutetodo de carbodimida maacutes simple que
promueve la formacioacuten de un enlace amida entre los grupos terminales carboxiacutelicos libres de
nanopartiacuteculas de PLGA y los grupos amino primarios de la moleacutecula del anticuerpo (Ertl et al
2000) Este procedimiento puede verse muy influenciado por la presencia de estabilizadores
frecuentemente utilizados para conferir estabilidad coloidal a las nanopartiacuteculas La movilidad
electroforeacutetica de las nanopartiacuteculas de PLGA con un anticuerpo (inmuno-γ-globulina anti-
proteiacutena C-reactiva humana) unido covalentemente a la superficie como se muestra en la figura
5 Es necesario observar la draacutestica disminucioacuten en los valores de movilidad del anticuerpo
modificado con respecto a las nanopartiacuteculas de PLGA desnudas lo que podriacutea implicar una baja
53
estabilidad coloidal y la posterior agregacioacuten del nanosistema Santander y colaboradores
propusieron una menor carga de anticuerpos en la que las nanoparticulas de PLGA desnudas
deben ser recubiertas por un agente surfactante no ioacutenico con el fin de obtener nanopartiacuteculas
estables inmunorrectivas (Santander-Ortega Bastos-Gonzalez and Ortega-Vinuesa 2007)
Ratzinger y colaboradores indicaron que la presencia de altas concentraciones de poloxaacutemero
disminuyoacute la eficacia de acoplamiento a grupos carboxiacutelicos en nanopartiacuteculas de PLGA lo que
demuestra que es necesario un equilibrio que combine maacutes estabilidad y mejor eficacia de
acoplamiento (Ratzinger et al 2010) Para evitar este problema Cheng y colaboradores
sintetizaron el copoliacutemero en bloque de PLGA-PEG funcionalizado con carboxilo uniendo un
aptaacutemero especiacutefico a la superficie de las nanopartiacuteculas pegiladas mediante el meacutetodo de la
carbodiimida En este trabajo se ha demostrado una mejor administracioacuten de faacutermacos en los
tumores de proacutestata en comparacioacuten con las nanopartiacuteculas equivalentes no dirigidas (Cheng et
al 2007)
16 Ingenieriacutea tisular soportes 3D o ldquoscaffoldsrdquo
Los datos publicados en la literatura indican que las nanopartiacuteculas micropartiacuteculas de PLGA
prometen lograr una administracioacuten sostenida espacial y temporalmente controlada por factores
de crecimiento requeridos para el desarrollo celular y la diferenciacioacuten celular Se pueden
incorporar a ceacutelulas en scaffolds soacutelidos o hidrogeles inyectables (Danhier et al 2012) Los
scaffolds o andamios son estructuras 3D porosas que normalmente se utilizan para mejorar la
ingenieriacutea tisular oacutesea (A C Carreira et al 2014) De acuerdo con Tian y colaboradores (Tian et
al 2012) un scaffolds disentildeado con este objetivo debe tener (1) resistencia mecaacutenica apropiada
para soportar el crecimiento de hueso nuevo (2) porosidad apropiada para permitir el crecimiento
de las ceacutelulas relacionadas con los huesos (3) buena biocompatibilidad que permite el crecimiento
de ceacutelulas en su superficie sin ser rechazado por el cuerpo (4) baja toxicidad para las ceacutelulas y
tejidos de alrededor (5) ser capaz de inducir la diferenciacioacuten osteogeacutenica de las ceacutelulas madre
54
relacionadas con los huesos (6) ser biodegradable con productos de degradacioacuten no toacutexicos para
que eventualmente puedan ser reemplazados por nuevo hueso Ademaacutes el scaffolds para la
regeneracioacuten oacutesea debe mantener el suministro o la liberacioacuten de BMP (factores de crecimiento)
in situ durante un tiempo prolongado De esta forma las nano micropartiacuteculas dentro de los
scaffolds se utilizan para liberar un flujo adecuado de estas biomoleacuteculas de sentildealizacioacuten y
preservar su estructura funcional (Romagnoli DrsquoAsta and Brandi 2013) Para hacerlo se requiere
una liberacioacuten inicial del factor de crecimiento encapsulado en las primeras horas para poder
obtener raacutepidamente una concentracioacuten terapeacuteutica efectiva seguida de un perfil de liberacioacuten
sostenido a largo plazo (Puppi et al 2014) La mayoriacutea de las partiacuteculas polimeacutericas insertadas
en las estructuras de los scaffolds estaacuten en una escala micromeacutetrica El objetivo principal de estas
micropartiacuteculas es la proteccioacuten y el control temporal de la entrega del factor de crecimiento Sin
embargo dada la porosidad de estas estructuras las nanopartiacuteculas y especialmente las partiacuteculas
de algunas micras pueden volverse maacutes importantes ya que es posible disentildear sistemas con una
difusioacuten simple y faacutecil a traveacutes de la estructura Este proceso podriacutea permitir el reconocimiento
especiacutefico de un tipo de ceacutelula particular liberando sus BMP encapsuladas en el mismo entorno
y ayudando a su diferenciacioacuten al tejido celular oacuteseo
55
2 HIPOacuteTESIS
Lo que sabemos hasta ahora es que las BMPs y especiacuteficamente la BMP-2 son muy uacutetiles
para promover la regeneracioacuten oacutesea induciendo una mayor formacioacuten de hueso de la zona
receptora de igual calidad que el hueso nativo del paciente Sin embargo la administracioacuten local
presenta algunas limitaciones como que la proteiacutena se puede inactivar muy raacutepidamente y la
distribucioacuten de la BMP en una suspensioacuten liacutequida hace que sea imposible estar seguro de que la
proteiacutena haya alcanzado el objetivo Para ayudar a resolver estos problemas planteamos la
siguiente hipoacutetesis
HIPOTESIS CIERTA La encapsulacioacuten de BMP-2 con nuestras nanopartiacuteculas permiten una
administracioacuten localizada un transporte especifico y una liberacioacuten controlada de la biomoleacutecula
mejorando asiacute su farmacocineacutetica y farmacodinamia y disminuyendo los efectos secundarios
derivados de esta
HIPOacuteTESIS NULA Que tras la experimentacioacuten no seamos capaces de promover la
encapsulacioacuten de la BMP-2 dentro de nuestras nanopartiacuteculas de PLGA o que producieacutendose no
se produzca la administracioacuten de BMP-2 localizada y liberacioacuten prolongada o que nuestro sistema
de encapsulacioacuten pueda presentar efectos citotoacutexicos a nivel celular
56
3OBJETIVOS
31 Objetivo principal
Optimizar la formulacioacuten de diferentes tipos de nanopartiacuteculas de PLGA para el transporte y
suministro de BMP-2 que permita conseguir una cineacutetica de liberacioacuten controlada aumentando la
vida media de este factor de crecimiento oacuteseo y preservando su accioacuten bioloacutegica
32 Objetivos secundarios
Nos planteamos los siguientes
1 Llevar a cabo la siacutentesis de NPs polimeacutericas de PLGA mediante el procedimiento de
doble emulsioacuten para conseguir nanosistemas coloidal y temporalmente estables
2 Modelar los procedimientos de siacutentesis de NPs de PLGA para la encapsulacioacuten o carga
de moleacuteculas proteicas en la proporcioacuten adecuada sin alterar su actividad bioloacutegica
3 Desarrollar una completa caracterizacioacuten quiacutemico-fiacutesica de los diferentes nanosistemas
polimeacutericos en ausencia de proteiacutena y con carga de la misma haciendo hincapieacute en la
interaccioacuten surfactante-proteiacutena y analizando sus propiedades superficiales y
coloidales
4 Desarrollar una caracterizacioacuten bioloacutegica de los diferentes nanosistemas con proteiacutena
centrada en el anaacutelisis de la cineacutetica de liberacioacuten proteica y su actividad bioloacutegica
5 Evaluar in vitro las interacciones celulares citotoxicidad y captacioacuten celular de los
diferentes sistemas de NPs de PLGA en ceacutelulas estromales mesenquimales de hueso
alveolar humano
6 Tomando como punto de partida el modelo de NPs con proteiacutena con las mejores
propiedades coloidales y bioloacutegicas formular las condiciones oacuteptimas para obtener un
nanotransportador de partiacuteculas de PLGA cargado con BMP2
57
7 Desarrollar una completa caracterizacioacuten quiacutemico-fiacutesica y bioloacutegica de las NPs con
BMP2 analizando sus propiedades superficiales coloidales y la cineacutetica de liberacioacuten
proteica
8 Analizar la actividad bioloacutegica de la BMP-2 vehiculizada mediante las NPs de PLGA
mediante experiencias de proliferacioacuten migracioacuten y diferenciacioacuten osteogeacutenica en
ceacutelulas estromales mesenquimales de hueso alveolar humano
58
4NANOSISTEMA DE ENTREGA DOBLE PARA BIOMOLEacuteCULAS
FORMULACIOacuteN CARACTERIZACIOacuteN Y LIBERACIOacuteN IN
VITRO
41Antecedentes
La regeneracioacuten tisular es una accioacuten bioloacutegica compleja que implica muacuteltiples pasos de forma
secuencial ordenada y controlada (Padial-Molina et al 2012) (Padial-Molina Rodriguez et al
2015) Claacutesicamente se han propuesto moleacuteculas bioactivas para ayudar en estos procesos Sin
embargo el uso de altas dosis la desnaturalizacioacuten y la peacuterdida de actividad bioloacutegica el tiempo
de accioacuten descontrolado y la difusioacuten a otros tejidos destacan como los principales problemas de
esta estrategia terapeacuteutica (Ortega-Oller et al 2015) Para ayudar a resolver estas dificultades en
los uacuteltimos antildeos se ha investigado intensamente la nanomedicina como un aacuterea emergente Esto
implica meacutetodos de diagnoacutestico terapeacuteuticos y de regeneracioacuten mediante estructuras y sistemas
en los que el tamantildeo y la forma se controlan a nivel atoacutemico molecular y supramolecular (Ki-
Bum Lee Ani Solanki J Dongun Kim 2009) El transporte y la administracioacuten controlada de
faacutermacos yo biomoleacuteculas terapeacuteuticas mejora su farmacocineacutetica y farmacodinaacutemica y al
mismo tiempo minimiza los efectos secundarios nocivos Para estos propoacutesitos se describieron
diferentes tipos de nanosistemas El aacutecido polilaacutectico-co-glicoacutelico (PLGA) exhibe una baja
citotoxicidad asi como una alta biocompatibilidad y biodegradabilidad con las liberaciones de
subproductos no toacutexicos
En la uacuteltima deacutecada se ha investigado el uso de PLGA para administrar un amplio espectro de
agentes activos desde moleacuteculas de faacutermacos hidroacutefobas (Yallapu et al 2010) (Nair and Sharma
2012) (Shankarayan Kumar and Mishra 2013) a biomoleacuteculas hidroacutefilas como peacuteptidos
(Loureiro et al 2016) proteiacutenas (Blanco and Alonso 1998) (Perez De Jesus and Griebenow
2002) (Manuel J Santander-Ortega Csaba et al 2010) (Pirooznia et al 2012) (drsquoAngelo et al
2010) (White et al 2013) o aacutecidos nucleicos (Pantazis et al 2012) (Park et al 2013) Estos
59
sistemas de entrega se han producido a traveacutes de diferentes procesos de formulacioacuten para su
aplicacioacuten en terapias tanto sisteacutemicas como locales especiacuteficas del sitio (Wan and Yang 2016)
Sin embargo su disentildeo y desarrollo como nanotransportadores son difiacuteciles debido al patroacuten
de liberacioacuten problemaacutetico que presentan cuando las moleacuteculas encapsuladas son proteiacutenas para
las cuales las descargas iniciales y la liberacioacuten lenta o incompleta podriacutean ser un problema (Wan
and Yang 2016) (Giteau et al 2008) (Fredenberg et al 2011) Ademaacutes las condiciones
especiacuteficas de la liberacioacuten pueden necesitar ser diferentes dependiendo de la aplicacioacuten final del
nanotransportador (Fredenberg et al 2011) (Mohamed and van der Walle 2008)
La teacutecnica de doble emulsioacuten de aguaaceiteagua (WOW) es el meacutetodo de encapsulacioacuten de
proteiacutenas maacutes ampliamente utilizado para PLGA en micro (MP) y nanopartiacuteculas (NP) (Csaba et
al 2004) (Makadia and Siegel 2011) Permite modular diferentes factores como el tipo de PLGA
el uso de otros poliacutemeros mezclados con PLGA la adicioacuten de surfactantes el estreacutes mecaacutenico o
el solvente orgaacutenico (Fredenberg et al 2011) Tambieacuten es posible construir varios tipos de
copoliacutemeros para modificar la hidrofobicidad la relacioacuten de hidrofilicidad (Wan and Yang 2016)
(Danhier et al 2012) y la estabilidad el tamantildeo y el proceso de liberacioacuten coloidal El par PLGA
polietilenglicol y los surfactantes como el alcohol poliviniacutelico (PVA) o los oacutexidos de polietileno
(PEO) son los maacutes ampliamente estudiados (Nair and Sharma 2012) (Manuel J Santander-
Ortega Csaba et al 2010) (Ratzinger et al 2010) (Meng et al 2003)
Por otro lado la ingenieriacutea de tejidos requiere la participacioacuten de ceacutelulas estromales
mesenquimales (MSC) (Padial-Molina OrsquoValle et al 2015) Se sabe que las MSC tienen la
capacidad de diferenciarse en muacuteltiples tipos de ceacutelulas incluidos los osteoblastos Los
osteoblastos son las ceacutelulas principales responsables de sintetizar el tejido oacuteseo mineralizado Este
proceso estaacute regulado por entre otras moleacuteculas BMP-2 (Ortega-Oller et al 2015) Las
partiacuteculas de PLGA cargadas con BMP-2 son sistemas ampliamente utilizados como se ha
descrito y revisado por otros autores (Ortega-Oller et al 2015) (Yilgor Hasirci and Hasirci 2010)
(Li et al 2009) (Wang et al 2015) (Shim et al 2016)
60
Asiacute dentro de este contexto el objetivo del presente estudio fue optimizar la formulacioacuten y
propiedades de un sistema de nanopartiacuteculas con gran variedad de aplicaciones terapeacuteuticas
Probamos dos estrategias diferentes para obtener NP de tensioactivo PLGA utilizando lisozima
como modelo para BMP-2 Analizamos el tamantildeo y la morfologiacutea el iacutendice de polidespersidad
el potencial zeta la estabilidad coloidal y la eficiencia de encapsulacioacuten (EE) de la proteiacutena
Una vez finalizada la caracterizacioacuten fiacutesico-quiacutemica el estudio se centroacute en el proceso de
liberacioacuten de proteiacutenas utilizando diferentes teacutecnicas para estudiar los resultados de experimentos
in vitro y centraacutendose en el patroacuten de liberacioacuten y la actividad bioloacutegica de la lisozima liberada
De esta manera se establecioacute una nueva formulacioacuten para desarrollar un nanosistema de PLGA
con una distribucioacuten de tamantildeo dual singular y el equilibrio adecuado entre encapsulacioacuten y
liberacioacuten de proteiacutenas bioloacutegicamente activas Finalmente los efectos del sistema PLGA
propuesto se probaron en MSC primarias in vitro como prueba del nuevo sistema desarrollado
42Materiales y meacutetodos
421Formulacioacuten de las nanoparticulas
El aacutecido poli (laacutectico-co-glicoacutelico) (PLGA 5050) ([C2 H2 O2]x [C3 H4 O2]y) x = 50 y = 50
(Resomerreg 503H) 32 - 44 kDa se usoacute como poliacutemero El surfactante polimeacuterico Pluronicreg F68
(Poloxaacutemero 188) (Sigma-Aldrich) se usoacute como el emulsionante Su estructura se basa en un
copoliacutemero tri-bloque de poli (oacutexido de etileno) PEO y poli (oacutexido de propileno) PPO (PEO)a ndash
(PPO)b ndash (PEO)a con a = 75 y b = 30 La lisozima de huevo de gallina (Sigma-L7651) se usoacute
como proteiacutena hidroacutefila El agua se purificoacute en un sistema Milli-Q Academic de Millipore Se
desarrollaron dos meacutetodos de formulacioacuten diferentes denominados O-F68 y W-F68
En el meacutetodo O-F68 se disolvieron 25 mg de PLGA y 15 mg de F68 en 660 120583119871 de
diclorometano (DMC) y se agitaron en el vortex Luego se antildeadieron 330 micro-litros de acetona
y se agitaron en el vortex tambieacuten A continuacioacuten se antildeadieron 100 120583119871 de una solucioacuten
tamponada a pH 128 con o sin lisozima (5 mg mL) gota a gota mientras se agita en vortex
61
durante 30 s Inmediatamente esta emulsioacuten primaria de agua aceite (WO) se vertioacute en un vidrio
que conteniacutea 125 ml de etanol bajo agitacioacuten magneacutetica y se antildeadieron 125 ml de agua MilliQ
Despueacutes de 10 minutos de agitacioacuten magneacutetica los disolventes orgaacutenicos se extrajeron
raacutepidamente por evaporacioacuten al vaciacuteo hasta que la muestra alcanzoacute un volumen final de 10 ml
En el meacutetodo W-F68 se disolvieron 100 mg de PLGA en un tubo que conteniacutea 1 ml de acetato
de etilo (EA) y se agitaron en vortex Se antildeadieron 40 120583119871 de una solucioacuten tamponada a pH 128
con o sin lisozima (20 mg ml) e inmediatamente se sonicaron (Branson Ultrasonics 450 Analog
Sonifier) fijando el debido ciclo de trabajo al 20 y de control de salida a 4 durante 1 min con
el tubo rodeado de hielo Esta emulsioacuten primaria WO se vertioacute en un tubo de plaacutestico que conteniacutea
2 ml de una solucioacuten tamponada (pH 128) de F68 a 1 mg ml y se agitoacute en voacutertex durante 30 s
Luego el tubo rodeado de hielo se sonicoacute de nuevo a la maacutexima amplitud para la micro punta
(control de salida 7) durante 1 minuto Esta segunda emulsioacuten WOW se vertioacute en un vaso que
conteniacutea 10 ml de la solucioacuten tamponada de F68 y se mantuvo bajo agitacioacuten magneacutetica durante
2 min El disolvente orgaacutenico se extrajo luego raacutepidamente por evaporacioacuten al vaciacuteo hasta un
volumen final de 8 ml
422 Limpieza y almacenamiento
Despueacutes de la evaporacioacuten del disolvente orgaacutenico la muestra se centrifugoacute durante 10 min a
20 deg C a 14000 o 12000 rpm para los meacutetodos O-F68 y W-F68 respectivamente El sobrenadante
se filtroacute usando filtros de 100 nm para medir la proteiacutena no encapsulada libre El sedimento se
resuspendioacute luego en PB hasta un volumen final de 4 ml y se mantuvo en refrigeracioacuten a 4ordmC
1La carga de proteiacutenas y la eficiencia de encapsulacioacuten
La carga de proteiacutena inicial se optimizoacute para la formulacioacuten de nanopartiacuteculas preservando la
estabilidad coloidal final despueacutes del paso de evaporacioacuten y siendo diferente para cada
nanosistema Ademaacutes se usoacute 16 p p (Lys PLGA) para O-F68 y 08 p p (Lys PLGA)
para W-F68 La cantidad de lisozima encapsulada se calculoacute midiendo la diferencia entre la
cantidad inicial antildeadida y la proteiacutena libre no encapsulada que se analizoacute mediante el ensayo con
62
aacutecido bicinconiacutenico (BCA Sigma-Aldrich) Luego la eficacia de encapsulacioacuten de proteiacutenas (EE)
y la carga de faacutermaco final (DL) se calcularon de la siguiente manera
EE = 119872119868minus119872119865
119872119868119909 100 119863119871 =
119872119868minus119872119865
119872119868119901119900119897119894119898119890119903 119909 10
donde MI es la masa total inicial de Lys MF es la masa total de Lys en el sobrenadante acuoso
y Mpoliacutemero es la masa de PLGA en la formulacioacuten
423 Caracterizacioacuten de las nanoparticulas
2Caracterizacioacuten interfacial de la primera emulsioacuten agua en aceite
La tensioacuten superficial y las mediciones de reologiacutea dilatacional en la interfaz aire-agua se
realizaron en el OCTOPUS (Maldonado-Valderrama et al 2013) dispositivo de anaacutelisis de
superficies por gota pendiente con intercambio muacuteltiple de subfase (patente presentada
P201001588) descrito en detalle por Cabrerizo-Viacutelchez y col (Wege Holgado-Terriza and
Cabrerizo-Vilchez 2002) Aquiacute el aire juega el papel de la fase orgaacutenica La tensioacuten superficial
se calcula con el software DINATENreg basado en el anaacutelisis de forma de gota axisimeacutetrica
(ADSA) y el moacutedulo de dilatacioacuten (E) de la capa interfacial se determina a partir del anaacutelisis de
imagen con el programa CONTACTOreg
3Morfologiacutea de la partiacutecula
Las nanopartiacuteculas se obtuvieron por microscopiacutea electroacutenica de barrido (SEM) y microscopiacutea
electroacutenica de transmisioacuten de barrido (STEM) usando un microscopio electroacutenico de barrido de
emisioacuten de campo Zeiss SUPRA 40VP del Centro de Instrumentacioacuten Cientiacutefica de la Universidad
de Granada (CIC UGR)
4Tamantildeo de las nanoparticulas y movilidad electrocineacutetica
El diaacutemetro hidrodinaacutemico y la movilidad electroforeacutetica de las NP se determinaron usando un
dispositivo Zetasizer NanoZeta ZS (Malvern Instrument Ltd UK) que trabaja a 25 deg C con un
63
laacuteser He-Ne de 633 nm y un aacutengulo de dispersioacuten de 173 deg Cada punto de datos se tomoacute como un
promedio de tres mediciones de muestra independientes El tamantildeo de las NP se caracterizoacute por
escaacutener de luz dinaacutemica (DLS) Se calcularon el diaacutemetro hidrodinaacutemico promedio (media Z o
media acumulada) y el iacutendice de polidispersidad (PDI) Estos paraacutemetros se calculan a traveacutes de
un anaacutelisis acumulativo de los datos que es aplicable para las distribuciones de tamantildeo
monomodal estrecho (Hassan Rana and Verma 2015) Tambieacuten determinamos la distribucioacuten
del tamantildeo de intensidad a partir de un algoritmo proporcionado por el software Zetasizer
La movilidad electroforeacutetica se determinoacute mediante la teacutecnica de electroforesis laacuteser Doppler
Se establecioacute una distribucioacuten de movilidad electroforeacutetica asiacute como una movilidad
electroforeacutetica promedio para cada muestra (entendiendo por promedio dos veces seguidas para
cada una de las muestras)
La distribucioacuten del tamantildeo hidrodinaacutemico de las NP con distribuciones de tamantildeo amplio de
DLS tambieacuten se midioacute usando anaacutelisis de seguimiento de nanopartiacuteculas (NTA) en un NanoSight
LM10-HS (GB) FT14 (NanoSight Amesbury Reino Unido) Todas las muestras se midieron maacutes
de tres veces durante 60 s con ajuste manual del obturador ganancia brillo y umbral a 25 deg C La
distribucioacuten de tamantildeo promedio (concentracioacuten de partiacuteculas frente a diaacutemetro) se calculoacute como
un promedio de al menos tres independientes distribuciones del tamantildeo
5Resonancia magneacutetica nuclear (RMN) de las nanopartiacuteculas
El espectro de 1HNMR de F68 libre las partiacuteculas cargadas con lisozima del meacutetodo O-F68
con y sin F68 y las partiacuteculas cargadas con lisozima del meacutetodo W-F68 se midieron con un
espectroacutemetro VNMRS de 500 MHz (Agilent) en el Centro de Instrumentacioacuten Cientiacutefica (CIC)
de la Universidad de Granada
424 Estabilidad coloidal y temporal en biologiacutea media
Se midioacute el diaacutemetro hidrodinaacutemico promedio y el iacutendice de polidispersidad (PDI) por DLS de
cada sistema para determinar su estabilidad coloidal en diferentes medios (tampoacuten de fosfato
64
[PB] solucioacuten salina tamponada con fosfato [PBS] y medio de cultivo celular medio de Eagle
modificado de Dulbecco [DMEM] de Sigma) y en diferentes momentos despueacutes (0 1 y 5 diacuteas)
Los experimentos de liberacioacuten in vitro se realizaron siguiendo una metodologiacutea similar a la
descrita anteriormente (eficacia de encapsulacioacuten) pero utilizando 1 ml de cada muestra
suspendida en PBS a 37 C La proteiacutena liberada de estas muestras se determinoacute cada 24 horas
mediante anaacutelisis de sobrenadante y el sedimento se suspendioacute en el mismo volumen de tampoacuten
para mantener las condiciones de liberacioacuten Todos los experimentos fueron desarrollados por
triplicado
6Microscopia confocal
La lisozima se marcoacute con isotopo de fluoresceiacutena (FITC) usando un meacutetodo descrito por Kok
et al (Kok et al 1998) Despueacutes de la conjugacioacuten covalente de FITC y lisozima las
concentraciones se estimaron espectrofotomeacutetricamente utilizando los coeficientes de extincioacuten
descritos para FITC a 494 nm y 280 nm La concentracioacuten de lisozima se calculoacute midiendo la
absorbancia oacuteptica a 280 nm y restando la absorbancia FITC correspondiente a esta longitud de
onda Las imaacutegenes se realizaron en un microscopio confocal de escaneo laacuteser Nikon A1 de CIC
UGR Todos los experimentos se realizaron por triplicado y se replicaron al menos dos veces
425 Actividad bioloacutegica e interacciones
7Actividad bioloacutegica de la lisozima
La actividad bioloacutegica de la lisozima se analizoacute mediante un kit de actividad enzimaacutetica
(Sigma-Aldrich) utilizando ceacutelulas de Micrococcus lysodeikticus como sustrato siguiendo las
instrucciones del fabricante
8Captacioacuten celular
Se tomaron ceacutelulas madre mesenquimales humanas primarias (hMSC) del hueso alveolar
maxilar sano de acuerdo con protocolos descritos (Mason et al 2014) Despueacutes de confirmar su
fenotipo por pruebas de citometriacutea de flujo y diferenciacioacuten trilinaje 12000 ceacutelulas por pozo se
cultivaron en placas esteacuteriles con fondo de vidrio (Ibidi cat n 81158) durante la noche Estas
65
ceacutelulas fueron tratadas con medio sin suero fetal bovino (FBS) y guiacutea celular roja (1 5000)
(C34552 ThermoFisher) durante 30 min Entonces el medio fue eliminado y complementado con
10 de SFB despueacutes de lo cual se agregaron partiacuteculas con lisozima-FITC Entonces los hMSCs
eran incubadas 30 minutos nuevamente lavadas tres veces con PBS 1X y un suplementado medio
fresco con 2 de FBS agregado Finalmente las hMSCs fueron examinadas por un microscopio
confocal (Nikon Eclipse Ti-E) Cultivo celular en todos los casos se mantuvieron a 37 deg C y 5
de atmoacutesfera de CO
43 Resultados y discusioacuten
431 Formulacioacuten de las nanoparticulas
Los meacutetodos desarrollados en este trabajo estaacuten destinados a mejorar las teacutecnicas de
formulacioacuten existentes para las NP de PLGA cargadas de proteiacutenas hidrofiacutelicas basadas en un
proceso de doble emulsioacuten (Blanco and Alonso 1998) (Csaba et al 2004) La novedad de estos
meacutetodos es el uso del surfactante polimeacuterico F68 ya sea en la fase orgaacutenica (meacutetodo O-F68) o en
la fase acuosa (W-F68) Este surfactante reduce el tamantildeo de las NP mejora su estabilidad y
protege la proteiacutena encapsulada Ademaacutes la presencia de F68 en la superficie de las partiacuteculas
reduce el reconocimiento de los nanovehiacuteculos (nanotransportadores) por el sistema mononuclear
fagociacutetico (MPS) (Farace et al 2016)
Ademaacutes la eleccioacuten del solvente orgaacutenico afecta significativamente las propiedades del
sistema coloidal final ya que la solubilidad del solvente orgaacutenico regula la estructura interna y
superficial de la partiacutecula Ademaacutes la interaccioacuten del disolvente con la biomoleacutecula encapsulada
puede alterar su bioactividad como consecuencia de su desnaturalizacioacuten como se encontroacute para
el cloruro de metileno (Meng et al 2003) En el meacutetodo O-F68 se elige DMC como disolvente
orgaacutenico debido a su menor solubilidad en agua para facilitar el proceso de emulsificacioacuten y su
bajo punto de ebullicioacuten para facilitar la evaporacioacuten Sin embargo se antildeadioacute un solvente orgaacutenico
libremente miscible en agua (acetona) y el emulsionante F68 en esta fase orgaacutenica para reducir
66
sus efectos bioloacutegicos negativos sobre la proteiacutena encapsulada (Danhier et al 2012) Este
emulsionante tambieacuten reduce la interaccioacuten de la matriz de PLGA proteiacutena-hidrofoacutebica y por lo
tanto la interrupcioacuten de la estructura de la proteiacutena (Ortega-Oller et al 2015) Por el contrario
en el meacutetodo W-F68 se utilizoacute acetato de etilo como disolvente orgaacutenico que ejerce menos
efectos de desnaturalizacioacuten sobre la proteiacutena encapsulada (Sturesson and Carlfors 2000) La
mayor solubilidad en agua de este solvente favorece la eliminacioacuten raacutepida del solvente La
velocidad de eliminacioacuten del disolvente tambieacuten se acelera al aumentar la tensioacuten de cizallamiento
durante la segunda etapa de emulsificacioacuten Tambieacuten mejora la eficacia del encapsulamiento y
minimiza el tiempo de contacto entre la proteiacutena y el disolvente orgaacutenico (Ortega-Oller et al
2015) El poloxaacutemero F68 se introduce en la fase acuosa externa
Ambas formulaciones (O-F68 y W-F68) (Tabla 2) dieron lugar a muestras coloidalmente
estables y a la encapsulacioacuten de la lisozima dentro de las nanopartiacuteculas de acuerdo con el doble
meacutetodo de emulsioacuten WOW (Makadia and Siegel 2011)
67
PLGA
(mg)
F68
(mg)
LYSI
(mg)
Initial EE
LYSF
(mg)
DL
O-F68-Lys 25 15 04 16 625 025 1
W-F68-Lys 100 2 08 08 731 058 058
Tabla 2 Condiciones de formulacioacuten y resultados de encapsulacioacuten de proteiacutenas PLGA F68
y LYSI son la cantidad inicial de poliacutemero surfactante y lisozima respectivamente El inicial
es la tasa inicial de proteiacutena-poliacutemero en pesopeso EE es la eficiencia de encapsulacioacuten LYSF
es la cantidad final encapsulada de lisozima DL es la tasa final de carga del faacutermaco en
pesopeso
La lisozima fue elegida como una proteiacutena modelo debido a su bioestabilidad sus
caracteriacutesticas bien conocidas y su facilidad para cuantificar su actividad bioloacutegica (Lin et al
2007) (Cai et al 2008) Ademaacutes su tamantildeo molecular (143 kD) y su punto isoeleacutectrico baacutesico
(alrededor de pH = 11) lo convierten en un modelo apropiado para otras proteiacutenas como los
factores de crecimiento oacuteseo (White et al 2013) Tres objetivos principales impulsaron la
optimizacioacuten de la relacioacuten apropiada entre el poliacutemero el poloxaacutemero y la proteiacutena (1) para
tener nanosistemas coloides estables de tamantildeos submicromeacutetricos (2) encapsular una cantidad
suficiente de proteiacutena y (3) para prevenir la desestabilizacioacuten de proteiacutenas manteniendo su
actividad bioloacutegica
Por lo tanto independientemente del meacutetodo de formulacioacuten se pretendiacutea limitar la carga de
proteiacutena inicial para proporcionar nanosistemas estables coloidalmente En nuestro caso como se
muestra en la Tabla 2 los valores de iniciales fueron la mejor opcioacuten para mantener la
estabilidad coloidal sin cambiar significativamente la distribucioacuten del tamantildeo (ver a
continuacioacuten) En consecuencia DL presenta valores relativamente bajos para ambas
formulaciones aunque la cantidad encapsulada de lisozima LYSF es mayor que las requeridas
68
para proteiacutenas terapeacuteuticas con cantidades cliacutenicamente efectivas maacutes bajas (Paillard-Giteau et
al 2010) El valor de EE encontrado para las NP de O-F68-Lys estaacute en consonancia con las
caracteriacutesticas de la formulacioacuten y es similar a otros informes con diferentes proteiacutenas (Manuel J
Santander-Ortega Csaba et al 2010) (Blanco and Alonso 1998) (Santander-Ortega et al 2009)
(drsquoAngelo et al 2010) incluida la albuacutemina de suero bovino (BSA) o la insulina (Manuel J
Santander-Ortega Csaba et al 2010) (Santander-Ortega et al 2009) y varios factores de
crecimiento (drsquoAngelo et al 2010)
La presencia de surfactante estabiliza la emulsioacuten y reduce su tamantildeo Sin embargo tambieacuten
altera la interaccioacuten proteiacutena-poliacutemero lo que se traduce en una reduccioacuten de la eficacia de
encapsulacioacuten Esto fue evidenciado por Blanco y colaboradores al encapsular BSA y lisozima en
diferentes micropartiacuteculas de PLGA-poloxaacutemero (Blanco and Alonso 1998) Ademaacutes el tipo de
proteiacutena y su carga teoacuterica inicial son factores directamente relacionados con la EE y pueden
afectar la estabilidad coloidal de la emulsioacuten primaria como lo muestran Santander y
colaboradores (Manuel J Santander-Ortega Csaba et al 2010) La diferente relacioacuten poliacutemero
tensioactivo entre las dos formulaciones no es comparable ya que el tensioactivo se agrega de una
manera diferente En ambos casos utilizamos formulaciones anteriores como punto de partida
(Blanco and Alonso 1998) (Csaba et al 2004) y probamos varias relaciones poliacutemero
tensioactivo (datos no mostrados) con el fin de obtener la mejor estabilidad coloidal EE y DL
En la Tabla 2 mostramos los datos para las relaciones optimizadas de PLGA F68 en ambos
sistemas
En el meacutetodo W-F68 a pesar del mayor valor de EE con respecto al sistema O-F68 se esperaba
una encapsulacioacuten casi completa debido a la baja relacioacuten inicial de proteiacutena masa de PLGA
(Manuel J Santander-Ortega Csaba et al 2010) y a la ausencia de surfactante en la primera
emulsioacuten Las caracteriacutesticas del proceso de formulacioacuten modificado pueden tener la clave En
esta formulacioacuten la solubilidad relativamente alta del acetato de etilo en agua promueve la
difusioacuten raacutepida del disolvente orgaacutenico en la segunda fase acuosa Inicialmente se agrega un
69
pequentildeo volumen inicial de agua que contiene poloxaacutemero para evitar una precipitacioacuten raacutepida e
incontrolada del poliacutemero y para controlar la velocidad del proceso Esto se complementa
posteriormente con la adicioacuten de un volumen acuoso maacutes grande como se describioacute anteriormente
(Meng et al 2003) Cuando esta solidificacioacuten es lenta favorece el escape de la proteiacutena y la EE
disminuye Sin embargo si la solidificacioacuten es muy raacutepida el contacto de la proteiacutena con el
solvente orgaacutenico se minimiza y la EE aumenta En el lado negativo puede producir aglomeracioacuten
de poliacutemero que interfiere con la correcta formacioacuten de las NP La introduccioacuten de un paso
intermedio con un volumen reducido de fase acuosa con poloxaacutemero puede modular la velocidad
del proceso controlando la difusioacuten de acetato de etilo en el agua y permitiendo la difusioacuten en la
fase orgaacutenica del poloxaacutemero Una velocidad controlada del proceso de pre-solidificacioacuten del
poliacutemero en presencia de surfactante puede producir canales o poros en la cubierta polimeacuterica
que por un lado podriacutean facilitar la liberacioacuten de proteiacutena y por otro lado podriacutea reducir el valor
EE (Rosca Watari and Uo 2004) Como resultado de estos fenoacutemenos los DL finales (p p de la
lisozima poliacutemero) que se muestran en la Tabla 2 para ambos sistemas NP son adecuados para
su aplicacioacuten como sistemas de nanotransporte
432 Caracterizacioacuten de las Nanopartiacuteculas
9Caracterizacioacuten interfacial de la primera formulacioacuten de agua en aceite
Para obtener una mejor comprensioacuten del efecto del meacutetodo de formulacioacuten sobre las
propiedades interfaciales de la primera solucioacuten de agua (solucioacuten de lisozima) emulsioacuten en
aceite disentildeamos experimentos de superficie con lisozima y Pluronicreg F68 La diferencia
principal en los dos meacutetodos de formulacioacuten es coacutemo se agrega Pluronicreg F68 en fase acuosa
(W-F68) o en fase orgaacutenica (O-F68) Esta diferencia podriacutea afectar la composicioacuten de la superficie
de las NP y como resultado sus propiedades coloidales
La tensioacuten superficial y la elasticidad en el interfaz aire-agua fueron las propiedades analizadas
(Tabla 3) En esta interfaz las proteiacutenas cambian su conformacioacuten y exponen su parte hidrofoacutebica
al aire dependiendo de su estabilidad termodinaacutemica flexibilidad anfipaticidad tamantildeo
70
molecular y carga En nuestro caso la lisozima es una proteiacutena globular que se adsorbe en la
interfase aire-agua y forma una monocapa riacutegida debido a su estructura interna y la presencia y
cantidad de puentes disulfuro (Pezennec et al 2008) Nuestras mediciones se realizaron a pH 12
por lo tanto la lisozima estaacute cargada negativamente La Tabla 3 muestra la tensioacuten interfacial de
la monocapa de lisozima en la interfaz aire-agua despueacutes de 50 minutos de adsorcioacuten (457 plusmn 04
(mN m)) y su elasticidad (83 plusmn 4 (mN m)) La reduccioacuten de la tensioacuten interfacial en comparacioacuten
con la de la interfaz aire-agua (72 mN m) indica las caracteriacutesticas de tensioactivo de la lisozima
El alto valor de la elasticidad se debioacute a la carga y a las altas interacciones moleculares en la
monocapa de lisozima Cuando la monocapa se forma con Pluronicreg F68 la tensioacuten superficial
es ligeramente menor que con la lisozima cuando se agrega Pluronicreg en AP pero similar
(teniendo en cuenta el error) cuando se agrega en OP
Pluronicreg F68 es una moleacutecula desmontable hiacutebrida que se introduce en el interfaz aire-agua
cuando se disuelve en fase acuosa y tambieacuten cuando se deposita en la superficie de la partiacutecula
Se encuentran pequentildeas diferencias al comparar la tensioacuten superficial de la monocapa Pluronicreg
de los dos meacutetodos Los diferentes valores de tensioacuten interfacial alcanzados en ambos casos se
deben a los diferentes meacutetodos para agregar Pluronicreg F68 a la monocapa de lisozima formada
Pluronicreg F68 presenta una elasticidad menor que la lisozima como se esperaba ya que se sabe
que Pluronicreg F68 forma una monocapa flexible en la interfaz aire-agua (Torcello-Goacutemez et al
2011)
71
Primer Paso
Tensioacuten
Interfacial
(mNm)
Elasticidada
(mNm)
Segundo
Paso
Tensioacuten
Interfacial
(mNm)
Elasticidadb
(mNm)
Lisozima 457plusmn04 83plusmn4 - - -
Lisozima 457plusmn04 83plusmn4 - - -
Pluronicreg
F68 (AP) 421plusmn03 15plusmn3
Pluronicreg
F68 (AP) 379plusmn06 142plusmn05
Pluronicreg
F68 (OP) 475plusmn21 94plusmn05
Pluronicreg
F68 (OP) 38plusmn2 43plusmn4
Tabla 3 Tensioacuten interfacial y elasticidad dilatacional (a 1 Hz) de la interfase aire-agua (a)
despueacutes de adsorber lisozima o Pluronic F68 en la fase acuosa (AP) o Pluronicreg F68 en fase
orgaacutenica (OP) en el primer paso (b) cuando Pluronic F68 se agrega en AP u OP despueacutes de la
adsorcioacuten de la monocapa de lisozima (media plusmn sd n = 3)
Se disentildearon dos ensayos para imitar los meacutetodos de formulacioacuten de las partiacuteculas En el primer
ensayo (meacutetodo W-F68) se formoacute una monocapa de lisozima luego la mayor parte de la
partiacutecula se intercambioacute con la solucioacuten acuosa de Pluronicreg F68 y despueacutes de la adsorcioacuten se
midieron la tensioacuten interfacial y la elasticidad del interfaz (379 plusmn 06 mN my 142 plusmn 05 mN
m respectivamente) Este bajo valor de elasticidad fue muy similar al de la monocapa de
Pluronicreg F68 lo que indica que Pluronicreg F68 se encuentra en el interfaz adecuado y elimina
la lisozima previamente adsorbida En el segundo ensayo (meacutetodo O-F68) despueacutes de que se
formara la monocapa de lisozima Pluronicreg F68 disuelto en cloroformo se depositoacute sobre la
superficie de la partiacutecula El cloroformo se evapora raacutepidamente y se mide la tensioacuten interfacial y
la elasticidad del interfaz (38 plusmn 2 mN my 43 plusmn 4 mN m respectivamente) La elasticidad fue la
mitad de la de la monocapa de lisozima pura tal vez debido a la coexistencia de las moleacuteculas de
72
lisozima y Pluronicreg F68 en el interfaz La tensioacuten superficial del interfaz final no depende del
meacutetodo de adicioacuten de Pluronicreg pero es menor que la de la lisozima pura o que el Pluronicreg
puro
Dentro de este contexto se ha informado ampliamente que la adsorcioacuten de PEO y poloxaacutemeros
en el interfaz reduce la unioacuten a proteiacutenas (Manuel J Santander-Ortega Lozano-Loacutepez et al
2010) (Torcello-Goacutemez et al 2011) En el meacutetodo O-F68 la lisozima se expone al DCM despueacutes
de la formacioacuten de la primera emulsioacuten de agua en aceite incluso si se agrega Pluronicreg ya que
ambos coexisten en el interfaz En el meacutetodo W-F68 la proteiacutena estaraacute en contacto con el acetato
de etilo en este paso ya que el Pluronicreg estaacute ausente Sin embargo este disolvente tiene efectos
bioloacutegicos maacutes deacutebiles sobre la lisozima Pluronicreg podriacutea alcanzar el interfaz cuando se agrega
a la fase acuosa en el siguiente paso y desplazar la proteiacutena del interfaz que podriacutea difundirse
hacia afuera a la fase acuosa
10Morfologiacutea de la partiacutecula
La entrega la biodistribucioacuten y el mecanismo de accioacuten de un faacutermaco o biomoleacutecula
transportada dependen en gran medida del tamantildeo de la partiacutecula la concentracioacuten y el tiempo
(Penaloza et al 2017) En general la escala micromeacutetrica estaacute disentildeada para un suministro local
que permite la formacioacuten de reservorios de la moleacutecula transportada y minimiza la accioacuten del
sistema fagociacutetico (Schwendeman et al 2014) Sin embargo los sistemas nanomeacutetricos son maacutes
versaacutetiles porque permiten una distribucioacuten sisteacutemica son maacutes estables reactivos y permiten la
accioacuten extra e intracelular Este uacuteltimo mecanismo es esencial cuando la moleacutecula o el faacutermaco
debe actuar en el citoplasma (Wang et al 2012) o en cualquier otra estructura intracelular como
la mitocondria el aparato de Golgi el retiacuteculo endoplaacutesmico o el nuacutecleo (Penaloza et al 2017)
(Vasir and Labhasetwar 2007) (Yameen et al 2014) Tambieacuten se han investigado otros
paraacutemetros para alterar el destino intracelular de las partiacuteculas principalmente alterando la
decoracioacuten de su superficie (Sneh-Edri Likhtenshtein and Stepensky 2011) por ejemplo con
sentildeales de localizacioacuten nuclear (NLS) que utilizan el nuacutecleo como el objetivo de la partiacutecula (Vasir
73
and Labhasetwar 2007) Sin embargo estas estrategias auacuten se encuentran en fase de desarrollo
inicial (Penaloza et al 2017) (Yameen et al 2014)
Se buscoacute un tamantildeo de partiacutecula en la escala submicromeacutetrica (entre 2 y 500 nm) ya que es
necesario para la internalizacioacuten celular y una distribucioacuten raacutepida despueacutes de la administracioacuten
parenteral para alcanzar diferentes tejidos a traveacutes de diferentes barreras bioloacutegicas Las partiacuteculas
de menos de 200 nm minimizan su ingesta por macroacutefagos El tipo de disolvente orgaacutenico la
concentracioacuten del poliacutemero la adicioacuten de surfactante y la energiacutea de emulsioacuten controlan el tamantildeo
del sistema
El meacutetodo O-F68 da lugar a una distribucioacuten monomodal del tamantildeo de partiacuteculas con
diaacutemetros alrededor de 100 nm La adicioacuten de Pluronicreg F68 en la fase orgaacutenica refuerza la
estabilidad coloidal de la primera emulsioacuten y reduce el tamantildeo de partiacutecula en comparacioacuten con
las NP de PLGA en las que la estabilidad es puramente electrostaacutetica debido a los grupos
carboxiacutelicos del PLGA En el meacutetodo W-F68 se tienen en cuenta el esfuerzo cortante y el
volumen de la fase acuosa para producir un sistema con partiacuteculas de entre 100 y 500 nm
Las NP de O-F68-Lys tienen una forma esfeacuterica con una distribucioacuten de tamantildeo monomodal
(diaacutemetros alrededor de 100 nm) y una estructura nuacutecleo-capa (Fig 7a) Las partiacuteculas vaciacuteas
producidas con el meacutetodo O-F68 se muestran en las Figs 7 (sin F68) y Fig 8 (con F68) Tambieacuten
son esfeacutericas y con una estructura nuacutecleo-caparazoacuten pero un poco maacutes grande
W-F68-Lys NP tambieacuten presenta una forma esfeacuterica pero una distribucioacuten de tamantildeo
multimodal con diaacutemetros entre 140 y 450 nm la poblacioacuten maacutes grande estaacute alrededor de 260 nm
(Fig 7b) Tambieacuten se observa una estructura nuacutecleo-capa en estas partiacuteculas Las partiacuteculas vaciacuteas
del meacutetodo W-F68 se presentan en la Fig 9 correspondiente a un sistema maacutes polidisperso
74
(a) (b)
(c)
Figura 7 Morfologiacutea de las nanopartiacuteculas Micrografiacuteas de SEM (a b) y STEM (c) de
nanopartiacuteculas vaciacuteas utilizando el meacutetodo O-F68 sin F68
75
(a) (b)
(c) (d)
Figura 8 Micrografias de SEM (a b) y STEM (c d) de partiacuteculas vaciacuteas usando el meacutetodo O-
F68 con F68
76
(a) (b)
Figura 9 Micrografiacuteas de SEM de partiacuteculas vacias usando el meacutetodo W-F68
11Tamantildeo de las nanopartiacuteculas movilidad electrocineacutetica y estabilidad coloidal
La distribucioacuten del diaacutemetro hidrodinaacutemico de las partiacuteculas se determinoacute en primer lugar por
DLS La Tabla 4 contiene las principales propiedades coloidales de las partiacuteculas producidas con
los meacutetodos O-F68 y W-F68 vaciacuteas o cargadas con lisozima Los resultados de partiacuteculas vaciacuteas
del meacutetodo O-F68 pero sintetizados sin F68 tambieacuten estaacuten incluidos
Los paraacutemetros de tamantildeo se calcularon a traveacutes de un anaacutelisis acumulativo de los datos que
es aplicable para distribuciones de tamantildeo monomodal estrechas (Hassan Rana and Verma
2015) Las micrografiacuteas SEM y STEM indican que se podriacutea suponer tal aproximacioacuten para las
partiacuteculas del meacutetodo O-F68 pero no del W-F68 Por lo tanto las distribuciones de tamantildeo de
intensidad de los diferentes sistemas se muestran en la figura 11a La presencia de Pluronicreg F68
en el meacutetodo O-F68 reduce significativamente el tamantildeo y la polidispersidad de las NP Esto
concuerda con la reduccioacuten de la tensioacuten superficial cuando el F68 estaacute en el interfaz (Tabla 3)
lo que promueve el proceso de emulsioacuten Si las NP tambieacuten estaacuten cargadas con lisozima el tamantildeo
es auacuten menor pero la polidispersidad aumenta ligeramente en comparacioacuten con las partiacuteculas
vaciacuteas Las propiedades surfactantes de la lisozima se han demostrado con los resultados de la
tensioacuten superficial (Tabla 3)
La Fig 11a indica la presencia de partiacuteculas superiores a 500 nm con el W-F68 que no se
correlaciona con las micrografiacuteas SEM Por lo tanto se utilizoacute una teacutecnica diferente (NTA) para
77
obtener informacioacuten sobre la distribucioacuten del tamantildeo de dichos sistemas (figura 12b) Con NTA
la distribucioacuten de tamantildeos fue consistente con las imaacutegenes SEM Se encontraron distribuciones
de gran tamantildeo correspondientes a los sistemas multimodales con este meacutetodo pero la adicioacuten de
lisozima condujo a una clara reduccioacuten del tamantildeo Esto se debe a que la lisozima tambieacuten actuacutea
como emulsionante en la primera emulsioacuten
La carga electrocineacutetica de las NP se analizoacute midiendo la movilidad electroforeacutetica Para
comparacioacuten todas las muestras se midieron a pH 7 (tampoacuten de fosfato) En la Fig 12 se
presentan las distribuciones de movilidad electroforeacutetica mientras que los promedios
correspondientes se muestran en la Tabla 4
Las NP de PLGA normalmente estaacuten cargadas de forma negativa debido a los grupos
carboxiacutelicos del poliacutemero El uso de Pluronicreg F68 en el meacutetodo O-F68 reduce claramente la
movilidad electroforeacutetica de las NP lo que indica que algo de Pluronicreg estaacute ubicado en la
superficie de las NP Esta reduccioacuten se esperaba despueacutes de la incorporacioacuten de este tensioactivo
no ioacutenico al interfaz ya que la presencia de cadenas de oacutexido de polietileno causariacutea un
desplazamiento hacia afuera del plano de corte donde se define el potencial y esto disminuiriacutea
posteriormente la movilidad electroforeacutetica Los resultados previos para partiacuteculas de PLGA han
mostrado una reduccioacuten significativa directamente relacionada con el recubrimiento de
poloxaacutemero (Santander-Ortega et al 2006) Si comparamos los dos sistemas la superficie menos
negativa para las NP OF68 se relacionariacutea con una menor densidad del poliacutemero PLGA de
superficie llevando la carga eleacutectrica negativa a el interfaz Este resultado estariacutea en liacutenea con la
mayor cantidad de PLGA en la formulacioacuten del nanosistema WF68
78
Figura 10 Micrografiacuteas SEM y STEM de partiacuteculas cargadas de lisozima usando el meacutetodo
O-F68 (a) o W-F68 (b)
79
Media Z (nm) PDI Media-μ (micromcmVs)
Meacutetodo
O-F68
Vaciacuteo sin F68 266 plusmn 7 0293 -506 plusmn 015
Vaciacuteo 1627 plusmn 21 0081 -429 plusmn 018
Cargada de
Lisozima 1210 plusmn12 0244 -334 plusmn 007
Meacutetodo
W-F68
Vaciacuteo 273 plusmn 3 0193 -531 plusmn 011
Cargada de
Lisozima 293 plusmn 4 0169 -4212 plusmn 0013
Tabla 4 Propiedades coloidales de PLGA NP de diferentes meacutetodos de formulacioacuten Se
midieron en tampoacuten de fosfato (pH 7) El diaacutemetro hidrodinaacutemico promedio (media Z o media
acumulada) y el iacutendice de polidispersidad (PDI) se determinan a partir de DLS (Media plusmn sd n
= 3)
80
(a)
(b)
Figura 11 Distribucioacuten del diaacutemetro hidrodinaacutemico (a) mediante DLS a pH 7 (tampoacuten de
fosfato) de partiacuteculas de PLGA vaciacuteas y cargadas con lisozima de los meacutetodos O-F68 y W-F68
y (b) mediante NTA a pH 7 (tampoacuten de fosfato) de partiacuteculas de PLGA vaciacuteas y cargadas con
lisozima del meacutetodo W-F68
81
(a)
(b)
Figura 12 Distribucioacuten de movilidad electroforeacutetica a pH 7 (tampoacuten de fosfato) de partiacuteculas
de PLGA cargadas con lisozima y vaciacuteas a partir de los meacutetodos (a) O-F68 y (b) W-F68
82
Cuando la lisozima tambieacuten se utiliza en la siacutentesis la superficie es auacuten menos negativa lo que
podriacutea explicarse por la presencia de alguna proteiacutena (cuya carga neta es positiva) cerca o en el
interfaz Este uacuteltimo efecto tambieacuten se encuentra con el meacutetodo W-F68 La atractiva interaccioacuten
electrostaacutetica entre los residuos de aacutecido terminales negativos de PLGA y las moleacuteculas de
lisozima juega un papel clave en el proceso de encapsulacioacuten de proteiacutenas (Paillard-Giteau et al
2010) o adsorcioacuten (Cai et al 2008) en PLGA NPs que afecta la carga final de proteiacutenas
En relacioacuten con esta situacioacuten una caracteriacutestica importante de la formulacioacuten de
encapsulacioacuten W-F68 es que la fase acuosa tiene un pH de 12 lo que permite una carga neta
negativa de lisozima y por lo tanto evita la atraccioacuten electrostaacutetica de la proteiacutena y el poliacutemero
Esta situacioacuten puede reducir la eficacia de la encapsulacioacuten pero al mismo tiempo favorece el
posterior proceso de difusioacuten de proteiacutenas y en consecuencia la liberacioacuten a corto plazo
Estudios recientes han propuesto el uso de nanopartiacuteculas incrustadas en scaffolds impresos en
3D predisentildeados (Baumann et al 2017) (Lee et al 2017) lo que nos lleva a analizar la
estabilidad de las dos formulaciones en varios medios generalmente empleados durante la
preparacioacuten de otras estructuras Se encontraron distribuciones de tamantildeo similares al original
para las dos formulaciones en diferentes medios (PB PBS y DMEM) y en diferentes momentos
despueacutes de la siacutentesis (0 1 y 5 diacuteas) La carga eleacutectrica de los grupos terminales de aacutecido PLGA
y las moleacuteculas de poloxaacutemero ubicadas en la superficie NP confiere un mecanismo de estabilidad
coloidal combinado electrostaacutetico y esteacuterico como se ha descrito previamente (Manuel J
Santander-Ortega Lozano-Loacutepez et al 2010) (Santander-Ortega et al 2006) Ademaacutes las NPs
en todos los casos mantienen su tamantildeo almacenado a 4 deg C al menos durante 1 mes (datos no
mostrados) Por lo tanto los medios descritos podriacutean usarse potencialmente como medios de
almacenamiento o para preparar otras soluciones o scaffolds antes de colocarlos realmente en el
entorno vivo (in vitro o in vivo)
83
12NMR de las nanopartiacuteculas
En la Fig 12 tanto las NP vaciacuteas como las cargadas de proteiacutenas presentan una movilidad
electroforeacutetica menos negativa que las NP vaciacuteas sin F68 lo que podriacutea explicarse por la presencia
de Pluronicreg F68 en la superficie de la NP Al comparar los espectros 1HNMR de Pluronicreg F68
libre y las NP cargadas con lisozima de los meacutetodos O-F68 y W-F68 podemos verificar la
presencia de F68 en la superficie de las NP (figura 13) por los picos que se muestran entre 325 y
375 ppm y a 1 ppm Estos picos tambieacuten son visibles en los espectros de NP formulados con F68
(O-F68 y W-F68 figuras 14 y 15 respectivamente)
RMN de las nanopartiacuteculas
Figura 13 Espectro 1HMNR de F68 libre
84
Figura 14 Espectro de 1HMNR de partiacuteculas cargadas con lisozima del meacutetodo O-F68
Figura 15 Espectro de 1HMNR de partiacuteculas cargadas con lisozima del meacutetodo W-F68
433 Actividad bioloacutegica e interacciones
F68
F68
F68
F68
85
Una liberacioacuten controlada desde un sistema de administracioacuten basado en PLGA es una tarea
difiacutecil ya que depende de muacuteltiples factores el tipo de PLGA el solvente el estreacutes mecaacutenico el
uso de surfactantes etc (Hines and Kaplan 2013) La difusioacuten de la proteiacutena y la erosioacuten del
poliacutemero son los principales mecanismos implicados en la liberacioacuten de proteiacutena en los sistemas
de administracioacuten basados en PLGA Ademaacutes es tiacutepico encontrar una liberacioacuten en forma de una
raacutepida raacutefaga en la etapa inicial seguida de una fase de liberacioacuten lenta en un corto y mediano
plazo En esta fase las moleacuteculas de proteiacutena se difunden a traveacutes de la matriz del poliacutemero hasta
alcanzar una fase final en la que la degradacioacuten del poliacutemero por hidroacutelisis permite una liberacioacuten
maacutes raacutepida (Fredenberg et al 2011)
Por otro lado la liberacioacuten a corto plazo es de especial intereacutes para el transporte de factores de
crecimiento morfogeneacuteticos oacuteseos (BMP) Una explosioacuten inicial controlada seguida de una
liberacioacuten sostenida mejora significativamente la regeneracioacuten in vivo de hueso (Ortega-Oller et
al 2015) y cartiacutelago (Begam et al 2017) incluso en sistemas de liberacioacuten controlada doble
(Kim and Tabata 2015) Por estas razones centramos nuestro anaacutelisis en la liberacioacuten a corto
plazo teniendo en cuenta la reduccioacuten de la degradacioacuten del poliacutemero por hidroacutelisis encontrada
en sistemas similares para estos primeros pasos (Rescignano et al 2016)
86
Figura 16 Liberacioacuten acumulada (siacutembolos rellenos) y bioactividad residual (siacutembolos
abiertos) de O-F68-Lys (cuadrado) y W-F68-Lys (triaacutengulo) incubados durante diferentes
tiempos a 37 deg C en tampoacuten de fosfato salino (pH 74) (media plusmn sd n = 3)
La Fig 16 muestra la liberacioacuten acumulativa de lisozima de las NP de O-F68-Lys a corto plazo
(siete diacuteas) Estos resultados son consistentes con un proceso de dos pasos un estallido inicial y
uno de liberacioacuten lenta El primer paso podriacutea corresponder a la liberacioacuten de las moleacuteculas de
proteiacutena ubicadas cerca de la superficie cuya presencia se dedujo de los resultados de movilidad
electroforeacutetica (Fig 12) La segunda parte del proceso de liberacioacuten fue limitada y lenta debido a
la difusioacuten de proteiacutenas a traveacutes de la matriz de la cubierta polimeacuterica La interaccioacuten electrostaacutetica
especiacutefica entre las moleacuteculas de lisozima positiva y los grupos dxe aacutecido terminal negativo de
PLGA puede reducir la difusioacuten de proteiacutenas (Blanco and Alonso 1998) Cuando se agrega el
poloxaacutemero (F68) la interaccioacuten entre el surfactante y la proteiacutena ayuda al proceso de difusioacuten
lo que lleva a una liberacioacuten maacutes completa y sostenida (Manuel J Santander-Ortega Csaba et
87
al 2010) Tambieacuten ayuda a mantener la actividad bioloacutegica de la proteiacutena (Paillard-Giteau et al
2010) (Morille et al 2013) El poloxaacutemero reduce las interacciones proteiacutena-poliacutemero no
especiacuteficas (es decir interacciones hidroacutefobas) pero no las especiacuteficas (electrostaacutetica) por lo
tanto la difusioacuten a traveacutes de los poros llenos de agua o a traveacutes del poliacutemero sigue siendo limitada
En el estudio actual la fraccioacuten de proteiacutena liberada y el patroacuten de liberacioacuten son similares a los
encontrados en la literatura para la lisozima encapsulada en nano y micropartiacuteculas de mezclas de
PLGA y otros poliacutemeros o surfactantes (Meng et al 2003) (White et al 2013) (Perez De Jesus
and Griebenow 2002)
La curva de liberacioacuten de proteiacutenas de W-F68-Lys NP (Fig 16) revela que la tasa de
administracioacuten inicial es ideacutentica a la del sistema O-F68 lo que podriacutea significar una proporcioacuten
similar de proteiacutena encapsulada cerca o en la superficie para ambos sistemas NP Esto estariacutea de
acuerdo con la disminucioacuten anaacuteloga en la movilidad electroforeacutetica de las NP cargadas con
lisozima de la que informaron previamente (Fig 12) En la segunda parte del proceso la
interaccioacuten especiacutefica entre la proteiacutena y el poliacutemero estaacute nuevamente presente Sin embargo el
proceso de difusioacuten en el sistema W-F68 parece mejorar permitiendo una liberacioacuten continua y
sostenida despueacutes del estallido inicial y alcanzando un valor ligeramente maacutes alto para el tiempo
de liberacioacuten maacuteximo estudiado Este resultado podriacutea estar relacionado con la estructura interna
de la capa de poliacutemero que permite una mejor hidratacioacuten y por lo tanto una mejor difusioacuten de
la proteiacutena hacia el exterior Se ha informado previamente que el uso de disolventes orgaacutenicos
menos polares tales como DCM para formulaciones de partiacuteculas de PLGA aumenta la densidad
de la matriz de poliacutemero en comparacioacuten con disolventes orgaacutenicos maacutes polares tales como EA
Las matrices PLGA resultan maacutes resistentes en el primer caso pero reducen al mismo tiempo su
conectividad y difusioacuten (Bohr et al 2015) Meng y colaboradores (Meng et al 2003) encontraron
que una eliminacioacuten maacutes raacutepida de EA da como resultado una liberacioacuten cineacutetica maacutes lenta de la
proteiacutena debido a una disminucioacuten en la porosidad de las NP En cuanto al papel de Pluronicreg
Rafati y colaboradores (Rafati et al 2012) encontraron una mayor concentracioacuten de proteiacutena
88
encapsulada en los poros superficiales en micropartiacuteculas sintetizadas en presencia de surfactante
en la segunda fase acuosa de la emulsioacuten Dado que se introdujo un paso intermedio en nuestra
formulacioacuten W-F68 en la segunda fase acuosa de la emulsioacuten la eliminacioacuten de la EA por difusioacuten
se controloacute fuertemente de modo que se esperaba que la porosidad de estas NP aumentara Esta
porosidad mejora la difusioacuten de proteiacutenas lo que permite un patroacuten de liberacioacuten maacutes estable de
acuerdo con el resultado experimental encontrado para este sistema A pesar del efecto
desfavorable de la interaccioacuten proteiacutena-poliacutemero electrostaacutetico especiacutefico en la liberacioacuten la
cantidad de proteiacutena liberada en nuestras NP es sustancial lo que significa que hay otras
interacciones inespeciacuteficas que pueden ser moduladas por la presencia de surfactante permitiendo
un lanzamiento sostenido La cantidad de lisozima liberada es similar a la encontrada con la
lisozima fiacutesicamente adsorbida en la superficie de las nanopartiacuteculas de PLGA a pesar de la
atraccioacuten electrostaacutetica (Cai et al 2008) Ademaacutes de otras interacciones inespeciacuteficas la
concentracioacuten de electrolitos en el medio de liberacioacuten podriacutea modular esta atraccioacuten
electrostaacutetica entre la proteiacutena y el poliacutemero disminuyeacutendola y facilitando el proceso de
liberacioacuten (Manuel J Santander-Ortega Lozano-Loacutepez et al 2010)
Otro paraacutemetro notable es la actividad bioloacutegica de la liberacioacuten in vitro de lisozima que se
muestra en la Fig 16 Mientras que en el sistema O-F68 la bioactividad se reduce parcialmente
hasta en un 40 la proteiacutena suministrada por el sistema W-F68 mantiene la actividad por encima
del 90 con respecto a la de la lisozima suministrada comercialmente y se resuspende en el mismo
tampoacuten de liberacioacuten Como se discutioacute anteriormente tanto el solvente orgaacutenico como la
interaccioacuten hidrofoacutebica entre la proteiacutena y el poliacutemero a menudo causan la desnaturalizacioacuten de
las proteiacutenas encapsuladas (Paillard-Giteau et al 2010) (Gaudana et al 2013) Perez y
colaboradores (Perez De Jesus and Griebenow 2002) describen una peacuterdida parcial de actividad
cuando se usa DCM y una solucioacuten acuosa de PVA en la segunda etapa de emulsioacuten sin ninguacuten
excipiente adicional El uso de poloxaacutemeros en la formulacioacuten reduce tales interacciones mejora
la estabilidad de la proteiacutena y mantiene una capa acuosa que retiene las moleacuteculas de agua
89
necesarias para la funcioacuten bioloacutegica de la proteiacutena al mismo tiempo que ayuda a su difusioacuten Esta
situacioacuten junto con el uso de un solvente orgaacutenico deacutebil como EA ayuda a preservar la actividad
bioloacutegica de la lisozima como se encuentra para el sistema W-F68-Lys
La Fig 17 presenta diferentes imaacutegenes de microscopiacutea confocal relacionadas con el proceso
de liberacioacuten de NPs W-F68 cargadas de lisozima Una disminucioacuten en la intensidad de
fluorescencia fue apreciable a lo largo del experimento in vitro Ademaacutes la agregacioacuten del sistema
es visible a medida que avanza el proceso de incubacioacuten El anaacutelisis de estas imaacutegenes es
consistente con los resultados informados previamente para este sistema NP
90
Figura 17 Actividad bioloacutegica e interacciones Imaacutegenes octogonales obtenidas por
microscopiacutea confocal de Lisozima-FITC encapsulado en NPs W-F68 tras su incubacioacuten a
diferentes tiempos en tampon fosfato salino (pH 74) a 37ordmC Las NP se centrifugaron previamente
para eliminar la proteiacutena marcada liberada (a) Experimentos previos al lanzamiento (b)
despueacutes de 24h (c) despueacutes de 72h (d) despueacutes de 168h
13Captacioacuten celular
La captacioacuten celular de NP de PLGA es un proceso conocido que se ve afectado principalmente
por las propiedades de superficie la funcionalizacioacuten (Loureiro et al 2016) y la agregacioacuten de
partiacuteculas (Xiong et al 2011) La internalizacioacuten y el procesamiento intracelular posterior de las
partiacuteculas se ha descrito como un proceso activo por lo tanto depende de la energiacutea y puede
verse afectado por otros factores que alteran la absorcioacuten de energiacutea por parte de las ceacutelulas como
la temperatura (Penaloza et al 2017) Las partiacuteculas pueden internalizarse mediante varios
meacutetodos de endocitosis que dependen principalmente del tamantildeo de la partiacutecula partiacuteculas
(a) (b)
(c) (d)
91
dependientes de caveolina (diaacutemetro asymp 60 nm) independientes de clatrina (diaacutemetro asymp 90 nm) y
dependientes de clatrina (diaacutemetro asymp 120 nm) (Vasir and Labhasetwar 2007) (Yameen et al
2014) Una vez internalizados alrededor del 65 se exportan al espacio extracelular antes de
liberar cualquiera de sus contenidos mientras que el resto libera lentamente la moleacutecula
encapsulada en el espacio intracelular (Panyam and Labhasetwar 2003)
Figura 18 Proyeccioacuten z de 5 imaacutegenes de hMSCs visualizadas 30 minutos despueacutes de la
incubacioacuten con W-F68-LysFITCNPsorO-F68-LysFITCNPs Las hMSC se marcaron previamente
con Guiacutea celular roja Barra de escala 20 m
El proceso de liberacioacuten intracelular se ve afectado por la formulacioacuten de las partiacuteculas
(Penaloza et al 2017) Hemos demostrado que los sistemas propuestos siguen un patroacuten similar
a otros publicados anteriormente En un periodo de tiempo tan corto como 30 minutos despueacutes
de la incubacioacuten las NPs de W-F68-LysFITC fueron absorbidas por las ceacutelulas (Fig 18) Algunas
partiacuteculas W-F68 todaviacutea estaban en el medio por lo que la actividad dual podriacutea ocurrir Por el
92
contrario las NP de O-F68-LysFITC se vieron afectadas por la agregacioacuten y por lo tanto no
alcanzaron adecuadamente el espacio intracelular (Fig 18 para las imaacutegenes del eje Z veacutease la
Fig 19)
Superposicioacuten Canal Verde Canal Rojo P
royec
cioacuten z
93
Superposicioacuten Canal Verde Canal Rojo
Pro
yec
cioacuten z
94
Figura 19 Proyeccioacuten z de 5 imaacutegenes e imaacutegenes z independientes de hMSC visualizadas 30
minutos despueacutes de la incubacioacuten sin partiacuteculas y con NP W-F68-LysFITC o NP O-F68-LysFITC
Las hMSC se marcaron previamente con un rastreador celular rojo Barra de escala 20 microm
Pro
yec
cioacuten z
Superposicioacuten Canal Verde Canal Rojo
95
Esto contradice los anaacutelisis previos de la estabilidad coloidal en PB PBS y DMEM Este
hallazgo puede explicarse por el hecho de que aunque el medio de cultivo fue DMEM este uacuteltimo
medio se complementoacute con suero fetal bovino y las ceacutelulas liberan muchos factores al medio
extracelular que pueden afectar a este tipo de partiacuteculas Ninguno de los sistemas demostroacute ser
toacutexico para las ceacutelulas (Fig 20) No hay estudios disponibles que hayan informado alguacuten efecto
de la lisozima sobre las hMSC
Figura 20 Pruebas de viabilidad a las 6 horas de antildeadir diferentes dosis de partiacuteculas A
continuacioacuten se separaron las ceacutelulas se tintildeeron con azul tripaacuten y se contaron las ceacutelulas vivas
o muertas El graacutefico representa ceacutelulas vivas normalizadas y la desviacioacuten estaacutendar de tres
experimentos No se encuentran diferencias
0
02
04
06
08
1
12
14
16
Control W-F68
10microlml
W-F68
5microlml
O-F68
10microlml
O-F68 5microlml
Viability after 6 hours
Viabilidad 6 horas despueacutes
96
5FORMULACIOacuteN CARACTERIZACIOacuteN COLOIDAL Y EFECTO
BIOLOacuteGICO IN VITRO DE NANOPARTIacuteCULAS DE PLGA
CARGADAS CON BMP-2 PARA LA REGENERACIOacuteN OacuteSEA
51 Antecedentes
En el contexto de la nanomedicina la regeneracioacuten de tejidos usando micro y nano estructuras
coloidales que tienen un tamantildeo y actividad superficial uacutenicos ha recibido una atencioacuten creciente
en los uacuteltimos antildeos Se han realizado muchos esfuerzos para mejorar la ingenieriacutea de estos nano-
sistemas con el fin de alcanzar una entrega inteligente de moleacuteculas bioactivas para optimizar
sus ventajas terapeacuteuticas y minimizar los efectos secundarios nocivos (van Rijt and Habibovic
2017) Con este objetivo hay descrito un amplio espectro de nanoportadores biocompatibles que
muestran propiedades adecuadas para diferentes aplicaciones bioloacutegicas y terapeacuteuticas (Kumar et
al 2017) Entre estas variadas propuestas los nanosistemas polimeacutericos representan un grupo
importante siendo el PLGA uno de los maacutes utilizados debido a las propiedades comentadas
anteriormente a lo largo de este trabajo entre ellas destaca su biocompatibilidad
biodegradabilidad y baja citotoxicidad asi como su capacidad para administrar una amplia
variedad de moleacuteculas y faacutermacos activos moleacuteculas sinteacuteticas o naturales con propiedades
hidrofiacutelicas o hidrofoacutebicas y biomoleacuteculas de proteiacutenas a aacutecidos nucleicos (Danhier et al 2012)
(Ding and Zhu 2018) (Arias et al 2015) obteniendo la aprobacioacuten de diferentes agencias
farmaceacuteuticas para uso humano (Mir Ahmed and Rehman 2017) (Jana and Jana 2017)
Dentro de la ingenieriacutea de tejidos en el campo de la odontologiacutea las teacutecnicas de regeneracioacuten
oacutesea empiezan a despertar un gran intereacutes por parte de los diferentes profesionales de la salud
contribuyendo con ello a un mayor desarrollo de estas liacuteneas de investigacioacuten y generando una
mayor tendencia a diferentes viacuteas de crecimiento relacionadas con el subsanamiento de los
obstaacuteculos que pueden originarse durante el proceso de encapsulacioacuten de moleacuteculas hidrofilicas
o en el de funcionalizacioacuten especiacutefica de la superficie a fin de mejorar la versatilidad de las
diferentes moleculas supuestos eacutestos en los que el PLGA es el poliacutemero de referencia para la
97
comunidad cientiacutefica en aras de crear NP para favorecer la cicatrizacioacuten oacutesea (Bapat et al 2019)
La literatura describe el suministro de moleacuteculas bioactivas normalmente factores de crecimiento
utilizando micropartiacuteculas polimeacutericas (MP) y NP con PLGA como componente principal
(Ortega-Oller et al 2015) Entre los factores de crecimiento morfogeneacutetico oacuteseo la BMP-2
(proteiacutena morfogeneacutetica oacutesea 2) ha sido la maacutes citada con muchos ejemplos en los que la
encapsulacioacuten o la adsorcioacuten en la superficie permite una eficiencia de atrapamiento adecuada y
diversos patrones de liberacioacuten (Ji et al 2010) (Kirby et al 2011) (Qutachi Shakesheff and
Buttery 2013) (Wang et al 2015) (Zhang et al 2016) Para las proteiacutenas con una vida media
muy corta como las BMP los nanosistemas PLGA biodegradables proporcionan proteccioacuten y
una dosis oacuteptima para una estimulacioacuten adecuada de la diferenciacioacuten celular (Begam et al 2017)
(Balmayor et al 2009)
Por lo tanto dentro de este escenario en el presente trabajo buscamos optimizar un sistema
de nanopartiacuteculas para llevar a cabo y controlar la liberacioacuten de BMP-2 utilizando como punto de
partida el procedimiento de siacutentesis de un sistema de NP cargado de lisozima previamente
descrito para la encapsulacioacuten de ese modelo de proteiacutena (Ortega-Oller et al 2017) Ademaacutes
para encapsular BMP-2 preparamos un segundo sistema en el que esta proteiacutena se co-adsorbioacute
con albuacutemina de suero bovino en la superficie de NP vaciacuteas Hemos estudiado el tamantildeo y la
morfologiacutea la eficiencia de la encapsulacioacuten de proteiacutenas las caracteriacutesticas de la superficie y la
estabilidad coloidal y temporal para completar la caracterizacioacuten fisicoquiacutemica de ambos sistemas
NP
El perfil de liberacioacuten de BMP-2 indica el potencial de un nanoportador PLGA para la
regeneracioacuten oacutesea y depende en gran medida de la degradacioacuten del poliacutemero por hidroacutelisis (Xu et
al 2017) Sin embargo a corto plazo durante el cual la liberacioacuten no depende de esta degradacioacuten
quiacutemica es necesario un control adecuado de la liberacioacuten para modular otros procesos fiacutesicos
Por lo tanto enfocamos nuestros experimentos de liberacioacuten a corto plazo utilizando diferentes
teacutecnicas para comparar las dos muestras de NP y establecer los perfiles de liberacioacuten de BMP-2
98
correspondientes Finalmente la actividad bioloacutegica (migracioacuten celular proliferacioacuten y
diferenciacioacuten osteogeacutenica) se proboacute in vitro utilizando ceacutelulas estromales mesenquimales (MSC)
derivadas del hueso alveolar (Padial-Molina et al 2019)
52 Materiales y Meacutetodos
521Siacutentesis de nanoparticulas
14Formulacioacuten
El aacutecido poli (lactico-co-glicolico) (PLGA 5050) ([C2 H2 O2]x [C3 H4 O2]y) x = 50 y = 50
(Resomerreg 503H) 32 - 44 kDa se usoacute como poliacutemero El surfactante polimeacuterico Pluronicreg F68
(Poloxaacutemero 188) (Sigma-Aldrich) se usoacute como el emulsionante Su estructura se basa en un
copoliacutemero tri-bloque de poli (oacutexido de etileno) PEO y poli (oacutexido de propileno) PPO (PEO)a ndash
(PPO)b ndash (PEO)a con a = 75 y b = 30 La lisozima de huevo de gallina (Sigma-L7651) se usoacute
como proteiacutena hidroacutefila La proteiacutena morfogeneacutetica oacutesea recombinante humana rhBMP-2
(Sigma-H4791) se utilizoacute como biomoleacutecula terapeacuteutica El agua se purificoacute en un sistema Milli-
Q Academic Millipore Se usoacute un meacutetodo de siacutentesis de doble emulsioacuten siguiendo un
procedimiento previamente descrito con ligeras modificaciones (Ortega-Oller et al 2017) En
este meacutetodo se disolvieron 100 mg de PLGA y 3 mg de aacutecido desoxicoacutelico (DC) en un tubo que
conteniacutea 1 ml de acetato de etilo (EA) y se sometieron a voacutertice En total se agregaron 40 μL de
una solucioacuten tamponada a pH 128 con o sin rhBMP-2 (200 μg mL) y se sonicoacute de inmediato
(Branson Ultrasonics 450 Analifier Sonifier) durante 1 min (Dial del ciclo de trabajo 20 Dial
de control de salida 4) con el tubo rodeado de hielo Esta emulsioacuten primaria de WO se vertioacute en
un tubo de plaacutestico que conteniacutea 2 ml de una solucioacuten tamponada (pH 12) de F68 a 1 mg ml y
se agitoacute en voacutertex durante 30 s Luego el tubo rodeado de hielo se sonicoacute a la amplitud maacutexima
de la micro punta durante 1 minuto (control de salida 7) Esta segunda emulsioacuten WOW se vertioacute
en un vaso que conteniacutea 10 ml de la solucioacuten F68 tamponada y se mantuvo bajo agitacioacuten
magneacutetica durante 2 minutos El disolvente orgaacutenico se extrajo raacutepidamente por evaporacioacuten al
99
vaciacuteo hasta un volumen final de 8 ml Los sistemas de NP encapsulados vaciacuteos y BMP-2
resultantes se denominaron NP y NP-BMP2 respectivamente En la Figura 21 se muestra un
esquema detallado del procedimiento de siacutentesis con un rendimiento basado en el componente
PLGA siempre superior al 85
15Limpieza y almacenamiento
Despueacutes de la evaporacioacuten del solvente orgaacutenico la muestra se centrifugoacute durante 10 minutos
a 20 deg C a 12000 rpm El sobrenadante se filtroacute usando nanofiltros Millipore 01 μm para medir
la proteiacutena libre no encapsulada El sedimento se resuspendioacute en tampoacuten fosfato (NaH2PO4 115
mM) PB hasta un volumen final de 4 ml y se mantuvo refrigerado a 4ordmC En estas condiciones
los sistemas mantuvieron la estabilidad coloidal al menos durante un mes
16Carga de proteiacutenas y eficiencia de encapsulacioacuten
La carga inicial de proteiacutenas se optimizoacute para la formulacioacuten de nanopartiacuteculas preservando
la estabilidad coloidal final despueacutes de la etapa de evaporacioacuten y teniendo en cuenta las cantidades
mostradas en la literatura para este factor de crecimiento cuando se encapsula dentro de NPs de
PLGA (drsquoAngelo et al 2010) (Chang et al 2017) Por lo tanto elegimos 2 μg como la masa total
inicial de rhBMP-2 lo que significa una relacioacuten de 2 10 5 p p (rhBMP-2 PLGA) La
cantidad de rhBMP-2 encapsulado se calculoacute midiendo la diferencia entre la cantidad agregada
inicial y la proteiacutena libre no encapsulada presente en el sobrenadante despueacutes de la etapa de
limpieza que se proboacute mediante un ensayo inmuno-absorbente ligado a enzimas especiacuteficas
siguiendo las instrucciones del fabricante (ELISA kit RAB0028 de Sigma-Aldrich St Louis
MO EE UU) Luego la eficiencia de encapsulacioacuten de proteiacutenas (EE) se calculoacute de la siguiente
manera
EE = 119872119868minus119872119865
119872119868119909 100
donde MI es la masa total inicial de rhBMP-2 y MF es la masa total de rhBMP-2 en el
sobrenadante acuoso
100
17Adsorcioacuten Fiacutesica de Proteiacutenas
La albuacutemina de suero bovino (BSA) y la rhBMP-2 se acoplaron en la superficie de
nanopartiacuteculas vaciacuteas mediante un meacutetodo de adsorcioacuten fiacutesica El volumen apropiado de una
solucioacuten de proteiacutena acuosa que conteniacutea 05 mg de BSA y 2 μg de rhBMP-2 se mezcloacute con 5 ml
de tampoacuten de acetato (pH 5) que conteniacutea NP vaciacuteas con 125 mg de PLGA Esto proporcionoacute
una cantidad inicial de proteiacutenas correspondiente al 004 p p (proteiacutena PLGA) mientras que
la relacioacuten de masa entre proteiacutenas fue de 04 p p (rhBMP-2 BSA) Esta solucioacuten se incuboacute a
temperatura ambiente durante 2 h con agitacioacuten mecaacutenica Las nanopartiacuteculas se separaron de la
solucioacuten de tampoacuten por centrifugacioacuten y despueacutes de que se filtraran los sobrenadantes
(nanofiltros Millipore 01 μm) se analizaron cualitativamente por electroforesis en gel mientras
que la cuantificacioacuten de proteiacutenas se realizoacute mediante un ensayo de proteiacutena de aacutecido
bicinconiacutenico (BCA) (Sigma-Aldrich St Louis MO EE UU) Para BSA y el ELISA especiacutefico
para rhBMP-2 El sedimento de nanopartiacuteculas se resuspendioacute en tampoacuten fosfato (pH 74) y se
almacenoacute a 4ordmC Este sistema se denominoacute NP-BSA-BMP2
18Separacioacuten de proteiacutenas por electroforesis en gel SDS-PAGE
Las NP cargadas de proteiacutena y los diferentes sobrenadantes se trataron a 90 deg C durante 10
minutos en el siguiente tampoacuten Tris-HCl 625 mM (pH 68 a 25 deg C) dodecil sulfato de sodio al
2 (p v) (SDS) 10 de glicerol 001 (p v) de azul de bromofenol ditiotreitol (DTT) 40
mM Las muestras se separaron luego por tamantildeo en gel de poliacrilamida poroso al 12
(electroforesis en gel de poliacrilamida SDS 1D) bajo el efecto de un campo eleacutectrico La
electroforesis se realizoacute a voltaje constante (130 V 45 min) y los geles se tintildeeron usando una
solucioacuten de azul de Coomassie (01 de azul brillante de Coomassie R-250 50 de metanol y
10 de aacutecido aceacutetico glacial) y se destintildeeron con la misma solucioacuten que carece del tinte
101
522 Caracterizacioacuten de nanopartiacuteculas morfologiacutea tamantildeo concentracioacuten y movilidad
electrocineacutetica
Se obtuvieron imaacutegenes de NP mediante microscopiacutea electroacutenica de barrido (SEM) con un
microscopio electroacutenico de barrido de emisioacuten de campo SUPRA 40VP Zeiss del Centro de
Instrumentacioacuten Cientiacutefica de la Universidad de Granada (CIC UGR)
La distribucioacuten del tamantildeo hidrodinaacutemico de las NP se evaluoacute mediante anaacutelisis de
seguimiento de nanopartiacuteculas (NTA) con un NanoSight LM10-HS (GB) FT14 (NanoSight
Amesbury Reino Unido) y una caacutemara sCMOS La concentracioacuten de partiacuteculas de acuerdo con
el diaacutemetro (distribucioacuten de tamantildeos) se calculoacute como un promedio de al menos tres
distribuciones de tamantildeos independientes La concentracioacuten total de NP de cada sistema se
determinoacute para controlar el nuacutemero de partiacuteculas utilizadas en los experimentos celulares Las
condiciones de medicioacuten para todas las muestras fueron 25 deg C una viscosidad de 089 cP un
tiempo de medicioacuten de 60 s y una ganancia de caacutemara de 250 El obturador de la caacutemara fue de
11 y 15 ms para las NP vaciacuteas y cargadas con BMP respectivamente El umbral de deteccioacuten se
fijoacute en 5
La movilidad electroforeacutetica de las NP se determinoacute utilizando un dispositivo Zetasizerreg
NanoZeta ZS (Malvern Instrument Ltd Malvern Reino Unido) que funciona a 25 deg C con un
laacuteser He-Ne de 633 nm y un aacutengulo de dispersioacuten 173 Cada punto de datos se tomoacute como un
promedio sobre tres mediciones de muestra independientes Para cada muestra la distribucioacuten de
movilidad electroforeacutetica y la movilidad electroforeacutetica promedio (μ-promedio) se determinaron
mediante la teacutecnica de electroforesis Doppler laacuteser
523 Estabilidad coloidal y temporal en medios bioloacutegicos
El diaacutemetro hidrodinaacutemico promedio y el iacutendice de polidispersidad (PDI) por dispersioacuten
dinaacutemica de la luz (DLS) de cada sistema de NP se midieron en diferentes medios (tampoacuten de
fosfato (PB) tampoacuten de fosfato salino (PBS) y medio de cultivo celular Medio de Eagle
modificado de Dulbecco DMEM (Sigma)) Ademaacutes los datos sobre la estabilidad temporal se
102
obtuvieron repitiendo estos anaacutelisis en diferentes momentos despueacutes de la siacutentesis (0 1 y 5 diacuteas)
y despueacutes de 1 mes en condiciones de almacenamiento
Los experimentos de liberacioacuten in vitro se realizaron de la siguiente manera 1 ml de cada
muestra para cada tiempo de incubacioacuten se suspendioacute en PBS a 37 deg C Despueacutes del tiempo
correspondiente (24 48 96 168 h) las NP se separaron del sobrenadante de las proteiacutenas
liberadas por centrifugacioacuten durante 10 min a 14000 rpm (10 C) El sedimento de NP se suspendioacute
en 1 ml de NaOH 005 M y se agitoacute durante 2 h para una degradacioacuten completa del poliacutemero La
solucioacuten de proteiacutena alcalina se analizoacute mediante BCA y ELISA para cuantificar la cantidad
ineacutedita La proteiacutena liberada se calculoacute teniendo en cuenta la cantidad encapsulada total Todos
los experimentos se realizaron por triplicado
524 Interacciones celulares
Para todos los estudios bioloacutegicos in vitro se utilizoacute una poblacioacuten celular cultivada del hueso
alveolar maxilar Esta poblacioacuten se caracterizoacute previamente y se confirmoacute que presentaba todas
las caracteriacutesticas de una poblacioacuten de ceacutelulas del estroma mesenquimatoso (MSC) (Padial-
Molina et al 2019) Las ceacutelulas se tomaron de donantes humanos sanos despueacutes de la aprobacioacuten
del Comiteacute de Eacutetica para la Investigacioacuten Humana de la Universidad de Granada (424 CEIH
2018) Medio de Eagle modificado por Dulbecco regular (DMEM) con 1 g L de glucosa
(DMEM-LG) (Gibco) suero bovino fetal al 10 (FBS) (Sigma-Aldrich St Louis MO EE UU)
1 100 de aminoaacutecidos no esenciales (NEAA) (Gibco) 001 μg ml de factor de crecimiento de
fibroblastos baacutesico (bFGF) (PeproTech Londres Reino Unido) 100 U ml de penicilina
estreptomicina y 025 μg ml de anfotericina B utilizado como medio de cultivo para todos los
experimentos Los cultivos se mantuvieron a 37 deg C en una atmoacutesfera de CO2 al 5 (2000 ceacutelulas
pocillo) Todos los experimentos bioloacutegicos se repitieron por triplicado al menos 3 veces por
condicioacuten
19Migracioacuten Celular
103
Un ensayo de migracioacuten celular se realizoacute como se describioacute anteriormente (Padial-Molina
Volk and Rios 2014) (Liang Park and Guan 2007) Brevemente las MSC se distribuyeron en
tres pocillos para cada condicioacuten y se les permitioacute crecer hasta una confluencia celular cercana al
99 en 24 pocillos placa de 3000 ceacutelulas cm2 y en cada pocillo se realizaron tres rasguntildeos
diferentes Luego las ceacutelulas se privaron de hambre durante 24 h mediante la adicioacuten de medio
de cultivo sin suero Se hizo un rasguntildeo usando una punta de pipeta a lo largo del diaacutemetro del
pozo Se realizoacute un paso de lavado con PBS para eliminar las ceacutelulas rayadas Se antildeadieron nuevos
medios de cultivo completos y se suplementaron seguacuten el grupo asignado (BMP-2 NP-BMP2 y
NP-BSA-BMP2 a 125 25 y 5 ng ml de BMP-2) Posteriormente se tomaron nueve imaacutegenes
de la misma aacuterea en cada condicioacuten hasta 48 h maacutes tarde En estas imaacutegenes el aacuterea raspada se
midioacute con el software ImageJ (Instituto Nacional de Salud Bethesda MD EUA
(httprsbwebnihgovij) La reduccioacuten en el aacuterea rayada con el tiempo se midioacute considerando el
aacuterea en el tiempo 0 como 100 abierta
20Proliferacioacuten celular
La proliferacioacuten se evaluoacute mediante un ensayo de sulforhodamina (SRB) (Houghton et al
2007) El ensayo se realizoacute sembrando las ceacutelulas a 1500 ceacutelulas cm2 en una placa de 96 pocillos
a una confluencia no superior al 50 Despueacutes de la unioacuten celular se agregaron los diferentes
suplementos (BMP-2 NP-BMP2 y NP-BSA-BMP2 a 125 25 y 5 ng ml de BMP-2) y las ceacutelulas
se mantuvieron en cultivo durante 7 diacuteas En cada punto de tiempo las ceacutelulas se lavaron con PBS
1X y se fijaron antildeadiendo aacutecido tricloroaceacutetico al 10 enfriado con hielo durante 20 minutos a
4ordmC Luego las ceacutelulas se lavaron 3 veces con dH2O y se secaron hasta que se recogieron todos
los puntos de tiempo Cada pocillo recibioacute 04 de SRB en 1 de aacutecido aceacutetico durante 20
minutos a temperatura ambiente con agitacioacuten suave La tincioacuten se terminoacute lavando cada pocillo
3 veces con aacutecido aceacutetico al 1 y secaacutendolo a temperatura ambiente durante 24 h El colorante se
recuperoacute de las ceacutelulas antildeadiendo Tris Base 10 mM a pH 105 y agitando suavemente durante 10
104
minutos La solucioacuten recuperada se distribuyoacute luego en una placa de 96 pocillos y se leyoacute la
absorbancia oacuteptica a 492 nm
bullDiferenciacioacuten osteogeacutenica
La diferenciacioacuten osteogeacutenica se evaluoacute mediante la adicioacuten de medios osteogeacutenicos al cultivo
celular en combinacioacuten con BMP-2 NP-BMP2 y NP-BSA-BMP2 libres a las dosis maacutes altas
utilizadas en experimentos anteriores Las ceacutelulas se sembraron a 3000 ceacutelulas cm2 y se
cultivaron para alcanzar una confluencia del 85 al 90 Esto fue seguido por la adicioacuten de
medios de induccioacuten que conteniacutean 10 mM de glicerofosfato (Fluka 50020) 01 μM de
dexametasona (Sigma-Aldrich D2915) y 005 mM de aacutecido L-ascoacuterbico (Sigma-Aldrich
A8960) Los cultivos celulares se mantuvieron durante 7 diacuteas para analizar la actividad temprana
En el diacutea 7 las ceacutelulas se recogieron en 1 ml de TRIzolreg Luego se extrajo el ARN y se convirtioacute
en ADNc Luego se evaluoacute la fosfatasa alcalina (ALP) y se calculoacute la expresioacuten con respecto a
la proteiacutena gliceraldehiacutedo-3-fosfato deshidrogenasa (GAPDH) por el meacutetodo 2 DDCt Estos
procedimientos se llevaron a cabo como describe en otra parte el siguiente autor (Padial-Molina
et al 2019) Las secuencias de cebador directo e inverso fueron
AGCTCATTTCCTGGTATGACAAC y TTACTCCTTGGAGGCCATGTG para GAPDH
TCCAGGGATAAAGCAGGTCTTG y CTTTCTCTTTCTCTGGCACTAAGG para ALP
bullEvaluacioacuten estadiacutestica
La migracioacuten y la proliferacioacuten celular se evaluaron mediante ANOVA seguido de la prueba
de comparaciones muacuteltiples de Tukey para el anaacutelisis por pares La comparacioacuten entre los niveles
de ALP a los 4 frente a los 7 diacuteas se analizoacute mediante un par de pruebas t de Student En todos los
casos se establecioacute un valor p inferior a 005 como significacioacuten estadiacutestica
105
53 Resultados y Discusioacuten
531Formulacioacuten de nanoparticulas
La evaporacioacuten de doble emulsioacuten-solvente ha sido descrita como un meacutetodo robusto y de uso
frecuente para producir NP de PLGA cargadas con biomoleacuteculas (Ding and Zhu 2018)
(McClements 2018) (Ortega-Oller et al 2015) (Iqbal et al 2015) Una formulacioacuten previamente
optimizada por nuestro grupo permitioacute la preservacioacuten de la actividad bioloacutegica de biomoleacuteculas
encapsuladas usando un solvente orgaacutenico ligeramente agresivo Ademaacutes el aacutecido desoxicoacutelico
se ha utilizado en el primer paso de la formulacioacuten para mejorar la estabilidad coloidal de las NP
y simultaacuteneamente para obtener superficies de NP enriquecidas con grupos carboxiacutelicos
mejorando su versatilidad y permitiendo que un quiacutemico posterior de lugar a la inmovilizacioacuten de
diferentes ligandos especiacuteficos (Sanchez-Moreno et al 2013) Por medio de esta formulacioacuten
mejorada en el presente trabajo desarrollamos nanopartiacuteculas vaciacuteas (NP) o nanopartiacuteculas que
encapsulan rhBMP-2 (NP-BMP2)
106
Una descripcioacuten esquemaacutetica del procedimiento de siacutentesis se muestra en la Figura 21
Figura 21 Esquema de la formulacioacuten de NP-BMP2
Para NP-BMP2 logramos una eficiencia de encapsulacioacuten de proteiacutenas (EE) de 97 plusmn 2 Este
resultado es estable en la literatura en la que varios autores han reportado valores igualmente altos
que encapsulan esta proteiacutena dentro de nanopartiacuteculas y micropartiacuteculas de PLGA (Lochmann et
al 2010) (Kempen et al 2008) Nuestra formulacioacuten tiene varios factores que conducen a este
elevado valor de EE La baja relacioacuten proteiacutena poliacutemero en masa (Manuel J Santander-Ortega
Csaba et al 2010) la afinidad de rhBMP-2 a una interaccioacuten inespeciacutefica con superficies
hidrofoacutebicas (Lochmann et al 2010) o la adicioacuten de estabilizadores (poloxaacutemero) en el segundo
paso del procedimiento de doble emulsioacuten (Ortega-Oller et al 2015) La ausencia de rhBMP-2
en el sobrenadante resultante de la etapa de centrifugacioacuten en el proceso de limpieza se verificoacute
mediante ELISA y SDS-PAGE en el que se muestra una banda clara correspondiente a 14 kD de
cadenas polipeptiacutedicas rhBMP-2 para el carril A en la Figura 22 correspondiente a NP-BMP2
La masa de proteiacutena encapsulada alrededor de 2 microg es similar a la de diferentes micro y
107
nanosistemas PLGA descritos en la literatura (Wang et al 2015) (Chung et al 2007) (La et al
2010) Teniendo en cuenta las condiciones de almacenamiento para nuestras muestras esto
corresponde a 500 ng mL lo que representa una cantidad suficiente de concentracioacuten para
aplicaciones praacutecticas ya que este factor de crecimiento muestra actividades bioloacutegicas in vitro en
dosis muy bajas (5ndash20 ng ml) (Ortega-Oller et al 2015)
Figura 22 Anaacutelisis de electroforesis en gel de SDS-poliacrilamida (SDS-PAGE) en
condiciones reductoras de Nanopartiacuteculas de PLGA soacutelidas (NP de PLGA) y fracciones liacutequidas
(sobrenadante) de diferentes sistemas de NP Carril P proteiacutenas estaacutendares carril A NP-BMP2
(proteiacutena morfogeneacutetica oacutesea) carril B sobrenadante de NP-BMP2 despueacutes de la siacutentesis y
encapsulacioacuten de rhBMP-2 carril C NP despueacutes de la adsorcioacuten fiacutesica de BSA rhBMP-2 carril
D sobrenadante despueacutes de la adsorcioacuten fiacutesica de BSA (albuacutemina de suero bovino) rhBMP-2
en el sistema NP
Por otro lado resultoacute un segundo nanosistema modificando la forma en que rhBMP-2 es
incorporado en el nanoportador Hay varios ejemplos de adsorcioacuten superficial de diferentes
factores de crecimiento en micro y nanopartiacuteculas (La et al 2010) (Fu et al 2012) (Rahman et
al 2014) y la inmovilizacioacuten de la superficie sobre la encapsulacioacuten recientemente se ha
108
propuesto como una forma de modular la liberacioacuten posterior de biomoleacuteculas Este proceso que
depende de la lenta difusioacuten de las biomoleacuteculas a traveacutes de la matriz polimeacuterica estaacute en
consecuencia altamente influenciado por la interaccioacuten proteiacutena-poliacutemero (Pakulska et al 2016)
(Fu et al 2017) y degradacioacuten del poliacutemero (Mir Ahmed and Rehman 2017) (Ding and Zhu
2018) Por lo tanto este nuevo enfoque en el uso de NP de PLGA para el suministro de
biomoleacuteculas se exploroacute inmovilizando la proteiacutena rhBMP-2 en la superficie de las NP vaciacuteas
mediante una simple adsorcioacuten fiacutesica Se sabe que este proceso se rige por interacciones
electrostaacuteticas e hidrofoacutebicas entre las moleacuteculas de proteiacutenas y las superficies NP (Peula and de
las Nieves 1993)
Para esto los grupos cargados en la superficie la hidrofilia la carga neta de las moleacuteculas de
proteiacutena y las caracteriacutesticas del medio de adsorcioacuten son los paraacutemetros de referencia Por lo
tanto disentildeamos un experimento de co-adsorcioacuten en el que interactuacutea una mezcla de rhBMP-2 y
BSA (04 p p rhBMP-2 BSA) simultaacuteneamente con la superficie de NP de PLGA Las
albuacuteminas se usan habitualmente como proteiacutenas protectoras cuando los factores de crecimiento
se incorporan en las NPs de PLGA (Ortega-Oller et al 2015) (Zhang et al 2016) Ademaacutes una
distribucioacuten superficial de las moleacuteculas de BSA puede mejorar la estabilidad coloidal de las NP
a pH fisioloacutegico debido a su carga negativa neta bajo estas condiciones (Peula and de las Nieves
1994) La Figura 23 muestra un esquema del proceso de co-adsorcioacuten La eficiencia de adsorcioacuten
es superior al 95 y en el SDS-PAGE de la Figura 22 se pueden ver dos bandas caracteriacutesticas
de ambas proteiacutenas en el carril C correspondiente al nanosistema NP-BSA-BMP2
109
Figura 23 Esquema del proceso de adsorcioacuten de proteiacutenas para NP-BSA-BMP2
Sin embargo el carril D correspondiente a la ejecucioacuten del sobrenadante desde la
centrifugacioacuten del nanosistema despueacutes de los procesos de adsorcioacuten muestra la ausencia de
cualquier proteiacutena Este resultado se explica completamente teniendo en cuenta el pH del medio
(pH 50) cerca del punto isoeleacutectrico de BSA donde la adsorcioacuten de esta proteiacutena en
nanopartiacuteculas cargadas negativamente presenta un maacuteximo (Peula and de las Nieves 1993)
(Peula Hidalgo-Alvarez and de las Nieves 1995) La inmovilizacioacuten de rhBMP-2 en la superficie
cargada negativamente de las NPs demuestra que estaacuten favorecidos electrostaacuteticamente debido a
la carga neta positiva de esta proteiacutena a pH aacutecido y neutro
532Caracterizacioacuten de nanopartiacuteculas
21Tamantildeo de nanopartiacuteculas
Micrografiacuteas SEM y STEM (Figura 24) muestran que las muestras consisten en partiacuteculas
esfeacutericas de diaacutemetros diferentes (entre 150 y 450 nm) un rango similar al encontrado en un
trabajo anterior en el que las NP se cargaron con lisozima siguiendo un protocolo de siacutentesis
similar (Ortega-Oller et al 2017) En ese trabajo la teacutecnica DLS no pudo proporcionar una
distribucioacuten de tamantildeo confiable Por lo tanto la teacutecnica NTA fue directamente utilizada para
determinar el tamantildeo hidrodinaacutemico de las NP cargadas con BMP2
Las distribuciones de tamantildeo para NP vaciacuteas (NP) y cargadas con BMP (NP-BMP2) de NTA
(Figura 25 y videos S1 S2) fueron consistentes con las imaacutegenes SEM Se encontroacute que las
partiacuteculas con diaacutemetros entre 100 y 500 nm teniacutean la concentracioacuten de partiacuteculas maacutes alta en
110
alrededor de 200 nm La carga con BMP tuvo un efecto en la distribucioacuten del tamantildeo lo que
condujo a picos maacutes definidos Estas mediciones nos permitieron determinar la concentracioacuten de
partiacuteculas en la muestra medida 688 plusmn 009 x 10 8 pp mL y 519 plusmn 012 x10 8 pp mL para
nanosistemas NP y NP-BMP2 respectivamente Estos valores fueron utilizados (teniendo en
cuenta la dilucioacuten correspondiente) para controlar el nuacutemero de partiacuteculas antildeadidas en los
experimentos celulares
Figura 24 Micrografiacutea de microscopiacutea electroacutenica de barrido (SEM) de nanopartiacuteculas
cargadas con rhBMP-2 (NP-BMP2)
111
Figura 25 Distribucioacuten del diaacutemetro hidrodinaacutemico de NP (ciacuterculos) y NP-BMP2 (liacutenea
negra gruesa) medidos a pH 70 (tampoacuten de fosfato) por anaacutelisis de seguimiento de
nanopartiacuteculas (NTA)
bullMovilidad electrocineacutetica y estabilidad coloidal
La carga superficial de las nanopartiacuteculas se puede analizar mediante un estudio electrocineacutetico
midiendo la movilidad electroforeacutetica (microe) en diferentes condiciones La Figura 26 muestra la
movilidad electroforeacutetica y el potencial zeta evaluado para los tres nanosistemas NP NP- BMP2
y NP-BSA-BMP2 a baja fuerza ioacutenica y diferentes valores de pH La carga superficial eleacutectrica
de las NP reside en los grupos carboxiacutelicos de las no cubiertas por PLGA y moleacuteculas de aacutecido
desoxicoacutelico Estos grupos funcionalizados tambieacuten son uacutetiles debido a la posibilidad de una
vectorizacioacuten quiacutemica de la superficie para desarrollar nanoportadores de entrega dirigida
(Siafaka et al 2016)
Se confirmoacute previamente que la protonacioacuten de estos grupos de superficies aacutecidas a valores de
pH bajo su valor de pKa estaba estrechamente relacionado con una peacuterdida de carga superficial
y en consecuencia una reduccioacuten (en valor absoluto) de la movilidad electroforeacutetica del sistema
coloidal (Peula-Garciacutea Hidalgo-Alvarez and De Las Nieves 1997) (Manuel J Santander-Ortega
Lozano-Loacutepez et al 2010) Por lo general cuando las partiacuteculas coloidales estaacuten recubiertas por
Conce
ntr
acioacute
n d
e par
tiacutecu
las
(10
6 p
pm
L)
112
moleacuteculas de proteiacutena los valores de microe cambian notablemente en comparacioacuten con el mismo
valor de las superficies desnudas y estaacuten influenciadas por la carga eleacutectrica de las moleacuteculas de
proteiacutena adsorbidas (Peula-Garcia Hidaldo-Alvarez and De las Nieves 1997) (Santander-Ortega
Bastos-Gonzalez and Ortega-Vinuesa 2007) El comportamiento electrocineacutetico del sistema NP-
BMP2 sigue siendo similar al de NP y la encapsulacioacuten de rhBMP-2 no afecta la distribucioacuten de
carga superficial DAngelo y colaboradores informaron de un resultado similar al encapsular
diferentes factores de crecimiento en nanopartiacuteculas de mezcla de PLGA-poloxaacutemero en la
misma proporcioacuten p p de proteiacutena poliacutemero (drsquoAngelo et al 2010) Esto puede deberse a la
baja cantidad de proteiacutena encapsulada y su distribucioacuten en la parte interna de las NP (lejos de la
superficie) En nuestro sistema la distribucioacuten interna puede verse favorecida por las condiciones
de encapsulacioacuten donde el pH baacutesico (pH 120) del agua contiene rhBMP-2 que permite una carga
negativa de estas moleacuteculas de proteiacutena evitando asiacute su interaccioacuten electrostaacutetica especiacutefica con
grupos aacutecidos de las NP
113
Figura 26 Movilidad electroforeacutetica y potencial zeta versus pH en medios de baja salinidad
(fuerza ioacutenica igual a 0002 M) para los diferentes nanosistemas (cuadrado negro) NP
(triaacutengulo azul) NP-BMP2 (ciacuterculo rojo) NP-BSA-BMP2
La distribucioacuten electrocineacutetica para el sistema NP-BSA-BMP2 cambia radicalmente Como se
mostroacute anteriormente la eficiencia de adsorcioacuten muy alta conduce a NP con ambas proteiacutenas
adsorbidas alrededor de su superficie Esta situacioacuten estaacute estrechamente relacionada con los
valores microe de la Figura 26 Teniendo en cuenta la relacioacuten p p entre proteiacutenas adsorbidas (250
veces mayor para BSA) las moleacuteculas de albuacutemina modulan el comportamiento a valores de pH
por debajo de su punto isoeleacutectrico (pI 47) donde la carga neta positiva BSA enmascara la carga
superficial original de las NP e incluso cambia sus valores originales a valores positivos Esto es
un resultado tiacutepico encontrado para estas partiacuteculas coloidales que cubren proteiacutenas (Peula
Hidalgo-Alvarez and de las Nieves 1995) (Peula JM Callejas J de las Nieves 1994) A
valores de pH neutros y baacutesicos las moleacuteculas de BSA tienen una carga neta negativa y la ligera
disminucioacuten en los valores absolutos microe podriacutea ser debido a la reduccioacuten de la carga superficial
neta negativa de los NP que pueden estar protegidos al menos en una pequentildea parte por la carga
positiva de las moleacuteculas de rhBMP-2 bajo su punto isoeleacutectrico baacutesico (pI 90)
Pote
nci
al z
eta
(mV
)
114
La estabilidad coloidal para los diferentes nanosistemas (NP NP-BMP2 y NP-BSA-BMP2)
fue determinada mediante el anaacutelisis de las distribuciones de tamantildeo en varios medios (PB PBS
y DMEM) en diferentes tiempos despueacutes de la siacutentesis (0 1 y 5 diacuteas) Se encontraron
distribuciones de tamantildeo similares a las originales para las dos formulaciones NP y NP-BMP2
en todos los medios analizados Este resultado fue similar al encontrado previamente para estos
tipos de NP que encapsulan la lisozima (Ortega-Oller et al 2017) en el que la combinacioacuten de
las interacciones electrostaacuteticas y esteacutericas generadas por grupos quiacutemicos superficiales de NP
confieren estabilidad al mecanismo que evita la agregacioacuten coloidal (Manuel J Santander-Ortega
Csaba et al 2010) La disminucioacuten del valor absoluto del potencial zeta para el sistema NP-
BSA-BMP2 como consecuencia de la distribucioacuten de proteiacutenas en la superficie no afecta su
estabilidad coloidal Este sistema tambieacuten mantiene la misma distribucioacuten de tamantildeos en los
diferentes medios Se acepta comuacutenmente que un potencial zeta superior a +30 o 30 mV daraacute
lugar a un sistema coloidal estable (Sun 2016) y el valor potencial zeta para NP-BSA-BMP2 es
superior a 30 mV La estabilidad coloidal en PBS y DMEM tiacutepicamente medios utilizados para
el desarrollo de interacciones celulares o scaffolds respectivamente asegura el uso potencial de
estos nanosistemas para entornos de vida in vitro o in vivo Ademaacutes estos sistemas mantuvieron
su tamantildeo bajo almacenamiento en PB a 4 deg C durante al menos 1 mes (datos no mostrados) lo
que demuestra que es un medio adecuado para el almacenamiento de muestras
bullLiberacioacuten de proteiacutenas
Uno de los principales problemas para los micro o nanosistemas de suministro de faacutermacos
PLGA es encontrar el patroacuten de liberacioacuten apropiado para las moleacuteculas de proteiacutenas encapsuladas
unidas Un amplio espectro de formulaciones modula esta propiedad mediante el uso de
diferentes tipos de procesos de siacutentesis poliacutemeros PLGA copoliacutemeros y estabilizadores (Mir
Ahmed and Rehman 2017) (Ortega-Oller et al 2015) Una limitacioacuten y control adecuados en la
liberacioacuten de estallido es fundamental para las BMP a fin de garantizar una liberacioacuten continua a
largo plazo que favorecida por la degradacioacuten del poliacutemero proporcione una mejor accioacuten in vivo
115
para impulsar la regeneracioacuten de hueso y cartiacutelago (Begam et al 2017) Por lo tanto previamente
desarrollamos un nanosistema PLGA dual para la liberacioacuten controlada a corto plazo donde la
difusioacuten de proteiacutenas y la interaccioacuten proteiacutena-poliacutemero son los principales factores que rigen este
proceso (Ortega-Oller et al 2017) En el presente trabajo los nanosistemas NP-BMP2 y NP-
BSA-BMP2 representan dos formas diferentes en las que se incorporoacute rhBMP-2 en el
nanoportador La Figura 27a muestra la liberacioacuten acumulativa de ambas proteiacutenas rhBMP-2 y
BSA para diferentes sistemas en funcioacuten del tiempo en un periacuteodo a corto plazo (7 diacuteas) La
proteiacutena rhBMP-2 encapsulada alcanza una cantidad liberada de alrededor del 30 de la proteiacutena
encapsulada inicial mientras que la rhBMP-2 adsorbida a pesar de su distribucioacuten superficial es
tres veces menor Sin embargo BSA muestra cantidades liberadas de hasta el 80 de los
adsorbidos iniciales En todos los casos las barras de error corresponden a las desviaciones
estaacutendar de tres experimentos independientes En estas condiciones el factor de crecimiento
encapsulado en NP-BMP2 presenta un patroacuten de liberacioacuten similar al encontrado previamente con
la misma formulacioacuten pero usando lisozima como proteiacutena (Ortega-Oller et al 2017) El
poloxaacutemero en la fase acuosa del proceso de siacutentesis puede ser clave para modular las
interacciones proteicas interfaciales especiacuteficas y no especiacuteficas (Del Castillo-Santaella et al
2019) Por lo tanto la relacioacuten entre la interaccioacuten proteiacutena-poliacutemero y la difusioacuten de proteiacutenas
parece estar bien equilibrada evitando un estallido inicial excesivo y al mismo tiempo
manteniendo el flujo de proteiacutenas necesario para liberar alrededor de un tercio de la rhBMP-2
encapsulada en 7 diacuteas Aunque se ha informado ampliamente de un estallido inicial excesivo para
los NP de PLGA relacionados con moleacuteculas de proteiacutenas cercanas a la superficie (Ding and Zhu
2018) esta situacioacuten no aparecioacute para el sistema NP-BMP2 siendo esto consistente con el
comportamiento electrocineacutetico que no mostroacute la presencia de proteiacutena cerca de la superficie La
literatura ofrece algunos ejemplos con liberacioacuten reducida a corto plazo de BMP-2 utilizando maacutes
copoliacutemeros PLGA-PEG hidroacutefilos (Kirby et al 2011) o un proceso de siacutentesis diferente (Chang
et al 2017)
116
El rendimiento de la liberacioacuten del sistema NP-BSA-BMP2 que tambieacuten se muestra en la
Figura 27a presenta diferencias notables El perfil electrocineacutetico ha justificado previamente la
ubicacioacuten de la superficie de BSA y rhBMP-2 en la superficie lo que podriacutea conducir a una
liberacioacuten raacutepida de ambas proteiacutenas Sin embargo los resultados de la Figura 27a y 27b muestran
esta tendencia solo para la proteiacutena BSA que se libera de las NP con aproximadamente el 20
de la cantidad inicial restante despueacutes de siete diacuteas Sin embargo hasta el 90 de la carga inicial
de proteiacutena rhBMP-2 a diferencia de BSA permanece unida a la superficie La superficie NP con
grupos hidrofiacutelicos forma moleacuteculas de poloxaacutemero y una carga negativa debido a la abundante
presencia de grupos carboxiacutelicos (grupos terminales de PLGA y moleacuteculas de aacutecido desoxicoacutelico)
favorecen un proceso de desorcioacuten para BSA cuyas moleacuteculas tienen una carga negativa en
condiciones de liberacioacuten (pH fisioloacutegico) Esto concuerda con los resultados de otros autores
que incluso despueacutes de encapsular BSA en mezclas de PLGA-poloxaacutemero NP logroacute una
descarga de liberacioacuten raacutepida por encima del 40 o 50 de la cantidad inicial de proteiacutena (Manuel
J Santander-Ortega Csaba et al 2010) Ademaacutes la coencapsulacioacuten de albuacuteminas con factores
de crecimiento podriacutea afectar fuertemente su perfil de liberacioacuten causando un estallido inicial
(Balmayor et al 2009) (drsquoAngelo et al 2010) De lo contrario la atraccioacuten electrostaacutetica
especiacutefica entre las moleacuteculas rhBMP-2 positivas y los grupos de superficie negativos ralentiza la
liberacioacuten a corto plazo de esta proteiacutena Este resultado estaacute de acuerdo con la baja liberacioacuten de
BMP adsorbida encontrada previamente usando micro y nanopartiacuteculas de PLGA con grupos
terminales de aacutecido sin tapar (Pakulska et al 2016) (Schrier and DeLuca 2001) Por lo tanto la
combinacioacuten de diferentes meacutetodos para atrapar BMP-2 dentro y alrededor de NP muestra la
posibilidad de lograr una liberacioacuten controlada adecuadamente equilibrando las interacciones
entre poliacutemeros estabilizadores y proteiacutenas
117
(a)
(b)
Figura 27 (a) Liberacioacuten acumulativa de rhBMP-2 para sistemas NP-BMP2 (cuadrado
negro) y NP-BSA-BMP2 (ciacuterculo rojo) y liberacioacuten acumulativa de BSA para el sistema NP-
BSA-BMP2 (triaacutengulo azul) incubado por diferentes tiempos a 37ordmC en tampoacuten de fosfato salino
(pH 74) (b) Anaacutelisis de SDS-PAGE en condiciones reductoras de fraccioacuten soacutelida de NP-BSA-
Lib
erac
ioacuten a
cum
ula
tiva
()
Tiempo (horas)
118
BMP2 despueacutes de la liberacioacuten en diferentes momentos en los que el nuacutemero de cada carril
corresponde al tiempo en horas
533Actividad bioloacutegica e interacciones
bullMigracioacuten Celular
La migracioacuten celular es el primer y necesario paso en la regeneracioacuten de tejidos (Padial-Molina
OrsquoValle et al 2015) Por lo tanto un agente regenerativo debe acelerar la migracioacuten celular o
al menos no interferir con ella En el presente estudio no encontramos diferencias entre los
grupos las dosis y el control en teacuterminos de cierre de un aacuterea rayada (ANOVA con la prueba de
comparaciones muacuteltiples de Tukey) (Figura 28) En contraste con nuestros hallazgos los datos
publicados anteriormente sugieren un efecto positivo de BMP-2 en la migracioacuten celular (Inai et
al 2008) (Gamell et al 2008) Sin embargo en esos estudios las dosis aplicadas y los tipos de
ceacutelulas fueron diferentes a los experimentos actuales Utilizamos dosis maacutes bajas de BMP-2 para
evaluar si incluso a dosis bajas BMP-2 podriacutea proporcionar beneficios si se protegiera en un
sistema de nanopartiacuteculas Como se mencionoacute no demostramos ninguacuten efecto negativo del
sistema en la migracioacuten celular Sin embargo nuestros resultados respaldan la idea de que la
actividad de BMP-2 estaacute mediada por la activacioacuten de la viacutea de la fosfoinositida 3-quinasa (PI3K)
un grupo comuacuten de moleacuteculas de sentildealizacioacuten que participan en varios procesos con BMP-2 y
otras moleacuteculas (Padial-Molina Volk and Rios 2014) (Gamell et al 2008) Tambieacuten debe
mencionarse que el plazo de un ensayo de migracioacuten es corto Por lo tanto las ventajas potenciales
de un sistema de liberacioacuten controlada como el que se estaacute estudiando podriacutean ser limitadas Es
decir la liberacioacuten de BMP-2 de las nanopartiacuteculas como se demuestra en la Figura 27 se limita
a las primeras 48 h Por lo tanto se podriacutea hipotetizar un efecto positivo sostenido sobre la
actividad migratoria a lo largo del tiempo
119
Figura 28 Ensayo de migracioacuten Porcentaje de cierre del aacuterea rayada a las 24 y 48 h en
diferentes grupos y dosis
bullProliferacioacuten celular
La proliferacioacuten es otra de las actividades celulares requeridas para la regeneracioacuten de tejidos
Sin embargo esta propiedad debe equilibrarse con la migracioacuten y la diferenciacioacuten y no aumentar
las tres caracteriacutesticas al mismo tiempo y con las mismas proporciones (Friedrichs et al 2011)
De hecho seguacuten los informes cuando una dosis de BMP-2 induce una mayor proliferacioacuten
disminuye la diferenciacioacuten (Hrubi et al 2018) Esta propiedad ha sido ampliamente analizada
pero las discrepancias auacuten se pueden detectar en la literatura Por lo tanto Kim y colaboradores
analizoacute diferentes dosis de BMP-2 y su efecto sobre la proliferacioacuten celular y la apoptosis Se
confirmoacute in vitro que las dosis altas pero auacuten maacutes bajas que las utilizadas cliacutenicamente reducen
la proliferacioacuten celular y aumentan la apoptosis (Kim Oxendine and Kamiya 2013) Esto debe
ser evitado Hemos encontrado que aunque el BMP-2 libre no induce una proliferacioacuten mayor
que el control en ninguna de las dosis aplicadas ni en los puntos de tiempo (ANOVA con la prueba
0
10
20
30
40
50
60
70
80
90
100
0 h 24 h 48 h
o
f sc
ratc
hed
are
a
Time point
Control
BMP2-125 ngmL
BMP2-25 ngmL
BMP2-5 ngmL
NP-BMP2-125 ngmL
NP-BMP2-25 ngmL
NP-BMP2-5 ngmL
NP-BSA-BMP2-125 ngmL
NP-BSA-BMP2-25 ngmL
NP-BSA-BMP2-5 ngmL
d
e aacuter
ea r
ayad
a
Tiempo
120
de comparaciones muacuteltiples de Tukey) la misma cantidad de BMP-2 encapsulada o adsorbida en
nanopartiacuteculas de PLGA aumenta la proliferacioacuten siendo esto estadiacutesticamente significativo
cuando se usa una dosis de 25 ng mL o maacutes (ANOVA con prueba de comparaciones muacuteltiples
de Tukey) (Figura 29) Estas dosis son auacuten maacutes bajas que las sugeridas en estudios anteriores
Aparte de esa diferencia todaviacutea se logroacute un efecto positivo sobre la proliferacioacuten Ademaacutes
siguiendo el patroacuten de lanzamiento de la Figura 27 se espera que se libere maacutes BMP-2 con el
tiempo maacutes allaacute del marco de tiempo de 7 diacuteas Por lo tanto tambieacuten podriacutea esperarse un efecto
de induccioacuten sostenido hasta la confluencia completa del cultivo celular
Figura 29 Proliferacioacuten de ceacutelulas del estroma mesenquimatoso humano (MSC) medida por
absorbancia de sulforamida (SRB) Los resultados se normalizaron a T0 en cada grupo
T0 T1 T2 T3 T4 T6 T70
2
4
6
Time point
No
rmalized
Ab
so
rban
ce
NP-BSA-BMP2-125 ngmL
NP-BSA-BMP2-25 ngmL
NP-BSA-BMP2-5 ngmL
NP-BMP2-125 ngmL
NP-BMP2-25 ngmL
NP-BMP2-5 ngmL
BMP2-125 ngmL
BMP2-25 ngmL
BMP2-5 ngmL
Control
Abso
rban
cia
norm
aliz
ada
Tiempo
121
bullDiferenciacioacuten osteogeacutenica
Se ha confirmado que la diferenciacioacuten celular inducida por BMP-2 necesita la presencia de
componentes osteoinductores permisivos En particular se ha demostrado que el beta-
glicerofosfato ejerce un efecto sineacutergico con BMP-2 para inducir la diferenciacioacuten celular (Hrubi
et al 2018) Por lo tanto para probar la diferenciacioacuten osteogeacutenica analizamos la expresioacuten del
ARNm de ALP Se encontroacute que la actividad maacutexima de ALP se produce 10 diacuteas despueacutes de la
estimulacioacuten con micropartiacuteculas basadas en PLGA que contienen BMP-2 en coencapsulacioacuten
con albuacutemina seacuterica humana (Kirby et al 2011) Aunque otras pruebas podriacutean haberse utilizado
para reforzar nuestros hallazgos se sabe que ALP modula la deposicioacuten de noacutedulos mineralizados
lo que indica actividad osteoblaacutestica Para todo esto complementamos los medios de
diferenciacioacuten con Beta-glicerofosfato y BMP-2 NP-BMP2 o NP-BSA-BMP2 libres durante 4 y
7 diacuteas para poder capturar la dinaacutemica temprana de la expresioacuten del gen En nuestro estudio
identificamos un aumento en la expresioacuten de ALP en todos los grupos desde el diacutea 4 hasta el diacutea
7 (Figura 30) Aunque ALP en el diacutea 7 en el grupo BMP-2 parece ser maacutes alto que en los otros
dos grupos el cambio no resultoacute significativo De hecho las diferencias entre los grupos no fueron
estadiacutesticamente significativas en ninguacuten periacuteodo de tiempo Sin embargo cabe destacar que el
aumento no fue significativo dentro del grupo BMP-2 (p = 0141 prueba t de Student) pero fue
significativo dentro de los otros dos grupos (p = 0025 y p = 0003 NP-BMP2 y NP-BSA- Grupos
BMP2 respectivamente) Esto nuevamente podriacutea tomarse como una confirmacioacuten de la
liberacioacuten sostenida de la proteiacutena del sistema de nanopartiacuteculas maacutes allaacute de los puntos temporales
anteriores
Esto y los estudios de migracioacuten y proliferacioacuten descritos a continuacioacuten nos llevan a confirmar
que el sistema propuesto puede mantener una liberacioacuten adecuada de BMP-2 a lo largo del tiempo
manteniendo un efecto positivo en la migracioacuten y proliferacioacuten celular con dosis iniciales
reducidas de BMP-2 El hecho de que se evite el estallido inicial excesivo es importante para la
aplicacioacuten de esta nanotecnologiacutea en la regeneracioacuten oacutesea como en la odontologiacutea De esta
122
manera los efectos negativos de las altas dosis iniciales de BMP-2 se evitan al mismo tiempo que
la moleacutecula estaacute protegida contra la desnaturalizacioacuten dentro del NP Por lo tanto los efectos del
regenerador se mantienen con el tiempo Los experimentos in vitro mostraron que las NP de
PLGA cargadas con BMP-2 son los nanoportadores con el mejor perfil de liberacioacuten a corto plazo
sin una explosioacuten inicial y con una liberacioacuten moderada y sostenida de proteiacutena activa antes del
inicio de la degradacioacuten del poliacutemero Por lo tanto la actividad bioloacutegica es positiva sin
interaccioacuten negativa con la migracioacuten o la proliferacioacuten sino maacutes bien la induccioacuten de la
diferenciacioacuten celular a traveacutes de la expresioacuten de ALP
Figura 30 Cambio de pliegue relativo en la expresioacuten de ARNm de ALP (grupo de control
BMP2 a los 4 diacuteas) = Importancia estadiacutestica de la comparacioacuten a lo largo del tiempo (p =
0025 y p = 0003 prueba t del estudiante grupos NP-BMP2 y NP-BSA-BMP2)
Los experimentos de liberacioacuten in vitro muestran un patroacuten adecuado de administracioacuten a corto
plazo que al mismo tiempo preserva la bioactividad de la biomoleacutecula encapsulada Ademaacutes la
distribucioacuten de tamantildeo de nanopartiacutecula encontrada para este nanosistema W-F68 permite la
Tiempo
Cam
bio
de
pli
egue
123
posibilidad de una administracioacuten de proteiacutena dual externa e intracelular como se ha demostrado
mediante experimentos celulares in vitro Esta nueva formulacioacuten se utilizaraacute en futuros estudios
para encapsular y administrar factores de crecimiento in vitro e in vivo con el fin de explotar el
potencial terapeacuteutico de este nanosistema
Se ha puesto de manifiesto la necesidad de optimizacioacuten de los meacutetodos y componentes para
equilibrar la estructura y morfologiacutea de las micropartiacuteculas nanopartiacuteculas de PLGA logrando
de esta forma una alta eficiencia de encapsulacioacuten de BMP-2 y buscando un objetivo principal el
control de la entrega la reduccioacuten de la descarga inicial para alcanzar un perfil de liberacioacuten de
la proteiacutena sostenido en el tiempo preservando la actividad bioloacutegica y dirigieacutendola a ceacutelulas
diana para minimizar la cantidad cliacutenica de proteiacutena necesaria permitiendo al mismo tiempo una
correcta regeneracioacuten del tejido oacuteseo
En consecuencia otro reto futuro es conseguir el direccionamiento especiacutefico de estas nano-
esferas de PLGA cargadas de agentes activos Este aspecto se puede desarrollar mediante el uso
de unos ligandos que reconozcan especiacuteficamente los tipos o liacuteneas celulares a la que queremos
dirigir la liberacioacuten de biomoleacuteculas encapsuladas El uso de nanopartiacuteculas con una unioacuten
covalente de diferentes ligandos da lugar a una teacutecnica con un alto potencial de administracioacuten
que permite a la ingenieriacutea tisular un gran avance en cuanto a la distribucioacuten y administracioacuten de
diferentes faacutermacos o biomoleacuteculas mejorando asiacute las funciones bioloacutegicas o regenerativas
celulares
Los anticuerpos especiacuteficos que reconocen los receptores de superficie celular pueden unirse
covalentemente a la superficie de nuestras nanopartiacuteculas de PLGA dando lugar a una ldquoinmuno-
nanopartiacuteculardquo Para que esta unioacuten se produzca sin ninguacuten inconveniente en muchas ocasiones
hay que hacer uso de agentes estabilizadores para proporcionar estabilidad coloidal a las
nanopartiacuteculas sin que lleguen a afectar al enlace establecido entre los anticuerpos especiacuteficos de
los receptores celulares y las nanopartiacuteculas que es donde nos encontramos hoy diacutea y donde
124
muchos investigadores siguen haciendo avances cada diacutea entorno a la mejora de estos enlaces y
de estas entregas celulares especificas a traveacutes de las ldquoinmuno-nanopartiacuteculasrdquo
125
6CONCLUSIONES
En respuesta al objetivo principal de este trabajo
Los nanosistemas de partiacuteculas polimeacutericas basados en PLGA son sistemas prometedores para la
administracioacuten espacial y temporalmente controlada de factores de crecimiento que promueven
el desarrollo celular la diferenciacioacuten y regeneracioacuten en ingenieriacutea oacutesea mediante su
incorporacioacuten junto a las ceacutelulas en estructuras soacutelidas o hidrogeles
En respuesta a los objetivos secundarios de este trabajo
bullHa sido posible optimizar la obtencioacuten de diferentes sistemas de NPs de PLGA mediante un
procedimiento de doble emulsioacuten con las propiedades superficiales adecuadas que proporcionan
estabilidad coloidal y grupos carboxilo superficiales para unir quiacutemicamente diferentes ligandos
especiacuteficos en respuesta al objetivo 1
bullSe ha optimizado una formulacioacuten W-F68 que basada en el procedimiento anterior permite
encapsular moleacuteculas hidrofiacutelicas como las proteiacutenas obteniendo un novedoso nanotransportador
de tamantildeo dual que preserva la actividad bioloacutegica de la proteiacutena modelo encapsulada (lisozima)
en respuesta al objetivo 2
bullEn respuesta al tercer objetivo el anaacutelisis de la interaccioacuten proteiacutena-surfactante muestra el
papel crucial del solvente orgaacutenico el surfactante la relacioacuten de volumen entre ambas fases y la
carga neta de la proteiacutena encapsulada sobre las caracteriacutesticas finales de las NPs transportadoras
y el patroacuten de liberacioacuten proteica
bullLas experiencias in vitro de suministro proteico muestran una difusioacuten bien equilibrada de la
proteiacutena evitando una descarga inicial excesiva manteniendo un flujo constante de liberacioacuten a
corto plazo y permitiendo un suministro dual extra e intracelular sin citotoxicidad apreciable en
respuesta a los objetivos 4 y 5
126
bullSe ha desarrollado un nanosistema transportador de BMP2 sobre la base de un sistema modelo
formulado previamente mediante un procedimiento de doble emulsioacuten que conduce a un sistema
de NPs con una distribucioacuten dual de tamantildeos y estabilidad coloidal y temporal adecuada para
aplicaciones bioloacutegicas en respuesta al objetivo 6
bullIn vitro el Sistema con BMP2 encapsulada presenta un patroacuten de liberacioacuten en el corto plazo
7 diacuteas que muestra un suministro moderado y sostenido de proteiacutena bioloacutegicamente activa en
respuesta al objetivo 7
bullIn vitro la actividad bioloacutegica a nivel celular muestra mediante el anaacutelisis de la expresioacuten de
ALP la capacidad de la BMP2 nanotransportada para inducir diferenciacioacuten celular sin incidencia
negativa en los procesos de migracioacuten y proliferacioacuten celular en respuesta al objetivo 8
127
7 CONFLICTO DE INTERESES
Los autores declaran no tener ninguacuten conflicto de intereses con ninguno de los productos
enumerados en el documento
8 RECURSOS ECONOacuteMICOS
Beca de investigacioacuten obtenida competitivamente y otorgada por la empresa de implantes
dentales ldquoMIS IBERICA SLrdquo
Financiacioacuten parcial otorgada 1- por la Consejeriacutea de Economiacutea Innovacioacuten Educacioacuten
Ciencia y Empleo de la Junta de Andaluciacutea (Espantildea) 2- los proyectos MAT2013-43922-R ndash
incluyendo soporte europeo FEDER - (MICINN Espantildea) 3- y los Grupos de Investigacioacuten
FQM-115 CTS-1028 CTS-138 y CTS- 583 (Junta de Andaluciacutea Espantildea)
128
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Arias J L et al (2015) lsquoNanobody conjugated PLGA nanoparticles for active targeting of
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Balmayor E R et al (2009) lsquoStarch-poly-epsilon-caprolactone microparticles reduce the
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Begam H et al (2017) lsquoStrategies for delivering bone morphogenetic protein for bone
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Boyne P J et al (2005) lsquoDe novo bone induction by recombinant human bone
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Boyne P and Jones S D (2004) lsquoDemonstration of the osseoinductive effect of bone
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Brown B N et al (2009) lsquoMacrophage phenotype and remodeling outcomes in response to
biologic scaffolds with and without a cellular componentrsquo Biomaterials 30(8) pp 1482ndash
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Brown B N et al (2011) lsquoComparison of three methods for the derivation of a biologic
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Cai C et al (2008) lsquoCharged nanoparticles as protein delivery systems a feasibility study
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Carragee E J Hurwitz E L and Weiner B K (2011) lsquoA critical review of recombinant
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Carreira A C et al (2014) lsquoBone morphogenetic proteins facts challenges and future
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Carreira Ana Claudia et al (2014) lsquoBone Morphogenetic Proteins structure biological
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Del Castillo-Santaella T et al (2019) lsquoInteraction of surfactant and protein at the OW
interface and its effect on colloidal and biological properties of polymeric nanocarriersrsquo
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Chan J M et al (2009) lsquoPLGA-lecithin-PEG core-shell nanoparticles for controlled drug
deliveryrsquo Biomaterials 30(8) pp 1627ndash1634 doi 101016jbiomaterials200812013
Chang H-C et al (2017) lsquoBone morphogenetic protein-2 loaded poly(DL-lactide-co-
glycolide) microspheres enhance osteogenic potential of gelatinhydroxyapatitebeta-
tricalcium phosphate cryogel composite for alveolar ridge augmentationrsquo Journal of the
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101016jjfma201701005
Chen G Deng C and Li Y-P (2012) lsquoTGF-beta and BMP signaling in osteoblast
differentiation and bone formationrsquo International journal of biological sciences 8(2) pp
272ndash288 doi 107150ijbs2929
Cheng J et al (2007) lsquoFormulation of functionalized PLGA-PEG nanoparticles for in vivo
targeted drug deliveryrsquo Biomaterials 28(5) pp 869ndash876 doi
101016jbiomaterials200609047
Chou L Y T Ming K and Chan W C W (2011) lsquoStrategies for the intracellular delivery of
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Chung V H-Y et al (2012) lsquoEngineered autologous bone marrow mesenchymal stem cells
alternative to cleft alveolar bone graft surgeryrsquo The Journal of craniofacial surgery 23(5)
pp 1558ndash1563 doi 101097SCS0b013e31825e4e30
Chung Y-I et al (2007) lsquoEnhanced bone regeneration with BMP-2 loaded functional
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nanoparticle-hydrogel complexrsquo Journal of controlled release official journal of the
Controlled Release Society 121(1ndash2) pp 91ndash99 doi 101016jjconrel200705029
Cleland J L (1997) lsquoProtein delivery from biodegradable microspheresrsquo Pharmaceutical
biotechnology 10 pp 1ndash43 doi 1010070-306-46803-4_1
Csaba N et al (2004) lsquoDesign and characterisation of new nanoparticulate polymer blends
for drug deliveryrsquo Journal of biomaterials science Polymer edition 15(9) pp 1137ndash1151
doi 1011631568562041753098
Csaba N et al (2005) lsquoPLGApoloxamer and PLGApoloxamine blend nanoparticles new
carriers for gene deliveryrsquo Biomacromolecules 6(1) pp 271ndash278 doi
101021bm049577p
Csaba N Garcia-Fuentes M and Alonso M J (2006) lsquoThe performance of nanocarriers for
transmucosal drug deliveryrsquo Expert opinion on drug delivery 3(4) pp 463ndash478 doi
1015171742524734463
drsquoAngelo I et al (2010) lsquoNanoparticles based on PLGApoloxamer blends for the delivery of
proangiogenic growth factorsrsquo Molecular pharmaceutics 7(5) pp 1724ndash1733 doi
101021mp1001262
Danhier F et al (2012) lsquoPLGA-based nanoparticles an overview of biomedical
applicationsrsquo Journal of controlled release official journal of the Controlled Release Society
161(2) pp 505ndash522 doi 101016jjconrel201201043
Deschaseaux F Sensebe L and Heymann D (2009) lsquoMechanisms of bone repair and
regenerationrsquo Trends in molecular medicine 15(9) pp 417ndash429 doi
101016jmolmed200907002
Devine J G et al (2012) lsquoThe use of rhBMP in spine surgery is there a cancer riskrsquo
Evidence-based spine-care journal 3(2) pp 35ndash41 doi 101055s-0031-1298616
Ding D and Zhu Q (2018) lsquoRecent advances of PLGA micronanoparticles for the delivery
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Ertl B et al (2000) lsquoLectin-mediated bioadhesion preparation stability and caco-2 binding
of wheat germ agglutinin-functionalized Poly(DL-lactic-co-glycolic acid)-microspheresrsquo
Journal of drug targeting 8(3) pp 173ndash184 doi 10310910611860008996863
Fang D-L et al (2014) lsquoDevelopment of lipid-shell and polymer core nanoparticles with
water-soluble salidroside for anti-cancer therapyrsquo International journal of molecular
sciences 15(3) pp 3373ndash3388 doi 103390ijms15033373
Farace C et al (2016) lsquoImmune cell impact of three differently coated lipid nanocapsules
pluronic chitosan and polyethylene glycolrsquo Scientific reports 6 p 18423 doi
101038srep18423
Feczkoacute T Toacuteth J and Gyenis J (2008) lsquoComparison of the preparation of PLGA-BSA nano-
and microparticles by PVA poloxamer and PVPrsquo Colloids and Surfaces A Physicochemical
and Engineering Aspects 319(1ndash3) pp 188ndash195 doi 101016jcolsurfa200707011
Feng S and Huang G (2001) lsquoEffects of emulsifiers on the controlled release of paclitaxel
(Taxol) from nanospheres of biodegradable polymersrsquo Journal of controlled release official
journal of the Controlled Release Society 71(1) pp 53ndash69 doi 101016s0168-
3659(00)00364-3
Fraylich M et al (2008) lsquoPoly(DL-lactide-co-glycolide) dispersions containing pluronics
from particle preparation to temperature-triggered aggregationrsquo Langmuir the ACS
journal of surfaces and colloids 24(15) pp 7761ndash7768 doi 101021la800869u
Fredenberg S et al (2011) lsquoThe mechanisms of drug release in poly(lactic-co-glycolic acid)-
based drug delivery systems--a reviewrsquo International journal of pharmaceutics 415(1ndash2)
pp 34ndash52 doi 101016jijpharm201105049
Friedrichs M et al (2011) lsquoBMP signaling balances proliferation and differentiation of
134
muscle satellite cell descendantsrsquo BMC cell biology 12 p 26 doi 1011861471-2121-12-
26
Froum S J et al (2006) lsquoComparison of mineralized cancellous bone allograft (Puros) and
anorganic bovine bone matrix (Bio-Oss) for sinus augmentation histomorphometry at 26
to 32 weeks after graftingrsquo The International journal of periodontics amp restorative dentistry
26(6) pp 543ndash551
Fu C et al (2017) lsquoEnhancing Cell Proliferation and Osteogenic Differentiation of MC3T3-
E1 Pre-osteoblasts by BMP-2 Delivery in Graphene Oxide-Incorporated PLGAHA
Biodegradable Microcarriersrsquo Scientific reports 7(1) p 12549 doi 101038s41598-017-
12935-x
Fu R et al (2013) lsquoEffectiveness and harms of recombinant human bone morphogenetic
protein-2 in spine fusion a systematic review and meta-analysisrsquo Annals of internal
medicine 158(12) pp 890ndash902 doi 1073260003-4819-158-12-201306180-00006
Fu Y et al (2012) lsquoIn vitro sustained release of recombinant human bone morphogenetic
protein-2 microspheres embedded in thermosensitive hydrogelsrsquo Die Pharmazie 67(4) pp
299ndash303
Galindo-Moreno P et al (2007) lsquoEvaluation of sinus floor elevation using a composite bone
graft mixturersquo Clinical oral implants research 18(3) pp 376ndash382 doi 101111j1600-
0501200701337x
Galindo-Moreno P et al (2011) lsquoEffect of anorganic bovine bone to autogenous cortical
bone ratio upon bone remodeling patterns following maxillary sinus augmentationrsquo Clinical
oral implants research 22(8) pp 857ndash864 doi 101111j1600-0501201002073x
Gamell C et al (2008) lsquoBMP2 induction of actin cytoskeleton reorganization and cell
migration requires PI3-kinase and Cdc42 activityrsquo Journal of cell science 121(Pt 23) pp
3960ndash3970 doi 101242jcs031286
135
Gaudana R et al (2013) lsquoDesign and evaluation of a novel nanoparticulate-based
formulation encapsulating a HIP complex of lysozymersquo Pharmaceutical development and
technology 18(3) pp 752ndash759 doi 103109108374502012737806
Ghaderi R and Carlfors J (1997) lsquoBiological activity of lysozyme after entrapment in
poly(dl-lactide-co-glycolide)-microspheresrsquo Pharmaceutical research 14(11) pp 1556ndash
1562 doi 101023a1012122200381
Giteau A et al (2008) lsquoHow to achieve sustained and complete protein release from PLGA-
based microparticlesrsquo International journal of pharmaceutics 350(1ndash2) pp 14ndash26 doi
101016jijpharm200711012
Gref R et al (1994) lsquoBiodegradable long-circulating polymeric nanospheresrsquo Science (New
York NY) 263(5153) pp 1600ndash1603 doi 101126science8128245
Hans M L and Lowman A M (2002) lsquoBiodegradable nanoparticles for drug delivery and
targetingrsquo Current Opinion in Solid State and Materials Science 6(4) pp 319ndash327 doi
101016S1359-0286(02)00117-1
Hassan P A Rana S and Verma G (2015) lsquoMaking sense of Brownian motion colloid
characterization by dynamic light scatteringrsquo Langmuir the ACS journal of surfaces and
colloids 31(1) pp 3ndash12 doi 101021la501789z
Hines D J and Kaplan D L (2013) lsquoPoly(lactic-co-glycolic) acid-controlled-release systems
experimental and modeling insightsrsquo Critical reviews in therapeutic drug carrier systems
30(3) pp 257ndash276 doi 101615critrevtherdrugcarriersyst2013006475
Hong P et al (2013) lsquoEnhancement of bone consolidation in mandibular distraction
osteogenesis a contemporary review of experimental studies involving adjuvant
therapiesrsquo Journal of plastic reconstructive amp aesthetic surgery JPRAS 66(7) pp 883ndash895
doi 101016jbjps201303030
Houghton P et al (2007) lsquoThe sulphorhodamine (SRB) assay and other approaches to
136
testing plant extracts and derived compounds for activities related to reputed anticancer
activityrsquo Methods (San Diego Calif) 42(4) pp 377ndash387 doi 101016jymeth200701003
Hrubi E et al (2018) lsquoDiverse effect of BMP-2 homodimer on mesenchymal progenitors of
different originrsquo Human cell 31(2) pp 139ndash148 doi 101007s13577-018-0202-5
Inai K et al (2008) lsquoBMP-2 induces cell migration and periostin expression during
atrioventricular valvulogenesisrsquo Developmental biology 315(2) pp 383ndash396 doi
101016jydbio200712028
Iqbal M et al (2015) lsquoDouble emulsion solvent evaporation techniques used for drug
encapsulationrsquo International journal of pharmaceutics 496(2) pp 173ndash190 doi
101016jijpharm201510057
Jana Sougata and Jana Subrata (2017) lsquoNatural polymeric biodegradable nanoblend for
macromolecules deliveryrsquo in Recent Developments in Polymer Macro Micro and Nano Blends
Preparation and Characterisation Elsevier Inc pp 289ndash312 doi 101016B978-0-08-
100408-100010-8
Jeon O et al (2008) lsquoLong-term delivery enhances in vivo osteogenic efficacy of bone
morphogenetic protein-2 compared to short-term deliveryrsquo Biochemical and biophysical
research communications 369(2) pp 774ndash780 doi 101016jbbrc200802099
Ji W et al (2012) lsquoLocal delivery of small and large biomolecules in craniomaxillofacial
bonersquo Advanced drug delivery reviews 64(12) pp 1152ndash1164 doi
101016jaddr201203003
Ji Y et al (2010) lsquoBMP-2PLGA delayed-release microspheres composite graft selection of
bone particulate diameters and prevention of aseptic inflammation for bone tissue
engineeringrsquo Annals of biomedical engineering 38(3) pp 632ndash639 doi 101007s10439-
009-9888-6
Jiang W et al (2005) lsquoBiodegradable poly(lactic-co-glycolic acid) microparticles for
137
injectable delivery of vaccine antigensrsquo Advanced drug delivery reviews 57(3) pp 391ndash410
doi 101016jaddr200409003
Kao D W K et al (2012) lsquoThe negative effect of combining rhBMP-2 and Bio-Oss on bone
formation for maxillary sinus augmentationrsquo The International journal of periodontics amp
restorative dentistry 32(1) pp 61ndash67
Katranji A Fotek P and Wang H-L (2008) lsquoSinus augmentation complications etiology
and treatmentrsquo Implant dentistry 17(3) pp 339ndash349 doi
101097ID0b013e3181815660
Kempen D H R et al (2008) lsquoRetention of in vitro and in vivo BMP-2 bioactivities in
sustained delivery vehicles for bone tissue engineeringrsquo Biomaterials 29(22) pp 3245ndash
3252 doi 101016jbiomaterials200804031
Kempen D H R et al (2009) lsquoEffect of local sequential VEGF and BMP-2 delivery on ectopic
and orthotopic bone regenerationrsquo Biomaterials 30(14) pp 2816ndash2825 doi
101016jbiomaterials200901031
Ki-Bum Lee Ani Solanki J Dongun Kim J J (2009) Nanomedicine Dynamic Integration of
Nanotechnology with Biomedical Science | Request PDF Available at
httpswwwresearchgatenetpublication254745458_Nanomedicine_Dynamic_Integrati
on_of_Nanotechnology_with_Biomedical_Science (Accessed 29 March 2020)
Kim H K W Oxendine I and Kamiya N (2013) lsquoHigh-concentration of BMP2 reduces cell
proliferation and increases apoptosis via DKK1 and SOST in human primary periosteal
cellsrsquo Bone 54(1) pp 141ndash150 doi 101016jbone201301031
Kim Y-H and Tabata Y (2015) lsquoDual-controlled release system of drugs for bone
regenerationrsquo Advanced drug delivery reviews 94 pp 28ndash40 doi
101016jaddr201506003
Kirby G T S et al (2011) lsquoPLGA-based microparticles for the sustained release of BMP-2rsquo
138
in European Cells and Materials AO Research Institute Davos p 24 doi
103390polym3010571
Kocbek P et al (2007) lsquoTargeting cancer cells using PLGA nanoparticles surface modified
with monoclonal antibodyrsquo Journal of controlled release official journal of the Controlled
Release Society 120(1ndash2) pp 18ndash26 doi 101016jjconrel200703012
Kok R J et al (1998) lsquoDrug delivery to the kidneys and the bladder with the low molecular
weight protein lysozymersquo Renal failure 20(2) pp 211ndash217 doi
10310908860229809045104
Kumar B et al (2017) lsquoRecent advances in nanoparticle-mediated drug deliveryrsquo Journal of
Drug Delivery Science and Technology Editions de Sante pp 260ndash268 doi
101016jjddst201707019
Kumar T R S Soppimath K and Nachaegari S K (2006) lsquoNovel delivery technologies for
protein and peptide therapeuticsrsquo Current pharmaceutical biotechnology 7(4) pp 261ndash
276 doi 102174138920106777950852
Kumari A Yadav S K and Yadav S C (2010) lsquoBiodegradable polymeric nanoparticles
based drug delivery systemsrsquo Colloids and surfaces B Biointerfaces 75(1) pp 1ndash18 doi
101016jcolsurfb200909001
La W-G et al (2010) lsquoThe efficacy of bone morphogenetic protein-2 depends on its mode
of deliveryrsquo Artificial organs 34(12) pp 1150ndash1153 doi 101111j1525-
1594200900988x
Lee J et al (2013) lsquoSinus augmentation using rhBMP-2ACS in a mini-pig model relative
efficacy of autogenous fresh particulate iliac bone graftsrsquo Clinical oral implants research
24(5) pp 497ndash504 doi 101111j1600-0501201102419x
Lee S-J et al (2017) lsquoDevelopment of Novel 3-D Printed Scaffolds With Core-Shell
Nanoparticles for Nerve Regenerationrsquo IEEE transactions on bio-medical engineering 64(2)
139
pp 408ndash418 doi 101109TBME20162558493
Li B et al (2009) lsquoThe effects of rhBMP-2 released from biodegradable
polyurethanemicrosphere composite scaffolds on new bone formation in rat femorarsquo
Biomaterials 30(35) pp 6768ndash6779 doi 101016jbiomaterials200908038
Liang C-C Park A Y and Guan J-L (2007) lsquoIn vitro scratch assay a convenient and
inexpensive method for analysis of cell migration in vitrorsquo Nature protocols 2(2) pp 329ndash
333 doi 101038nprot200730
LIN Y et al (2007) lsquoIn vitro Evaluation of Lysozyme-loaded Microspheres in
Thermosensitive Methylcellulose-based Hydrogel1 1 Supported by the National Natural
Science Foundation of China (No20576057) and Fundamental Research Foundation of
Tsinghua University (JCqn2005033)rsquo Chinese Journal of Chemical Engineering 15(4) pp
566ndash572 doi 101016S1004-9541(07)60125-6
Lochmann A et al (2010) lsquoThe influence of covalently linked and free polyethylene glycol
on the structural and release properties of rhBMP-2 loaded microspheresrsquo Journal of
controlled release official journal of the Controlled Release Society 147(1) pp 92ndash100 doi
101016jjconrel201006021
Loureiro J A et al (2016) lsquoCellular uptake of PLGA nanoparticles targeted with anti-amyloid
and anti-transferrin receptor antibodies for Alzheimerrsquos disease treatmentrsquo Colloids and
surfaces B Biointerfaces 145 pp 8ndash13 doi 101016jcolsurfb201604041
Luginbuehl V et al (2004) lsquoLocalized delivery of growth factors for bone repairrsquo European
journal of pharmaceutics and biopharmaceutics official journal of Arbeitsgemeinschaft fur
Pharmazeutische Verfahrenstechnik eV 58(2) pp 197ndash208 doi
101016jejpb200403004
M Padial-Molina P Galindo-Moreno G Aacute-M (2009) lsquoBiomimetic ceramics in implant
dentistryrsquo MINERVA BIOTECNOLOGICA 21 p 173
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Makadia H K and Siegel S J (2011) lsquoPoly Lactic-co-Glycolic Acid (PLGA) as Biodegradable
Controlled Drug Delivery Carrierrsquo Polymers 3(3) pp 1377ndash1397 doi
103390polym3031377
Maldonado-Valderrama J et al (2013) lsquoIn vitro digestion of interfacial protein structuresrsquo
Soft Matter 9(4) pp 1043ndash1053 doi 101039c2sm26843d
Mason S et al (2014) lsquoStandardization and safety of alveolar bone-derived stem cell
isolationrsquo Journal of dental research 93(1) pp 55ndash61 doi 1011770022034513510530
McClements D J (2018) lsquoEncapsulation protection and delivery of bioactive proteins and
peptides using nanoparticle and microparticle systems A reviewrsquo Advances in colloid and
interface science 253 pp 1ndash22 doi 101016jcis201802002
McKay W F Peckham S M and Badura J M (2007) lsquoA comprehensive clinical review of
recombinant human bone morphogenetic protein-2 (INFUSE Bone Graft)rsquo International
orthopaedics 31(6) pp 729ndash734 doi 101007s00264-007-0418-6
Meinel L et al (2001) lsquoStabilizing insulin-like growth factor-I in poly(DL-lactide-co-
glycolide) microspheresrsquo Journal of controlled release official journal of the Controlled
Release Society 70(1ndash2) pp 193ndash202 doi 101016s0168-3659(00)00352-7
Meng F T et al (2003) lsquoWOW double emulsion technique using ethyl acetate as organic
solvent effects of its diffusion rate on the characteristics of microparticlesrsquo Journal of
controlled release official journal of the Controlled Release Society 91(3) pp 407ndash416 doi
101016s0168-3659(03)00273-6
Mir M Ahmed N and Rehman A U (2017) lsquoRecent applications of PLGA based
nanostructures in drug deliveryrsquo Colloids and surfaces B Biointerfaces 159 pp 217ndash231
doi 101016jcolsurfb201707038
Misch C E (1987) lsquoMaxillary sinus augmentation for endosteal implants organized
alternative treatment plansrsquo The International journal of oral implantology implantologist
141
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Mohamed F and van der Walle C F (2008) lsquoEngineering biodegradable polyester particles
with specific drug targeting and drug release propertiesrsquo Journal of pharmaceutical sciences
97(1) pp 71ndash87 doi 101002jps21082
Morille M et al (2013) lsquoNew PLGA-P188-PLGA matrix enhances TGF-beta3 release from
pharmacologically active microcarriers and promotes chondrogenesis of mesenchymal
stem cellsrsquo Journal of controlled release official journal of the Controlled Release Society
170(1) pp 99ndash110 doi 101016jjconrel201304017
Mueller T D and Nickel J (2012) lsquoPromiscuity and specificity in BMP receptor activationrsquo
FEBS letters 586(14) pp 1846ndash1859 doi 101016jfebslet201202043
Myeroff C and Archdeacon M (2011) lsquoAutogenous bone graft donor sites and techniquesrsquo
The Journal of bone and joint surgery American volume 93(23) pp 2227ndash2236 doi
102106JBJSJ01513
Nair B P and Sharma C P (2012) lsquoPoly(lactide-co-glycolide)-laponite-F68 nanocomposite
vesicles through a single-step double-emulsion method for the controlled release of
doxorubicinrsquo Langmuir the ACS journal of surfaces and colloids 28(9) pp 4559ndash4564 doi
101021la300005c
Nevins M et al (1996) lsquoBone formation in the goat maxillary sinus induced by absorbable
collagen sponge implants impregnated with recombinant human bone morphogenetic
protein-2rsquo The International journal of periodontics amp restorative dentistry 16(1) pp 8ndash19
Oh S H Kim T H and Lee J H (2011) lsquoCreating growth factor gradients in three
dimensional porous matrix by centrifugation and surface immobilizationrsquo Biomaterials
32(32) pp 8254ndash8260 doi 101016jbiomaterials201107027
Ortega-Oller I et al (2015) lsquoBone Regeneration from PLGA Micro-Nanoparticlesrsquo BioMed
research international 2015 p 415289 doi 1011552015415289
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Ortega-Oller I et al (2017) lsquoDual delivery nanosystem for biomolecules Formulation
characterization and in vitro releasersquo Colloids and surfaces B Biointerfaces 159 pp 586ndash
595 doi 101016jcolsurfb201708027
Padial-Molina M et al (2012) lsquoMethods to validate tooth-supporting regenerative
therapiesrsquo Methods in molecular biology (Clifton NJ) 887 pp 135ndash148 doi 101007978-
1-61779-860-3_13
Padial-Molina M OrsquoValle F et al (2015) lsquoClinical Application of Mesenchymal Stem Cells
and Novel Supportive Therapies for Oral Bone Regenerationrsquo BioMed research
international 2015 p 341327 doi 1011552015341327
Padial-Molina M Rodriguez J C et al (2015) lsquoStandardized in vivo model for studying
novel regenerative approaches for multitissue bone-ligament interfacesrsquo Nature protocols
10(7) pp 1038ndash1049 doi 101038nprot2015063
Padial-Molina M et al (2019) lsquoExpression of Musashi-1 During Osteogenic Differentiation
of Oral MSC An In Vitro Studyrsquo International journal of molecular sciences 20(9) doi
103390ijms20092171
Padial-Molina M and Rios H F (2014) lsquoStem Cells Scaffolds and Gene Therapy for
Periodontal Engineeringrsquo Current Oral Health Reports 1(1) pp 16ndash25 doi
101007s40496-013-0002-7
Padial-Molina M Volk S L and Rios H F (2014) lsquoPeriostin increases migration and
proliferation of human periodontal ligament fibroblasts challenged by tumor necrosis factor
-alpha and Porphyromonas gingivalis lipopolysaccharidesrsquo Journal of periodontal research
49(3) pp 405ndash414 doi 101111jre12120
Paillard-Giteau A et al (2010) lsquoEffect of various additives and polymers on lysozyme
release from PLGA microspheres prepared by an sow emulsion techniquersquo European
journal of pharmaceutics and biopharmaceutics official journal of Arbeitsgemeinschaft fur
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Pharmazeutische Verfahrenstechnik eV 75(2) pp 128ndash136 doi
101016jejpb201003005
Pakulska M M et al (2016) lsquoEncapsulation-free controlled release Electrostatic adsorption
eliminates the need for protein encapsulation in PLGA nanoparticlesrsquo Science advances
2(5) p e1600519 doi 101126sciadv1600519
Pantazis P et al (2012) lsquoPreparation of siRNA-encapsulated PLGA nanoparticles for
sustained release of siRNA and evaluation of encapsulation efficiencyrsquo Methods in molecular
biology (Clifton NJ) 906 pp 311ndash319 doi 101007978-1-61779-953-2_25
Panyam J and Labhasetwar V (2003) lsquoDynamics of endocytosis and exocytosis of poly(DL-
lactide-co-glycolide) nanoparticles in vascular smooth muscle cellsrsquo Pharmaceutical
research 20(2) pp 212ndash220 doi 101023a1022219003551
Paolicelli P et al (2010) lsquoSurface-modified PLGA-based nanoparticles that can efficiently
associate and deliver virus-like particlesrsquo Nanomedicine (London England) 5(6) pp 843ndash
853 doi 102217nnm1069
Park J S et al (2013) lsquoMultilineage differentiation of human-derived dermal fibroblasts
transfected with genes coated on PLGA nanoparticles plus growth factorsrsquo Biomaterials
34(2) pp 582ndash597 doi 101016jbiomaterials201210001
Penaloza J P et al (2017) lsquoIntracellular trafficking and cellular uptake mechanism of PHBV
nanoparticles for targeted delivery in epithelial cell linesrsquo Journal of nanobiotechnology
15(1) p 1 doi 101186s12951-016-0241-6
Perez C De Jesus P and Griebenow K (2002) lsquoPreservation of lysozyme structure and
function upon encapsulation and release from poly(lactic-co-glycolic) acid microspheres
prepared by the water-in-oil-in-water methodrsquo International journal of pharmaceutics
248(1ndash2) pp 193ndash206 doi 101016s0378-5173(02)00435-0
Peula-Garcia J M Hidaldo-Alvarez R and De las Nieves F J (1997) lsquoProtein co-adsorption
144
on different polystyrene latexes Electrokinetic characterization and colloidal stabilityrsquo
Colloid and Polymer Science 275(2) pp 198ndash202 doi 101007s003960050072
Peula-Garciacutea J M Hidalgo-Alvarez R and De Las Nieves F J (1997) lsquoColloid stability and
electrokinetic characterization of polymer colloids prepared by different methodsrsquo Colloids
and Surfaces A Physicochemical and Engineering Aspects 127(1ndash3) pp 19ndash24 doi
101016S0927-7757(96)03890-3
Peula JM Callejas J de las Nieves F J (1994) lsquoAdsorption of Monomeric Bovine Serum
Albumin on Sulfonated Polystyrene Model Colloids II Electrokinetic Characterization of
Latex-Protein Complexesrsquo Surface Properties of Biomaterials pp 61ndash69
Peula J M Hidalgo-Alvarez R and de las Nieves F J (1995) lsquoCoadsorption of IgG and BSA
onto sulfonated polystyrene latex I Sequential and competitive coadsorption isothermsrsquo
Journal of biomaterials science Polymer edition 7(3) pp 231ndash240 doi
101163156856295x00274
Peula J M and de las Nieves F J (1993) lsquoAdsorption of monomeric bovine serum albumin
on sulfonated polystyrene model colloids 1 Adsorption isotherms and effect of the surface
charge densityrsquo Colloids and Surfaces A Physicochemical and Engineering Aspects 77(3) pp
199ndash208 doi 1010160927-7757(93)80117-W
Peula J M and de las Nieves F J (1994) lsquoAdsorption of monomeric bovine serum albumin
on sulfonated polystyrene model colloids 3 Colloidal stability of latex-protein complexesrsquo
Colloids and Surfaces A Physicochemical and Engineering Aspects 90(1) pp 55ndash62 doi
1010160927-7757(94)02889-3
Pezennec S et al (2008) The protein net electric charge determines the surface rheological
properties of ovalbumin adsorbed at the air-water interface Available at
wwwelseviercomlocatefoodhyd (Accessed 29 March 2020)
Pirooznia N et al (2012) lsquoEncapsulation of alpha-1 antitrypsin in PLGA nanoparticles in
145
vitro characterization as an effective aerosol formulation in pulmonary diseasesrsquo Journal of
nanobiotechnology 10 p 20 doi 1011861477-3155-10-20
Poinern G E J (no date) A laboratory course in nanoscience and nanotechnology
Puppi D et al (2014) lsquoNanomicrofibrous polymeric constructs loaded with bioactive
agents and designed for tissue engineering applications a reviewrsquo Journal of biomedical
materials research Part B Applied biomaterials 102(7) pp 1562ndash1579 doi
101002jbmb33144
Qutachi O Shakesheff K M and Buttery L D K (2013) lsquoDelivery of definable number of
drug or growth factor loaded poly(DL-lactic acid-co-glycolic acid) microparticles within
human embryonic stem cell derived aggregatesrsquo Journal of controlled release official
journal of the Controlled Release Society 168(1) pp 18ndash27 doi
101016jjconrel201302029
Rafati A et al (2012) lsquoChemical and spatial analysis of protein loaded PLGA microspheres
for drug delivery applicationsrsquo Journal of controlled release official journal of the Controlled
Release Society 162(2) pp 321ndash329 doi 101016jjconrel201205008
Rahman C V et al (2014) lsquoControlled release of BMP-2 from a sintered polymer scaffold
enhances bone repair in a mouse calvarial defect modelrsquo Journal of tissue engineering and
regenerative medicine 8(1) pp 59ndash66 doi 101002term1497
Ramel M-C and Hill C S (2012) lsquoSpatial regulation of BMP activityrsquo FEBS letters 586(14)
pp 1929ndash1941 doi 101016jfebslet201202035
Ratzinger G et al (2010) lsquoSurface modification of PLGA particles the interplay between
stabilizer ligand size and hydrophobic interactionsrsquo Langmuir the ACS journal of surfaces
and colloids 26(3) pp 1855ndash1859 doi 101021la902602z
Rescignano N et al (2016) lsquoIn-vitro degradation of PLGA nanoparticles in aqueous medium
and in stem cell cultures by monitoring the cargo fluorescence spectrumrsquo Polymer
146
Degradation and Stability 134 pp 296ndash304 doi 101016jpolymdegradstab201610017
van Rijt S and Habibovic P (2017) lsquoEnhancing regenerative approaches with
nanoparticlesrsquo Journal of the Royal Society Interface 14(129) doi 101098rsif20170093
Romagnoli C DrsquoAsta F and Brandi M L (2013) lsquoDrug delivery using composite scaffolds
in the context of bone tissue engineeringrsquo Clinical cases in mineral and bone metabolism
the official journal of the Italian Society of Osteoporosis Mineral Metabolism and Skeletal
Diseases 10(3) pp 155ndash161
Ronga M et al (2013) lsquoClinical applications of growth factors in bone injuries experience
with BMPsrsquo Injury 44 Suppl 1 pp S34-9 doi 101016S0020-1383(13)70008-1
Rosca I D Watari F and Uo M (2004) lsquoMicroparticle formation and its mechanism in
single and double emulsion solvent evaporationrsquo Journal of controlled release official
journal of the Controlled Release Society 99(2) pp 271ndash280 doi
101016jjconrel200407007
Sanchez-Moreno P et al (2013) lsquoSynthesis and characterization of lipid immuno-
nanocapsules for directed drug delivery selective antitumor activity against HER2 positive
breast-cancer cellsrsquo Biomacromolecules 14(12) pp 4248ndash4259 doi 101021bm401103t
Santander-Ortega M J et al (2006) lsquoColloidal stability of pluronic F68-coated PLGA
nanoparticles a variety of stabilisation mechanismsrsquo Journal of colloid and interface science
302(2) pp 522ndash529 doi 101016jjcis200607031
Santander-Ortega M J et al (2009) lsquoInsulin-loaded PLGA nanoparticles for oral
administration an in vitro physico-chemical characterizationrsquo Journal of biomedical
nanotechnology 5(1) pp 45ndash53 doi 101166jbn2009022
Santander-Ortega M J et al (2010) lsquoNanoparticles made from novel starch derivatives for
transdermal drug deliveryrsquo Journal of controlled release official journal of the Controlled
Release Society 141(1) pp 85ndash92 doi 101016jjconrel200908012
147
Santander-Ortega Manuel J Lozano-Loacutepez M V et al (2010) lsquoNovel core-shell lipid-
chitosan and lipid-poloxamer nanocapsules Stability by hydration forcesrsquo Colloid and
Polymer Science 288(2) pp 159ndash172 doi 101007s00396-009-2132-y
Santander-Ortega Manuel J Csaba N et al (2010) lsquoProtein-loaded PLGA-PEO blend
nanoparticles Encapsulation release and degradation characteristicsrsquo Colloid and Polymer
Science 288(2) pp 141ndash150 doi 101007s00396-009-2131-z
Santander-Ortega M J et al (2011) lsquoChitosan nanocapsules Effect of chitosan molecular
weight and acetylation degree on electrokinetic behaviour and colloidal stabilityrsquo Colloids
and surfaces B Biointerfaces 82(2) pp 571ndash580 doi 101016jcolsurfb201010019
Santander-Ortega M J Bastos-Gonzalez D and Ortega-Vinuesa J L (2007)
lsquoElectrophoretic mobility and colloidal stability of PLGA particles coated with IgGrsquo Colloids
and surfaces B Biointerfaces 60(1) pp 80ndash88 doi 101016jcolsurfb200706002
Santo V E et al (2012) lsquoFrom nano- to macro-scale nanotechnology approaches for
spatially controlled delivery of bioactive factors for bone and cartilage engineeringrsquo
Nanomedicine (London England) 7(7) pp 1045ndash1066 doi 102217nnm1278
Sapkota G et al (2007) lsquoBalancing BMP signaling through integrated inputs into the Smad1
linkerrsquo Molecular cell 25(3) pp 441ndash454 doi 101016jmolcel200701006
Schrier J A et al (2001) lsquoEffect of a freeze-dried CMCPLGA microsphere matrix of rhBMP-
2 on bone healingrsquo AAPS PharmSciTech 2(3) p E18 doi 101208pt020318
Schrier J A and DeLuca P P (2001) lsquoPorous bone morphogenetic protein-2 microspheres
polymer binding and in vitro releasersquo AAPS PharmSciTech 2(3) p E17 doi
101208pt020317
Schwendeman S P et al (2014) lsquoInjectable controlled release depots for large moleculesrsquo
Journal of controlled release official journal of the Controlled Release Society 190 pp 240ndash
253 doi 101016jjconrel201405057
148
Shankarayan R Kumar S and Mishra P (2013) lsquoDifferential permeation of piroxicam-
loaded PLGA micronanoparticles and their in vitro enhancementrsquo Journal of Nanoparticle
Research 15(3) doi 101007s11051-013-1496-6
Shim Y B et al (2016) lsquoFabrication of hollow porous PLGA microspheres using sucrose for
controlled dual delivery of dexamethasone and BMP2rsquo Journal of Industrial and Engineering
Chemistry 37 pp 101ndash106 doi 101016jjiec201603014
Siafaka P I et al (2016) lsquoSurface Modified Multifunctional and Stimuli Responsive
Nanoparticles for Drug Targeting Current Status and Usesrsquo International journal of
molecular sciences 17(9) doi 103390ijms17091440
Sieber C et al (2009) lsquoRecent advances in BMP receptor signalingrsquo Cytokine amp growth factor
reviews 20(5ndash6) pp 343ndash355 doi 101016jcytogfr200910007
Silva G A et al (2007) lsquoMaterials in particulate form for tissue engineering 2 Applications
in bonersquo Journal of tissue engineering and regenerative medicine 1(2) pp 97ndash109 doi
101002term1
Simmonds M C et al (2013) lsquoSafety and effectiveness of recombinant human bone
morphogenetic protein-2 for spinal fusion a meta-analysis of individual-participant datarsquo
Annals of internal medicine 158(12) pp 877ndash889 doi 1073260003-4819-158-12-
201306180-00005
Sneh-Edri H Likhtenshtein D and Stepensky D (2011) lsquoIntracellular targeting of PLGA
nanoparticles encapsulating antigenic peptide to the endoplasmic reticulum of dendritic
cells and its effect on antigen cross-presentation in vitrorsquo Molecular pharmaceutics 8(4)
pp 1266ndash1275 doi 101021mp200198c
Spagnoli D B and Marx R E (2011) lsquoDental implants and the use of rhBMP-2rsquo Dental clinics
of North America 55(4) pp 883ndash907 doi 101016jcden201107014
Srinivasan C et al (2005) lsquoEffect of additives on encapsulation efficiency stability and
149
bioactivity of entrapped lysozyme from biodegradable polymer particlesrsquo Journal of
microencapsulation 22(2) pp 127ndash138 doi 10108002652040400026400
Sturesson C and Carlfors J (2000) lsquoIncorporation of protein in PLG-microspheres with
retention of bioactivityrsquo Journal of controlled release official journal of the Controlled
Release Society 67(2ndash3) pp 171ndash178 doi 101016s0168-3659(00)00205-4
Sun D (2016) lsquoEffect of Zeta Potential and Particle Size on the Stability of SiO2 Nanospheres
as Carrier for Ultrasound Imaging Contrast Agentsrsquo Int J Electrochem Sci pp 8520ndash8529
Tan J S et al (1993) lsquoSurface modification of nanoparticles by PEOPPO block copolymers
to minimize interactions with blood components and prolong blood circulation in ratsrsquo
Biomaterials 14(11) pp 823ndash833 doi 1010160142-9612(93)90004-l
Tian Z et al (2012) lsquoSynthesis and characterization of UPPE-PLGA-rhBMP2 scaffolds for
bone regenerationrsquo Journal of Huazhong University of Science and Technology Medical
sciences = Hua zhong ke ji da xue xue bao Yi xue Ying De wen ban = Huazhong keji daxue
xuebao Yixue Yingdewen ban 32(4) pp 563ndash570 doi 101007s11596-012-0097-4
Torcello-Goacutemez A et al (2011) lsquoAdsorption of antibody onto Pluronic F68-covered
nanoparticles Link with surface propertiesrsquo Soft Matter 7(18) pp 8450ndash8461 doi
101039c1sm05570d
Torrecillas-Martinez L et al (2013) lsquoEffect of rhBMP-2 upon maxillary sinus augmentation
a comprehensive reviewrsquo Implant dentistry 22(3) pp 232ndash237 doi
101097ID0b013e31829262a8
Tran M-K Swed A and Boury F (2012) lsquoPreparation of polymeric particles in CO(2)
medium using non-toxic solvents formulation and comparisons with a phase separation
methodrsquo European journal of pharmaceutics and biopharmaceutics official journal of
Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik eV 82(3) pp 498ndash507 doi
101016jejpb201208005
150
Tsuji K et al (2006) lsquoBMP2 activity although dispensable for bone formation is required
for the initiation of fracture healingrsquo Nature genetics 38(12) pp 1424ndash1429 doi
101038ng1916
Tsuji K et al (2008) lsquoBMP4 is dispensable for skeletogenesis and fracture-healing in the
limbrsquo The Journal of bone and joint surgery American volume 90 Suppl 1 pp 14ndash18 doi
102106JBJSG01109
Tsuji K et al (2010) lsquoConditional deletion of BMP7 from the limb skeleton does not affect
bone formation or fracture repairrsquo Journal of orthopaedic research official publication of
the Orthopaedic Research Society 28(3) pp 384ndash389 doi 101002jor20996
Urist M R (1965) lsquoBone formation by autoinductionrsquo Science (New York NY) 150(3698)
pp 893ndash899 doi 101126science1503698893
Vasir J K and Labhasetwar V (2007) lsquoBiodegradable nanoparticles for cytosolic delivery
of therapeuticsrsquo Advanced drug delivery reviews 59(8) pp 718ndash728 doi
101016jaddr200706003
Vo T N Kasper F K and Mikos A G (2012) lsquoStrategies for controlled delivery of growth
factors and cells for bone regenerationrsquo Advanced drug delivery reviews 64(12) pp 1292ndash
1309 doi 101016jaddr201201016
Wallace S S and Froum S J (2003) lsquoEffect of maxillary sinus augmentation on the survival
of endosseous dental implants A systematic reviewrsquo Annals of periodontology 8(1) pp
328ndash343 doi 101902annals200381328
Wan F and Yang M (2016) lsquoDesign of PLGA-based depot delivery systems for
biopharmaceuticals prepared by spray dryingrsquo International journal of pharmaceutics
498(1ndash2) pp 82ndash95 doi 101016jijpharm201512025
Wang E A et al (1990) lsquoRecombinant human bone morphogenetic protein induces bone
formationrsquo Proceedings of the National Academy of Sciences of the United States of America
151
87(6) pp 2220ndash2224 doi 101073pnas8762220
Wang H et al (2012) lsquoThe use of micro- and nanospheres as functional components for
bone tissue regenerationrsquo Tissue engineering Part B Reviews 18(1) pp 24ndash39 doi
101089tenTEB20110184
Wang Y et al (2015) lsquoPLGAPDLLA core-shell submicron spheres sequential release
system Preparation characterization and promotion of bone regeneration in vitro and in
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Wege H A Holgado-Terriza J A and Cabrerizo-Vilchez M A (2002) lsquoDevelopment of a
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101006jcis20028233
Wheeler S L (1997) lsquoSinus augmentation for dental implants the use of alloplastic
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2391(97)90186-5
White L J et al (2013) lsquoAccelerating protein release from microparticles for regenerative
medicine applicationsrsquo Materials science amp engineering C Materials for biological
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Wozney J M (1992) lsquoThe bone morphogenetic protein family and osteogenesisrsquo Molecular
reproduction and development 32(2) pp 160ndash167 doi 101002mrd1080320212
Xia Y et al (2013) lsquoProtein encapsulation in and release from monodisperse double-wall
polymer microspheresrsquo Journal of pharmaceutical sciences 102(5) pp 1601ndash1609 doi
101002jps23511
Xiong S et al (2011) lsquoCellular uptake of Poly-(DL-lactide-co-glycolide) (PLGA)
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152
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Xu Y et al (2017) lsquoPolymer degradation and drug delivery in PLGA-based drug-polymer
applications A review of experiments and theoriesrsquo Journal of biomedical materials
research Part B Applied biomaterials 105(6) pp 1692ndash1716 doi 101002jbmb33648
Yallapu M M et al (2010) lsquoFabrication of curcumin encapsulated PLGA nanoparticles for
improved therapeutic effects in metastatic cancer cellsrsquo Journal of colloid and interface
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Yameen B et al (2014) lsquoInsight into nanoparticle cellular uptake and intracellular
targetingrsquo Journal of controlled release official journal of the Controlled Release Society 190
pp 485ndash499 doi 101016jjconrel201406038
Yang Y Y Chia H H and Chung T S (2000) lsquoEffect of preparation temperature on the
characteristics and release profiles of PLGA microspheres containing protein fabricated by
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3659(00)00291-1
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doi 101016jbiomaterials200903024
Yilgor P Hasirci N and Hasirci V (2010) lsquoSequential BMP-2BMP-7 delivery from
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536 doi 101002jbma32520
153
Zhang H-X et al (2016) lsquoIn vitro and in vivo evaluation of calcium phosphate composite
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154
10ANEXO MATERIAL SUPLEMENTARIO
httprsbwebnihgovij
155
156
11ANEXO DE PUBLICACIONES
Review ArticleBone Regeneration from PLGA Micro-Nanoparticles
Inmaculada Ortega-Oller1 Miguel Padial-Molina1 Pablo Galindo-Moreno1
Francisco OrsquoValle2 Ana Beleacuten Joacutedar-Reyes3 and Jose Manuel Peula-Garciacutea34
1Department of Oral Surgery and Implant Dentistry University of Granada 18011 Granada Spain2Department of Pathology School of Medicine and IBIMER University of Granada 18012 Granada Spain3Biocolloid and Fluid Physics Group Department of Applied Physics University of Granada 18071 Granada Spain4Department of Applied Physics II University of Malaga 29071 Malaga Spain
Correspondence should be addressed to Jose Manuel Peula-Garcıa jmpeulaumaes
Received 27 March 2015 Accepted 4 June 2015
Academic Editor Hojae Bae
Copyright copy 2015 Inmaculada Ortega-Oller et al This is an open access article distributed under the Creative CommonsAttribution License which permits unrestricted use distribution and reproduction in any medium provided the original work isproperly cited
Poly-lactic-co-glycolic acid (PLGA) is one of the most widely used synthetic polymers for development of delivery systems fordrugs and therapeutic biomolecules and as component of tissue engineering applications Its properties and versatility allow it tobe a reference polymer in manufacturing of nano- and microparticles to encapsulate and deliver a wide variety of hydrophobic andhydrophilic molecules It additionally facilitates and extends its use to encapsulate biomolecules such as proteins or nucleic acidsthat can be released in a controlled wayThis review focuses on the use of nanomicroparticles of PLGA as a delivery system of oneof the most commonly used growth factors in bone tissue engineering the bone morphogenetic protein 2 (BMP2) Thus all theneeded requirements to reach a controlled delivery of BMP2 using PLGA particles as a main component have been examinedTheproblems and solutions for the adequate development of this system with a great potential in cell differentiation and proliferationprocesses under a bone regenerative point of view are discussed
1 Introduction
Bone regeneration is one of the main challenges facing us inthe daily clinic Immediately after a tooth extraction normalbiological processes remodel the alveolar bone limiting insome cases the possibility of future implant placementDifferent strategies for the preservation of that bone havebeen explored in recent years Other conditions such astrauma tumor resective surgery or congenital deformitiesrequire even higher technical and biological requirementsto generate the necessary bony structure for the occlusalrehabilitation of the patient To overcome these anatomicallimitations in terms of bone volume different approacheshave been proposed to either improve the implant osteoin-tegration or to augment the bone anatomy where it will beplaced [1 2] Autogenous bone graft is still considered theldquogold standardrdquo due to its osteogenic osteoconductive andosteoconductive properties [3 4] However it also presentsseveral limitations including the need for a second surgery
limited availability and morbidity in the donor area [5]Therefore other biomaterials such as allogeneic grafts withosteoconductivity and osteoinductive capacities [6 7] andxenogeneic grafts [8 9] and alloplastic biomaterials [10]with osseoconductive potential were proposed All thesematerials although acceptable are not suitable in manyconditions and usually require additional consideration inthe decision process [11] Additionally the bone quantity andquality that can be obtained with these materials are oftenlimited
The use of bioactive molecules alone or in combina-tion with the previously described materials has thereforebecome amajor area of interest thanks to their high potentialWhen using this kind of procedures it is important toconsider (1) the delivery method and (2) the molecule itselfBioactive molecules can be transported into the defect areaas a solution or a gel embedded in sponges adhered to solidscaffolds and more recently included in particles of differentsizes Using these methods PDGF (platelet-derived growth
Hindawi Publishing CorporationBioMed Research InternationalVolume 2015 Article ID 415289 18 pageshttpdxdoiorg1011552015415289
2 BioMed Research International
factor) FGF (fibroblast growth factor) IGF (insulin growthfactor) Runx2 Osterix (Osx) LIM domain mineralizationprotein (LMP) BMP (bone morphogenic protein) and morerecently periostin have been proposed as potential candidatesfor regeneration procedures within the oral cavity includingbone and periodontal tissues [12 13] These molecules havebeen tested alone or in combination with stem cells [14] usingseveral in vitro and in vivo strategies [15]
Consequently within the context of this review we intendto review the delivery methods of bioactive molecules withthe purpose of bone regeneration with a particular focus onpolymeric nanomicroparticles especially those with PLGAas main component to encapsulate the growth factor BMP-2 An overview of the biological functions of bone morpho-genetic proteins and an analysis of the different parametersaffecting the physicochemical properties of these systems arepresented Synthesis method particle size and morphologyuse of stabilizers and their incidence in the colloidal sta-bility protective function and surface functionality will bediscussed In addition we explore the different strategies thatcan be used to optimize the encapsulation efficiency andrelease kinetics main parameters that determine the correctdevelopment of polymeric carriers used in tissue-engineeredbone processes
2 BMPs Action and Regulation
For bone regeneration in particular bone morphogeneticgrowth factors (BMP) are probably the more tested groupof molecules Since 1965 when Urist [16] showed that theextracted bone BMPs could induce bone and cartilage forma-tion when implanted in animal tissue an increasing numberof reports have tested its in vivo application and biologicalfoundation when used in bone defects [17ndash19] BMPs aremembers of the TGF-120573 superfamily of proteins [20] TheBMP family of proteins groups more than 20 homodimericor heterodimericmorphogenetic proteins which functions inmany cell types and tissues not all of them being osteogenic[21] BMPs can be divided into 4 subfamilies based on theirfunction and sequence being BMP-2 BMP-4 and BMP-7 the ones with osteogenic potential [21] The actions ofBMPs include chondrogenesis osteogenesis angiogenesisand extracellular matrix synthesis [22] Within this fam-ily of proteins BMP-2 has been the most studied It hasosteoinductive properties that promote the formation of newbone by initiating stimulating and amplifying the cascadeof bone formation through chemotaxis and stimulationof proliferation and differentiation of the osteoblastic celllineage [5 17 19 20]The absence of it as studied in knockoutmodels leads to spontaneous fractures that do not heal withtime [23] In fact other models have demonstrated that theabsence of either BMP-4 [24] or BMP-7 [25] do not lead tobone formation and function impairmentwhich demonstratethe compensatory effect produced by BMP-2 alone [26]
Many cell types in bone tissue produce BMPs includingosteoprogenitor cells osteoblasts chrondrocytes plateletsand endothelial cells This secreted BMP is then stored in theextracellular matrix where it mostly interacts with collagentype IV [27] During the repair and remodeling processes
BMPs
BMPR-I BMPR-II
Smad 1 Smad 5
Smad 8Smad 4
Runx2 Dlx5 Osterix
Osteogenesis
Bone resorption
Figure 1 Schematic representation of the main BMP molecularpathway to osteogenesis BMPs interact with cell surface receptorsI and II to activate Smads 1 5 and 8 These activated Smads activateSmad 4 All together as a protein complex activate Runx2 Dlx5 andOsterix
osteoclast resorptive activity induces the release of BMPs tothe medium so that they are suspended and can interact withnearby cells to initiate the subsequent osteogenic process [28]
A BMP in the extracellular matrix binds to cell surfacereceptors BMPR-I and BMPR-II and activates the Smadcytoplasmic proteins or the MAPK pathway [29] WhenBMPR-I is activated BMPR-II is recruited and activatedas well [30] The activation of the complexes BMPR-I andBMPR-II leads to the activation of several Smads (1 5 and 8)that also activate Smad 4 and they all form protein complexesthat are transported into the nucleus where Runx2 Dlx5and Osterix genes (important in osteogenesis) are activated[26 27] (Figure 1) Similarly when the MAPK pathwayis activated it leads to induction of Runx2 transcriptionand therefore to bone differentiation [31] A number ofextracellular and intracellular antagonists have also beendescribed including noggin chordin and gremlin or Smads6 7 and 8b respectively [32]
21 Clinical Use of BMP-2 Today the BMP-2 is commerciallyavailable under different brand names and concentrations Itusually consists of a collagen absorbable sponge embeddedwith recombinant human BMP-2 In 2002 it was approvedby the FDA as an alternative of autogenous bone graftingin anterior lumbar interbody fusion [33] Later in 2007 theFDA approved the use of rhBMP-2 as an alternative forautogenous bone grafting in the increase of the alveolar crestdefects associated with the tooth extraction maxillary sinuspneumatization [33]
Beside the applications in spine clinical studies wherevery high concentrations are used (AMPLIFY rhBMP-240mg) clinical studies have supported its use in the oral
BioMed Research International 3
cavity BMPs have been used in periodontal regenerationbone healing implant osteointegration oral surgery withorthodontic purposes bone pathology sequel repair distrac-tion osteogenesis and endodontic reparative surgery [28 34]However it has shownmore promising results in cases whereonly bone tissue is to be regenerated including preimplantsite development sinus lift vertical and horizontal ridgeaugmentation and dental implant wound healing [35] In thissense it has been shown that the use of rhBMP-2 induced theformation of bone suitable for placement of dental implantsand their osteointegration [36] Furthermore it appears thatthe newly formed bone has similar properties to the nativebone and is therefore capable of supporting denture occlusalforces [37] In the particular case of sinus lifting where bonedeficiency is greater and therefore supportive therapies canbe more helpful a recent meta-analysis found a total of 3human studies and 4 animal trials (Table 1) [38] In summarythe included studies concluded that rhBMP-2 induces newbone formation with comparable bone quality and quantityof newly formed bone to that induced by autogenous bonegraft In some cases even higher bone quality and quantityhave been reported [39]
Conversely recent studies report severe complicationsafter its use [61] Even more high doses have also associatedwith carcinogenic effects which led the authors to emphasizethe need for better guidelines in BMP clinical use [62] Notso drastic recent studies are highlighting the negative sideeffects and risks of its application making high emphasison potential bias of nonreproducible industry sponsoredresearch especially when used in spinal fusion [44 63 64]The use of rhBMP-2 has been shown to increase the risks forwound complications and dysphagia with high effectivenessand harms misrepresentation through selective reportingduplicate publication and underreporting [44] Specificallyin oral bone regenerative applications a report in sinus liftconcluded that the use of BMP-2 promotes negative effectson bone formation when combined with anorganic bovinebone matrix versus anorganic bovine bone alone [41] incontrast with previous reports and reviews [38] Takingtogether this information it can be concluded that it is ofextreme importance to be careful with the clinical use of newproducts avoiding off-label applications It is also importantto highlight the need for more and better clinical research
To overcome these limitations new strategies such asthe use of ex vivo BMP-2-engineered autologous MSCs [65]encapsulation of the protein in different biomaterials ordelivery by gene therapy are being explored in recent years
The development of these technologies is based on somebiological facts In vitro effects of BMPs are observed at verylow dosages (5ndash20 ngmL) although current commerciallyavailable rhBMPs are used in large dosages (up to 40mg ofsome products) [28] This is probably due to an intense pro-teolytic consumption during the early postsurgical phases Itis important to know the proper sequence of biological eventsthat lead to normal tissue healing Then this knowledge canbe used to intervene at the specific time frame where ourtherapy is intended to act [15] Effective bone formation asdescribed above is a sequential processTherefore the induc-tive agent should be delivered at a maintained concentration
during a timeframe In this sense as in many other processesin medicine it has been recently demonstrated that long-term release of BMP-2 is more effective than short-term overa range of doses [51] It is also important to note that therole of other molecular pathways and crosstalk between thedifferent components playing in bone regeneration is notperfectly understood yet and therefore more research hasto be conducted
What is known so far in summary is that BMPs specif-ically BMP-2 is of utility for promoting bone regeneration[28] However the currently FDA-approved BMP-2 deliverysystem (INFUSE Medtronic Sofamor Danek Inc) presentsimportant limitations [66] Firstly protein is quickly inacti-vatedTherefore its biological action disappears maybe evenbefore the blood clot that forms after the surgery is beingorganized Second the recombinant protein is delivered inan absorbable collagen sponge Thus the distribution ofthe BMP in a liquid suspension embedded into a collagensponge makes it impossible to be certain that the protein isreaching the ideal target Therefore where when and forhow long a dose of BMP-2 is reached (determined by thedelivery method) are important factors Because of that newforms of BMP-2 delivery are being developed These newtechnologies have to guarantee a higher half-life of the proteinand a stepped release to increase the effects on the desiredcell targets The biotechnology opens the door to be able toprovide a solution to these limitations
Biodegradable nanoparticles (nanospheres and nanocap-sules) have developed as a promising important tool forthe delivery of macromolecules via parenteral mucous andtopical applications [67ndash70] Well-established biodegradablepolymers such as poly(acid D L-lactic) or poly(D L-lactic-co-glycolic) have been widely used in the preparation ofnanoparticles in recent decades because of its biocompati-bility and full biodegradability [71] However it is knownthat certain macromolecules such as proteins or peptidesmay lose activity during their encapsulation storage deliveryand release [72] To overcome this problem the addition ofstabilizers such as oxide polyethylene (PEO) or the coencap-sulation with other macromolecules and its derivatives seemto be a promising strategy
3 Polymeric Colloidal Particles to EncapsulateHydrophilic Molecules
Generally polymeric colloidal particles are hard systemswith a homogeneous spherical shape composed by naturalor synthetic polymers In order to encapsulate hydrophilicmolecules as proteins or nucleic acids it is necessary to opti-mize the polymeric composition and the synthesis methodIn this process a high encapsulation efficiency maintenanceof the biological activity of the encapsulated biomolecule andobtaining of an adequate release pattern have to be achieved[73ndash75] Several delivery systems of BMP2 (and other growthfactors GFs) using polymeric particles have been describedin the literature Most of them are microparticulated systemsusing the biocompatible and biodegradable PLGA copoly-mer as main component [76 77] Taking into account theincorporation of BMP2 to the carrier system encapsulation
4 BioMed Research InternationalTa
ble1
Sum
maryo
fclin
icalandanim
alstu
diesusingB
MP-2for
sinus
floor
elevatio
n(adapted
from[38])Th
eincludedstu
diesoverallcon
cludedthatrhBM
P-2ind
ucesnewbo
neform
ation
with
comparableb
oneq
ualityandqu
antityof
newlyform
edbo
neto
thatindu
cedby
autogeno
usbo
negraft
Reference
Stud
ydesig
nFo
llow-up
(mon
ths)
Species
(sub
jects)
Coreb
iopsy
harvestin
g(m
onths)
Graftmaterial
New
lyform
edbo
neBo
neheight
gain
(mm)
Bone
width
gain
(mm)
Bone
density
(mgmL)
Immun
erespon
seHistolog
y
Boyn
eetal
2005
[37]
RCT
52Hum
an(48)
6ndash11
075
mgmL
rhBM
P-2AC
S
NA
1129
Crest202
Midpo
int854
Apical118
684
Non
eNA
150m
gmL
rhBM
P-2AC
S047
Crest19
8Midpo
int78
0Ap
ical1078
134
Autogeno
usbo
negraft
autogenou
sbo
negraft
+allogeneicbo
negraft
1016
Crest466
Midpo
int
1017
Apical1056
350
Triplettetal
2009
[40]
RCT
58Hum
an(160)
6
150m
gmL
rhBM
P-2AC
S
NA
783plusmn352
NA
200
Non
e
Rich
vascular
marrowspaceh
ighin
cellu
larc
ontent
Autogeno
usbo
negraft
(iliacc
rest
tibia
ororalcavity)
autogeno
usbo
negraft
+allogeneic
bone
graft
946plusmn411
283
Oste
oclasts
stillpresenthigh
erfib
rous
tissue
Kaoetal2012
[41]
Prospective
6ndash9
Hum
an(22)
6ndash9
rhBM
P-2AC
S+
ABB
1604plusmn74
5
NA
NA
NA
Non
e
Fewer
ABB
particleslessnewlyform
edbo
ne(w
oven
andmatureb
ones
tructure)
ABB
2485plusmn582
MoreA
BBparticlesrem
aining
higher
newlyform
edbo
ne(w
oven
andmatured
bone
structure)
Nevinse
tal
1996
[36]
Prospective
12Goat(6)
12
rhBM
P-2AC
S
NA
NA
NA
NA
Non
e
Dense
isolatedtrabeculae
andbo
nemarrowoste
oblastandosteoclasts
no
corticalbo
ne
ACSBu
ffer
Collageno
usconn
ectiv
etissuen
oevidence
ofinflammation
noneo-osteogenesis
Hanisc
hetal
1997
[42]
RCT
24Non
human
prim
ate(12)
24rhBM
P-2AC
SNA
60plusmn03
NA
144plusmn29
NA
New
lyform
edbo
neindisting
uishable
from
resid
ualbon
eAC
S26plusmn03
139plusmn46
Wadae
tal
2001
[43]
Prospective
8Ra
bbit(10)
8rhBM
P-2AC
S224plusmn44
NA
NA
NA
NA
Cortic
albo
neform
ationin
both
grou
ps
trabeculae
with
clear
lamellarstructure
weree
mbedd
edin
fatty
marrow
Autogeno
usbo
negraft
(iliacc
rest)
219plusmn45
Leee
tal2013
[39]
Prospective
8Mini-p
ig(8)
8rhBM
P-2AC
SNA
93plusmn05
NA
519plusmn3
NA
New
lyform
edcancellous
bonenew
bone
continuo
uswith
resid
entb
onewoven
bone
infib
rovascular
andfatty
marrow
Autogeno
usbo
negraft
(iliacc
rest)
86plusmn07
329plusmn25
Irregu
lara
ndvaria
bleb
onea
mon
gdifferent
subjects
NAnot
availableRC
Trand
omized
clinicaltrialAC
Sabsorbablecollagenspon
geA
BBano
rganicbo
vine
bone
BioMed Research International 5
Hydrophilic biomolecules stabilizers
Organicphase
PLGA and stabilizers(surfactant other polymers) in
DCM acetone or EtAc
Mixture underagitationsonication
Ethanol water surfactants
Antibody
Core
PLGA BSABMP-2
Surface
Surfactant
MicronanosphereMicronanocapsule
ImmunoparticleDirected delivery
Organic solventextraction under
vacuumAqueous phase w1
First w1oemulsion
emulsion
Second polarphase w2
Final w1ow2
120
80
40
150mL
120
80
40
150mL
Figure 2 Double emulsion procedure (wateroilwater emulsion W1OW
2) to obtain PLGA micronanoparticles Depending on the
synthesis conditions (stabilizers solvents and mixing procedure) it is possible to obtain micro-nanospheres with a uniformmatrix or micro-nanocapsules with a core-shell structure Immunoparticles used for directed delivery can be obtained by attaching specific antibodymoleculeson the particle surface
is preferred to absorption because the growth factors aremore protected against environmental factors in the mediumand may have better control over the delivery and release toachieve the desired concentrations in specific site and time[78]
Normally if the GFs are related with bone regenerationprocesses nano-microparticles are trapped in a second sys-tem as hydrogels or tissue engineering scaffolds which alsoplay an important role in the release profile of GFs fromthese particles [78] The nano-microparticles have allowedthe development of multiscale scaffold thereby facilitatingcontrol of the internal architecture and adequate patterns ofmechanical gradients of cells and signaling factors [79]
All steps from the synthesis method and its characteris-tics the encapsulation process or the final surface modifica-tion for a targeted delivery determine the characteristics ofthese systems and their main goal the controlled release ofbioactive GFs
31 Synthesis Methods It is possible to found several pro-cedures to encapsulate hydrophilic molecules as proteinsor nucleic acids in polymeric nanomicroparticles Phase
separation [80] or spray drying [81] techniques have beenreported to encapsulate hydrophilic molecules However inthe case of proteins the most normally used procedureto encapsulate them into PLGA micro- and nanoparticlesis the double-emulsion (wateroilwater WOW) solventevaporation technique [75 82] A schematic description ofthis technique is presented in Figure 2 In a general wayPLGA is dissolved in an organic solvent and emulsified usingmechanical agitation or sonication with water containingan appropriate amount of protein Thus a primary wateroil(WO) emulsion is obtained In the second phase thisemulsion is poured into a large polar phase leading to animmediate precipitation of the particles as a consequenceof the polymer shrinkage around droplets of the primaryemulsion This phase may be composed of a water solutionof a stabilizer (surfactant) or ethanol-water mixtures [8384] After stirring the organic solvent is rapidly extractedby evaporation under vacuum A wide list of differentmodifications have been tested in this procedure in order toobtain a micronanocarrier system with adequate colloidalstability high encapsulation efficiency adequate bioactivityand finally a long-time release profilewith low ldquoinitial burstrdquo
6 BioMed Research International
The goal is to avoid a high amount of protein (gt60) beingreleased very quickly (24 hours) which is one of the biggestproblems of a controlled release system [76]
32 Organic Solvent Hans and Lowman show differentexamples of organic solvents used in multiple emulsionprocesses Normally dichloromethane (DMC) ethyl acetateacetone and their mixtures can be used [82] In the first stepa good organic solvent with low water solubility to facilitatethe emulsification process and low boiling point for an easyevaporation would be the election However the structureof the encapsulated protein molecules can be affected anddenaturation processes and loss of biological activity appearwhen they interact with a typical organic solvent as DMC[73] Ethyl acetate on the other hand exerts less denaturatingeffects with a lower incidence on the bioactivity of theencapsulated proteins [85]
Other important factors related with the organic solventare their physical properties that affect how the polymertails self-organize in the shell of the emulsion droplets andmodify the nanoparticle morphology and the encapsulationefficiency [86] In this way a higher water solubility ofthe organic solvent that is ethyl acetate favors a rapidsolvent removal Additionally the solvent removal rate canbe controlled by adjusting the volume of the polar phase aswell as the shear stress during the second emulsification stepAn increase of these two parameters increases the diffusionrate of ethyl acetate from primary microparticles to outeraqueous phase resulting in their rapid solidification [87] Italso enhances the encapsulation efficiency andminimizes thecontact-time between protein molecules and organic solvent[88] obtaining at the same time a lower burst effect and aslower drug release from the microparticles [87]
33 Particle Size and Morphology Particle size is an impor-tant parameter and one of the main goals of the deliverypolymeric system Microspheres from a few micrometers upto 100 120583m are suitable for oral delivery mucosal adhesion orinside scaffold use that is for bone regeneration Nanoscaledimension of the carrier offers enhanced versatility whencompared with particles of larger size This is due to thefact that they have higher colloidal stability improved dis-persibility and bioavailability more reactive surface and alsocan deliver proteins or drugs inside and outside of thecorresponding cells [89] BMP2 promotes bone formationand induces the expression of other BMPs and initiates thesignaling pathway from the cell surface by binding to twodifferent surface receptors [22] Therefore the BMP2 carrierparticles must release it into the extracellular medium Sincecellular intake of PLGAnanoparticles is very fast the intakingprocess can be limited by an increase in size from nano-to microparticles [90] However the interaction betweenparticles and cells is strongly influenced by particle size Ifcell internalization is desired the particle must be comprisedin the submicron scale at an interval between 2 and 500 nm[91] Moreover this size is needed for a rapid distributionafter parenteral administration in order to reach differenttissues through different biological barriers In addition
Figure 3 Scanning electron microscopy (SEM) photography ofPLGA nanoparticles obtained by a double emulsion emulsificationprocedureThis systemwith spherical shape low polydispersity andnanoscopic scale shows the intended properties for an adequatephysiological distribution and cell internalization
the intake by macrophages is minimized with a diameterof nanoparticles under 200 nm and even smaller [82 92]As discussed by Yang et al [93] slight modifications ofthe synthesis procedure can suppose drastic effects on thesize or particle morphology and therefore in the proteinencapsulation efficiency and kinetic release
In double emulsion processes the first emulsificationstep largely determines the particle size while the secondemulsification step characterized by the solvent eliminationand polymer precipitation mainly affects the particle mor-phology [86] However the use of surfactant solutions asthe polar medium of the second emulsification process andthe volume ratio between organic and polar phases in thisstep has shown an important influence in the final size [94]Therefore the correct election of the organic solvent thepolymer concentration the addition of surfactant and theemulsification energy allow controlling the size of the system
The incorporation of poloxamers (F68) in the organicsolvent of the primary emulsification helps to increase thecolloidal stability of the first dispersion by being placedat the wateroil interface This reduces the particle size incomparison with pure PLGA nanoparticles in which theonly stability source comes from electric charge of thecarboxyl groups of the PLGA [95] It is normal to obtainspherical micronanospheres with a polymeric porous coreA typical SEM micrograph of PLGA nanoparticles obtainedby WOW emulsion using a mixture of organic solvents(DCMacetone) and ethanolwater as second polar mediumis shown in Figure 3 in which the spherical shape anduniform size distribution are the main characteristics Theouter polymeric shell in the second emulsification steppushed the water droplets to the inner core according to theirsolidification process [96] This process allows producingparticles like capsules with a core-shell structure in whichthe inner core has a low polymer density Figure 4 shows atypical core-shell structure in which the polymer precipitatesand shrinks around the water droplets during the solventchange of the second phase and the subsequent organicsolvent evaporation process [97] In this case the process of
BioMed Research International 7
(a) (b)
Figure 4 PLGApoloxamers188 blend nanoparticles (a) Scanning transmission electron microscopy (STEM) photography (b) scanningelectron microscopy (SEM) photography STEM technique allows the analysis of the nanoparticle structure with an internal region with alow polymer density which is representative of nanocapsules with core-shell structure
solidification of the polymer is influenced and determined bythe miscibility of the organic solvent with the second polarphase and the removal rate
The polymeric shell often presents channels or pores asa consequence of the inner water extrusion due to osmoticforcesThis can reduce the encapsulation efficiency and favorsa fast initial leakage with the unwanted ldquoburst releaserdquo [93]This modification of internal structure of the particles isusually indicated assigning the term ldquonanosphererdquo to thesystem with a core consisting of a homogeneous polymermatrix The bioactive agent is dispersed within them whilethe core-shell structure would be similar to a ldquonanocapsulerdquowhere the biomolecule is preferably in the aqueous cavitysurrounded by the polymeric shell [78] (see Figure 2)
34 Stabilizer Agents
341 Colloidal Stability The double emulsion method nor-mally requires the presence of stabilizers in order to confercolloidal stability during the first emulsification step toprevent the coalescence of the emulsion droplets and latertomaintain the stability of the final nanomicroparticles [98]Polyvinyl alcohol (PVA) and PEO derivate as poloxamers(also named pluronics) have been used inmost cases [83 94]Others include natural surfactants such as phospholipids[99 100] In some cases it is possible to avoid surfactantsif the particles have an electrostatic stability contributionthat is from the uncapped end carboxyl groups of the PLGAmolecules [101]
As it has been previously commented PVA and polox-amers have shown their efficiency in synthetizing both nano-and microparticles affecting not only the stability of thesystems but also their size and morphology Thus a sizereduction effect has been found using PVA in the externalwater phase affecting at the same time the surface porosity
mainly in microsized particles [94] A comparative studybetween this and phospholipids (di-palmitoyl phosphaty-dilcholine DPPC) as stabilizers showed that DPPC couldbe a better emulsifier than PVA to produce nano- andmicroparticles With this method a much lower amount ofstabilizer was needed to obtain a similar size In the samestudy a higher porosity on the particle surface for the PVAemulsified nanospheres was shown [99]
On the other hand the combination of PLGA withpoloxamers has shown positive effects for the nano- andmicrosystems in terms of stability [102] The use of thesesurfactants in the first or second steps of the WOWemulsion procedure leads to different situations Thus ifpoloxamers are blended with PLGA in the organic phaseof the primary emulsification an alteration of the surfaceroughness is obtained However if these are added in theinner water phase an increase of porosity is found [83] Inaddition their inclusion in the polar phase of the secondemulsification step also generates hydrophilic roughnesssurfaces A quantification of this is shown in Figure 5 inwhich the electrophoretic mobility of both PLGA pure andPLGApluronic F68 nanoparticles is measured as a functionof the pH of the medium The observed dependence withthis parameter is a consequence of the weak acid characterof the PLGA carboxyl groups When poloxamer moleculesare present at the interface a systematic reduction ofmobilitywas found as a consequence of the increase in the surfaceroughness The hydrophilic surfactant chains spread outtowards the solvent originating a displacement of the shearplane and the consequent mobility reduction [95 101]
The final PLGA particle size is primarily controlled byelectrostatic forces and is not significantly affected by thepresence or nature of poloxamer stabilizers [101] The recog-nition of the nanocarriers by the mononuclear phagocyticsystem (MPS) can be significantly altered if the surface of
8 BioMed Research International
pH4 5 6 7 8 9
minus6
minus4
minus2
0
2
120583e
(V m
minus1
sminus1)
Figure 5 Electrophoretic mobility versus pH for PLGA nano-particles with different characteristics (998787) PLGA (◼) PLGApolox-amer188 blend and (∙) PLGA covered by Immuno-120574-globulin Thedifferent surface composition affects the electrokinetic behaviourof bare nanoparticles Surface charge values were screened by thepresence of nonionic surfactant as poloxamers or in a higherextension by the presence of antibody molecules attached on thesurface
colloidal particles is modified by using PEO block copoly-mer of the poloxamer molecules The steric barrier givenby these surfactant molecules prevents or minimizes theadsorption of plasma protein and decreases the recognitionby macrophages [103] The size of microspheres is alsounaffected by the coencapsulation of poloxamers The sys-tem containing poloxamer-PLGA blends drive to an innerstructure displaying small holes and cavities in relation withmicrospheres of pure PLGA with a compact matrix-typestructure [83]
Microparticles formulated by poloxamer in the secondpolar medium have completely different surface than thePVA ones almost without pores [94] A comparison betweendifferent poloxamers shows that the hydrophilic-lipophylicbalance (HBL) of the surfactant plays a crucial role determin-ing the surfactant-polymer interactions and controlling theporosity and roughness of the nano-microparticles [83 104]
In a similar manner to surfactants polymer character-istics like the hydrophobicity grade the molecular weightor the hydrolysis degradation rate can strongly influencethe particlemorphologyTherefore the polymer compositionof the particles greatly affects its structure and propertiesThis is why it is usual to use other polymers in order tomodify the behavior and application of the particles Inthis way polyethylene glycol (PEG) of different chain lengthis frequently used to modify the surface characteristicsWith PEG particles are more hydrophilic and with roughersurfaces which affects the MPS action by increasing thecirculating-time and half-life in vivo like the presence of PEOchains [105] Additionally PEG chains also provide colloidalstability via steric stabilization Pegylated-PLGA nano- ormicroparticles can be normally obtained by using in the
synthesis method PLGAPEG di- and triblock copolymers[58 59 75] Natural polymers as chitosan besides modifyingthe hydrophobicity-hydrophilicity ratio of the surface alsoconfer them a mucoadhesive character [106]
342 Encapsulation Efficiency and Bioactivity Furthermorethe use of stabilizers (surfactants or polymers) also influ-ences the encapsulation efficiency and the protein stabilityIn fact for the WOW solvent evaporation process thechlorinated organic solvent used for the first emulsificationcould degrade protein molecules encapsulated in this stepif they come into contact with the organicwater interfacecausing their aggregation or denaturation [107]Thepolymer-protein interaction the shear stress for the emulsificationprocess and the pH reduction derived from PLGA polymerdegradation can also produce the same situation with thesubsequent loss of biological activity of the encapsulatedbiomolecules Different strategies to prevent it have beenused For example an increase of the viscosity around proteinmolecules can help to isolate them from their microenviron-ment [108] In this way viscous products such as starch havebeen used to prevent protein instability [109] These authorscoencapsulate BMP2 with albumin inside starch microparti-cles using other biodegradable polymer poly-120576-caprolactoneinstead of PLGA The BMP2 retained its bioactivity Despitea low encapsulation rate beside an initial burst followedby an uncompleted release the amount of BMP2 neededat the beginning was lower [109] The combination of PEOsurfactants with PLGA (blended in the organic phase) canalso preserve the bioactivity of microencapsulated proteins[110] or nucleic acids [84]
However in most cases the coencapsulation of GFswith other biomolecules was the preferred strategy Therebyserum albumins (SA) have shown the capacity to limit theaggregation-destabilization of several proteins incited by thewaterorganic solvent interface of the primary emulsificationprocess [111 112] White et al encapsulated lysozyme insidePLGA-PEG microparticles In addition to the protectivefunction they also observed an important increase of theentrapment efficiency when human SA was coencapsulatedwith lysozyme and BMP2 [59] drsquoAngelo et al used heparinas stabilizer because it forms a specific complex with severalGFs stabilizes their tridimensional structure and promotestheir bioactivity An encapsulation efficiency of 35 wasincreased to 87 using bovine SA as a second stabilizer toencapsulate two natural proangiogenic growth factors insidePLGA-poloxamer blended nanoparticlesThe in vitro cellularassays showed the preservation of the biological activity ofGFs up to one month [56]
The use of more hydrophilic surfactants (poloxamers)or polymers (PEG) in the inner water phase or blendedwith PLGA in the organic phase of the primary emulsionreduces the interaction of encapsulated proteins with thehydrophobic PLGA matrix This prevents disrupting thestructure of the protein molecules and helps at the sametime to neutralize the acidity generated by the hydrolyticdegradation of the PLGA [113] In some cases the combina-tion of several stabilizers such as poloxamers trehalose andsodium bicarbonate has been shown to preserve the integrity
BioMed Research International 9
of encapsulated proteins but it also reduces the encapsulationefficiency [114]
As a general rule encapsulation efficiency increases withthe size of the particles [82] Additionally the adequate sta-bilization of the primary emulsion by amphiphilic polymersand a rapid solidification (precipitation) of polymer in thesecond step are favorable parameters for enhancing proteinentrapment efficiency in the WOW emulsion technique[87]
The tendency of BMP2 to interact with hydrophobicsurfacesmay decrease the loss of encapsulated protein duringthe extraction of the solvent phase This favors a higherentrapment but it lowers the later extraction [58] An optimalprotein encapsulation is obtained when pH of the internaland external water phases is near the isoelectric point of theprotein [92] Blanco and Alonso [83] observed a reductionin the protein encapsulation efficiency when poloxamer wascoencapsulated in the primary emulsion This highlights themain role played by the protein-polymer interaction in theencapsulation efficiency and the later release process How-ever too much emulsifier may also result in a reduction ofthe encapsulation efficiency [99] Therefore an equilibriumbetween the emulsification powder of the surfactant and theirconcentration is needed
35 Release Profile The release profile represents one of themost important characteristics of a nanomicro particulatecarrier system since their development has a main finalobjective the adequate release of the encapsulated bioactivemolecules to reach the desired clinical action
The release pattern of protein encapsulated in PLGAmicronanoparticles can present different behavior It ispossible to find a continuous release when the diffusionof the biomolecule is faster than the particle erosion Thisprocess involves a continuous diffusion of the protein fromthe polymer matrix before the PLGA particle is degradedin lactic and glycolic acid monomers by hydrolysis [74] Abiphasic release characterized by an initial burst at or nearthe particle surface followed by a second phase in whichprotein is progressively released by diffusion has also beendescribedThe second phase can be enhanced by bulk erosionof PLGA shell and matrix which results in an importantincrease of pores and channels [75] A third triphasic releaseprofile has been found when a lag release period occursafter initial burst and until polymer degradation starts [115]Finally it is possible to obtain an incomplete protein releaseas a consequence of additional factors related with theprotein-polymer interaction or protein instability Figure 6illustrates the different release profiles previously describedThe optimal carrier system should be capable of releasinga controlled concentration gradient of growth factors inthe appropriate time preventing or at least reducing orcontrolling the initial burst effect [116] A controlled initialburst followed by a sustained release significantly improvesthe in vivo bone regeneration [117ndash119]
Giteau et al [108] present an interesting revision on ldquoHowto achieve a sustained and complete release from PLGAmicroparticlesrdquo They begin by analyzing the influence of therelease medium and sampling method on the release profile
00
20
40
60
80
100
Cum
ulat
ive r
eleas
e of p
rote
in (
)
Time (h)500400300200100
Figure 6 Release profiles (I) BSA release from PLGA nanoparti-cles with high initial burst release (red dots line) biphasic modelcombining a moderate initial burst and a subsequent sustainedrelease (blue dash line) triphasicmodel with a lag of release betweenboth initial and sustained release phases (dash-dot green line)incomplete release
and highlight the significance of the centrifugation cleaningprocess or the releasemedium volume Adjusting to adequatevalues the centrifugation speed or the buffer volume itis possible to separate micronanoparticles from protein-containing release medium in a very easy wayThis allows forstable and reproducible release patterns On the other handto ensure a better protein release profile modification of themicroparticle formulation and microencapsulation processin order to preserve protein aggregation has to be performedProtein stability has to be maintained by preventing theformation of harmful medium For example the synthesisformulation can be modified to use more hydrophilic poly-mers since they have been shown to reduce the initial burstand to deliver bioactive proteins over long time periods
The most relevant strategies are referenced below Drugrelease from PLGA nanomicroparticles can be controlledby the polymer molecular weight and the relation betweenmonomers (lactideglycolide) so that an increase in gly-colic acid accelerates the weight loss of polymer due tothe higher hydrophilicity of the matrix [75] A mixtureof different PLGA nanoparticles obtained using 50 50 and75 50 latideglycolide ratio has shown a great potential forprotein drug delivery with a higher initial burst from PLGA50 50 A slow release period has been observed for PLGA75 50 encapsulating a glycoprotein (120572-1-antitrypsin) withclinic activity in some pulmonary diseases [60]
On the other hand a faster erosion of the microsphereswith reduction in the PLGA molecular weight due to thefacility of water penetration and the subsequent polymerdegradation has been described [83] Schrier et al workingwithmicrospheres prepared by wow using different types ofPLGA analyzed the important role of the molecular weightlactide-glycolide relation and acid residues [57]The amountof rhBMP2 adsorbed on the microparticle surface increased
10 BioMed Research International
with the hydrophobicity of the polymer At the same time therelease was in correlation with the degradation profile of thedifferent polymers [57]
Thus the use of more hydrophilic polymers reduces thehydrophobic protein-polymer interaction This effect favorsa more homogeneous distribution in the polymer matrixand increases the water uptake in the microspheres Thusthe release rate of rhBMP2 encapsulated in microspherescomposed by a PEG-PLGA di-block copolymer is increasedwith the PEG content of the polymer matrix [58] A similarresult was obtained using PLGA-PEG-PLGA triblock copoly-mers [59] In this case modifying the monomer relation(lactide-glycolide) in the PLGA and increasing the amountof PLGA-PEG-PLGA in the formulations the release profileof BMP-2 coencapsulated with human SA in microesphereswas adjustable Similarly the interaction of lysozyme withpoloxamer 188 before their encapsulation produces a sus-tained release over 3 weeks without any burst effect In thesame line using PLGA-PEG-PLGA as polymer a sustainedrelease of bioactive lysozime was extended over 45 days whenthe protein was complexed with poloxamer 188 previously tothe encapsulation [120] However the presence of PEG300 asan additive of the inner phase of microparticles during theencapsulation process also influences the protein distributionand the release profile In this case there is a decrease of theinitial burst but with less overall release [58]
On the other hand the use of PLGA-poloxamers blendsis useful to obtain a sustained release for more than onemonthwithout any incidence in the high initial burst [56 92]However for an encapsulated plasmid inside nanoparticlesobtained by PLGA-poloxamer blends the hydrophobicity ofthe surfactant allows prolonging the release up to 2 weeks in acontrolledmannerMoreover a complete release was reachedfor the PLGA-poloxamer blend instead PLGA nanoparticlesin which the maximum release was around 40 [84]
PLGA and poloxamers (pluronic F68) blends can also beused to obtain nanocomposite vesicles by a double emulsionprocess These vesicles are suitable for the encapsulation ofhydrophobic and hydrophilic molecules The presence ofpluronic affects the colloidal stability of the vesicles and therelease pattern of the encapsulated molecules These vesiclespresent a wall of 30 nm and the drug is encapsulated in thepresence of the poloxamer [121]
Other strategies include the use of different compounds toincrease the release timeThus BMP2 encapsulated in PLGA-PVA nanoparticles (around 300 nm) showed higher encapsu-lation efficiency and a short-time release profile with a veryhigh initial burst However with the same synthesis proce-dure (wow) but using PHBV (Poly(3 hydroxybutyrateco-3-hydroxivalerate)) BMP7 loaded nanocapsules had lessencapsulation efficiency despite a long-time delivery Nev-ertheless the maximum released amount was lower Thisdifference in the release profile was due to the differencein hydrophilicity and degradation rates of both polymers[122] Similarly PLGA-poloxamer blend nanoparticles weresuperficially modified by introducing chitosan in the secondstep of the synthesisThis method showed a sustained releaseprofile for up to 14 days without any initial important burstIn this case a recombinant hepatitis B antigen was used
[106] Moreover the use of heparin conjugated with PLGAporous microspheres has also been described to obtain along-time delivery system reducing at the same time theinitial burst In these systems heparin was immobilized ontothe nanomicroparticle surface The release was controlledby using the binding affinities of heparin to several growthfactors including BMP2 In this case the initial burst wasreduced to 4ndash7 during first day followed by a sustainedrelease of about 1 per day [51ndash53]
The initial burst release may be attenuated by thefabrication of double-wall microspheres that is core-shellmicroparticles The presence of a PLA shell reduces therelease rate of BSA encapsulated in the PLGA core andextends the duration of the release profile up to two monthsMoreover an increase in the PLA molecular weight influ-ences the rate of particle erosion which further slows theprotein release [123]
The modification of the viscosity in the environmentof microparticles additionally influences the release patternViscosity can control the burst at earliest time point andpromote a sustained release This situation has been shownfor rhBMP2-PLGA microspheres embedded in a chitosan-thioglycolic acid hydrogel (Poloxamer 407) [124] Yilgor etal also incorporated the nanoparticles of their sequentialdelivery system into a scaffold composed by chitosan andchitosan-PEO [54] In other work PLGAPVA microsphereswith encapsulated BMP2 were combined with differentcomposite biomaterials (gelatin hydrogel or polypropylenefumarate) The sustained release of the bioactive moleculewas extended over a period of 42 days In vivo results indicatethe importance of the composite characteristics In this casean enhanced bone formation was obtained when the PLGAmicroparticles were incorporated into the more hydrophobicmatrix (polypropylene fumarate) [125 126]
Finally Table 2 summarizes important information aboutdifferent parameters related to the use of PLGA basednano- ormicroparticles to encapsulate transport and releasegrowth factors (mainly BMP2)
36 Gene Therapy for Bone Tissue Engineering DirectedDelivery In the last years gene therapy has begun to playa role in bone tissue regeneration becoming an alternativemethod for the delivery of BMP2 [127 128] Thus the genesencoding a specific protein can be delivered to a specific cellrather than the proteins themselves To reach this purposean efficient gene vector is necessary Viral vectors possess thebest transfection efficiency but numerous disadvantages themost notable of them being the risk of mutagenesis Nonviralvectors elude these problems but with a significant reductionin the transfection rate [129]Therefore intracellular deliveryof bioactive agents has become the most used strategy forgene therapy looking for the adequate transfection andconsequent expression of the desired protein [79]
PLGA microspheres obtained by a wow double emul-sion process have been used by Qiao et al to entrap plasmid-BMP2polyethyleneimine nanoparticles In this case a sus-tained release of these nanoparticles until 35 days without ini-tial burst was found resulting in differentiation of osteoblast
BioMed Research International 11
Table 2 Nanomicroparticles systems to encapsulate GFs mainly BMP2 growth factor Most of them are in the microscopic scale andwere used to be entrapped into scaffold of different characteristics PVA has been the more used surfactant-stabilizer It is possible to findboth encapsulation and surface adsorption of the growth factors with high-moderate efficiency The use of heparin as stabilizer reducessignificantly the initial burst release favoring a sustained release in the time The bioactivity of the GF was preserved in most of the systemsand coencapsulation with other biomolecules seems to have a similar effect than the use of surfactants as stabilizers
Polymers Stabilizer Size Encapsulation EE Release Biological activity Reference
PLGA PVA 10ndash20120583m AdsorbedrhBMP2
20 ngmL ofconstant sustained
release
Better boneformation after 8
weeksFu et al 2013 [44]
PLGA PVA 10ndash100120583m rhBMP2-BSA69 (BMP)
Burst (20)Sustained until77 (28 days)
BMP2 moleculeswith bioactivity Tian et al 2012 [45]
PLGA 75 25 PVA 182120583m 82 mdash
Good bonedefect repair
outcomes within8minus12 weeks
Rodrıguez-Evoraet al 2014 [46]
PLGA PVA 228120583m 605
30 initial burstSlower release of4 per week After
8 weeks 60released
No loss ofbioactivity
Reyes et al 2013[47]
PLGAPEGNo doubleemulsionsynthesis
100ndash200 120583m Adsorbed BMP2
13 initial burstSlower release of001ndash8 per dayAfter 23 days 70
released
Substantial boneregeneration of the
scaffold
Rahman et al 2014[48]
Different PLGA PVA 20ndash100 120583m
30 (uncappedPLGA)
90 (cappedPLGA)
26ndash49 (1 day)Total after 2 weeks
No loss ofbioactivity
Lupu-Haber et al2013 [49]
PLGA 75 25 PVA 5ndash125 120583m mdashInitial burst 30 (1
day)Sustained 35 days
Higher volumesand surface areacoverage of new
bone
Wink et al 2014[50]
PLGA Heparin 200ndash800 nm Adsorbed BMP294
No initial burstSustained over 4
weeks
Significantreduction of theBMP2 dose forgood boneformation
La et al 2010 [51]
PLGA Heparin-Poloxamer 160 nm Adsorbed BMP2
100
Initial burst(4ndash7) linear
profile
Higher matrixmineralization ofregenerated bone
Chung et al 2007[52]
PLGA Heparin 100ndash250 nm Adsorbed 94Initial burst 10 (1
day)60 after 30 days
No loss ofbioactivityEfficacy of
administrationamount 50-fold
lower
Jeon et al 2008 [53]
PLGA PVA sim300 nm 80 85 initial burst (1day)
No loss ofbioactivity
Yilgor et al 2009[54]
PLGA (in rings) PVA 215 120583m 66Moderate burstSustained releaseover 6 weeks
60 of calvariadefect were healed
Rodrıguez-Evoraet al 2013 [55]
PLGA-Poloxamer 188Blend
Poloxamer 150 nmFGF-BSA-Heparin60ndash80
40 initial burst (1day) 60 (30 days)
No loss ofbioactivity
drsquoAngelo et al 2010[56]
Different PLGApolymers PVA 120583m order
rhBMP2adsorption40ndash75
20ndash80 initialburst (1 day) mdash Schrier et al 2001
[57]
12 BioMed Research International
Table 2 Continued
Polymers Stabilizer Size Encapsulation EE Release Biological activity Reference
PLGAPEG PVA 37ndash67 120583m 72ndash99 33 initial burst (1day)
Little loss ofbioactivity
Lochmann et al2010 [58]
PLGAPLGA-PEG-PLGA PVA 100 120583m HSA-BMP2
6070 initial burst (1
day)No loss ofbioactivity
White et al 2013[59]
PLGA PVA 100ndash1000 nm Α-1-antitrypsin90
30 initial burst (1day)
50 after 24 days
Biological activitywas preservedusing BSA and120573-cyclodextrine
Pirooznia et al2012 [60]
promoted by the correct transfection of the delivered bio-functional BMP2-DNA [130]
In spite of the general caution with gene therapy thegenetic delivery of BMP2 has the potentiality of a better safetycompared with the delivery of large amounts of recombinantprotein [131] Lu et al specify the urgent need to developmoreefficient delivery nanoparticles and transfection methods inorder to apply the nonviral vectors in stem cell engineeringand bone regeneration Although enhanced bone formationhas been shown in several recent studies using genes suchas HIF-1120572 and miRNAs new genetic sequences will bediscovered and used in bone engineering in the near futurethat will most likely change our perspective [132]
PLGA nanospheres represent a well-studied biomoleculedelivery system that could be applied to cell targeting inorder to enhance the delivery of specific proteins or nucleicacids inside or near the bone engineering reference cells thatis mesenchymal stem cells [133]The targeting properties canbe supplied by a ligand functionalization strategy modifica-tion of the surface structure of the nanocarrier by conjugatinga cell-specific ligand to direct the release of encapsulatedbiomolecules preferably in close association with the targetcells [134]The use of pegylated nanoparticles with a covalentattachment of different ligands is reported as a potentialtechnique to deliver bone cell-specific biomolecules for boneengineering [135]
Specific antibodies that recognize surface receptors inthese cells could be covalently coupled to the surface of PLGAnanoparticles obtaining ldquoimmunonanoparticlesrdquo There areseveral examples of antibody immobilization on surfaceof PLGA nanoparticles Kocbek et al demonstrated thespecific recognition of breast tumor cells by a specific mono-clonal antibody attached on PLGA fluorescent nanoparticlesobtained by WOW emulsion process [136] For the surfacecovalent attachment they used a more simple carbodiimidemethod which promotes the formation of an amide bondbetween free carboxylic end groups of PLGA nanoparticlesand primary amine groups of the antibody molecule [81]This procedure can be highly influenced by the presenceof stabilizers frequently used to confer colloidal stabilityto nanoparticles The electrophoretic mobility of PLGAnanoparticles with an antibody (immuno-120574-globuline anti-human C-reactive protein) covalently attached on the surfaceis shown in Figure 5 It is necessary to remark the drasticdecrease in the mobility values of the antibody-modified
nanoparticles with respect to bare PLGA nanoparticleswhich could imply low colloidal stability and the subse-quent aggregation of the nanosystem Santander-Ortega etal proposed a lower antibody loading in which the barePLGA patches must be coated by a nonionic surfactant inorder to obtain immunoreactive stable nanoparticles [95]Ratzinger et al indicated that the presence of high polox-amer concentrations decreased the coupling efficiency tocarboxylic end groups in PLGA nanoparticles showing thatan equilibrium that combines sufficient stability and the bestcoupling efficiency is necessary [98] To prevent this problemCheng et al synthetized carboxyl functionalized PLGA-PEG block copolymer attaching a specific aptamer to thesurface of pegylated nanoparticles via carbodiimide methodIn this work an enhanced drug delivery to prostate tumorshas been shown in comparison to equivalent nontargetednanoparticles [137]
37 Scaffolds The data reported in the literature indicatethat PLGA micronanoparticles are promising to achievea sustained spatial and temporally controlled delivery ofgrowth factors required for cell growth and cell differen-tiation They can be incorporated with cells in solid scaf-fold or injectable hydrogels [73] Scaffolds are porous 3Dstructures normally used to improve tissue-engineered bone[28] According to Tian et al [45] a scaffold designedwith this objective must have (1) appropriate mechanicalstrength to support the growth of new bone (2) appropriateporosity to allow ingrowth of bone-related cells (3) goodbiocompatibility allowing the growth of cells on its surfacewithout being rejected by the body and (4) low toxicity tocells and tissues surrounded and (5) must be able to induceosteogenic differentiation of bone-related stem cells and (6)be biodegradable with nontoxic degradation products thatcan be eventually replaced by new bone Additionally thescaffold for bone regeneration must maintain the delivery orrelease of BMP (growth factors) ldquoin siturdquo for a long time Inthis way nanomicroparticles inside scaffolds are being usedto release an adequate flow of these signaling biomoleculesand preserve their functional structure [138] The incorpo-ration of colloidal micronanoparticles into fibrous scaffoldsadds in the possibility of multiple drugs loading Howeverthis multidrug system could also involve a decrease ofthe mechanical properties of the structure and a possibleloss of nanoparticles entrapped between the fibers [139]
BioMed Research International 13
Considering that the in vivo half-life of most biomoleculesespecially proteins is relatively short it is essential thatbioactive scaffolds maintain a desired concentration ldquoin siturdquoto direct tissue regeneration To do so an initial release ofthe encapsulated growth factor in the first hours to quicklyget an effective therapeutic concentration followed by asustained long-time release profile is required [139] Most ofthe polymeric particles inserted in scaffold structures are ina micron-scale The main objective of these microparticlesis the protection and temporary control of growth factordelivery However given the porosity of these structuresnanoparticles and especially particles of a few microns maybecome more important since it is possible to design systemswith a simple and easy diffusion through the structure Thisprocess could allow the specific recognition of a particularcell type releasing their encapsulated BMPs in the sameenvironment and helping their differentiation to cellbonetissue In any case the larger-size microspheres might notnecessarily be useless for bone regeneration scaffolds As themicrospheres gradually degrade the space they occupied willbe conducive to ingrowth of tissue In addition to affectingthe compressionmodulus of scaffolds because of their hollowfeature the particle size of microspheres can also influencethe release of rhBMP2 [45]
4 Conclusion
The use of polymeric particles using PLGA is a promisingsystem for a spatially and temporally controlled delivery ofgrowth factors that promote cell growth and differentiationin bone engineering and regeneration by means of theirincorporation beside cells into solid scaffold or hydrogels
The PLGA is widely used for its biodegradability andbiocompatibility and is approved by FDA and the EuropeanMedicines Agency for use in drug delivery systems suppliedvia parenteral On the other hand BMPs are potent growthfactors for bone repair and specifically BMP2 shows excellentability to induce bone formation of adequate quality Theprocedure for synthesizing PLGA nano- or microparticlescan be modified in their different variables to obtain systemswith controlled size in which it is possible to encapsulatehydrophobic or hydrophilic molecules with an adequate col-loidal stability and the possibility of surface functionalizationfor targeted delivery
With this scenario an optimization of methods and com-ponentsmust balance the structure andmorphology of PLGAmicronanoparticles in order to achieve high encapsulationefficiency of BMP2 and looking for a main goal control ofdelivery reducing the initial burst and reaching a sustainedrelease profile preserving the biological activity and directedto the target cells tominimize the clinical amount needed andallowing a correct bone tissue regeneration
Conflict of Interests
The authors declare no conflict of interests with any of theproducts listed in the paper
Acknowledgments
The authors wish to express their appreciation for thefinancial support granted by the ldquoMinisterio de Educaciony Cienciardquo (MEC Spain) Projects MAT2013-43922-R andResearch Groups no FQM-115 no CTS-138 and no CTS-583 (Junta de Andalucıa Spain) Partial support was alsoprovided by the Andalucıa Talent Hub Program from theAndalusian Knowledge Agency cofunded by the EuropeanUnionrsquos Seventh Framework Program Marie Skłodowska-Curie actions (COFUND Grant Agreement no 291780) andthe Ministry of Economy Innovation Science and Employ-ment of the Junta de Andalucıa (Miguel Padial-Molina)
References
[1] M Padial-Molina P Galindo-Moreno and G Avila-OrtizldquoBiomimetic ceramics in implant dentistryrdquoMinerva Biotecno-logica vol 21 no 3 pp 173ndash186 2009
[2] B Al-Nawas and E Schiegnitz ldquoAugmentation proceduresusing bone substitute materials or autogenous bonemdasha sys-tematic review and meta-analysisrdquo European Journal of OralImplantology vol 7 supplement 2 pp S219ndashS234 2014
[3] A Katranji P Fotek and H-L Wang ldquoSinus augmentationcomplications etiology and treatmentrdquo Implant Dentistry vol17 no 3 pp 339ndash349 2008
[4] C E Misch ldquoMaxillary sinus augmentation for endostealimplants organized alternative treatment plansrdquo The Interna-tional Journal of Oral Implantology Implantologist vol 4 no 2pp 49ndash58 1987
[5] C Myeroff and M Archdeacon ldquoAutogenous bone graft donorsites and techniquesrdquo The Journal of Bone amp Joint SurgerymdashAmerican Volume vol 93 no 23 pp 2227ndash2236 2011
[6] G Avila R Neiva C E Misch et al ldquoClinical and histologicoutcomes after the use of a novel allograft for maxillary sinusaugmentation a case seriesrdquo Implant Dentistry vol 19 no 4pp 330ndash341 2010
[7] S J Froum S S Wallace N Elian S C Cho and D P TarnowldquoComparison of mineralized cancellous bone allograft (Puros)and anorganic bovine bonematrix (Bio-Oss) for sinus augmen-tation histomorphometry at 26 to 32 weeks after graftingrdquoTheInternational Journal of Periodontics amp Restorative Dentistryvol 26 no 6 pp 543ndash551 2006
[8] P Galindo-Moreno G Avila J E Fernandez-Barbero et alldquoEvaluation of sinus floor elevation using a composite bone graftmixturerdquo Clinical Oral Implants Research vol 18 no 3 pp 376ndash382 2007
[9] P Galindo-Moreno I Moreno-Riestra G Avila et al ldquoEffectof anorganic bovine bone to autogenous cortical bone ratioupon bone remodeling patterns following maxillary sinusaugmentationrdquo Clinical Oral Implants Research vol 22 no 8pp 857ndash864 2011
[10] S L Wheeler ldquoSinus augmentation for dental implants theuse of alloplastic materialsrdquo Journal of Oral and MaxillofacialSurgery vol 55 no 11 pp 1287ndash1293 1997
[11] S S Wallace and S J Froum ldquoEffect of maxillary sinusaugmentation on the survival of endosseous dental implantsA systematic reviewrdquo Annals of Periodontologythe AmericanAcademy of Periodontology vol 8 no 1 pp 328ndash343 2003
14 BioMed Research International
[12] M Padial-Molina and H F Rios ldquoStem cells scaffolds andgene therapy for periodontal engineeringrdquo Current Oral HealthReports vol 1 no 1 pp 16ndash25 2014
[13] M Padial-Molina S L Volk and H F Rios ldquoPeriostinincreases migration and proliferation of human periodontalligament fibroblasts challenged by tumor necrosis factor -alphaand Porphyromonas gingivalis lipopolysaccharidesrdquo Journal ofPeriodontal Research vol 49 no 3 pp 405ndash414 2014
[14] H Behnia A Khojasteh M Soleimani A Tehranchi and AAtashi ldquoRepair of alveolar cleft defect with mesenchymal stemcells and platelet derived growth factors a preliminary reportrdquoJournal of Cranio-Maxillofacial Surgery vol 40 no 1 pp 2ndash72012
[15] M Padial-Molina J T Marchesan A D Taut Q Jin WV Giannobile and H F Rios ldquoMethods to validate tooth-supporting regenerative therapiesrdquo Methods in Molecular Biol-ogy vol 887 pp 135ndash148 2012
[16] M R Urist ldquoBone formation by autoinductionrdquo Science vol150 no 3698 pp 893ndash899 1965
[17] P Boyne and S D Jones ldquoDemonstration of the osseoinductiveeffect of bone morphogenetic protein within endosseous dentalimplantsrdquo Implant Dentistry vol 13 no 2 pp 180ndash184 2004
[18] E A Wang V Rosen J S DrsquoAlessandro et al ldquoRecombinanthuman bone morphogenetic protein induces bone formationrdquoProceedings of the National Academy of Sciences of the UnitedStates of America vol 87 no 6 pp 2220ndash2224 1990
[19] J M Wozney ldquoThe bone morphogenetic protein family andosteogenesisrdquoMolecular Reproduction andDevelopment vol 32no 2 pp 160ndash167 1992
[20] E Barboza A Caula and F Machado ldquoPotential of recombi-nant human bone morphogenetic protein-2 in bone regenera-tionrdquo Implant Dentistry vol 8 no 4 pp 360ndash367 1999
[21] A C Carreira G G Alves W F Zambuzzi M C Sogayarand J M Granjeiro ldquoBone morphogenetic proteins structurebiological function and therapeutic applicationsrdquo Archives ofBiochemistry and Biophysics vol 561 pp 64ndash73 2014
[22] J C Bustos-Valenzuela A Fujita E Halcsik J M Granjeiroand M C Sogayar ldquoUnveiling novel genes upregulated by bothrhBMP2 and rhBMP7 during early osteoblastic transdifferen-tiation of C2C12 cellsrdquo BMC Research Notes vol 4 article 3702011
[23] K Tsuji A Bandyopadhyay B D Harfe et al ldquoBMP2 activityalthough dispensable for bone formation is required for theinitiation of fracture healingrdquo Nature Genetics vol 38 no 12pp 1424ndash1429 2006
[24] K Tsuji K Cox A Bandyopadhyay B D Harfe C J Tabin andV Rosen ldquoBMP4 is dispensable for skeletogenesis and fracture-healing in the limbrdquo The Journal of Bone and Joint SurgerymdashAmerican Volume vol 90 supplement 1 pp 14ndash18 2008
[25] K Tsuji K Cox L Gamer D Graf A Economides and VRosen ldquoConditional deletion of BMP7 from the limb skeletondoes not affect bone formation or fracture repairrdquo Journal ofOrthopaedic Research vol 28 no 3 pp 384ndash389 2010
[26] G Chen C Deng and Y-P Li ldquoTGF-beta and BMP signalingin osteoblast differentiation and bone formationrdquo InternationalJournal of Biological Sciences vol 8 no 2 pp 272ndash288 2012
[27] M-C Ramel and C S Hill ldquoSpatial regulation of BMP activityrdquoFEBS Letters vol 586 no 14 pp 1929ndash1941 2012
[28] A C Carreira F H Lojudice E Halcsik R D Navarro M CSogayar and J M Granjeiro ldquoBone morphogenetic proteinsfacts challenges and future perspectivesrdquo Journal of DentalResearch vol 93 no 4 pp 335ndash345 2014
[29] F Deschaseaux L Sensebe and D Heymann ldquoMechanisms ofbone repair and regenerationrdquo Trends in Molecular Medicinevol 15 no 9 pp 417ndash429 2009
[30] T DMueller and J Nickel ldquoPromiscuity and specificity in BMPreceptor activationrdquo FEBS Letters vol 586 no 14 pp 1846ndash1859 2012
[31] C Sieber J Kopf C Hiepen and P Knaus ldquoRecent advancesin BMP receptor signalingrdquo Cytokine amp Growth Factor Reviewsvol 20 no 5-6 pp 343ndash355 2009
[32] G Sapkota C Alarcon F M Spagnoli A H Brivanlou and JMassague ldquoBalancing BMP signaling through integrated inputsinto the Smad1 linkerrdquoMolecular Cell vol 25 no 3 pp 441ndash4542007
[33] W F McKay S M Peckham and J M Badura ldquoA comprehen-sive clinical review of recombinant human bonemorphogeneticprotein-2 (INFUSE Bone Graft)rdquo International Orthopaedicsvol 31 no 6 pp 729ndash734 2007
[34] P Hong D Boyd S D Beyea and M Bezuhly ldquoEnhancementof bone consolidation in mandibular distraction osteogenesisa contemporary review of experimental studies involving adju-vant therapiesrdquo Journal of Plastic Reconstructive and AestheticSurgery vol 66 no 7 pp 883ndash895 2013
[35] D B Spagnoli and R E Marx ldquoDental implants and the use ofrhBMP-2rdquo Dental Clinics of North America vol 55 no 4 pp883ndash907 2011
[36] M Nevins C Kirker-HeadM Nevins J AWozney R Palmerand D Graham ldquoBone formation in the goat maxillary sinusinduced by absorbable collagen sponge implants impregnatedwith recombinant human bone morphogenetic protein-2rdquo TheInternational Journal of Periodontics amp Restorative Dentistryvol 16 no 1 pp 8ndash19 1996
[37] P J Boyne L C Lilly R E Marx et al ldquoDe novo bone induc-tion by recombinant human bone morphogenetic protein-2(rhBMP-2) in maxillary sinus floor augmentationrdquo Journal ofOral and Maxillofacial Surgery vol 63 no 12 pp 1693ndash17072005
[38] L Torrecillas-Martinez A Monje M A Pikos et al ldquoEffect ofrhBMP-2 uponmaxillary sinus augmentation a comprehensivereviewrdquo Implant Dentistry vol 22 no 3 pp 232ndash237 2013
[39] J Lee C Susin N A Rodriguez et al ldquoSinus augmentationusing rhBMP-2ACS in a mini-pig model relative efficacy ofautogenous fresh particulate iliac bone graftsrdquo Clinical OralImplants Research vol 24 no 5 pp 497ndash504 2013
[40] RG TriplettMNevins R EMarx et al ldquoPivotal randomizedparallel evaluation of recombinant human bonemorphogeneticprotein-2absorbable collagen sponge and autogenous bonegraft for maxillary sinus floor augmentationrdquo Journal of Oraland Maxillofacial Surgery vol 67 no 9 pp 1947ndash1960 2009
[41] D W K Kao A Kubota M Nevins and J P Fiorellini ldquoThenegative effect of combining rhBMP-2 and Bio-Oss on boneformation for maxillary sinus augmentationrdquoThe InternationalJournal of Periodontics amp Restorative Dentistry vol 32 no 1 pp61ndash67 2012
[42] O Hanisch D N Tatakis M D Rohrer P S Wohrle JM Wozney and U M E Wikesjo ldquoBone formation andosseointegration stimulated by rhBMP-2 following subantralaugmentation procedures in nonhuman primatesrdquoThe Interna-tional Journal of Oral ampMaxillofacial Implants vol 12 no 6 pp785ndash792 1997
[43] K Wada A Niimi K Watanabe T Sawai and M UedaldquoMaxillary sinus floor augmentation in rabbits a comparative
BioMed Research International 15
histologic-histomorphometric study between rhBMP-2 andautogenous bonerdquo The International Journal of Periodontics ampRestorative Dentistry vol 21 no 3 pp 253ndash263 2001
[44] R Fu S Selph M McDonagh et al ldquoEffectiveness and harmsof recombinant human bone morphogenetic protein-2 in spinefusion a systematic review and meta-analysisrdquo Annals of Inter-nal Medicine vol 158 no 12 pp 890ndash902 2013
[45] Z Tian Y Zhu J Qiu et al ldquoSynthesis and characterization ofUPPE-PLGA-rhBMP2 scaffolds for bone regenerationrdquo Journalof Huazhong University of Science and TechnologymdashMedicalScience vol 32 no 4 pp 563ndash570 2012
[46] M Rodrıguez-Evora E Garcıa-Pizarro C del Rosario et alldquoSmurf1 knocked-down mesenchymal stem cells and BMP-2 in an electrospun system for bone regenerationrdquo Biomacro-molecules vol 15 no 4 pp 1311ndash1322 2014
[47] R Reyes A Delgado R Solis et al ldquoCartilage repair bylocal delivery of TGF-1205731 or BMP-2 from a novel segmentedpolyurethanepolylactic-co-glycolic bilayered scaffoldrdquo Journalof Biomedical Materials Research Part A 2013
[48] C V Rahman D Ben-David A Dhillon et al ldquoControlledrelease of BMP-2 from a sintered polymer scaffold enhancesbone repair in a mouse calvarial defect modelrdquo Journal of TissueEngineering and Regenerative Medicine vol 8 no 1 pp 59ndash662014
[49] Y Lupu-Haber O Pinkas S Boehm T Scheper C Kasper andM Machluf ldquoFunctionalized PLGA-doped zirconium oxideceramics for bone tissue regenerationrdquoBiomedicalMicrodevicesvol 15 no 6 pp 1055ndash1066 2013
[50] J D Wink P A Gerety R D Sherif et al ldquoSustaineddelivery of rhBMP-2 by means of poly(lactic-co-glycolic acid)microspheres cranial bone regeneration without heterotopicossification or craniosynostosisrdquo Plastic and ReconstructiveSurgery vol 134 no 1 pp 51ndash59 2014
[51] W-G La S-W Kang H S Yang et al ldquoThe efficacy of bonemorphogenetic protein-2 depends on its mode of deliveryrdquoArtificial Organs vol 34 no 12 pp 1150ndash1153 2010
[52] Y-I Chung K-M Ahn S-H Jeon S-Y Lee J-H Lee and GTae ldquoEnhanced bone regeneration with BMP-2 loaded func-tional nanoparticle-hydrogel complexrdquo Journal of ControlledRelease vol 121 no 1-2 pp 91ndash99 2007
[53] O Jeon S J Song H S Yang et al ldquoLong-term deliveryenhances in vivo osteogenic efficacy of bone morphogeneticprotein-2 compared to short-term deliveryrdquo Biochemical andBiophysical Research Communications vol 369 no 2 pp 774ndash780 2008
[54] P Yilgor K Tuzlakoglu R L Reis N Hasirci and V HasircildquoIncorporation of a sequential BMP-2BMP-7 delivery systeminto chitosan-based scaffolds for bone tissue engineeringrdquoBiomaterials vol 30 no 21 pp 3551ndash3559 2009
[55] M Rodrıguez-Evora A Delgado R Reyes et al ldquoOsteogeniceffect of local long versus short term BMP-2 delivery froma novel SPU-PLGA-120573TCP concentric system in a critical sizedefect in ratsrdquo European Journal of Pharmaceutical Sciences vol49 no 5 pp 873ndash884 2013
[56] I drsquoAngelo M Garcia-Fuentes Y Parajo et al ldquoNanoparticlesbased on PLGA poloxamer blends for the delivery of proangio-genic growth factorsrdquoMolecular Pharmaceutics vol 7 no 5 pp1724ndash1733 2010
[57] J A Schrier B F Fink J B Rodgers H C Vasconez and PP DeLuca ldquoEffect of a freeze-dried CMCPLGA microspherematrix of rhBMP-2 on bone healingrdquo AAPS PharmSciTech vol2 no 3 article E18 2001
[58] A Lochmann H Nitzsche S von Einem E Schwarz and KMader ldquoThe influence of covalently linked and free polyethy-lene glycol on the structural and release properties of rhBMP-2loaded microspheresrdquo Journal of Controlled Release vol 147 no1 pp 92ndash100 2010
[59] L J White G T S Kirby H C Cox et al ldquoAcceleratingprotein release from microparticles for regenerative medicineapplicationsrdquoMaterials Science and Engineering C Materials forBiological Applications vol 33 no 5 pp 2578ndash2583 2013
[60] N Pirooznia S Hasannia A S Lotfi and M Ghanei ldquoEncap-sulation of alpha-1 antitrypsin in PLGA nanoparticles in vitrocharacterization as an effective aerosol formulation in pul-monary diseasesrdquo Journal of Nanobiotechnology vol 10 article20 2012
[61] M Ronga A Fagetti G Canton E Paiusco M F Surace andP Cherubino ldquoClinical applications of growth factors in boneinjuries experience with BMPsrdquo Injury vol 44 supplement 1pp S34ndashS39 2013
[62] J G Devine J R Dettori J C France E Brodt and R AMcGuire ldquoThe use of rhBMP in spine surgery is there a cancerriskrdquo Evidence-Based Spine-Care Journal vol 3 no 2 pp 35ndash41 2012
[63] E J Carragee E L Hurwitz and B KWeiner ldquoA critical reviewof recombinant human bone morphogenetic protein-2 trials inspinal surgery emerging safety concerns and lessons learnedrdquoThe Spine Journal vol 11 no 6 pp 471ndash491 2011
[64] M C Simmonds J V E Brown M K Heirs et al ldquoSafetyand effectiveness of recombinant human bone morphogeneticprotein-2 for spinal fusion a meta-analysis of individual-participant datardquo Annals of Internal Medicine vol 158 no 12pp 877ndash889 2013
[65] V H-Y Chung A Y-L Chen L-B Jeng C-C Kwan S-H Cheng and S C-N Chang ldquoEngineered autologous bonemarrow mesenchymal stem cells alternative to cleft alveolarbone graft surgeryrdquo Journal of Craniofacial Surgery vol 23 no5 pp 1558ndash1563 2012
[66] T A Ratko S E Belinson D J Samson C Bonnell K MZiegler and N Aronson BoneMorphogenetic ProteinThe Stateof the Evidence of On-Label and Off-Label Use Agency forHealthcare Research and Quality Rockville Md USA 2010
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[68] V W Bramwell and Y Perrie ldquoParticulate delivery systems forvaccinesrdquo Critical Reviews inTherapeutic Drug Carrier Systemsvol 22 no 2 pp 151ndash214 2005
[69] N Csaba M Garcia-Fuentes and M J Alonso ldquoThe perfor-mance of nanocarriers for transmucosal drug deliveryrdquo ExpertOpinion on Drug Delivery vol 3 no 4 pp 463ndash478 2006
[70] M J Santander-Ortega T Stauner B Loretz et al ldquoNanopar-ticles made from novel starch derivatives for transdermal drugdeliveryrdquo Journal of Controlled Release vol 141 no 1 pp 85ndash922010
[71] W Jiang R K Gupta M C Deshpande and S P Schwende-man ldquoBiodegradable poly(lactic-co-glycolic acid) microparti-cles for injectable delivery of vaccine antigensrdquo Advanced DrugDelivery Reviews vol 57 no 3 pp 391ndash410 2005
[72] T R Shantha Kumar K Soppimath and S K NachaegarildquoNovel delivery technologies for protein and peptide therapeu-ticsrdquo Current Pharmaceutical Biotechnology vol 7 no 4 pp261ndash276 2006
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[73] F Danhier E Ansorena J M Silva R Coco A Le Bretonand V Preat ldquoPLGA-based nanoparticles an overview ofbiomedical applicationsrdquo Journal of Controlled Release vol 161no 2 pp 505ndash522 2012
[74] A Kumari S K Yadav and S C Yadav ldquoBiodegradablepolymeric nanoparticles based drug delivery systemsrdquo Colloidsand Surfaces B Biointerfaces vol 75 no 1 pp 1ndash18 2010
[75] H K Makadia and S J Siegel ldquoPoly Lactic-co-Glycolic Acid(PLGA) as biodegradable controlled drug delivery carrierrdquoPolymers vol 3 no 3 pp 1377ndash1397 2011
[76] F Mohamed and C F van der Walle ldquoEngineering biodegrad-able polyester particles with specific drug targeting and drugrelease propertiesrdquo Journal of Pharmaceutical Sciences vol 97no 1 pp 71ndash87 2008
[77] G A Silva O P Coutinho P Ducheyne andR L Reis ldquoMateri-als in particulate form for tissue engineering 2 Applications inbonerdquo Journal of Tissue Engineering and Regenerative Medicinevol 1 no 2 pp 97ndash109 2007
[78] S Zhang and H Uludag ldquoNanoparticulate systems for growthfactor deliveryrdquoPharmaceutical Research vol 26 no 7 pp 1561ndash1580 2009
[79] V E Santo M E Gomes J F Mano and R L Reis ldquoFromnano-to macro-scale nanotechnology approaches for spatiallycontrolled delivery of bioactive factors for bone and cartilageengineeringrdquo Nanomedicine vol 7 no 7 pp 1045ndash1066 2012
[80] M-K Tran A Swed and F Boury ldquoPreparation of polymericparticles in CO
2medium using non-toxic solvents formulation
and comparisons with a phase separation methodrdquo EuropeanJournal of Pharmaceutics and Biopharmaceutics vol 82 no 3pp 498ndash507 2012
[81] B Ertl F Heigl M Wirth and F Gabor ldquoLectin-mediatedbioadhesion preparation stability andCaco-2 binding of wheatgerm agglutinin-functionalized poly-(DL-lactic-co-glycolicacid)-microspheresrdquo Journal of Drug Targeting vol 8 no 3 pp173ndash184 2000
[82] M L Hans and A M Lowman ldquoBiodegradable nanoparticlesfor drug delivery and targetingrdquo Current Opinion in Solid Stateand Materials Science vol 6 no 4 pp 319ndash327 2002
[83] D Blanco and M J Alonso ldquoProtein encapsulation and releasefrom poly(lactide-co-glycolide) microspheres effect of the pro-tein and polymer properties and of the co-encapsulation ofsurfactantsrdquo European Journal of Pharmaceutics and Biophar-maceutics vol 45 no 3 pp 285ndash294 1998
[84] N Csaba P Caamano A Sanchez F Domınguez and MJ Alonso ldquoPLGA poloxamer and PLGApoloxamine blendnanoparticles new carriers for gene deliveryrdquo Biomacro-molecules vol 6 no 1 pp 271ndash278 2005
[85] C Sturesson and J Carlfors ldquoIncorporation of protein inPLG-microspheres with retention of bioactivityrdquo Journal ofControlled Release vol 67 no 2-3 pp 171ndash178 2000
[86] I D Rosca F Watari and M Uo ldquoMicroparticle formationand its mechanism in single and double emulsion solventevaporationrdquo Journal of Controlled Release vol 99 no 2 pp271ndash280 2004
[87] F T Meng G H Ma W Qiu and Z G Su ldquoWOW doubleemulsion technique using ethyl acetate as organic solventeffects of its diffusion rate on the characteristics of micropar-ticlesrdquo Journal of Controlled Release vol 91 no 3 pp 407ndash4162003
[88] R Ghaderi and J Carlfors ldquoBiological activity of lysozyme afterentrapment in poly (dl-lactide-co-glycolide)-microspheresrdquoPharmaceutical Research vol 14 no 11 pp 1556ndash1562 1997
[89] H Wang S C G Leeuwenburgh Y Li and J A Jansen ldquoTheuse of micro- and nanospheres as functional components forbone tissue regenerationrdquo Tissue Engineering Part B Reviewsvol 18 no 1 pp 24ndash39 2012
[90] S Xiong X Zhao B C Heng K W Ng and J S-C LooldquoCellular uptake of Poly-(DL-lactide-co-glycolide) (PLGA)nanoparticles synthesized through solvent emulsion evapora-tion and nanoprecipitation methodrdquo Biotechnology Journal vol6 no 5 pp 501ndash508 2011
[91] L Y T Chou K Ming and W C W Chan ldquoStrategies forthe intracellular delivery of nanoparticlesrdquo Chemical SocietyReviews vol 40 no 1 pp 233ndash245 2011
[92] M J Santander-Ortega M V Lozano-Lopez D Bastos-Gonzalez J M Peula-Garcıa and J L Ortega-Vinuesa ldquoNovelcore-shell lipid-chitosan and lipid-poloxamer nanocapsulesstability by hydration forcesrdquo Colloid and Polymer Science vol288 no 2 pp 159ndash172 2010
[93] Y-Y Yang T-S Chung and N Ping Ng ldquoMorphology drugdistribution and in vitro release profiles of biodegradable poly-meric microspheres containing protein fabricated by double-emulsion solvent extractionevaporation methodrdquo Biomateri-als vol 22 no 3 pp 231ndash241 2001
[94] T Feczko J Toth and J Gyenis ldquoComparison of the prepara-tion of PLGA-BSA nano- and microparticles by PVA polox-amer and PVPrdquo Colloids and Surfaces A Physicochemical andEngineering Aspects vol 319 no 1ndash3 pp 188ndash195 2008
[95] M J Santander-Ortega D Bastos-Gonzalez and J L Ortega-Vinuesa ldquoElectrophoretic mobility and colloidal stability ofPLGA particles coated with IgGrdquo Colloids and Surfaces BBiointerfaces vol 60 no 1 pp 80ndash88 2007
[96] Y-Y Yang H-H Chia and T-S Chung ldquoEffect of prepara-tion temperature on the characteristics and release profiles ofPLGA microspheres containing protein fabricated by double-emulsion solvent extractionevaporation methodrdquo Journal ofControlled Release vol 69 no 1 pp 81ndash96 2000
[97] D-L Fang Y Chen B Xu et al ldquoDevelopment of lipid-shell andpolymer core nanoparticles with water-soluble salidroside foranti-cancer therapyrdquo International Journal ofMolecular Sciencesvol 15 no 3 pp 3373ndash3388 2014
[98] G Ratzinger U Langer L Neutsch F Pittner M Wirth and FGabor ldquoSurface modification of PLGA particles the interplaybetween stabilizer ligand size and hydrophobic interactionsrdquoLangmuir vol 26 no 3 pp 1855ndash1859 2010
[99] S-S Feng and G Huang ldquoEffects of emulsifiers on thecontrolled release of paclitaxel (Taxol) from nanospheres ofbiodegradable polymersrdquo Journal of Controlled Release vol 71no 1 pp 53ndash69 2001
[100] JMChan L ZhangK P Yuet et al ldquoPLGA-lecithin-PEGcore-shell nanoparticles for controlled drug deliveryrdquo Biomaterialsvol 30 no 8 pp 1627ndash1634 2009
[101] M Fraylich W Wang K Shakesheff C Alexander andB Saunders ldquoPoly(DL-lactide-co-glycolide) dispersions con-taining pluronics from particle preparation to temperature-triggered aggregationrdquo Langmuir vol 24 no 15 pp 7761ndash77682008
[102] M J Santander-Ortega J M Peula-Garcıa F M Goycooleaand J L Ortega-Vinuesa ldquoChitosan nanocapsules effect ofchitosan molecular weight and acetylation degree on electroki-netic behaviour and colloidal stabilityrdquo Colloids and Surfaces BBiointerfaces vol 82 no 2 pp 571ndash580 2011
[103] J S Tan D E Butterfield C L Voycheck K D Caldwell and JT Li ldquoSurfacemodification of nanoparticles by PEOPPOblock
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copolymers to minimize interactions with blood componentsand prolong blood circulation in ratsrdquo Biomaterials vol 14 no11 pp 823ndash833 1993
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[105] R Gref Y Minamitake M T Peracchia V Trubetskoy VTorchilin and R Langer ldquoBiodegradable long-circulating poly-meric nanospheresrdquo Science vol 263 no 5153 pp 1600ndash16031994
[106] P Paolicelli C Prego A Sanchez and M J Alonso ldquoSurface-modified PLGA-based nanoparticles that can efficiently asso-ciate and deliver virus-like particlesrdquo Nanomedicine vol 5 no6 pp 843ndash853 2010
[107] I Brigger C Dubernet and P Couvreur ldquoNanoparticles incancer therapy and diagnosisrdquoAdvancedDrugDelivery Reviewsvol 54 no 5 pp 631ndash651 2002
[108] A Giteau M C Venier-Julienne A Aubert-Pouessel and J PBenoit ldquoHow to achieve sustained and complete protein releasefrom PLGA-based microparticlesrdquo International Journal ofPharmaceutics vol 350 no 1-2 pp 14ndash26 2008
[109] E R Balmayor G A Feichtinger H S Azevedo Mvan Griensven and R L Reis ldquoStarch-poly-120576-caprolactonemicroparticles reduce the needed amount of BMP-2rdquo ClinicalOrthopaedics and Related Research vol 467 no 12 pp 3138ndash3148 2009
[110] M J Santander-Ortega D Bastos-Gonzalez J L Ortega-Vinuesa andM J Alonso ldquoInsulin-loaded PLGA nanoparticlesfor oral administration an in vitro physico-chemical character-izationrdquo Journal of Biomedical Nanotechnology vol 5 no 1 pp45ndash53 2009
[111] L Meinel O E Illi J Zapf M Malfanti H Peter Merkleand B Gander ldquoStabilizing insulin-like growth factor-I inpoly(DL-lactide-co-glycolide) microspheresrdquo Journal of Con-trolled Release vol 70 no 1-2 pp 193ndash202 2001
[112] C Srinivasan Y K Katare T Muthukumaran and A K PandaldquoEffect of additives on encapsulation efficiency stability andbioactivity of entrapped lysozyme from biodegradable polymerparticlesrdquo Journal of Microencapsulation vol 22 no 2 pp 127ndash138 2005
[113] M Tobıo S P Schwendeman Y Guo J McIver R Langerand M J Alonso ldquoImproved immunogenicity of a core-coatedtetanus toxoid delivery systemrdquoVaccine vol 18 no 7-8 pp 618ndash622 1999
[114] D K Malik S Baboota A Ahuja S Hasan and J Ali ldquoRecentadvances in protein and peptide drug delivery systemsrdquoCurrentDrug Delivery vol 4 no 2 pp 141ndash151 2007
[115] J L Cleland ldquoProtein delivery from biodegradable micro-spheresrdquo in Protein Delivery Physical Systems L M Sandersand R W Hendron Eds pp 1ndash41 Plenum Press New YorkNY USA 1997
[116] S H Oh T H Kim and J H Lee ldquoCreating growth factorgradients in three dimensional porous matrix by centrifugationand surface immobilizationrdquo Biomaterials vol 32 no 32 pp8254ndash8260 2011
[117] B N Brown J E Valentin A M Stewart-Akers G P McCabeand S F Badylak ldquoMacrophage phenotype and remodelingoutcomes in response to biologic scaffolds with and without acellular componentrdquo Biomaterials vol 30 no 8 pp 1482ndash14912009
[118] B N Brown J M Freund L Han et al ldquoComparison of threemethods for the derivation of a biologic scaffold composed ofadipose tissue extracellularmatrixrdquoTissue EngineeringmdashPart CMethods vol 17 no 4 pp 411ndash421 2011
[119] B Li T Yoshii A E Hafeman J S Nyman J C Wenkeand S A Guelcher ldquoThe effects of rhBMP-2 released frombiodegradable polyurethanemicrosphere composite scaffoldson new bone formation in rat femorardquo Biomaterials vol 30 no35 pp 6768ndash6779 2009
[120] A Paillard-Giteau V T Tran O Thomas et al ldquoEffect ofvarious additives and polymers on lysozyme release fromPLGAmicrospheres prepared by an sow emulsion techniquerdquoEuropean Journal of Pharmaceutics and Biopharmaceutics vol75 no 2 pp 128ndash136 2010
[121] B P Nair and C P Sharma ldquoPoly(lactide-co-glycolide)-laponite-F68 nanocomposite vesicles through a single-stepdouble-emulsion method for the controlled release of doxoru-bicinrdquo Langmuir vol 28 no 9 pp 4559ndash4564 2012
[122] P Yilgor N Hasirci and V Hasirci ldquoSequential BMP-2BMP-7 delivery from polyester nanocapsulesrdquo Journal of BiomedicalMaterials ResearchmdashPart A vol 93 no 2 pp 528ndash536 2010
[123] Y Xia Q Xu C-H Wang and D W Pack ldquoProtein encapsu-lation in and release from monodisperse double-wall polymermicrospheresrdquo Journal of Pharmaceutical Sciences vol 102 no5 pp 1601ndash1609 2013
[124] Y Fu L Du QWang et al ldquoIn vitro sustained release of recom-binant human bone morphogenetic protein-2 microspheresembedded in thermosensitive hydrogelsrdquo Die Pharmazie vol67 no 4 pp 299ndash303 2012
[125] D H R Kempen L Lu T E Hefferan et al ldquoRetention ofin vitro and in vivo BMP-2 bioactivities in sustained deliveryvehicles for bone tissue engineeringrdquo Biomaterials vol 29 no22 pp 3245ndash3252 2008
[126] D H R Kempen L Lu A Heijink et al ldquoEffect of localsequential VEGF andBMP-2 delivery on ectopic and orthotopicbone regenerationrdquo Biomaterials vol 30 no 14 pp 2816ndash28252009
[127] H Nie M-L Ho C-K Wang C-H Wang and Y-C FuldquoBMP-2 plasmid loaded PLGAHAp composite scaffolds fortreatment of bone defects in nude micerdquo Biomaterials vol 30no 5 pp 892ndash901 2009
[128] F Wegman Y van der Helm F C Oner W J A Dhert andJ Alblas ldquoBone morphogenetic protein-2 plasmid DNA as asubstitute for bone morphogenetic protein-2 protein in bonetissue engineeringrdquo Tissue Engineering Part A vol 19 no 23-24pp 2686ndash2692 2013
[129] J Fischer A Kolk S Wolfart et al ldquoFuture of local boneregenerationmdashprotein versus gene therapyrdquo Journal of Cranio-Maxillofacial Surgery vol 39 no 1 pp 54ndash64 2011
[130] C Qiao K Zhang H Jin et al ldquoUsing poly(lactic-co-glycolicacid) microspheres to encapsulate plasmid of bone morpho-genetic protein 2polyethylenimine nanoparticles to promotebone formation in vitro and in vivordquo International Journal ofNanomedicine vol 8 pp 2985ndash2995 2013
[131] C H Evans ldquoGene delivery to bonerdquo Advanced Drug DeliveryReviews vol 64 no 12 pp 1331ndash1340 2012
[132] C-H Lu Y-H Chang S-Y Lin K-C Li andY-CHu ldquoRecentprogresses in gene delivery-based bone tissue engineeringrdquoBiotechnology Advances vol 31 no 8 pp 1695ndash1706 2013
[133] TNVo F K Kasper andAGMikos ldquoStrategies for controlleddelivery of growth factors and cells for bone regenerationrdquo
18 BioMed Research International
Advanced Drug Delivery Reviews vol 64 no 12 pp 1292ndash13092012
[134] W Ji H Wang J J J P van den Beucken et al ldquoLocal deliveryof small and large biomolecules in craniomaxillofacial bonerdquoAdvanced Drug Delivery Reviews vol 64 no 12 pp 1152ndash11642012
[135] V Luginbuehl L Meinel H P Merkle and B Gander ldquoLocal-ized delivery of growth factors for bone repairrdquo EuropeanJournal of Pharmaceutics and Biopharmaceutics vol 58 no 2pp 197ndash208 2004
[136] P Kocbek N Obermajer M Cegnar J Kos and J Kristl ldquoTar-geting cancer cells using PLGA nanoparticles surface modifiedwith monoclonal antibodyrdquo Journal of Controlled Release vol120 no 1-2 pp 18ndash26 2007
[137] J Cheng B A Teply I Sherifi et al ldquoFormulation of func-tionalized PLGA-PEG nanoparticles for in vivo targeted drugdeliveryrdquo Biomaterials vol 28 no 5 pp 869ndash876 2007
[138] C Romagnoli F DrsquoAsta andM L Brandi ldquoDrug delivery usingcomposite scaffolds in the context of bone tissue engineeringrdquoClinical Cases inMineral and BoneMetabolism vol 10 no 3 pp155ndash161 2013
[139] D Puppi X Zhang L Yang F Chiellini X Sun and EChiellini ldquoNanomicrofibrous polymeric constructs loadedwith bioactive agents and designed for tissue engineeringapplications a reviewrdquo Journal of Biomedical Materials ResearchPart B Applied Biomaterials vol 102 no 7 pp 1562ndash1579 2014
Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595
Contents lists available at ScienceDirect
Colloids and Surfaces B Biointerfaces
jo ur nal ho me p ag e wwwelsev ier com locate co lsur fb
Full Length Article
Dual delivery nanosystem for biomolecules Formulationcharacterization and in vitro release
Inmaculada Ortega-Oller a1 Teresa del Castillo-Santaella b1 Miguel Padial-Molina aPablo Galindo-Moreno a Ana Beleacuten Joacutedar-Reyes b Joseacute Manuel Peula-Garciacutea bclowast
a Department of Oral Surgery and Implant Dentistry University of Granada Granada Spainb Biocolloid and Fluid Physics Group Department of Applied Physics University of Granada 18071 Granada Spainc Department of Applied Physics II University of Malaga 29071 Malaga Spain
a r t i c l e i n f o
Article historyReceived 25 May 2017Received in revised form 18 July 2017Accepted 17 August 2017
KeywordsPLGANanoparticlesProtein encapsulationRelease
a b s t r a c t
Because of the biocompatible and biodegradable properties of poly (lactic-co-glycolic acid) (PLGA)nanoparticles (NPs) based on this polymer have been widely studied for drugbiomolecule delivery andlong-term sustained-release In this work two different formulation methods for lysozyme-loaded PLGANPs have been developed and optimized based on the double-emulsion (wateroilwater WOW) sol-vent evaporation technique They differ mainly in the phase in which the surfactant (Pluronicreg F68) isadded water (W-F68) and oil (O-F68) The colloidal properties of these systems (morphology by SEM andSTEM hydrodynamic size by DLS and NTA electrophoretic mobility temporal stability in different mediaprotein encapsulation release and bioactivity) have been analyzed The interaction surfactant-proteindepending on the formulation procedure has been characterized by surface tension and dilatational rhe-ology Finally cellular uptake by human mesenchymal stromal cells and cytotoxicity for both systemshave been analyzed
Spherical hard NPs are made by the two methods However in one case they are monodisperse withdiameters of around 120 nm (O-F68) and in the other case a polydisperse system of NPs with diametersbetween 100 and 500 nm is found (W-F68) Protein encapsulation efficiency release and bioactivity aremaintained better by the W-F68 formulation method This multimodal system is found to be a promisingldquodual deliveryrdquo system for encapsulating hydrophilic proteins with strong biological activity at the cell-surface and cytoplasmic levels
copy 2017 Elsevier BV All rights reserved
1 Introduction
Tissue regeneration is a complex biological action involvingmultiple steps in a sequential ordered and controlled manner [12]Classically bioactive molecules have been proposed to aid in theseprocesses However the use of high doses denaturation and lossof biological activity uncontrolled timing of action and diffusionto other tissues have been highlighted as major issues of this ther-apeutic strategy [3] To help solve these problems nanomedicinehas been intensively investigated in recent years as an emerging
lowast Corresponding author at Department of Applied Physics II University of Maacutelaga29071 Maacutelaga Spain
E-mail address jmpeulaumaes (JM Peula-Garciacutea)1 Both authors contributed equally to this work
area This involves diagnostic therapeutic and regeneration meth-ods by means of structures and systems in which size and shape arecontrolled at the atomic molecular and supramolecular levels [4]The transport and controlled delivery of drugs andor therapeuticbiomolecules improve their pharmacokinetics and pharmacody-namics and at the same time minimize harmful side effects Forthese purposes different nanosystems have been described Polylactic-co-glycolic acid (PLGA) exhibits low cytotoxicity as well ashigh biocompatibility and biodegradability with the release of non-toxic by-products [5]
In the last decade the use of PLGA has been investigated todeliver a wide spectrum of active agents from hydrophobic drugmolecules [6ndash8] to hydrophilic biomolecules as peptides [9] pro-teins [10ndash15] or nucleic acids [1617] These delivery systemshave been produced via different formulation processes for theirapplication in both systemic and local site-specific therapies [18]
httpdxdoiorg101016jcolsurfb2017080270927-7765copy 2017 Elsevier BV All rights reserved
I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595 587
However their design and development as nanocarriers are diffi-cult due to the problematic release pattern when the encapsulatedmolecules are proteins for which initial bursts and slow or incom-plete release might be a problem [18ndash20] Moreover the specificconditions of the release may need to be different depending onthe final application of the nanocarrier [2021]
The water-in-oil-in-water (WOW) double emulsion techniqueis the most widely used protein-encapsulation method for PLGAmicro- (MP) and nanoparticles (NP) [2223] It allows differentfactors to be modulated such as the type of PLGA the use ofother polymers blended with PLGA the addition of surfactants themechanical stress or the organic solvent [20] It is also possible toconstruct several types of co-polymers to modify the hydropho-bicityhydrophilicity ratio [1824] and the colloidal stability sizeand release process PLGApolyethylene glycol pair and surfactantssuch as polyvinyl alcohol (PVA) or polyethylene oxides (PEO) arethe most widely studied [7122526]
On the other hand tissue engineering requires the participa-tion of mesenchymal stromal cells (MSCs) [27] MSCs are known tohave the ability to differentiate into multiple cell types includingosteoblasts Osteoblasts are the main cells responsible for synthe-sizing the mineralized compartment of bone tissue This processis regulated by among other molecules BMP-2 [3] PLGA parti-cles loaded with BMP-2 have been extensively used as has beendescribed and reviewed elsewhere [328ndash31]
Thus within this context it was the aim of the current study tooptimize the formulation and properties of a nanoparticle systemwith potential therapeutic applications Two different strategies toobtain PLGA-surfactant NPs were tested by using lysozyme as amodel for BMP-2 The size and morphology polydispersity indexzeta potential colloidal stability and encapsulation efficiency (EE)of the protein were analyzed
Once the physico-chemical characterization was completed thestudy was focused on the protein-release process using differ-ent techniques to study the results of in vitro experiments andfocusing it on the release pattern and the biological activity of thelysozyme released In this way a new formulation was establishedto develop a PLGA nanosystem with a singular dual size distribu-tion and the adequate balance between encapsulation and releaseof biologically active proteins Finally the effects of the proposedPLGA system were tested on primary MSCs in vitro as a proof ofconcept
2 Materials and methods
21 Formulation of the nanoparticles
Poly(lactide-co-glycolide) acid (PLGA 5050) ([C2 H2 O2]x [C3H4 O2]y) x = 50 y = 50 (Resomerreg 503H) 32ndash44 kDa was used asthe polymer The polymeric surfactant Pluronicreg F68 (Poloxamer188) (Sigma-Aldrich) was used as the emulsifier The structureis based on a poly(ethylene oxide)-block-poly(propylene oxide)-block-poly(ethylene oxide) and it is expressed as PEOa-PPOb-PEOawith a = 75 and b = 30 Lysozyme from chicken egg white (Sigma-L7651) was used as hydrophilic protein Water was purified ina Milli-Q Academic Millipore system Two different formulationmethods were developed termed O-F68 and W-F68
In the O-F68 method 25 mg of PLGA and 15 mg of F68 were dis-solved in 660 L of dichloromethane (DMC) and vortexed Then330 L of acetone were added and vortexed Next 100 L of abuffered solution at pH 128 with or without lysozyme (5 mgmL)were added dropwise while vortexing for 30 s Immediately thisprimary wateroil (WO) emulsion was poured into a glass con-taining 125 mL of ethanol under magnetic stirring and 125 mLof MilliQ water were added After 10 min of magnetic stirring the
organic solvents were rapidly extracted by evaporation under vac-uum until the sample reached a final volume of 10 mL
In the W-F68 method 100 mg of PLGA were dissolved in a tubecontaining 1 mL of ethyl acetate (EA) and vortexed 40 L of abuffered solution at pH 128 with or without lysozyme (20 mgmL)were added and immediately sonicated (Branson Ultrasonics 450Analog Sonifier) fixing the Duty cycle dial at 20 and the Outputcontrol dial at 4 for 1 min with the tube surrounded by ice This pri-mary WO emulsion was poured into a plastic tube containing 2 mLof a buffered solution (pH 128) of F68 at 1 mgmL and vortexingfor 30 s Then the tube surrounded by ice was sonicated again atthe maximum amplitude for the micro tip (Output control 7) for1 min This second WOW emulsion was poured into a glass con-taining 10 mL of the buffered F68 solution and kept under magneticstirring for 2 min The organic solvent was then rapidly extractedby evaporation under vacuum to a final volume of 8 mL
22 Cleaning and storage
After the organic solvent evaporation the sample was cen-trifuged for 10 min at 20 C at 14000 or 12000 rpm for O-F68 andW-F68 methods respectively The supernatant was filtered using100 nm filters for measuring the free non-encapsulated protein Thepellet was then resuspended in PB up to a final volume of 4 mL andkept under refrigeration at 4 C
221 Protein loading and encapsulation efficiencyThe initial protein loading was optimized for the nanoparticle
formulation preserving the final colloidal stability after the evapo-ration step and being different for each nanosystem Also 16 ww(LysPLGA) was used for O-F68 and 08 ww (LysPLGA) for W-F68one The amount of encapsulated lysozyme was calculated by mea-suring the difference between the initial amount added and the freenon-encapsulated protein which was tested by bicinchoninic acidassay (BCA Sigma-Aldrich) Then protein encapsulation efficiency(EE) and final drug loading (DL) was calculated as follows
EE = MI minus MF
MItimes 100 DL = MI minus MF
Mpolymertimes 100
where MI the initial total mass of Lys MF is the total mass of Lys inthe aqueous supernatant and Mpolymer is the mass of PLGA in theformulation
23 Characterization of the nanoparticles
231 Interfacial characterization of the first water-in-oilemulsion
The surface tension and dilatational rheology measurementsat the air-water interface were made in the OCTOPUS [32] aPendant Drop Surface Film Balance equipped with a subphasemulti-exchange device (patent submitted P201001588) describedin detail elsewhere [33] Here air plays the role of the organic phaseThe surface tension is calculated with DINATENreg software basedon axisymmetric drop shape analysis (ADSA) and the dilatationalmodulus (E) of the interfacial layer is determined from image anal-ysis with the program CONTACTOreg The in vitro model is describedin ldquoSupplementary materialrdquo
232 Particle morphologyNanoparticles were imaged by Scanning Electron Microscopy
(SEM) and Scanning Transmission Electron Microscopy (STEM)using a Zeiss SUPRA 40VP field emission scanning electron micro-scope from the Centre for Scientific Instrumentation of theUniversity of Granada (CIC UGR)
588 I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595
233 Nanoparticle size and electrokinetic mobilityThe hydrodynamic diameter and electrophoretic mobility of the
NPs were determined by using a Zetasizer NanoZeta ZS device(Malvern Instrument Ltd UK) working at 25 C with a He-Ne laserof 633 nm and a scattering angle of 173 Each data point wastaken as an average over three independent sample measurementsThe size of the NPs was characterized by Dynamic Light Scattering(DLS) The average hydrodynamic diameter (Z-average or cumu-lant mean) and the polydispersity index (PDI) were computedThese parameters are calculated through a cumulant analysis ofthe data which is applicable for narrow monomodal size distribu-tions [34] We also determined the intensity size distribution froman algorithm provided by the Zetasizer software (General Purpose)
The electrophoretic mobility was determined by the techniqueof Laser Doppler Electrophoresis An electrophoretic mobility dis-tribution as well as an average electrophoretic mobility (-average)was established for each sample
The hydrodynamic size distribution of the NPs with wide sizedistributions from DLS was also measured by using Nanoparti-cle Tracking Analysis (NTA) in a NanoSight LM10-HS(GB) FT14(NanoSight Amesbury United Kingdom) All samples were mea-sured more than three times for 60 s with manual shutter gainbrightness and threshold adjustments at 25 C The average sizedistribution (particle concentration vs diameter) was calculatedas an average of at least three independent size distributions
234 Nuclear magnetic resonance (NMR) of the nanoparticlesThe 1HNMR spectra of free F68 lysozyme-loaded particles from
O-F68 method with and without F68 and lysozyme-loaded parti-cles from W-F68 method were measured with a VNMRS 500 MHzspectrometer (Agilent) in the Centre for Scientific Instrumentation(CIC) of the University of Granada
24 Colloidal and temporal stability in biological media
The average hydrodynamic diameter and the polydispersityindex (PDI) by DLS of each system were measured to determinetheir colloidal stability in different media (Phosphate buffer [PB]Phosphate buffer saline [PBS] and cell culture medium Dulbeccorsquosmodified Eaglersquos medium [DMEM] from Sigma) and at differenttimes after (0 1 and 5 days)
In vitro release experiments were conducted following a simi-lar methodology as described above (Encapsulation efficiency) butusing 1 mL of each sample suspended in PBS at 37 C The proteinreleased from these samples was determined every 24 h by super-natant analysis and the pellet was suspended in the same volumeof buffer to maintain the release conditions All experiments weredeveloped in triplicate
241 Confocal microscopyLysozyme was labeled with fluorescein isothiocyanate (FITC)
using a method described by Kok et al [35] After FITC and lysozymecovalent conjugation concentrations were estimated spectropho-tometrically using the extinction coefficients described for FITC at494 nm and 280 nm The lysozyme concentration was calculatedmeasuring optical absorbance at 280 nm and subtracting the cor-responding FITC absorbance at this wavelength Images were madein a Nikon A1 laser scanning confocal microscope from CIC UGRAll experiments were performed in triplicate and replicated at leasttwice
25 Biological activity and interactions
251 Lysozyme biological activityThe biological activity of lysozyme was analyzed by an enzy-
matic activity kit (Sigma-Aldrich) using Micrococcus lysodeikticuscells as the substrate following the manufacturerrsquos instructions
252 Cellular uptakePrimary human mesenchymal stem cells (hMSCs) were taken
from healthy maxillary alveolar bone according to previouslydescribed protocols [36] After confirming their phenotype byflow cytometry and trilineage differentiation tests 12000 cellsper well were cultivated in sterile plates with glass bottom(Ibidi cat n 81158) overnight These cells were treated withmedium without fetal bovine serum (FBS) and Cell Tracker Red(15000) (C34552 ThermoFisher) for 30 min Then the mediumwas removed and supplemented with 10 FBS after which theparticles with lysozyme-FITC were added Then the hMSCs wereincubated 30 min again washed three times with PBS 1X and freshmedium supplemented with 2 FBS added Finally the hMSCs wereexamined by a confocal microscope (Nikon Eclipse Ti-E) Cell cul-tures were in all cases maintained at 37 C and 5 CO2 atmosphere
3 Results and discussion
31 Formulation of the nanoparticles
The methods developed in this work are intended to improvethe existing formulation techniques for hydrophilic protein loaded-PLGA NPs based on a double-emulsion process [1022] The noveltyof these methods is the use of the polymeric surfactant F68 either inthe organic phase (O-F68 method) or in the aqueous phase (W-F68)This surfactant reduces the size of the NPs enhances their stabilityand protects the encapsulated protein In addition the presence ofF68 on the surface of the particles reduces the recognition of thenanocarriers by the mononuclear phagocytic system (MPS) [37]
Additionally the choice of the organic solvent significantlyaffects the properties of the final colloidal system since the organicsolvent solubility regulates the inner and surface structure of theparticle In addition the interaction of the solvent with the encap-sulated biomolecule can alter its bioactivity as a consequence ofits denaturation as found for methylene chloride [26] In the O-F68method DMC is chosen as the organic solvent due to its lower watersolubility to facilitate the emulsification process and its low boil-ing point for easy evaporation However a freely water-miscibleorganic solvent (acetone) and the emulsifier F68 were added inthis organic phase to reduce its negative biological effects on theencapsulated protein [24] This emulsifier also reduces the protein-hydrophobic PLGA matrix interaction and thus the disruption ofthe protein structure [3] By contrast in the W-F68 method ethylacetate was used as the organic solvent which exerts less denatur-izing effects on the encapsulated protein [38] The higher watersolubility of this solvent favors rapid solvent removal The sol-vent removal rate is also accelerated by increasing the shear stressduring the second emulsification step It also enhances the encap-sulation efficiency and minimizes the contact time between theprotein and organic solvent [3] Poloxamer F68 is introduced in theexternal aqueous phase
Both formulations (O-F68 and W-F68) (Table 1) gave rise to col-loidally stable samples and the encapsulation of lysozyme insidethe nanoparticles in agreement with the double WOW emulsionmethod [23] Lysozyme was chosen as a model protein due to itsbiostability well-known characteristics and ease in quantifying itsbiological activity [3940] In addition its molecular size (143 kD)and its basic isoelectric point (around pH = 11) make it an appro-
I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595 589
Table 1Formulation conditions and protein encapsulation results PLGA F68 and LYSI are the initial amount of polymer surfactant and lysozyme respectively Initial is the initialpolymer-protein rate in ww EE is the encapsulation efficiency LYSF is the final encapsulated amount of lysozyme DL is the final drug loading rate in ww
PLGA (mg) F68 (mg) LYSI (mg) Initial EE LYSF (mg) DL
O-F68-Lys 25 15 04 16 625 025 1W-F68-Lys 100 2 08 08 731 058 058
priate model for other proteins such as bone-growth factors [15]Three main objectives drove the optimization of the appropriaterelation among the polymer poloxamer and protein (1) to havecolloidally stable nanosystems of submicron sizes (2) to encap-sulate a sufficient amount of protein and (3) to prevent proteindestabilization by maintaining their biological activity
Therefore regardless of the formulation method it wasintended to limit the initial protein loading to provide colloidallystable nanosystems In our case as shown in Table 1 Initial val-ues were the best choice to maintain colloidal stability withoutsignificantly changing the size distribution (see below) In con-sequence DL presents relative low values for both formulationsalthough the encapsulated amount of lysozyme LYSF is greaterthan those required for therapeutic proteins with lower clinicallyeffective amounts [41] The value of EE found for O-F68-Lys NPsis in consonance with the formulation characteristics and simi-lar to other reports with different proteins [12104214] includingbovine serum albumin (BSA) or insulin [1242] and several growthfactors [14]
The presence of surfactant stabilizes the emulsion droplets andreduces their size However it also alters the protein-polymerinteraction which translates into a reduction of the encapsulationefficiency This was evidenced by Blanco et al when encapsulat-ing BSA and lysozyme in different PLGA-poloxamer microparticles[10] Moreover the type of protein and its initial theoretical loadingare factors directly related with the EE and can affect the colloidalstability of the primary emulsion as shown by Santander et al [12]The different polymersurfactant ratio between the two formula-tions is not comparable since the surfactant is added in a differentway In both cases we used previous formulations as the startingpoint [1022] and tested several polymersurfactant ratios (datanot shown) in order to obtain the best colloidal stability EE andDL In Table 1 we show the data for the optimized PLGAF68 ratiosin both systems
In the W-F68 method despite the higher EE value with respectto O-F68 system an almost complete encapsulation was expecteddue to the low initial proteinPLGA mass ratio [12] and to theabsence of surfactant in the first emulsion step The characteris-tics of the modified formulation process may have the key In thisformulation the relatively high solubility of the ethyl acetate inwater promotes rapid diffusion of the organic solvent into the sec-ond aqueous phase An initial small volume of water containingpoloxamer is initially added to prevent a rapid uncontrolled precip-itation of the polymer and to control the speed of the process Thisis subsequently supplemented with the addition of a larger aque-ous volume as previously described [26] When this solidificationis slow it favors the escape of the protein and the EE decreasesHowever if the solidification is very fast the contact of proteinwith the organic solvent is minimized and the EE increases On thenegative side it can produce polymer agglomeration which inter-feres with the correct formation of the NPs The introduction of anintermediate step with a reduced volume of aqueous phase withpoloxamer can modulate the rate of the process by controlling thediffusion of ethyl acetate into the water and by allowing the diffu-sion into the organic phase of the poloxamer A controlled velocityof the polymer pre-solidification process in the presence of surfac-tant can produce channels or pores in the polymeric shell that onone hand could facilitate the protein release and on the other hand
could drive down the EE value [43] As a result of these phenom-ena the final DLs (ww of lysozymepolymer) shown in Table 1 forboth NP systems are suitable for their application as nanotransportsystems
32 Characterization of the nanoparticles
321 Interfacial characterization of the first water-in- oilemulsion
To gain better insight into the effect of the formulation methodon the interfacial properties of the first water (lysozyme solution)-in-oil emulsion we designed surface experiments with lysozymeand Pluronicreg F68 The main difference in the two formulationmethods is how the Pluronicreg F68 is added in aqueous phase(WndashF68) or in organic phase (O-F68) This difference could affectthe composition of the surface of the NPs and as a result theircolloidal properties
The surface tension and elasticity at the air-water interfacewere the properties analyzed (Table 2) At this interface proteinschange their conformation and expose their hydrophobic part toair depending on their thermodynamic stability flexibility amphi-pathicity molecular size and charge In our case lysozyme is aglobular protein that is adsorbed at the air-water interface andforms a rigid monolayer due to its internal structure and the pres-ence and number of disulfide bridges [44] Our measurements weremade at pH 12 thus lysozyme is negatively charged Table 2 showsthe interfacial tension of the lysozyme monolayer at the air-waterinterface after 50 min of adsorption (457 plusmn 04 (mNm)) and itselasticity (83 plusmn 4 (mNm)) The reduction of the interfacial tensionwhen compared with that of the air-water interface (72 mNm)indicates the surfactant characteristics of the lysozyme The highvalue of elasticity was due to the charge and high molecular inter-actions in the lysozyme monolayer When the monolayer is formedwith Pluronicreg F68 the surface tension is slightly lower than withlysozyme when the Pluronicreg is added in AP but similar (takinginto account the error) when added in OP
Pluronicreg F68 is an amphiphilic molecule that is adsorbed at theair-water interface when it is dissolved in aqueous phase and alsowhen it is deposited onto the surface of the drop Small differencesare found when comparing the surface tension of the Pluronicreg
monolayer from the two methods The different values of interfa-cial tension attained in both cases would be due to the differentmethods to add the Pluronicreg F68 at the formed lysozyme mono-layer Pluronicreg F68 presents lower elasticity than the lysozyme asexpected since Pluronicreg F68 is known to form a flexible monolayerat the air-water interface [45]
Two assays were designed to mimic the formulation methodsof the particles In the first assay (W-F68 method) a monolayer oflysozyme was formed then the bulk of the drop was exchangedwith the aqueous solution of Pluronicreg F68 and after adsorptionthe interfacial tension and elasticity of the interface were mea-sured (379 plusmn 06 mNm and 142 plusmn 05 mNm respectively) Thislow value of elasticity was very similar to that of the monolayerof Pluronicreg F68 indicating that Pluronicreg F68 is located at theinterface and removes the previously adsorbed lysozyme In thesecond assay (O-F68 method) after the monolayer of lysozyme wasformed the Pluronicreg F68 dissolved in chloroform is deposited ontothe surface of the drop The chloroform is rapidly evaporated and
590 I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595
Table 2Interfacial tension and dilatational elasticity (at 1 Hz) of the air-water interface (a) after adsorbing lysozyme or Pluronicreg F68 in the aqueous phase (AP) or Pluronicreg F68 inorganic phase (OP) in the first step (b) when Pluronicreg F68 is added in AP or OP after adsorption of lysozyme monolayer (mean plusmn sd n = 3)
First step Interfacial Tension(mNm)
Elasticitya
(mNm)Second step Interfacial Tension
(mNm)Elasticityb
(mNm)
Lysozyme 457 plusmn 04 83 plusmn 4 Pluronicreg F68 (AP) 379 plusmn 06 142 plusmn 05Lysozyme 457 plusmn 04 83 plusmn 4 Pluronicreg F68 (OP) 38 plusmn 2 43 plusmn 4Pluronicreg F68 (AP) 421 plusmn 03 15 plusmn 3Pluronicreg F68 (OP) 475 plusmn 21 94 plusmn 05
the interfacial tension and elasticity of the interface are measured(38 plusmn 2 mNm and 43 plusmn 4 mNm respectively) The elasticity washalf of that of the pure lysozyme monolayer perhaps because ofthe coexistence of lysozyme and Pluronicreg F68 molecules at theinterface The surface tension of the final interface does not dependon the method of adding the Pluronicreg but it is lower than that ofthe pure lysozyme or the pure Pluronicreg
Within this context it has been widely reported that the adsorp-tion of PEO and poloxamers at the interface reduces the proteinbinding [4647] In the O-F68 method the lysozyme is exposedto the DCM after the formation of the first water-in-oil emulsioneven if Pluronicreg is added as they both coexist at the interface Inthe W-F68 method protein will be in contact with ethyl acetate inthis step as Pluronicreg is absent However this solvent has weakerbiological effects on lysozyme Pluronicreg could reach the interfacewhen added to the aqueous phase in the following step and dis-place the protein from the interface which could diffuse outwardsto the aqueous phase
322 Particle morphologyThe delivery biodistribution and action mechanism of a trans-
ported drug or biomolecule depend heavily on the size of theparticle concentration and timing [48] In general the micromet-ric scale is designed for a local supply that allows the formation ofreservoirs of the transported molecule and minimizes the actionof the phagocytic system [49] However nanometric systems aremore versatile because they permit a systemic distribution aremore stable and reactive and allow extra- as well as intracellu-lar action This latter mechanism is essential when the moleculeor drug should act in the cytoplasm [50] or any other intracellularstructure such as the mitochondria Golgi apparatus endoplasmicreticulum or nucleus [485152] Other parameters to alter the intra-cellular fate of the particles have also been investigated mainly byaltering their surface decoration [53] for example with nuclearlocalization signals (NLS) that use the nucleus as the target of theparticle [51] However these strategies are still in their very earlydevelopmental phase [4852]
A particle size in the submicron scale (between 2 and 500 nm)was sought as it is necessary for cell internalization and a rapiddistribution after parenteral administration in order to reach dif-ferent tissues through different biological barriers Particles under200 nm minimize their intake by macrophages The type of organicsolvent the polymer concentration the addition of surfactant andthe emulsification energy control the size of the system
The O-F68 method gives rise to a monomodal particle-size dis-tribution with diameters around 100 nm The addition of Pluronicreg
F68 in the organic phase bolsters colloidal stability of the first emul-sion and reduces the particle size in comparison with PLGA NPsin which the stability is purely electrostatic due to the carboxylicgroups of the PLGA In the W-F68 method shear stress and volumeof the aqueous phase are taken into account to produce a systemwith particles of between 100 and 500 nm
O-F68-Lys NPs have a spherical shape with a monomodal sizedistribution (diameters around 100 nm) and core-shell structure(Fig 1a) Empty particles produced with the O-F68 method are
shown in Figs S1 (without F68) and S2 (with F68) They are alsospherical and with a core-shell structure but slightly larger
W-F68-Lys NPs also present a spherical shape but a multi-modal size distribution with diameters between 140 and 450 nmthe largest population being around 260 nm (Fig 1b) A core-shellstructure is also observed in these particles Empty particles fromthe W-F68 method are presented in Fig S3 corresponding to a morepolydisperse system
323 Nanoparticle size electrokinetic mobility and colloidalstability
The hydrodynamic diameter distribution of the particles wasdetermined firstly by DLS Table 3 contains the main colloidal prop-erties of particles produced with the O-F68 and W-F68 methodsempty or loaded with lysozyme The results of empty particles fromthe O-F68 method but synthesized without F68 are also included
The size parameters were calculated through a cumulative anal-ysis of the data which is applicable for narrow monomodal sizedistributions [34] SEM and STEM micrographs indicate that suchan approximation could be assumed for particles from the O-F68method but not from the W-F68 one Thus the intensity size distri-butions of the different systems are shown in Fig 2a The presenceof Pluronicreg F68 in the O-F68 method significantly reduces the sizeand polydispersity of the NPs This agrees with the reduction of thesurface tension when the F68 is at the interface (Table 2) whichpromotes the emulsification process If the NPs are also loaded withlysozyme the size is even smaller but the polydispersity increasesslightly compared with the empty particles The surfactant prop-erties of the lysozyme have been shown with the surface-tensionresults (Table 2)
Fig 2a indicates the presence of particles higher than 500 nmwith the W-F68 which does not correlate with the SEM micro-graphs Thus a different technique (NTA) was used to gaininformation on the size distribution of such systems (Fig 3b) WithNTA the size distribution was consistent with the SEM imagesBroad size distributions corresponding to multimodal systemswere found with this method but the addition of lysozyme ledto a clear size reduction This is because lysozyme also acts as anemulsifier in the first emulsion
The electrokinetic charge of the NPs was analyzed by measuringthe electrophoretic mobility For comparison all the samples weremeasured at pH 7 (phosphate buffer) In Fig 3 the electrophoreticmobility distributions are presented while the corresponding -averages are shown in Table 3
PLGA NPs are usually negatively charged due to the carboxylicgroups of the polymer The use of Pluronicreg F68 in the O-F68method clearly reduces the electrophoretic mobility of the NPswhich indicates that some Pluronicreg is located at the NP sur-face This reduction was expected after the incorporation of thisnon-ionic surfactant onto the interface since the presence ofpolyethylene oxide chains would cause an outward shift of theshear plane where the -potential is defined and this would sub-sequently diminish electrophoretic mobility Previous results forPLGA particles have shown a significant reduction directly relatedto the poloxamer coating [54] If we compare the two systems the
I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595 591
Fig 1 SEM and STEM micrographs of lysozyme-loaded particles using O-F68 (a) or W-F68 method (b)
Table 3Colloidal properties of PLGA NPs from different formulation methods They were measured in phosphate buffer (pH 7) The average hydrodynamic diameter (Z-average orcumulative mean) and the polydispersity index (PDI) are determined from DLS (Mean plusmn sd n = 3)
Z-average (nm) PDI -average (mcmVs)
O-F68 method Empty without F68 266 plusmn 7 0293 minus506 plusmn 015Empty 1627 plusmn 21 0081 minus429 plusmn 018Lysozyme-loaded 1210 plusmn 12 0244 minus334 plusmn 007
W-F68 method Empty 273 plusmn 3 0193 minus531 plusmn 011Lysozyme-loaded 293 plusmn 4 0169 minus4212 plusmn 0013
Fig 2 Hydrodynamic diameter distribution (a) by DLS at pH 7 (phosphate buffer) of empty and lysozyme-loaded PLGA particles from the O-F68 and W-F68 methods and(b) by NTA at pH 7 (phosphate buffer) of empty and lysozyme-loaded PLGA particles from the W-F68 method
less negative surface for OF68 NPs would be related to less densityof surface PLGA polymer bringing the negative electrical charge tothe interface This result would be in line with the greater amountof PLGA in the formulation of WF68 nanosystem
When the lysozyme is also used in the synthesis the surfaceis even less negative which could be explained by the presence
of some protein (whose net charge is positive) near or at theinterface This latter effect is also found with the W-F68 methodThe attractive electrostatic interaction between negative terminalacid residues of PLGA and lysozyme molecules plays a key rolein the process of protein encapsulation [41] or adsorption [40]in PLGA NPs which affects the final protein loading In relation
592 I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595
Fig 3 Electrophoretic mobility distribution at pH 7 (phosphate buffer) of empty and lysozyme-loaded PLGA particles from the (a) O-F68 and (b) W-F68 methods
to this situation an important characteristic of the W-F68 encap-sulation formulation is that the water phase is at pH 12 whichallows a negative net charge of lysozyme and thus avoids theelectrostatic protein-polymer attraction This situation can reducethe encapsulation efficiency but at the same time favors the laterprotein-diffusion process and consequently the short-term release
Recent studies have proposed the use of nanoparticles embed-ded in predesigned 3D-printed scaffolds [5556] moving us toanalyze the stability of the two formulations in several mediausually employed during the preparation of other structures Sizedistributions similar to the original were found for the two formula-tions in different media (PB PBS and DMEM) and at different timesafter synthesis (0 1 and 5 days) The electric charge of PLGA acidend groups and the poloxamer molecules located on the NP sur-face confers a combined electrostatic and steric colloidal-stabilitymechanism as has previously been described [4654] Additionallythe NPs in all cases keep their size under storage at 4 C at least for1 month (data not shown) Thus the media described could poten-tially be used as storage media or to prepare other solutions orscaffolds before actually placing them in the living environment(in vitro or in vivo)
324 NMR of the nanoparticlesIn Fig 3 both empty and protein-loaded NPs present less neg-
ative electrophoretic mobility than do empty NPs without F68which could be explained by the presence of Pluronicreg F68 atthe surface of the NP By comparing the 1HNMR spectra of freePluronicreg F68 and lysozyme-loaded NPs from O-F68 and W-F68methods we can check the presence of F68 at the surface of theNPs (Fig S4) by the peaks shown between 325 and 375 ppm and at1 ppm These peaks are also visible in the spectra of NPs formulatedwith F68 (O-F68 and W-F68 Figs S5 and S6 respectively)
33 Biological activity and interactions
A controlled release from a PLGA-based delivery system is adifficult task as it depends on multiple factors the type of PLGAsolvent mechanical stress use of surfactants etc [57] The diffusionof the protein and the polymer erosion are the main mechanismsinvolved in the protein release in PLGA-based delivery systems Fur-thermore it is typical to find a rapid burst release at the initial stagefollowed by a slow release phase over the short and medium termIn this phase protein molecules diffuse through the polymer matrixuntil reaching a final phase in which the polymer degradation byhydrolysis allows a faster release [20]
On the other hand the short-term release is of special inter-est for transporting bone morphogenetic growth factors (BMPs) Acontrolled initial burst followed by a sustained release significantlyimproves in vivo regeneration of bone [3] and cartilage [58] even in
Fig 4 Cumulative release (filled symbols) and residual bioactivity (open symbols)of O-F68-Lys (square) and W-F68-Lys (triangle) incubated for different times at 37 Cin saline phosphate buffer (pH 74) (mean plusmn sd n = 3)
dual-controlled release systems [59] For these reasons we focusedour analysis on short-term release taking into account the reducedpolymer degradation by hydrolysis found for similar systems forthese early steps [60]
Fig 4 shows the accumulative release of lysozyme from O-F68-Lys NPs over the short term (seven days) These results areconsistent with a two-stepped process an initial burst and a slow-release phase The first step could correspond to the release ofthe protein molecules located near surface whose presence wasdeduced from the electrophoretic mobility results (Fig 3) The sec-ond part of the release process was limited and slow due to theprotein diffusion through the matrix of the polymeric shell Thespecific electrostatic interaction between the positive lysozymemolecules and the PLGA negative terminal acid groups can reducethe protein diffusion [10] When the poloxamer (F68) is added theinteraction between the surfactant and the protein helps the diffu-sion process leading to a more complete and sustained release [12]It also helps to keep the biological activity of the protein [4161] Thepoloxamer reduces the non-specific protein-polymer interactions(ie hydrophobic interactions) but not the specific ones (electro-statics) thus the diffusion through water-filled pores or throughthe polymer is still limited In the current study the protein fractionreleased and the release pattern are similar to those found in theliterature for lysozyme encapsulated in nano- and microparticlesof blends of PLGA and other polymers or surfactants [261511]
The protein release curve from W-F68-Lys NPs (Fig 4) revealsthat the initial delivery rate is identical to that of the O-F68 systemwhich could mean a similar proportion of encapsulated proteinclose to or at the surface for both NP systems This would agreewith the analogous decrease in the electrophoretic mobility of the
I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595 593
Fig 5 z-projection of 5 images of hMSCs visualized 30 min after incubation with W-F68-LysFITC NPs or O-F68-LysFITC NPs hMSCs were previously labeled with cell-trackerred Scale bar 20 m
lysozyme-loaded NPs previously reported (Fig 3) In the secondpart of the process the specific interaction between the proteinand the polymer is again present However the diffusion processin the W-F68 system appears to be enhanced allowing a contin-uous and sustained release after the initial burst and reaching aslightly higher value for the maximum release time studied Thisresult could be related to the inner structure of the polymer layerthat allows better hydration and therefore better diffusion of theprotein towards the outside It has been previously reported thatthe use of less polar organic solvents such as DCM for PLGA par-ticles formulations increases the density of the polymer matrix incomparison with more polar organic solvents such as EA The PLGAmatrices prove more resistant in the first case but reducing at thesame time their connectivity and diffusivity [62] Meng et al [26]found that faster removal of EA results in a slower kinetic release ofthe protein due to a decrease in the porosity of the NPs Regardingthe role of the Pluronicreg Rafati et al [63] found a higher concentra-tion of protein encapsulated in the surface pores in microparticlessynthesized in the presence of surfactant in the second aqueousphase of the emulsion Since an intermediate step was introducedin our W-F68 formulation in the second aqueous phase of the emul-sion the removal of the EA by diffusion was strongly controlled sothat it was expected that the porosity of these NPs would increaseThis porosity improves protein diffusion which allows a more sta-ble release pattern according to the experimental result found forthis system Despite the unfavorable effect of the specific electro-static protein-polymer interaction on the release the amount ofreleased protein in our NPs is substantial signifying that there areother unspecific interactions that can be modulated by the pres-ence of surfactant allowing a sustained release The amount ofreleased lysozyme is similar to that found with lysozyme physi-cally adsorbed onto the surface of PLGA nanoparticles despite theelectrostatic attraction [40] Besides other unspecific interactionsthe electrolyte concentration in the release medium could modu-late this electrostatic attraction between the protein and polymerdiminishing it and facilitating the release process [46]
Another remarkable parameter is the biological activity of thein vitro release of lysozyme shown in Fig 4 While in the O-F68system the bioactivity is partially reduced by up to 40 the pro-
tein supplied by the W-F68 system maintains the activity above90 with respect to that of commercially supplied lysozyme andresuspended in the same release buffer As discussed above boththe organic solvent and the hydrophobic interaction between theprotein and the polymer often cause denaturation of encapsulatedproteins [4164] Perez et al [11] describe a partial loss of activ-ity when using DCM and an aqueous PVA solution in the secondemulsification step without any additional excipient The use ofpoloxamers in the formulation reduces such interactions enhancesthe stability of the protein and maintains an aqueous layer thatretains the water molecules necessary for the biological functionof the protein at the same time aiding its diffusion This situationtogether with the use of a weak organic solvent such as EA helpspreserve the biological activity of the lysozyme as found for theW-F68-Lys system
Fig S7 presents different confocal microscopy images relatedto the release process of lysozyme-loaded W-F68 NPs A decreasein fluorescence intensity was appreciable over the course of thein vitro experiment In addition the aggregation of the system isvisible as the incubation process progresses The analysis of theseimages is consistent with the previously reported results for thisNP system
331 Cellular uptakeCellular uptake of PLGA NPs is a known process affected
mainly by surface properties and functionalization [9] and parti-cle aggregation [65] Internalization and subsequent intracellularprocessing of the particles have been described as an activeprocess thus it is energy dependent and can therefore beaffected by other factors that alter the energy uptake by cellssuch as temperature [48] Particles can be internalized by sev-eral endocytosis methods dependent primarily on the size ofthe particle caveolin-dependent particles (diameter asymp 60 nm)clathrin-independent (diameter asymp 90 nm) and clathrin-dependent(diameter asymp 120 nm) [5152] Once internalized about 65 areexported back to the extracellular space before releasing anyof their content while the rest slowly release the encapsulatedmolecule into the intracellular space [66] The intracellular releaseprocess is affected by the formulation of the particles [48] We have
594 I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595
demonstrated that the proposed systems follow a pattern similar toothers previously published As early as 30 min after incubation W-F68-LysFITC NPs were taken up by the cells (Fig 5) Some W-F68particles were still in the medium so that the dual activity couldhappen In contrast O-F68-LysFITC NPs were affected by aggrega-tion and therefore did not properly reach the intracellular space(Fig 5 for z-axis images view Fig S8) This contradicts the previousanalyses of the colloidal stability in PB PBS and DMEM This findingcan be explained by the fact that although the culture media wasDMEM this latter medium was supplemented with fetal bovineserum and cells release many factors to the extracellular mediumthat can affect these types of particles None of the systems wereshown to be toxic for the cells (Fig S9) No studies available havereported any effects of lysozyme on hMSCs
4 Conclusions
A novel dual-delivery PLGA-nanosystem has been developedin which the formulation and components favor an adequateshort-term delivery pattern while preserving the bioactivity ofencapsulated molecules The analysis of the polymer-surfactant-protein interaction shows that the organic solvent use ofsurfactant volume relation of both phases and the net charge of theprotein play important roles in the final characteristics and releasebehavior of the nanoparticles The W-F68 formulation balances allof them in order to provide a nanosystem ready to transport anddeliver hydrophilic biomolecules such as proteins In vitro releaseexperiments display an adequate short-term delivery pattern thatat the same time preserves the bioactivity of the encapsulatedbiomolecule Additionally the singular nanoparticle size distribu-tion found for this W-F68 nanosystem allows the possibility of adual outer- and intra-cellular protein delivery as has been shownby in vitro cellular experiments This novel formulation will be usedin future studies to encapsulate and deliver growth factors in vitroand in vivo in order to exploit the therapeutic potential of thisnanosystem
Acknowledgements
The authors wish to express their appreciation for the tech-nical support to Dr Azahara Rata-Aguilar and for the financialsupport granted by the Consejeriacutea de Economiacutea Innovacioacuten Cienciay Empleo de la Junta de Andaluciacutea (Spain) through research groupsFQM-115 and CTS-1028 and by the following research projectMAT2013-43922-R ndash European FEDER support included minus (MICINNSpain)
Appendix A Supplementary data
Supplementary data associated with this article can be found inthe online version at httpdxdoiorg101016jcolsurfb201708027
References
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[3] Bone regeneration from PLGA micro-Nanoparticles Biomed Res Int (2015)httpwwwhindawicomjournalsbmriaa415289
[4] K-B Lee A Solanki J Kim J Jung Nanomedicine dynamic integration ofnanotechnology with biomedical science in Handb Clin Nanomedicine PanStanford 2016 2017 pp 21ndash60 httpdxdoiorg101201b19915-4
[5] GEJ Poinern A Laboratory Course in Nanoscience and Nanotechnology CRCPress Taylor amp Francis Group 2015
[6] MM Yallapu BK Gupta M Jaggi SC Chauhan Fabrication of curcuminencapsulated PLGA nanoparticles for improved therapeutic effects inmetastatic cancer cells J Colloid Interface Sci 351 (2010) 19ndash29 httpdxdoiorg101016jjcis201005022
[7] BP Nair CP Sharma Poly(lactide-co-glycolide)-laponite-F68 nanocompositevesicles through a single-step double-emulsion method for the controlledrelease of doxorubicin Langmuir 28 (2012) 4559ndash4564 httpdxdoiorg101021la300005c
[8] R Shankarayan S Kumar P Mishra Differential permeation ofpiroxicam-loaded PLGA micronanoparticles and their in vitro enhancementJ Nanopart Res 15 (2013) 1496 httpdxdoiorg101007s11051-013-1496-6
[9] JA Loureiro B Gomes G Fricker MAN Coelho S Rocha MC PereiraCellular uptake of PLGA nanoparticles targeted with anti-amyloid andanti-transferrin receptor antibodies for Alzheimerrsquos disease treatmentColloids Surf B Biointerfaces 145 (2016) 8ndash13 httpdxdoiorg101016jcolsurfb201604041
[10] D Blanco MJ Alonso Protein encapsulation and release frompoly(lactide-co-glycolide) microspheres effect of the protein and polymerproperties and of the co- encapsulation of surfactants Eur J PharmBiopharm 45 (1998) 285ndash294 httpdxdoiorg101016S0939-6411(98)00011-3
[11] C Peacuterez P De Jesuacutes K Griebenow Preservation of lysozyme structure andfunction upon encapsulation and release from poly(lactic-co-glycolic) acidmicrospheres prepared by the water-in-oil-in-water method Int J Pharm248 (2002) 193ndash206 httpdxdoiorg101016S0378-5173(02)00435-0
[12] MJ Santander-Ortega N Csaba L Gonzaacutelez D Bastos-Gonzaacutelez JLOrtega-Vinuesa MJ Alonso Protein-loaded PLGAndashPEO blend nanoparticlesencapsulation release and degradation characteristics Colloid Polym Sci288 (2010) 141ndash150 httpdxdoiorg101007s00396-009-2131-z
[13] N Pirooznia S Hasannia A Lotfi M Ghanei Encapsulation of alpha-1antitrypsin in PLGA nanoparticles in Vitro characterization as an effectiveaerosol formulation in pulmonary diseases J Nanobiotechnol 10 (2012) 20httpdxdoiorg1011861477-3155-10-20
[14] I DrsquoAngelo M Garcia-Fuentes Y Parajoacute A Welle T Vaacutentus A Horvaacuteth GBoumlkoumlnyi G Keacuteri MJ Alonso Nanoparticles based on PLGA poloxamer blendsfor the delivery of proangiogenic growth factors Mol Pharm 7 (2010)1724ndash1733 httpdxdoiorg101021mp1001262
[15] LJ White GTS Kirby HC Cox R Qodratnama O Qutachi FRAJ Rose KMShakesheff Accelerating protein release from microparticles for regenerativemedicine applications Mater Sci Eng C 23 (2013) 2578ndash2583 httpdxdoiorg101016jmsec201302020
[16] P Pantazis K Dimas JH Wyche S Anant CW Houchen J Panyam RPRamanujam Preparation of siRNA-Encapsulated PLGA nanoparticles forsustained release of siRNA and evaluation of encapsulation efficiencyNanopart Biol Med (2012) 311ndash319 httpdxdoiorg101007978-1-61779-953-2 25 Humana Press Totowa NJ
[17] JS Park HN Yang DG Woo SY Jeon KH Park Multilineage differentiationof human-derived dermal fibroblasts transfected with genes coated on PLGAnanoparticles plus growth factors Biomaterials 34 (2013) 582ndash597 httpdxdoiorg101016jbiomaterials201210001
[18] F Wan M Yang Design of PLGA-based depot delivery systems forbiopharmaceuticals prepared by spray drying Int J Pharm 498 (2016)82ndash95 httpdxdoiorg101016jijpharm201512025
[19] A Giteau MC Venier-Julienne A Aubert-Poueumlssel JP Benoit How to achievesustained and complete protein release from PLGA-based microparticles IntJ Pharm 350 (2008) 14ndash26 httpdxdoiorg101016jijpharm200711012
[20] S Fredenberg M Wahlgren M Reslow A Axelsson The mechanisms of drugrelease in poly(lactic-co-glycolic acid)-based drug delivery systemsmdashareview Int J Pharm 415 (2011) 34ndash52 httpdxdoiorg101016jijpharm201105049
[21] F Mohamed CF van der Walle Engineering biodegradable polyesterparticles with specific drug targeting and drug release properties J PharmSci 97 (2008) 71ndash87 httpdxdoiorg101002jps21082
[22] N Csaba L Gonzaacutelez A Saacutenchez MJ Alonso Design and characterisation ofnew nanoparticulate polymer blends for drug delivery J Biomater Sci PolymEd 15 (2004) 1137ndash1151 httpdxdoiorg1011631568562041753098
[23] HK Makadia SJ Siegel Poly lactic-co-glycolic acid (PLGA) as biodegradablecontrolled drug delivery carrier Polymers (Basel) 3 (2011) 1377ndash1397 httpdxdoiorg103390polym3031377
[24] F Danhier E Ansorena JM Silva R Coco A Le Breton V Preacuteat PLGA-basednanoparticles an overview of biomedical applications J Controlled Release161 (2012) 505ndash522 httpdxdoiorg101016jjconrel201201043
[25] G Ratzinger U Laumlnger L Neutsch F Pittner M Wirth F Gabor Surfacemodification of PLGA particles the interplay between stabilizer ligand sizeand hydrophobic interactions Langmuir 26 (2010) 1855ndash1859 httpdxdoiorg101021la902602z
[26] FT Meng GH Ma W Qiu ZG Su WOW double emulsion technique usingethyl acetate as organic solvent effects of its diffusion rate on thecharacteristics of microparticles J Controlled Release 91 (2003) 407ndash416httpdxdoiorg101016S0168-3659(03)00273-6
[27] M Padial-Molina F OrsquoValle A Lanis F Mesa DM Dohan Ehrenfest H-LWang P Galindo-Moreno Clinical application of mesenchymal stem cells andnovel supportive therapies for oral bone regeneration Biomed Res Int 2015(2015) httpdxdoiorg1011552015341327
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[28] P Yilgor N Hasirci V Hasirci Sequential BMP-2BMP-7 delivery frompolyester nanocapsules J Biomed Mater Res ndash Part A 93 (2010) 528ndash536httpdxdoiorg101002jbma32520
[29] B Li T Yoshii AE Hafeman JS Nyman JC Wenke SA Guelcher The effectsof rhBMP-2 released from biodegradable polyurethanemicrospherecomposite scaffolds on new bone formation in rat femora Biomaterials 30(2009) 6768ndash6779 httpdxdoiorg101016jbiomaterials200908038
[30] Y Wang Y Wei X Zhang M Xu F Liu Q Ma Q Cai X Deng PLGAPDLLAcore-shell submicron spheres sequential release system preparationcharacterization and promotion of bone regeneration in vitro and in vivoChem Eng J 273 (2015) 490ndash501 httpdxdoiorg101016jcej201503068
[31] YB Shim HH Jung JW Jang HS Yang H Bae JC Park B Choi SH LeeFabrication of hollow porous PLGA microspheres using sucrose for controlleddual delivery of dexamethasone and BMP2 J Ind Eng Chem 37 (2016)101ndash106 httpdxdoiorg101016jjiec201603014
[32] J Maldonado-Valderrama JAH Terriza A Torcello-Goacutemez MACabrerizo-Viacutelchez In vitro digestion of interfacial protein structures SoftMatter (2013) 1043ndash1053 httpdxdoiorg101039c2sm26843d
[33] M a Cabrerizo-Vilchez H a Wege J a Holgado-Terriza a W NeumannAxisymmetric drop shape analysis as penetration Langmuir balance Rev SciInstrum 70 (1999) 2438ndash2444 httpdxdoiorg10106311149773
[34] PA Hassan S Rana G Verma Making sense of brownian motion colloidcharacterization by dynamic light scattering Langmuir 31 (2015) 3ndash12httpdxdoiorg101021la501789z
[35] RJ Kok M Haas F Moolenaar D de Zeeuw DK Meijer Drug delivery to thekidneys and the bladder with the low molecular weight protein lysozymeRen Fail 20 (1998) 211ndash217
[36] S Mason SA Tarle W Osibin Y Kinfu D Kaigler Standardization and safetyof alveolar bone-derived stem cell isolation J Dent Res 93 (2014) 55ndash61httpdxdoiorg1011770022034513510530
[37] C Farace P Saacutenchez-Moreno M Orecchioni R Manetti F Sgarrella Y AsaraJM Peula-Garciacutea JA Marchal R Madeddu LG Delogu Immune cell impactof three differently coated lipid nanocapsules pluronic chitosan andpolyethylene glycol Sci Rep 6 (2016) 18423 httpdxdoiorg101038srep18423
[38] C Sturesson J Carlfors Incorporation of protein in PLG-microspheres withretention of bioactivity J Controlled Release 67 (2000) 171ndash178 httpdxdoiorg101016S0168-3659(00)00205-4
[39] L Ying S Jiali J Guoqiang Z Jia D Fuxin In vitro evaluation oflysozyme-loaded microspheres in thermosen- sitive methylcellulose-basedhydrogel Chin J Chem Eng 15 (2007) 566ndash572
[40] C Cai U Bakowsky E Rytting AK Schaper T Kissel Charged nanoparticlesas protein delivery systems a feasibility study using lysozyme as modelprotein Eur J Pharm Biopharm 69 (2008) 31ndash42 httpdxdoiorg101016jejpb200710005
[41] A Paillard-Giteau VT Tran O Thomas X Garric J Coudane S Marchal IChourpa JP Benoicirct CN Montero-Menei MC Venier-Julienne Effect ofvarious additives and polymers on lysozyme release from PLGA microspheresprepared by an sow emulsion technique Eur J Pharm Biopharm 75 (2010)128ndash136 httpdxdoiorg101016jejpb201003005
[42] MJ Santander-Ortega D Bastos-Gonzaacutelez JL Ortega-Vinuesa MJ AlonsoInsulin-loaded PLGA nanoparticles for oral administration an in vitrophysico-chemical characterization J Biomed Nanotechnol 5 (2009) 45ndash53httpdxdoiorg101166jbn2009022
[43] ID Rosca F Watari M Uo Microparticle formation and its mechanism insingle and double emulsion solvent evaporation J Controlled Release 99(2004) 271ndash280 httpdxdoiorg101016jjconrel200407007
[44] S Pezennec F Gauthier C Alonso F Graner T Croguennec G Bruleacute ARenault The protein net electric charge determines the surface rheologicalproperties of ovalbumin adsorbed at the air-water interface FoodHydrocolloids 14 (2000) 463ndash472 httpdxdoiorg101016S0268-005X(00)00026-6
[45] A Torcello-Goacutemez MJ Santander-Ortega JM Peula-Garciacutea JMaldonado-Valderrama MJ Gaacutelvez-Ruiz JL Ortega-Vinuesa AMartiacuten-Rodriacuteguez Adsorption of antibody onto pluronic F68-coverednanoparticles link with surface properties Soft Matter 7 (2011) 8450 httpdxdoiorg101039c1sm05570d
[46] MJ Santander-Ortega MV Lozano-Loacutepez D Bastos-Gonzaacutelez JMPeula-Garciacutea JL Ortega-Vinuesa Novel core-shell lipid-chitosan andlipid-poloxamer nanocapsules stability by hydration forces Colloid PolymSci 288 (2010) 159ndash172 httpdxdoiorg101007s00396-009-2132-y
[47] A Torcello-Goacutemez MJ Santander-Ortega JM Peula-Garciacutea JMaldonado-Valderrama MJ Gaacutelvez-Ruiz JL Ortega-Vinuesa AMartiacuten-Rodriacuteguez Adsorption of antibody onto Pluronic F68-coverednanoparticles link with surface properties Soft Matter 7 (2011) httpdxdoiorg101039c1sm05570d
[48] JP Penaloza V Maacuterquez-Miranda M Cabana-Brunod R Reyes-Ramiacuterez FMLlancalahuen C Vilos F Maldonado-Biermann LA Velaacutesquez JA FuentesFD Gonzaacutelez-Nilo M Rodriacuteguez-Diacuteaz C Otero Intracellular trafficking andcellular uptake mechanism of PHBV nanoparticles for targeted delivery inepithelial cell lines J Nanobiotechnol 15 (2017) 1 httpdxdoiorg101186s12951-016-0241-6
[49] SP Schwendeman RB Shah BA Bailey AS Schwendeman Injectablecontrolled release depots for large molecules J Controlled Release 190 (2014)240ndash253 httpdxdoiorg101016jjconrel201405057
[50] H Wang SCG Leeuwenburgh Y Li JA Jansen The use of micro- andnanospheres as functional components for bone tissue regeneration TissueEng Part B Rev 18 (2012) 24ndash39 httpdxdoiorg101089tenteb20110184
[51] JK Vasir V Labhasetwar Biodegradable nanoparticles for cytosolic deliveryof therapeutics Adv Drug Deliv Rev 59 (2007) 718ndash728 httpdxdoiorg101016jaddr200706003
[52] B Yameen W Il Choi C Vilos A Swami J Shi OC Farokhzad Insight intonanoparticle cellular uptake and intracellular targeting J Controlled Release190 (2014) 485ndash499 httpdxdoiorg101016jjconrel201406038
[53] H Sneh-Edri D Likhtenshtein D Stepensky Intracellular targeting of PLGAnanoparticles encapsulating antigenic peptide to the endoplasmic reticulumof dendritic cells and its effect on antigen cross-presentation in vitro MolPharm 8 (2011) 1266ndash1275 httpdxdoiorg101021mp200198c
[54] MJ Santander-Ortega AB Joacutedar-Reyes N Csaba D Bastos-Gonzaacutelez JLOrtega-Vinuesa Colloidal stability of Pluronic F68-coated PLGAnanoparticles a variety of stabilisation mechanisms J Colloid Interface Sci302 (2006) 522ndash529 httpdxdoiorg101016jjcis200607031
[55] B Baumann T Jungst S Stichler S Feineis O Wiltschka M Kuhlmann MLindn J Groll Control of nanoparticle release kinetics from 3D printedhydrogel scaffolds Angew Chem ndash Int Ed 56 (2017) 4623ndash4628 httpdxdoiorg101002anie201700153
[56] S-J Lee W Zhu L Heyburn M Nowicki B Harris LG Zhang Developmentof novel 3-D printed scaffolds with core-shell nanoparticles for nerveregeneration IEEE Trans Biomed Eng 64 (2017) 408ndash418 httpdxdoiorg101109tbme20162558493
[57] DJ Hines DL Kaplan Poly(lactic-co-glycolic) acid-controlled-releasesystems experimental and modeling insights Crit Rev Ther Drug CarrierSyst 30 (2013) 257ndash276 httpdxdoiorg101615CritRevTherDrugCarrierSyst2013006475
[58] H Begam SK Nandi B Kundu A Chanda Strategies for delivering bonemorphogenetic protein for bone healing Mater Sci Eng C 70 (2016)856ndash869 httpdxdoiorg101016jmsec201609074
[59] YH Kim Y Tabata Dual-controlled release system of drugs for boneregeneration Adv Drug Deliv Rev 94 (2015) 28ndash40 httpdxdoiorg101016jaddr201506003
[60] N Rescignano L Tarpani A Romani I Bicchi S Mattioli C Emiliani L TorreJM Kenny S Martino L Latterini I Armentano In-vitro degradation of PLGAnanoparticles in aqueous medium and in stem cell cultures by monitoring thecargo fluorescence spectrum Polym Degrad Stab 134 (2016) 296ndash304httpdxdoiorg101016jpolymdegradstab201610017
[61] M Morille T Van-Thanh X Garric J Cayon J Coudane D Noeumll MCVenier-Julienne CN Montero-Menei New PLGA-P188-PLGA matrix enhancesTGF-3 release from pharmacologically active microcarriers and promoteschondrogenesis of mesenchymal stem cells J Control Release 170 (2013)99ndash110 httpdxdoiorg101016jjconrel201304017
[62] A Bohr F Wan J Kristensen M Dyas E Stride S Baldursdottiacuter MEdirisinghe M Yang Pharmaceutical microparticle engineering withelectrospraying the role of mixed solvent systems in particle formation andcharacteristics J Mater Sci Mater Med 26 (2015) 61 httpdxdoiorg101007s10856-015-5379-5
[63] A Rafati A Boussahel KM Shakesheff AG Shard CJ Roberts X Chen DJScurr S Rigby-Singleton P Whiteside MR Alexander MC Davies Chemicaland spatial analysis of protein loaded PLGA microspheres for drug deliveryapplications J Control Release 162 (2012) 321ndash329 httpdxdoiorg101016jjconrel201205008
[64] R Gaudana M Gokulgandhi V Khurana D Kwatra AK Mitra Design andevaluation of a novel nanoparticulate-based formulation encapsulating a HIPcomplex of lysozyme Pharm Dev Technol 18 (2013) 752ndash759 httpdxdoiorg103109108374502012737806
[65] S Xiong X Zhao BC Heng KW Ng JSC Loo Cellular uptake of Poly-(DL-lactide-co-glycolide) (PLGA) nanoparticles synthesized through solventemulsion evaporation and nanoprecipitation method Biotechnol J 6 (2011)501ndash508 httpdxdoiorg101002biot201000351
[66] J Panyam V Labhasetwar Dynamics of endocytosis and exocytosis of poly (DL-lactide-co-glycolide) nanoparticles in vascular smooth muscle cells PharmRes 20 (2003) 212ndash220 httpwwwncbinlmnihgovpubmed12636159
pharmaceutics
Article
Formulation Colloidal Characterization and In VitroBiological Ecrarrect of BMP-2 Loaded PLGANanoparticles for Bone Regeneration
Teresa del Castillo-Santaella 1 Inmaculada Ortega-Oller 2 Miguel Padial-Molina 2 Francisco OrsquoValle 3 Pablo Galindo-Moreno 2 Ana Beleacuten Joacutedar-Reyes 14 andJoseacute Manuel Peula-Garciacutea 15
1 Biocolloid and Fluid Physics Group Department of Applied Physics University of Granada 18071 GranadaSpain
2 Department of Oral Surgery and Implant Dentistry University of Granada 18071 Granada Spain3 Department of Pathology School of Medicine amp IBIMER University of Granada 18071 Granada Spain4 Excellence Research Unit ldquoModeling Naturerdquo (MNat) University of Granada 18071 Granada Spain5 Department of Applied Physics II University of Malaga 29071 Malaga Spain Correspondence jmpeulaumaes Tel +34-952132722
Received 20 June 2019 Accepted 31 July 2019 Published 3 August 2019$amp($amp
Abstract Nanoparticles (NPs) based on the polymer poly (lactide-co-glycolide) acid (PLGA) havebeen widely studied in developing delivery systems for drugs and therapeutic biomolecules due tothe biocompatible and biodegradable properties of the PLGA In this work a synthesis method forbone morphogenetic protein (BMP-2)-loaded PLGA NPs was developed and optimized in order tocarry out and control the release of BMP-2 based on the double-emulsion (wateroilwater WOW)solvent evaporation technique The polymeric surfactant Pluronic F68 was used in the synthesisprocedure as it is known to have an ecrarrect on the reduction of the size of the NPs the enhancement oftheir stability and the protection of the encapsulated biomolecule Spherical solid polymeric NPswere synthesized showing a reproducible multimodal size distribution with diameters between100 and 500 nm This size range appears to allow the protein to act on the cell surface and at thecytoplasm level The ecrarrect of carrying BMP-2 co-adsorbed with bovine serum albumin on the NPsurface was analyzed The colloidal properties of these systems (morphology by SEM hydrodynamicsize electrophoretic mobility temporal stability protein encapsulation and short-term release profile)were studied The ecrarrect of both BMP2-loaded NPs on the proliferation migration and osteogenicdicrarrerentiation of mesenchymal stromal cells from human alveolar bone (ABSC) was also analyzedin vitro
Keywords BMP-2 PLGA nanoparticles Pluronic F68
1 Introduction
In the context of nanomedicine tissue regeneration using colloidal micro- and nano-structureshaving unique size and surface activity has received increasing attention over recent years Many ecrarrortshave been made to improve the engineering of these nano-systems in order to reach a ldquosmartrdquo deliveryof bioactive molecules in order to optimize their therapeutic advantages and minimize harmful sideecrarrects [1] With this aim a broad spectrum of biocompatible nanocarriers has been described showingproperties suitable for dicrarrerent biological and therapeutic applications [2] Among these variedproposals polymeric nanosystems represent a major group in which poly lactic-co-glycolic acid (PLGA)is one of the most widely used due to its biocompatibility biodegradability and low cytotoxicitygaining the approval from dicrarrerent drug agencies for human use [34]
Pharmaceutics 2019 11 388 doi103390pharmaceutics11080388 wwwmdpicomjournalpharmaceutics
Pharmaceutics 2019 11 388 2 of 18
PLGA-based structures are described as micro- and nanocarriers to deliver a wide variety of activemolecules and drugs synthetic or natural molecules with hydrophilic or hydrophobic properties andbiomolecules from proteins to nucleic acids [5ndash7] PLGA micro- and nanosystems can be set up usingdicrarrerent formulation techniques with the possibility of a systemic or local distribution These systemscan be applied not only in tissue regeneration but also in very diverse therapies Anticancer drugdelivery infections inflammatory diseases or gene therapy [3] Despite this great potential certainapplications especially in protein encapsulation are hindered by problems such as an uncontrolledrelease profile and protein denaturation [8ndash11]
The water-in oil-in water (WOW) double emulsion method is an ldquoemulsion solvent evaporationrdquotechnique frequently used to encapsulate hydrophilic molecules as proteins in PLGA NPs [612]The appropriate choice of organic solvents the use of polymer-surfactant blends and the addition ofstabilizer-protective agents have proved to be key aspects for optimizing the resulting systems [911]Additionally a surface specific functionalization can be used to improve their versatility allowingthe chemical surface immobilization of dicrarrerent molecules in order to confer targeting or adhesiveproperties to these nanocarriers [13]
Within tissue engineering bone regeneration has a broad range of applications mostly in the fieldof dentistry where PLGA is suggested as a reference polymer to formulate NPs with bone-healinguses [14] The literature describes the delivery of bioactive molecules normally growth factors usingpolymeric microparticles (MPs) and NPs with PLGA as the main component [13] Among the bonemorphogenetic growth factors BMP-2 (bone morphogenetic protein 2) has been the most frequentlycited with many examples in which encapsulation or surface adsorption enables adequate entrapmenteciency and diverse release patterns [15ndash19] For proteins with a very short half-life such as BMPsbiodegradable PLGA nanosystems provide protection and optimal dosage for an adequate stimulationof cell dicrarrerentiation [2021]
Thus within this scenario in the present work we seek to optimize a nano-particulate system inorder to carry out and control the release of BMP-2 using as a starting point the synthesis procedure ofa lysozyme-loaded NP system previously described for the encapsulation of that model protein [11]Also to encapsulate BMP-2 we prepared a second system in which this protein was co-adsorbedwith bovine serum albumin onto the surface of empty NPs The size and morphology the proteinencapsulation eciency the surface characteristics and the colloidal and temporal stability werestudied to complete the physico-chemical characterization of both NP systems
The release profile of BMP-2 indicates the potential of a PLGA nanocarrier for bone regenerationand depends heavily on the polymer degradation by hydrolysis [22] However over the shortterm during which the release does not depend on this chemical degradation proper control ofrelease is necessary in order to modulate other physical processes Thus we focused our releaseexperiments on the short-term using dicrarrerent techniques to compare the two NP samples and establishthe corresponding BMP-2 release profiles Finally the biological activity (cell migration proliferationand osteogenic dicrarrerentiation) was tested in vitro using mesenchymal stromal cells (MSCs) derivedfrom alveolar bone [23]
2 Materials and Methods
21 Nanoparticle Synthesis
211 Formulation
Poly(lactide-co-glycolide) acid (PLGA 5050) ([C2H2O2]x[C3H4O2]y) x = 50 y = 50 (Resomerreg
503H (Evonik Essen Germany) 32ndash44 kDa was used as the polymer and polymeric surfactantPluronic F68 (Poloxamer 188) (Sigma-Aldrich St Louis MO USA) as the emulsifier Their structurebased on a poly(ethylene oxide)-block-poly(propylene oxide)-block-poly(ethylene oxide) is expressedas PEOa-PPOb-PEOa with a = 75 and b = 30 Human recombinant bone morphogenetic proteinrhBMP-2 (Sigma-H4791) was used as therapeutic biomolecule Water was purified in a Milli-Q
Pharmaceutics 2019 11 388 3 of 18
Academic Millipore system A double-emulsion synthesis method was used following a procedurepreviously described with slight modifications [11] In this method 100 mg of PLGA and 3 mg ofdeoxycholic acid (DC) were dissolved in a tube containing 1 mL of ethyl acetate (EA) and vortexedIn total 40 microL of a bucrarrered solution at pH 128 with or without rhBMP-2 (200 microgmL) were addedand immediately sonicated (Branson Ultrasonics 450 Analog Sonifier) for 1 min (Duty cycle dial 20Output control dial 4) with the tube surrounded by ice This primary WO emulsion was poured intoa plastic tube containing 2 mL of a bucrarrered solution (pH 12) of F68 at 1 mgmL and vortexing for30 s Then the tube surrounded by ice was sonicated at the maximum amplitude for the micro tip for1 min (Output control 7) This second WOW emulsion was poured into a glass containing 10 mL ofthe bucrarrered F68 solution and kept under magnetic stirring for 2 min The organic solvent was thenrapidly extracted by evaporation under vacuum to a final volume of 8 mL The resulting empty andBMP-2 encapsulated NP systems were named NP and NP-BMP2 respectively A detailed scheme ofthe synthesis procedure with a yield based on the PLGA component always higher than 85 is shownin Figure S1 of the Supplementary Materials
212 Cleaning and Storage
After the organic solvent evaporation the sample was centrifuged for 10 min at 20 C at 12000 rpmThe supernatant was filtered using Millipore nanofilters 01microm for measuring the free non-encapsulatedprotein The pellet was then resuspended in phosphate bucrarrer (115 mM NaH2PO4) PB to a finalvolume of 4 mL and kept refrigerated at 4 C Under these conditions the systems kept colloidalstability at least for one month
213 Protein Loading and Encapsulation Eciency
The initial protein loading was optimized for the nanoparticle formulation preserving the finalcolloidal stability after the evaporation step and taking into account the amounts shown in the literaturefor this growth factor when encapsulated inside PLGA NPs [2425] Thus we chose 2 microg as the initialtotal mass of rhBMP-2 which means a relation of 2 105 ww (rhBMP-2PLGA) The amount ofencapsulated rhBMP-2 was calculated by measuring the dicrarrerence between the initial added amountand the free non-encapsulated protein present in the supernatant after the cleaning step which wastested by a specific enzyme-linked immuno-sorbent assay following the instructions of the manufacturer(ELISA kit RAB0028 from Sigma-Aldrich St Louis MO USA) Then protein-encapsulation eciency(EE) was calculated as follows
EE =MI MF
MI 100
where MI is the initial total mass of rhBMP-2 and MF is the total mass of rhBMP-2 in theaqueous supernatant
214 Physical Protein Adsorption
Bovine serum albumin (BSA) and rhBMP-2 were coupled on the empty nanoparticle surface bya physical adsorption method The appropriate volume of an aqueous protein solution containing05 mg of BSA and 2 microg of rhBMP-2 was mixed with 5 mL of acetate bucrarrer (pH 5) containing emptyNPs with 125 mg of PLGA This provided a starting amount of proteins corresponding to 004 ww(proteinPLGA) while the mass relation between proteins was 04 ww (rhBMP-2BSA) This solutionwas incubated at room temperature for 2 h under mechanical stirring The nanoparticles were separatedfrom the bucrarrer solution by centrifugation and after the supernatants were filtered (Millipore nanofilters01 microm) they were qualitatively analyzed by gel electrophoresis while the protein quantification wasmade by a bicinchoninic acid protein assay (BCA) (Sigma-Aldrich St Louis MO USA) for BSA andthe specific ELISA for rhBMP-2 The nanoparticle pellet was resuspended in phosphate bucrarrer (pH 74)and stored at 4 C This system was named NP-BSA-BMP2
Pharmaceutics 2019 11 388 4 of 18
215 Protein Separation by Gel Electrophoresis SDS-PAGE
The protein-loaded NPs and dicrarrerent supernatants were treated at 90 C for 10 min in thefollowing bucrarrer 625 mM Tris-HCl (pH 68 at 25 C) 2 (wv) sodium dodecyl sulfate (SDS) 10glycerol 001 (wv) bromophenol blue 40 mM dithiothreitol (DTT) Samples were then separated bysize in porous 12 polyacrylamide gel (1D SDS polyacrylamide gel electrophoresis) under the ecrarrectof an electric field The electrophoresis was run under constant voltage (130 V 45 min) and the gelswere stained using a Coomassie Blue solution (01 Coomassie Brilliant Blue R-250 50 methanol and10 glacial acetic acid) and destained with the same solution lacking the dye
22 Nanoparticle Characterization Morphology Size Concentration and Electrokinetic Mobility
NPs were imaged by scanning electron microscopy (SEM) with a Zeiss SUPRA 40VP field-emissionscanning electron microscope from the Scientific Instrumentation Center of the University of Granada(CIC UGR)
The hydrodynamic size distribution of the NPs was evaluated by nanoparticle tracking analysis(NTA) with a NanoSight LM10-HS (GB) FT14 (NanoSight Amesbury UK) and an sCMOS cameraThe particle concentration according to the diameter (size distribution) was calculated as an averageof at least three independent size distributions The total concentration of NPs of each system wasdetermined in order to control the number of particles used in cell experiments The measurementconditions for all samples were 25 C a viscosity of 089 cP a measurement time of 60 s and a cameragain of 250 The camera shutter was 11 and 15 ms for the empty and BMP-loaded NPs respectivelyThe detection threshold was fixed at 5
The electrophoretic mobility of the NPs was determined using a Zetasizerreg NanoZeta ZS device(Malvern Instrument Ltd Malvern UK) working at 25 C with an He-Ne laser of 633 nm and a 173
scattering angle Each data point was taken as an average over three independent sample measurementsFor each sample the electrophoretic mobility distribution and the average electrophoretic mobility(micro-average) were determined by the technique of laser Doppler electrophoresis
23 Colloidal and Temporal Stability in Biological Media
The average hydrodynamic diameter and the polydispersity index (PDI) by dynamic lightscattering (DLS) of each NP system were measured in dicrarrerent media (phosphate bucrarrer (PB) salinephosphate bucrarrer (PBS) and cell culture medium Dulbeccorsquos modified Eaglersquos medium DMEM(Sigma)) Also data on temporal stability were gathered by repeating these analyses at dicrarrerent timesafter synthesis (0 1 and 5 days) and after 1 month under storage conditions
In vitro release experiments were conducted as follows 1 mL of each sample for each incubationtime was suspended in PBS at 37 C After the corresponding time (24 48 96 168 h) NPs wereseparated from the supernatant of released proteins by centrifugation for 10 min at 14000 rpm (10 C)The NP pellet was suspended in 1 mL of 005 M NaOH and stirred for 2 h for a complete polymerdegradation The alkaline protein solution was assayed by BCA and ELISA to quantify the unreleasedamount The protein released was calculated taking into account the total encapsulated amount Allexperiments were made in triplicate
24 Cell Interactions
For all biological in vitro studies a cell population cultured from the maxillary alveolar bonewas used This population was previously characterized and confirmed to present all characteristicsof a mesenchymal stromal cell population (MSC) [23] Cells were taken from healthy humandonors after the approval from the Ethics Committee for Human Research from the University ofGranada (424CEIH2018) Regular Dulbeccorsquos modified Eaglersquos medium (DMEM) with 1 gL glucose(DMEM-LG) (Gibco) 10 fetal bovine serum (FBS) (Sigma-Aldrich St Louis MO USA) 1100 ofnon-essential amino acid solution (NEAA) (Gibco) 001 microgmL of basic fibroblast growth factor (bFGF)
Pharmaceutics 2019 11 388 5 of 18
(PeproTech London UK) 100 UmL of penicillinstreptomycin and 025 microgmL of amphotericin Bwas used as culture medium for all experiments Cultures were maintained at 37 C in a 5 CO2atmosphere (2000 cellswell) All biological experiments were repeated in triplicate at least 3 timesper condition
241 Cell Migration
A cell-migration assay was conducted as previously described [2627] Briefly MSCs weredistributed on to three wells for each condition and allowed to grow to a cell confluency close to99 in 24-wellsplate at 3000 cellscm2 and in each well three dicrarrerent scratches were made Thencells were starved for 24 h by adding culture medium without serum A scratch was made usinga pipette tip along the diameter of the well A wash step with PBS was performed to remove thescratched cells Fresh complete culture media was added and supplemented depending on the assignedgroup (BMP-2 NP- BMP2 and NP-BSA-BMP2 at 125 25 and 5 ngmL of BMP-2) Afterwardsnine images were taken from the same area in each condition until 48 h later On these images thescraped area was measured by ImageJ software (National Institute of Health Bethesda MD USAhttprsbwebnihgovij) The reduction in the scratched area over time was measured consideringthe area at time 0 as 100 open
242 Cell Proliferation
Proliferation was evaluated by a sulphorhodamine (SRB) assay [28] The assay was conducted byseeding the cells at 1500 cellscm2 in a 96-well plate at a confluence not higher than 50 After cellattachment the dicrarrerent supplements were added (BMP-2 NP- BMP2 and NP-BSA-BMP2 at 12525 and 5 ngmL of BMP-2) and the cells were maintained in culture for up to 7 days At each timepoint the cells were washed with 1X PBS and fixed by adding ice-cold 10 trichloroacetic acid for20 min at 4 C Then the cells were washed 3 times with dH2O and dried until all time points werecollected Each well received 04 SRB in 1 acetic acid for 20 min at room temperature with gentleshaking The staining was finished by washing each well 3 times with 1 acetic acid and drying it atroom temperature for 24 h The dye was retrieved from the cells by adding 10 mM Tris Base at pH 105and gently shaking for 10 min The solution recovered was then distributed in a 96-well plate and theoptical absorbance was read at 492 nm
243 Osteogenic Dicrarrerentiation
Osteogenic dicrarrerentiation was evaluated by adding osteogenic media to the cell culture incombination with free BMP-2 NP-BMP2 and NP-BSA-BMP2 at the highest dosages used inprevious experiments Cells were seeded at 3000 cellscm2 and cultured to reach an 85 to90 confluency This was followed by the addition of induction media containing 10 mM of-glycerophosphate (Fluka 50020) 01 microM of dexamethasone (Sigma-Aldrich D2915) and 005 mMof L-ascorbic acid (Sigma-Aldrich A8960) Cell cultures were maintained for 7 days to analyzeearly activity At day 7 cells were collected in 1 mL of TRIzolreg Then RNA was extracted andconverted to cDNA Alkaline phosphatase (ALP) was then evaluated expression being calculatedrelative to glyceraldehyde-3-phosphate dehydrogenase protein (GAPDH) by the 2DDCt methodThese procedures were conducted as described elsewhere [23] Forward and reverse primer sequenceswere AGCTCATTTCCTGGTATGACAAC and TTACTCCTTGGAGGCCATGTG for GAPDH andTCCAGGGATAAAGCAGGTCTTG and CTTTCTCTTTCTCTGGCACTAAGG for ALP
244 Statistical Evaluation
Cell migration and proliferation were evaluated by ANOVA followed by Tukey multiplecomparisons test for pairwise analysis Comparison between the levels of ALP at 4 vs 7 dayswere analyzed by paired Studentrsquos t test In all cases a p value lower than 005 was established asstatistical significance
Pharmaceutics 2019 11 388 6 of 18
3 Results and Discussion
31 Nanoparticle Formulation
Double emulsion-solvent evaporation has been described as a robust and frequently used methodto produce biomolecule-loaded PLGA NPs [6121329] A formulation previously optimized byour group enabled the preservation of the biological activity of encapsulated biomolecules using aslightly aggressive organic solvent Moreover deoxycholic acid has been used in the first step of theformulation in order to improve the colloidal stability of NPs and simultaneously to obtain NP surfacesenriched with carboxylic groups improving their versatility and allowing a subsequent chemicalimmobilization of dicrarrerent specific ligands [30] By means of this improved formulation in the presentwork we developed empty nanoparticles (NPs) or nanoparticles encapsulating rhBMP-2 (NP-BMP2)A schematic description of the synthesis procedure is shown in Figure S1 of the Supplementary DataFor NP-BMP2 we achieved a protein-encapsulation eciency (EE) of 97 plusmn 2 This result is consistentwith the literature in which several authors have reported similarly high values encapsulating thisprotein inside PLGA nano- and microparticles [3132] Our formulation has several factors leading tothis very high EE value The low proteinpolymer relation in mass [33] the anity of rhBMP-2 to anunspecific interaction with hydrophobic surfaces [31] or the addition of stabilizers (poloxamer) inthe second step of the double-emulsion procedure [13] The absence of rhBMP-2 in the supernatantresulting from the centrifugation step in the cleaning process was verified by ELISA and SDS-PAGE inwhich a clear band corresponding to 14 kD of rhBMP-2 polypeptidic chains is shown for lane A inFigure 1 corresponding to NP-BMP2 The mass of protein encapsulated around 2 microg is similar to thatof dicrarrerent PLGA micro- and nanosystems described in the literature [183435] Taking into accountthe storage conditions for our samples this corresponds to 500 ngmL which represents a sucientconcentration for practical applications since this growth factor shows in vitro biological activities atvery low dosages (5ndash20 ngmL) [13]
Pharmaceutics 2019 11 x 6 of 18
31 Nanoparticle Formulation
Double emulsion-solvent evaporation has been described as a robust and frequently used method to produce biomolecule-loaded PLGA NPs [6121329] A formulation previously optimized by our group enabled the preservation of the biological activity of encapsulated biomolecules using a slightly aggressive organic solvent Moreover deoxycholic acid has been used in the first step of the formulation in order to improve the colloidal stability of NPs and simultaneously to obtain NP surfaces enriched with carboxylic groups improving their versatility and allowing a subsequent chemical immobilization of different specific ligands [30] By means of this improved formulation in the present work we developed empty nanoparticles (NPs) or nanoparticles encapsulating rhBMP-2 (NP-BMP2) A schematic description of the synthesis procedure is shown in Figure S1 of the Supplementary Data For NP-BMP2 we achieved a protein-encapsulation efficiency (EE) of 97 plusmn 2 This result is consistent with the literature in which several authors have reported similarly high values encapsulating this protein inside PLGA nano- and microparticles [3132] Our formulation has several factors leading to this very high EE value The low proteinpolymer relation in mass [33] the affinity of rhBMP-2 to an unspecific interaction with hydrophobic surfaces [31] or the addition of stabilizers (poloxamer) in the second step of the double-emulsion procedure [13] The absence of rhBMP-2 in the supernatant resulting from the centrifugation step in the cleaning process was verified by ELISA and SDS-PAGE in which a clear band corresponding to 14 kD of rhBMP-2 polypeptidic chains is shown for lane A in Figure 1 corresponding to NP-BMP2 The mass of protein encapsulated around 2 microg is similar to that of different PLGA micro- and nanosystems described in the literature [183435] Taking into account the storage conditions for our samples this corresponds to 500 ngmL which represents a sufficient concentration for practical applications since this growth factor shows in vitro biological activities at very low dosages (5ndash20 ngmL) [13]
Figure 1 SDS polyacrylamide gel electrophoresis (SDS-PAGE) analysis under reducing conditions of solid PLGA Nanoparticles (PLGA NPs) and liquid (supernatant) fractions of different NP systems Lane P Protein standards lane A NP-BMP2 (bone morphogenetic protein) lane B supernatant of NP-BMP2 after synthesis and encapsulation of rhBMP-2 lane C NP after physical adsorption of BSArhBMP-2 lane D supernatant after physical adsorption of BSA(bovine serum albumin)rhBMP-2 on NP system
On the other hand a second nanosystem resulted modifying the way in which rhBMP-2 is incorporated in the nanocarrier There are several examples of surface adsorption of different growth factors in micro- and nanoparticles [35ndash37] and surface immobilization over the encapsulation has recently been proposed as a way to modulate the later release of biomolecules This process which
Figure 1 SDS polyacrylamide gel electrophoresis (SDS-PAGE) analysis under reducing conditions ofsolid PLGA Nanoparticles (PLGA NPs) and liquid (supernatant) fractions of dicrarrerent NP systems LaneP Protein standards lane A NP-BMP2 (bone morphogenetic protein) lane B supernatant of NP-BMP2after synthesis and encapsulation of rhBMP-2 lane C NP after physical adsorption of BSArhBMP-2lane D supernatant after physical adsorption of BSA(bovine serum albumin)rhBMP-2 on NP system
On the other hand a second nanosystem resulted modifying the way in which rhBMP-2 isincorporated in the nanocarrier There are several examples of surface adsorption of dicrarrerent growthfactors in micro- and nanoparticles [35ndash37] and surface immobilization over the encapsulation has
Pharmaceutics 2019 11 388 7 of 18
recently been proposed as a way to modulate the later release of biomolecules This process whichdepends on the slow dicrarrusion of biomolecules through the polymeric matrix is consequently highlyinfluenced by the proteinndashpolymer interaction [3839] and polymer degradation [36] Thus this newfocus on the use of PLGA NPs for biomolecule delivery was explored by immobilizing the proteinrhBMP-2 on the surface of empty NPs by means of simple physical adsorption This process is knownto be governed by electrostatic and hydrophobic interactions between protein molecules and NPsurfaces [40]
For this the surface-charged groups the hydrophilicity the net charge of the protein moleculesand the characteristics of the adsorption medium are the reference parameters Thus we designed aco-adsorption experiment in which a mixture of rhBMP-2 and BSA (04 ww rhBMP-2BSA) interactsimultaneously with the PLGA NP surface Albumins are routinely used as protective proteins whengrowth factors are incorporated in PLGA NPs [1319] Moreover a surface distribution of BSA moleculescan improve the colloidal stability of NPs at physiological pH due to their net negative charge underthese conditions [41] Figure S2 from Supplementary Materials shows a scheme of the co-adsorptionprocess The adsorption eciency is higher than 95 and in SDS-PAGE from Figure 1 two bandscharacteristic of both proteins can be seen in lane C corresponding to the NP-BSA-BMP2 nanosystemHowever lane D corresponding to the run of the supernatant from the centrifugation of the nanosystemafter adsorption processes shows the absence of any protein This result is fully explained by takinginto account the pH of the medium (pH 50) near the isoelectric point of BSA where the adsorption ofthis protein onto negatively charged nanoparticles presents a maximum [4042] The immobilizationof rhBMP-2 on the negatively charged surface of NPs proves they are electrostatically favored due tothe positive net charge of this protein at acid and neutral pH
32 Nanoparticle Characterization
321 Nanoparticle Size
SEM and STEM micrographs (Figure 2) show that the samples consist of spherical particles ofdicrarrerent diameters (between 150 and 450 nm) a range similar to that found in a previous work inwhich NPs were loaded with lysozyme following a similar synthesis protocol [11] In that workthe DLS technique failed to provide a reliable size distribution Therefore the NTA technique wasdirectly used to determine the hydrodynamic size of the BMP2-loaded NPs (see NTA video in theSupplementary Material)
The size distributions for empty (NP) and BMP-loaded NPs (NP-BMP2) from NTA (Figure 3 andvideos S1 S2) were consistent with the SEM images Particles with diameters between 100 and 500 nmwere found to have the highest particle concentration at around 200 nm The loading with BMP hadan ecrarrect on the size distribution leading to more defined peaks These measurements enabled usto determine the concentration of particles in the measured sample 688 plusmn 009 108 ppmL and519 plusmn 012 108 ppmL for NP and NP-BMP2 nanosystems respectively These values were used(by taking into account the corresponding dilution) to control the number of particles added in thecell experiments
Pharmaceutics 2019 11 388 8 of 18
Pharmaceutics 2019 11 x 7 of 18
depends on the slow diffusion of biomolecules through the polymeric matrix is consequently highly influenced by the proteinndashpolymer interaction [3839] and polymer degradation [36] Thus this new focus on the use of PLGA NPs for biomolecule delivery was explored by immobilizing the protein rhBMP-2 on the surface of empty NPs by means of simple physical adsorption This process is known to be governed by electrostatic and hydrophobic interactions between protein molecules and NP surfaces [40]
For this the surface-charged groups the hydrophilicity the net charge of the protein molecules and the characteristics of the adsorption medium are the reference parameters Thus we designed a co-adsorption experiment in which a mixture of rhBMP-2 and BSA (04 ww rhBMP-2BSA) interact simultaneously with the PLGA NP surface Albumins are routinely used as protective proteins when growth factors are incorporated in PLGA NPs [1319] Moreover a surface distribution of BSA molecules can improve the colloidal stability of NPs at physiological pH due to their net negative charge under these conditions [41] Figure S2 from Supplementary Materials shows a scheme of the co-adsorption process The adsorption efficiency is higher than 95 and in SDS-PAGE from Figure 1 two bands characteristic of both proteins can be seen in lane C corresponding to the NP-BSA-BMP2 nanosystem However lane D corresponding to the run of the supernatant from the centrifugation of the nanosystem after adsorption processes shows the absence of any protein This result is fully explained by taking into account the pH of the medium (pH 50) near the isoelectric point of BSA where the adsorption of this protein onto negatively charged nanoparticles presents a maximum [4042] The immobilization of rhBMP-2 on the negatively charged surface of NPs proves they are electrostatically favored due to the positive net charge of this protein at acid and neutral pH
32 Nanoparticle Characterization
321 Nanoparticle Size
SEM and STEM micrographs (Figure 2) show that the samples consist of spherical particles of different diameters (between 150 and 450 nm) a range similar to that found in a previous work in which NPs were loaded with lysozyme following a similar synthesis protocol [11] In that work the DLS technique failed to provide a reliable size distribution Therefore the NTA technique was directly used to determine the hydrodynamic size of the BMP2-loaded NPs (see NTA video in the Supplementary Material)
Figure 2 Scanning electron microscopy (SEM) micrograph of rhBMP-2-loaded nanoparticles (NP-BMP2)
Figure 2 Scanning electron microscopy (SEM) micrograph of rhBMP-2-loaded nanoparticles(NP-BMP2)
Pharmaceutics 2019 11 x 8 of 18
The size distributions for empty (NP) and BMP-loaded NPs (NP-BMP2) from NTA (Figure 3 and videos S1 S2) were consistent with the SEM images Particles with diameters between 100 and 500 nm were found to have the highest particle concentration at around 200 nm The loading with BMP had an effect on the size distribution leading to more defined peaks These measurements enabled us to determine the concentration of particles in the measured sample 688 plusmn 009 times 108 ppmL and 519 plusmn 012 times 108 ppmL for NP and NP-BMP2 nanosystems respectively These values were used (by taking into account the corresponding dilution) to control the number of particles added in the cell experiments
Figure 3 Hydrodynamic diameter distribution of NP (circles) and NP-BMP2 (thick black line) measured at pH 70 (phosphate buffer) by nanoparticle tracking analysis (NTA)
322 Electrokinetic Mobility and Colloidal Stability
The surface charge of nanoparticles can be analyzed using an electrokinetic study by measuring the electrophoretic mobility (microe) under different conditions Figure 4 shows the microe and zeta potential values for the three nanosystems NP NP-BMP2 and NP-BSA-BMP2 at low ionic strength and different pH values The electric surface charge of NPs resides in the carboxylic groups of the uncapped PLGA and deoxycholic acid molecules These functionalized groups are additionally useful due to the possibility of a chemical surface vectorization in order to develop directed delivery nanocarriers [43] It was previously confirmed that protonation of these acidic surface groups at pH values under their pKa value was tightly correlated with a loss of surface charge and consequently a reduction (in absolute value) of the electrophoretic mobility of the colloidal system [4445] Usually when colloidal particles are coated by protein molecules the microe values change markedly compared with the same bare surfaces and are influenced by the electrical charge of the adsorbed protein molecules [4647] The electrokinetic behavior of the NP-BMP2 system remains similar to that of NP and encapsulation of rhBMP-2 does not affect the surface charge distribution A similar result was reported by drsquoAngelo et al on encapsulating different growth factors in PLGA-poloxamer blend nanoparticles in the same proportion ww of proteinpolymer [24] This may be due to the low amount of encapsulated protein and its distribution in the inner part of the NPs (far from the surface) In our system this internal distribution may be favored by the encapsulating conditions where the basic pH (pH 120) of the water phase containing rhBMP-2 allows a negative charge of these protein molecules thereby preventing their electrostatic specific interaction with acidic groups of the NPs
0 200 400 600 80000
05
10
15
20
25
30
35
40
parti
cle
conc
entra
tion
(106 p
pm
L)
D(nm)
Figure 3 Hydrodynamic diameter distribution of NP (circles) and NP-BMP2 (thick black line) measuredat pH 70 (phosphate bucrarrer) by nanoparticle tracking analysis (NTA)
322 Electrokinetic Mobility and Colloidal Stability
The surface charge of nanoparticles can be analyzed using an electrokinetic study by measuringthe electrophoretic mobility (microe) under dicrarrerent conditions Figure 4 shows the microe and zeta potentialvalues for the three nanosystems NP NP-BMP2 and NP-BSA-BMP2 at low ionic strength anddicrarrerent pH values The electric surface charge of NPs resides in the carboxylic groups of the uncappedPLGA and deoxycholic acid molecules These functionalized groups are additionally useful due to thepossibility of a chemical surface vectorization in order to develop directed delivery nanocarriers [43]It was previously confirmed that protonation of these acidic surface groups at pH values undertheir pKa value was tightly correlated with a loss of surface charge and consequently a reduction (in
Pharmaceutics 2019 11 388 9 of 18
absolute value) of the electrophoretic mobility of the colloidal system [4445] Usually when colloidalparticles are coated by protein molecules the microe values change markedly compared with the samebare surfaces and are influenced by the electrical charge of the adsorbed protein molecules [4647]The electrokinetic behavior of the NP-BMP2 system remains similar to that of NP and encapsulationof rhBMP-2 does not acrarrect the surface charge distribution A similar result was reported by drsquoAngeloet al on encapsulating dicrarrerent growth factors in PLGA-poloxamer blend nanoparticles in the sameproportion ww of proteinpolymer [24] This may be due to the low amount of encapsulated proteinand its distribution in the inner part of the NPs (far from the surface) In our system this internaldistribution may be favored by the encapsulating conditions where the basic pH (pH 120) of the waterphase containing rhBMP-2 allows a negative charge of these protein molecules thereby preventingtheir electrostatic specific interaction with acidic groups of the NPs
Pharmaceutics 2019 11 x 9 of 18
Figure 4 Electrophoretic mobility and zeta potential vs pH in buffered media of low salinity (ionic strength equal to 0002 M) for the different nanosystems (black square) NP (blue triangle) NP-BMP2 (red circle) NP-BSA-BMP2
The electrokinetic distribution for the NP-BSA-BMP2 system radically changes As previously shown the very high adsorption efficiency leads to NPs with both proteins adsorbed around their surface This situation is closely correlated with the microe values from Figure 4 Taking into account the ww relation between adsorbed proteins (250 times higher for BSA) albumin molecules modulate the behavior at pH values below their isoelectric point (pI 47) where the positive net charge of BSA masks the original surface charge of NPs and even changes their original values to positive ones This is a typical result found for this protein-covering colloidal particles [4248] At neutral and basic pH values BSA molecules have a negative net charge and the slight decrease in the absolute microe values could be due to the reduction of the negative net surface charge of NPs which may be shielded at least in a small part by the positive charge of rhBMP-2 molecules under their basic isoelectric point (pI 90)
The colloidal stability for the different nanosystems (NP NP-BMP2 and NP-BSA-BMP2) was determined by analyzing the size distributions in various media (PB PBS and DMEM) at different times after synthesis (0 1 and 5 days) Size distributions similar to the original ones were found for the two formulations NP and NP-BMP2 in all the media analyzed This result was similar to that previously found for these types of NPs encapsulating lysozyme [11] in which the combination of electrostatic and steric interactions generated by surface chemical groups of NPs confer the stability mechanism that prevents colloidal aggregation [33] The decrease of the absolute value of the zeta potential for the NP-BSA-BMP2 system as a consequence of surface protein distribution does not affect its colloidal stability This system also maintains the same size distribution in the different media It is commonly accepted that a zeta potential higher than +30 or minus30 mV will give rise to a stable colloidal system [49] and the zeta potential value for NP-BSA-BMP2 is above minus30 mV Colloidal stability in PBS and DMEM typically used media for the development of scaffold or cell interactions
Figure 4 Electrophoretic mobility and zeta potential vs pH in bucrarrered media of low salinity (ionicstrength equal to 0002 M) for the dicrarrerent nanosystems (black square) NP (blue triangle) NP-BMP2(red circle) NP-BSA-BMP2
The electrokinetic distribution for the NP-BSA-BMP2 system radically changes As previouslyshown the very high adsorption eciency leads to NPs with both proteins adsorbed around theirsurface This situation is closely correlated with the microe values from Figure 4 Taking into account theww relation between adsorbed proteins (250 times higher for BSA) albumin molecules modulate thebehavior at pH values below their isoelectric point (pI 47) where the positive net charge of BSA masksthe original surface charge of NPs and even changes their original values to positive ones This is atypical result found for this protein-covering colloidal particles [4248] At neutral and basic pH valuesBSA molecules have a negative net charge and the slight decrease in the absolute microe values could bedue to the reduction of the negative net surface charge of NPs which may be shielded at least in asmall part by the positive charge of rhBMP-2 molecules under their basic isoelectric point (pI 90)
The colloidal stability for the dicrarrerent nanosystems (NP NP-BMP2 and NP-BSA-BMP2) wasdetermined by analyzing the size distributions in various media (PB PBS and DMEM) at dicrarrerenttimes after synthesis (0 1 and 5 days) Size distributions similar to the original ones were found for
Pharmaceutics 2019 11 388 10 of 18
the two formulations NP and NP-BMP2 in all the media analyzed This result was similar to thatpreviously found for these types of NPs encapsulating lysozyme [11] in which the combination ofelectrostatic and steric interactions generated by surface chemical groups of NPs confer the stabilitymechanism that prevents colloidal aggregation [33] The decrease of the absolute value of the zetapotential for the NP-BSA-BMP2 system as a consequence of surface protein distribution does not acrarrectits colloidal stability This system also maintains the same size distribution in the dicrarrerent media It iscommonly accepted that a zeta potential higher than +30 or 30 mV will give rise to a stable colloidalsystem [49] and the zeta potential value for NP-BSA-BMP2 is above 30 mV Colloidal stability in PBSand DMEM typically used media for the development of scacrarrold or cell interactions respectivelyassures the potential use of these nanosystems for in vitro or in vivo living environments Additionallythese systems maintained their size under storage in PB at 4 C for at least 1 month (data not shown)showing this to be an adequate medium for sample storage
323 Protein Release
One of the main problems for micro- or nanosystems of PLGA drug delivery is to find theappropriate release pattern for encapsulatedattached protein molecules A wide spectrum offormulations modulates this property by the use of dicrarrerent types of synthesis processes PLGApolymers co-polymers and stabilizers [313] An adequate limitation and control in the burst releaseis critical for BMPs in order to ensure long-term continuous release that favored by the polymerdegradation provides better in vivo action in driving bone and cartilage regeneration [20] Thereforewe previously developed a dual PLGA nanosystem for controlled short-term release where proteindicrarrusion and proteinndashpolymer interaction are the main factors governing this process [11]
In the present work NP-BMP2 and NP-BSA-BMP2 nanosystems represent two dicrarrerent ways inwhich rhBMP-2 was incorporated into the nanocarrier Figure 5A shows the cumulative release of bothproteins rhBMP-2 and BSA for dicrarrerent systems as a function of time in a short-term period (7 days)The encapsulated rhBMP-2 protein reaches an amount released of around 30 of the initial encapsulatedone while adsorbed rhBMP-2 despite its surface distribution is three times lower However BSAshows released amounts up to 80 of the initial adsorbed ones In all cases error bars correspond tothe standard deviations from three independent experiments Under these conditions the growthfactor encapsulated in NP-BMP2 presents a release pattern similar to that previously found with thesame formulation but using lysozyme as the protein [11] Poloxamer in the water phase of the synthesisprocess can be key in modulating both specific and unspecific interfacial protein interactions [50]Thus the relation between proteinndashpolymer interaction and protein dicrarrusion appears to be wellbalanced preventing an excessive initial burst and simultaneously maintaining the needed proteinflux to release around a third of the encapsulated rhBMP-2 in 7 days Although an excessive initialburst has been widely reported for PLGA NPs related with protein molecules close to the surface [6]this situation did not appear for the NP-BMP2 system this being consistent with the electrokineticbehavior that did not show the presence of protein near surface The literature ocrarrers some exampleswith reduced short-term release of BMP-2 using more hydrophilic PLGA-PEG co-polymers [16] or adicrarrerent synthesis process [25]
The release performance for the NP-BSA-BMP2 system also shown in Figure 5A presents notabledicrarrerences The electrokinetic profile has previously justified the surface location of BSA and rhBMP-2on the surface which could lead to a fast release of both proteins However results from Figure 5ABshow this trend only for the BSA protein that is released from NPs with about 20 of the initial amountremaining after seven days However up to 90 of the initial load of rhBMP-2 protein unlike BSAremains attached to the surface The NP surface with hydrophilic groups form poloxamer moleculesand a negative charge due to the abundant presence of carboxylic groups (end-groups of PLGA anddeoxycholic acid molecules) favor a desorption process for BSA whose molecules have a negativecharge under release conditions (physiological pH) This agrees with the results of other authors whoeven after encapsulating BSA in PLGA-poloxamer blend NPs achieved a fast burst release of above
Pharmaceutics 2019 11 388 11 of 18
40 to 50 of the initial protein amount [33] Moreover the co-encapsulation of albumins with growthfactors could strongly acrarrect its release profile causing an initial burst [2124] Otherwise the specificelectrostatic attraction between positive rhBMP-2 molecules and negative surface groups slows downthe short time release of this protein This result is in agreement with the low release of adsorbedBMP previously found using PLGA micro- and nanoparticles with uncapped acid end groups [3851]Thus the combination of dicrarrerent methods for trapping BMP-2 into and around NPs shows up thepossibility of attaining a properly controlled release balancing the interactions between polymersstabilizers and protein
Pharmaceutics 2019 11 x 10 of 18
respectively assures the potential use of these nanosystems for in vitro or in vivo living
environments Additionally these systems maintained their size under storage in PB at 4 degC for at
least 1 month (data not shown) showing this to be an adequate medium for sample storage
323 Protein Release
One of the main problems for micro- or nanosystems of PLGA drug delivery is to find the
appropriate release pattern for encapsulatedattached protein molecules A wide spectrum of
formulations modulates this property by the use of different types of synthesis processes PLGA
polymers co-polymers and stabilizers [313] An adequate limitation and control in the burst release
is critical for BMPs in order to ensure long-term continuous release that favored by the polymer
degradation provides better in vivo action in driving bone and cartilage regeneration [20] Therefore
we previously developed a dual PLGA nanosystem for controlled short-term release where protein
diffusion and proteinndashpolymer interaction are the main factors governing this process [11]
In the present work NP-BMP2 and NP-BSA-BMP2 nanosystems represent two different ways
in which rhBMP-2 was incorporated into the nanocarrier Figure 5A shows the cumulative release of
both proteins rhBMP-2 and BSA for different systems as a function of time in a short-term period (7
days) The encapsulated rhBMP-2 protein reaches an amount released of around 30 of the initial
encapsulated one while adsorbed rhBMP-2 despite its surface distribution is three times lower
However BSA shows released amounts up to 80 of the initial adsorbed ones In all cases error bars
correspond to the standard deviations from three independent experiments Under these conditions
the growth factor encapsulated in NP-BMP2 presents a release pattern similar to that previously
found with the same formulation but using lysozyme as the protein [11] Poloxamer in the water
phase of the synthesis process can be key in modulating both specific and unspecific interfacial
protein interactions [50] Thus the relation between proteinndashpolymer interaction and protein
diffusion appears to be well balanced preventing an excessive initial burst and simultaneously
maintaining the needed protein flux to release around a third of the encapsulated rhBMP-2 in 7 days
Although an excessive initial burst has been widely reported for PLGA NPs related with protein
molecules close to the surface [6] this situation did not appear for the NP-BMP2 system this being
consistent with the electrokinetic behavior that did not show the presence of protein near surface
The literature offers some examples with reduced short-term release of BMP-2 using more
hydrophilic PLGA-PEG co-polymers [16] or a different synthesis process [25]
(A) (B)
Figure 5 (A) Cumulative release of rhBMP-2 for NP-BMP2 (black square) and NP-BSA-BMP2 (red
circle) systems and cumulative release of BSA for NP-BSA-BMP2 (blue triangle) system incubated
for different times at 37 degC in saline phosphate buffer (pH 74) (B) SDS-PAGE analysis under reducing
0 20 40 60 80 100 120 140 160 180
0
20
40
60
80
100
Cum
ulat
ive
rele
ase
()
Time (hours)
Figure 5 (A) Cumulative release of rhBMP-2 for NP-BMP2 (black square) and NP-BSA-BMP2 (redcircle) systems and cumulative release of BSA for NP-BSA-BMP2 (blue triangle) system incubated fordicrarrerent times at 37 C in saline phosphate bucrarrer (pH 74) (B) SDS-PAGE analysis under reducingconditions of solid fraction of NP-BSA-BMP2 after release at dicrarrerent times where the number of eachlane corresponds to the time in hours
33 Biological Activity and Interactions
331 Cell Migration
Cell migration is the first and necessary step in tissue regeneration [52] Thus a regenerativeagent must accelerate cell migration or at least not interfere with it In the present study we found nodicrarrerences between the groups doses and control in terms of closure of a scratched area (ANOVAwith Tukey multiple comparisons test) (Figure 6) In contrast to our findings previously publisheddata suggests a positive ecrarrect of BMP-2 on cell migration [5354] However in those studies the dosesapplied and the cell types were dicrarrerent than in the current experiments We used lower doses ofBMP-2 in order to test whether even at low dosages BMP-2 could still provide benefits if protectedin a nanoparticle system As mentioned we demonstrated no negative ecrarrect of the system on cellmigration Our results nonetheless support the idea that BMP-2 activity is mediated by the activation ofthe phosphoinositide 3-kinase (PI3K) pathway a common group of signaling molecules that participatein several process with BMP-2 and other molecules [2654] It should also be mentioned that thetimeframe of a migration assay is short Thus the potential advantages of a controlled-release systemas the one under study might be limited That is the release of BMP-2 from the nanoparticles asdemonstrated in Figure 5 is limited to the first 48 h Thus a sustained positive ecrarrect on migrationactivity over time could be hypothesized
Pharmaceutics 2019 11 388 12 of 18Pharmaceutics 2019 11 x 12 of 18
Figure 6 Migration assay Percentage of scratched area closure at 24 and 48 h on different groups and doses
332 Cell Proliferation
Proliferation is another of the cell activities required for tissue regeneration However this property must be balanced with both migration and differentiation and not all three characteristics increase at the same time and with the same ratios [55] In fact reportedly when a dose of BMP-2 induces higher proliferation it decreases differentiation [56] This property has been extensively analyzed but discrepancies can still be detected in the literature Therefore Kim et al analyzed different doses of BMP-2 and its effect on cell proliferation and apoptosis It was confirmed in vitro that high doses but still lower than those used clinically reduce cell proliferation and increase apoptosis [57] This should be avoided We have found that although free BMP-2 does not induce higher proliferation than the control at any of the doses applied nor time points (ANOVA with Tukey multiple comparisons test) the same amount of BMP-2 encapsulated or adsorbed onto PLGA nanoparticles boosts proliferation this being statistically significant when using a dose of 25 ngmL or higher (ANOVA with Tukey multiple comparisons test) (Figure 7) These dosages are still lower than those suggested in previous studies Apart from that difference a positive effect on proliferation was still achieved Moreover following the release pattern from Figure 5 more BMP-2 is expected to be released over time beyond the 7-day time frame Thus a sustained induction effect could be expected as well until full confluency of the cell culture
0
10
20
30
40
50
60
70
80
90
100
0 h 24 h 48 h
o
f scr
atch
ed a
rea
Time point
Control
BMP2-125 ngmL
BMP2-25 ngmL
BMP2-5 ngmL
NP-BMP2-125 ngmL
NP-BMP2-25 ngmL
NP-BMP2-5 ngmL
NP-BSA-BMP2-125 ngmL
NP-BSA-BMP2-25 ngmL
NP-BSA-BMP2-5 ngmL
Figure 6 Migration assay Percentage of scratched area closure at 24 and 48 h on dicrarrerent groupsand doses
332 Cell Proliferation
Proliferation is another of the cell activities required for tissue regeneration However this propertymust be balanced with both migration and dicrarrerentiation and not all three characteristics increaseat the same time and with the same ratios [55] In fact reportedly when a dose of BMP-2 induceshigher proliferation it decreases dicrarrerentiation [56] This property has been extensively analyzed butdiscrepancies can still be detected in the literature Therefore Kim et al analyzed dicrarrerent doses ofBMP-2 and its ecrarrect on cell proliferation and apoptosis It was confirmed in vitro that high doses butstill lower than those used clinically reduce cell proliferation and increase apoptosis [57] This shouldbe avoided We have found that although free BMP-2 does not induce higher proliferation than thecontrol at any of the doses applied nor time points (ANOVA with Tukey multiple comparisons test)the same amount of BMP-2 encapsulated or adsorbed onto PLGA nanoparticles boosts proliferationthis being statistically significant when using a dose of 25 ngmL or higher (ANOVA with Tukeymultiple comparisons test) (Figure 7) These dosages are still lower than those suggested in previousstudies Apart from that dicrarrerence a positive ecrarrect on proliferation was still achieved Moreoverfollowing the release pattern from Figure 5 more BMP-2 is expected to be released over time beyondthe 7-day time frame Thus a sustained induction ecrarrect could be expected as well until full confluencyof the cell culture
Pharmaceutics 2019 11 388 13 of 18Pharmaceutics 2019 11 x 13 of 18
Figure 7 Proliferation of human mesenchymal stromal cells (MSCs) as measured by sulphorhodamine (SRB) absorbance Results were normalized to T0 in each group
333 Osteogenic Differentiation
It has been confirmed that cell differentiation induced by BMP-2 needs the presence of permissive osteoinductive components Particularly β-glycerophosphate has been shown to exert a synergistic effect with BMP-2 in inducing cell differentiation [56] Thus to test for osteogenic differentiation we analyzed the expression of ALP mRNA Maximum ALP activity was found to occur 10 days after stimulation with PLGA-based microparticles containing BMP-2 in co-encapsulation with human serum albumin [16] Although other tests could have been used to reinforce our findings ALP is known to modulate the deposition of mineralized nodules thus indicating osteoblastic activity For all of this we supplemented the differentiation media with β-glycerophosphate and either free BMP-2 NP-BMP2 or NP-BSA-BMP2 for 4 and 7 days so that we could capture the early dynamics of the expression of the gene In our study we identified an increase in the expression of ALP in all groups from day 4 to day 7 (Figure 8) Although ALP at day 7 in the BMP-2 group appears to be higher than for the other two groups the change did not prove significant In fact differences between groups were not statistically significant within any time period Noteworthy though the increase was not significant within the BMP-2 group (p = 0141 Studentrsquos t test) but it was significant within the other two groups (p = 0025 and p = 0003 NP-BMP2 and NP-BSA-BMP2 groups respectively) This again could be taken as a confirmation of the sustained release of the protein from the nanoparticle system beyond the earlier time points
This and both the migration and proliferation studies described below lead us to confirm that the system proposed can maintain a proper release of BMP-2 over time sustaining a positive effect on cell migration and proliferation with initial reduced doses of BMP-2 The fact that the excessive initial burst is prevented is important for the application of this nanotechnology in bone regeneration as in dentistry In this way the negative effects of initial high doses of BMP-2 are avoided at the same time as the molecule is protected from denaturalization inside the NP Thus the regenerator effects are maintained over time
T0 T1 T2 T3 T4 T6 T70
2
4
6
Time point
Nor
mal
ized
Abs
orba
nce
NP-BSA-BMP2-125 ngmL
NP-BSA-BMP2-25 ngmL
NP-BSA-BMP2-5 ngmL
NP-BMP2-125 ngmL
NP-BMP2-25 ngmL
NP-BMP2-5 ngmL
BMP2-125 ngmL
BMP2-25 ngmL
BMP2-5 ngmL
Control
Figure 7 Proliferation of human mesenchymal stromal cells (MSCs) as measured by sulphorhodamine(SRB) absorbance Results were normalized to T0 in each group
333 Osteogenic Dicrarrerentiation
It has been confirmed that cell dicrarrerentiation induced by BMP-2 needs the presence of permissiveosteoinductive components Particularly -glycerophosphate has been shown to exert a synergisticecrarrect with BMP-2 in inducing cell dicrarrerentiation [56] Thus to test for osteogenic dicrarrerentiation weanalyzed the expression of ALP mRNA Maximum ALP activity was found to occur 10 days afterstimulation with PLGA-based microparticles containing BMP-2 in co-encapsulation with human serumalbumin [16] Although other tests could have been used to reinforce our findings ALP is known tomodulate the deposition of mineralized nodules thus indicating osteoblastic activity For all of this wesupplemented the dicrarrerentiation media with -glycerophosphate and either free BMP-2 NP-BMP2 orNP-BSA-BMP2 for 4 and 7 days so that we could capture the early dynamics of the expression of thegene In our study we identified an increase in the expression of ALP in all groups from day 4 to day 7(Figure 8) Although ALP at day 7 in the BMP-2 group appears to be higher than for the other twogroups the change did not prove significant In fact dicrarrerences between groups were not statisticallysignificant within any time period Noteworthy though the increase was not significant within theBMP-2 group (p = 0141 Studentrsquos t test) but it was significant within the other two groups (p = 0025and p = 0003 NP-BMP2 and NP-BSA-BMP2 groups respectively) This again could be taken as aconfirmation of the sustained release of the protein from the nanoparticle system beyond the earliertime points
This and both the migration and proliferation studies described below lead us to confirm that thesystem proposed can maintain a proper release of BMP-2 over time sustaining a positive ecrarrect on cellmigration and proliferation with initial reduced doses of BMP-2 The fact that the excessive initialburst is prevented is important for the application of this nanotechnology in bone regeneration asin dentistry In this way the negative ecrarrects of initial high doses of BMP-2 are avoided at the sametime as the molecule is protected from denaturalization inside the NP Thus the regenerator ecrarrects aremaintained over time
Pharmaceutics 2019 11 388 14 of 18
14 of 18
4 Conclusions
In this work a delivery PLGA-nanosystem previously developed for model proteins was chosen as the reference system to carry and deliver the growth factor BMP-2 This NP system with a dual size distribution was developed following a double-emulsion formulation in which the process and the components used were optimized to reach the appropriate colloidal and biological behavior Encapsulation and adsorption are two different processes to load BMP-2 in PLGA NPs Both were tested to elucidate the factors controlling them and their influence in the physico-chemical and biological properties of nanosystems We verified that proteinndashpolymer specific interactions have a major role in the way that protein molecules are carried and delivered from NPs In vitro experiments showed that BMP-2-loaded PLGA NPs are the nanocarriers with the best release profile over the short-term without an initial burst and with moderate and sustained release of active protein before the onset of polymer degradation Therefore the biological activity is positive with no negative interaction with migration or proliferation but rather the induction of cell differentiation through the expression of ALP
Supplementary Materials The following are available online at wwwmdpicomxxxs1 Figure S1 Scheme of the formulation of NP-BMP2 Figure S2 Scheme of the protein adsorption process for NP-BSA-BMP2 Video S1 NTA experiments for NP-BMP2 Video S2 NTA experiments for empty NPs
Author Contributions Conceptualization JMP-G and PG-M methodology JMP-G ABJ-R and MP-M investigation TdC-S IO-O JMP-G ABJ-R and MP-M resources ABJ-R PG-M FO-R writingmdashoriginal draft preparation JMP-G and MP-M writingmdashreview and editing JMP-G MP-M ABJ-R TdC-S supervision JMP-G PG-M and FO-R funding acquisition ABJ-R and PG-M
Funding This research was funded by the Consejeriacutea de Economiacutea Innovacioacuten Ciencia y Empleo de la Junta de Andaluciacutea (Spain) through research groups FQM-115 and CTS-1028 by the following research project MAT2013-43922-RmdashEuropean FEDER support includedmdash(MICINN Spain) and by MIS Ibeacuterica SL
Acknowledgments The authors wish to express their appreciation for the technical support to D Dariacuteo Abril-Garciacutea
Conflicts of Interest The authors declare no conflict of interest
Figure 8 Relative fold change in the expression of ALP mRNA (control group BMP2 at 4 days) =Statistical significance of the comparison over time (p = 0025 and p = 0003 Studentrsquos t test NP-BMP2and NP-BSA-BMP2 groups)
4 Conclusions
In this work a delivery PLGA-nanosystem previously developed for model proteins was chosenas the reference system to carry and deliver the growth factor BMP-2 This NP system with a dualsize distribution was developed following a double-emulsion formulation in which the processand the components used were optimized to reach the appropriate colloidal and biological behaviorEncapsulation and adsorption are two dicrarrerent processes to load BMP-2 in PLGA NPs Both were testedto elucidate the factors controlling them and their influence in the physico-chemical and biologicalproperties of nanosystems We verified that proteinndashpolymer specific interactions have a major role inthe way that protein molecules are carried and delivered from NPs In vitro experiments showed thatBMP-2-loaded PLGA NPs are the nanocarriers with the best release profile over the short-term withoutan initial burst and with moderate and sustained release of active protein before the onset of polymerdegradation Therefore the biological activity is positive with no negative interaction with migrationor proliferation but rather the induction of cell dicrarrerentiation through the expression of ALP
Supplementary Materials The following are available online at httpwwwmdpicom1999-4923118388s1Figure S1 Scheme of the formulation of NP-BMP2 Figure S2 Scheme of the protein adsorption process forNP-BSA-BMP2 Video S1 NTA experiments for NP-BMP2 Video S2 NTA experiments for empty NPs
Author Contributions Conceptualization JMP-G and PG-M methodology JMP-G ABJ-R andMP-M investigation TdC-S IO-O JMP-G ABJ-R and MP-M resources ABJ-R PG-M FO-Rwritingmdashoriginal draft preparation JMP-G and MP-M writingmdashreview and editing JMP-G MP-MABJ-R TdC-S supervision JMP-G PG-M and FO-R funding acquisition ABJ-R and PG-M
Funding This research was funded by the Consejeriacutea de Economiacutea Innovacioacuten Ciencia y Empleo de la Juntade Andaluciacutea (Spain) through research groups FQM-115 and CTS-1028 by the following research projectMAT2013-43922-RmdashEuropean FEDER support includedmdash(MICINN Spain) and by MIS Ibeacuterica SL
Acknowledgments The authors wish to express their appreciation for the technical support to D DariacuteoAbril-Garciacutea
Conflicts of Interest The authors declare no conflict of interest
Pharmaceutics 2019 11 388 15 of 18
References
1 Van Rijt S Habibovic P Enhancing regenerative approaches with nanoparticles J R Soc Interface 2017 14[CrossRef]
2 Kumar B Jalodia K Kumar P Gautam HK Recent advances in nanoparticle-mediated drug delivery JDrug Deliv Sci Technol 2017 41 260ndash268 [CrossRef]
3 Mir M Ahmed N Rehman AUR Recent applications of PLGA based nanostructures in drug deliveryColloids Surf B Biointerfaces 2017 159 217ndash231 [CrossRef] [PubMed]
4 Jana S Jana S Natural polymeric biodegradable nanoblend for macromolecules delivery In RecentDevelopments in Polymer Macro Micro and Nano Blends Woodhead Publishing Cambridge UK 2017pp 289ndash312 ISBN 9780081004081
5 Danhier F Ansorena E Silva JM Coco R Le Breton A Preacuteat V PLGA-based nanoparticles Anoverview of biomedical applications J Control Release 2012 161 505ndash522 [CrossRef] [PubMed]
6 Ding D Zhu Q Recent advances of PLGA micronanoparticles for the delivery of biomacromoleculartherapeutics Mater Sci Eng C 2018 92 1041ndash1060 [CrossRef] [PubMed]
7 Arias JL Unciti-Broceta JD Maceira J del Castillo T Hernaacutendez-Quero J Magez SSoriano M Garciacutea-Salcedo JA Nanobody conjugated PLGA nanoparticles for active targeting of AfricanTrypanosomiasis J Control Release 2015 197 190ndash198 [CrossRef]
8 Giteau A Venier-Julienne MC Aubert-Poueumlssel A Benoit JP How to achieve sustained and completeprotein release from PLGA-based microparticles Int J Pharm 2008 350 14ndash26 [CrossRef]
9 Fredenberg S Wahlgren M Reslow M Axelsson A The mechanisms of drug release inpoly(lactic-co-glycolic acid)-based drug delivery systemsmdashA review Int J Pharm 2011 415 34ndash52[CrossRef]
10 White LJ Kirby GTS Cox HC Qodratnama R Qutachi O Rose FRAJ Shakeshecrarr KM Acceleratingprotein release from microparticles for regenerative medicine applications Mater Sci Eng C 2013 332578ndash2583 [CrossRef]
11 Ortega-Oller I del Castillo-Santaella T Padial-Molina M Galindo-Moreno P Joacutedar-Reyes ABPeula-Garciacutea JM Dual delivery nanosystem for biomolecules Formulation characterization and in vitrorelease Colloids Surf B Biointerfaces 2017 159 586ndash595 [CrossRef]
12 McClements DJ Encapsulation protection and delivery of bioactive proteins and peptides usingnanoparticle and microparticle systems A review Adv Colloid Interface Sci 2018 253 1ndash22 [CrossRef]
13 Ortega-Oller I Padial-Molina M Galindo-Moreno P OrsquoValle F Joacutedar-Reyes AB Peula-Garciacutea JMBone Regeneration from PLGA Micro-Nanoparticles BioMed Res Int 2015 2015 1ndash18 [CrossRef] [PubMed]
14 Bapat RA Joshi CP Bapat P Chaubal TV Pandurangappa R Jnanendrappa N Gorain B Khurana SKesharwani P The use of nanoparticles as biomaterials in dentistry Drug Discov Today 2019 24 85ndash98[CrossRef]
15 Ji Y Xu GP Zhang ZP Xia JJ Yan JL Pan SH BMP-2PLGA delayed-release microspheres compositegraft selection of bone particulate diameters and prevention of aseptic inflammation for bone tissueengineering Ann BioMed Eng 2010 38 632ndash639 [CrossRef] [PubMed]
16 Kirby GTS White LJ Rahman CV Cox HC Qutachi O Rose FRAJ Hutmacher DWShakeshecrarr KM Woodrucrarr MA PLGA-Based Microparticles for the Sustained Release of BMP-2 Polymers2011 3 571ndash586 [CrossRef]
17 Qutachi O Shakeshecrarr KM Buttery LDK Delivery of definable number of drug or growth factor loadedpoly(dl-lactic acid-co-glycolic acid) microparticles within human embryonic stem cell derived aggregates JControl Release 2013 168 18ndash27 [CrossRef] [PubMed]
18 Wang Y Wei Y Zhang X Xu M Liu F Ma Q Cai Q Deng X PLGAPDLLA core-shell submicronspheres sequential release system Preparation characterization and promotion of bone regeneration in vitroand in vivo Chem Eng J 2015 273 490ndash501 [CrossRef]
Pharmaceutics 2019 11 388 16 of 18
19 Zhang H-X Zhang X-P Xiao G-Y Hou -Y Cheng L Si M Wang S-S Li Y-H Nie L In vitroand in vivo evaluation of calcium phosphate composite scacrarrolds containing BMP-VEGF loaded PLGAmicrospheres for the treatment of avascular necrosis of the femoral head Mater Sci Eng C 2016 60 298ndash307[CrossRef]
20 Begam H Nandi SK Kundu B Chanda A Strategies for delivering bone morphogenetic protein forbone healing Mater Sci Eng C 2017 70 856ndash869 [CrossRef]
21 Balmayor ER Feichtinger GA Azevedo HS Van Griensven M Reis RL Starch-poly--caprolactonemicroparticles reduce the needed amount of BMP-2 Clin Orthop Relat Res 2009 467 3138ndash3148 [CrossRef]
22 Xu Y Kim CS Saylor DM Koo D Polymer degradation and drug delivery in PLGA-based drugndashpolymerapplications A review of experiments and theories J BioMed Mater Res Part B Appl Biomater 2017 1051692ndash1716 [CrossRef] [PubMed]
23 Padial-Molina M de Buitrago JG Sainz-Urruela R Abril-Garcia D Anderson P OrsquoValle FGalindo-Moreno P Expression of Musashi-1 during osteogenic dicrarrerentiation of oral MSC An in vitro studyInt J Mol Sci 2019 20 2171 [CrossRef] [PubMed]
24 DrsquoAngelo I Garcia-Fuentes M Parajoacute Y Welle A Vaacutentus T Horvaacuteth A Boumlkoumlnyi G Keacuteri GAlonso MJ Nanoparticles based on PLGApoloxamer blends for the delivery of proangiogenic growthfactors Mol Pharm 2010 7 1724ndash1733 [CrossRef] [PubMed]
25 Chang H-C Yang C Feng F Lin F-H Wang C-H Chang P-C Bone morphogeneticprotein-2 loaded poly(DL-lactide-co-glycolide) microspheres enhance osteogenic potential ofgelatinhydroxyapatite-tricalcium phosphate cryogel composite for alveolar ridge augmentation JFormos Med Assoc 2017 116 973ndash981 [CrossRef] [PubMed]
26 Padial-Molina M Volk SL Rios HF Periostin increases migration and proliferation of humanperiodontal ligament fibroblasts challenged by tumor necrosis factor -crarr and Porphyromonas gingivalislipopolysaccharides J Periodontal Res 2014 49 405ndash414 [CrossRef] [PubMed]
27 Liang C-C Park AY Guan J-L In vitro scratch assay A convenient and inexpensive method for analysisof cell migration in vitro Nat Protoc 2007 2 329ndash333 [CrossRef]
28 Houghton P Fang R Techatanawat I Steventon G Hylands PJ Lee CC The sulphorhodamine (SRB)assay and other approaches to testing plant extracts and derived compounds for activities related to reputedanticancer activity Methods 2007 42 377ndash387 [CrossRef]
29 Iqbal M Zafar N Fessi H Elaissari A Double emulsion solvent evaporation techniques used for drugencapsulation Int J Pharm 2015 496 173ndash190 [CrossRef]
30 Saacutenchez-Moreno P Ortega-Vinuesa JL Boulaiz H Marchal JA Peula-Garciacutea JM Synthesis andcharacterization of lipid immuno-nanocapsules for directed drug delivery Selective antitumor activityagainst HER2 positive breast-cancer cells Biomacromolecules 2013 14 4248ndash4259 [CrossRef]
31 Lochmann A Nitzsche H von Einem S Schwarz E Maumlder K The influence of covalently linked andfree polyethylene glycol on the structural and release properties of rhBMP-2 loaded microspheres J ControlRelease 2010 147 92ndash100 [CrossRef]
32 Kempen DHR Lu L Hecrarreran TE Creemers LB Maran A Classic KL Dhert WJA Yaszemski MJRetention of in vitro and in vivo BMP-2 bioactivities in sustained delivery vehicles for bone tissue engineeringBiomaterials 2008 29 3245ndash3252 [CrossRef]
33 Santander-Ortega MJ Csaba N Gonzaacutelez L Bastos-Gonzaacutelez D Ortega-Vinuesa JL Alonso MJProtein-loaded PLGAndashPEO blend nanoparticles Encapsulation release and degradation characteristicsColloid Polym Sci 2010 288 141ndash150 [CrossRef]
34 Chung YI Ahn KM Jeon SH Lee SY Lee JH Tae G Enhanced bone regeneration with BMP-2loaded functional nanoparticle-hydrogel complex J Control Release 2007 121 91ndash99 [CrossRef]
35 La W-G Kang S-W Yang HS Bhang SH Lee SH Park J-H Kim B-S The Ecacy of BoneMorphogenetic Protein-2 Depends on Its Mode of Delivery Artif Organs 2010 34 1150ndash1153 [CrossRef]
36 Fu Y Du L Wang Q Liao W Jin Y Dong A Chen C Li Z In vitro sustained release of recombinanthuman bone morphogenetic protein-2 microspheres embedded in thermosensitive hydrogels Die Pharm2012 67 299ndash303 [CrossRef]
Pharmaceutics 2019 11 388 17 of 18
37 Rahman CV Ben-David D Dhillon A Kuhn G Gould TWA Muumlller R Rose FRAJ Shakeshecrarr KMLivne E Controlled release of BMP-2 from a sintered polymer scacrarrold enhances bone repair in a mousecalvarial defect model J Tissue Eng Regen Med 2014 8 59ndash66 [CrossRef]
38 Pakulska MM Elliott Donaghue I Obermeyer JM Tuladhar a McLaughlin CK Shendruk TNShoichet MS Encapsulation-free controlled release Electrostatic adsorption eliminates the need for proteinencapsulation in PLGA nanoparticles Sci Adv 2016 2 e1600519 [CrossRef]
39 Fu C Yang X Tan S Song L Enhancing Cell Proliferation and Osteogenic Dicrarrerentiation of MC3T3-E1Pre-osteoblasts by BMP-2 Delivery in Graphene Oxide-Incorporated PLGAHA Biodegradable MicrocarriersSci Rep 2017 7 12549 [CrossRef]
40 Peula JM de las Nieves FJ Adsorption of monomeric bovine serum albumin on sulfonated polystyrenemodel colloids 1 Adsorption isotherms and ecrarrect of the surface charge density Colloids Surf A PhysicochemEng Asp 1993 77 199ndash208 [CrossRef]
41 Peula JM de las Nieves FJ Adsorption of monomeric bovine serum albumin on sulfonated polystyrenemodel colloids 3 Colloidal stability of latexmdashProtein complexes Colloids Surf A Physicochem Eng Asp1994 90 55ndash62 [CrossRef]
42 Peula JM Hidalgo-Alvarez R De Las Nieves FJ Coadsorption of IgG and BSA onto sulfonated polystyrenelatex I Sequential and competitive coadsorption isotherms J Biomater Sci Polym Ed 1996 7 231ndash240[CrossRef]
43 Siafaka PI Uumlstuumlndag Okur N Karavas E Bikiaris DN Surface modified multifunctional and stimuliresponsive nanoparticles for drug targeting Current status and uses Int J Mol Sci 2016 17 1440[CrossRef]
44 Peula-Garciacutea JM Hidalgo-Alvarez R De Las Nieves FJ Colloid stability and electrokinetic characterizationof polymer colloids prepared by dicrarrerent methods Colloids Surf A Physicochem Eng Asp 1997 127 19ndash24[CrossRef]
45 Santander-Ortega MJ Lozano-Loacutepez MV Bastos-Gonzaacutelez D Peula-Garciacutea JM Ortega-Vinuesa JLNovel core-shell lipid-chitosan and lipid-poloxamer nanocapsules Stability by hydration forces ColloidPolym Sci 2010 288 159ndash172 [CrossRef]
46 Peula-Garcia JM Hidaldo-Alvarez R De las Nieves FJ Protein co-adsorption on dicrarrerent polystyrenelatexes Electrokinetic characterization and colloidal stability Colloid Polym Sci 1997 275 198ndash202[CrossRef]
47 Santander-Ortega MJ Bastos-Gonzaacutelez D Ortega-Vinuesa JL Electrophoretic mobility and colloidalstability of PLGA particles coated with IgG Colloids Surf B Biointerfaces 2007 60 80ndash88 [CrossRef]
48 Peula JM Callejas J de las NIeves FJ Adsorption of Monomeric Bovine Serum Albumin on SulfonatedPolystyrene Model Colloids II Electrokinetic Characterization of Latex-Protein Complexes In SurfaceProperties of Biomaterials Butterworth and Heinemann Oxford UK 1994 pp 61ndash69
49 Sun D Ecrarrect of Zeta Potential and Particle Size on the Stability of SiO2 Nanospheres as Carrier for UltrasoundImaging Contrast Agents Int J Electrochem Sci 2016 8520ndash8529 [CrossRef]
50 del Castillo-Santaella T Peula-Garciacutea JM Maldonado-Valderrama J Joacutedar-Reyes AB Interaction ofsurfactant and protein at the OW interface and its ecrarrect on colloidal and biological properties of polymericnanocarriers Colloids Surf B Biointerfaces 2019 173 295ndash302 [CrossRef]
51 Schrier JA DeLuca PP Porous bone morphogenetic protein-2 microspheres Polymer binding and in vitrorelease AAPS PharmSciTech 2001 2 66ndash72 [CrossRef]
52 Padial-Molina M OrsquoValle F Lanis A Mesa F Dohan Ehrenfest DM Wang H-L Galindo-Moreno PClinical application of mesenchymal stem cells and novel supportive therapies for oral bone regenerationBioMed Res Int 2015 2015 [CrossRef]
53 Inai K Norris RA Hocrarrman S Markwald RR Sugi Y BMP-2 induces cell migration and periostinexpression during atrioventricular valvulogenesis Dev Biol 2008 315 383ndash396 [CrossRef]
54 Gamell C Osses N Bartrons R Ruumlckle T Camps M Rosa JL Ventura F Imamura T BMP2 inductionof actin cytoskeleton reorganization and cell migration requires PI3-kinase and Cdc42 activity J Cell Sci2008 121 3960ndash3970 [CrossRef]
55 Friedrichs M Wirsdoumlerfer F Floheacute SB Schneider S Wuelling M Vortkamp A BMP signaling balancesproliferation and dicrarrerentiation of muscle satellite cell descendants BMC Cell Biol 2011 12 26 [CrossRef]
Pharmaceutics 2019 11 388 18 of 18
56 Hrubi E Imre L Robaszkiewicz A Viraacuteg L Kereacutenyi F Nagy K Varga G Jenei A Hegeduumls CDiverse ecrarrect of BMP-2 homodimer on mesenchymal progenitors of dicrarrerent origin Hum Cell 2018 31139ndash148 [CrossRef]
57 Kim HKW Oxendine I Kamiya N High-concentration of BMP2 reduces cell proliferation and increasesapoptosis via DKK1 and SOST in human primary periosteal cells Bone 2013 54 141ndash150 [CrossRef]
copy 2019 by the authors Licensee MDPI Basel Switzerland This article is an open accessarticle distributed under the terms and conditions of the Creative Commons Attribution(CC BY) license (httpcreativecommonsorglicensesby40)
157
158
12ANEXO DE ORIGINALIDAD
3
BIO-NANOTECNOLOGIacuteA APLICADA A LA
REGENERACIOacuteN OacuteSEA MEDIANTE EL
TRANSPORTE DE BIOMOLEacuteCULAS USANDO
NANOPARTIacuteCULAS POLIMEacuteRICAS ESTUDIO
IN VITRO
por
Inmaculada Ortega Oller
Licenciada en Odontologiacutea
Directores de la Tesis
Dr D Joseacute Manuel Peula
Prof Titular de Fiacutesica Aplicada
Dr D Francisco OacuteValle Ravassa
Prof Catedraacutetico de Anatomiacutea Patoloacutegica
Dr D Pablo Galindo Moreno
Prof Catedraacutetico de Estomatologiacutea
4
A mi familia
y directores y en especial
a mi PADRE
Juan Ortega Navarro
5
Agradecimientos
Despueacutes de un apasionado y largo periacuteodo de elaboracioacuten de esta tesis doctoral hoy es el diacutea
escribo este apartado de agradecimientos para finalizar un arduo trabajo Ha sido un periacuteodo de
aprendizaje intenso no solo en el campo cientiacutefico sino tambieacuten a nivel personal que ha supuesto
un gran impacto en miacute Por tal motivo me gustariacutea agradecer a todas aquellas personas que me
han ayudado y brindado su apoyado durante este proceso
En primer lugar quiero agradecer a mis tutores
Don Jose Manuel Peula Garciacutea Profesor Titular de Fiacutesica Aplicada quien con sus
conocimientos y apoyo me guioacute a traveacutes de cada una de las etapas de este proyecto para alcanzar
los resultados que buscaba Gracias por su paciencia calma y tranquilidad para explicar
detenidamente a una odontoacuteloga todos y cada uno de los conceptos aprendidos e interiorizados
Todo mi agradecimiento por su tiempo dedicacioacuten y por haberme acogido de la manera que lo
hizo daacutendome todo su carintildeo apoyo y compresioacuten
Don Pablo Galindo Moreno Profesor Catedraacutetico de Estomatologiacutea
Un trabajo de investigacioacuten es siempre fruto de ideas proyectos y esfuerzos previos que
corresponden a otras personas y que te escogen a ti para saber llevarlas a cabo En este caso mi
maacutes sincero agradecimiento a usted con cuyo trabajo estareacute siempre en deuda y con quien he
compartido proyectos e ilusiones durante todos estos antildeos Gracias por su amabilidad para
facilitarme su tiempo y sus ideas Ha sido una fuente de paz en tiempos muy duros
6
Don Francisco OacuteValle Ravassa Profesor Catedraacutetico de Anatomiacutea Patoloacutegica por su
orientacioacuten atencioacuten a mis consultas y por sus valiosas sugerencias en momentos de duda asiacute
como por su completa disponibilidad siempre que lo he necesitado
Tambien quiero agradecer a Mis Iberica SL y a la Consejeriacutea de Economiacutea Innovacioacuten
Educacioacuten Ciencia y Empleo de la Junta de Andaluciacutea por brindarme todos los recursos y
herramientas que fueron necesarios para llevar a cabo el proceso de investigacioacuten No hubiese
podido arribar a estos resultados sin su incondicional ayuda
Un trabajo de investigacioacuten es tambieacuten fruto del reconocimiento y del apoyo vital que nos
ofrecen las personas que nos estiman sin el cual no tendriacuteamos la fuerza y energiacutea que nos anima
a crecer como personas y como profesionales este es el caso del Dr Don Miguel Padial y de las
Dras Dontildea Azahara Rata-Aguilar Dontildea Ana Jodar y DontildeaTeresa Del Castillo Gracias por
vuestra cercana visioacuten de todo Tambien queriacutea dedicar unas palabras a un buen amigo Javier
Vidao quien con sus conocimientos informaacuteticos me ha ayudado con la elaboracioacuten de este
documento de tesis Gracias
Quiero agradecer tambieacuten a todos mis compantildeeros y amigos por hacer feliz mi dia a dia y a mi
familia por apoyarme auacuten cuando mis aacutenimos decaiacutean A mis hermanos Juan Javier y Dorothy y
a mi madre que siempre estuvieron ahiacute para darme palabras de apoyo y un abrazo reconfortante
para renovar energiacuteas
Gracias a mi pareja por su paciencia comprensioacuten y solidaridad con este proyecto por el
tiempo que me ha concedido un tiempo robado al disfrute conjunto que ha respetado y valorado
Te agradezco la esperanza que me has brindado en los momentos y situaciones mas tormentosas
de mi vida
7
Por uacuteltimo dedico con todo mi corazoacuten mi tesis doctoral a mi PADRE a quien perdimos
recientemente pues sin eacutel no habriacutea logrado nada de esto gracias por haberme forjado como la
persona que hoy soy
Muchos de mis logros se los debo a eacutel quien me educoacute con firmeza pero a la vez sabiendo
darme la libertad suficiente para permitirme evolucionar por mi misma como persona
motivandome constantemente a alcanzar mis metas Fue mi referente en la vida y quien me dioacute el
uacuteltimo y maacutes grande de los aprendizajes dejando en miacute un gran espiacuteritu de lucha sacrificio
esfuerzo y sabiduriacutea ante la maacutes difiacutecil situacioacuten planteable Por todo ello este trabajo te lo dedico
a ti alliacute donde esteacutes espero que seas feliz y puedas disfrutar de los logros conseguidos aquiacute
A todos muchas gracias
8
RESUMEN
REGENERACIOacuteN OacuteSEA A PARTIR DE NANOMICROPARTICULAS DE PLGA
CARGADAS DE BMP-2
El aacutecido poli-laacutectico-co-glicoacutelico (PLGA) es uno de los poliacutemeros sinteacuteticos maacutes ampliamente
utilizados para el desarrollo de sistemas de administracioacuten de faacutermacos y biomoleacuteculas
terapeacuteuticas asi como componente principal en aplicaciones de ingenieriacutea de tejidos Sus
propiedades y versatilidad le permiten ser un poliacutemero de referencia en la fabricacioacuten de
nanopartiacuteculas y micropartiacuteculas para encapsular y liberar una amplia variedad de moleacuteculas
hidrofoacutebicas e hidrofiacutelicas Ademaacutes sus propiedades de biodegradabilidad y biocompatibilidad
hacen del mismo un candidato idoacuteneo para encapsular biomoleacuteculas como proteiacutenas o aacutecidos
nucleicos permitiendo su liberacioacuten de forma controlada
Este trabajo se centra en el uso de nanopartiacuteculas (NP) de PLGA como un sistema de entrega
de uno de los factores de crecimiento maacutes comuacutenmente utilizados en la ingenieriacutea del tejido oacuteseo
la proteiacutena morfogeneacutetica oacutesea 2 (BMP2) Por lo tanto examinamos todos los requisitos
necesarios para alcanzar una correcta encapsulacioacuten y una liberacioacuten controlada y sostenida de
BMP2 utilizando partiacuteculas de PLGA como componente principal discutiendo todos los
problemas y soluciones que hemos encontrado para el desarrollo adecuado de este sistema con un
gran potencial en el proceso de diferenciacioacuten celular y proliferacioacuten bajo el punto de vista de la
regeneracioacuten oacutesea
Hemos desarrollado y optimizando dos meacutetodos de formulacioacuten diferentes para obtener NP de
PLGA cargadas con una proteiacutena modelo con actividad enzimaacutetica como la lisozima que posee
caracteriacutesticas similares a la BMP2 Estas formulaciones se basan en una teacutecnica de doble
emulsioacuten con evaporacioacuten de solvente (agua aceite agua WOW) Se diferencian
principalmente en la fase en la que se agrega el surfactante (Pluronicreg F68) agua (W-F68) o
9
aceite (O-F68) Este surfactante polimeacuterico no ioacutenico puede modular una serie de propiedades del
nanosistema transportador en el que se integra reduciendo el tamantildeo de las NPs incrementando
su estabilidad coloidal y facilitando la proteccioacuten de la biomoleacutecula encapsulada Ademaacutes gracias
a su disposicioacuten superficial y la hidrofilidad de sus colas polares se reduce la interaccioacuten con el
sistema fagociacutetico mononuclear con una mejora de la biodistribucioacuten al aumentar su tiempo de
circulacioacuten despueacutes de una administracioacuten intravenosa en un organismo vivo
Analizamos las propiedades coloidales de estos sistemas usando diferentes teacutecnicas
experimentales (morfologiacutea por SEM y STEM tamantildeo hidrodinaacutemico por DLS y NTA
movilidad electroforeacutetica estabilidad temporal en diferentes medios) asiacute como la encapsulacioacuten
patroacuten de liberacioacuten y bioactividad de la lisozima Asimismo realizamos una caracterizacioacuten
interfacial de la interaccioacuten surfactante-proteiacutena en la primera emulsioacuten agua-aceite para cada
procedimiento de formulacioacuten mediante el anaacutelisis de la tensioacuten superficial y la elasticidad
Finalmente examinamos la captacioacuten celular por ceacutelulas estromales mesenquimaacuteticas humanas y
la citotoxicidad para ambos nanosistemas
Mediante las dos formulaciones O-F68 y W-F68 se obtienen NPs soacutelidas de morfologiacutea
esfeacuterica si bien en un caso el sistema presenta monodispersidad con diaacutemetros alrededor de 120
nm (O-F68) en el otro se obtiene un nanosistema polidisperso con diaacutemetros de partiacutecula
comprendidos entre 100 y 500 nm (W-F68) Como resultado maacutes relevante observamos que la
eficacia de encapsulacioacuten la liberacioacuten y la bioactividad de la lisozima se han mantenido mejor
con el meacutetodo de formulacioacuten W-F68 En este caso dada la heterogeneidad de tamantildeos se podriacutea
hablar de un prometedor sistema multimodal para encapsular proteiacutenas con una fuerte actividad
bioloacutegica que permita una ldquoentrega dualrdquo a nivel extra- e intracelular facilitando la actividad
proteica en la superficie celular y en el citoplasma
Tras desarrollar y optimizar el meacutetodo de siacutentesis para las NPs de PLGA cargadas de lisozima
tratamos de adaptar la formulacioacuten para conseguir la encapsulacioacuten de la proteiacutena terapeacuteutica
BMP-2 Asiacute basaacutendonos en los resultados obtenidos con la lisozima se ha optado por usar el
10
procedimento de siacutentesis W-F68 para favorecer la proteccioacuten de las moleacuteculas proteicas y su
actividad bioloacutegica Con esta formulacioacuten se han obtenido con buena reproducibilidad NPs
esfeacutericas con el tamantildeo multimodal referido anteriormente entre 100 y 500 nm que posibilitaraacuten
el suministro extra- e intracelular Ademaacutes de NPs con BMP2 encapsulada obtenemos un
nanosistema en el que la BMP2 no estaacute encapsulada sino co-adsorbida superficialmente junto a
una proteiacutena estabilizadora como la albuacutemina de suero bovino De nuevo se lleva a cabo una
completa caracterizacioacuten fisico-quiacutemica y bioloacutegica de ambos sistemas de NPs analizando las
propiedades indicadas previamente esto es morfologiacutea y tamantildeo carga superficial estabilidad
coloidal y temporal encapsulacioacuten y patroacuten de liberacioacuten Es conocido que la cineacutetica de
liberacioacuten en los sistemas polimeacutericos basados en PLGA dependen en gran medida de la
degradacioacuten hidroliacutetica del poliacutemero Sin embargo la liberacioacuten a tiempos cortos estaacute influenciada
por otros procesos fiacutesicos y es crucial evitar una descarga inicial excesiva sobre todo si se quiere
optimizar la aplicacioacuten de esta nanotecnologiacutea en procesos de regeneracioacuten oacutesea muy importantes
en odontologiacutea En consecuencia hemos incidido en el anaacutelisis del patroacuten de liberacioacuten de la
BMP2 a tiempos cortos utilizando diferentes teacutecnicas y comparando el comportamiento de los dos
sistemas de NPs con la proteiacutena encapsulada o adsorbida superficialmente
Finalmente se ha analizado la actividad bioloacutegica de las NPs cargadas con BMP2 mediante
estudios in vitro de proliferacioacuten celular migracioacuten y diferenciacioacuten osteogeacutenica usando para ello
ceacutelulas estromales mesenquimales obtenidas a partir de hueso alveolar humano (ABSC) En base
a todo esto se puede confirmar que las NPs con BMP2 encapsuladas presentan un patroacuten de
liberacioacuten adecuado a corto plazo manteniendo un suministro proteico sostenido y una actividad
bioloacutegica adecuada para dosis iniciales de BMP2 muy reducidas
11
SUMMARY
BONE REGENERATION FROM PLGA NANOMICROPARTICLES LOADED
WITH BMP-2
Poly-lactic-co-glycolic acid (PLGA) is one of the most widely used synthetic polymers for the
development of drug delivery systems and therapeutic biomolecules and as a component of tissue
engineering applications Its properties and versatility allow it to be a reference polymer in the
manufacture of nanoparticles and microparticles to encapsulate and release a wide variety of
hydrophobic and hydrophilic molecules Furthermore its biodegradability and biocompatibility
properties make it an ideal candidate for encapsulating biomolecules such as proteins or nucleic
acids that can be released in a controlled manner This work focuses on the use of PLGA
nanoparticles (NP) as a delivery system for one of the most commonly used growth factors in
bone tissue engineering bone morphogenetic protein 2 (BMP2) Therefore we examine all the
necessary requirements to achieve a correct encapsulation and a controlled and sustained release
of BMP2 using PLGA particles as the main component discussing all the problems and solutions
that we have found for the proper development of this system with great potential in the process
of cell differentiation and proliferation from the point of view of bone regeneration We have
developed and optimized two different formulation methods to obtain PLGA NP loaded with a
model protein with enzymatic activity such as lysozyme with similar characteristics to BMP2
These formulations are based on a double emulsion technique with solvent evaporation
(wateroilwater WO W) They differ mainly in the phase in which the surfactant (Pluronicreg
F68) is added water (W-F68) or oil (O-F68) This non-ionic polymeric surfactant can modulate
a series of properties of the transporter nanosystem in which it is integrated reducing the size of
the NPs increasing their colloidal stability and facilitating the protection of the encapsulated
biomolecule Furthermore thanks to its superficial arrangement and the hydrophilicity of its polar
12
tails interaction with the mononuclear phagocytic system is reduced with an improvement in
biodistribution by increasing its circulation time after intravenous administration in a living
organism The colloidal properties of these systems have been analyzed using different
experimental techniques (morphology by SEM and STEM hydrodynamic size by DLS and NTA
electrophoretic mobility temporal stability in different media as well as the encapsulation
release pattern and bioactivity of the lysozyme Likewise an interfacial characterization of the
surfactant-protein interaction was carried out in the first water-oil emulsion for each formulation
procedure by analyzing the surface tension and elasticity Finally we analyzed the cellular uptake
by human mesenchymal stromal cells and cytotoxicity for both nanosystems Through the two
formulations O-F68 and W-F68 solid NPs of spherical morphology are obtained although in
one case the system presents monodispersity with diameters around 120 nm (O-F68) while in
the other a Polydisperse nanosystem with particle diameters between 100 and 500 nm (W-F68)
As a more relevant result we observed that the encapsulation efficiency the release and the
bioactivity of lysozyme have been better maintained with the W-F68 formulation method In this
case given the heterogeneity of sizes one could speak of a promising multimodal system to
encapsulate proteins with strong biological activity that allows a dual delivery at the extra- and
intracellular level facilitating protein activity on the cell surface and in the cytoplasm After
developing and optimizing the synthesis method for lysozyme-loaded PLGA NPs we tried to
adapt the formulation to achieve encapsulation of the therapeutic protein BMP-2 Thus based on
the results obtained with lysozyme it was decided to use the W-F68 synthesis procedure to favor
the protection of protein molecules and their biological activity With this formulation spherical
NPs with the aforementioned multimodal size between 100 and 500 nm have been obtained with
good reproducibility which would allow extra- and intracellular delivery In addition to NPs with
encapsulated BMP2 a nanosystem has been obtained in which BMP2 is not encapsulated but is
superficially co-adsorbed with a stabilizing protein such as bovine serum albumin Again a
complete physico-chemical and biological characterization of both NPs systems is carried out
13
analyzing the previously indicated properties that is morphology and size surface charge
colloidal and temporal stability encapsulation and release pattern
It is known that release kinetics in PLGA-based polymer systems are highly dependent on the
hydrolytic degradation of the polymer However the short-time release is influenced by other
physical processes and it is crucial to avoid an excessive initial discharge especially if the
application of this nanotechnology is to be optimized in very important bone regeneration
processes in dentistry Consequently we have focused on the analysis of the release pattern of
BMP2 at short times using different techniques and comparing the behavior of the two NPs
systems with the encapsulated or superficially adsorbed protein Finally the biological activity of
NPs loaded with BMP2 has been analyzed by in vitro studies of cell proliferation migration and
osteogenic differentiation using mesenchymal stromal cells obtained from human alveolar bone
(ABSC) Based on all this it can be confirmed that NPs with encapsulated BMP2 present an
adequate release pattern in the short term maintaining a sustained protein supply and adequate
biological activity for very low initial doses of BMP2
14
LISTA DE PUBLICACIONES
1 Ortega-Oller I Padial-Molina M Galindo-Moreno P OrsquoValle F Joacutedar-Reyes A B
Peula-Garciacutea J M Bone Regeneration from PLGA Micro-Nanoparticles Biomed Res
Int 2015 vol 2015 1ndash18 doi1011552015415289 IF Q2 Rank 82161 JIFpercentil
494 Nordm de citas 33
2 Ortega-Oller I del Castillo-Santaella T Padial-Molina M Galindo-Moreno P Joacutedar-
Reyes A B Peula-Garciacutea J M Dual delivery nanosystem for biomolecules
Formulation characterization and in vitro release Colloids Surfaces B Biointerfaces
2017 159 586ndash595doi 101016jcolsurfb201708027 IF Q1 Rank1272 JIF
percentil 826 Nordmcitas 5
3 del Castillo-Santaella T Ortega-Oller I Padial-Molina M OrsquoValle F Galindo-
Moreno P Joacutedar-Reyes A B Peula-Garciacutea J M Formulation Colloidal
Characterization and In Vitro Biological Effect of BMP-2 Loaded PLGA Nanoparticles
for Bone Regeneration Pharmaceutics 2019 11(8) 388
doi103390pharmaceutics11080388 IF Q1 Rank26267 JIFpercentil 905 Nordmcitas
3
15
16
Iacutendice
0 GLOSARIO (lista de abreviaturas) helliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphelliphellip 19
1 INTRODUCCIOacuteN 23
11 BMPS ACCIOacuteN Y REGULACIOacuteN 24
111 Uso cliacutenico de la BMP-2 27
12 PARTIacuteCULAS COLOIDALES POLIMEacuteRICAS PARA ENCAPSULAR MOLEacuteCULAS HIDROFIacuteLICAS 30
121Meacutetodos de siacutentesis 33
123 Tamantildeo y morfologiacutea de las partiacuteculas 35
13AGENTES ESTABILIZADORES 39
131 Estabilidad coloidal 39
132 Eficacia de encapsulacioacuten y bioactividad 42
14 PATROacuteN DE LIBERACIOacuteN 44
15 VECTORIZACIOacuteN ENTREGA DIRIGIDA 52
16 INGENIERIacuteA TISULAR SOPORTES 3D O ldquoSCAFFOLDSrdquo 53
2 HIPOacuteTESIS 55
3 OBJETIVOS 56
31 OBJETIVO PRINCIPAL 56
32 OBJETIVOS SECUNDARIOS 56
4 NANOSISTEMA DE ENTREGA DOBLE PARA BIOMOLEacuteCULAS FORMULACIOacuteN CARACTERIZACIOacuteN
Y LIBERACIOacuteN IN VITRO 58
41 ANTECEDENTES 58
42 MATERIALES Y MEacuteTODOS 60
421Formulacioacuten de las nanoparticulas 60
422 Limpieza y almacenamiento 61
423 Caracterizacioacuten de las nanoparticulas 62
424 Estabilidad coloidal y temporal en biologiacutea media 63
425 Actividad bioloacutegica e interacciones 64
43 RESULTADOS Y DISCUSIOacuteN 65
17
431 Formulacioacuten de las nanoparticulas 65
432 Caracterizacioacuten de las Nanopartiacuteculas 69
433 Actividad bioloacutegica e interacciones 84
5 FORMULACIOacuteN CARACTERIZACIOacuteN COLOIDAL Y EFECTO BIOLOacuteGICO IN VITRO DE
NANOPARTIacuteCULAS DE PLGA CARGADAS CON BMP-2 PARA LA REGENERACIOacuteN OacuteSEA 96
51 ANTECEDENTES 96
52 MATERIALES Y MEacuteTODOS 98
521Siacutentesis de nanoparticulas 98
522 Caracterizacioacuten de nanopartiacuteculas morfologiacutea tamantildeo concentracioacuten y movilidad electrocineacutetica
101
523 Estabilidad coloidal y temporal en medios bioloacutegicos 101
524 Interacciones celulares 102
53 RESULTADOS Y DISCUSIOacuteN 105
531Formulacioacuten de nanoparticulas 105
532Caracterizacioacuten de nanopartiacuteculas 109
533Actividad bioloacutegica e interacciones 118
6 CONCLUSIONES 125
7 CONFLICTO DE INTERESES 127
8 RECURSOS ECONOacuteMICOS 127
9 BIBLIOGRAFIacuteA 128
10 ANEXO MATERIAL SUPLEMENTARIO 154
- Enlace a videos
11 ANEXO DE PUBLICACIONES 156
-Artiacuteculo 1 Bone regeneration from PLGA Micro-Nanoparticles
-Artiacuteculo 2 Dual delivery nanosystem for biomolecules Formulation characterization and
in vitro release
18
-Artiacuteculo 3 Formulation Colloidal Characterization and In Vitro Biological Effect of
BMP-2 Loaded PLGA Nanoparticles for Bone Regeneration
12 ANEXO DE ORIGINALIDAD 158
19
LISTA DE ABREVIACIONES
NP Nanopartiacuteculas
MP Micropartiacuteculas
MSC Ceacutelulas mesenquimales
BMP Proteiacutena morfogeneacutetica oacutesea
Rh BMP Proteiacutena morfogeneacutetica oacutesea recombinante
BMP I y II Proteiacutena morfogeneacutetica oacutesea receptora I y II
GF Factor de crecimiento
PDGF Factor de crecimiento derivado de plaquetas
FGF Factor de crecimiento de fibroblastos
IGF Factor de crecimiento de insulina
TGF- Factor de crecimiento transformante
RUNX2 Factor de transcripcioacuten
MPS Sistema fagociacutetico mononuclear
hMSC Ceacutelulas mesenquimales humanas
ABSC Ceacutelulas estromales mesenquimales oacutesea
OSX Osterix
LMP Proteiacutena de mineralizacioacuten de dominio Lim
SEM Microscopia electroacutenica de barrido
STEM Microscopia electroacutenica de transmisioacuten de barrido
PLA Aacutecido poli-lactico
PLGA Aacutecido poli-lactico co-glicolico
LYS Lisozima
LYSF Lisozima final (encapsulada)
20
F68 Pluronic (W-F68= en agua) (O-F68= en aceite)
WOW Doble emulsioacuten de agua en aceite
AP Fase acuosa
OP Fase orgaacutenica
SA Albuacutemina seacuterica
BSA Albuacutemina seacuterica bovina
HSA Albuacutemina seacuterica humana
PVA Alcohol poliviniacutelico
PBS Tampoacuten fosfato salino
PB Tampoacuten fosfato
FBS Suero fetal bovino
PEO Oacutexido de polietileno
DCM Diclorometano
EA Acetato de etilo
FITC Isotiocianato de fluoresceiacutena
DPPC Dipalmitoil-fosfatidilcolina
PGE Polietilenglicol
DC Aacutecido dexosicoacutelico
BCA Aacutecido bicinconiacutenico
DTT Ditiotreitol
SDS Duodecil sulfato de sodio
ALP Fosfatasa alcalina
GAPDH Gliceraldehiacutedo-3-fosfato deshidrogenasa
SDS Gel duodecilsulfato de sodio
PI3K Fosfoinositida 3-quinasa
ALP Fosfatasa alcalina
21
SDS-PAGE Electroforesis en gel poliacrilamida con duodecilsulfato soacutedico
PDI Iacutendice de polidispersidad
DLS Dispersioacuten de luz dinaacutemica
EE Eficacia de encapsulacioacuten
SRB Absorbancia de sulforamida
DL Carga del faacutermaco
FDA Administracioacuten de medicamentos y alimentos
RMN Resonancia magneacutetica nuclear
NTA Anaacutelisis de seguimiento de nanopartiacuteculas
NLS Sentildeales de localizacioacuten nuclear
DMEM Medio Eagle modificado con dulbecco
23
1 INTRODUCCIOacuteN
La regeneracioacuten oacutesea es uno de los principales desafiacuteos a los que nos enfrentamos en la cliacutenica
diariamente Inmediatamente despueacutes de la extraccioacuten de un diente los procesos bioloacutegicos
normales remodelan el hueso alveolar limitando en algunos casos la posibilidad de una futura
colocacioacuten de implante En los uacuteltimos antildeos han sido estudiadas diferentes estrategias para llevar
a cabo la preservacioacuten de ese hueso Otras afecciones como el traumatismo la cirugiacutea de
reseccioacuten tumoral o las deformidades congeacutenitas requieren requisitos teacutecnicos y bioloacutegicos auacuten
mayores para generar la estructura oacutesea necesaria para la rehabilitacioacuten oclusal del paciente Para
superar estas limitaciones anatoacutemicas en teacuterminos de volumen oacuteseo existen diferentes enfoques
para mejorar la osteointegracioacuten del implante o para aumentar la anatomiacutea del hueso donde se
colocaraacute el futuro implante (M Padial-Molina P Galindo-Moreno 2009) (Al-Nawas and
Schiegnitz 2014) El injerto oacuteseo autoacutegeno todaviacutea se considera el ldquogold estaacutendarrdquo debido a sus
propiedades osteogeacutenicas osteoconductivas y osteoinductivas (Katranji Fotek and Wang 2008)
(Misch 1987) Sin embargo tambieacuten presenta varias limitaciones incluida la necesidad de una
segunda cirugiacutea disponibilidad limitada y morbilidad en el aacuterea donante (Myeroff and
Archdeacon 2011) Por lo tanto otros biomateriales como injertos alogeacutenicos e injertos
xenogeacutenicos con osteoconductividad y capacidades osteoinductivas (Avila et al 2010) (Froum
et al 2006) fueron propuestos (Galindo-Moreno et al 2007) (Galindo-Moreno et al 2011) asiacute
como biomateriales aloplaacutesticos (Wheeler 1997) con potencial osteoconductivo Todos estos
materiales aunque aceptables no son adecuados en muchas condiciones y generalmente requieren
una consideracioacuten adicional en el proceso de decisioacuten (Wallace and Froum 2003) Ademaacutes la
cantidad y calidad de hueso que se puede obtener con estos materiales a menudo es limitada
El uso de moleacuteculas bioactivas por siacute solas o en combinacioacuten con los materiales descritos
previamente se ha convertido por lo tanto en un aacuterea de intereacutes principal gracias a su alto
potencial Al usar este tipo de procedimientos es importante considerar 1) el meacutetodo de
administracioacuten y 2) la moleacutecula por siacute misma Las moleacuteculas bioactivas pueden transportarse al
24
aacuterea del defecto como una solucioacuten o un gel incrustados en esponjas adheridos a scaffolds soacutelidos
y maacutes recientemente incluidos en partiacuteculas de diferentes tamantildeos Usando estos meacutetodos se
puede acudir a una gran diversidad de biomoleacuteculas como PDGF (factor de crecimiento
derivado de plaquetas) FGF (factor de crecimiento de fibroblastos) IGF (factor de crecimiento
de insulina) RUNX2 osterix (Osx) proteiacutena de mineralizacioacuten de dominio LIM (LMP) BMP
(proteiacutena morfogeacutenica oacutesea) y maacutes recientemente periostin como candidatos potenciales para los
procedimientos de regeneracioacuten dentro de la cavidad oral incluidos los tejidos oacuteseos y
periodontales (Padial-Molina and Rios 2014) (Padial-Molina Volk and Rios 2014) Estas
moleacuteculas se probaron solas o en combinacioacuten con ceacutelulas madre (Behnia et al 2012) utilizando
varias estrategias in vitro e in vivo (Padial-Molina et al 2012)
11 BMPs Accioacuten y regulacioacuten
En regeneracioacuten oacutesea y en particular los factores de crecimiento morfogeneacuteticos oacuteseos (BMP)
son probablemente el grupo de moleacuteculas maacutes comuacuten Desde 1965 cuando Urist (Urist 1965)
demostroacute que las BMPs oacuteseas extraiacutedas podriacutean inducir la formacioacuten de hueso y cartiacutelago cuando
se implantan en tejido animal un alto nuacutemero de artiacuteculos han probado su aplicacioacuten in vivo y su
base bioloacutegica cuando se usan en defectos oacuteseos (Boyne and Jones 2004) (Wang et al 1990)
(Wozney 1992) Las BMPs son miembros de la suacuteper familia de proteiacutenas TGF-β (Barboza
Caula and Machado 1999) La familia de proteiacutenas BMP agrupa maacutes de 20 proteiacutenas
morfogeneacuteticas homodimeacutericas o heterodimeacutericas que funcionan en muchos tipos y tejidos
celulares no todos tienen que ser necesariamente osteogeacutenicos (Ana Claudia Carreira et al
2014) Las BMPs se pueden dividir en 4 subfamilias seguacuten su funcioacuten y secuencia siendo BMP-
2 -4 y -7 las que tienen un fuerte potencial osteogeacutenico (Ana Claudia Carreira et al 2014) Las
acciones de las BMPs incluyen la condrogeacutenesis la osteogeacutenesis la angiogeacutenesis y la siacutentesis de
la matriz extracelular (Bustos-Valenzuela et al 2011) Dentro de esta familia de proteiacutenas BMP-
2 ha sido la maacutes estudiada Tiene propiedades osteoinductoras que promueven la formacioacuten de
25
nuevo hueso al iniciar estimular y amplificar la cascada de la formacioacuten oacutesea a traveacutes de la
quimiotaxis y la estimulacioacuten de la proliferacioacuten y diferenciacioacuten del linaje celular osteoblaacutestico
(Myeroff and Archdeacon 2011) (Boyne and Jones 2004) (Wozney 1992) (Barboza Caula and
Machado 1999) La ausencia de eacutesta como se estudioacute en los modelos eliminatorios conduce a
fracturas espontaacuteneas que no cicatrizan con el tiempo (Tsuji et al 2006) De hecho otros modelos
han demostrado que la ausencia de cualquiera de estas dos BMP-4 (Tsuji et al 2008) o -7 (Tsuji
et al 2010) no conducen a la formacioacuten de hueso y deterioro como demuestra el efecto producido
por BMP-2 sola (Chen Deng and Li 2012)
Muchos tipos de ceacutelulas en el tejido oacuteseo producen BMP como las ceacutelulas osteoprogenitoras
osteoblastos condrocitos plaquetas y ceacutelulas endoteliales Esta BMP secretada se almacena en la
matriz extracelular donde interactuacutea principalmente con el colaacutegeno tipo IV (Ramel and Hill
2012) Durante los procesos de reparacioacuten y remodelacioacuten la actividad absorbente de los
osteoclastos induce la liberacioacuten de BMP al medio para que se suspenda la funcioacuten de absorcioacuten
y eacutesta pueda interactuar con las ceacutelulas cercanas para iniciar el consecuente proceso osteogeacutenico
(A C Carreira et al 2014)
La BMP en la matriz extracelular se une a los receptores de la superficie celular BMPR-I y II
y activa las proteiacutenas citoplasmaacuteticas Smad o la viacutea MAPK (Deschaseaux Sensebe and Heymann
2009) Cuando BMPR-I se activa BMPR-II se engancha y se activa tambieacuten (Mueller and Nickel
2012) La activacioacuten del complejo BMPR-I y BMPR-II conduce a la activacioacuten de varios Smads
(1 5 y 8) que tambieacuten activan Smad-4 y todos forman complejos proteicos que se transportan al
nuacutecleo donde Runx2 Dlx5 y los genes Osterix (importantes en la osteogeacutenesis) se activan (Chen
Deng and Li 2012) (Ramel and Hill 2012) (Figura 1) De forma similar cuando se activa la ruta
de MAPK conduce a la induccioacuten de la transcripcioacuten de Runx2 y por lo tanto a la diferenciacioacuten
oacutesea (Sieber et al 2009) Tambieacuten se han descrito varios antagonistas extracelulares e
intracelulares que incluyen noggin chordin y gremlin o Smad-6 -7 y -8b respectivamente
(Sapkota et al 2007)
26
Figura 1 Representacioacuten esquemaacutetica de la ruta molecular principal de BMP a la
osteogeacutenesis Las BMP interactuacutean con los receptores de la superficie celular I y II para activar
Smads 1 5 y 8 Estos Smads activados activan Smad 4 Todos juntos como un complejo de
proteiacutenas activan Runx2 Dlx5 y Osterix
Foto tomada de Articulo Ortega-Oller I Padial-Molina M Galindo-Moreno P OacuteValle F
Jodar-Reyes AB Peula-Garcia JM Bone regeneration form Plga Micro-Nanoparticles BioMed
Research International 2015 415289 (2015)
27
111Uso cliacutenico de la BMP-2
Hoy en diacutea la BMP-2 estaacute disponible comercialmente bajo diferentes nombres de marcas y
concentraciones Por lo general consiste en una esponja absorbible de colaacutegeno fijada con BMP-
2 humana recombinante En 2002 fue aprobado por la FDA como una alternativa de injerto oacuteseo
autoacutegeno en la fusioacuten intersomaacutetica lumbar anterior (McKay Peckham and Badura 2007) Maacutes
tarde en 2007 la FDA aproboacute el uso de rhBMP-2 como una alternativa para el injerto oacuteseo
autoacutegeno en el aumento de los defectos de la cresta alveolar asociados con la extraccioacuten del diente
en la neumatizacioacuten del seno maxilar (McKay Peckham and Badura 2007)
Ademaacutes de las aplicaciones en estudios cliacutenicos de columna donde se usan concentraciones
muy altas (AMPLIFYTM rhBMP-2 40 mg) los estudios cliacutenicos han apoyado su uso en la
cavidad oral Las BMP se han utilizado en la regeneracioacuten periodontal la terapeacuteutica oacutesea la
osteointegracioacuten de implantes la cirugiacutea oral con fines ortodoacutencicos la reparacioacuten de secuelas
derivadas de la patologiacutea oacutesea la osteogeacutenesis por distraccioacuten y la cirugiacutea reparadora de
endodoncia (A C Carreira et al 2014) (Hong et al 2013) Sin embargo han mostrado resultados
maacutes prometedores en casos en los que solo se regeneraraacute el tejido oacuteseo incluido el desarrollo del
sitio pre-implantario la elevacioacuten de seno el aumento de cresta vertical y horizontal y la
cicatrizacioacuten de cirugiacuteas de implantes dentales (Spagnoli and Marx 2011) En este sentido se
evidencioacute que el uso de rhBMP-2 indujo la formacioacuten de hueso adecuado para la colocacioacuten de
implantes dentales y su osteointegracioacuten (Nevins et al 1996) Ademaacutes parece que el hueso recieacuten
formado tiene propiedades similares al hueso nativo y por lo tanto es capaz de soportar las
fuerzas oclusales que ejerce la dentadura durante su funcioacuten masticatoria (Boyne et al 2005)
En resumen los estudios nombrados concluyeron que rhBMP-2 induce la formacioacuten de nuevo
hueso con una calidad y cantidad comparable al inducido por la cicatrizacioacuten del propio paciente
e incluso en algunos de los casos se informoacute de haber obtenido una cantidad y calidad de hueso
mayor a la que se hubiese obtenido por la viacutea de cicatrizacioacuten normal del paciente (Lee et al
2013)
28
Por el contrario estudios recientes revelan graves complicaciones despueacutes de su uso (Ronga et
al 2013) Ademaacutes se han asociado efectos carcinogeacutenicos a altas dosis lo que llevoacute a los autores
a enfatizar en la necesidad de mejores pautas en el uso cliacutenico de BMP (Devine et al 2012) No
tan draacutesticos son los uacuteltimos estudios que destacan los efectos secundarios negativos y los riesgos
de su aplicacioacuten haciendo gran hincapieacute en el sesgo potencial de la investigacioacuten patrocinada por
la industria no reproducible especialmente cuando se utiliza en la meacutedula espinal (Fu et al 2013)
(Carragee Hurwitz and Weiner 2011) (Simmonds et al 2013) Se observoacute tambieacuten que el uso
de rhBMP-2 aumenta el riesgo de complicaciones en la zona tratada disfagia con alta eficacia y
dantildea la tergiversacioacuten mediante informes selectivos publicaciones duplicadas y subregistros (Fu
et al 2013) Especiacuteficamente en el campo de la regeneracioacuten oacutesea dentro de la cavidad oral un
estudio de elevacioacuten de seno concluyoacute que el uso de BMP-2 promueve efectos negativos en la
formacioacuten oacutesea cuando se combina con matriz oacutesea bovina inorgaacutenica vs hueso bovino
inorgaacutenico solo (Kao et al 2012) en contraste con artiacuteculos y revisiones previas (Torrecillas-
Martinez et al 2013) Al tomar en cuenta esta informacioacuten se puede concluir que es de extrema
importancia tener cuidado con el uso cliacutenico de nuevos productos evitando las aplicaciones no
clasificadas Tambieacuten es importante resaltar la necesidad de maacutes y mejores investigaciones
cliacutenicas
Para superar estas limitaciones el uso de ceacutelulas mesenquimales especificas (MSC) autoacutelogas
modificadas por BMP-2 ex vivo (Chung et al 2012) en los uacuteltimos antildeos estaacute dando lugar a
explorar nuevas estrategias como la encapsulacioacuten de la proteiacutena en diferentes biomateriales o el
suministro mediante terapia geacutenica
El desarrollo de estas tecnologiacuteas se basa en algunos hechos bioloacutegicos Los efectos in vitro de
las BMP se observan en dosis muy bajas (5-20 ngml) aunque las rhBMP actuales disponibles
comercialmente se usan en dosis grandes (hasta 40 mg de algunos productos) (A C Carreira et al
2014) Esto probablemente se deba a un consumo proteoliacutetico intenso durante las primeras fases
posquiruacutergicas Es importante conocer la secuencia adecuada de los procesos bioloacutegicos que
29
conducen a la cicatrizacioacuten normal del tejido Por lo tanto este conocimiento se puede usar para
intervenir en el marco temporal especiacutefico en el que se pretende que actuacutee nuestra terapia (Padial-
Molina et al 2012) Tambieacuten es importante tener en cuenta que el papel de otras viacuteas moleculares
y la diafoniacutea entre los diferentes componentes que llevan a cabo la regeneracioacuten oacutesea todaviacutea no
se entiende perfectamente y por lo tanto se debe realizar maacutes investigacioacuten
Lo que hasta ahora se sabe en resumen es que las BMP y especiacuteficamente BMP-2 son uacutetiles
para promover la regeneracioacuten oacutesea (A C Carreira et al 2014) Sin embargo las rutas disponibles
de administracioacuten local basadas en la activacioacuten de las BMP entregadas por esponjas de colaacutegeno
presentan importantes limitaciones (Chung et al 2012) En primer lugar la proteiacutena se inactiva
raacutepidamente Por lo tanto su accioacuten bioloacutegica desaparece puede ser incluso antes de que se forme
el coaacutegulo de sangre el cual se forma despueacutes de la cirugiacutea Ademaacutes la distribucioacuten de la BMP
en una suspensioacuten liacutequida incrustada en una esponja de colaacutegeno hace que sea imposible estar
seguro de que la proteiacutena estaacute alcanzando el objetivo ideal Debido a eso deben desarrollarse
nuevas formas de administracioacuten de BMP-2 Estas nuevas tecnologiacuteas tienen que garantizar una
mayor vida media de la proteiacutena y una liberacioacuten escalonada para aumentar los efectos sobre los
objetivos celulares deseados La biotecnologiacutea abre la puerta para poder proporcionar una
solucioacuten a estas limitaciones
De esta manera las nanopartiacuteculas biodegradables (nanoesferas y nanocaacutepsulas) fueron
desarrolladas como una herramienta importante y prometedora para la administracioacuten de
macromoleacuteculas a traveacutes de aplicaciones parenterales mucosas y toacutepicas (Barratt 2003)
(Bramwell and Perrie 2005) (Csaba Garcia-Fuentes and Alonso 2006) (M J Santander-Ortega
et al 2010) Los poliacutemeros biodegradables bien establecidos tales como poli (aacutecido D L-laacutectico)
o poli (D L-laacutectico-co-glicoacutelico) se estaacuten utilizando ampliamente en la preparacioacuten de
nanopartiacuteculas en las uacuteltimas deacutecadas debido a su biocompatibilidad y biodegradabilidad
completa (Jiang et al 2005) Sin embargo se sabe que ciertas macromoleacuteculas como proteiacutenas
o peacuteptidos pueden perder actividad durante su encapsulacioacuten almacenamiento administracioacuten y
30
liberacioacuten (Kumar Soppimath and Nachaegari 2006) Para superar este problema la adicioacuten de
estabilizadores tales como oacutexido de polietileno (PEO) o la co-encapsulacioacuten con otras
macromoleacuteculas y sus derivados parece ser una estrategia prometedora
12 Partiacuteculas coloidales polimeacutericas para encapsular moleacuteculas hidrofiacutelicas
Generalmente las partiacuteculas coloidales polimeacutericas son sistemas consistentes con una forma
esfeacuterica homogeacutenea compuesta por poliacutemeros naturales o sinteacuteticos Con el fin de encapsular
moleacuteculas hidroacutefilas como proteiacutenas o aacutecidos nucleicos para ello es necesario optimizar la
composicioacuten polimeacuterica y el meacutetodo de siacutentesis En este proceso se debe lograr una alta eficacia
de encapsulacioacuten el mantenimiento de la actividad bioloacutegica de la biomoleacutecula encapsulada y la
obtencioacuten de un patroacuten de liberacioacuten adecuado (Danhier et al 2012) (Kumari Yadav and Yadav
2010) (Makadia and Siegel 2011) Varios sistemas de administracioacuten de BMP2 (y otros GF) que
usan partiacuteculas polimeacutericas estaacuten descritos en la bibliografiacutea La mayoriacutea de ellos son sistemas
microparticulados Casi en su totalidad todos ellos usan el copoliacutemero de PLGA biocompatible
y biodegradable como componente principal (Mohamed and van der Walle 2008) (Silva et al
2007)
Teniendo en cuenta la incorporacioacuten de BMP2 al sistema portador la encapsulacioacuten es
preferible a la adsorcioacuten porque los factores de crecimiento estaacuten maacutes protegidos contra factores
ambientales en el medio y pueden tener un mejor control sobre la administracioacuten y liberacioacuten para
alcanzar las concentraciones deseadas en sitio y tiempo especiacuteficos (Zhang and Uludag 2009)
Normalmente si los GF estaacuten relacionados con los procesos de regeneracioacuten oacutesea las nano-
micropartiacuteculas quedan atrapadas en un segundo sistema como hidrogeles o scaffolds de
ingenieriacutea tisular que tambieacuten juegan un papel importante en el perfil de liberacioacuten de los GF de
estas partiacuteculas (Zhang and Uludag 2009) Las nano-micropartiacuteculas han permitido el desarrollo
de scaffolds multiescala lo que facilita el control de la arquitectura interna y los patrones
31
adecuados de los gradientes mecaacutenicos de las ceacutelulas asiacute como los factores de sentildealizacioacuten (Santo
et al 2012)
Todos los pasos desde el meacutetodo de siacutentesis y sus caracteriacutesticas el proceso de encapsulacioacuten
o la modificacioacuten final de la superficie para una entrega dirigida determinan las caracteriacutesticas de
estos sistemas y su objetivo principal la liberacioacuten controlada de GF bioactivos
Figura 2 Procedimiento de doble emulsioacuten (emulsioacuten agua aceite agua W1OW2) para
obtener micropartiacuteculas nanopartiacuteculas de PLGA
En la figura 2 se muestra el esquema de siacutentesis de micro-nanopartiacuteculas de PLGA mediante
un procedimiento de doble emulsioacuten Dependiendo de las condiciones de siacutentesis (estabilizadores
disolventes y procedimiento de mezcla) es posible obtener micro-nanoesferas con una matriz
uniforme o micro-nanocaacutepsulas con una estructura corteza-nuacutecleo Las inmunopartiacuteculas
32
utilizadas para la administracioacuten dirigida pueden obtenerse uniendo moleacuteculas especiacuteficas de
anticuerpos en la superficie de la partiacutecula
33
121Meacutetodos de siacutentesis
Es posible encontrar varios procedimientos para encapsular moleacuteculas hidrofiacutelicas como
proteiacutenas o aacutecidos nucleicos en nano micropartiacuteculas polimeacutericas Se ha observado que las
teacutecnicas de separacioacuten de fases (Tran Swed and Boury 2012) o secado por pulverizacioacuten (Ertl et
al 2000) encapsulan moleacuteculas hidroacutefilas Sin embargo en el caso de las proteiacutenas el
procedimiento maacutes utilizado para encapsularlas en micropartiacuteculas y nanopartiacuteculas de PLGA es
la teacutecnica de evaporacioacuten con disolvente de doble emulsioacuten (WOW) (Makadia and Siegel 2011)
(Hans and Lowman 2002) En la figura 2 se presenta una descripcioacuten esquemaacutetica de esta teacutecnica
De manera general el PLGA se disuelve en un disolvente orgaacutenico y se emulsiona usando
agitacioacuten mecaacutenica o sonicacioacuten con agua que contiene una cantidad apropiada de proteiacutena
Por lo tanto se obtiene una emulsioacuten W1O primaria En la segunda fase esta emulsioacuten se
vierte en una gran fase polar que conduce a una precipitacioacuten inmediata de las partiacuteculas como
consecuencia de la contraccioacuten del poliacutemero alrededor de las gotitas de la emulsioacuten primaria Esta
fase puede estar compuesta por una solucioacuten acuosa de un estabilizador (surfactante) o mezclas
de etanol y agua (Blanco and Alonso 1998) (Csaba et al 2005) Despueacutes de agitar el resultado
del disolvente orgaacutenico se extrae raacutepidamente por evaporacioacuten al vaciacuteo En este procedimiento se
ha probado una amplia lista de diferentes modificaciones con el fin de obtener un sistema micro
nanoportador con estabilidad coloidal adecuada alta eficacia de encapsulacioacuten bioactividad
adecuada y finalmente un perfil de liberacioacuten a largo plazo con una miacutenima descarga inicial
El objetivo es evitar que se libere una gran cantidad de proteiacutena (gt 60) muy raacutepidamente (24
horas) que es uno de los mayores problemas de un sistema de liberacioacuten controlada (Mohamed
and van der Walle 2008)
34
122 Disolvente orgaacutenico
Hans y colaboradores muestran diferentes ejemplos de solventes orgaacutenicos usados en muacuteltiples
procesos de emulsioacuten Normalmente pueden usarse diclorometano (DMC) acetato de etilo
acetona y otras mezclas (Hans and Lowman 2002) En el primer paso seriacutea una buena eleccioacuten
escoger un buen solvente orgaacutenico con baja solubilidad en agua para facilitar el proceso de
emulsioacuten y bajo punto de ebullicioacuten para una faacutecil evaporacioacuten Sin embargo la estructura de las
moleacuteculas de proteiacutenas encapsuladas puede verse afectada y los procesos de desnaturalizacioacuten y
peacuterdida de actividad bioloacutegica aparecen cuando interactuacutean con un solvente orgaacutenico tiacutepico como
DMC (Danhier et al 2012) El acetato de etilo por otro lado ejerce efectos menos
desnaturalizantes con una menor incidencia en la bioactividad de las proteiacutenas encapsuladas
(Sturesson and Carlfors 2000)
Otros factores importantes relacionados con el disolvente orgaacutenico son sus propiedades fiacutesicas
que afectan la forma en que las moleacuteculas del poliacutemero se auto organizan en la envoltura de las
gotas de la emulsioacuten y modifican la morfologiacutea de las nanopartiacuteculas y la eficacia de
encapsulacioacuten (Rosca Watari and Uo 2004) De esta forma una mayor solubilidad en agua del
disolvente orgaacutenico es decir acetato de etilo favorece una eliminacioacuten maacutes raacutepida del disolvente
Ademaacutes la velocidad de eliminacioacuten del disolvente puede controlarse ajustando el volumen de la
fase polar asiacute como la tensioacuten de cizallamiento durante la segunda etapa de la emulsioacuten Un
aumento de estos dos paraacutemetros aumenta la velocidad de difusioacuten del acetato de etilo desde las
micropartiacuteculas primarias a la fase acuosa externa lo que da como resultado su raacutepida
solidificacioacuten (Meng et al 2003) Tambieacuten mejora la eficiencia de encapsulacioacuten y minimiza el
tiempo de contacto entre las moleacuteculas de proteiacutena y el solvente orgaacutenico (Ghaderi and Carlfors
1997) obteniendo al mismo tiempo un menor efecto de raacutefaga y una liberacioacuten maacutes lenta del
faacutermaco desde las micropartiacuteculas (Meng et al 2003)
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123 Tamantildeo y morfologiacutea de las partiacuteculas
El tamantildeo de la partiacutecula es un paraacutemetro importante y uno de los objetivos principales del
sistema de liberacioacuten polimeacuterica Las microesferas desde unos pocos microacutemetros hasta 100 μm
son adecuadas para el suministro oral la adhesioacuten a la mucosa o el uso interior del armazoacuten es
decir para la regeneracioacuten oacutesea La dimensioacuten a nano-escala del soporte ofrece una versatilidad
mejorada cuando se compara con partiacuteculas de mayor tamantildeo Esto se debe a que tienen una
mayor estabilidad coloidal una mejor dispersabilidad y biodisponibilidad una superficie maacutes
reactiva y ademaacutes pueden administrar proteiacutenas o faacutermacos dentro y fuera de las ceacutelulas
correspondientes (Wang et al 2012) BMP2 promueve la formacioacuten de hueso e induce la
expresioacuten de otras BMP e inicia la viacutea de sentildealizacioacuten desde la superficie de la ceacutelula unieacutendose
a dos receptores de superficie diferentes (Bustos-Valenzuela et al 2011) Por lo tanto las
partiacuteculas portadoras de BMP2 deben liberarlo en el medio extracelular Dado que la ingesta
celular de nanopartiacuteculas de PLGA es muy raacutepida el proceso de incorporacioacuten puede verse
limitado por un aumento en el tamantildeo de nano a micropartiacuteculas (Xiong et al 2011) Sin
embargo la interaccioacuten entre partiacuteculas y ceacutelulas estaacute fuertemente influenciada por el tamantildeo de
la partiacutecula Si se desea la internalizacioacuten de la ceacutelula la partiacutecula debe estar comprendida en la
escala submicroacutemica en un intervalo entre 2-500 nm (Chou Ming and Chan 2011) Ademaacutes este
tamantildeo es necesario para una distribucioacuten raacutepida despueacutes de la administracioacuten parenteral con el
fin de alcanzar diferentes tejidos a traveacutes de diferentes barreras bioloacutegicas Ademaacutes la ingesta de
macroacutefagos se minimiza con un diaacutemetro de nanopartiacuteculas por debajo de 200 nm e incluso maacutes
pequentildeo (Hans and Lowman 2002) (Manuel J Santander-Ortega Lozano-Loacutepez et al 2010)
Como se discutioacute en un artiacuteculo escrito por Yang y colaboradores (Yang Chung and Ng 2001)
ligeras modificaciones del procedimiento de siacutentesis pueden suponer efectos draacutesticos en el
tamantildeo o la morfologiacutea de las partiacuteculas y por lo tanto en la eficacia de encapsulacioacuten de
proteiacutenas y la liberacioacuten cineacutetica
36
Figura 3 Fotografiacutea mediante microscopiacutea electroacutenica de barrido (SEM) de nanopartiacuteculas
de PLGA obtenidas mediante un procedimiento de emulsificacioacuten de doble emulsioacuten Es un
sistema con forma esfeacuterica baja polidispersidad y una escala nanoscoacutepica que muestra las
propiedades deseadas para una distribucioacuten fisioloacutegica adecuada y la internalizacioacuten celular
En los procesos de doble emulsioacuten la primera etapa de emulsioacuten determina en gran medida el
tamantildeo de la partiacutecula mientras que la segunda etapa de emulsioacuten caracterizada por la
eliminacioacuten del disolvente y la precipitacioacuten del poliacutemero afecta principalmente a la morfologiacutea
de la partiacutecula (Rosca Watari and Uo 2004) Sin embargo en este paso el uso de soluciones
surfactantes como el medio polar del segundo proceso de emulsioacuten y la relacioacuten de volumen entre
las fases orgaacutenicas y polares han mostrado una influencia importante en el tamantildeo final (Feczkoacute
Toacuteth and Gyenis 2008)Por lo tanto la eleccioacuten correcta del solvente orgaacutenico la concentracioacuten
del poliacutemero la adicioacuten de surfactante y la energiacutea del proceso de emulsioacuten permiten controlar el
tamantildeo del sistema
37
La incorporacioacuten de poloxaacutemeros (F68) en el disolvente orgaacutenico de la emulsioacuten primaria
ayuda a aumentar la estabilidad coloidal de la primera dispersioacuten al colocarla en el interfaz WO
Esto reduce el tamantildeo de partiacutecula en comparacioacuten con las nanopartiacuteculas de PLGA puro en las
que la uacutenica fuente de estabilidad proviene de la carga eleacutectrica de los grupos carboxilo del PLGA
(Santander-Ortega Bastos-Gonzalez and Ortega-Vinuesa 2007) Es normal obtener micro
nanoesferas ciliacutendricas con un nuacutecleo poroso polimeacuterico En la figura 3 se muestra una
micrografiacutea SEM tiacutepica de nanopartiacuteculas de PLGA obtenidas mediante emulsioacuten WOW usando
una mezcla de disolventes orgaacutenicos (DCM acetona) y etanol agua como segundo medio polar
en la que la forma esfeacuterica y la distribucioacuten uniforme del tamantildeo son las principales
caracteriacutesticas La cubierta exterior polimeacuterica en la segunda etapa de emulsioacuten empujoacute las gotas
de agua hacia el nuacutecleo interno de acuerdo con el proceso de solidificacioacuten (Yang Chia and
Chung 2000) Este proceso permite producir partiacutecula como son estas caacutepsulas con una
estructura nuacutecleo-capa en la que el nuacutecleo interno tiene una baja densidad de poliacutemero
La figura 4 muestra una estructura nuacutecleo-capa tiacutepica en la que el poliacutemero precipita y se
contrae alrededor de las gotas de agua durante el cambio de disolvente de la segunda fase y el
posterior proceso de evaporacioacuten del disolvente orgaacutenico (Fang et al 2014) En este caso el
proceso de solidificacioacuten del poliacutemero se ve influenciado y determinado por la miscibilidad del
disolvente orgaacutenico con la segunda fase polar y la velocidad de eliminacioacuten
38
Figura 4 Nanopartiacuteculas de mezcla PLGA poloxamers188 (a) Fotografiacutea de microscopiacutea
electroacutenica de transmisioacuten (STEM) (b) Fotografiacutea de microscopiacutea electroacutenica de barrido (SEM)
La teacutecnica STEM permite el anaacutelisis de la estructura de nanopartiacuteculas con una regioacuten interna
con baja densidad de poliacutemero que es representativa de nanocaacutepsulas con estructura nuacutecleo-
caparazoacuten
La cubierta polimeacuterica a menudo presenta canales o poros como consecuencia de la extrusioacuten
de agua interna debido a las fuerzas osmoacuteticas Esto puede reducir la eficacia de la encapsulacioacuten
y favorecer una raacutepida fuga inicial con la liberacioacuten en raacutefaga no deseada (Yang Chung and
Ng 2001) Esta modificacioacuten de la estructura interna de las partiacuteculas generalmente se indica
asignando el teacutermino nanoesfera al sistema con un nuacutecleo que consiste en una matriz polimeacuterica
homogeacutenea El agente bioactivo se dispersa dentro de ellas mientras que la estructura nuacutecleo-
capa seriacutea similar a una nanocaacutepsula donde la biomoleacutecula estaacute preferiblemente en la cavidad
acuosa rodeada por la cubierta polimeacuterica (Zhang and Uludag 2009) (ver figura 2)
39
13Agentes estabilizadores
131 Estabilidad coloidal
El meacutetodo de doble emulsioacuten normalmente requiere la presencia de estabilizadores para
conferir estabilidad coloidal durante la primera etapa de emulsioacuten para evitar la coalescencia de
las gotas de la emulsioacuten y maacutes tarde para mantener la estabilidad de las nano micropartiacuteculas
finales (Ratzinger et al 2010) El alcohol poliviniacutelico (PVA) y el derivado de PEO como
poloxaacutemeros se han usado en la mayoriacutea de los casos (Blanco and Alonso 1998) (Feczkoacute Toacuteth
and Gyenis 2008) Otros incluyen surfactantes naturales como los fosfoliacutepidos (Feng and Huang
2001) (Chan et al 2009) En algunos casos es posible evitar los surfactantes si las partiacuteculas
tienen una contribucioacuten de estabilidad electrostaacutetica es decir de los grupos carboxilo terminales
no protegidos de las moleacuteculas de PLGA (Fraylich et al 2008)
Como se ha comentado anteriormente el PVA y los poloxaacutemeros han demostrado su eficacia
en la siacutentesis de nanopartiacuteculas y micropartiacuteculas afectando no solo la estabilidad de los sistemas
sino tambieacuten su tamantildeo y morfologiacutea Por lo tanto se ha encontrado un efecto de reduccioacuten de
tamantildeo usando PVA en la fase acuosa externa que afecta al mismo tiempo la porosidad
superficial principalmente en partiacuteculas de tamantildeo micro (Feczkoacute Toacuteth and Gyenis 2008) Un
estudio comparativo entre PVA y fosfoliacutepidos (di-palmitoil fosfatidilcolina) como estabilizadores
mostroacute que DPPC podriacutea ser un mejor emulsionante que PVA para producir nano y
micropartiacuteculas Con este meacutetodo se necesitaba una cantidad de estabilizador mucho maacutes baja
para obtener un tamantildeo similar En el mismo estudio se demostroacute una mayor porosidad en la
superficie de la partiacutecula para las nanoesferas emulsionadas con PVA (Feng and Huang 2001)
Por otro lado la combinacioacuten de PLGA con poloxaacutemeros ha mostrado efectos positivos para
los nano y microsistemas en teacuterminos de estabilidad eficacia de encapsulacioacuten o caracteriacutesticas
de liberacioacuten controlada (Santander-Ortega et al 2011) El uso de estos surfactantes en el primer
o segundo paso del procedimiento de emulsioacuten WOW conduce a situaciones diferentes Por lo
40
tanto si los poloxaacutemeros se mezclan con PLGA en la fase orgaacutenica de la emulsioacuten primaria se
obtiene una alteracioacuten de la rugosidad superficial Sin embargo si se agregan en la fase de agua
interna se encuentra un aumento de la porosidad (Blanco and Alonso 1998) La inclusioacuten de
poloxaacutemeros en la fase polar de la segunda etapa de emulsioacuten tambieacuten genera superficies de
rugosidad hidroacutefila Una cuantificacioacuten de esto se muestra en la figura 5 en la que se mide la
movilidad electroforeacutetica de PLGA puro y PLGA pluronic F68 nanopartiacuteculas como una funcioacuten
del pH del medio La composicioacuten de superficie diferente afecta el comportamiento
electrocineacutetico de las nanopartiacuteculas desnudas La carga superficial se modula por la presencia de
tensioactivo no ioacutenico como poloxaacutemeros o en mayor medida por la presencia de moleacuteculas de
anticuerpos unidos en la superficie La dependencia observada con este paraacutemetro es una
consecuencia del caraacutecter aacutecido deacutebil de los grupos carboxilo de PLGA Cuando las moleacuteculas de
poloxaacutemero estaacuten presentes en la interfaz se encuentra una reduccioacuten sistemaacutetica de la movilidad
como consecuencia del aumento de la rugosidad superficial Las cadenas de surfactante hidroacutefilo
se dispersan hacia el disolvente originando un desplazamiento del plano de corte y la consecuente
reduccioacuten de movilidad (Santander-Ortega Bastos-Gonzalez and Ortega-Vinuesa 2007)
(Fraylich et al 2008) La presencia de moleacuteculas proteicas en la superficie introduce una
dependencia de la carga neta superficial con el punto isoeleacutectrico de eacutestas situacioacuten que se refleja
en el comportamiento electrocineacutetico que incluso muestras valores positivos en pHs inferiores al
punto isoeleacutectrico del anticuerpo
El tamantildeo final de las partiacuteculas de PLGA se controla principalmente por fuerzas
electrostaacuteticas y no se ve significativamente afectado por la presencia o la naturaleza de los
estabilizadores de poloxaacutemero (Fraylich et al 2008) El reconocimiento de los nanovehiacuteculos por
el sistema fagociacutetico mononuclear (MPS) se puede alterar significativamente si la superficie de
las partiacuteculas coloidales se modifica mediante el uso de copoliacutemeros de bloque PEO de las
moleacuteculas de poloxaacutemero La barrera esteacuterica proporcionada por estas moleacuteculas surfactantes
previenen o minimizan la adsorcioacuten de proteiacutenas plasmaacuteticas y disminuye el reconocimiento por
41
los macroacutefagos (Tan et al 1993) El tamantildeo de las microesferas tampoco se ve afectado por la
encapsulacioacuten de poloxaacutemeros El sistema que contiene mezclas de poloxaacutemero-PLGA conduce
a una estructura interna que muestra pequentildeos orificios y cavidades en relacioacuten con microesferas
de PLGA puro con una estructura de tipo matriz compacta (Blanco and Alonso 1998)
Figura 5 Movilidad electroforeacutetica versus pH para nanopartiacuteculas de PLGA con diferentes
caracteriacutesticas () PLGA (◼) mezcla de PLGA poloxamer188 y () PLGA cubierto por
Immuno-γ-globulina
Las micropartiacuteculas formuladas por poloxaacutemero en el segundo medio polar tienen una
superficie completamente diferente que las de PVA casi sin poros (Feczkoacute Toacuteth and Gyenis
2008) Una comparacioacuten entre diferentes poloxaacutemeros muestra que el balance hidroacutefilo-lipoacutefilo
(HBL) del surfactante juega un papel crucial determinando las interacciones surfactante-poliacutemero
y controlando la porosidad y la rugosidad de las nano-micropartiacuteculas (Blanco and Alonso 1998)
(Bouissou et al 2004)
42
De manera similar a los surfactantes las caracteriacutesticas del poliacutemero como el grado de
hidrofobicidad el peso molecular o la velocidad de degradacioacuten de la hidroacutelisis pueden influir
fuertemente en la morfologiacutea de la partiacutecula Por lo tanto la composicioacuten polimeacuterica de las
partiacuteculas afecta en gran medida su estructura y propiedades Es por eso que es habitual usar otros
poliacutemeros para modificar el comportamiento y la aplicacioacuten de las partiacuteculas De esta manera el
polietilenglicol (PEG) de diferente longitud de cadena se usa frecuentemente para modificar las
caracteriacutesticas de la superficie Con PEG las partiacuteculas son maacutes hidroacutefilas y tienen superficies
maacutes rugosas que afectan la accioacuten de MPS al aumentar el tiempo de circulacioacuten y la vida media
in vivo como la presencia de cadenas de PEO (Gref et al 1994) Ademaacutes las cadenas de PEG
tambieacuten proporcionan estabilidad coloidal a traveacutes de la estabilizacioacuten esteacuterica Las
nanopartiacuteculas o micropartiacuteculas de PLGA se pueden obtener normalmente mediante el uso en el
meacutetodo de siacutentesis de copoliacutemeros de di y tri-bloque de PLGA PEG (Lochmann et al 2010)
(White et al 2013) (Makadia and Siegel 2011) Los poliacutemeros naturales como el quitosano
ademaacutes de aumentar la hidrofobicidad de la superficie tambieacuten les confieren un caraacutecter
mucoadherente (Paolicelli et al 2010)
132 Eficacia de encapsulacioacuten y bioactividad
Ademaacutes el uso de estabilizantes (surfactantes o poliacutemeros) tambieacuten influye en la eficacia de
encapsulacioacuten y la estabilidad de la proteiacutena De hecho para el proceso de evaporacioacuten del
solvente WOW el solvente orgaacutenico clorado usado para la primera emulsioacuten puede degradar las
moleacuteculas de proteiacutena encapsuladas en este paso si entran en contacto con la interfaz orgaacutenica
agua causando su agregacioacuten o desnaturalizacioacuten (Brigger Dubernet and Couvreur 2002) La
interaccioacuten poliacutemero-proteiacutena el estreacutes de cizallamiento para el proceso de emulsioacuten y la
reduccioacuten del pH derivada de la degradacioacuten del poliacutemero PLGA tambieacuten pueden producir la
misma situacioacuten con la peacuterdida posterior de la actividad bioloacutegica de las biomoleacuteculas
encapsuladas Se han usado diferentes estrategias para prevenirlo Por ejemplo un aumento de la
viscosidad alrededor de las moleacuteculas de proteiacutenas puede ayudar a aislarlas de su microambiente
43
(Giteau et al 2008) De esta forma los productos viscosos como el almidoacuten se han utilizado
para prevenir la inestabilidad proteica (Balmayor et al 2009) Estos autores coencapsulan BMP2
con albuacutemina dentro de micropartiacuteculas de almidoacuten usando otro poliacutemero biodegradable poli-ε-
caprolactona en lugar de PLGA La BMP2 retuvo la bioactividad A pesar de una baja tasa de
encapsulacioacuten ademaacutes de una raacutefaga inicial seguida de una liberacioacuten incompleta la cantidad de
BMP2 necesaria al principio fue menor (Balmayor et al 2009) La combinacioacuten de surfactantes
PEO con PLGA (mezclados en la fase orgaacutenica) tambieacuten puede preservar la bioactividad de las
proteiacutenas microencapsuladas (Santander-Ortega et al 2009) o los aacutecidos nucleicos (Csaba et al
2005)
Sin embargo en la mayoriacutea de los casos la estrategia preferida fue la coencapsulacioacuten de
estabilizadores con biomoleacuteculas De este modo las albuacuteminas seacutericas (SA) han demostrado la
capacidad de limitar la agregacioacuten-desestabilizacioacuten de varias proteiacutenas incitadas por el interfaz
agua disolvente orgaacutenico del proceso de emulsioacuten primaria (Meinel et al 2001) (Srinivasan et
al 2005) White y colaboradores en uno de sus estudios encapsularon lisozima dentro de
micropartiacuteculas de PLGA-PEG Ademaacutes de la funcioacuten de proteccioacuten tambieacuten observaron un
aumento importante de la eficiencia de encapsulacioacuten cuando el SA humana se co-encapsuloacute con
lisozima y BMP2 (White et al 2013) Dr Angelo y colaboradores usaron heparina como
estabilizador porque eacutesta forma un complejo especiacutefico con varios factores de crecimiento
estabiliza su estructura tridimensional y promueve su bioactividad Se consiguioacute aumentar asiacute la
eficacia de encapsulacioacuten del 35 al 87 usando SA bovina como un segundo estabilizador para
encapsular dos factores de crecimiento proangiogeacutenico naturales dentro de las nanopartiacuteculas
mezcladas con PLGA-poloxaacutemero Los ensayos celulares in vitro mostraron la preservacioacuten de la
actividad bioloacutegica de GF hasta un mes despueacutes (drsquoAngelo et al 2010)
El uso de maacutes surfactantes hidroacutefilos (poloxaacutemeros) o poliacutemeros (PEG) en la fase acuosa
interna o durante la mezcla del PLGA en la fase orgaacutenica de la emulsioacuten primaria reduce la
interaccioacuten de las proteiacutenas encapsuladas con la matriz de PLGA hidroacutefoba Esto evita la
44
alteracioacuten de la estructura de las moleacuteculas de proteiacutena y ayuda al mismo tiempo a neutralizar la
acidez generada por la degradacioacuten hidroliacutetica del PLGA (Tan et al 1993) En algunos casos se
ha demostrado que la combinacioacuten de varios estabilizadores como poloxaacutemeros tranexaacutemico y
bicarbonato de sodio preserva la integridad de las proteiacutenas encapsuladas pero tambieacuten reduce
la eficacia de la encapsulacioacuten (Bouissou et al 2004)
Como regla general la eficacia de la encapsulacioacuten aumenta con el tamantildeo de las partiacuteculas
(Hans and Lowman 2002) Ademaacutes la estabilizacioacuten adecuada de la emulsioacuten primaria por
poliacutemeros anfifiacutelicos y una solidificacioacuten (precipitacioacuten) raacutepida del poliacutemero en el segundo paso
son paraacutemetros favorables para mejorar la eficacia de encapsulacioacuten de proteiacutenas en la teacutecnica de
emulsioacuten WOW (Meng et al 2003)
La tendencia de BMP2 a interactuar con superficies hidrofoacutebicas puede disminuir la peacuterdida
de proteiacutena encapsulada durante la liberacioacuten de la fase de disolvente Esto favorece una mayor
encapsulacioacuten pero disminuye la liberacioacuten posterior (Lochmann et al 2010) Se obtiene una
encapsulacioacuten proteica oacuteptima cuando el pH de las fases de agua internas y externas estaacuten cerca
del punto isoeleacutectrico de la proteiacutena (Manuel J Santander-Ortega Lozano-Loacutepez et al 2010)
Blanco y Alonso (Blanco and Alonso 1998) observaron una reduccioacuten en la eficacia de
encapsulacioacuten de proteiacutena cuando el poloxaacutemero se coencapsuloacute en la emulsioacuten primaria Esto
pone de relieve el papel principal desempentildeado por la interaccioacuten proteiacutena-poliacutemero en la eficacia
de encapsulacioacuten y el proceso de liberacioacuten posterior Sin embargo demasiado emulsionante
tambieacuten puede dar como resultado una reduccioacuten de la eficacia de encapsulacioacuten (Feng and
Huang 2001) Por lo tanto se necesita un equilibrio entre el polvo de emulsioacuten del surfactante y
su concentracioacuten
14 Patroacuten de liberacioacuten
El patroacuten de liberacioacuten representa una de las caracteriacutesticas maacutes importantes de un sistema
portador de partiacuteculas nano micro ya que su desarrollo tiene un objetivo final principal la
45
liberacioacuten adecuada de las moleacuteculas bioactivas encapsuladas para alcanzar la accioacuten cliacutenica
deseada
Se han definido diferentes patrones de liberacioacuten de proteiacutenas encapsuladas en micropartiacuteculas
nanopartiacuteculas de PLGA Es posible encontrar una liberacioacuten continua cuando la difusioacuten de la
biomoleacutecula es maacutes raacutepida que la erosioacuten de la partiacutecula Este proceso implica una difusioacuten
continua de la proteiacutena que se encuentra en la matriz del poliacutemero antes de que la partiacutecula de
PLGA se degrade en monoacutemeros de aacutecido laacutectico y glicoacutelico por hidroacutelisis (Kumari Yadav and
Yadav 2010) Tambieacuten se ha descrito una liberacioacuten bifaacutesica caracterizada por una descarga
inicial dentro o cerca de la superficie de la partiacutecula seguido por una segunda fase en la que la
proteiacutena se libera progresivamente por difusioacuten La segunda fase se puede mejorar mediante la
erosioacuten masiva del caparazoacuten y la matriz de PLGA lo que da como resultado un importante
aumento de poros y canales (Makadia and Siegel 2011) Se ha encontrado un tercer perfil de
liberacioacuten trifaacutesica cuando se produce un periacuteodo de liberacioacuten de retardo despueacutes de la descarga
inicial y hasta la degradacioacuten del poliacutemero (Cleland 1997) Finalmente es posible obtener una
liberacioacuten de proteiacutena incompleta como consecuencia de factores adicionales relacionados con la
interaccioacuten proteiacutena-poliacutemero o la inestabilidad proteica La Figura 6 ilustra los diferentes perfiles
de liberacioacuten descritos anteriormente Un sistema de transporte oacuteptimo deberiacutea ser capaz de liberar
un gradiente de concentracioacuten controlado de factores de crecimiento en el momento apropiado
evitando o al menos reduciendo o controlando el efecto de descarga inicial (Oh Kim and Lee
2011) Una explosioacuten inicial controlada seguida de una liberacioacuten sostenida mejora
significativamente la regeneracioacuten oacutesea in vivo (Brown et al 2009) (Brown et al 2011) (Li et
al 2009)
Giteau y colaboradores (Giteau et al 2008) presentan una revisioacuten interesante sobre Coacutemo
lograr una liberacioacuten sostenida y completa de micropartiacuteculas de PLGA Comienzan por analizar
la influencia del medio de liberacioacuten y el meacutetodo de muestreo en el perfil de liberacioacuten y resaltan
la importancia del proceso de limpieza por centrifugacioacuten o el volumen del medio de liberacioacuten
46
Ajustando a valores adecuados la velocidad de centrifugacioacuten o el volumen del tampoacuten es posible
separar micro nanopartiacuteculas del medio de liberacioacuten que contiene proteiacutenas de una manera muy
faacutecil Esto permite patrones de liberacioacuten estables y reproducibles Por otro lado para garantizar
un mejor perfil de liberacioacuten de proteiacutenas se debe realizar la modificacioacuten de la formulacioacuten de
micropartiacuteculas y el proceso de microencapsulacioacuten para preservar la agregacioacuten de proteiacutenas La
estabilidad de la proteiacutena debe mantenerse evitando la formacioacuten de un medio dantildeino Por
ejemplo la formulacioacuten de una siacutentesis en concreto puede modificarse para usar poliacutemeros maacutes
hidroacutefilos ya que se ha demostrado que reducen el estallido inicial y proporcionan proteiacutenas
bioactivas durante largos periodos de tiempo
47
Figura 6 Patroacuten de liberacioacuten (liacutenea negra) Cineacutetica de liberacioacuten de BSA en nanopartiacuteculas
de PLGA con alta liberacioacuten inicial (liacutenea de puntos rojos) modelo bifaacutesico que combina un
estallido inicial moderado y una liberacioacuten sostenida posterior (liacutenea de trazos azules) modelo
trifaacutesico con un retraso de liberacioacuten entre las fases de liberacioacuten inicial y sostenida (liacutenea verde
de guiones) liberacioacuten incompleta
Las estrategias maacutes relevantes se mencionan a continuacioacuten La liberacioacuten de faacutermaco a partir
de nano micropartiacuteculas de PLGA puede controlarse por el peso molecular del poliacutemero y la
relacioacuten entre monoacutemeros (laacutectico glicoacutelico) de forma que un aumento del aacutecido glicoacutelico
acelera la peacuterdida de peso del poliacutemero debido a la mayor hidrofilicidad de la matriz (Makadia
and Siegel 2011)
Por otro lado se ha descrito una erosioacuten maacutes raacutepida de las microesferas con reduccioacuten en el
peso molecular de PLGA debido a la facilidad de penetracioacuten del agua y la posterior degradacioacuten
del poliacutemero (Blanco and Alonso 1998) Schrier y colaboradores trabajaron con microesferas
48
preparadas por WOW utilizando diferentes tipos de PLGA analizaron el importante papel del
peso molecular la relacioacuten laacutectico-glicoacutelico y los residuos de aacutecido (Schrier et al 2001) La
cantidad de rhBMP2 adsorbido en la superficie de la micropartiacutecula aumentoacute con la
hidrofobicidad del poliacutemero Al mismo tiempo la liberacioacuten estaba en correlacioacuten con el perfil
de degradacioacuten de los diferentes poliacutemeros (Schrier et al 2001)
Por lo tanto el uso de poliacutemeros maacutes hidroacutefilos reduce la interaccioacuten proteiacutena hidroacutefoba-
poliacutemero Este efecto favorece una distribucioacuten maacutes homogeacutenea en la matriz polimeacuterica y
aumenta la absorcioacuten de agua en las microesferas Por lo que la velocidad de liberacioacuten de
rhBMP2 encapsulada en microesferas compuestas por un copoliacutemero de di-bloque PEG-PLGA
se incrementa con el contenido de PEG de la matriz de poliacutemero (Lochmann et al 2010) Se
obtuvo un resultado similar utilizando copoliacutemeros tri-bloque PLGA-PEG-PLGA (White et al
2013) En este caso modificando la relacioacuten del monoacutemero (laacutectido-glicoacutelido) en el PLGA y
aumentando la cantidad de PLGA-PEG-PLGA en las formulaciones el patroacuten de liberacioacuten de
BMP-2 co-encapsulada con HSA en microesferas fue ajustable
Por otro lado el uso de mezclas de PLGA-poloxaacutemeros es uacutetil para obtener una liberacioacuten
sostenida durante maacutes de un mes sin incidencia de producirse una descarga inicial (drsquoAngelo et
al 2010) (Manuel J Santander-Ortega Lozano-Loacutepez et al 2010) Sin embargo para un
plaacutesmido encapsulado dentro de nanopartiacuteculas obtenidas mediante mezclas de PLGA-
poloxaacutemero la hidrofobicidad del surfactante permite prolongar la liberacioacuten hasta 2 semanas de
una manera controlada Por otra parte se alcanzoacute una liberacioacuten completa para la mezcla de
PLGA-poloxaacutemero en lugar de nanopartiacuteculas de PLGA en la que la liberacioacuten maacutexima fue de
alrededor del 40 (Csaba et al 2005)
Las mezclas de PLGA y poloxaacutemeros (pluronic F68) tambieacuten se pueden usar para obtener
vesiacuteculas o caacutemaras nanocompuestas mediante un proceso de doble emulsioacuten Estas vesiacuteculas son
adecuadas para la encapsulacioacuten de moleacuteculas hidrofoacutebicas e hidrofiacutelicas La presencia de
pluronic afecta la estabilidad coloidal de las vesiacuteculas y el patroacuten de liberacioacuten de las moleacuteculas
49
encapsuladas Estas vesiacuteculas presentan una pared de 30 nm y el faacutermaco estaacute encapsulado en
presencia del poloxaacutemero (Nair and Sharma 2012)
Otras estrategias incluyen el uso de diferentes compuestos para aumentar el tiempo de
liberacioacuten Por lo tanto BMP2 encapsulada en nanopartiacuteculas de PLGA-PVA (alrededor de 300
nm) mostroacute una mayor eficiencia de encapsulacioacuten y un perfil de liberacioacuten de corta duracioacuten con
un estallido inicial muy alto De forma similar las nanopartiacuteculas de mezcla PLGA-poloxaacutemero
se modificaron superficialmente introduciendo quitosano en el segundo paso de la siacutentesis Este
meacutetodo mostroacute un perfil de liberacioacuten sostenida de hasta 14 diacuteas sin ninguacuten estallido importante
inicial En este caso se utilizoacute un antiacutegeno recombinante de hepatitis B (Paolicelli et al 2010)
Ademaacutes el uso de heparina conjugada con microesferas porosas de PLGA tambieacuten se ha descrito
para obtener un sistema de administracioacuten a largo plazo que reduce al mismo tiempo el estallido
inicial En estos sistemas la heparina se inmovilizoacute en la superficie nano micropartiacutecula La
liberacioacuten se controloacute usando las afinidades de unioacuten de la heparina a varios factores de
crecimiento incluida la BMP2 En este caso el estallido inicial se redujo hasta un 4-7 durante
el primer diacutea seguido de una liberacioacuten sostenida de aproximadamente un 1 por diacutea (La et al
2010) (Chung et al 2007) (Jeon et al 2008)
La liberacioacuten de proteiacutena en la descarga inicial puede atenuarse mediante la fabricacioacuten de
microesferas de doble pared es decir micropartiacuteculas con nuacutecleo y concha La presencia de un
revestimiento o armazoacuten de PLA reduce la tasa de liberacioacuten de BSA encapsulado en el nuacutecleo
PLGA y extiende la duracioacuten del perfil de liberacioacuten hasta dos meses Por otra parte un aumento
en el peso molecular de PLA influye en la tasa de erosioacuten de las partiacuteculas lo que ralentiza auacuten
maacutes la liberacioacuten de proteiacutenas (Xia et al 2013)
La modificacioacuten de la viscosidad en el entorno de micropartiacuteculas influye adicionalmente en
el patroacuten de liberacioacuten La viscosidad puede controlar el estallido en el punto maacutes temprano y
promover una liberacioacuten sostenida Esta situacioacuten se ha demostrado para las microesferas de
rhBMP2-PLGA incrustadas en un hidrogel de aacutecido quitosano-tioglicoacutelico (Poloxaacutemero 407) (Fu
50
et al 2012) Yilgor y colaboradores tambieacuten incorporaron las nanopartiacuteculas de su sistema de
administracioacuten secuencial a un scaffolds compuesto por quitosano y quitosano-PEO (Yilgor et al
2009) En otro trabajo las microesferas de PLGA PVA con BMP2 encapsulado se combinaron
con diferentes biomateriales compuestos (hidrogel de gelatina o fumarato de polipropileno) La
liberacioacuten sostenida de la moleacutecula bioactiva se extendioacute durante un periacuteodo de 42 diacuteas Los
resultados in vivo indican la importancia de las caracteriacutesticas compuestas En este caso se obtuvo
una mejor formacioacuten de hueso cuando las micropartiacuteculas de PLGA se incorporaron a la matriz
maacutes hidroacutefoba (fumarato de polipropileno) (Kempen et al 2008) (Kempen et al 2009)
Finalmente la tabla 1 resume informacioacuten importante sobre diferentes paraacutemetros relacionados
con el uso de nano o micropartiacuteculas basadas en PLGA para encapsular transportar y liberar
factores de crecimiento (principalmente BMP2) La mayoriacutea de ellos estaacuten en la escala
microscoacutepica El PVA ha sido el estabilizador de surfactante maacutes utilizado Es posible encontrar
tanto la encapsulacioacuten como la adsorcioacuten de superficie de los factores de crecimiento con una
eficiencia alta a moderada El uso de heparina como estabilizador reduce significativamente la
liberacioacuten inicial en estallido favoreciendo una liberacioacuten sostenida en el tiempo La bioactividad
del GF se conservoacute en la mayoriacutea de los sistemas y la encapsulacioacuten con otras biomoleacuteculas parece
tener un efecto similar al del uso de surfactantes como estabilizadores
51
TABLA 1 Sistemas de nano micropartiacuteculas para encapsular GF principalmente el factor
de crecimiento BMP2
Poliacutemeros Estabilizadores Tamantildeo EE
Encapsulacioacuten Liberacioacuten
Actividad
bioloacutegica Referencia
PLGA PVA 10-20 microm Adsorcioacuten
rhBMP2
20 ngml de
contasnte
liberacioacuten
sostenida
Mejor formacioacuten
oacutesea 8 semanas
despueacutes
Fu at al 2012
(126)
PLGA PVA 10-100
μm
rhBMP2-BSA
69 (BMP)
Burst (20 )
Prolongado
hasta un 77
(28 diacuteas)
Moleacuteculas de
BMP2 con
bioactividad
Tian et al
2012
(130)
PLGA 7525 PVA 182 μm 82 -
Buenos resultados
de reparacioacuten oacutesea
de 8 a 12 semanas
Rodriguez-
Evora et al
2014 (130)
PLGA PVA 228 μm 605
30 en la
descarga inicial
Liberacioacuten maacutes
lenta de un 4
por semana
Despueacutes de 8
semanas
liberado un
60
Sin peacuterdida de
bioactividad
Reyes et al
2013 (132)
PLGAPEG Sin siacutentesis de
doble emulsion
100-200
μm Adsorcioacuten BMP2
13 en la
descarga inicial
Liberacioacuten mas
lenta de 001-8
por dia
Despueacutes de 23
diacuteas liberado
un 70
Sustancial
regeneracioacuten oacutesea
debido al
ldquoandamiordquo
Rahman et al
2014 (181)
PLGA Diferente PVA 20-100
μm
30 (sin cubrir
PLGA)
90 (cubriendo
PLGA)
26-49 (1 dia)
Total 2 semanas
despueacutes
Sin peacuterdida de
bioactividad
Lupu-Haber
et al 2013
(134)
PLGA 7525 PVA 5-125 μm -
Descarga inicial
de un 30 (1
diacutea)
Prolongada 35
diacuteas
Mayores
voluacutemenes y
cobertura de
superficie del
Nuevo hueso
Wink et al
2014
(138)
PLGA Heparina 200-800
nm
Adsorcioacuten BMP2
94
Sin descarga
inicial
Prolongado 4
semanas
Reduccioacuten
significativa de la
dosis de BMP2
para una Buena
formacioacuten oacutesea
La et al 2010
(122)
PLGA Heparina-
poloxaacutemero 160 nm
Adsorcioacuten BMP2
100
Descarga inicial
(4-7) perfil
lineal
Mayor
mineralizacioacuten de
la matriz del hueso
regenerado
Chung et al
2007 (123)
PLGA Heparina 100-250
nm Adsorcioacuten 94
Descarga inicial
10 (1 dia)
60 30 diacuteas
despueacutes
Sin peacuterdida de
bioactividad
Eficacia de la
administracioacuten
cantidad 50 veces
menor
Jeon et al
2008 (124)
PLGA PVA ~ 300 nm 80 85 descarga
inicial (1 dia)
Sin peacuterdida de
bioactividad
Yilgor et al
2009 (127)
PLGA (en anillos) PVA 215 μm 66 Descarga El 60 de los Rodriguez-
52
15 Vectorizacioacuten Entrega dirigida
Las nano-esferas de PLGA representan un sistema de administracioacuten de biomoleacuteculas bien
estudiado que podriacutea aplicarse a la seleccioacuten celular con el fin de mejorar el suministro de
proteiacutenas especiacuteficas o aacutecidos nucleicos dentro o cerca de las ceacutelulas de referencia de ingenieriacutea
oacutesea es decir ceacutelulas madre mesenquimales (Vo Kasper and Mikos 2012) Las propiedades de
direccionamiento pueden ser suministradas por una estrategia de funcionalizacioacuten del ligando
modificacioacuten de la estructura superficial del nano-transportador conjugando un ligando especiacutefico
de ceacutelula para dirigir la liberacioacuten de biomoleacuteculas encapsuladas preferiblemente en estrecha
asociacioacuten con las ceacutelulas diana (Ji et al 2012) El uso de nanopartiacuteculas con una unioacuten covalente
de diferentes ligandos da lugar a una teacutecnica potencial para administrar biomoleacuteculas especiacuteficas
de ceacutelulas oacuteseas para la ingenieriacutea oacutesea (Luginbuehl et al 2004)
Los anticuerpos especiacuteficos que reconocen los receptores de superficie en estas ceacutelulas podriacutean
acoplarse covalentemente a la superficie de las nanopartiacuteculas de PLGA obteniendo inmuno-
nanopartiacuteculas Hay varios ejemplos de inmovilizacioacuten de anticuerpos en la superficie de
nanopartiacuteculas de PLGA Kocbek y colaboradores demostraron el reconocimiento especiacutefico de
las ceacutelulas tumorales de mama por un anticuerpo monoclonal especiacutefico unido a las nanopartiacuteculas
fluorescentes PLGA obtenidas mediante el proceso de emulsioacuten WOW (Kocbek et al 2007)
Para la unioacuten covalente de la superficie utilizaron un meacutetodo de carbodimida maacutes simple que
promueve la formacioacuten de un enlace amida entre los grupos terminales carboxiacutelicos libres de
nanopartiacuteculas de PLGA y los grupos amino primarios de la moleacutecula del anticuerpo (Ertl et al
2000) Este procedimiento puede verse muy influenciado por la presencia de estabilizadores
frecuentemente utilizados para conferir estabilidad coloidal a las nanopartiacuteculas La movilidad
electroforeacutetica de las nanopartiacuteculas de PLGA con un anticuerpo (inmuno-γ-globulina anti-
proteiacutena C-reactiva humana) unido covalentemente a la superficie como se muestra en la figura
5 Es necesario observar la draacutestica disminucioacuten en los valores de movilidad del anticuerpo
modificado con respecto a las nanopartiacuteculas de PLGA desnudas lo que podriacutea implicar una baja
53
estabilidad coloidal y la posterior agregacioacuten del nanosistema Santander y colaboradores
propusieron una menor carga de anticuerpos en la que las nanoparticulas de PLGA desnudas
deben ser recubiertas por un agente surfactante no ioacutenico con el fin de obtener nanopartiacuteculas
estables inmunorrectivas (Santander-Ortega Bastos-Gonzalez and Ortega-Vinuesa 2007)
Ratzinger y colaboradores indicaron que la presencia de altas concentraciones de poloxaacutemero
disminuyoacute la eficacia de acoplamiento a grupos carboxiacutelicos en nanopartiacuteculas de PLGA lo que
demuestra que es necesario un equilibrio que combine maacutes estabilidad y mejor eficacia de
acoplamiento (Ratzinger et al 2010) Para evitar este problema Cheng y colaboradores
sintetizaron el copoliacutemero en bloque de PLGA-PEG funcionalizado con carboxilo uniendo un
aptaacutemero especiacutefico a la superficie de las nanopartiacuteculas pegiladas mediante el meacutetodo de la
carbodiimida En este trabajo se ha demostrado una mejor administracioacuten de faacutermacos en los
tumores de proacutestata en comparacioacuten con las nanopartiacuteculas equivalentes no dirigidas (Cheng et
al 2007)
16 Ingenieriacutea tisular soportes 3D o ldquoscaffoldsrdquo
Los datos publicados en la literatura indican que las nanopartiacuteculas micropartiacuteculas de PLGA
prometen lograr una administracioacuten sostenida espacial y temporalmente controlada por factores
de crecimiento requeridos para el desarrollo celular y la diferenciacioacuten celular Se pueden
incorporar a ceacutelulas en scaffolds soacutelidos o hidrogeles inyectables (Danhier et al 2012) Los
scaffolds o andamios son estructuras 3D porosas que normalmente se utilizan para mejorar la
ingenieriacutea tisular oacutesea (A C Carreira et al 2014) De acuerdo con Tian y colaboradores (Tian et
al 2012) un scaffolds disentildeado con este objetivo debe tener (1) resistencia mecaacutenica apropiada
para soportar el crecimiento de hueso nuevo (2) porosidad apropiada para permitir el crecimiento
de las ceacutelulas relacionadas con los huesos (3) buena biocompatibilidad que permite el crecimiento
de ceacutelulas en su superficie sin ser rechazado por el cuerpo (4) baja toxicidad para las ceacutelulas y
tejidos de alrededor (5) ser capaz de inducir la diferenciacioacuten osteogeacutenica de las ceacutelulas madre
54
relacionadas con los huesos (6) ser biodegradable con productos de degradacioacuten no toacutexicos para
que eventualmente puedan ser reemplazados por nuevo hueso Ademaacutes el scaffolds para la
regeneracioacuten oacutesea debe mantener el suministro o la liberacioacuten de BMP (factores de crecimiento)
in situ durante un tiempo prolongado De esta forma las nano micropartiacuteculas dentro de los
scaffolds se utilizan para liberar un flujo adecuado de estas biomoleacuteculas de sentildealizacioacuten y
preservar su estructura funcional (Romagnoli DrsquoAsta and Brandi 2013) Para hacerlo se requiere
una liberacioacuten inicial del factor de crecimiento encapsulado en las primeras horas para poder
obtener raacutepidamente una concentracioacuten terapeacuteutica efectiva seguida de un perfil de liberacioacuten
sostenido a largo plazo (Puppi et al 2014) La mayoriacutea de las partiacuteculas polimeacutericas insertadas
en las estructuras de los scaffolds estaacuten en una escala micromeacutetrica El objetivo principal de estas
micropartiacuteculas es la proteccioacuten y el control temporal de la entrega del factor de crecimiento Sin
embargo dada la porosidad de estas estructuras las nanopartiacuteculas y especialmente las partiacuteculas
de algunas micras pueden volverse maacutes importantes ya que es posible disentildear sistemas con una
difusioacuten simple y faacutecil a traveacutes de la estructura Este proceso podriacutea permitir el reconocimiento
especiacutefico de un tipo de ceacutelula particular liberando sus BMP encapsuladas en el mismo entorno
y ayudando a su diferenciacioacuten al tejido celular oacuteseo
55
2 HIPOacuteTESIS
Lo que sabemos hasta ahora es que las BMPs y especiacuteficamente la BMP-2 son muy uacutetiles
para promover la regeneracioacuten oacutesea induciendo una mayor formacioacuten de hueso de la zona
receptora de igual calidad que el hueso nativo del paciente Sin embargo la administracioacuten local
presenta algunas limitaciones como que la proteiacutena se puede inactivar muy raacutepidamente y la
distribucioacuten de la BMP en una suspensioacuten liacutequida hace que sea imposible estar seguro de que la
proteiacutena haya alcanzado el objetivo Para ayudar a resolver estos problemas planteamos la
siguiente hipoacutetesis
HIPOTESIS CIERTA La encapsulacioacuten de BMP-2 con nuestras nanopartiacuteculas permiten una
administracioacuten localizada un transporte especifico y una liberacioacuten controlada de la biomoleacutecula
mejorando asiacute su farmacocineacutetica y farmacodinamia y disminuyendo los efectos secundarios
derivados de esta
HIPOacuteTESIS NULA Que tras la experimentacioacuten no seamos capaces de promover la
encapsulacioacuten de la BMP-2 dentro de nuestras nanopartiacuteculas de PLGA o que producieacutendose no
se produzca la administracioacuten de BMP-2 localizada y liberacioacuten prolongada o que nuestro sistema
de encapsulacioacuten pueda presentar efectos citotoacutexicos a nivel celular
56
3OBJETIVOS
31 Objetivo principal
Optimizar la formulacioacuten de diferentes tipos de nanopartiacuteculas de PLGA para el transporte y
suministro de BMP-2 que permita conseguir una cineacutetica de liberacioacuten controlada aumentando la
vida media de este factor de crecimiento oacuteseo y preservando su accioacuten bioloacutegica
32 Objetivos secundarios
Nos planteamos los siguientes
1 Llevar a cabo la siacutentesis de NPs polimeacutericas de PLGA mediante el procedimiento de
doble emulsioacuten para conseguir nanosistemas coloidal y temporalmente estables
2 Modelar los procedimientos de siacutentesis de NPs de PLGA para la encapsulacioacuten o carga
de moleacuteculas proteicas en la proporcioacuten adecuada sin alterar su actividad bioloacutegica
3 Desarrollar una completa caracterizacioacuten quiacutemico-fiacutesica de los diferentes nanosistemas
polimeacutericos en ausencia de proteiacutena y con carga de la misma haciendo hincapieacute en la
interaccioacuten surfactante-proteiacutena y analizando sus propiedades superficiales y
coloidales
4 Desarrollar una caracterizacioacuten bioloacutegica de los diferentes nanosistemas con proteiacutena
centrada en el anaacutelisis de la cineacutetica de liberacioacuten proteica y su actividad bioloacutegica
5 Evaluar in vitro las interacciones celulares citotoxicidad y captacioacuten celular de los
diferentes sistemas de NPs de PLGA en ceacutelulas estromales mesenquimales de hueso
alveolar humano
6 Tomando como punto de partida el modelo de NPs con proteiacutena con las mejores
propiedades coloidales y bioloacutegicas formular las condiciones oacuteptimas para obtener un
nanotransportador de partiacuteculas de PLGA cargado con BMP2
57
7 Desarrollar una completa caracterizacioacuten quiacutemico-fiacutesica y bioloacutegica de las NPs con
BMP2 analizando sus propiedades superficiales coloidales y la cineacutetica de liberacioacuten
proteica
8 Analizar la actividad bioloacutegica de la BMP-2 vehiculizada mediante las NPs de PLGA
mediante experiencias de proliferacioacuten migracioacuten y diferenciacioacuten osteogeacutenica en
ceacutelulas estromales mesenquimales de hueso alveolar humano
58
4NANOSISTEMA DE ENTREGA DOBLE PARA BIOMOLEacuteCULAS
FORMULACIOacuteN CARACTERIZACIOacuteN Y LIBERACIOacuteN IN
VITRO
41Antecedentes
La regeneracioacuten tisular es una accioacuten bioloacutegica compleja que implica muacuteltiples pasos de forma
secuencial ordenada y controlada (Padial-Molina et al 2012) (Padial-Molina Rodriguez et al
2015) Claacutesicamente se han propuesto moleacuteculas bioactivas para ayudar en estos procesos Sin
embargo el uso de altas dosis la desnaturalizacioacuten y la peacuterdida de actividad bioloacutegica el tiempo
de accioacuten descontrolado y la difusioacuten a otros tejidos destacan como los principales problemas de
esta estrategia terapeacuteutica (Ortega-Oller et al 2015) Para ayudar a resolver estas dificultades en
los uacuteltimos antildeos se ha investigado intensamente la nanomedicina como un aacuterea emergente Esto
implica meacutetodos de diagnoacutestico terapeacuteuticos y de regeneracioacuten mediante estructuras y sistemas
en los que el tamantildeo y la forma se controlan a nivel atoacutemico molecular y supramolecular (Ki-
Bum Lee Ani Solanki J Dongun Kim 2009) El transporte y la administracioacuten controlada de
faacutermacos yo biomoleacuteculas terapeacuteuticas mejora su farmacocineacutetica y farmacodinaacutemica y al
mismo tiempo minimiza los efectos secundarios nocivos Para estos propoacutesitos se describieron
diferentes tipos de nanosistemas El aacutecido polilaacutectico-co-glicoacutelico (PLGA) exhibe una baja
citotoxicidad asi como una alta biocompatibilidad y biodegradabilidad con las liberaciones de
subproductos no toacutexicos
En la uacuteltima deacutecada se ha investigado el uso de PLGA para administrar un amplio espectro de
agentes activos desde moleacuteculas de faacutermacos hidroacutefobas (Yallapu et al 2010) (Nair and Sharma
2012) (Shankarayan Kumar and Mishra 2013) a biomoleacuteculas hidroacutefilas como peacuteptidos
(Loureiro et al 2016) proteiacutenas (Blanco and Alonso 1998) (Perez De Jesus and Griebenow
2002) (Manuel J Santander-Ortega Csaba et al 2010) (Pirooznia et al 2012) (drsquoAngelo et al
2010) (White et al 2013) o aacutecidos nucleicos (Pantazis et al 2012) (Park et al 2013) Estos
59
sistemas de entrega se han producido a traveacutes de diferentes procesos de formulacioacuten para su
aplicacioacuten en terapias tanto sisteacutemicas como locales especiacuteficas del sitio (Wan and Yang 2016)
Sin embargo su disentildeo y desarrollo como nanotransportadores son difiacuteciles debido al patroacuten
de liberacioacuten problemaacutetico que presentan cuando las moleacuteculas encapsuladas son proteiacutenas para
las cuales las descargas iniciales y la liberacioacuten lenta o incompleta podriacutean ser un problema (Wan
and Yang 2016) (Giteau et al 2008) (Fredenberg et al 2011) Ademaacutes las condiciones
especiacuteficas de la liberacioacuten pueden necesitar ser diferentes dependiendo de la aplicacioacuten final del
nanotransportador (Fredenberg et al 2011) (Mohamed and van der Walle 2008)
La teacutecnica de doble emulsioacuten de aguaaceiteagua (WOW) es el meacutetodo de encapsulacioacuten de
proteiacutenas maacutes ampliamente utilizado para PLGA en micro (MP) y nanopartiacuteculas (NP) (Csaba et
al 2004) (Makadia and Siegel 2011) Permite modular diferentes factores como el tipo de PLGA
el uso de otros poliacutemeros mezclados con PLGA la adicioacuten de surfactantes el estreacutes mecaacutenico o
el solvente orgaacutenico (Fredenberg et al 2011) Tambieacuten es posible construir varios tipos de
copoliacutemeros para modificar la hidrofobicidad la relacioacuten de hidrofilicidad (Wan and Yang 2016)
(Danhier et al 2012) y la estabilidad el tamantildeo y el proceso de liberacioacuten coloidal El par PLGA
polietilenglicol y los surfactantes como el alcohol poliviniacutelico (PVA) o los oacutexidos de polietileno
(PEO) son los maacutes ampliamente estudiados (Nair and Sharma 2012) (Manuel J Santander-
Ortega Csaba et al 2010) (Ratzinger et al 2010) (Meng et al 2003)
Por otro lado la ingenieriacutea de tejidos requiere la participacioacuten de ceacutelulas estromales
mesenquimales (MSC) (Padial-Molina OrsquoValle et al 2015) Se sabe que las MSC tienen la
capacidad de diferenciarse en muacuteltiples tipos de ceacutelulas incluidos los osteoblastos Los
osteoblastos son las ceacutelulas principales responsables de sintetizar el tejido oacuteseo mineralizado Este
proceso estaacute regulado por entre otras moleacuteculas BMP-2 (Ortega-Oller et al 2015) Las
partiacuteculas de PLGA cargadas con BMP-2 son sistemas ampliamente utilizados como se ha
descrito y revisado por otros autores (Ortega-Oller et al 2015) (Yilgor Hasirci and Hasirci 2010)
(Li et al 2009) (Wang et al 2015) (Shim et al 2016)
60
Asiacute dentro de este contexto el objetivo del presente estudio fue optimizar la formulacioacuten y
propiedades de un sistema de nanopartiacuteculas con gran variedad de aplicaciones terapeacuteuticas
Probamos dos estrategias diferentes para obtener NP de tensioactivo PLGA utilizando lisozima
como modelo para BMP-2 Analizamos el tamantildeo y la morfologiacutea el iacutendice de polidespersidad
el potencial zeta la estabilidad coloidal y la eficiencia de encapsulacioacuten (EE) de la proteiacutena
Una vez finalizada la caracterizacioacuten fiacutesico-quiacutemica el estudio se centroacute en el proceso de
liberacioacuten de proteiacutenas utilizando diferentes teacutecnicas para estudiar los resultados de experimentos
in vitro y centraacutendose en el patroacuten de liberacioacuten y la actividad bioloacutegica de la lisozima liberada
De esta manera se establecioacute una nueva formulacioacuten para desarrollar un nanosistema de PLGA
con una distribucioacuten de tamantildeo dual singular y el equilibrio adecuado entre encapsulacioacuten y
liberacioacuten de proteiacutenas bioloacutegicamente activas Finalmente los efectos del sistema PLGA
propuesto se probaron en MSC primarias in vitro como prueba del nuevo sistema desarrollado
42Materiales y meacutetodos
421Formulacioacuten de las nanoparticulas
El aacutecido poli (laacutectico-co-glicoacutelico) (PLGA 5050) ([C2 H2 O2]x [C3 H4 O2]y) x = 50 y = 50
(Resomerreg 503H) 32 - 44 kDa se usoacute como poliacutemero El surfactante polimeacuterico Pluronicreg F68
(Poloxaacutemero 188) (Sigma-Aldrich) se usoacute como el emulsionante Su estructura se basa en un
copoliacutemero tri-bloque de poli (oacutexido de etileno) PEO y poli (oacutexido de propileno) PPO (PEO)a ndash
(PPO)b ndash (PEO)a con a = 75 y b = 30 La lisozima de huevo de gallina (Sigma-L7651) se usoacute
como proteiacutena hidroacutefila El agua se purificoacute en un sistema Milli-Q Academic de Millipore Se
desarrollaron dos meacutetodos de formulacioacuten diferentes denominados O-F68 y W-F68
En el meacutetodo O-F68 se disolvieron 25 mg de PLGA y 15 mg de F68 en 660 120583119871 de
diclorometano (DMC) y se agitaron en el vortex Luego se antildeadieron 330 micro-litros de acetona
y se agitaron en el vortex tambieacuten A continuacioacuten se antildeadieron 100 120583119871 de una solucioacuten
tamponada a pH 128 con o sin lisozima (5 mg mL) gota a gota mientras se agita en vortex
61
durante 30 s Inmediatamente esta emulsioacuten primaria de agua aceite (WO) se vertioacute en un vidrio
que conteniacutea 125 ml de etanol bajo agitacioacuten magneacutetica y se antildeadieron 125 ml de agua MilliQ
Despueacutes de 10 minutos de agitacioacuten magneacutetica los disolventes orgaacutenicos se extrajeron
raacutepidamente por evaporacioacuten al vaciacuteo hasta que la muestra alcanzoacute un volumen final de 10 ml
En el meacutetodo W-F68 se disolvieron 100 mg de PLGA en un tubo que conteniacutea 1 ml de acetato
de etilo (EA) y se agitaron en vortex Se antildeadieron 40 120583119871 de una solucioacuten tamponada a pH 128
con o sin lisozima (20 mg ml) e inmediatamente se sonicaron (Branson Ultrasonics 450 Analog
Sonifier) fijando el debido ciclo de trabajo al 20 y de control de salida a 4 durante 1 min con
el tubo rodeado de hielo Esta emulsioacuten primaria WO se vertioacute en un tubo de plaacutestico que conteniacutea
2 ml de una solucioacuten tamponada (pH 128) de F68 a 1 mg ml y se agitoacute en voacutertex durante 30 s
Luego el tubo rodeado de hielo se sonicoacute de nuevo a la maacutexima amplitud para la micro punta
(control de salida 7) durante 1 minuto Esta segunda emulsioacuten WOW se vertioacute en un vaso que
conteniacutea 10 ml de la solucioacuten tamponada de F68 y se mantuvo bajo agitacioacuten magneacutetica durante
2 min El disolvente orgaacutenico se extrajo luego raacutepidamente por evaporacioacuten al vaciacuteo hasta un
volumen final de 8 ml
422 Limpieza y almacenamiento
Despueacutes de la evaporacioacuten del disolvente orgaacutenico la muestra se centrifugoacute durante 10 min a
20 deg C a 14000 o 12000 rpm para los meacutetodos O-F68 y W-F68 respectivamente El sobrenadante
se filtroacute usando filtros de 100 nm para medir la proteiacutena no encapsulada libre El sedimento se
resuspendioacute luego en PB hasta un volumen final de 4 ml y se mantuvo en refrigeracioacuten a 4ordmC
1La carga de proteiacutenas y la eficiencia de encapsulacioacuten
La carga de proteiacutena inicial se optimizoacute para la formulacioacuten de nanopartiacuteculas preservando la
estabilidad coloidal final despueacutes del paso de evaporacioacuten y siendo diferente para cada
nanosistema Ademaacutes se usoacute 16 p p (Lys PLGA) para O-F68 y 08 p p (Lys PLGA)
para W-F68 La cantidad de lisozima encapsulada se calculoacute midiendo la diferencia entre la
cantidad inicial antildeadida y la proteiacutena libre no encapsulada que se analizoacute mediante el ensayo con
62
aacutecido bicinconiacutenico (BCA Sigma-Aldrich) Luego la eficacia de encapsulacioacuten de proteiacutenas (EE)
y la carga de faacutermaco final (DL) se calcularon de la siguiente manera
EE = 119872119868minus119872119865
119872119868119909 100 119863119871 =
119872119868minus119872119865
119872119868119901119900119897119894119898119890119903 119909 10
donde MI es la masa total inicial de Lys MF es la masa total de Lys en el sobrenadante acuoso
y Mpoliacutemero es la masa de PLGA en la formulacioacuten
423 Caracterizacioacuten de las nanoparticulas
2Caracterizacioacuten interfacial de la primera emulsioacuten agua en aceite
La tensioacuten superficial y las mediciones de reologiacutea dilatacional en la interfaz aire-agua se
realizaron en el OCTOPUS (Maldonado-Valderrama et al 2013) dispositivo de anaacutelisis de
superficies por gota pendiente con intercambio muacuteltiple de subfase (patente presentada
P201001588) descrito en detalle por Cabrerizo-Viacutelchez y col (Wege Holgado-Terriza and
Cabrerizo-Vilchez 2002) Aquiacute el aire juega el papel de la fase orgaacutenica La tensioacuten superficial
se calcula con el software DINATENreg basado en el anaacutelisis de forma de gota axisimeacutetrica
(ADSA) y el moacutedulo de dilatacioacuten (E) de la capa interfacial se determina a partir del anaacutelisis de
imagen con el programa CONTACTOreg
3Morfologiacutea de la partiacutecula
Las nanopartiacuteculas se obtuvieron por microscopiacutea electroacutenica de barrido (SEM) y microscopiacutea
electroacutenica de transmisioacuten de barrido (STEM) usando un microscopio electroacutenico de barrido de
emisioacuten de campo Zeiss SUPRA 40VP del Centro de Instrumentacioacuten Cientiacutefica de la Universidad
de Granada (CIC UGR)
4Tamantildeo de las nanoparticulas y movilidad electrocineacutetica
El diaacutemetro hidrodinaacutemico y la movilidad electroforeacutetica de las NP se determinaron usando un
dispositivo Zetasizer NanoZeta ZS (Malvern Instrument Ltd UK) que trabaja a 25 deg C con un
63
laacuteser He-Ne de 633 nm y un aacutengulo de dispersioacuten de 173 deg Cada punto de datos se tomoacute como un
promedio de tres mediciones de muestra independientes El tamantildeo de las NP se caracterizoacute por
escaacutener de luz dinaacutemica (DLS) Se calcularon el diaacutemetro hidrodinaacutemico promedio (media Z o
media acumulada) y el iacutendice de polidispersidad (PDI) Estos paraacutemetros se calculan a traveacutes de
un anaacutelisis acumulativo de los datos que es aplicable para las distribuciones de tamantildeo
monomodal estrecho (Hassan Rana and Verma 2015) Tambieacuten determinamos la distribucioacuten
del tamantildeo de intensidad a partir de un algoritmo proporcionado por el software Zetasizer
La movilidad electroforeacutetica se determinoacute mediante la teacutecnica de electroforesis laacuteser Doppler
Se establecioacute una distribucioacuten de movilidad electroforeacutetica asiacute como una movilidad
electroforeacutetica promedio para cada muestra (entendiendo por promedio dos veces seguidas para
cada una de las muestras)
La distribucioacuten del tamantildeo hidrodinaacutemico de las NP con distribuciones de tamantildeo amplio de
DLS tambieacuten se midioacute usando anaacutelisis de seguimiento de nanopartiacuteculas (NTA) en un NanoSight
LM10-HS (GB) FT14 (NanoSight Amesbury Reino Unido) Todas las muestras se midieron maacutes
de tres veces durante 60 s con ajuste manual del obturador ganancia brillo y umbral a 25 deg C La
distribucioacuten de tamantildeo promedio (concentracioacuten de partiacuteculas frente a diaacutemetro) se calculoacute como
un promedio de al menos tres independientes distribuciones del tamantildeo
5Resonancia magneacutetica nuclear (RMN) de las nanopartiacuteculas
El espectro de 1HNMR de F68 libre las partiacuteculas cargadas con lisozima del meacutetodo O-F68
con y sin F68 y las partiacuteculas cargadas con lisozima del meacutetodo W-F68 se midieron con un
espectroacutemetro VNMRS de 500 MHz (Agilent) en el Centro de Instrumentacioacuten Cientiacutefica (CIC)
de la Universidad de Granada
424 Estabilidad coloidal y temporal en biologiacutea media
Se midioacute el diaacutemetro hidrodinaacutemico promedio y el iacutendice de polidispersidad (PDI) por DLS de
cada sistema para determinar su estabilidad coloidal en diferentes medios (tampoacuten de fosfato
64
[PB] solucioacuten salina tamponada con fosfato [PBS] y medio de cultivo celular medio de Eagle
modificado de Dulbecco [DMEM] de Sigma) y en diferentes momentos despueacutes (0 1 y 5 diacuteas)
Los experimentos de liberacioacuten in vitro se realizaron siguiendo una metodologiacutea similar a la
descrita anteriormente (eficacia de encapsulacioacuten) pero utilizando 1 ml de cada muestra
suspendida en PBS a 37 C La proteiacutena liberada de estas muestras se determinoacute cada 24 horas
mediante anaacutelisis de sobrenadante y el sedimento se suspendioacute en el mismo volumen de tampoacuten
para mantener las condiciones de liberacioacuten Todos los experimentos fueron desarrollados por
triplicado
6Microscopia confocal
La lisozima se marcoacute con isotopo de fluoresceiacutena (FITC) usando un meacutetodo descrito por Kok
et al (Kok et al 1998) Despueacutes de la conjugacioacuten covalente de FITC y lisozima las
concentraciones se estimaron espectrofotomeacutetricamente utilizando los coeficientes de extincioacuten
descritos para FITC a 494 nm y 280 nm La concentracioacuten de lisozima se calculoacute midiendo la
absorbancia oacuteptica a 280 nm y restando la absorbancia FITC correspondiente a esta longitud de
onda Las imaacutegenes se realizaron en un microscopio confocal de escaneo laacuteser Nikon A1 de CIC
UGR Todos los experimentos se realizaron por triplicado y se replicaron al menos dos veces
425 Actividad bioloacutegica e interacciones
7Actividad bioloacutegica de la lisozima
La actividad bioloacutegica de la lisozima se analizoacute mediante un kit de actividad enzimaacutetica
(Sigma-Aldrich) utilizando ceacutelulas de Micrococcus lysodeikticus como sustrato siguiendo las
instrucciones del fabricante
8Captacioacuten celular
Se tomaron ceacutelulas madre mesenquimales humanas primarias (hMSC) del hueso alveolar
maxilar sano de acuerdo con protocolos descritos (Mason et al 2014) Despueacutes de confirmar su
fenotipo por pruebas de citometriacutea de flujo y diferenciacioacuten trilinaje 12000 ceacutelulas por pozo se
cultivaron en placas esteacuteriles con fondo de vidrio (Ibidi cat n 81158) durante la noche Estas
65
ceacutelulas fueron tratadas con medio sin suero fetal bovino (FBS) y guiacutea celular roja (1 5000)
(C34552 ThermoFisher) durante 30 min Entonces el medio fue eliminado y complementado con
10 de SFB despueacutes de lo cual se agregaron partiacuteculas con lisozima-FITC Entonces los hMSCs
eran incubadas 30 minutos nuevamente lavadas tres veces con PBS 1X y un suplementado medio
fresco con 2 de FBS agregado Finalmente las hMSCs fueron examinadas por un microscopio
confocal (Nikon Eclipse Ti-E) Cultivo celular en todos los casos se mantuvieron a 37 deg C y 5
de atmoacutesfera de CO
43 Resultados y discusioacuten
431 Formulacioacuten de las nanoparticulas
Los meacutetodos desarrollados en este trabajo estaacuten destinados a mejorar las teacutecnicas de
formulacioacuten existentes para las NP de PLGA cargadas de proteiacutenas hidrofiacutelicas basadas en un
proceso de doble emulsioacuten (Blanco and Alonso 1998) (Csaba et al 2004) La novedad de estos
meacutetodos es el uso del surfactante polimeacuterico F68 ya sea en la fase orgaacutenica (meacutetodo O-F68) o en
la fase acuosa (W-F68) Este surfactante reduce el tamantildeo de las NP mejora su estabilidad y
protege la proteiacutena encapsulada Ademaacutes la presencia de F68 en la superficie de las partiacuteculas
reduce el reconocimiento de los nanovehiacuteculos (nanotransportadores) por el sistema mononuclear
fagociacutetico (MPS) (Farace et al 2016)
Ademaacutes la eleccioacuten del solvente orgaacutenico afecta significativamente las propiedades del
sistema coloidal final ya que la solubilidad del solvente orgaacutenico regula la estructura interna y
superficial de la partiacutecula Ademaacutes la interaccioacuten del disolvente con la biomoleacutecula encapsulada
puede alterar su bioactividad como consecuencia de su desnaturalizacioacuten como se encontroacute para
el cloruro de metileno (Meng et al 2003) En el meacutetodo O-F68 se elige DMC como disolvente
orgaacutenico debido a su menor solubilidad en agua para facilitar el proceso de emulsificacioacuten y su
bajo punto de ebullicioacuten para facilitar la evaporacioacuten Sin embargo se antildeadioacute un solvente orgaacutenico
libremente miscible en agua (acetona) y el emulsionante F68 en esta fase orgaacutenica para reducir
66
sus efectos bioloacutegicos negativos sobre la proteiacutena encapsulada (Danhier et al 2012) Este
emulsionante tambieacuten reduce la interaccioacuten de la matriz de PLGA proteiacutena-hidrofoacutebica y por lo
tanto la interrupcioacuten de la estructura de la proteiacutena (Ortega-Oller et al 2015) Por el contrario
en el meacutetodo W-F68 se utilizoacute acetato de etilo como disolvente orgaacutenico que ejerce menos
efectos de desnaturalizacioacuten sobre la proteiacutena encapsulada (Sturesson and Carlfors 2000) La
mayor solubilidad en agua de este solvente favorece la eliminacioacuten raacutepida del solvente La
velocidad de eliminacioacuten del disolvente tambieacuten se acelera al aumentar la tensioacuten de cizallamiento
durante la segunda etapa de emulsificacioacuten Tambieacuten mejora la eficacia del encapsulamiento y
minimiza el tiempo de contacto entre la proteiacutena y el disolvente orgaacutenico (Ortega-Oller et al
2015) El poloxaacutemero F68 se introduce en la fase acuosa externa
Ambas formulaciones (O-F68 y W-F68) (Tabla 2) dieron lugar a muestras coloidalmente
estables y a la encapsulacioacuten de la lisozima dentro de las nanopartiacuteculas de acuerdo con el doble
meacutetodo de emulsioacuten WOW (Makadia and Siegel 2011)
67
PLGA
(mg)
F68
(mg)
LYSI
(mg)
Initial EE
LYSF
(mg)
DL
O-F68-Lys 25 15 04 16 625 025 1
W-F68-Lys 100 2 08 08 731 058 058
Tabla 2 Condiciones de formulacioacuten y resultados de encapsulacioacuten de proteiacutenas PLGA F68
y LYSI son la cantidad inicial de poliacutemero surfactante y lisozima respectivamente El inicial
es la tasa inicial de proteiacutena-poliacutemero en pesopeso EE es la eficiencia de encapsulacioacuten LYSF
es la cantidad final encapsulada de lisozima DL es la tasa final de carga del faacutermaco en
pesopeso
La lisozima fue elegida como una proteiacutena modelo debido a su bioestabilidad sus
caracteriacutesticas bien conocidas y su facilidad para cuantificar su actividad bioloacutegica (Lin et al
2007) (Cai et al 2008) Ademaacutes su tamantildeo molecular (143 kD) y su punto isoeleacutectrico baacutesico
(alrededor de pH = 11) lo convierten en un modelo apropiado para otras proteiacutenas como los
factores de crecimiento oacuteseo (White et al 2013) Tres objetivos principales impulsaron la
optimizacioacuten de la relacioacuten apropiada entre el poliacutemero el poloxaacutemero y la proteiacutena (1) para
tener nanosistemas coloides estables de tamantildeos submicromeacutetricos (2) encapsular una cantidad
suficiente de proteiacutena y (3) para prevenir la desestabilizacioacuten de proteiacutenas manteniendo su
actividad bioloacutegica
Por lo tanto independientemente del meacutetodo de formulacioacuten se pretendiacutea limitar la carga de
proteiacutena inicial para proporcionar nanosistemas estables coloidalmente En nuestro caso como se
muestra en la Tabla 2 los valores de iniciales fueron la mejor opcioacuten para mantener la
estabilidad coloidal sin cambiar significativamente la distribucioacuten del tamantildeo (ver a
continuacioacuten) En consecuencia DL presenta valores relativamente bajos para ambas
formulaciones aunque la cantidad encapsulada de lisozima LYSF es mayor que las requeridas
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para proteiacutenas terapeacuteuticas con cantidades cliacutenicamente efectivas maacutes bajas (Paillard-Giteau et
al 2010) El valor de EE encontrado para las NP de O-F68-Lys estaacute en consonancia con las
caracteriacutesticas de la formulacioacuten y es similar a otros informes con diferentes proteiacutenas (Manuel J
Santander-Ortega Csaba et al 2010) (Blanco and Alonso 1998) (Santander-Ortega et al 2009)
(drsquoAngelo et al 2010) incluida la albuacutemina de suero bovino (BSA) o la insulina (Manuel J
Santander-Ortega Csaba et al 2010) (Santander-Ortega et al 2009) y varios factores de
crecimiento (drsquoAngelo et al 2010)
La presencia de surfactante estabiliza la emulsioacuten y reduce su tamantildeo Sin embargo tambieacuten
altera la interaccioacuten proteiacutena-poliacutemero lo que se traduce en una reduccioacuten de la eficacia de
encapsulacioacuten Esto fue evidenciado por Blanco y colaboradores al encapsular BSA y lisozima en
diferentes micropartiacuteculas de PLGA-poloxaacutemero (Blanco and Alonso 1998) Ademaacutes el tipo de
proteiacutena y su carga teoacuterica inicial son factores directamente relacionados con la EE y pueden
afectar la estabilidad coloidal de la emulsioacuten primaria como lo muestran Santander y
colaboradores (Manuel J Santander-Ortega Csaba et al 2010) La diferente relacioacuten poliacutemero
tensioactivo entre las dos formulaciones no es comparable ya que el tensioactivo se agrega de una
manera diferente En ambos casos utilizamos formulaciones anteriores como punto de partida
(Blanco and Alonso 1998) (Csaba et al 2004) y probamos varias relaciones poliacutemero
tensioactivo (datos no mostrados) con el fin de obtener la mejor estabilidad coloidal EE y DL
En la Tabla 2 mostramos los datos para las relaciones optimizadas de PLGA F68 en ambos
sistemas
En el meacutetodo W-F68 a pesar del mayor valor de EE con respecto al sistema O-F68 se esperaba
una encapsulacioacuten casi completa debido a la baja relacioacuten inicial de proteiacutena masa de PLGA
(Manuel J Santander-Ortega Csaba et al 2010) y a la ausencia de surfactante en la primera
emulsioacuten Las caracteriacutesticas del proceso de formulacioacuten modificado pueden tener la clave En
esta formulacioacuten la solubilidad relativamente alta del acetato de etilo en agua promueve la
difusioacuten raacutepida del disolvente orgaacutenico en la segunda fase acuosa Inicialmente se agrega un
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pequentildeo volumen inicial de agua que contiene poloxaacutemero para evitar una precipitacioacuten raacutepida e
incontrolada del poliacutemero y para controlar la velocidad del proceso Esto se complementa
posteriormente con la adicioacuten de un volumen acuoso maacutes grande como se describioacute anteriormente
(Meng et al 2003) Cuando esta solidificacioacuten es lenta favorece el escape de la proteiacutena y la EE
disminuye Sin embargo si la solidificacioacuten es muy raacutepida el contacto de la proteiacutena con el
solvente orgaacutenico se minimiza y la EE aumenta En el lado negativo puede producir aglomeracioacuten
de poliacutemero que interfiere con la correcta formacioacuten de las NP La introduccioacuten de un paso
intermedio con un volumen reducido de fase acuosa con poloxaacutemero puede modular la velocidad
del proceso controlando la difusioacuten de acetato de etilo en el agua y permitiendo la difusioacuten en la
fase orgaacutenica del poloxaacutemero Una velocidad controlada del proceso de pre-solidificacioacuten del
poliacutemero en presencia de surfactante puede producir canales o poros en la cubierta polimeacuterica
que por un lado podriacutean facilitar la liberacioacuten de proteiacutena y por otro lado podriacutea reducir el valor
EE (Rosca Watari and Uo 2004) Como resultado de estos fenoacutemenos los DL finales (p p de la
lisozima poliacutemero) que se muestran en la Tabla 2 para ambos sistemas NP son adecuados para
su aplicacioacuten como sistemas de nanotransporte
432 Caracterizacioacuten de las Nanopartiacuteculas
9Caracterizacioacuten interfacial de la primera formulacioacuten de agua en aceite
Para obtener una mejor comprensioacuten del efecto del meacutetodo de formulacioacuten sobre las
propiedades interfaciales de la primera solucioacuten de agua (solucioacuten de lisozima) emulsioacuten en
aceite disentildeamos experimentos de superficie con lisozima y Pluronicreg F68 La diferencia
principal en los dos meacutetodos de formulacioacuten es coacutemo se agrega Pluronicreg F68 en fase acuosa
(W-F68) o en fase orgaacutenica (O-F68) Esta diferencia podriacutea afectar la composicioacuten de la superficie
de las NP y como resultado sus propiedades coloidales
La tensioacuten superficial y la elasticidad en el interfaz aire-agua fueron las propiedades analizadas
(Tabla 3) En esta interfaz las proteiacutenas cambian su conformacioacuten y exponen su parte hidrofoacutebica
al aire dependiendo de su estabilidad termodinaacutemica flexibilidad anfipaticidad tamantildeo
70
molecular y carga En nuestro caso la lisozima es una proteiacutena globular que se adsorbe en la
interfase aire-agua y forma una monocapa riacutegida debido a su estructura interna y la presencia y
cantidad de puentes disulfuro (Pezennec et al 2008) Nuestras mediciones se realizaron a pH 12
por lo tanto la lisozima estaacute cargada negativamente La Tabla 3 muestra la tensioacuten interfacial de
la monocapa de lisozima en la interfaz aire-agua despueacutes de 50 minutos de adsorcioacuten (457 plusmn 04
(mN m)) y su elasticidad (83 plusmn 4 (mN m)) La reduccioacuten de la tensioacuten interfacial en comparacioacuten
con la de la interfaz aire-agua (72 mN m) indica las caracteriacutesticas de tensioactivo de la lisozima
El alto valor de la elasticidad se debioacute a la carga y a las altas interacciones moleculares en la
monocapa de lisozima Cuando la monocapa se forma con Pluronicreg F68 la tensioacuten superficial
es ligeramente menor que con la lisozima cuando se agrega Pluronicreg en AP pero similar
(teniendo en cuenta el error) cuando se agrega en OP
Pluronicreg F68 es una moleacutecula desmontable hiacutebrida que se introduce en el interfaz aire-agua
cuando se disuelve en fase acuosa y tambieacuten cuando se deposita en la superficie de la partiacutecula
Se encuentran pequentildeas diferencias al comparar la tensioacuten superficial de la monocapa Pluronicreg
de los dos meacutetodos Los diferentes valores de tensioacuten interfacial alcanzados en ambos casos se
deben a los diferentes meacutetodos para agregar Pluronicreg F68 a la monocapa de lisozima formada
Pluronicreg F68 presenta una elasticidad menor que la lisozima como se esperaba ya que se sabe
que Pluronicreg F68 forma una monocapa flexible en la interfaz aire-agua (Torcello-Goacutemez et al
2011)
71
Primer Paso
Tensioacuten
Interfacial
(mNm)
Elasticidada
(mNm)
Segundo
Paso
Tensioacuten
Interfacial
(mNm)
Elasticidadb
(mNm)
Lisozima 457plusmn04 83plusmn4 - - -
Lisozima 457plusmn04 83plusmn4 - - -
Pluronicreg
F68 (AP) 421plusmn03 15plusmn3
Pluronicreg
F68 (AP) 379plusmn06 142plusmn05
Pluronicreg
F68 (OP) 475plusmn21 94plusmn05
Pluronicreg
F68 (OP) 38plusmn2 43plusmn4
Tabla 3 Tensioacuten interfacial y elasticidad dilatacional (a 1 Hz) de la interfase aire-agua (a)
despueacutes de adsorber lisozima o Pluronic F68 en la fase acuosa (AP) o Pluronicreg F68 en fase
orgaacutenica (OP) en el primer paso (b) cuando Pluronic F68 se agrega en AP u OP despueacutes de la
adsorcioacuten de la monocapa de lisozima (media plusmn sd n = 3)
Se disentildearon dos ensayos para imitar los meacutetodos de formulacioacuten de las partiacuteculas En el primer
ensayo (meacutetodo W-F68) se formoacute una monocapa de lisozima luego la mayor parte de la
partiacutecula se intercambioacute con la solucioacuten acuosa de Pluronicreg F68 y despueacutes de la adsorcioacuten se
midieron la tensioacuten interfacial y la elasticidad del interfaz (379 plusmn 06 mN my 142 plusmn 05 mN
m respectivamente) Este bajo valor de elasticidad fue muy similar al de la monocapa de
Pluronicreg F68 lo que indica que Pluronicreg F68 se encuentra en el interfaz adecuado y elimina
la lisozima previamente adsorbida En el segundo ensayo (meacutetodo O-F68) despueacutes de que se
formara la monocapa de lisozima Pluronicreg F68 disuelto en cloroformo se depositoacute sobre la
superficie de la partiacutecula El cloroformo se evapora raacutepidamente y se mide la tensioacuten interfacial y
la elasticidad del interfaz (38 plusmn 2 mN my 43 plusmn 4 mN m respectivamente) La elasticidad fue la
mitad de la de la monocapa de lisozima pura tal vez debido a la coexistencia de las moleacuteculas de
72
lisozima y Pluronicreg F68 en el interfaz La tensioacuten superficial del interfaz final no depende del
meacutetodo de adicioacuten de Pluronicreg pero es menor que la de la lisozima pura o que el Pluronicreg
puro
Dentro de este contexto se ha informado ampliamente que la adsorcioacuten de PEO y poloxaacutemeros
en el interfaz reduce la unioacuten a proteiacutenas (Manuel J Santander-Ortega Lozano-Loacutepez et al
2010) (Torcello-Goacutemez et al 2011) En el meacutetodo O-F68 la lisozima se expone al DCM despueacutes
de la formacioacuten de la primera emulsioacuten de agua en aceite incluso si se agrega Pluronicreg ya que
ambos coexisten en el interfaz En el meacutetodo W-F68 la proteiacutena estaraacute en contacto con el acetato
de etilo en este paso ya que el Pluronicreg estaacute ausente Sin embargo este disolvente tiene efectos
bioloacutegicos maacutes deacutebiles sobre la lisozima Pluronicreg podriacutea alcanzar el interfaz cuando se agrega
a la fase acuosa en el siguiente paso y desplazar la proteiacutena del interfaz que podriacutea difundirse
hacia afuera a la fase acuosa
10Morfologiacutea de la partiacutecula
La entrega la biodistribucioacuten y el mecanismo de accioacuten de un faacutermaco o biomoleacutecula
transportada dependen en gran medida del tamantildeo de la partiacutecula la concentracioacuten y el tiempo
(Penaloza et al 2017) En general la escala micromeacutetrica estaacute disentildeada para un suministro local
que permite la formacioacuten de reservorios de la moleacutecula transportada y minimiza la accioacuten del
sistema fagociacutetico (Schwendeman et al 2014) Sin embargo los sistemas nanomeacutetricos son maacutes
versaacutetiles porque permiten una distribucioacuten sisteacutemica son maacutes estables reactivos y permiten la
accioacuten extra e intracelular Este uacuteltimo mecanismo es esencial cuando la moleacutecula o el faacutermaco
debe actuar en el citoplasma (Wang et al 2012) o en cualquier otra estructura intracelular como
la mitocondria el aparato de Golgi el retiacuteculo endoplaacutesmico o el nuacutecleo (Penaloza et al 2017)
(Vasir and Labhasetwar 2007) (Yameen et al 2014) Tambieacuten se han investigado otros
paraacutemetros para alterar el destino intracelular de las partiacuteculas principalmente alterando la
decoracioacuten de su superficie (Sneh-Edri Likhtenshtein and Stepensky 2011) por ejemplo con
sentildeales de localizacioacuten nuclear (NLS) que utilizan el nuacutecleo como el objetivo de la partiacutecula (Vasir
73
and Labhasetwar 2007) Sin embargo estas estrategias auacuten se encuentran en fase de desarrollo
inicial (Penaloza et al 2017) (Yameen et al 2014)
Se buscoacute un tamantildeo de partiacutecula en la escala submicromeacutetrica (entre 2 y 500 nm) ya que es
necesario para la internalizacioacuten celular y una distribucioacuten raacutepida despueacutes de la administracioacuten
parenteral para alcanzar diferentes tejidos a traveacutes de diferentes barreras bioloacutegicas Las partiacuteculas
de menos de 200 nm minimizan su ingesta por macroacutefagos El tipo de disolvente orgaacutenico la
concentracioacuten del poliacutemero la adicioacuten de surfactante y la energiacutea de emulsioacuten controlan el tamantildeo
del sistema
El meacutetodo O-F68 da lugar a una distribucioacuten monomodal del tamantildeo de partiacuteculas con
diaacutemetros alrededor de 100 nm La adicioacuten de Pluronicreg F68 en la fase orgaacutenica refuerza la
estabilidad coloidal de la primera emulsioacuten y reduce el tamantildeo de partiacutecula en comparacioacuten con
las NP de PLGA en las que la estabilidad es puramente electrostaacutetica debido a los grupos
carboxiacutelicos del PLGA En el meacutetodo W-F68 se tienen en cuenta el esfuerzo cortante y el
volumen de la fase acuosa para producir un sistema con partiacuteculas de entre 100 y 500 nm
Las NP de O-F68-Lys tienen una forma esfeacuterica con una distribucioacuten de tamantildeo monomodal
(diaacutemetros alrededor de 100 nm) y una estructura nuacutecleo-capa (Fig 7a) Las partiacuteculas vaciacuteas
producidas con el meacutetodo O-F68 se muestran en las Figs 7 (sin F68) y Fig 8 (con F68) Tambieacuten
son esfeacutericas y con una estructura nuacutecleo-caparazoacuten pero un poco maacutes grande
W-F68-Lys NP tambieacuten presenta una forma esfeacuterica pero una distribucioacuten de tamantildeo
multimodal con diaacutemetros entre 140 y 450 nm la poblacioacuten maacutes grande estaacute alrededor de 260 nm
(Fig 7b) Tambieacuten se observa una estructura nuacutecleo-capa en estas partiacuteculas Las partiacuteculas vaciacuteas
del meacutetodo W-F68 se presentan en la Fig 9 correspondiente a un sistema maacutes polidisperso
74
(a) (b)
(c)
Figura 7 Morfologiacutea de las nanopartiacuteculas Micrografiacuteas de SEM (a b) y STEM (c) de
nanopartiacuteculas vaciacuteas utilizando el meacutetodo O-F68 sin F68
75
(a) (b)
(c) (d)
Figura 8 Micrografias de SEM (a b) y STEM (c d) de partiacuteculas vaciacuteas usando el meacutetodo O-
F68 con F68
76
(a) (b)
Figura 9 Micrografiacuteas de SEM de partiacuteculas vacias usando el meacutetodo W-F68
11Tamantildeo de las nanopartiacuteculas movilidad electrocineacutetica y estabilidad coloidal
La distribucioacuten del diaacutemetro hidrodinaacutemico de las partiacuteculas se determinoacute en primer lugar por
DLS La Tabla 4 contiene las principales propiedades coloidales de las partiacuteculas producidas con
los meacutetodos O-F68 y W-F68 vaciacuteas o cargadas con lisozima Los resultados de partiacuteculas vaciacuteas
del meacutetodo O-F68 pero sintetizados sin F68 tambieacuten estaacuten incluidos
Los paraacutemetros de tamantildeo se calcularon a traveacutes de un anaacutelisis acumulativo de los datos que
es aplicable para distribuciones de tamantildeo monomodal estrechas (Hassan Rana and Verma
2015) Las micrografiacuteas SEM y STEM indican que se podriacutea suponer tal aproximacioacuten para las
partiacuteculas del meacutetodo O-F68 pero no del W-F68 Por lo tanto las distribuciones de tamantildeo de
intensidad de los diferentes sistemas se muestran en la figura 11a La presencia de Pluronicreg F68
en el meacutetodo O-F68 reduce significativamente el tamantildeo y la polidispersidad de las NP Esto
concuerda con la reduccioacuten de la tensioacuten superficial cuando el F68 estaacute en el interfaz (Tabla 3)
lo que promueve el proceso de emulsioacuten Si las NP tambieacuten estaacuten cargadas con lisozima el tamantildeo
es auacuten menor pero la polidispersidad aumenta ligeramente en comparacioacuten con las partiacuteculas
vaciacuteas Las propiedades surfactantes de la lisozima se han demostrado con los resultados de la
tensioacuten superficial (Tabla 3)
La Fig 11a indica la presencia de partiacuteculas superiores a 500 nm con el W-F68 que no se
correlaciona con las micrografiacuteas SEM Por lo tanto se utilizoacute una teacutecnica diferente (NTA) para
77
obtener informacioacuten sobre la distribucioacuten del tamantildeo de dichos sistemas (figura 12b) Con NTA
la distribucioacuten de tamantildeos fue consistente con las imaacutegenes SEM Se encontraron distribuciones
de gran tamantildeo correspondientes a los sistemas multimodales con este meacutetodo pero la adicioacuten de
lisozima condujo a una clara reduccioacuten del tamantildeo Esto se debe a que la lisozima tambieacuten actuacutea
como emulsionante en la primera emulsioacuten
La carga electrocineacutetica de las NP se analizoacute midiendo la movilidad electroforeacutetica Para
comparacioacuten todas las muestras se midieron a pH 7 (tampoacuten de fosfato) En la Fig 12 se
presentan las distribuciones de movilidad electroforeacutetica mientras que los promedios
correspondientes se muestran en la Tabla 4
Las NP de PLGA normalmente estaacuten cargadas de forma negativa debido a los grupos
carboxiacutelicos del poliacutemero El uso de Pluronicreg F68 en el meacutetodo O-F68 reduce claramente la
movilidad electroforeacutetica de las NP lo que indica que algo de Pluronicreg estaacute ubicado en la
superficie de las NP Esta reduccioacuten se esperaba despueacutes de la incorporacioacuten de este tensioactivo
no ioacutenico al interfaz ya que la presencia de cadenas de oacutexido de polietileno causariacutea un
desplazamiento hacia afuera del plano de corte donde se define el potencial y esto disminuiriacutea
posteriormente la movilidad electroforeacutetica Los resultados previos para partiacuteculas de PLGA han
mostrado una reduccioacuten significativa directamente relacionada con el recubrimiento de
poloxaacutemero (Santander-Ortega et al 2006) Si comparamos los dos sistemas la superficie menos
negativa para las NP OF68 se relacionariacutea con una menor densidad del poliacutemero PLGA de
superficie llevando la carga eleacutectrica negativa a el interfaz Este resultado estariacutea en liacutenea con la
mayor cantidad de PLGA en la formulacioacuten del nanosistema WF68
78
Figura 10 Micrografiacuteas SEM y STEM de partiacuteculas cargadas de lisozima usando el meacutetodo
O-F68 (a) o W-F68 (b)
79
Media Z (nm) PDI Media-μ (micromcmVs)
Meacutetodo
O-F68
Vaciacuteo sin F68 266 plusmn 7 0293 -506 plusmn 015
Vaciacuteo 1627 plusmn 21 0081 -429 plusmn 018
Cargada de
Lisozima 1210 plusmn12 0244 -334 plusmn 007
Meacutetodo
W-F68
Vaciacuteo 273 plusmn 3 0193 -531 plusmn 011
Cargada de
Lisozima 293 plusmn 4 0169 -4212 plusmn 0013
Tabla 4 Propiedades coloidales de PLGA NP de diferentes meacutetodos de formulacioacuten Se
midieron en tampoacuten de fosfato (pH 7) El diaacutemetro hidrodinaacutemico promedio (media Z o media
acumulada) y el iacutendice de polidispersidad (PDI) se determinan a partir de DLS (Media plusmn sd n
= 3)
80
(a)
(b)
Figura 11 Distribucioacuten del diaacutemetro hidrodinaacutemico (a) mediante DLS a pH 7 (tampoacuten de
fosfato) de partiacuteculas de PLGA vaciacuteas y cargadas con lisozima de los meacutetodos O-F68 y W-F68
y (b) mediante NTA a pH 7 (tampoacuten de fosfato) de partiacuteculas de PLGA vaciacuteas y cargadas con
lisozima del meacutetodo W-F68
81
(a)
(b)
Figura 12 Distribucioacuten de movilidad electroforeacutetica a pH 7 (tampoacuten de fosfato) de partiacuteculas
de PLGA cargadas con lisozima y vaciacuteas a partir de los meacutetodos (a) O-F68 y (b) W-F68
82
Cuando la lisozima tambieacuten se utiliza en la siacutentesis la superficie es auacuten menos negativa lo que
podriacutea explicarse por la presencia de alguna proteiacutena (cuya carga neta es positiva) cerca o en el
interfaz Este uacuteltimo efecto tambieacuten se encuentra con el meacutetodo W-F68 La atractiva interaccioacuten
electrostaacutetica entre los residuos de aacutecido terminales negativos de PLGA y las moleacuteculas de
lisozima juega un papel clave en el proceso de encapsulacioacuten de proteiacutenas (Paillard-Giteau et al
2010) o adsorcioacuten (Cai et al 2008) en PLGA NPs que afecta la carga final de proteiacutenas
En relacioacuten con esta situacioacuten una caracteriacutestica importante de la formulacioacuten de
encapsulacioacuten W-F68 es que la fase acuosa tiene un pH de 12 lo que permite una carga neta
negativa de lisozima y por lo tanto evita la atraccioacuten electrostaacutetica de la proteiacutena y el poliacutemero
Esta situacioacuten puede reducir la eficacia de la encapsulacioacuten pero al mismo tiempo favorece el
posterior proceso de difusioacuten de proteiacutenas y en consecuencia la liberacioacuten a corto plazo
Estudios recientes han propuesto el uso de nanopartiacuteculas incrustadas en scaffolds impresos en
3D predisentildeados (Baumann et al 2017) (Lee et al 2017) lo que nos lleva a analizar la
estabilidad de las dos formulaciones en varios medios generalmente empleados durante la
preparacioacuten de otras estructuras Se encontraron distribuciones de tamantildeo similares al original
para las dos formulaciones en diferentes medios (PB PBS y DMEM) y en diferentes momentos
despueacutes de la siacutentesis (0 1 y 5 diacuteas) La carga eleacutectrica de los grupos terminales de aacutecido PLGA
y las moleacuteculas de poloxaacutemero ubicadas en la superficie NP confiere un mecanismo de estabilidad
coloidal combinado electrostaacutetico y esteacuterico como se ha descrito previamente (Manuel J
Santander-Ortega Lozano-Loacutepez et al 2010) (Santander-Ortega et al 2006) Ademaacutes las NPs
en todos los casos mantienen su tamantildeo almacenado a 4 deg C al menos durante 1 mes (datos no
mostrados) Por lo tanto los medios descritos podriacutean usarse potencialmente como medios de
almacenamiento o para preparar otras soluciones o scaffolds antes de colocarlos realmente en el
entorno vivo (in vitro o in vivo)
83
12NMR de las nanopartiacuteculas
En la Fig 12 tanto las NP vaciacuteas como las cargadas de proteiacutenas presentan una movilidad
electroforeacutetica menos negativa que las NP vaciacuteas sin F68 lo que podriacutea explicarse por la presencia
de Pluronicreg F68 en la superficie de la NP Al comparar los espectros 1HNMR de Pluronicreg F68
libre y las NP cargadas con lisozima de los meacutetodos O-F68 y W-F68 podemos verificar la
presencia de F68 en la superficie de las NP (figura 13) por los picos que se muestran entre 325 y
375 ppm y a 1 ppm Estos picos tambieacuten son visibles en los espectros de NP formulados con F68
(O-F68 y W-F68 figuras 14 y 15 respectivamente)
RMN de las nanopartiacuteculas
Figura 13 Espectro 1HMNR de F68 libre
84
Figura 14 Espectro de 1HMNR de partiacuteculas cargadas con lisozima del meacutetodo O-F68
Figura 15 Espectro de 1HMNR de partiacuteculas cargadas con lisozima del meacutetodo W-F68
433 Actividad bioloacutegica e interacciones
F68
F68
F68
F68
85
Una liberacioacuten controlada desde un sistema de administracioacuten basado en PLGA es una tarea
difiacutecil ya que depende de muacuteltiples factores el tipo de PLGA el solvente el estreacutes mecaacutenico el
uso de surfactantes etc (Hines and Kaplan 2013) La difusioacuten de la proteiacutena y la erosioacuten del
poliacutemero son los principales mecanismos implicados en la liberacioacuten de proteiacutena en los sistemas
de administracioacuten basados en PLGA Ademaacutes es tiacutepico encontrar una liberacioacuten en forma de una
raacutepida raacutefaga en la etapa inicial seguida de una fase de liberacioacuten lenta en un corto y mediano
plazo En esta fase las moleacuteculas de proteiacutena se difunden a traveacutes de la matriz del poliacutemero hasta
alcanzar una fase final en la que la degradacioacuten del poliacutemero por hidroacutelisis permite una liberacioacuten
maacutes raacutepida (Fredenberg et al 2011)
Por otro lado la liberacioacuten a corto plazo es de especial intereacutes para el transporte de factores de
crecimiento morfogeneacuteticos oacuteseos (BMP) Una explosioacuten inicial controlada seguida de una
liberacioacuten sostenida mejora significativamente la regeneracioacuten in vivo de hueso (Ortega-Oller et
al 2015) y cartiacutelago (Begam et al 2017) incluso en sistemas de liberacioacuten controlada doble
(Kim and Tabata 2015) Por estas razones centramos nuestro anaacutelisis en la liberacioacuten a corto
plazo teniendo en cuenta la reduccioacuten de la degradacioacuten del poliacutemero por hidroacutelisis encontrada
en sistemas similares para estos primeros pasos (Rescignano et al 2016)
86
Figura 16 Liberacioacuten acumulada (siacutembolos rellenos) y bioactividad residual (siacutembolos
abiertos) de O-F68-Lys (cuadrado) y W-F68-Lys (triaacutengulo) incubados durante diferentes
tiempos a 37 deg C en tampoacuten de fosfato salino (pH 74) (media plusmn sd n = 3)
La Fig 16 muestra la liberacioacuten acumulativa de lisozima de las NP de O-F68-Lys a corto plazo
(siete diacuteas) Estos resultados son consistentes con un proceso de dos pasos un estallido inicial y
uno de liberacioacuten lenta El primer paso podriacutea corresponder a la liberacioacuten de las moleacuteculas de
proteiacutena ubicadas cerca de la superficie cuya presencia se dedujo de los resultados de movilidad
electroforeacutetica (Fig 12) La segunda parte del proceso de liberacioacuten fue limitada y lenta debido a
la difusioacuten de proteiacutenas a traveacutes de la matriz de la cubierta polimeacuterica La interaccioacuten electrostaacutetica
especiacutefica entre las moleacuteculas de lisozima positiva y los grupos dxe aacutecido terminal negativo de
PLGA puede reducir la difusioacuten de proteiacutenas (Blanco and Alonso 1998) Cuando se agrega el
poloxaacutemero (F68) la interaccioacuten entre el surfactante y la proteiacutena ayuda al proceso de difusioacuten
lo que lleva a una liberacioacuten maacutes completa y sostenida (Manuel J Santander-Ortega Csaba et
87
al 2010) Tambieacuten ayuda a mantener la actividad bioloacutegica de la proteiacutena (Paillard-Giteau et al
2010) (Morille et al 2013) El poloxaacutemero reduce las interacciones proteiacutena-poliacutemero no
especiacuteficas (es decir interacciones hidroacutefobas) pero no las especiacuteficas (electrostaacutetica) por lo
tanto la difusioacuten a traveacutes de los poros llenos de agua o a traveacutes del poliacutemero sigue siendo limitada
En el estudio actual la fraccioacuten de proteiacutena liberada y el patroacuten de liberacioacuten son similares a los
encontrados en la literatura para la lisozima encapsulada en nano y micropartiacuteculas de mezclas de
PLGA y otros poliacutemeros o surfactantes (Meng et al 2003) (White et al 2013) (Perez De Jesus
and Griebenow 2002)
La curva de liberacioacuten de proteiacutenas de W-F68-Lys NP (Fig 16) revela que la tasa de
administracioacuten inicial es ideacutentica a la del sistema O-F68 lo que podriacutea significar una proporcioacuten
similar de proteiacutena encapsulada cerca o en la superficie para ambos sistemas NP Esto estariacutea de
acuerdo con la disminucioacuten anaacuteloga en la movilidad electroforeacutetica de las NP cargadas con
lisozima de la que informaron previamente (Fig 12) En la segunda parte del proceso la
interaccioacuten especiacutefica entre la proteiacutena y el poliacutemero estaacute nuevamente presente Sin embargo el
proceso de difusioacuten en el sistema W-F68 parece mejorar permitiendo una liberacioacuten continua y
sostenida despueacutes del estallido inicial y alcanzando un valor ligeramente maacutes alto para el tiempo
de liberacioacuten maacuteximo estudiado Este resultado podriacutea estar relacionado con la estructura interna
de la capa de poliacutemero que permite una mejor hidratacioacuten y por lo tanto una mejor difusioacuten de
la proteiacutena hacia el exterior Se ha informado previamente que el uso de disolventes orgaacutenicos
menos polares tales como DCM para formulaciones de partiacuteculas de PLGA aumenta la densidad
de la matriz de poliacutemero en comparacioacuten con disolventes orgaacutenicos maacutes polares tales como EA
Las matrices PLGA resultan maacutes resistentes en el primer caso pero reducen al mismo tiempo su
conectividad y difusioacuten (Bohr et al 2015) Meng y colaboradores (Meng et al 2003) encontraron
que una eliminacioacuten maacutes raacutepida de EA da como resultado una liberacioacuten cineacutetica maacutes lenta de la
proteiacutena debido a una disminucioacuten en la porosidad de las NP En cuanto al papel de Pluronicreg
Rafati y colaboradores (Rafati et al 2012) encontraron una mayor concentracioacuten de proteiacutena
88
encapsulada en los poros superficiales en micropartiacuteculas sintetizadas en presencia de surfactante
en la segunda fase acuosa de la emulsioacuten Dado que se introdujo un paso intermedio en nuestra
formulacioacuten W-F68 en la segunda fase acuosa de la emulsioacuten la eliminacioacuten de la EA por difusioacuten
se controloacute fuertemente de modo que se esperaba que la porosidad de estas NP aumentara Esta
porosidad mejora la difusioacuten de proteiacutenas lo que permite un patroacuten de liberacioacuten maacutes estable de
acuerdo con el resultado experimental encontrado para este sistema A pesar del efecto
desfavorable de la interaccioacuten proteiacutena-poliacutemero electrostaacutetico especiacutefico en la liberacioacuten la
cantidad de proteiacutena liberada en nuestras NP es sustancial lo que significa que hay otras
interacciones inespeciacuteficas que pueden ser moduladas por la presencia de surfactante permitiendo
un lanzamiento sostenido La cantidad de lisozima liberada es similar a la encontrada con la
lisozima fiacutesicamente adsorbida en la superficie de las nanopartiacuteculas de PLGA a pesar de la
atraccioacuten electrostaacutetica (Cai et al 2008) Ademaacutes de otras interacciones inespeciacuteficas la
concentracioacuten de electrolitos en el medio de liberacioacuten podriacutea modular esta atraccioacuten
electrostaacutetica entre la proteiacutena y el poliacutemero disminuyeacutendola y facilitando el proceso de
liberacioacuten (Manuel J Santander-Ortega Lozano-Loacutepez et al 2010)
Otro paraacutemetro notable es la actividad bioloacutegica de la liberacioacuten in vitro de lisozima que se
muestra en la Fig 16 Mientras que en el sistema O-F68 la bioactividad se reduce parcialmente
hasta en un 40 la proteiacutena suministrada por el sistema W-F68 mantiene la actividad por encima
del 90 con respecto a la de la lisozima suministrada comercialmente y se resuspende en el mismo
tampoacuten de liberacioacuten Como se discutioacute anteriormente tanto el solvente orgaacutenico como la
interaccioacuten hidrofoacutebica entre la proteiacutena y el poliacutemero a menudo causan la desnaturalizacioacuten de
las proteiacutenas encapsuladas (Paillard-Giteau et al 2010) (Gaudana et al 2013) Perez y
colaboradores (Perez De Jesus and Griebenow 2002) describen una peacuterdida parcial de actividad
cuando se usa DCM y una solucioacuten acuosa de PVA en la segunda etapa de emulsioacuten sin ninguacuten
excipiente adicional El uso de poloxaacutemeros en la formulacioacuten reduce tales interacciones mejora
la estabilidad de la proteiacutena y mantiene una capa acuosa que retiene las moleacuteculas de agua
89
necesarias para la funcioacuten bioloacutegica de la proteiacutena al mismo tiempo que ayuda a su difusioacuten Esta
situacioacuten junto con el uso de un solvente orgaacutenico deacutebil como EA ayuda a preservar la actividad
bioloacutegica de la lisozima como se encuentra para el sistema W-F68-Lys
La Fig 17 presenta diferentes imaacutegenes de microscopiacutea confocal relacionadas con el proceso
de liberacioacuten de NPs W-F68 cargadas de lisozima Una disminucioacuten en la intensidad de
fluorescencia fue apreciable a lo largo del experimento in vitro Ademaacutes la agregacioacuten del sistema
es visible a medida que avanza el proceso de incubacioacuten El anaacutelisis de estas imaacutegenes es
consistente con los resultados informados previamente para este sistema NP
90
Figura 17 Actividad bioloacutegica e interacciones Imaacutegenes octogonales obtenidas por
microscopiacutea confocal de Lisozima-FITC encapsulado en NPs W-F68 tras su incubacioacuten a
diferentes tiempos en tampon fosfato salino (pH 74) a 37ordmC Las NP se centrifugaron previamente
para eliminar la proteiacutena marcada liberada (a) Experimentos previos al lanzamiento (b)
despueacutes de 24h (c) despueacutes de 72h (d) despueacutes de 168h
13Captacioacuten celular
La captacioacuten celular de NP de PLGA es un proceso conocido que se ve afectado principalmente
por las propiedades de superficie la funcionalizacioacuten (Loureiro et al 2016) y la agregacioacuten de
partiacuteculas (Xiong et al 2011) La internalizacioacuten y el procesamiento intracelular posterior de las
partiacuteculas se ha descrito como un proceso activo por lo tanto depende de la energiacutea y puede
verse afectado por otros factores que alteran la absorcioacuten de energiacutea por parte de las ceacutelulas como
la temperatura (Penaloza et al 2017) Las partiacuteculas pueden internalizarse mediante varios
meacutetodos de endocitosis que dependen principalmente del tamantildeo de la partiacutecula partiacuteculas
(a) (b)
(c) (d)
91
dependientes de caveolina (diaacutemetro asymp 60 nm) independientes de clatrina (diaacutemetro asymp 90 nm) y
dependientes de clatrina (diaacutemetro asymp 120 nm) (Vasir and Labhasetwar 2007) (Yameen et al
2014) Una vez internalizados alrededor del 65 se exportan al espacio extracelular antes de
liberar cualquiera de sus contenidos mientras que el resto libera lentamente la moleacutecula
encapsulada en el espacio intracelular (Panyam and Labhasetwar 2003)
Figura 18 Proyeccioacuten z de 5 imaacutegenes de hMSCs visualizadas 30 minutos despueacutes de la
incubacioacuten con W-F68-LysFITCNPsorO-F68-LysFITCNPs Las hMSC se marcaron previamente
con Guiacutea celular roja Barra de escala 20 m
El proceso de liberacioacuten intracelular se ve afectado por la formulacioacuten de las partiacuteculas
(Penaloza et al 2017) Hemos demostrado que los sistemas propuestos siguen un patroacuten similar
a otros publicados anteriormente En un periodo de tiempo tan corto como 30 minutos despueacutes
de la incubacioacuten las NPs de W-F68-LysFITC fueron absorbidas por las ceacutelulas (Fig 18) Algunas
partiacuteculas W-F68 todaviacutea estaban en el medio por lo que la actividad dual podriacutea ocurrir Por el
92
contrario las NP de O-F68-LysFITC se vieron afectadas por la agregacioacuten y por lo tanto no
alcanzaron adecuadamente el espacio intracelular (Fig 18 para las imaacutegenes del eje Z veacutease la
Fig 19)
Superposicioacuten Canal Verde Canal Rojo P
royec
cioacuten z
93
Superposicioacuten Canal Verde Canal Rojo
Pro
yec
cioacuten z
94
Figura 19 Proyeccioacuten z de 5 imaacutegenes e imaacutegenes z independientes de hMSC visualizadas 30
minutos despueacutes de la incubacioacuten sin partiacuteculas y con NP W-F68-LysFITC o NP O-F68-LysFITC
Las hMSC se marcaron previamente con un rastreador celular rojo Barra de escala 20 microm
Pro
yec
cioacuten z
Superposicioacuten Canal Verde Canal Rojo
95
Esto contradice los anaacutelisis previos de la estabilidad coloidal en PB PBS y DMEM Este
hallazgo puede explicarse por el hecho de que aunque el medio de cultivo fue DMEM este uacuteltimo
medio se complementoacute con suero fetal bovino y las ceacutelulas liberan muchos factores al medio
extracelular que pueden afectar a este tipo de partiacuteculas Ninguno de los sistemas demostroacute ser
toacutexico para las ceacutelulas (Fig 20) No hay estudios disponibles que hayan informado alguacuten efecto
de la lisozima sobre las hMSC
Figura 20 Pruebas de viabilidad a las 6 horas de antildeadir diferentes dosis de partiacuteculas A
continuacioacuten se separaron las ceacutelulas se tintildeeron con azul tripaacuten y se contaron las ceacutelulas vivas
o muertas El graacutefico representa ceacutelulas vivas normalizadas y la desviacioacuten estaacutendar de tres
experimentos No se encuentran diferencias
0
02
04
06
08
1
12
14
16
Control W-F68
10microlml
W-F68
5microlml
O-F68
10microlml
O-F68 5microlml
Viability after 6 hours
Viabilidad 6 horas despueacutes
96
5FORMULACIOacuteN CARACTERIZACIOacuteN COLOIDAL Y EFECTO
BIOLOacuteGICO IN VITRO DE NANOPARTIacuteCULAS DE PLGA
CARGADAS CON BMP-2 PARA LA REGENERACIOacuteN OacuteSEA
51 Antecedentes
En el contexto de la nanomedicina la regeneracioacuten de tejidos usando micro y nano estructuras
coloidales que tienen un tamantildeo y actividad superficial uacutenicos ha recibido una atencioacuten creciente
en los uacuteltimos antildeos Se han realizado muchos esfuerzos para mejorar la ingenieriacutea de estos nano-
sistemas con el fin de alcanzar una entrega inteligente de moleacuteculas bioactivas para optimizar
sus ventajas terapeacuteuticas y minimizar los efectos secundarios nocivos (van Rijt and Habibovic
2017) Con este objetivo hay descrito un amplio espectro de nanoportadores biocompatibles que
muestran propiedades adecuadas para diferentes aplicaciones bioloacutegicas y terapeacuteuticas (Kumar et
al 2017) Entre estas variadas propuestas los nanosistemas polimeacutericos representan un grupo
importante siendo el PLGA uno de los maacutes utilizados debido a las propiedades comentadas
anteriormente a lo largo de este trabajo entre ellas destaca su biocompatibilidad
biodegradabilidad y baja citotoxicidad asi como su capacidad para administrar una amplia
variedad de moleacuteculas y faacutermacos activos moleacuteculas sinteacuteticas o naturales con propiedades
hidrofiacutelicas o hidrofoacutebicas y biomoleacuteculas de proteiacutenas a aacutecidos nucleicos (Danhier et al 2012)
(Ding and Zhu 2018) (Arias et al 2015) obteniendo la aprobacioacuten de diferentes agencias
farmaceacuteuticas para uso humano (Mir Ahmed and Rehman 2017) (Jana and Jana 2017)
Dentro de la ingenieriacutea de tejidos en el campo de la odontologiacutea las teacutecnicas de regeneracioacuten
oacutesea empiezan a despertar un gran intereacutes por parte de los diferentes profesionales de la salud
contribuyendo con ello a un mayor desarrollo de estas liacuteneas de investigacioacuten y generando una
mayor tendencia a diferentes viacuteas de crecimiento relacionadas con el subsanamiento de los
obstaacuteculos que pueden originarse durante el proceso de encapsulacioacuten de moleacuteculas hidrofilicas
o en el de funcionalizacioacuten especiacutefica de la superficie a fin de mejorar la versatilidad de las
diferentes moleculas supuestos eacutestos en los que el PLGA es el poliacutemero de referencia para la
97
comunidad cientiacutefica en aras de crear NP para favorecer la cicatrizacioacuten oacutesea (Bapat et al 2019)
La literatura describe el suministro de moleacuteculas bioactivas normalmente factores de crecimiento
utilizando micropartiacuteculas polimeacutericas (MP) y NP con PLGA como componente principal
(Ortega-Oller et al 2015) Entre los factores de crecimiento morfogeneacutetico oacuteseo la BMP-2
(proteiacutena morfogeneacutetica oacutesea 2) ha sido la maacutes citada con muchos ejemplos en los que la
encapsulacioacuten o la adsorcioacuten en la superficie permite una eficiencia de atrapamiento adecuada y
diversos patrones de liberacioacuten (Ji et al 2010) (Kirby et al 2011) (Qutachi Shakesheff and
Buttery 2013) (Wang et al 2015) (Zhang et al 2016) Para las proteiacutenas con una vida media
muy corta como las BMP los nanosistemas PLGA biodegradables proporcionan proteccioacuten y
una dosis oacuteptima para una estimulacioacuten adecuada de la diferenciacioacuten celular (Begam et al 2017)
(Balmayor et al 2009)
Por lo tanto dentro de este escenario en el presente trabajo buscamos optimizar un sistema
de nanopartiacuteculas para llevar a cabo y controlar la liberacioacuten de BMP-2 utilizando como punto de
partida el procedimiento de siacutentesis de un sistema de NP cargado de lisozima previamente
descrito para la encapsulacioacuten de ese modelo de proteiacutena (Ortega-Oller et al 2017) Ademaacutes
para encapsular BMP-2 preparamos un segundo sistema en el que esta proteiacutena se co-adsorbioacute
con albuacutemina de suero bovino en la superficie de NP vaciacuteas Hemos estudiado el tamantildeo y la
morfologiacutea la eficiencia de la encapsulacioacuten de proteiacutenas las caracteriacutesticas de la superficie y la
estabilidad coloidal y temporal para completar la caracterizacioacuten fisicoquiacutemica de ambos sistemas
NP
El perfil de liberacioacuten de BMP-2 indica el potencial de un nanoportador PLGA para la
regeneracioacuten oacutesea y depende en gran medida de la degradacioacuten del poliacutemero por hidroacutelisis (Xu et
al 2017) Sin embargo a corto plazo durante el cual la liberacioacuten no depende de esta degradacioacuten
quiacutemica es necesario un control adecuado de la liberacioacuten para modular otros procesos fiacutesicos
Por lo tanto enfocamos nuestros experimentos de liberacioacuten a corto plazo utilizando diferentes
teacutecnicas para comparar las dos muestras de NP y establecer los perfiles de liberacioacuten de BMP-2
98
correspondientes Finalmente la actividad bioloacutegica (migracioacuten celular proliferacioacuten y
diferenciacioacuten osteogeacutenica) se proboacute in vitro utilizando ceacutelulas estromales mesenquimales (MSC)
derivadas del hueso alveolar (Padial-Molina et al 2019)
52 Materiales y Meacutetodos
521Siacutentesis de nanoparticulas
14Formulacioacuten
El aacutecido poli (lactico-co-glicolico) (PLGA 5050) ([C2 H2 O2]x [C3 H4 O2]y) x = 50 y = 50
(Resomerreg 503H) 32 - 44 kDa se usoacute como poliacutemero El surfactante polimeacuterico Pluronicreg F68
(Poloxaacutemero 188) (Sigma-Aldrich) se usoacute como el emulsionante Su estructura se basa en un
copoliacutemero tri-bloque de poli (oacutexido de etileno) PEO y poli (oacutexido de propileno) PPO (PEO)a ndash
(PPO)b ndash (PEO)a con a = 75 y b = 30 La lisozima de huevo de gallina (Sigma-L7651) se usoacute
como proteiacutena hidroacutefila La proteiacutena morfogeneacutetica oacutesea recombinante humana rhBMP-2
(Sigma-H4791) se utilizoacute como biomoleacutecula terapeacuteutica El agua se purificoacute en un sistema Milli-
Q Academic Millipore Se usoacute un meacutetodo de siacutentesis de doble emulsioacuten siguiendo un
procedimiento previamente descrito con ligeras modificaciones (Ortega-Oller et al 2017) En
este meacutetodo se disolvieron 100 mg de PLGA y 3 mg de aacutecido desoxicoacutelico (DC) en un tubo que
conteniacutea 1 ml de acetato de etilo (EA) y se sometieron a voacutertice En total se agregaron 40 μL de
una solucioacuten tamponada a pH 128 con o sin rhBMP-2 (200 μg mL) y se sonicoacute de inmediato
(Branson Ultrasonics 450 Analifier Sonifier) durante 1 min (Dial del ciclo de trabajo 20 Dial
de control de salida 4) con el tubo rodeado de hielo Esta emulsioacuten primaria de WO se vertioacute en
un tubo de plaacutestico que conteniacutea 2 ml de una solucioacuten tamponada (pH 12) de F68 a 1 mg ml y
se agitoacute en voacutertex durante 30 s Luego el tubo rodeado de hielo se sonicoacute a la amplitud maacutexima
de la micro punta durante 1 minuto (control de salida 7) Esta segunda emulsioacuten WOW se vertioacute
en un vaso que conteniacutea 10 ml de la solucioacuten F68 tamponada y se mantuvo bajo agitacioacuten
magneacutetica durante 2 minutos El disolvente orgaacutenico se extrajo raacutepidamente por evaporacioacuten al
99
vaciacuteo hasta un volumen final de 8 ml Los sistemas de NP encapsulados vaciacuteos y BMP-2
resultantes se denominaron NP y NP-BMP2 respectivamente En la Figura 21 se muestra un
esquema detallado del procedimiento de siacutentesis con un rendimiento basado en el componente
PLGA siempre superior al 85
15Limpieza y almacenamiento
Despueacutes de la evaporacioacuten del solvente orgaacutenico la muestra se centrifugoacute durante 10 minutos
a 20 deg C a 12000 rpm El sobrenadante se filtroacute usando nanofiltros Millipore 01 μm para medir
la proteiacutena libre no encapsulada El sedimento se resuspendioacute en tampoacuten fosfato (NaH2PO4 115
mM) PB hasta un volumen final de 4 ml y se mantuvo refrigerado a 4ordmC En estas condiciones
los sistemas mantuvieron la estabilidad coloidal al menos durante un mes
16Carga de proteiacutenas y eficiencia de encapsulacioacuten
La carga inicial de proteiacutenas se optimizoacute para la formulacioacuten de nanopartiacuteculas preservando
la estabilidad coloidal final despueacutes de la etapa de evaporacioacuten y teniendo en cuenta las cantidades
mostradas en la literatura para este factor de crecimiento cuando se encapsula dentro de NPs de
PLGA (drsquoAngelo et al 2010) (Chang et al 2017) Por lo tanto elegimos 2 μg como la masa total
inicial de rhBMP-2 lo que significa una relacioacuten de 2 10 5 p p (rhBMP-2 PLGA) La
cantidad de rhBMP-2 encapsulado se calculoacute midiendo la diferencia entre la cantidad agregada
inicial y la proteiacutena libre no encapsulada presente en el sobrenadante despueacutes de la etapa de
limpieza que se proboacute mediante un ensayo inmuno-absorbente ligado a enzimas especiacuteficas
siguiendo las instrucciones del fabricante (ELISA kit RAB0028 de Sigma-Aldrich St Louis
MO EE UU) Luego la eficiencia de encapsulacioacuten de proteiacutenas (EE) se calculoacute de la siguiente
manera
EE = 119872119868minus119872119865
119872119868119909 100
donde MI es la masa total inicial de rhBMP-2 y MF es la masa total de rhBMP-2 en el
sobrenadante acuoso
100
17Adsorcioacuten Fiacutesica de Proteiacutenas
La albuacutemina de suero bovino (BSA) y la rhBMP-2 se acoplaron en la superficie de
nanopartiacuteculas vaciacuteas mediante un meacutetodo de adsorcioacuten fiacutesica El volumen apropiado de una
solucioacuten de proteiacutena acuosa que conteniacutea 05 mg de BSA y 2 μg de rhBMP-2 se mezcloacute con 5 ml
de tampoacuten de acetato (pH 5) que conteniacutea NP vaciacuteas con 125 mg de PLGA Esto proporcionoacute
una cantidad inicial de proteiacutenas correspondiente al 004 p p (proteiacutena PLGA) mientras que
la relacioacuten de masa entre proteiacutenas fue de 04 p p (rhBMP-2 BSA) Esta solucioacuten se incuboacute a
temperatura ambiente durante 2 h con agitacioacuten mecaacutenica Las nanopartiacuteculas se separaron de la
solucioacuten de tampoacuten por centrifugacioacuten y despueacutes de que se filtraran los sobrenadantes
(nanofiltros Millipore 01 μm) se analizaron cualitativamente por electroforesis en gel mientras
que la cuantificacioacuten de proteiacutenas se realizoacute mediante un ensayo de proteiacutena de aacutecido
bicinconiacutenico (BCA) (Sigma-Aldrich St Louis MO EE UU) Para BSA y el ELISA especiacutefico
para rhBMP-2 El sedimento de nanopartiacuteculas se resuspendioacute en tampoacuten fosfato (pH 74) y se
almacenoacute a 4ordmC Este sistema se denominoacute NP-BSA-BMP2
18Separacioacuten de proteiacutenas por electroforesis en gel SDS-PAGE
Las NP cargadas de proteiacutena y los diferentes sobrenadantes se trataron a 90 deg C durante 10
minutos en el siguiente tampoacuten Tris-HCl 625 mM (pH 68 a 25 deg C) dodecil sulfato de sodio al
2 (p v) (SDS) 10 de glicerol 001 (p v) de azul de bromofenol ditiotreitol (DTT) 40
mM Las muestras se separaron luego por tamantildeo en gel de poliacrilamida poroso al 12
(electroforesis en gel de poliacrilamida SDS 1D) bajo el efecto de un campo eleacutectrico La
electroforesis se realizoacute a voltaje constante (130 V 45 min) y los geles se tintildeeron usando una
solucioacuten de azul de Coomassie (01 de azul brillante de Coomassie R-250 50 de metanol y
10 de aacutecido aceacutetico glacial) y se destintildeeron con la misma solucioacuten que carece del tinte
101
522 Caracterizacioacuten de nanopartiacuteculas morfologiacutea tamantildeo concentracioacuten y movilidad
electrocineacutetica
Se obtuvieron imaacutegenes de NP mediante microscopiacutea electroacutenica de barrido (SEM) con un
microscopio electroacutenico de barrido de emisioacuten de campo SUPRA 40VP Zeiss del Centro de
Instrumentacioacuten Cientiacutefica de la Universidad de Granada (CIC UGR)
La distribucioacuten del tamantildeo hidrodinaacutemico de las NP se evaluoacute mediante anaacutelisis de
seguimiento de nanopartiacuteculas (NTA) con un NanoSight LM10-HS (GB) FT14 (NanoSight
Amesbury Reino Unido) y una caacutemara sCMOS La concentracioacuten de partiacuteculas de acuerdo con
el diaacutemetro (distribucioacuten de tamantildeos) se calculoacute como un promedio de al menos tres
distribuciones de tamantildeos independientes La concentracioacuten total de NP de cada sistema se
determinoacute para controlar el nuacutemero de partiacuteculas utilizadas en los experimentos celulares Las
condiciones de medicioacuten para todas las muestras fueron 25 deg C una viscosidad de 089 cP un
tiempo de medicioacuten de 60 s y una ganancia de caacutemara de 250 El obturador de la caacutemara fue de
11 y 15 ms para las NP vaciacuteas y cargadas con BMP respectivamente El umbral de deteccioacuten se
fijoacute en 5
La movilidad electroforeacutetica de las NP se determinoacute utilizando un dispositivo Zetasizerreg
NanoZeta ZS (Malvern Instrument Ltd Malvern Reino Unido) que funciona a 25 deg C con un
laacuteser He-Ne de 633 nm y un aacutengulo de dispersioacuten 173 Cada punto de datos se tomoacute como un
promedio sobre tres mediciones de muestra independientes Para cada muestra la distribucioacuten de
movilidad electroforeacutetica y la movilidad electroforeacutetica promedio (μ-promedio) se determinaron
mediante la teacutecnica de electroforesis Doppler laacuteser
523 Estabilidad coloidal y temporal en medios bioloacutegicos
El diaacutemetro hidrodinaacutemico promedio y el iacutendice de polidispersidad (PDI) por dispersioacuten
dinaacutemica de la luz (DLS) de cada sistema de NP se midieron en diferentes medios (tampoacuten de
fosfato (PB) tampoacuten de fosfato salino (PBS) y medio de cultivo celular Medio de Eagle
modificado de Dulbecco DMEM (Sigma)) Ademaacutes los datos sobre la estabilidad temporal se
102
obtuvieron repitiendo estos anaacutelisis en diferentes momentos despueacutes de la siacutentesis (0 1 y 5 diacuteas)
y despueacutes de 1 mes en condiciones de almacenamiento
Los experimentos de liberacioacuten in vitro se realizaron de la siguiente manera 1 ml de cada
muestra para cada tiempo de incubacioacuten se suspendioacute en PBS a 37 deg C Despueacutes del tiempo
correspondiente (24 48 96 168 h) las NP se separaron del sobrenadante de las proteiacutenas
liberadas por centrifugacioacuten durante 10 min a 14000 rpm (10 C) El sedimento de NP se suspendioacute
en 1 ml de NaOH 005 M y se agitoacute durante 2 h para una degradacioacuten completa del poliacutemero La
solucioacuten de proteiacutena alcalina se analizoacute mediante BCA y ELISA para cuantificar la cantidad
ineacutedita La proteiacutena liberada se calculoacute teniendo en cuenta la cantidad encapsulada total Todos
los experimentos se realizaron por triplicado
524 Interacciones celulares
Para todos los estudios bioloacutegicos in vitro se utilizoacute una poblacioacuten celular cultivada del hueso
alveolar maxilar Esta poblacioacuten se caracterizoacute previamente y se confirmoacute que presentaba todas
las caracteriacutesticas de una poblacioacuten de ceacutelulas del estroma mesenquimatoso (MSC) (Padial-
Molina et al 2019) Las ceacutelulas se tomaron de donantes humanos sanos despueacutes de la aprobacioacuten
del Comiteacute de Eacutetica para la Investigacioacuten Humana de la Universidad de Granada (424 CEIH
2018) Medio de Eagle modificado por Dulbecco regular (DMEM) con 1 g L de glucosa
(DMEM-LG) (Gibco) suero bovino fetal al 10 (FBS) (Sigma-Aldrich St Louis MO EE UU)
1 100 de aminoaacutecidos no esenciales (NEAA) (Gibco) 001 μg ml de factor de crecimiento de
fibroblastos baacutesico (bFGF) (PeproTech Londres Reino Unido) 100 U ml de penicilina
estreptomicina y 025 μg ml de anfotericina B utilizado como medio de cultivo para todos los
experimentos Los cultivos se mantuvieron a 37 deg C en una atmoacutesfera de CO2 al 5 (2000 ceacutelulas
pocillo) Todos los experimentos bioloacutegicos se repitieron por triplicado al menos 3 veces por
condicioacuten
19Migracioacuten Celular
103
Un ensayo de migracioacuten celular se realizoacute como se describioacute anteriormente (Padial-Molina
Volk and Rios 2014) (Liang Park and Guan 2007) Brevemente las MSC se distribuyeron en
tres pocillos para cada condicioacuten y se les permitioacute crecer hasta una confluencia celular cercana al
99 en 24 pocillos placa de 3000 ceacutelulas cm2 y en cada pocillo se realizaron tres rasguntildeos
diferentes Luego las ceacutelulas se privaron de hambre durante 24 h mediante la adicioacuten de medio
de cultivo sin suero Se hizo un rasguntildeo usando una punta de pipeta a lo largo del diaacutemetro del
pozo Se realizoacute un paso de lavado con PBS para eliminar las ceacutelulas rayadas Se antildeadieron nuevos
medios de cultivo completos y se suplementaron seguacuten el grupo asignado (BMP-2 NP-BMP2 y
NP-BSA-BMP2 a 125 25 y 5 ng ml de BMP-2) Posteriormente se tomaron nueve imaacutegenes
de la misma aacuterea en cada condicioacuten hasta 48 h maacutes tarde En estas imaacutegenes el aacuterea raspada se
midioacute con el software ImageJ (Instituto Nacional de Salud Bethesda MD EUA
(httprsbwebnihgovij) La reduccioacuten en el aacuterea rayada con el tiempo se midioacute considerando el
aacuterea en el tiempo 0 como 100 abierta
20Proliferacioacuten celular
La proliferacioacuten se evaluoacute mediante un ensayo de sulforhodamina (SRB) (Houghton et al
2007) El ensayo se realizoacute sembrando las ceacutelulas a 1500 ceacutelulas cm2 en una placa de 96 pocillos
a una confluencia no superior al 50 Despueacutes de la unioacuten celular se agregaron los diferentes
suplementos (BMP-2 NP-BMP2 y NP-BSA-BMP2 a 125 25 y 5 ng ml de BMP-2) y las ceacutelulas
se mantuvieron en cultivo durante 7 diacuteas En cada punto de tiempo las ceacutelulas se lavaron con PBS
1X y se fijaron antildeadiendo aacutecido tricloroaceacutetico al 10 enfriado con hielo durante 20 minutos a
4ordmC Luego las ceacutelulas se lavaron 3 veces con dH2O y se secaron hasta que se recogieron todos
los puntos de tiempo Cada pocillo recibioacute 04 de SRB en 1 de aacutecido aceacutetico durante 20
minutos a temperatura ambiente con agitacioacuten suave La tincioacuten se terminoacute lavando cada pocillo
3 veces con aacutecido aceacutetico al 1 y secaacutendolo a temperatura ambiente durante 24 h El colorante se
recuperoacute de las ceacutelulas antildeadiendo Tris Base 10 mM a pH 105 y agitando suavemente durante 10
104
minutos La solucioacuten recuperada se distribuyoacute luego en una placa de 96 pocillos y se leyoacute la
absorbancia oacuteptica a 492 nm
bullDiferenciacioacuten osteogeacutenica
La diferenciacioacuten osteogeacutenica se evaluoacute mediante la adicioacuten de medios osteogeacutenicos al cultivo
celular en combinacioacuten con BMP-2 NP-BMP2 y NP-BSA-BMP2 libres a las dosis maacutes altas
utilizadas en experimentos anteriores Las ceacutelulas se sembraron a 3000 ceacutelulas cm2 y se
cultivaron para alcanzar una confluencia del 85 al 90 Esto fue seguido por la adicioacuten de
medios de induccioacuten que conteniacutean 10 mM de glicerofosfato (Fluka 50020) 01 μM de
dexametasona (Sigma-Aldrich D2915) y 005 mM de aacutecido L-ascoacuterbico (Sigma-Aldrich
A8960) Los cultivos celulares se mantuvieron durante 7 diacuteas para analizar la actividad temprana
En el diacutea 7 las ceacutelulas se recogieron en 1 ml de TRIzolreg Luego se extrajo el ARN y se convirtioacute
en ADNc Luego se evaluoacute la fosfatasa alcalina (ALP) y se calculoacute la expresioacuten con respecto a
la proteiacutena gliceraldehiacutedo-3-fosfato deshidrogenasa (GAPDH) por el meacutetodo 2 DDCt Estos
procedimientos se llevaron a cabo como describe en otra parte el siguiente autor (Padial-Molina
et al 2019) Las secuencias de cebador directo e inverso fueron
AGCTCATTTCCTGGTATGACAAC y TTACTCCTTGGAGGCCATGTG para GAPDH
TCCAGGGATAAAGCAGGTCTTG y CTTTCTCTTTCTCTGGCACTAAGG para ALP
bullEvaluacioacuten estadiacutestica
La migracioacuten y la proliferacioacuten celular se evaluaron mediante ANOVA seguido de la prueba
de comparaciones muacuteltiples de Tukey para el anaacutelisis por pares La comparacioacuten entre los niveles
de ALP a los 4 frente a los 7 diacuteas se analizoacute mediante un par de pruebas t de Student En todos los
casos se establecioacute un valor p inferior a 005 como significacioacuten estadiacutestica
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53 Resultados y Discusioacuten
531Formulacioacuten de nanoparticulas
La evaporacioacuten de doble emulsioacuten-solvente ha sido descrita como un meacutetodo robusto y de uso
frecuente para producir NP de PLGA cargadas con biomoleacuteculas (Ding and Zhu 2018)
(McClements 2018) (Ortega-Oller et al 2015) (Iqbal et al 2015) Una formulacioacuten previamente
optimizada por nuestro grupo permitioacute la preservacioacuten de la actividad bioloacutegica de biomoleacuteculas
encapsuladas usando un solvente orgaacutenico ligeramente agresivo Ademaacutes el aacutecido desoxicoacutelico
se ha utilizado en el primer paso de la formulacioacuten para mejorar la estabilidad coloidal de las NP
y simultaacuteneamente para obtener superficies de NP enriquecidas con grupos carboxiacutelicos
mejorando su versatilidad y permitiendo que un quiacutemico posterior de lugar a la inmovilizacioacuten de
diferentes ligandos especiacuteficos (Sanchez-Moreno et al 2013) Por medio de esta formulacioacuten
mejorada en el presente trabajo desarrollamos nanopartiacuteculas vaciacuteas (NP) o nanopartiacuteculas que
encapsulan rhBMP-2 (NP-BMP2)
106
Una descripcioacuten esquemaacutetica del procedimiento de siacutentesis se muestra en la Figura 21
Figura 21 Esquema de la formulacioacuten de NP-BMP2
Para NP-BMP2 logramos una eficiencia de encapsulacioacuten de proteiacutenas (EE) de 97 plusmn 2 Este
resultado es estable en la literatura en la que varios autores han reportado valores igualmente altos
que encapsulan esta proteiacutena dentro de nanopartiacuteculas y micropartiacuteculas de PLGA (Lochmann et
al 2010) (Kempen et al 2008) Nuestra formulacioacuten tiene varios factores que conducen a este
elevado valor de EE La baja relacioacuten proteiacutena poliacutemero en masa (Manuel J Santander-Ortega
Csaba et al 2010) la afinidad de rhBMP-2 a una interaccioacuten inespeciacutefica con superficies
hidrofoacutebicas (Lochmann et al 2010) o la adicioacuten de estabilizadores (poloxaacutemero) en el segundo
paso del procedimiento de doble emulsioacuten (Ortega-Oller et al 2015) La ausencia de rhBMP-2
en el sobrenadante resultante de la etapa de centrifugacioacuten en el proceso de limpieza se verificoacute
mediante ELISA y SDS-PAGE en el que se muestra una banda clara correspondiente a 14 kD de
cadenas polipeptiacutedicas rhBMP-2 para el carril A en la Figura 22 correspondiente a NP-BMP2
La masa de proteiacutena encapsulada alrededor de 2 microg es similar a la de diferentes micro y
107
nanosistemas PLGA descritos en la literatura (Wang et al 2015) (Chung et al 2007) (La et al
2010) Teniendo en cuenta las condiciones de almacenamiento para nuestras muestras esto
corresponde a 500 ng mL lo que representa una cantidad suficiente de concentracioacuten para
aplicaciones praacutecticas ya que este factor de crecimiento muestra actividades bioloacutegicas in vitro en
dosis muy bajas (5ndash20 ng ml) (Ortega-Oller et al 2015)
Figura 22 Anaacutelisis de electroforesis en gel de SDS-poliacrilamida (SDS-PAGE) en
condiciones reductoras de Nanopartiacuteculas de PLGA soacutelidas (NP de PLGA) y fracciones liacutequidas
(sobrenadante) de diferentes sistemas de NP Carril P proteiacutenas estaacutendares carril A NP-BMP2
(proteiacutena morfogeneacutetica oacutesea) carril B sobrenadante de NP-BMP2 despueacutes de la siacutentesis y
encapsulacioacuten de rhBMP-2 carril C NP despueacutes de la adsorcioacuten fiacutesica de BSA rhBMP-2 carril
D sobrenadante despueacutes de la adsorcioacuten fiacutesica de BSA (albuacutemina de suero bovino) rhBMP-2
en el sistema NP
Por otro lado resultoacute un segundo nanosistema modificando la forma en que rhBMP-2 es
incorporado en el nanoportador Hay varios ejemplos de adsorcioacuten superficial de diferentes
factores de crecimiento en micro y nanopartiacuteculas (La et al 2010) (Fu et al 2012) (Rahman et
al 2014) y la inmovilizacioacuten de la superficie sobre la encapsulacioacuten recientemente se ha
108
propuesto como una forma de modular la liberacioacuten posterior de biomoleacuteculas Este proceso que
depende de la lenta difusioacuten de las biomoleacuteculas a traveacutes de la matriz polimeacuterica estaacute en
consecuencia altamente influenciado por la interaccioacuten proteiacutena-poliacutemero (Pakulska et al 2016)
(Fu et al 2017) y degradacioacuten del poliacutemero (Mir Ahmed and Rehman 2017) (Ding and Zhu
2018) Por lo tanto este nuevo enfoque en el uso de NP de PLGA para el suministro de
biomoleacuteculas se exploroacute inmovilizando la proteiacutena rhBMP-2 en la superficie de las NP vaciacuteas
mediante una simple adsorcioacuten fiacutesica Se sabe que este proceso se rige por interacciones
electrostaacuteticas e hidrofoacutebicas entre las moleacuteculas de proteiacutenas y las superficies NP (Peula and de
las Nieves 1993)
Para esto los grupos cargados en la superficie la hidrofilia la carga neta de las moleacuteculas de
proteiacutena y las caracteriacutesticas del medio de adsorcioacuten son los paraacutemetros de referencia Por lo
tanto disentildeamos un experimento de co-adsorcioacuten en el que interactuacutea una mezcla de rhBMP-2 y
BSA (04 p p rhBMP-2 BSA) simultaacuteneamente con la superficie de NP de PLGA Las
albuacuteminas se usan habitualmente como proteiacutenas protectoras cuando los factores de crecimiento
se incorporan en las NPs de PLGA (Ortega-Oller et al 2015) (Zhang et al 2016) Ademaacutes una
distribucioacuten superficial de las moleacuteculas de BSA puede mejorar la estabilidad coloidal de las NP
a pH fisioloacutegico debido a su carga negativa neta bajo estas condiciones (Peula and de las Nieves
1994) La Figura 23 muestra un esquema del proceso de co-adsorcioacuten La eficiencia de adsorcioacuten
es superior al 95 y en el SDS-PAGE de la Figura 22 se pueden ver dos bandas caracteriacutesticas
de ambas proteiacutenas en el carril C correspondiente al nanosistema NP-BSA-BMP2
109
Figura 23 Esquema del proceso de adsorcioacuten de proteiacutenas para NP-BSA-BMP2
Sin embargo el carril D correspondiente a la ejecucioacuten del sobrenadante desde la
centrifugacioacuten del nanosistema despueacutes de los procesos de adsorcioacuten muestra la ausencia de
cualquier proteiacutena Este resultado se explica completamente teniendo en cuenta el pH del medio
(pH 50) cerca del punto isoeleacutectrico de BSA donde la adsorcioacuten de esta proteiacutena en
nanopartiacuteculas cargadas negativamente presenta un maacuteximo (Peula and de las Nieves 1993)
(Peula Hidalgo-Alvarez and de las Nieves 1995) La inmovilizacioacuten de rhBMP-2 en la superficie
cargada negativamente de las NPs demuestra que estaacuten favorecidos electrostaacuteticamente debido a
la carga neta positiva de esta proteiacutena a pH aacutecido y neutro
532Caracterizacioacuten de nanopartiacuteculas
21Tamantildeo de nanopartiacuteculas
Micrografiacuteas SEM y STEM (Figura 24) muestran que las muestras consisten en partiacuteculas
esfeacutericas de diaacutemetros diferentes (entre 150 y 450 nm) un rango similar al encontrado en un
trabajo anterior en el que las NP se cargaron con lisozima siguiendo un protocolo de siacutentesis
similar (Ortega-Oller et al 2017) En ese trabajo la teacutecnica DLS no pudo proporcionar una
distribucioacuten de tamantildeo confiable Por lo tanto la teacutecnica NTA fue directamente utilizada para
determinar el tamantildeo hidrodinaacutemico de las NP cargadas con BMP2
Las distribuciones de tamantildeo para NP vaciacuteas (NP) y cargadas con BMP (NP-BMP2) de NTA
(Figura 25 y videos S1 S2) fueron consistentes con las imaacutegenes SEM Se encontroacute que las
partiacuteculas con diaacutemetros entre 100 y 500 nm teniacutean la concentracioacuten de partiacuteculas maacutes alta en
110
alrededor de 200 nm La carga con BMP tuvo un efecto en la distribucioacuten del tamantildeo lo que
condujo a picos maacutes definidos Estas mediciones nos permitieron determinar la concentracioacuten de
partiacuteculas en la muestra medida 688 plusmn 009 x 10 8 pp mL y 519 plusmn 012 x10 8 pp mL para
nanosistemas NP y NP-BMP2 respectivamente Estos valores fueron utilizados (teniendo en
cuenta la dilucioacuten correspondiente) para controlar el nuacutemero de partiacuteculas antildeadidas en los
experimentos celulares
Figura 24 Micrografiacutea de microscopiacutea electroacutenica de barrido (SEM) de nanopartiacuteculas
cargadas con rhBMP-2 (NP-BMP2)
111
Figura 25 Distribucioacuten del diaacutemetro hidrodinaacutemico de NP (ciacuterculos) y NP-BMP2 (liacutenea
negra gruesa) medidos a pH 70 (tampoacuten de fosfato) por anaacutelisis de seguimiento de
nanopartiacuteculas (NTA)
bullMovilidad electrocineacutetica y estabilidad coloidal
La carga superficial de las nanopartiacuteculas se puede analizar mediante un estudio electrocineacutetico
midiendo la movilidad electroforeacutetica (microe) en diferentes condiciones La Figura 26 muestra la
movilidad electroforeacutetica y el potencial zeta evaluado para los tres nanosistemas NP NP- BMP2
y NP-BSA-BMP2 a baja fuerza ioacutenica y diferentes valores de pH La carga superficial eleacutectrica
de las NP reside en los grupos carboxiacutelicos de las no cubiertas por PLGA y moleacuteculas de aacutecido
desoxicoacutelico Estos grupos funcionalizados tambieacuten son uacutetiles debido a la posibilidad de una
vectorizacioacuten quiacutemica de la superficie para desarrollar nanoportadores de entrega dirigida
(Siafaka et al 2016)
Se confirmoacute previamente que la protonacioacuten de estos grupos de superficies aacutecidas a valores de
pH bajo su valor de pKa estaba estrechamente relacionado con una peacuterdida de carga superficial
y en consecuencia una reduccioacuten (en valor absoluto) de la movilidad electroforeacutetica del sistema
coloidal (Peula-Garciacutea Hidalgo-Alvarez and De Las Nieves 1997) (Manuel J Santander-Ortega
Lozano-Loacutepez et al 2010) Por lo general cuando las partiacuteculas coloidales estaacuten recubiertas por
Conce
ntr
acioacute
n d
e par
tiacutecu
las
(10
6 p
pm
L)
112
moleacuteculas de proteiacutena los valores de microe cambian notablemente en comparacioacuten con el mismo
valor de las superficies desnudas y estaacuten influenciadas por la carga eleacutectrica de las moleacuteculas de
proteiacutena adsorbidas (Peula-Garcia Hidaldo-Alvarez and De las Nieves 1997) (Santander-Ortega
Bastos-Gonzalez and Ortega-Vinuesa 2007) El comportamiento electrocineacutetico del sistema NP-
BMP2 sigue siendo similar al de NP y la encapsulacioacuten de rhBMP-2 no afecta la distribucioacuten de
carga superficial DAngelo y colaboradores informaron de un resultado similar al encapsular
diferentes factores de crecimiento en nanopartiacuteculas de mezcla de PLGA-poloxaacutemero en la
misma proporcioacuten p p de proteiacutena poliacutemero (drsquoAngelo et al 2010) Esto puede deberse a la
baja cantidad de proteiacutena encapsulada y su distribucioacuten en la parte interna de las NP (lejos de la
superficie) En nuestro sistema la distribucioacuten interna puede verse favorecida por las condiciones
de encapsulacioacuten donde el pH baacutesico (pH 120) del agua contiene rhBMP-2 que permite una carga
negativa de estas moleacuteculas de proteiacutena evitando asiacute su interaccioacuten electrostaacutetica especiacutefica con
grupos aacutecidos de las NP
113
Figura 26 Movilidad electroforeacutetica y potencial zeta versus pH en medios de baja salinidad
(fuerza ioacutenica igual a 0002 M) para los diferentes nanosistemas (cuadrado negro) NP
(triaacutengulo azul) NP-BMP2 (ciacuterculo rojo) NP-BSA-BMP2
La distribucioacuten electrocineacutetica para el sistema NP-BSA-BMP2 cambia radicalmente Como se
mostroacute anteriormente la eficiencia de adsorcioacuten muy alta conduce a NP con ambas proteiacutenas
adsorbidas alrededor de su superficie Esta situacioacuten estaacute estrechamente relacionada con los
valores microe de la Figura 26 Teniendo en cuenta la relacioacuten p p entre proteiacutenas adsorbidas (250
veces mayor para BSA) las moleacuteculas de albuacutemina modulan el comportamiento a valores de pH
por debajo de su punto isoeleacutectrico (pI 47) donde la carga neta positiva BSA enmascara la carga
superficial original de las NP e incluso cambia sus valores originales a valores positivos Esto es
un resultado tiacutepico encontrado para estas partiacuteculas coloidales que cubren proteiacutenas (Peula
Hidalgo-Alvarez and de las Nieves 1995) (Peula JM Callejas J de las Nieves 1994) A
valores de pH neutros y baacutesicos las moleacuteculas de BSA tienen una carga neta negativa y la ligera
disminucioacuten en los valores absolutos microe podriacutea ser debido a la reduccioacuten de la carga superficial
neta negativa de los NP que pueden estar protegidos al menos en una pequentildea parte por la carga
positiva de las moleacuteculas de rhBMP-2 bajo su punto isoeleacutectrico baacutesico (pI 90)
Pote
nci
al z
eta
(mV
)
114
La estabilidad coloidal para los diferentes nanosistemas (NP NP-BMP2 y NP-BSA-BMP2)
fue determinada mediante el anaacutelisis de las distribuciones de tamantildeo en varios medios (PB PBS
y DMEM) en diferentes tiempos despueacutes de la siacutentesis (0 1 y 5 diacuteas) Se encontraron
distribuciones de tamantildeo similares a las originales para las dos formulaciones NP y NP-BMP2
en todos los medios analizados Este resultado fue similar al encontrado previamente para estos
tipos de NP que encapsulan la lisozima (Ortega-Oller et al 2017) en el que la combinacioacuten de
las interacciones electrostaacuteticas y esteacutericas generadas por grupos quiacutemicos superficiales de NP
confieren estabilidad al mecanismo que evita la agregacioacuten coloidal (Manuel J Santander-Ortega
Csaba et al 2010) La disminucioacuten del valor absoluto del potencial zeta para el sistema NP-
BSA-BMP2 como consecuencia de la distribucioacuten de proteiacutenas en la superficie no afecta su
estabilidad coloidal Este sistema tambieacuten mantiene la misma distribucioacuten de tamantildeos en los
diferentes medios Se acepta comuacutenmente que un potencial zeta superior a +30 o 30 mV daraacute
lugar a un sistema coloidal estable (Sun 2016) y el valor potencial zeta para NP-BSA-BMP2 es
superior a 30 mV La estabilidad coloidal en PBS y DMEM tiacutepicamente medios utilizados para
el desarrollo de interacciones celulares o scaffolds respectivamente asegura el uso potencial de
estos nanosistemas para entornos de vida in vitro o in vivo Ademaacutes estos sistemas mantuvieron
su tamantildeo bajo almacenamiento en PB a 4 deg C durante al menos 1 mes (datos no mostrados) lo
que demuestra que es un medio adecuado para el almacenamiento de muestras
bullLiberacioacuten de proteiacutenas
Uno de los principales problemas para los micro o nanosistemas de suministro de faacutermacos
PLGA es encontrar el patroacuten de liberacioacuten apropiado para las moleacuteculas de proteiacutenas encapsuladas
unidas Un amplio espectro de formulaciones modula esta propiedad mediante el uso de
diferentes tipos de procesos de siacutentesis poliacutemeros PLGA copoliacutemeros y estabilizadores (Mir
Ahmed and Rehman 2017) (Ortega-Oller et al 2015) Una limitacioacuten y control adecuados en la
liberacioacuten de estallido es fundamental para las BMP a fin de garantizar una liberacioacuten continua a
largo plazo que favorecida por la degradacioacuten del poliacutemero proporcione una mejor accioacuten in vivo
115
para impulsar la regeneracioacuten de hueso y cartiacutelago (Begam et al 2017) Por lo tanto previamente
desarrollamos un nanosistema PLGA dual para la liberacioacuten controlada a corto plazo donde la
difusioacuten de proteiacutenas y la interaccioacuten proteiacutena-poliacutemero son los principales factores que rigen este
proceso (Ortega-Oller et al 2017) En el presente trabajo los nanosistemas NP-BMP2 y NP-
BSA-BMP2 representan dos formas diferentes en las que se incorporoacute rhBMP-2 en el
nanoportador La Figura 27a muestra la liberacioacuten acumulativa de ambas proteiacutenas rhBMP-2 y
BSA para diferentes sistemas en funcioacuten del tiempo en un periacuteodo a corto plazo (7 diacuteas) La
proteiacutena rhBMP-2 encapsulada alcanza una cantidad liberada de alrededor del 30 de la proteiacutena
encapsulada inicial mientras que la rhBMP-2 adsorbida a pesar de su distribucioacuten superficial es
tres veces menor Sin embargo BSA muestra cantidades liberadas de hasta el 80 de los
adsorbidos iniciales En todos los casos las barras de error corresponden a las desviaciones
estaacutendar de tres experimentos independientes En estas condiciones el factor de crecimiento
encapsulado en NP-BMP2 presenta un patroacuten de liberacioacuten similar al encontrado previamente con
la misma formulacioacuten pero usando lisozima como proteiacutena (Ortega-Oller et al 2017) El
poloxaacutemero en la fase acuosa del proceso de siacutentesis puede ser clave para modular las
interacciones proteicas interfaciales especiacuteficas y no especiacuteficas (Del Castillo-Santaella et al
2019) Por lo tanto la relacioacuten entre la interaccioacuten proteiacutena-poliacutemero y la difusioacuten de proteiacutenas
parece estar bien equilibrada evitando un estallido inicial excesivo y al mismo tiempo
manteniendo el flujo de proteiacutenas necesario para liberar alrededor de un tercio de la rhBMP-2
encapsulada en 7 diacuteas Aunque se ha informado ampliamente de un estallido inicial excesivo para
los NP de PLGA relacionados con moleacuteculas de proteiacutenas cercanas a la superficie (Ding and Zhu
2018) esta situacioacuten no aparecioacute para el sistema NP-BMP2 siendo esto consistente con el
comportamiento electrocineacutetico que no mostroacute la presencia de proteiacutena cerca de la superficie La
literatura ofrece algunos ejemplos con liberacioacuten reducida a corto plazo de BMP-2 utilizando maacutes
copoliacutemeros PLGA-PEG hidroacutefilos (Kirby et al 2011) o un proceso de siacutentesis diferente (Chang
et al 2017)
116
El rendimiento de la liberacioacuten del sistema NP-BSA-BMP2 que tambieacuten se muestra en la
Figura 27a presenta diferencias notables El perfil electrocineacutetico ha justificado previamente la
ubicacioacuten de la superficie de BSA y rhBMP-2 en la superficie lo que podriacutea conducir a una
liberacioacuten raacutepida de ambas proteiacutenas Sin embargo los resultados de la Figura 27a y 27b muestran
esta tendencia solo para la proteiacutena BSA que se libera de las NP con aproximadamente el 20
de la cantidad inicial restante despueacutes de siete diacuteas Sin embargo hasta el 90 de la carga inicial
de proteiacutena rhBMP-2 a diferencia de BSA permanece unida a la superficie La superficie NP con
grupos hidrofiacutelicos forma moleacuteculas de poloxaacutemero y una carga negativa debido a la abundante
presencia de grupos carboxiacutelicos (grupos terminales de PLGA y moleacuteculas de aacutecido desoxicoacutelico)
favorecen un proceso de desorcioacuten para BSA cuyas moleacuteculas tienen una carga negativa en
condiciones de liberacioacuten (pH fisioloacutegico) Esto concuerda con los resultados de otros autores
que incluso despueacutes de encapsular BSA en mezclas de PLGA-poloxaacutemero NP logroacute una
descarga de liberacioacuten raacutepida por encima del 40 o 50 de la cantidad inicial de proteiacutena (Manuel
J Santander-Ortega Csaba et al 2010) Ademaacutes la coencapsulacioacuten de albuacuteminas con factores
de crecimiento podriacutea afectar fuertemente su perfil de liberacioacuten causando un estallido inicial
(Balmayor et al 2009) (drsquoAngelo et al 2010) De lo contrario la atraccioacuten electrostaacutetica
especiacutefica entre las moleacuteculas rhBMP-2 positivas y los grupos de superficie negativos ralentiza la
liberacioacuten a corto plazo de esta proteiacutena Este resultado estaacute de acuerdo con la baja liberacioacuten de
BMP adsorbida encontrada previamente usando micro y nanopartiacuteculas de PLGA con grupos
terminales de aacutecido sin tapar (Pakulska et al 2016) (Schrier and DeLuca 2001) Por lo tanto la
combinacioacuten de diferentes meacutetodos para atrapar BMP-2 dentro y alrededor de NP muestra la
posibilidad de lograr una liberacioacuten controlada adecuadamente equilibrando las interacciones
entre poliacutemeros estabilizadores y proteiacutenas
117
(a)
(b)
Figura 27 (a) Liberacioacuten acumulativa de rhBMP-2 para sistemas NP-BMP2 (cuadrado
negro) y NP-BSA-BMP2 (ciacuterculo rojo) y liberacioacuten acumulativa de BSA para el sistema NP-
BSA-BMP2 (triaacutengulo azul) incubado por diferentes tiempos a 37ordmC en tampoacuten de fosfato salino
(pH 74) (b) Anaacutelisis de SDS-PAGE en condiciones reductoras de fraccioacuten soacutelida de NP-BSA-
Lib
erac
ioacuten a
cum
ula
tiva
()
Tiempo (horas)
118
BMP2 despueacutes de la liberacioacuten en diferentes momentos en los que el nuacutemero de cada carril
corresponde al tiempo en horas
533Actividad bioloacutegica e interacciones
bullMigracioacuten Celular
La migracioacuten celular es el primer y necesario paso en la regeneracioacuten de tejidos (Padial-Molina
OrsquoValle et al 2015) Por lo tanto un agente regenerativo debe acelerar la migracioacuten celular o
al menos no interferir con ella En el presente estudio no encontramos diferencias entre los
grupos las dosis y el control en teacuterminos de cierre de un aacuterea rayada (ANOVA con la prueba de
comparaciones muacuteltiples de Tukey) (Figura 28) En contraste con nuestros hallazgos los datos
publicados anteriormente sugieren un efecto positivo de BMP-2 en la migracioacuten celular (Inai et
al 2008) (Gamell et al 2008) Sin embargo en esos estudios las dosis aplicadas y los tipos de
ceacutelulas fueron diferentes a los experimentos actuales Utilizamos dosis maacutes bajas de BMP-2 para
evaluar si incluso a dosis bajas BMP-2 podriacutea proporcionar beneficios si se protegiera en un
sistema de nanopartiacuteculas Como se mencionoacute no demostramos ninguacuten efecto negativo del
sistema en la migracioacuten celular Sin embargo nuestros resultados respaldan la idea de que la
actividad de BMP-2 estaacute mediada por la activacioacuten de la viacutea de la fosfoinositida 3-quinasa (PI3K)
un grupo comuacuten de moleacuteculas de sentildealizacioacuten que participan en varios procesos con BMP-2 y
otras moleacuteculas (Padial-Molina Volk and Rios 2014) (Gamell et al 2008) Tambieacuten debe
mencionarse que el plazo de un ensayo de migracioacuten es corto Por lo tanto las ventajas potenciales
de un sistema de liberacioacuten controlada como el que se estaacute estudiando podriacutean ser limitadas Es
decir la liberacioacuten de BMP-2 de las nanopartiacuteculas como se demuestra en la Figura 27 se limita
a las primeras 48 h Por lo tanto se podriacutea hipotetizar un efecto positivo sostenido sobre la
actividad migratoria a lo largo del tiempo
119
Figura 28 Ensayo de migracioacuten Porcentaje de cierre del aacuterea rayada a las 24 y 48 h en
diferentes grupos y dosis
bullProliferacioacuten celular
La proliferacioacuten es otra de las actividades celulares requeridas para la regeneracioacuten de tejidos
Sin embargo esta propiedad debe equilibrarse con la migracioacuten y la diferenciacioacuten y no aumentar
las tres caracteriacutesticas al mismo tiempo y con las mismas proporciones (Friedrichs et al 2011)
De hecho seguacuten los informes cuando una dosis de BMP-2 induce una mayor proliferacioacuten
disminuye la diferenciacioacuten (Hrubi et al 2018) Esta propiedad ha sido ampliamente analizada
pero las discrepancias auacuten se pueden detectar en la literatura Por lo tanto Kim y colaboradores
analizoacute diferentes dosis de BMP-2 y su efecto sobre la proliferacioacuten celular y la apoptosis Se
confirmoacute in vitro que las dosis altas pero auacuten maacutes bajas que las utilizadas cliacutenicamente reducen
la proliferacioacuten celular y aumentan la apoptosis (Kim Oxendine and Kamiya 2013) Esto debe
ser evitado Hemos encontrado que aunque el BMP-2 libre no induce una proliferacioacuten mayor
que el control en ninguna de las dosis aplicadas ni en los puntos de tiempo (ANOVA con la prueba
0
10
20
30
40
50
60
70
80
90
100
0 h 24 h 48 h
o
f sc
ratc
hed
are
a
Time point
Control
BMP2-125 ngmL
BMP2-25 ngmL
BMP2-5 ngmL
NP-BMP2-125 ngmL
NP-BMP2-25 ngmL
NP-BMP2-5 ngmL
NP-BSA-BMP2-125 ngmL
NP-BSA-BMP2-25 ngmL
NP-BSA-BMP2-5 ngmL
d
e aacuter
ea r
ayad
a
Tiempo
120
de comparaciones muacuteltiples de Tukey) la misma cantidad de BMP-2 encapsulada o adsorbida en
nanopartiacuteculas de PLGA aumenta la proliferacioacuten siendo esto estadiacutesticamente significativo
cuando se usa una dosis de 25 ng mL o maacutes (ANOVA con prueba de comparaciones muacuteltiples
de Tukey) (Figura 29) Estas dosis son auacuten maacutes bajas que las sugeridas en estudios anteriores
Aparte de esa diferencia todaviacutea se logroacute un efecto positivo sobre la proliferacioacuten Ademaacutes
siguiendo el patroacuten de lanzamiento de la Figura 27 se espera que se libere maacutes BMP-2 con el
tiempo maacutes allaacute del marco de tiempo de 7 diacuteas Por lo tanto tambieacuten podriacutea esperarse un efecto
de induccioacuten sostenido hasta la confluencia completa del cultivo celular
Figura 29 Proliferacioacuten de ceacutelulas del estroma mesenquimatoso humano (MSC) medida por
absorbancia de sulforamida (SRB) Los resultados se normalizaron a T0 en cada grupo
T0 T1 T2 T3 T4 T6 T70
2
4
6
Time point
No
rmalized
Ab
so
rban
ce
NP-BSA-BMP2-125 ngmL
NP-BSA-BMP2-25 ngmL
NP-BSA-BMP2-5 ngmL
NP-BMP2-125 ngmL
NP-BMP2-25 ngmL
NP-BMP2-5 ngmL
BMP2-125 ngmL
BMP2-25 ngmL
BMP2-5 ngmL
Control
Abso
rban
cia
norm
aliz
ada
Tiempo
121
bullDiferenciacioacuten osteogeacutenica
Se ha confirmado que la diferenciacioacuten celular inducida por BMP-2 necesita la presencia de
componentes osteoinductores permisivos En particular se ha demostrado que el beta-
glicerofosfato ejerce un efecto sineacutergico con BMP-2 para inducir la diferenciacioacuten celular (Hrubi
et al 2018) Por lo tanto para probar la diferenciacioacuten osteogeacutenica analizamos la expresioacuten del
ARNm de ALP Se encontroacute que la actividad maacutexima de ALP se produce 10 diacuteas despueacutes de la
estimulacioacuten con micropartiacuteculas basadas en PLGA que contienen BMP-2 en coencapsulacioacuten
con albuacutemina seacuterica humana (Kirby et al 2011) Aunque otras pruebas podriacutean haberse utilizado
para reforzar nuestros hallazgos se sabe que ALP modula la deposicioacuten de noacutedulos mineralizados
lo que indica actividad osteoblaacutestica Para todo esto complementamos los medios de
diferenciacioacuten con Beta-glicerofosfato y BMP-2 NP-BMP2 o NP-BSA-BMP2 libres durante 4 y
7 diacuteas para poder capturar la dinaacutemica temprana de la expresioacuten del gen En nuestro estudio
identificamos un aumento en la expresioacuten de ALP en todos los grupos desde el diacutea 4 hasta el diacutea
7 (Figura 30) Aunque ALP en el diacutea 7 en el grupo BMP-2 parece ser maacutes alto que en los otros
dos grupos el cambio no resultoacute significativo De hecho las diferencias entre los grupos no fueron
estadiacutesticamente significativas en ninguacuten periacuteodo de tiempo Sin embargo cabe destacar que el
aumento no fue significativo dentro del grupo BMP-2 (p = 0141 prueba t de Student) pero fue
significativo dentro de los otros dos grupos (p = 0025 y p = 0003 NP-BMP2 y NP-BSA- Grupos
BMP2 respectivamente) Esto nuevamente podriacutea tomarse como una confirmacioacuten de la
liberacioacuten sostenida de la proteiacutena del sistema de nanopartiacuteculas maacutes allaacute de los puntos temporales
anteriores
Esto y los estudios de migracioacuten y proliferacioacuten descritos a continuacioacuten nos llevan a confirmar
que el sistema propuesto puede mantener una liberacioacuten adecuada de BMP-2 a lo largo del tiempo
manteniendo un efecto positivo en la migracioacuten y proliferacioacuten celular con dosis iniciales
reducidas de BMP-2 El hecho de que se evite el estallido inicial excesivo es importante para la
aplicacioacuten de esta nanotecnologiacutea en la regeneracioacuten oacutesea como en la odontologiacutea De esta
122
manera los efectos negativos de las altas dosis iniciales de BMP-2 se evitan al mismo tiempo que
la moleacutecula estaacute protegida contra la desnaturalizacioacuten dentro del NP Por lo tanto los efectos del
regenerador se mantienen con el tiempo Los experimentos in vitro mostraron que las NP de
PLGA cargadas con BMP-2 son los nanoportadores con el mejor perfil de liberacioacuten a corto plazo
sin una explosioacuten inicial y con una liberacioacuten moderada y sostenida de proteiacutena activa antes del
inicio de la degradacioacuten del poliacutemero Por lo tanto la actividad bioloacutegica es positiva sin
interaccioacuten negativa con la migracioacuten o la proliferacioacuten sino maacutes bien la induccioacuten de la
diferenciacioacuten celular a traveacutes de la expresioacuten de ALP
Figura 30 Cambio de pliegue relativo en la expresioacuten de ARNm de ALP (grupo de control
BMP2 a los 4 diacuteas) = Importancia estadiacutestica de la comparacioacuten a lo largo del tiempo (p =
0025 y p = 0003 prueba t del estudiante grupos NP-BMP2 y NP-BSA-BMP2)
Los experimentos de liberacioacuten in vitro muestran un patroacuten adecuado de administracioacuten a corto
plazo que al mismo tiempo preserva la bioactividad de la biomoleacutecula encapsulada Ademaacutes la
distribucioacuten de tamantildeo de nanopartiacutecula encontrada para este nanosistema W-F68 permite la
Tiempo
Cam
bio
de
pli
egue
123
posibilidad de una administracioacuten de proteiacutena dual externa e intracelular como se ha demostrado
mediante experimentos celulares in vitro Esta nueva formulacioacuten se utilizaraacute en futuros estudios
para encapsular y administrar factores de crecimiento in vitro e in vivo con el fin de explotar el
potencial terapeacuteutico de este nanosistema
Se ha puesto de manifiesto la necesidad de optimizacioacuten de los meacutetodos y componentes para
equilibrar la estructura y morfologiacutea de las micropartiacuteculas nanopartiacuteculas de PLGA logrando
de esta forma una alta eficiencia de encapsulacioacuten de BMP-2 y buscando un objetivo principal el
control de la entrega la reduccioacuten de la descarga inicial para alcanzar un perfil de liberacioacuten de
la proteiacutena sostenido en el tiempo preservando la actividad bioloacutegica y dirigieacutendola a ceacutelulas
diana para minimizar la cantidad cliacutenica de proteiacutena necesaria permitiendo al mismo tiempo una
correcta regeneracioacuten del tejido oacuteseo
En consecuencia otro reto futuro es conseguir el direccionamiento especiacutefico de estas nano-
esferas de PLGA cargadas de agentes activos Este aspecto se puede desarrollar mediante el uso
de unos ligandos que reconozcan especiacuteficamente los tipos o liacuteneas celulares a la que queremos
dirigir la liberacioacuten de biomoleacuteculas encapsuladas El uso de nanopartiacuteculas con una unioacuten
covalente de diferentes ligandos da lugar a una teacutecnica con un alto potencial de administracioacuten
que permite a la ingenieriacutea tisular un gran avance en cuanto a la distribucioacuten y administracioacuten de
diferentes faacutermacos o biomoleacuteculas mejorando asiacute las funciones bioloacutegicas o regenerativas
celulares
Los anticuerpos especiacuteficos que reconocen los receptores de superficie celular pueden unirse
covalentemente a la superficie de nuestras nanopartiacuteculas de PLGA dando lugar a una ldquoinmuno-
nanopartiacuteculardquo Para que esta unioacuten se produzca sin ninguacuten inconveniente en muchas ocasiones
hay que hacer uso de agentes estabilizadores para proporcionar estabilidad coloidal a las
nanopartiacuteculas sin que lleguen a afectar al enlace establecido entre los anticuerpos especiacuteficos de
los receptores celulares y las nanopartiacuteculas que es donde nos encontramos hoy diacutea y donde
124
muchos investigadores siguen haciendo avances cada diacutea entorno a la mejora de estos enlaces y
de estas entregas celulares especificas a traveacutes de las ldquoinmuno-nanopartiacuteculasrdquo
125
6CONCLUSIONES
En respuesta al objetivo principal de este trabajo
Los nanosistemas de partiacuteculas polimeacutericas basados en PLGA son sistemas prometedores para la
administracioacuten espacial y temporalmente controlada de factores de crecimiento que promueven
el desarrollo celular la diferenciacioacuten y regeneracioacuten en ingenieriacutea oacutesea mediante su
incorporacioacuten junto a las ceacutelulas en estructuras soacutelidas o hidrogeles
En respuesta a los objetivos secundarios de este trabajo
bullHa sido posible optimizar la obtencioacuten de diferentes sistemas de NPs de PLGA mediante un
procedimiento de doble emulsioacuten con las propiedades superficiales adecuadas que proporcionan
estabilidad coloidal y grupos carboxilo superficiales para unir quiacutemicamente diferentes ligandos
especiacuteficos en respuesta al objetivo 1
bullSe ha optimizado una formulacioacuten W-F68 que basada en el procedimiento anterior permite
encapsular moleacuteculas hidrofiacutelicas como las proteiacutenas obteniendo un novedoso nanotransportador
de tamantildeo dual que preserva la actividad bioloacutegica de la proteiacutena modelo encapsulada (lisozima)
en respuesta al objetivo 2
bullEn respuesta al tercer objetivo el anaacutelisis de la interaccioacuten proteiacutena-surfactante muestra el
papel crucial del solvente orgaacutenico el surfactante la relacioacuten de volumen entre ambas fases y la
carga neta de la proteiacutena encapsulada sobre las caracteriacutesticas finales de las NPs transportadoras
y el patroacuten de liberacioacuten proteica
bullLas experiencias in vitro de suministro proteico muestran una difusioacuten bien equilibrada de la
proteiacutena evitando una descarga inicial excesiva manteniendo un flujo constante de liberacioacuten a
corto plazo y permitiendo un suministro dual extra e intracelular sin citotoxicidad apreciable en
respuesta a los objetivos 4 y 5
126
bullSe ha desarrollado un nanosistema transportador de BMP2 sobre la base de un sistema modelo
formulado previamente mediante un procedimiento de doble emulsioacuten que conduce a un sistema
de NPs con una distribucioacuten dual de tamantildeos y estabilidad coloidal y temporal adecuada para
aplicaciones bioloacutegicas en respuesta al objetivo 6
bullIn vitro el Sistema con BMP2 encapsulada presenta un patroacuten de liberacioacuten en el corto plazo
7 diacuteas que muestra un suministro moderado y sostenido de proteiacutena bioloacutegicamente activa en
respuesta al objetivo 7
bullIn vitro la actividad bioloacutegica a nivel celular muestra mediante el anaacutelisis de la expresioacuten de
ALP la capacidad de la BMP2 nanotransportada para inducir diferenciacioacuten celular sin incidencia
negativa en los procesos de migracioacuten y proliferacioacuten celular en respuesta al objetivo 8
127
7 CONFLICTO DE INTERESES
Los autores declaran no tener ninguacuten conflicto de intereses con ninguno de los productos
enumerados en el documento
8 RECURSOS ECONOacuteMICOS
Beca de investigacioacuten obtenida competitivamente y otorgada por la empresa de implantes
dentales ldquoMIS IBERICA SLrdquo
Financiacioacuten parcial otorgada 1- por la Consejeriacutea de Economiacutea Innovacioacuten Educacioacuten
Ciencia y Empleo de la Junta de Andaluciacutea (Espantildea) 2- los proyectos MAT2013-43922-R ndash
incluyendo soporte europeo FEDER - (MICINN Espantildea) 3- y los Grupos de Investigacioacuten
FQM-115 CTS-1028 CTS-138 y CTS- 583 (Junta de Andaluciacutea Espantildea)
128
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Arias J L et al (2015) lsquoNanobody conjugated PLGA nanoparticles for active targeting of
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Balmayor E R et al (2009) lsquoStarch-poly-epsilon-caprolactone microparticles reduce the
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Baumann B et al (2017) lsquoControl of Nanoparticle Release Kinetics from 3D Printed
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Begam H et al (2017) lsquoStrategies for delivering bone morphogenetic protein for bone
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Bohr A et al (2015) lsquoPharmaceutical microparticle engineering with electrospraying the
role of mixed solvent systems in particle formation and characteristicsrsquo Journal of materials
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Bouissou C et al (2004) lsquoControlled release of the fibronectin central cell binding domain
from polymeric microspheresrsquo Journal of controlled release official journal of the
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Boyne P J et al (2005) lsquoDe novo bone induction by recombinant human bone
morphogenetic protein-2 (rhBMP-2) in maxillary sinus floor augmentationrsquo Journal of oral
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Boyne P and Jones S D (2004) lsquoDemonstration of the osseoinductive effect of bone
morphogenetic protein within endosseous dental implantsrsquo Implant dentistry 13(2) pp
180ndash4 doi 10109701id00001275200644342
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Brigger I Dubernet C and Couvreur P (2002) lsquoNanoparticles in cancer therapy and
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Brown B N et al (2009) lsquoMacrophage phenotype and remodeling outcomes in response to
biologic scaffolds with and without a cellular componentrsquo Biomaterials 30(8) pp 1482ndash
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Brown B N et al (2011) lsquoComparison of three methods for the derivation of a biologic
scaffold composed of adipose tissue extracellular matrixrsquo Tissue engineering Part C
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Bustos-Valenzuela J C et al (2011) lsquoUnveiling novel genes upregulated by both rhBMP2
and rhBMP7 during early osteoblastic transdifferentiation of C2C12 cellsrsquo BMC research
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Cai C et al (2008) lsquoCharged nanoparticles as protein delivery systems a feasibility study
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Carragee E J Hurwitz E L and Weiner B K (2011) lsquoA critical review of recombinant
human bone morphogenetic protein-2 trials in spinal surgery emerging safety concerns
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Carreira A C et al (2014) lsquoBone morphogenetic proteins facts challenges and future
perspectivesrsquo Journal of dental research 93(4) pp 335ndash345 doi
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Carreira Ana Claudia et al (2014) lsquoBone Morphogenetic Proteins structure biological
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Del Castillo-Santaella T et al (2019) lsquoInteraction of surfactant and protein at the OW
interface and its effect on colloidal and biological properties of polymeric nanocarriersrsquo
Colloids and surfaces B Biointerfaces 173 pp 295ndash302 doi
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Chan J M et al (2009) lsquoPLGA-lecithin-PEG core-shell nanoparticles for controlled drug
deliveryrsquo Biomaterials 30(8) pp 1627ndash1634 doi 101016jbiomaterials200812013
Chang H-C et al (2017) lsquoBone morphogenetic protein-2 loaded poly(DL-lactide-co-
glycolide) microspheres enhance osteogenic potential of gelatinhydroxyapatitebeta-
tricalcium phosphate cryogel composite for alveolar ridge augmentationrsquo Journal of the
Formosan Medical Association = Taiwan yi zhi 116(12) pp 973ndash981 doi
101016jjfma201701005
Chen G Deng C and Li Y-P (2012) lsquoTGF-beta and BMP signaling in osteoblast
differentiation and bone formationrsquo International journal of biological sciences 8(2) pp
272ndash288 doi 107150ijbs2929
Cheng J et al (2007) lsquoFormulation of functionalized PLGA-PEG nanoparticles for in vivo
targeted drug deliveryrsquo Biomaterials 28(5) pp 869ndash876 doi
101016jbiomaterials200609047
Chou L Y T Ming K and Chan W C W (2011) lsquoStrategies for the intracellular delivery of
nanoparticlesrsquo Chemical Society reviews 40(1) pp 233ndash245 doi 101039c0cs00003e
Chung V H-Y et al (2012) lsquoEngineered autologous bone marrow mesenchymal stem cells
alternative to cleft alveolar bone graft surgeryrsquo The Journal of craniofacial surgery 23(5)
pp 1558ndash1563 doi 101097SCS0b013e31825e4e30
Chung Y-I et al (2007) lsquoEnhanced bone regeneration with BMP-2 loaded functional
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nanoparticle-hydrogel complexrsquo Journal of controlled release official journal of the
Controlled Release Society 121(1ndash2) pp 91ndash99 doi 101016jjconrel200705029
Cleland J L (1997) lsquoProtein delivery from biodegradable microspheresrsquo Pharmaceutical
biotechnology 10 pp 1ndash43 doi 1010070-306-46803-4_1
Csaba N et al (2004) lsquoDesign and characterisation of new nanoparticulate polymer blends
for drug deliveryrsquo Journal of biomaterials science Polymer edition 15(9) pp 1137ndash1151
doi 1011631568562041753098
Csaba N et al (2005) lsquoPLGApoloxamer and PLGApoloxamine blend nanoparticles new
carriers for gene deliveryrsquo Biomacromolecules 6(1) pp 271ndash278 doi
101021bm049577p
Csaba N Garcia-Fuentes M and Alonso M J (2006) lsquoThe performance of nanocarriers for
transmucosal drug deliveryrsquo Expert opinion on drug delivery 3(4) pp 463ndash478 doi
1015171742524734463
drsquoAngelo I et al (2010) lsquoNanoparticles based on PLGApoloxamer blends for the delivery of
proangiogenic growth factorsrsquo Molecular pharmaceutics 7(5) pp 1724ndash1733 doi
101021mp1001262
Danhier F et al (2012) lsquoPLGA-based nanoparticles an overview of biomedical
applicationsrsquo Journal of controlled release official journal of the Controlled Release Society
161(2) pp 505ndash522 doi 101016jjconrel201201043
Deschaseaux F Sensebe L and Heymann D (2009) lsquoMechanisms of bone repair and
regenerationrsquo Trends in molecular medicine 15(9) pp 417ndash429 doi
101016jmolmed200907002
Devine J G et al (2012) lsquoThe use of rhBMP in spine surgery is there a cancer riskrsquo
Evidence-based spine-care journal 3(2) pp 35ndash41 doi 101055s-0031-1298616
Ding D and Zhu Q (2018) lsquoRecent advances of PLGA micronanoparticles for the delivery
133
of biomacromolecular therapeuticsrsquo Materials science amp engineering C Materials for
biological applications 92 pp 1041ndash1060 doi 101016jmsec201712036
Ertl B et al (2000) lsquoLectin-mediated bioadhesion preparation stability and caco-2 binding
of wheat germ agglutinin-functionalized Poly(DL-lactic-co-glycolic acid)-microspheresrsquo
Journal of drug targeting 8(3) pp 173ndash184 doi 10310910611860008996863
Fang D-L et al (2014) lsquoDevelopment of lipid-shell and polymer core nanoparticles with
water-soluble salidroside for anti-cancer therapyrsquo International journal of molecular
sciences 15(3) pp 3373ndash3388 doi 103390ijms15033373
Farace C et al (2016) lsquoImmune cell impact of three differently coated lipid nanocapsules
pluronic chitosan and polyethylene glycolrsquo Scientific reports 6 p 18423 doi
101038srep18423
Feczkoacute T Toacuteth J and Gyenis J (2008) lsquoComparison of the preparation of PLGA-BSA nano-
and microparticles by PVA poloxamer and PVPrsquo Colloids and Surfaces A Physicochemical
and Engineering Aspects 319(1ndash3) pp 188ndash195 doi 101016jcolsurfa200707011
Feng S and Huang G (2001) lsquoEffects of emulsifiers on the controlled release of paclitaxel
(Taxol) from nanospheres of biodegradable polymersrsquo Journal of controlled release official
journal of the Controlled Release Society 71(1) pp 53ndash69 doi 101016s0168-
3659(00)00364-3
Fraylich M et al (2008) lsquoPoly(DL-lactide-co-glycolide) dispersions containing pluronics
from particle preparation to temperature-triggered aggregationrsquo Langmuir the ACS
journal of surfaces and colloids 24(15) pp 7761ndash7768 doi 101021la800869u
Fredenberg S et al (2011) lsquoThe mechanisms of drug release in poly(lactic-co-glycolic acid)-
based drug delivery systems--a reviewrsquo International journal of pharmaceutics 415(1ndash2)
pp 34ndash52 doi 101016jijpharm201105049
Friedrichs M et al (2011) lsquoBMP signaling balances proliferation and differentiation of
134
muscle satellite cell descendantsrsquo BMC cell biology 12 p 26 doi 1011861471-2121-12-
26
Froum S J et al (2006) lsquoComparison of mineralized cancellous bone allograft (Puros) and
anorganic bovine bone matrix (Bio-Oss) for sinus augmentation histomorphometry at 26
to 32 weeks after graftingrsquo The International journal of periodontics amp restorative dentistry
26(6) pp 543ndash551
Fu C et al (2017) lsquoEnhancing Cell Proliferation and Osteogenic Differentiation of MC3T3-
E1 Pre-osteoblasts by BMP-2 Delivery in Graphene Oxide-Incorporated PLGAHA
Biodegradable Microcarriersrsquo Scientific reports 7(1) p 12549 doi 101038s41598-017-
12935-x
Fu R et al (2013) lsquoEffectiveness and harms of recombinant human bone morphogenetic
protein-2 in spine fusion a systematic review and meta-analysisrsquo Annals of internal
medicine 158(12) pp 890ndash902 doi 1073260003-4819-158-12-201306180-00006
Fu Y et al (2012) lsquoIn vitro sustained release of recombinant human bone morphogenetic
protein-2 microspheres embedded in thermosensitive hydrogelsrsquo Die Pharmazie 67(4) pp
299ndash303
Galindo-Moreno P et al (2007) lsquoEvaluation of sinus floor elevation using a composite bone
graft mixturersquo Clinical oral implants research 18(3) pp 376ndash382 doi 101111j1600-
0501200701337x
Galindo-Moreno P et al (2011) lsquoEffect of anorganic bovine bone to autogenous cortical
bone ratio upon bone remodeling patterns following maxillary sinus augmentationrsquo Clinical
oral implants research 22(8) pp 857ndash864 doi 101111j1600-0501201002073x
Gamell C et al (2008) lsquoBMP2 induction of actin cytoskeleton reorganization and cell
migration requires PI3-kinase and Cdc42 activityrsquo Journal of cell science 121(Pt 23) pp
3960ndash3970 doi 101242jcs031286
135
Gaudana R et al (2013) lsquoDesign and evaluation of a novel nanoparticulate-based
formulation encapsulating a HIP complex of lysozymersquo Pharmaceutical development and
technology 18(3) pp 752ndash759 doi 103109108374502012737806
Ghaderi R and Carlfors J (1997) lsquoBiological activity of lysozyme after entrapment in
poly(dl-lactide-co-glycolide)-microspheresrsquo Pharmaceutical research 14(11) pp 1556ndash
1562 doi 101023a1012122200381
Giteau A et al (2008) lsquoHow to achieve sustained and complete protein release from PLGA-
based microparticlesrsquo International journal of pharmaceutics 350(1ndash2) pp 14ndash26 doi
101016jijpharm200711012
Gref R et al (1994) lsquoBiodegradable long-circulating polymeric nanospheresrsquo Science (New
York NY) 263(5153) pp 1600ndash1603 doi 101126science8128245
Hans M L and Lowman A M (2002) lsquoBiodegradable nanoparticles for drug delivery and
targetingrsquo Current Opinion in Solid State and Materials Science 6(4) pp 319ndash327 doi
101016S1359-0286(02)00117-1
Hassan P A Rana S and Verma G (2015) lsquoMaking sense of Brownian motion colloid
characterization by dynamic light scatteringrsquo Langmuir the ACS journal of surfaces and
colloids 31(1) pp 3ndash12 doi 101021la501789z
Hines D J and Kaplan D L (2013) lsquoPoly(lactic-co-glycolic) acid-controlled-release systems
experimental and modeling insightsrsquo Critical reviews in therapeutic drug carrier systems
30(3) pp 257ndash276 doi 101615critrevtherdrugcarriersyst2013006475
Hong P et al (2013) lsquoEnhancement of bone consolidation in mandibular distraction
osteogenesis a contemporary review of experimental studies involving adjuvant
therapiesrsquo Journal of plastic reconstructive amp aesthetic surgery JPRAS 66(7) pp 883ndash895
doi 101016jbjps201303030
Houghton P et al (2007) lsquoThe sulphorhodamine (SRB) assay and other approaches to
136
testing plant extracts and derived compounds for activities related to reputed anticancer
activityrsquo Methods (San Diego Calif) 42(4) pp 377ndash387 doi 101016jymeth200701003
Hrubi E et al (2018) lsquoDiverse effect of BMP-2 homodimer on mesenchymal progenitors of
different originrsquo Human cell 31(2) pp 139ndash148 doi 101007s13577-018-0202-5
Inai K et al (2008) lsquoBMP-2 induces cell migration and periostin expression during
atrioventricular valvulogenesisrsquo Developmental biology 315(2) pp 383ndash396 doi
101016jydbio200712028
Iqbal M et al (2015) lsquoDouble emulsion solvent evaporation techniques used for drug
encapsulationrsquo International journal of pharmaceutics 496(2) pp 173ndash190 doi
101016jijpharm201510057
Jana Sougata and Jana Subrata (2017) lsquoNatural polymeric biodegradable nanoblend for
macromolecules deliveryrsquo in Recent Developments in Polymer Macro Micro and Nano Blends
Preparation and Characterisation Elsevier Inc pp 289ndash312 doi 101016B978-0-08-
100408-100010-8
Jeon O et al (2008) lsquoLong-term delivery enhances in vivo osteogenic efficacy of bone
morphogenetic protein-2 compared to short-term deliveryrsquo Biochemical and biophysical
research communications 369(2) pp 774ndash780 doi 101016jbbrc200802099
Ji W et al (2012) lsquoLocal delivery of small and large biomolecules in craniomaxillofacial
bonersquo Advanced drug delivery reviews 64(12) pp 1152ndash1164 doi
101016jaddr201203003
Ji Y et al (2010) lsquoBMP-2PLGA delayed-release microspheres composite graft selection of
bone particulate diameters and prevention of aseptic inflammation for bone tissue
engineeringrsquo Annals of biomedical engineering 38(3) pp 632ndash639 doi 101007s10439-
009-9888-6
Jiang W et al (2005) lsquoBiodegradable poly(lactic-co-glycolic acid) microparticles for
137
injectable delivery of vaccine antigensrsquo Advanced drug delivery reviews 57(3) pp 391ndash410
doi 101016jaddr200409003
Kao D W K et al (2012) lsquoThe negative effect of combining rhBMP-2 and Bio-Oss on bone
formation for maxillary sinus augmentationrsquo The International journal of periodontics amp
restorative dentistry 32(1) pp 61ndash67
Katranji A Fotek P and Wang H-L (2008) lsquoSinus augmentation complications etiology
and treatmentrsquo Implant dentistry 17(3) pp 339ndash349 doi
101097ID0b013e3181815660
Kempen D H R et al (2008) lsquoRetention of in vitro and in vivo BMP-2 bioactivities in
sustained delivery vehicles for bone tissue engineeringrsquo Biomaterials 29(22) pp 3245ndash
3252 doi 101016jbiomaterials200804031
Kempen D H R et al (2009) lsquoEffect of local sequential VEGF and BMP-2 delivery on ectopic
and orthotopic bone regenerationrsquo Biomaterials 30(14) pp 2816ndash2825 doi
101016jbiomaterials200901031
Ki-Bum Lee Ani Solanki J Dongun Kim J J (2009) Nanomedicine Dynamic Integration of
Nanotechnology with Biomedical Science | Request PDF Available at
httpswwwresearchgatenetpublication254745458_Nanomedicine_Dynamic_Integrati
on_of_Nanotechnology_with_Biomedical_Science (Accessed 29 March 2020)
Kim H K W Oxendine I and Kamiya N (2013) lsquoHigh-concentration of BMP2 reduces cell
proliferation and increases apoptosis via DKK1 and SOST in human primary periosteal
cellsrsquo Bone 54(1) pp 141ndash150 doi 101016jbone201301031
Kim Y-H and Tabata Y (2015) lsquoDual-controlled release system of drugs for bone
regenerationrsquo Advanced drug delivery reviews 94 pp 28ndash40 doi
101016jaddr201506003
Kirby G T S et al (2011) lsquoPLGA-based microparticles for the sustained release of BMP-2rsquo
138
in European Cells and Materials AO Research Institute Davos p 24 doi
103390polym3010571
Kocbek P et al (2007) lsquoTargeting cancer cells using PLGA nanoparticles surface modified
with monoclonal antibodyrsquo Journal of controlled release official journal of the Controlled
Release Society 120(1ndash2) pp 18ndash26 doi 101016jjconrel200703012
Kok R J et al (1998) lsquoDrug delivery to the kidneys and the bladder with the low molecular
weight protein lysozymersquo Renal failure 20(2) pp 211ndash217 doi
10310908860229809045104
Kumar B et al (2017) lsquoRecent advances in nanoparticle-mediated drug deliveryrsquo Journal of
Drug Delivery Science and Technology Editions de Sante pp 260ndash268 doi
101016jjddst201707019
Kumar T R S Soppimath K and Nachaegari S K (2006) lsquoNovel delivery technologies for
protein and peptide therapeuticsrsquo Current pharmaceutical biotechnology 7(4) pp 261ndash
276 doi 102174138920106777950852
Kumari A Yadav S K and Yadav S C (2010) lsquoBiodegradable polymeric nanoparticles
based drug delivery systemsrsquo Colloids and surfaces B Biointerfaces 75(1) pp 1ndash18 doi
101016jcolsurfb200909001
La W-G et al (2010) lsquoThe efficacy of bone morphogenetic protein-2 depends on its mode
of deliveryrsquo Artificial organs 34(12) pp 1150ndash1153 doi 101111j1525-
1594200900988x
Lee J et al (2013) lsquoSinus augmentation using rhBMP-2ACS in a mini-pig model relative
efficacy of autogenous fresh particulate iliac bone graftsrsquo Clinical oral implants research
24(5) pp 497ndash504 doi 101111j1600-0501201102419x
Lee S-J et al (2017) lsquoDevelopment of Novel 3-D Printed Scaffolds With Core-Shell
Nanoparticles for Nerve Regenerationrsquo IEEE transactions on bio-medical engineering 64(2)
139
pp 408ndash418 doi 101109TBME20162558493
Li B et al (2009) lsquoThe effects of rhBMP-2 released from biodegradable
polyurethanemicrosphere composite scaffolds on new bone formation in rat femorarsquo
Biomaterials 30(35) pp 6768ndash6779 doi 101016jbiomaterials200908038
Liang C-C Park A Y and Guan J-L (2007) lsquoIn vitro scratch assay a convenient and
inexpensive method for analysis of cell migration in vitrorsquo Nature protocols 2(2) pp 329ndash
333 doi 101038nprot200730
LIN Y et al (2007) lsquoIn vitro Evaluation of Lysozyme-loaded Microspheres in
Thermosensitive Methylcellulose-based Hydrogel1 1 Supported by the National Natural
Science Foundation of China (No20576057) and Fundamental Research Foundation of
Tsinghua University (JCqn2005033)rsquo Chinese Journal of Chemical Engineering 15(4) pp
566ndash572 doi 101016S1004-9541(07)60125-6
Lochmann A et al (2010) lsquoThe influence of covalently linked and free polyethylene glycol
on the structural and release properties of rhBMP-2 loaded microspheresrsquo Journal of
controlled release official journal of the Controlled Release Society 147(1) pp 92ndash100 doi
101016jjconrel201006021
Loureiro J A et al (2016) lsquoCellular uptake of PLGA nanoparticles targeted with anti-amyloid
and anti-transferrin receptor antibodies for Alzheimerrsquos disease treatmentrsquo Colloids and
surfaces B Biointerfaces 145 pp 8ndash13 doi 101016jcolsurfb201604041
Luginbuehl V et al (2004) lsquoLocalized delivery of growth factors for bone repairrsquo European
journal of pharmaceutics and biopharmaceutics official journal of Arbeitsgemeinschaft fur
Pharmazeutische Verfahrenstechnik eV 58(2) pp 197ndash208 doi
101016jejpb200403004
M Padial-Molina P Galindo-Moreno G Aacute-M (2009) lsquoBiomimetic ceramics in implant
dentistryrsquo MINERVA BIOTECNOLOGICA 21 p 173
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Makadia H K and Siegel S J (2011) lsquoPoly Lactic-co-Glycolic Acid (PLGA) as Biodegradable
Controlled Drug Delivery Carrierrsquo Polymers 3(3) pp 1377ndash1397 doi
103390polym3031377
Maldonado-Valderrama J et al (2013) lsquoIn vitro digestion of interfacial protein structuresrsquo
Soft Matter 9(4) pp 1043ndash1053 doi 101039c2sm26843d
Mason S et al (2014) lsquoStandardization and safety of alveolar bone-derived stem cell
isolationrsquo Journal of dental research 93(1) pp 55ndash61 doi 1011770022034513510530
McClements D J (2018) lsquoEncapsulation protection and delivery of bioactive proteins and
peptides using nanoparticle and microparticle systems A reviewrsquo Advances in colloid and
interface science 253 pp 1ndash22 doi 101016jcis201802002
McKay W F Peckham S M and Badura J M (2007) lsquoA comprehensive clinical review of
recombinant human bone morphogenetic protein-2 (INFUSE Bone Graft)rsquo International
orthopaedics 31(6) pp 729ndash734 doi 101007s00264-007-0418-6
Meinel L et al (2001) lsquoStabilizing insulin-like growth factor-I in poly(DL-lactide-co-
glycolide) microspheresrsquo Journal of controlled release official journal of the Controlled
Release Society 70(1ndash2) pp 193ndash202 doi 101016s0168-3659(00)00352-7
Meng F T et al (2003) lsquoWOW double emulsion technique using ethyl acetate as organic
solvent effects of its diffusion rate on the characteristics of microparticlesrsquo Journal of
controlled release official journal of the Controlled Release Society 91(3) pp 407ndash416 doi
101016s0168-3659(03)00273-6
Mir M Ahmed N and Rehman A U (2017) lsquoRecent applications of PLGA based
nanostructures in drug deliveryrsquo Colloids and surfaces B Biointerfaces 159 pp 217ndash231
doi 101016jcolsurfb201707038
Misch C E (1987) lsquoMaxillary sinus augmentation for endosteal implants organized
alternative treatment plansrsquo The International journal of oral implantology implantologist
141
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Mohamed F and van der Walle C F (2008) lsquoEngineering biodegradable polyester particles
with specific drug targeting and drug release propertiesrsquo Journal of pharmaceutical sciences
97(1) pp 71ndash87 doi 101002jps21082
Morille M et al (2013) lsquoNew PLGA-P188-PLGA matrix enhances TGF-beta3 release from
pharmacologically active microcarriers and promotes chondrogenesis of mesenchymal
stem cellsrsquo Journal of controlled release official journal of the Controlled Release Society
170(1) pp 99ndash110 doi 101016jjconrel201304017
Mueller T D and Nickel J (2012) lsquoPromiscuity and specificity in BMP receptor activationrsquo
FEBS letters 586(14) pp 1846ndash1859 doi 101016jfebslet201202043
Myeroff C and Archdeacon M (2011) lsquoAutogenous bone graft donor sites and techniquesrsquo
The Journal of bone and joint surgery American volume 93(23) pp 2227ndash2236 doi
102106JBJSJ01513
Nair B P and Sharma C P (2012) lsquoPoly(lactide-co-glycolide)-laponite-F68 nanocomposite
vesicles through a single-step double-emulsion method for the controlled release of
doxorubicinrsquo Langmuir the ACS journal of surfaces and colloids 28(9) pp 4559ndash4564 doi
101021la300005c
Nevins M et al (1996) lsquoBone formation in the goat maxillary sinus induced by absorbable
collagen sponge implants impregnated with recombinant human bone morphogenetic
protein-2rsquo The International journal of periodontics amp restorative dentistry 16(1) pp 8ndash19
Oh S H Kim T H and Lee J H (2011) lsquoCreating growth factor gradients in three
dimensional porous matrix by centrifugation and surface immobilizationrsquo Biomaterials
32(32) pp 8254ndash8260 doi 101016jbiomaterials201107027
Ortega-Oller I et al (2015) lsquoBone Regeneration from PLGA Micro-Nanoparticlesrsquo BioMed
research international 2015 p 415289 doi 1011552015415289
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Ortega-Oller I et al (2017) lsquoDual delivery nanosystem for biomolecules Formulation
characterization and in vitro releasersquo Colloids and surfaces B Biointerfaces 159 pp 586ndash
595 doi 101016jcolsurfb201708027
Padial-Molina M et al (2012) lsquoMethods to validate tooth-supporting regenerative
therapiesrsquo Methods in molecular biology (Clifton NJ) 887 pp 135ndash148 doi 101007978-
1-61779-860-3_13
Padial-Molina M OrsquoValle F et al (2015) lsquoClinical Application of Mesenchymal Stem Cells
and Novel Supportive Therapies for Oral Bone Regenerationrsquo BioMed research
international 2015 p 341327 doi 1011552015341327
Padial-Molina M Rodriguez J C et al (2015) lsquoStandardized in vivo model for studying
novel regenerative approaches for multitissue bone-ligament interfacesrsquo Nature protocols
10(7) pp 1038ndash1049 doi 101038nprot2015063
Padial-Molina M et al (2019) lsquoExpression of Musashi-1 During Osteogenic Differentiation
of Oral MSC An In Vitro Studyrsquo International journal of molecular sciences 20(9) doi
103390ijms20092171
Padial-Molina M and Rios H F (2014) lsquoStem Cells Scaffolds and Gene Therapy for
Periodontal Engineeringrsquo Current Oral Health Reports 1(1) pp 16ndash25 doi
101007s40496-013-0002-7
Padial-Molina M Volk S L and Rios H F (2014) lsquoPeriostin increases migration and
proliferation of human periodontal ligament fibroblasts challenged by tumor necrosis factor
-alpha and Porphyromonas gingivalis lipopolysaccharidesrsquo Journal of periodontal research
49(3) pp 405ndash414 doi 101111jre12120
Paillard-Giteau A et al (2010) lsquoEffect of various additives and polymers on lysozyme
release from PLGA microspheres prepared by an sow emulsion techniquersquo European
journal of pharmaceutics and biopharmaceutics official journal of Arbeitsgemeinschaft fur
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Pharmazeutische Verfahrenstechnik eV 75(2) pp 128ndash136 doi
101016jejpb201003005
Pakulska M M et al (2016) lsquoEncapsulation-free controlled release Electrostatic adsorption
eliminates the need for protein encapsulation in PLGA nanoparticlesrsquo Science advances
2(5) p e1600519 doi 101126sciadv1600519
Pantazis P et al (2012) lsquoPreparation of siRNA-encapsulated PLGA nanoparticles for
sustained release of siRNA and evaluation of encapsulation efficiencyrsquo Methods in molecular
biology (Clifton NJ) 906 pp 311ndash319 doi 101007978-1-61779-953-2_25
Panyam J and Labhasetwar V (2003) lsquoDynamics of endocytosis and exocytosis of poly(DL-
lactide-co-glycolide) nanoparticles in vascular smooth muscle cellsrsquo Pharmaceutical
research 20(2) pp 212ndash220 doi 101023a1022219003551
Paolicelli P et al (2010) lsquoSurface-modified PLGA-based nanoparticles that can efficiently
associate and deliver virus-like particlesrsquo Nanomedicine (London England) 5(6) pp 843ndash
853 doi 102217nnm1069
Park J S et al (2013) lsquoMultilineage differentiation of human-derived dermal fibroblasts
transfected with genes coated on PLGA nanoparticles plus growth factorsrsquo Biomaterials
34(2) pp 582ndash597 doi 101016jbiomaterials201210001
Penaloza J P et al (2017) lsquoIntracellular trafficking and cellular uptake mechanism of PHBV
nanoparticles for targeted delivery in epithelial cell linesrsquo Journal of nanobiotechnology
15(1) p 1 doi 101186s12951-016-0241-6
Perez C De Jesus P and Griebenow K (2002) lsquoPreservation of lysozyme structure and
function upon encapsulation and release from poly(lactic-co-glycolic) acid microspheres
prepared by the water-in-oil-in-water methodrsquo International journal of pharmaceutics
248(1ndash2) pp 193ndash206 doi 101016s0378-5173(02)00435-0
Peula-Garcia J M Hidaldo-Alvarez R and De las Nieves F J (1997) lsquoProtein co-adsorption
144
on different polystyrene latexes Electrokinetic characterization and colloidal stabilityrsquo
Colloid and Polymer Science 275(2) pp 198ndash202 doi 101007s003960050072
Peula-Garciacutea J M Hidalgo-Alvarez R and De Las Nieves F J (1997) lsquoColloid stability and
electrokinetic characterization of polymer colloids prepared by different methodsrsquo Colloids
and Surfaces A Physicochemical and Engineering Aspects 127(1ndash3) pp 19ndash24 doi
101016S0927-7757(96)03890-3
Peula JM Callejas J de las Nieves F J (1994) lsquoAdsorption of Monomeric Bovine Serum
Albumin on Sulfonated Polystyrene Model Colloids II Electrokinetic Characterization of
Latex-Protein Complexesrsquo Surface Properties of Biomaterials pp 61ndash69
Peula J M Hidalgo-Alvarez R and de las Nieves F J (1995) lsquoCoadsorption of IgG and BSA
onto sulfonated polystyrene latex I Sequential and competitive coadsorption isothermsrsquo
Journal of biomaterials science Polymer edition 7(3) pp 231ndash240 doi
101163156856295x00274
Peula J M and de las Nieves F J (1993) lsquoAdsorption of monomeric bovine serum albumin
on sulfonated polystyrene model colloids 1 Adsorption isotherms and effect of the surface
charge densityrsquo Colloids and Surfaces A Physicochemical and Engineering Aspects 77(3) pp
199ndash208 doi 1010160927-7757(93)80117-W
Peula J M and de las Nieves F J (1994) lsquoAdsorption of monomeric bovine serum albumin
on sulfonated polystyrene model colloids 3 Colloidal stability of latex-protein complexesrsquo
Colloids and Surfaces A Physicochemical and Engineering Aspects 90(1) pp 55ndash62 doi
1010160927-7757(94)02889-3
Pezennec S et al (2008) The protein net electric charge determines the surface rheological
properties of ovalbumin adsorbed at the air-water interface Available at
wwwelseviercomlocatefoodhyd (Accessed 29 March 2020)
Pirooznia N et al (2012) lsquoEncapsulation of alpha-1 antitrypsin in PLGA nanoparticles in
145
vitro characterization as an effective aerosol formulation in pulmonary diseasesrsquo Journal of
nanobiotechnology 10 p 20 doi 1011861477-3155-10-20
Poinern G E J (no date) A laboratory course in nanoscience and nanotechnology
Puppi D et al (2014) lsquoNanomicrofibrous polymeric constructs loaded with bioactive
agents and designed for tissue engineering applications a reviewrsquo Journal of biomedical
materials research Part B Applied biomaterials 102(7) pp 1562ndash1579 doi
101002jbmb33144
Qutachi O Shakesheff K M and Buttery L D K (2013) lsquoDelivery of definable number of
drug or growth factor loaded poly(DL-lactic acid-co-glycolic acid) microparticles within
human embryonic stem cell derived aggregatesrsquo Journal of controlled release official
journal of the Controlled Release Society 168(1) pp 18ndash27 doi
101016jjconrel201302029
Rafati A et al (2012) lsquoChemical and spatial analysis of protein loaded PLGA microspheres
for drug delivery applicationsrsquo Journal of controlled release official journal of the Controlled
Release Society 162(2) pp 321ndash329 doi 101016jjconrel201205008
Rahman C V et al (2014) lsquoControlled release of BMP-2 from a sintered polymer scaffold
enhances bone repair in a mouse calvarial defect modelrsquo Journal of tissue engineering and
regenerative medicine 8(1) pp 59ndash66 doi 101002term1497
Ramel M-C and Hill C S (2012) lsquoSpatial regulation of BMP activityrsquo FEBS letters 586(14)
pp 1929ndash1941 doi 101016jfebslet201202035
Ratzinger G et al (2010) lsquoSurface modification of PLGA particles the interplay between
stabilizer ligand size and hydrophobic interactionsrsquo Langmuir the ACS journal of surfaces
and colloids 26(3) pp 1855ndash1859 doi 101021la902602z
Rescignano N et al (2016) lsquoIn-vitro degradation of PLGA nanoparticles in aqueous medium
and in stem cell cultures by monitoring the cargo fluorescence spectrumrsquo Polymer
146
Degradation and Stability 134 pp 296ndash304 doi 101016jpolymdegradstab201610017
van Rijt S and Habibovic P (2017) lsquoEnhancing regenerative approaches with
nanoparticlesrsquo Journal of the Royal Society Interface 14(129) doi 101098rsif20170093
Romagnoli C DrsquoAsta F and Brandi M L (2013) lsquoDrug delivery using composite scaffolds
in the context of bone tissue engineeringrsquo Clinical cases in mineral and bone metabolism
the official journal of the Italian Society of Osteoporosis Mineral Metabolism and Skeletal
Diseases 10(3) pp 155ndash161
Ronga M et al (2013) lsquoClinical applications of growth factors in bone injuries experience
with BMPsrsquo Injury 44 Suppl 1 pp S34-9 doi 101016S0020-1383(13)70008-1
Rosca I D Watari F and Uo M (2004) lsquoMicroparticle formation and its mechanism in
single and double emulsion solvent evaporationrsquo Journal of controlled release official
journal of the Controlled Release Society 99(2) pp 271ndash280 doi
101016jjconrel200407007
Sanchez-Moreno P et al (2013) lsquoSynthesis and characterization of lipid immuno-
nanocapsules for directed drug delivery selective antitumor activity against HER2 positive
breast-cancer cellsrsquo Biomacromolecules 14(12) pp 4248ndash4259 doi 101021bm401103t
Santander-Ortega M J et al (2006) lsquoColloidal stability of pluronic F68-coated PLGA
nanoparticles a variety of stabilisation mechanismsrsquo Journal of colloid and interface science
302(2) pp 522ndash529 doi 101016jjcis200607031
Santander-Ortega M J et al (2009) lsquoInsulin-loaded PLGA nanoparticles for oral
administration an in vitro physico-chemical characterizationrsquo Journal of biomedical
nanotechnology 5(1) pp 45ndash53 doi 101166jbn2009022
Santander-Ortega M J et al (2010) lsquoNanoparticles made from novel starch derivatives for
transdermal drug deliveryrsquo Journal of controlled release official journal of the Controlled
Release Society 141(1) pp 85ndash92 doi 101016jjconrel200908012
147
Santander-Ortega Manuel J Lozano-Loacutepez M V et al (2010) lsquoNovel core-shell lipid-
chitosan and lipid-poloxamer nanocapsules Stability by hydration forcesrsquo Colloid and
Polymer Science 288(2) pp 159ndash172 doi 101007s00396-009-2132-y
Santander-Ortega Manuel J Csaba N et al (2010) lsquoProtein-loaded PLGA-PEO blend
nanoparticles Encapsulation release and degradation characteristicsrsquo Colloid and Polymer
Science 288(2) pp 141ndash150 doi 101007s00396-009-2131-z
Santander-Ortega M J et al (2011) lsquoChitosan nanocapsules Effect of chitosan molecular
weight and acetylation degree on electrokinetic behaviour and colloidal stabilityrsquo Colloids
and surfaces B Biointerfaces 82(2) pp 571ndash580 doi 101016jcolsurfb201010019
Santander-Ortega M J Bastos-Gonzalez D and Ortega-Vinuesa J L (2007)
lsquoElectrophoretic mobility and colloidal stability of PLGA particles coated with IgGrsquo Colloids
and surfaces B Biointerfaces 60(1) pp 80ndash88 doi 101016jcolsurfb200706002
Santo V E et al (2012) lsquoFrom nano- to macro-scale nanotechnology approaches for
spatially controlled delivery of bioactive factors for bone and cartilage engineeringrsquo
Nanomedicine (London England) 7(7) pp 1045ndash1066 doi 102217nnm1278
Sapkota G et al (2007) lsquoBalancing BMP signaling through integrated inputs into the Smad1
linkerrsquo Molecular cell 25(3) pp 441ndash454 doi 101016jmolcel200701006
Schrier J A et al (2001) lsquoEffect of a freeze-dried CMCPLGA microsphere matrix of rhBMP-
2 on bone healingrsquo AAPS PharmSciTech 2(3) p E18 doi 101208pt020318
Schrier J A and DeLuca P P (2001) lsquoPorous bone morphogenetic protein-2 microspheres
polymer binding and in vitro releasersquo AAPS PharmSciTech 2(3) p E17 doi
101208pt020317
Schwendeman S P et al (2014) lsquoInjectable controlled release depots for large moleculesrsquo
Journal of controlled release official journal of the Controlled Release Society 190 pp 240ndash
253 doi 101016jjconrel201405057
148
Shankarayan R Kumar S and Mishra P (2013) lsquoDifferential permeation of piroxicam-
loaded PLGA micronanoparticles and their in vitro enhancementrsquo Journal of Nanoparticle
Research 15(3) doi 101007s11051-013-1496-6
Shim Y B et al (2016) lsquoFabrication of hollow porous PLGA microspheres using sucrose for
controlled dual delivery of dexamethasone and BMP2rsquo Journal of Industrial and Engineering
Chemistry 37 pp 101ndash106 doi 101016jjiec201603014
Siafaka P I et al (2016) lsquoSurface Modified Multifunctional and Stimuli Responsive
Nanoparticles for Drug Targeting Current Status and Usesrsquo International journal of
molecular sciences 17(9) doi 103390ijms17091440
Sieber C et al (2009) lsquoRecent advances in BMP receptor signalingrsquo Cytokine amp growth factor
reviews 20(5ndash6) pp 343ndash355 doi 101016jcytogfr200910007
Silva G A et al (2007) lsquoMaterials in particulate form for tissue engineering 2 Applications
in bonersquo Journal of tissue engineering and regenerative medicine 1(2) pp 97ndash109 doi
101002term1
Simmonds M C et al (2013) lsquoSafety and effectiveness of recombinant human bone
morphogenetic protein-2 for spinal fusion a meta-analysis of individual-participant datarsquo
Annals of internal medicine 158(12) pp 877ndash889 doi 1073260003-4819-158-12-
201306180-00005
Sneh-Edri H Likhtenshtein D and Stepensky D (2011) lsquoIntracellular targeting of PLGA
nanoparticles encapsulating antigenic peptide to the endoplasmic reticulum of dendritic
cells and its effect on antigen cross-presentation in vitrorsquo Molecular pharmaceutics 8(4)
pp 1266ndash1275 doi 101021mp200198c
Spagnoli D B and Marx R E (2011) lsquoDental implants and the use of rhBMP-2rsquo Dental clinics
of North America 55(4) pp 883ndash907 doi 101016jcden201107014
Srinivasan C et al (2005) lsquoEffect of additives on encapsulation efficiency stability and
149
bioactivity of entrapped lysozyme from biodegradable polymer particlesrsquo Journal of
microencapsulation 22(2) pp 127ndash138 doi 10108002652040400026400
Sturesson C and Carlfors J (2000) lsquoIncorporation of protein in PLG-microspheres with
retention of bioactivityrsquo Journal of controlled release official journal of the Controlled
Release Society 67(2ndash3) pp 171ndash178 doi 101016s0168-3659(00)00205-4
Sun D (2016) lsquoEffect of Zeta Potential and Particle Size on the Stability of SiO2 Nanospheres
as Carrier for Ultrasound Imaging Contrast Agentsrsquo Int J Electrochem Sci pp 8520ndash8529
Tan J S et al (1993) lsquoSurface modification of nanoparticles by PEOPPO block copolymers
to minimize interactions with blood components and prolong blood circulation in ratsrsquo
Biomaterials 14(11) pp 823ndash833 doi 1010160142-9612(93)90004-l
Tian Z et al (2012) lsquoSynthesis and characterization of UPPE-PLGA-rhBMP2 scaffolds for
bone regenerationrsquo Journal of Huazhong University of Science and Technology Medical
sciences = Hua zhong ke ji da xue xue bao Yi xue Ying De wen ban = Huazhong keji daxue
xuebao Yixue Yingdewen ban 32(4) pp 563ndash570 doi 101007s11596-012-0097-4
Torcello-Goacutemez A et al (2011) lsquoAdsorption of antibody onto Pluronic F68-covered
nanoparticles Link with surface propertiesrsquo Soft Matter 7(18) pp 8450ndash8461 doi
101039c1sm05570d
Torrecillas-Martinez L et al (2013) lsquoEffect of rhBMP-2 upon maxillary sinus augmentation
a comprehensive reviewrsquo Implant dentistry 22(3) pp 232ndash237 doi
101097ID0b013e31829262a8
Tran M-K Swed A and Boury F (2012) lsquoPreparation of polymeric particles in CO(2)
medium using non-toxic solvents formulation and comparisons with a phase separation
methodrsquo European journal of pharmaceutics and biopharmaceutics official journal of
Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik eV 82(3) pp 498ndash507 doi
101016jejpb201208005
150
Tsuji K et al (2006) lsquoBMP2 activity although dispensable for bone formation is required
for the initiation of fracture healingrsquo Nature genetics 38(12) pp 1424ndash1429 doi
101038ng1916
Tsuji K et al (2008) lsquoBMP4 is dispensable for skeletogenesis and fracture-healing in the
limbrsquo The Journal of bone and joint surgery American volume 90 Suppl 1 pp 14ndash18 doi
102106JBJSG01109
Tsuji K et al (2010) lsquoConditional deletion of BMP7 from the limb skeleton does not affect
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pp 893ndash899 doi 101126science1503698893
Vasir J K and Labhasetwar V (2007) lsquoBiodegradable nanoparticles for cytosolic delivery
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101016jaddr200706003
Vo T N Kasper F K and Mikos A G (2012) lsquoStrategies for controlled delivery of growth
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1309 doi 101016jaddr201201016
Wallace S S and Froum S J (2003) lsquoEffect of maxillary sinus augmentation on the survival
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328ndash343 doi 101902annals200381328
Wan F and Yang M (2016) lsquoDesign of PLGA-based depot delivery systems for
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Wang E A et al (1990) lsquoRecombinant human bone morphogenetic protein induces bone
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Wang H et al (2012) lsquoThe use of micro- and nanospheres as functional components for
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Wang Y et al (2015) lsquoPLGAPDLLA core-shell submicron spheres sequential release
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101006jcis20028233
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White L J et al (2013) lsquoAccelerating protein release from microparticles for regenerative
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Wozney J M (1992) lsquoThe bone morphogenetic protein family and osteogenesisrsquo Molecular
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Xia Y et al (2013) lsquoProtein encapsulation in and release from monodisperse double-wall
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Xiong S et al (2011) lsquoCellular uptake of Poly-(DL-lactide-co-glycolide) (PLGA)
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152
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Xu Y et al (2017) lsquoPolymer degradation and drug delivery in PLGA-based drug-polymer
applications A review of experiments and theoriesrsquo Journal of biomedical materials
research Part B Applied biomaterials 105(6) pp 1692ndash1716 doi 101002jbmb33648
Yallapu M M et al (2010) lsquoFabrication of curcumin encapsulated PLGA nanoparticles for
improved therapeutic effects in metastatic cancer cellsrsquo Journal of colloid and interface
science 351(1) pp 19ndash29 doi 101016jjcis201005022
Yameen B et al (2014) lsquoInsight into nanoparticle cellular uptake and intracellular
targetingrsquo Journal of controlled release official journal of the Controlled Release Society 190
pp 485ndash499 doi 101016jjconrel201406038
Yang Y Y Chia H H and Chung T S (2000) lsquoEffect of preparation temperature on the
characteristics and release profiles of PLGA microspheres containing protein fabricated by
double-emulsion solvent extractionevaporation methodrsquo Journal of controlled release
official journal of the Controlled Release Society 69(1) pp 81ndash96 doi 101016s0168-
3659(00)00291-1
Yang Y Y Chung T S and Ng N P (2001) lsquoMorphology drug distribution and in vitro
release profiles of biodegradable polymeric microspheres containing protein fabricated by
double-emulsion solvent extractionevaporation methodrsquo Biomaterials 22(3) pp 231ndash
241 doi 101016s0142-9612(00)00178-2
Yilgor P et al (2009) lsquoIncorporation of a sequential BMP-2BMP-7 delivery system into
chitosan-based scaffolds for bone tissue engineeringrsquo Biomaterials 30(21) pp 3551ndash3559
doi 101016jbiomaterials200903024
Yilgor P Hasirci N and Hasirci V (2010) lsquoSequential BMP-2BMP-7 delivery from
polyester nanocapsulesrsquo Journal of biomedical materials research Part A 93(2) pp 528ndash
536 doi 101002jbma32520
153
Zhang H-X et al (2016) lsquoIn vitro and in vivo evaluation of calcium phosphate composite
scaffolds containing BMP-VEGF loaded PLGA microspheres for the treatment of avascular
necrosis of the femoral headrsquo Materials science amp engineering C Materials for biological
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Zhang S and Uludag H (2009) lsquoNanoparticulate systems for growth factor deliveryrsquo
Pharmaceutical research 26(7) pp 1561ndash1580 doi 101007s11095-009-9897-z
154
10ANEXO MATERIAL SUPLEMENTARIO
httprsbwebnihgovij
155
156
11ANEXO DE PUBLICACIONES
Review ArticleBone Regeneration from PLGA Micro-Nanoparticles
Inmaculada Ortega-Oller1 Miguel Padial-Molina1 Pablo Galindo-Moreno1
Francisco OrsquoValle2 Ana Beleacuten Joacutedar-Reyes3 and Jose Manuel Peula-Garciacutea34
1Department of Oral Surgery and Implant Dentistry University of Granada 18011 Granada Spain2Department of Pathology School of Medicine and IBIMER University of Granada 18012 Granada Spain3Biocolloid and Fluid Physics Group Department of Applied Physics University of Granada 18071 Granada Spain4Department of Applied Physics II University of Malaga 29071 Malaga Spain
Correspondence should be addressed to Jose Manuel Peula-Garcıa jmpeulaumaes
Received 27 March 2015 Accepted 4 June 2015
Academic Editor Hojae Bae
Copyright copy 2015 Inmaculada Ortega-Oller et al This is an open access article distributed under the Creative CommonsAttribution License which permits unrestricted use distribution and reproduction in any medium provided the original work isproperly cited
Poly-lactic-co-glycolic acid (PLGA) is one of the most widely used synthetic polymers for development of delivery systems fordrugs and therapeutic biomolecules and as component of tissue engineering applications Its properties and versatility allow it tobe a reference polymer in manufacturing of nano- and microparticles to encapsulate and deliver a wide variety of hydrophobic andhydrophilic molecules It additionally facilitates and extends its use to encapsulate biomolecules such as proteins or nucleic acidsthat can be released in a controlled wayThis review focuses on the use of nanomicroparticles of PLGA as a delivery system of oneof the most commonly used growth factors in bone tissue engineering the bone morphogenetic protein 2 (BMP2) Thus all theneeded requirements to reach a controlled delivery of BMP2 using PLGA particles as a main component have been examinedTheproblems and solutions for the adequate development of this system with a great potential in cell differentiation and proliferationprocesses under a bone regenerative point of view are discussed
1 Introduction
Bone regeneration is one of the main challenges facing us inthe daily clinic Immediately after a tooth extraction normalbiological processes remodel the alveolar bone limiting insome cases the possibility of future implant placementDifferent strategies for the preservation of that bone havebeen explored in recent years Other conditions such astrauma tumor resective surgery or congenital deformitiesrequire even higher technical and biological requirementsto generate the necessary bony structure for the occlusalrehabilitation of the patient To overcome these anatomicallimitations in terms of bone volume different approacheshave been proposed to either improve the implant osteoin-tegration or to augment the bone anatomy where it will beplaced [1 2] Autogenous bone graft is still considered theldquogold standardrdquo due to its osteogenic osteoconductive andosteoconductive properties [3 4] However it also presentsseveral limitations including the need for a second surgery
limited availability and morbidity in the donor area [5]Therefore other biomaterials such as allogeneic grafts withosteoconductivity and osteoinductive capacities [6 7] andxenogeneic grafts [8 9] and alloplastic biomaterials [10]with osseoconductive potential were proposed All thesematerials although acceptable are not suitable in manyconditions and usually require additional consideration inthe decision process [11] Additionally the bone quantity andquality that can be obtained with these materials are oftenlimited
The use of bioactive molecules alone or in combina-tion with the previously described materials has thereforebecome amajor area of interest thanks to their high potentialWhen using this kind of procedures it is important toconsider (1) the delivery method and (2) the molecule itselfBioactive molecules can be transported into the defect areaas a solution or a gel embedded in sponges adhered to solidscaffolds and more recently included in particles of differentsizes Using these methods PDGF (platelet-derived growth
Hindawi Publishing CorporationBioMed Research InternationalVolume 2015 Article ID 415289 18 pageshttpdxdoiorg1011552015415289
2 BioMed Research International
factor) FGF (fibroblast growth factor) IGF (insulin growthfactor) Runx2 Osterix (Osx) LIM domain mineralizationprotein (LMP) BMP (bone morphogenic protein) and morerecently periostin have been proposed as potential candidatesfor regeneration procedures within the oral cavity includingbone and periodontal tissues [12 13] These molecules havebeen tested alone or in combination with stem cells [14] usingseveral in vitro and in vivo strategies [15]
Consequently within the context of this review we intendto review the delivery methods of bioactive molecules withthe purpose of bone regeneration with a particular focus onpolymeric nanomicroparticles especially those with PLGAas main component to encapsulate the growth factor BMP-2 An overview of the biological functions of bone morpho-genetic proteins and an analysis of the different parametersaffecting the physicochemical properties of these systems arepresented Synthesis method particle size and morphologyuse of stabilizers and their incidence in the colloidal sta-bility protective function and surface functionality will bediscussed In addition we explore the different strategies thatcan be used to optimize the encapsulation efficiency andrelease kinetics main parameters that determine the correctdevelopment of polymeric carriers used in tissue-engineeredbone processes
2 BMPs Action and Regulation
For bone regeneration in particular bone morphogeneticgrowth factors (BMP) are probably the more tested groupof molecules Since 1965 when Urist [16] showed that theextracted bone BMPs could induce bone and cartilage forma-tion when implanted in animal tissue an increasing numberof reports have tested its in vivo application and biologicalfoundation when used in bone defects [17ndash19] BMPs aremembers of the TGF-120573 superfamily of proteins [20] TheBMP family of proteins groups more than 20 homodimericor heterodimericmorphogenetic proteins which functions inmany cell types and tissues not all of them being osteogenic[21] BMPs can be divided into 4 subfamilies based on theirfunction and sequence being BMP-2 BMP-4 and BMP-7 the ones with osteogenic potential [21] The actions ofBMPs include chondrogenesis osteogenesis angiogenesisand extracellular matrix synthesis [22] Within this fam-ily of proteins BMP-2 has been the most studied It hasosteoinductive properties that promote the formation of newbone by initiating stimulating and amplifying the cascadeof bone formation through chemotaxis and stimulationof proliferation and differentiation of the osteoblastic celllineage [5 17 19 20]The absence of it as studied in knockoutmodels leads to spontaneous fractures that do not heal withtime [23] In fact other models have demonstrated that theabsence of either BMP-4 [24] or BMP-7 [25] do not lead tobone formation and function impairmentwhich demonstratethe compensatory effect produced by BMP-2 alone [26]
Many cell types in bone tissue produce BMPs includingosteoprogenitor cells osteoblasts chrondrocytes plateletsand endothelial cells This secreted BMP is then stored in theextracellular matrix where it mostly interacts with collagentype IV [27] During the repair and remodeling processes
BMPs
BMPR-I BMPR-II
Smad 1 Smad 5
Smad 8Smad 4
Runx2 Dlx5 Osterix
Osteogenesis
Bone resorption
Figure 1 Schematic representation of the main BMP molecularpathway to osteogenesis BMPs interact with cell surface receptorsI and II to activate Smads 1 5 and 8 These activated Smads activateSmad 4 All together as a protein complex activate Runx2 Dlx5 andOsterix
osteoclast resorptive activity induces the release of BMPs tothe medium so that they are suspended and can interact withnearby cells to initiate the subsequent osteogenic process [28]
A BMP in the extracellular matrix binds to cell surfacereceptors BMPR-I and BMPR-II and activates the Smadcytoplasmic proteins or the MAPK pathway [29] WhenBMPR-I is activated BMPR-II is recruited and activatedas well [30] The activation of the complexes BMPR-I andBMPR-II leads to the activation of several Smads (1 5 and 8)that also activate Smad 4 and they all form protein complexesthat are transported into the nucleus where Runx2 Dlx5and Osterix genes (important in osteogenesis) are activated[26 27] (Figure 1) Similarly when the MAPK pathwayis activated it leads to induction of Runx2 transcriptionand therefore to bone differentiation [31] A number ofextracellular and intracellular antagonists have also beendescribed including noggin chordin and gremlin or Smads6 7 and 8b respectively [32]
21 Clinical Use of BMP-2 Today the BMP-2 is commerciallyavailable under different brand names and concentrations Itusually consists of a collagen absorbable sponge embeddedwith recombinant human BMP-2 In 2002 it was approvedby the FDA as an alternative of autogenous bone graftingin anterior lumbar interbody fusion [33] Later in 2007 theFDA approved the use of rhBMP-2 as an alternative forautogenous bone grafting in the increase of the alveolar crestdefects associated with the tooth extraction maxillary sinuspneumatization [33]
Beside the applications in spine clinical studies wherevery high concentrations are used (AMPLIFY rhBMP-240mg) clinical studies have supported its use in the oral
BioMed Research International 3
cavity BMPs have been used in periodontal regenerationbone healing implant osteointegration oral surgery withorthodontic purposes bone pathology sequel repair distrac-tion osteogenesis and endodontic reparative surgery [28 34]However it has shownmore promising results in cases whereonly bone tissue is to be regenerated including preimplantsite development sinus lift vertical and horizontal ridgeaugmentation and dental implant wound healing [35] In thissense it has been shown that the use of rhBMP-2 induced theformation of bone suitable for placement of dental implantsand their osteointegration [36] Furthermore it appears thatthe newly formed bone has similar properties to the nativebone and is therefore capable of supporting denture occlusalforces [37] In the particular case of sinus lifting where bonedeficiency is greater and therefore supportive therapies canbe more helpful a recent meta-analysis found a total of 3human studies and 4 animal trials (Table 1) [38] In summarythe included studies concluded that rhBMP-2 induces newbone formation with comparable bone quality and quantityof newly formed bone to that induced by autogenous bonegraft In some cases even higher bone quality and quantityhave been reported [39]
Conversely recent studies report severe complicationsafter its use [61] Even more high doses have also associatedwith carcinogenic effects which led the authors to emphasizethe need for better guidelines in BMP clinical use [62] Notso drastic recent studies are highlighting the negative sideeffects and risks of its application making high emphasison potential bias of nonreproducible industry sponsoredresearch especially when used in spinal fusion [44 63 64]The use of rhBMP-2 has been shown to increase the risks forwound complications and dysphagia with high effectivenessand harms misrepresentation through selective reportingduplicate publication and underreporting [44] Specificallyin oral bone regenerative applications a report in sinus liftconcluded that the use of BMP-2 promotes negative effectson bone formation when combined with anorganic bovinebone matrix versus anorganic bovine bone alone [41] incontrast with previous reports and reviews [38] Takingtogether this information it can be concluded that it is ofextreme importance to be careful with the clinical use of newproducts avoiding off-label applications It is also importantto highlight the need for more and better clinical research
To overcome these limitations new strategies such asthe use of ex vivo BMP-2-engineered autologous MSCs [65]encapsulation of the protein in different biomaterials ordelivery by gene therapy are being explored in recent years
The development of these technologies is based on somebiological facts In vitro effects of BMPs are observed at verylow dosages (5ndash20 ngmL) although current commerciallyavailable rhBMPs are used in large dosages (up to 40mg ofsome products) [28] This is probably due to an intense pro-teolytic consumption during the early postsurgical phases Itis important to know the proper sequence of biological eventsthat lead to normal tissue healing Then this knowledge canbe used to intervene at the specific time frame where ourtherapy is intended to act [15] Effective bone formation asdescribed above is a sequential processTherefore the induc-tive agent should be delivered at a maintained concentration
during a timeframe In this sense as in many other processesin medicine it has been recently demonstrated that long-term release of BMP-2 is more effective than short-term overa range of doses [51] It is also important to note that therole of other molecular pathways and crosstalk between thedifferent components playing in bone regeneration is notperfectly understood yet and therefore more research hasto be conducted
What is known so far in summary is that BMPs specif-ically BMP-2 is of utility for promoting bone regeneration[28] However the currently FDA-approved BMP-2 deliverysystem (INFUSE Medtronic Sofamor Danek Inc) presentsimportant limitations [66] Firstly protein is quickly inacti-vatedTherefore its biological action disappears maybe evenbefore the blood clot that forms after the surgery is beingorganized Second the recombinant protein is delivered inan absorbable collagen sponge Thus the distribution ofthe BMP in a liquid suspension embedded into a collagensponge makes it impossible to be certain that the protein isreaching the ideal target Therefore where when and forhow long a dose of BMP-2 is reached (determined by thedelivery method) are important factors Because of that newforms of BMP-2 delivery are being developed These newtechnologies have to guarantee a higher half-life of the proteinand a stepped release to increase the effects on the desiredcell targets The biotechnology opens the door to be able toprovide a solution to these limitations
Biodegradable nanoparticles (nanospheres and nanocap-sules) have developed as a promising important tool forthe delivery of macromolecules via parenteral mucous andtopical applications [67ndash70] Well-established biodegradablepolymers such as poly(acid D L-lactic) or poly(D L-lactic-co-glycolic) have been widely used in the preparation ofnanoparticles in recent decades because of its biocompati-bility and full biodegradability [71] However it is knownthat certain macromolecules such as proteins or peptidesmay lose activity during their encapsulation storage deliveryand release [72] To overcome this problem the addition ofstabilizers such as oxide polyethylene (PEO) or the coencap-sulation with other macromolecules and its derivatives seemto be a promising strategy
3 Polymeric Colloidal Particles to EncapsulateHydrophilic Molecules
Generally polymeric colloidal particles are hard systemswith a homogeneous spherical shape composed by naturalor synthetic polymers In order to encapsulate hydrophilicmolecules as proteins or nucleic acids it is necessary to opti-mize the polymeric composition and the synthesis methodIn this process a high encapsulation efficiency maintenanceof the biological activity of the encapsulated biomolecule andobtaining of an adequate release pattern have to be achieved[73ndash75] Several delivery systems of BMP2 (and other growthfactors GFs) using polymeric particles have been describedin the literature Most of them are microparticulated systemsusing the biocompatible and biodegradable PLGA copoly-mer as main component [76 77] Taking into account theincorporation of BMP2 to the carrier system encapsulation
4 BioMed Research InternationalTa
ble1
Sum
maryo
fclin
icalandanim
alstu
diesusingB
MP-2for
sinus
floor
elevatio
n(adapted
from[38])Th
eincludedstu
diesoverallcon
cludedthatrhBM
P-2ind
ucesnewbo
neform
ation
with
comparableb
oneq
ualityandqu
antityof
newlyform
edbo
neto
thatindu
cedby
autogeno
usbo
negraft
Reference
Stud
ydesig
nFo
llow-up
(mon
ths)
Species
(sub
jects)
Coreb
iopsy
harvestin
g(m
onths)
Graftmaterial
New
lyform
edbo
neBo
neheight
gain
(mm)
Bone
width
gain
(mm)
Bone
density
(mgmL)
Immun
erespon
seHistolog
y
Boyn
eetal
2005
[37]
RCT
52Hum
an(48)
6ndash11
075
mgmL
rhBM
P-2AC
S
NA
1129
Crest202
Midpo
int854
Apical118
684
Non
eNA
150m
gmL
rhBM
P-2AC
S047
Crest19
8Midpo
int78
0Ap
ical1078
134
Autogeno
usbo
negraft
autogenou
sbo
negraft
+allogeneicbo
negraft
1016
Crest466
Midpo
int
1017
Apical1056
350
Triplettetal
2009
[40]
RCT
58Hum
an(160)
6
150m
gmL
rhBM
P-2AC
S
NA
783plusmn352
NA
200
Non
e
Rich
vascular
marrowspaceh
ighin
cellu
larc
ontent
Autogeno
usbo
negraft
(iliacc
rest
tibia
ororalcavity)
autogeno
usbo
negraft
+allogeneic
bone
graft
946plusmn411
283
Oste
oclasts
stillpresenthigh
erfib
rous
tissue
Kaoetal2012
[41]
Prospective
6ndash9
Hum
an(22)
6ndash9
rhBM
P-2AC
S+
ABB
1604plusmn74
5
NA
NA
NA
Non
e
Fewer
ABB
particleslessnewlyform
edbo
ne(w
oven
andmatureb
ones
tructure)
ABB
2485plusmn582
MoreA
BBparticlesrem
aining
higher
newlyform
edbo
ne(w
oven
andmatured
bone
structure)
Nevinse
tal
1996
[36]
Prospective
12Goat(6)
12
rhBM
P-2AC
S
NA
NA
NA
NA
Non
e
Dense
isolatedtrabeculae
andbo
nemarrowoste
oblastandosteoclasts
no
corticalbo
ne
ACSBu
ffer
Collageno
usconn
ectiv
etissuen
oevidence
ofinflammation
noneo-osteogenesis
Hanisc
hetal
1997
[42]
RCT
24Non
human
prim
ate(12)
24rhBM
P-2AC
SNA
60plusmn03
NA
144plusmn29
NA
New
lyform
edbo
neindisting
uishable
from
resid
ualbon
eAC
S26plusmn03
139plusmn46
Wadae
tal
2001
[43]
Prospective
8Ra
bbit(10)
8rhBM
P-2AC
S224plusmn44
NA
NA
NA
NA
Cortic
albo
neform
ationin
both
grou
ps
trabeculae
with
clear
lamellarstructure
weree
mbedd
edin
fatty
marrow
Autogeno
usbo
negraft
(iliacc
rest)
219plusmn45
Leee
tal2013
[39]
Prospective
8Mini-p
ig(8)
8rhBM
P-2AC
SNA
93plusmn05
NA
519plusmn3
NA
New
lyform
edcancellous
bonenew
bone
continuo
uswith
resid
entb
onewoven
bone
infib
rovascular
andfatty
marrow
Autogeno
usbo
negraft
(iliacc
rest)
86plusmn07
329plusmn25
Irregu
lara
ndvaria
bleb
onea
mon
gdifferent
subjects
NAnot
availableRC
Trand
omized
clinicaltrialAC
Sabsorbablecollagenspon
geA
BBano
rganicbo
vine
bone
BioMed Research International 5
Hydrophilic biomolecules stabilizers
Organicphase
PLGA and stabilizers(surfactant other polymers) in
DCM acetone or EtAc
Mixture underagitationsonication
Ethanol water surfactants
Antibody
Core
PLGA BSABMP-2
Surface
Surfactant
MicronanosphereMicronanocapsule
ImmunoparticleDirected delivery
Organic solventextraction under
vacuumAqueous phase w1
First w1oemulsion
emulsion
Second polarphase w2
Final w1ow2
120
80
40
150mL
120
80
40
150mL
Figure 2 Double emulsion procedure (wateroilwater emulsion W1OW
2) to obtain PLGA micronanoparticles Depending on the
synthesis conditions (stabilizers solvents and mixing procedure) it is possible to obtain micro-nanospheres with a uniformmatrix or micro-nanocapsules with a core-shell structure Immunoparticles used for directed delivery can be obtained by attaching specific antibodymoleculeson the particle surface
is preferred to absorption because the growth factors aremore protected against environmental factors in the mediumand may have better control over the delivery and release toachieve the desired concentrations in specific site and time[78]
Normally if the GFs are related with bone regenerationprocesses nano-microparticles are trapped in a second sys-tem as hydrogels or tissue engineering scaffolds which alsoplay an important role in the release profile of GFs fromthese particles [78] The nano-microparticles have allowedthe development of multiscale scaffold thereby facilitatingcontrol of the internal architecture and adequate patterns ofmechanical gradients of cells and signaling factors [79]
All steps from the synthesis method and its characteris-tics the encapsulation process or the final surface modifica-tion for a targeted delivery determine the characteristics ofthese systems and their main goal the controlled release ofbioactive GFs
31 Synthesis Methods It is possible to found several pro-cedures to encapsulate hydrophilic molecules as proteinsor nucleic acids in polymeric nanomicroparticles Phase
separation [80] or spray drying [81] techniques have beenreported to encapsulate hydrophilic molecules However inthe case of proteins the most normally used procedureto encapsulate them into PLGA micro- and nanoparticlesis the double-emulsion (wateroilwater WOW) solventevaporation technique [75 82] A schematic description ofthis technique is presented in Figure 2 In a general wayPLGA is dissolved in an organic solvent and emulsified usingmechanical agitation or sonication with water containingan appropriate amount of protein Thus a primary wateroil(WO) emulsion is obtained In the second phase thisemulsion is poured into a large polar phase leading to animmediate precipitation of the particles as a consequenceof the polymer shrinkage around droplets of the primaryemulsion This phase may be composed of a water solutionof a stabilizer (surfactant) or ethanol-water mixtures [8384] After stirring the organic solvent is rapidly extractedby evaporation under vacuum A wide list of differentmodifications have been tested in this procedure in order toobtain a micronanocarrier system with adequate colloidalstability high encapsulation efficiency adequate bioactivityand finally a long-time release profilewith low ldquoinitial burstrdquo
6 BioMed Research International
The goal is to avoid a high amount of protein (gt60) beingreleased very quickly (24 hours) which is one of the biggestproblems of a controlled release system [76]
32 Organic Solvent Hans and Lowman show differentexamples of organic solvents used in multiple emulsionprocesses Normally dichloromethane (DMC) ethyl acetateacetone and their mixtures can be used [82] In the first stepa good organic solvent with low water solubility to facilitatethe emulsification process and low boiling point for an easyevaporation would be the election However the structureof the encapsulated protein molecules can be affected anddenaturation processes and loss of biological activity appearwhen they interact with a typical organic solvent as DMC[73] Ethyl acetate on the other hand exerts less denaturatingeffects with a lower incidence on the bioactivity of theencapsulated proteins [85]
Other important factors related with the organic solventare their physical properties that affect how the polymertails self-organize in the shell of the emulsion droplets andmodify the nanoparticle morphology and the encapsulationefficiency [86] In this way a higher water solubility ofthe organic solvent that is ethyl acetate favors a rapidsolvent removal Additionally the solvent removal rate canbe controlled by adjusting the volume of the polar phase aswell as the shear stress during the second emulsification stepAn increase of these two parameters increases the diffusionrate of ethyl acetate from primary microparticles to outeraqueous phase resulting in their rapid solidification [87] Italso enhances the encapsulation efficiency andminimizes thecontact-time between protein molecules and organic solvent[88] obtaining at the same time a lower burst effect and aslower drug release from the microparticles [87]
33 Particle Size and Morphology Particle size is an impor-tant parameter and one of the main goals of the deliverypolymeric system Microspheres from a few micrometers upto 100 120583m are suitable for oral delivery mucosal adhesion orinside scaffold use that is for bone regeneration Nanoscaledimension of the carrier offers enhanced versatility whencompared with particles of larger size This is due to thefact that they have higher colloidal stability improved dis-persibility and bioavailability more reactive surface and alsocan deliver proteins or drugs inside and outside of thecorresponding cells [89] BMP2 promotes bone formationand induces the expression of other BMPs and initiates thesignaling pathway from the cell surface by binding to twodifferent surface receptors [22] Therefore the BMP2 carrierparticles must release it into the extracellular medium Sincecellular intake of PLGAnanoparticles is very fast the intakingprocess can be limited by an increase in size from nano-to microparticles [90] However the interaction betweenparticles and cells is strongly influenced by particle size Ifcell internalization is desired the particle must be comprisedin the submicron scale at an interval between 2 and 500 nm[91] Moreover this size is needed for a rapid distributionafter parenteral administration in order to reach differenttissues through different biological barriers In addition
Figure 3 Scanning electron microscopy (SEM) photography ofPLGA nanoparticles obtained by a double emulsion emulsificationprocedureThis systemwith spherical shape low polydispersity andnanoscopic scale shows the intended properties for an adequatephysiological distribution and cell internalization
the intake by macrophages is minimized with a diameterof nanoparticles under 200 nm and even smaller [82 92]As discussed by Yang et al [93] slight modifications ofthe synthesis procedure can suppose drastic effects on thesize or particle morphology and therefore in the proteinencapsulation efficiency and kinetic release
In double emulsion processes the first emulsificationstep largely determines the particle size while the secondemulsification step characterized by the solvent eliminationand polymer precipitation mainly affects the particle mor-phology [86] However the use of surfactant solutions asthe polar medium of the second emulsification process andthe volume ratio between organic and polar phases in thisstep has shown an important influence in the final size [94]Therefore the correct election of the organic solvent thepolymer concentration the addition of surfactant and theemulsification energy allow controlling the size of the system
The incorporation of poloxamers (F68) in the organicsolvent of the primary emulsification helps to increase thecolloidal stability of the first dispersion by being placedat the wateroil interface This reduces the particle size incomparison with pure PLGA nanoparticles in which theonly stability source comes from electric charge of thecarboxyl groups of the PLGA [95] It is normal to obtainspherical micronanospheres with a polymeric porous coreA typical SEM micrograph of PLGA nanoparticles obtainedby WOW emulsion using a mixture of organic solvents(DCMacetone) and ethanolwater as second polar mediumis shown in Figure 3 in which the spherical shape anduniform size distribution are the main characteristics Theouter polymeric shell in the second emulsification steppushed the water droplets to the inner core according to theirsolidification process [96] This process allows producingparticles like capsules with a core-shell structure in whichthe inner core has a low polymer density Figure 4 shows atypical core-shell structure in which the polymer precipitatesand shrinks around the water droplets during the solventchange of the second phase and the subsequent organicsolvent evaporation process [97] In this case the process of
BioMed Research International 7
(a) (b)
Figure 4 PLGApoloxamers188 blend nanoparticles (a) Scanning transmission electron microscopy (STEM) photography (b) scanningelectron microscopy (SEM) photography STEM technique allows the analysis of the nanoparticle structure with an internal region with alow polymer density which is representative of nanocapsules with core-shell structure
solidification of the polymer is influenced and determined bythe miscibility of the organic solvent with the second polarphase and the removal rate
The polymeric shell often presents channels or pores asa consequence of the inner water extrusion due to osmoticforcesThis can reduce the encapsulation efficiency and favorsa fast initial leakage with the unwanted ldquoburst releaserdquo [93]This modification of internal structure of the particles isusually indicated assigning the term ldquonanosphererdquo to thesystem with a core consisting of a homogeneous polymermatrix The bioactive agent is dispersed within them whilethe core-shell structure would be similar to a ldquonanocapsulerdquowhere the biomolecule is preferably in the aqueous cavitysurrounded by the polymeric shell [78] (see Figure 2)
34 Stabilizer Agents
341 Colloidal Stability The double emulsion method nor-mally requires the presence of stabilizers in order to confercolloidal stability during the first emulsification step toprevent the coalescence of the emulsion droplets and latertomaintain the stability of the final nanomicroparticles [98]Polyvinyl alcohol (PVA) and PEO derivate as poloxamers(also named pluronics) have been used inmost cases [83 94]Others include natural surfactants such as phospholipids[99 100] In some cases it is possible to avoid surfactantsif the particles have an electrostatic stability contributionthat is from the uncapped end carboxyl groups of the PLGAmolecules [101]
As it has been previously commented PVA and polox-amers have shown their efficiency in synthetizing both nano-and microparticles affecting not only the stability of thesystems but also their size and morphology Thus a sizereduction effect has been found using PVA in the externalwater phase affecting at the same time the surface porosity
mainly in microsized particles [94] A comparative studybetween this and phospholipids (di-palmitoyl phosphaty-dilcholine DPPC) as stabilizers showed that DPPC couldbe a better emulsifier than PVA to produce nano- andmicroparticles With this method a much lower amount ofstabilizer was needed to obtain a similar size In the samestudy a higher porosity on the particle surface for the PVAemulsified nanospheres was shown [99]
On the other hand the combination of PLGA withpoloxamers has shown positive effects for the nano- andmicrosystems in terms of stability [102] The use of thesesurfactants in the first or second steps of the WOWemulsion procedure leads to different situations Thus ifpoloxamers are blended with PLGA in the organic phaseof the primary emulsification an alteration of the surfaceroughness is obtained However if these are added in theinner water phase an increase of porosity is found [83] Inaddition their inclusion in the polar phase of the secondemulsification step also generates hydrophilic roughnesssurfaces A quantification of this is shown in Figure 5 inwhich the electrophoretic mobility of both PLGA pure andPLGApluronic F68 nanoparticles is measured as a functionof the pH of the medium The observed dependence withthis parameter is a consequence of the weak acid characterof the PLGA carboxyl groups When poloxamer moleculesare present at the interface a systematic reduction ofmobilitywas found as a consequence of the increase in the surfaceroughness The hydrophilic surfactant chains spread outtowards the solvent originating a displacement of the shearplane and the consequent mobility reduction [95 101]
The final PLGA particle size is primarily controlled byelectrostatic forces and is not significantly affected by thepresence or nature of poloxamer stabilizers [101] The recog-nition of the nanocarriers by the mononuclear phagocyticsystem (MPS) can be significantly altered if the surface of
8 BioMed Research International
pH4 5 6 7 8 9
minus6
minus4
minus2
0
2
120583e
(V m
minus1
sminus1)
Figure 5 Electrophoretic mobility versus pH for PLGA nano-particles with different characteristics (998787) PLGA (◼) PLGApolox-amer188 blend and (∙) PLGA covered by Immuno-120574-globulin Thedifferent surface composition affects the electrokinetic behaviourof bare nanoparticles Surface charge values were screened by thepresence of nonionic surfactant as poloxamers or in a higherextension by the presence of antibody molecules attached on thesurface
colloidal particles is modified by using PEO block copoly-mer of the poloxamer molecules The steric barrier givenby these surfactant molecules prevents or minimizes theadsorption of plasma protein and decreases the recognitionby macrophages [103] The size of microspheres is alsounaffected by the coencapsulation of poloxamers The sys-tem containing poloxamer-PLGA blends drive to an innerstructure displaying small holes and cavities in relation withmicrospheres of pure PLGA with a compact matrix-typestructure [83]
Microparticles formulated by poloxamer in the secondpolar medium have completely different surface than thePVA ones almost without pores [94] A comparison betweendifferent poloxamers shows that the hydrophilic-lipophylicbalance (HBL) of the surfactant plays a crucial role determin-ing the surfactant-polymer interactions and controlling theporosity and roughness of the nano-microparticles [83 104]
In a similar manner to surfactants polymer character-istics like the hydrophobicity grade the molecular weightor the hydrolysis degradation rate can strongly influencethe particlemorphologyTherefore the polymer compositionof the particles greatly affects its structure and propertiesThis is why it is usual to use other polymers in order tomodify the behavior and application of the particles Inthis way polyethylene glycol (PEG) of different chain lengthis frequently used to modify the surface characteristicsWith PEG particles are more hydrophilic and with roughersurfaces which affects the MPS action by increasing thecirculating-time and half-life in vivo like the presence of PEOchains [105] Additionally PEG chains also provide colloidalstability via steric stabilization Pegylated-PLGA nano- ormicroparticles can be normally obtained by using in the
synthesis method PLGAPEG di- and triblock copolymers[58 59 75] Natural polymers as chitosan besides modifyingthe hydrophobicity-hydrophilicity ratio of the surface alsoconfer them a mucoadhesive character [106]
342 Encapsulation Efficiency and Bioactivity Furthermorethe use of stabilizers (surfactants or polymers) also influ-ences the encapsulation efficiency and the protein stabilityIn fact for the WOW solvent evaporation process thechlorinated organic solvent used for the first emulsificationcould degrade protein molecules encapsulated in this stepif they come into contact with the organicwater interfacecausing their aggregation or denaturation [107]Thepolymer-protein interaction the shear stress for the emulsificationprocess and the pH reduction derived from PLGA polymerdegradation can also produce the same situation with thesubsequent loss of biological activity of the encapsulatedbiomolecules Different strategies to prevent it have beenused For example an increase of the viscosity around proteinmolecules can help to isolate them from their microenviron-ment [108] In this way viscous products such as starch havebeen used to prevent protein instability [109] These authorscoencapsulate BMP2 with albumin inside starch microparti-cles using other biodegradable polymer poly-120576-caprolactoneinstead of PLGA The BMP2 retained its bioactivity Despitea low encapsulation rate beside an initial burst followedby an uncompleted release the amount of BMP2 neededat the beginning was lower [109] The combination of PEOsurfactants with PLGA (blended in the organic phase) canalso preserve the bioactivity of microencapsulated proteins[110] or nucleic acids [84]
However in most cases the coencapsulation of GFswith other biomolecules was the preferred strategy Therebyserum albumins (SA) have shown the capacity to limit theaggregation-destabilization of several proteins incited by thewaterorganic solvent interface of the primary emulsificationprocess [111 112] White et al encapsulated lysozyme insidePLGA-PEG microparticles In addition to the protectivefunction they also observed an important increase of theentrapment efficiency when human SA was coencapsulatedwith lysozyme and BMP2 [59] drsquoAngelo et al used heparinas stabilizer because it forms a specific complex with severalGFs stabilizes their tridimensional structure and promotestheir bioactivity An encapsulation efficiency of 35 wasincreased to 87 using bovine SA as a second stabilizer toencapsulate two natural proangiogenic growth factors insidePLGA-poloxamer blended nanoparticlesThe in vitro cellularassays showed the preservation of the biological activity ofGFs up to one month [56]
The use of more hydrophilic surfactants (poloxamers)or polymers (PEG) in the inner water phase or blendedwith PLGA in the organic phase of the primary emulsionreduces the interaction of encapsulated proteins with thehydrophobic PLGA matrix This prevents disrupting thestructure of the protein molecules and helps at the sametime to neutralize the acidity generated by the hydrolyticdegradation of the PLGA [113] In some cases the combina-tion of several stabilizers such as poloxamers trehalose andsodium bicarbonate has been shown to preserve the integrity
BioMed Research International 9
of encapsulated proteins but it also reduces the encapsulationefficiency [114]
As a general rule encapsulation efficiency increases withthe size of the particles [82] Additionally the adequate sta-bilization of the primary emulsion by amphiphilic polymersand a rapid solidification (precipitation) of polymer in thesecond step are favorable parameters for enhancing proteinentrapment efficiency in the WOW emulsion technique[87]
The tendency of BMP2 to interact with hydrophobicsurfacesmay decrease the loss of encapsulated protein duringthe extraction of the solvent phase This favors a higherentrapment but it lowers the later extraction [58] An optimalprotein encapsulation is obtained when pH of the internaland external water phases is near the isoelectric point of theprotein [92] Blanco and Alonso [83] observed a reductionin the protein encapsulation efficiency when poloxamer wascoencapsulated in the primary emulsion This highlights themain role played by the protein-polymer interaction in theencapsulation efficiency and the later release process How-ever too much emulsifier may also result in a reduction ofthe encapsulation efficiency [99] Therefore an equilibriumbetween the emulsification powder of the surfactant and theirconcentration is needed
35 Release Profile The release profile represents one of themost important characteristics of a nanomicro particulatecarrier system since their development has a main finalobjective the adequate release of the encapsulated bioactivemolecules to reach the desired clinical action
The release pattern of protein encapsulated in PLGAmicronanoparticles can present different behavior It ispossible to find a continuous release when the diffusionof the biomolecule is faster than the particle erosion Thisprocess involves a continuous diffusion of the protein fromthe polymer matrix before the PLGA particle is degradedin lactic and glycolic acid monomers by hydrolysis [74] Abiphasic release characterized by an initial burst at or nearthe particle surface followed by a second phase in whichprotein is progressively released by diffusion has also beendescribedThe second phase can be enhanced by bulk erosionof PLGA shell and matrix which results in an importantincrease of pores and channels [75] A third triphasic releaseprofile has been found when a lag release period occursafter initial burst and until polymer degradation starts [115]Finally it is possible to obtain an incomplete protein releaseas a consequence of additional factors related with theprotein-polymer interaction or protein instability Figure 6illustrates the different release profiles previously describedThe optimal carrier system should be capable of releasinga controlled concentration gradient of growth factors inthe appropriate time preventing or at least reducing orcontrolling the initial burst effect [116] A controlled initialburst followed by a sustained release significantly improvesthe in vivo bone regeneration [117ndash119]
Giteau et al [108] present an interesting revision on ldquoHowto achieve a sustained and complete release from PLGAmicroparticlesrdquo They begin by analyzing the influence of therelease medium and sampling method on the release profile
00
20
40
60
80
100
Cum
ulat
ive r
eleas
e of p
rote
in (
)
Time (h)500400300200100
Figure 6 Release profiles (I) BSA release from PLGA nanoparti-cles with high initial burst release (red dots line) biphasic modelcombining a moderate initial burst and a subsequent sustainedrelease (blue dash line) triphasicmodel with a lag of release betweenboth initial and sustained release phases (dash-dot green line)incomplete release
and highlight the significance of the centrifugation cleaningprocess or the releasemedium volume Adjusting to adequatevalues the centrifugation speed or the buffer volume itis possible to separate micronanoparticles from protein-containing release medium in a very easy wayThis allows forstable and reproducible release patterns On the other handto ensure a better protein release profile modification of themicroparticle formulation and microencapsulation processin order to preserve protein aggregation has to be performedProtein stability has to be maintained by preventing theformation of harmful medium For example the synthesisformulation can be modified to use more hydrophilic poly-mers since they have been shown to reduce the initial burstand to deliver bioactive proteins over long time periods
The most relevant strategies are referenced below Drugrelease from PLGA nanomicroparticles can be controlledby the polymer molecular weight and the relation betweenmonomers (lactideglycolide) so that an increase in gly-colic acid accelerates the weight loss of polymer due tothe higher hydrophilicity of the matrix [75] A mixtureof different PLGA nanoparticles obtained using 50 50 and75 50 latideglycolide ratio has shown a great potential forprotein drug delivery with a higher initial burst from PLGA50 50 A slow release period has been observed for PLGA75 50 encapsulating a glycoprotein (120572-1-antitrypsin) withclinic activity in some pulmonary diseases [60]
On the other hand a faster erosion of the microsphereswith reduction in the PLGA molecular weight due to thefacility of water penetration and the subsequent polymerdegradation has been described [83] Schrier et al workingwithmicrospheres prepared by wow using different types ofPLGA analyzed the important role of the molecular weightlactide-glycolide relation and acid residues [57]The amountof rhBMP2 adsorbed on the microparticle surface increased
10 BioMed Research International
with the hydrophobicity of the polymer At the same time therelease was in correlation with the degradation profile of thedifferent polymers [57]
Thus the use of more hydrophilic polymers reduces thehydrophobic protein-polymer interaction This effect favorsa more homogeneous distribution in the polymer matrixand increases the water uptake in the microspheres Thusthe release rate of rhBMP2 encapsulated in microspherescomposed by a PEG-PLGA di-block copolymer is increasedwith the PEG content of the polymer matrix [58] A similarresult was obtained using PLGA-PEG-PLGA triblock copoly-mers [59] In this case modifying the monomer relation(lactide-glycolide) in the PLGA and increasing the amountof PLGA-PEG-PLGA in the formulations the release profileof BMP-2 coencapsulated with human SA in microesphereswas adjustable Similarly the interaction of lysozyme withpoloxamer 188 before their encapsulation produces a sus-tained release over 3 weeks without any burst effect In thesame line using PLGA-PEG-PLGA as polymer a sustainedrelease of bioactive lysozime was extended over 45 days whenthe protein was complexed with poloxamer 188 previously tothe encapsulation [120] However the presence of PEG300 asan additive of the inner phase of microparticles during theencapsulation process also influences the protein distributionand the release profile In this case there is a decrease of theinitial burst but with less overall release [58]
On the other hand the use of PLGA-poloxamers blendsis useful to obtain a sustained release for more than onemonthwithout any incidence in the high initial burst [56 92]However for an encapsulated plasmid inside nanoparticlesobtained by PLGA-poloxamer blends the hydrophobicity ofthe surfactant allows prolonging the release up to 2 weeks in acontrolledmannerMoreover a complete release was reachedfor the PLGA-poloxamer blend instead PLGA nanoparticlesin which the maximum release was around 40 [84]
PLGA and poloxamers (pluronic F68) blends can also beused to obtain nanocomposite vesicles by a double emulsionprocess These vesicles are suitable for the encapsulation ofhydrophobic and hydrophilic molecules The presence ofpluronic affects the colloidal stability of the vesicles and therelease pattern of the encapsulated molecules These vesiclespresent a wall of 30 nm and the drug is encapsulated in thepresence of the poloxamer [121]
Other strategies include the use of different compounds toincrease the release timeThus BMP2 encapsulated in PLGA-PVA nanoparticles (around 300 nm) showed higher encapsu-lation efficiency and a short-time release profile with a veryhigh initial burst However with the same synthesis proce-dure (wow) but using PHBV (Poly(3 hydroxybutyrateco-3-hydroxivalerate)) BMP7 loaded nanocapsules had lessencapsulation efficiency despite a long-time delivery Nev-ertheless the maximum released amount was lower Thisdifference in the release profile was due to the differencein hydrophilicity and degradation rates of both polymers[122] Similarly PLGA-poloxamer blend nanoparticles weresuperficially modified by introducing chitosan in the secondstep of the synthesisThis method showed a sustained releaseprofile for up to 14 days without any initial important burstIn this case a recombinant hepatitis B antigen was used
[106] Moreover the use of heparin conjugated with PLGAporous microspheres has also been described to obtain along-time delivery system reducing at the same time theinitial burst In these systems heparin was immobilized ontothe nanomicroparticle surface The release was controlledby using the binding affinities of heparin to several growthfactors including BMP2 In this case the initial burst wasreduced to 4ndash7 during first day followed by a sustainedrelease of about 1 per day [51ndash53]
The initial burst release may be attenuated by thefabrication of double-wall microspheres that is core-shellmicroparticles The presence of a PLA shell reduces therelease rate of BSA encapsulated in the PLGA core andextends the duration of the release profile up to two monthsMoreover an increase in the PLA molecular weight influ-ences the rate of particle erosion which further slows theprotein release [123]
The modification of the viscosity in the environmentof microparticles additionally influences the release patternViscosity can control the burst at earliest time point andpromote a sustained release This situation has been shownfor rhBMP2-PLGA microspheres embedded in a chitosan-thioglycolic acid hydrogel (Poloxamer 407) [124] Yilgor etal also incorporated the nanoparticles of their sequentialdelivery system into a scaffold composed by chitosan andchitosan-PEO [54] In other work PLGAPVA microsphereswith encapsulated BMP2 were combined with differentcomposite biomaterials (gelatin hydrogel or polypropylenefumarate) The sustained release of the bioactive moleculewas extended over a period of 42 days In vivo results indicatethe importance of the composite characteristics In this casean enhanced bone formation was obtained when the PLGAmicroparticles were incorporated into the more hydrophobicmatrix (polypropylene fumarate) [125 126]
Finally Table 2 summarizes important information aboutdifferent parameters related to the use of PLGA basednano- ormicroparticles to encapsulate transport and releasegrowth factors (mainly BMP2)
36 Gene Therapy for Bone Tissue Engineering DirectedDelivery In the last years gene therapy has begun to playa role in bone tissue regeneration becoming an alternativemethod for the delivery of BMP2 [127 128] Thus the genesencoding a specific protein can be delivered to a specific cellrather than the proteins themselves To reach this purposean efficient gene vector is necessary Viral vectors possess thebest transfection efficiency but numerous disadvantages themost notable of them being the risk of mutagenesis Nonviralvectors elude these problems but with a significant reductionin the transfection rate [129]Therefore intracellular deliveryof bioactive agents has become the most used strategy forgene therapy looking for the adequate transfection andconsequent expression of the desired protein [79]
PLGA microspheres obtained by a wow double emul-sion process have been used by Qiao et al to entrap plasmid-BMP2polyethyleneimine nanoparticles In this case a sus-tained release of these nanoparticles until 35 days without ini-tial burst was found resulting in differentiation of osteoblast
BioMed Research International 11
Table 2 Nanomicroparticles systems to encapsulate GFs mainly BMP2 growth factor Most of them are in the microscopic scale andwere used to be entrapped into scaffold of different characteristics PVA has been the more used surfactant-stabilizer It is possible to findboth encapsulation and surface adsorption of the growth factors with high-moderate efficiency The use of heparin as stabilizer reducessignificantly the initial burst release favoring a sustained release in the time The bioactivity of the GF was preserved in most of the systemsand coencapsulation with other biomolecules seems to have a similar effect than the use of surfactants as stabilizers
Polymers Stabilizer Size Encapsulation EE Release Biological activity Reference
PLGA PVA 10ndash20120583m AdsorbedrhBMP2
20 ngmL ofconstant sustained
release
Better boneformation after 8
weeksFu et al 2013 [44]
PLGA PVA 10ndash100120583m rhBMP2-BSA69 (BMP)
Burst (20)Sustained until77 (28 days)
BMP2 moleculeswith bioactivity Tian et al 2012 [45]
PLGA 75 25 PVA 182120583m 82 mdash
Good bonedefect repair
outcomes within8minus12 weeks
Rodrıguez-Evoraet al 2014 [46]
PLGA PVA 228120583m 605
30 initial burstSlower release of4 per week After
8 weeks 60released
No loss ofbioactivity
Reyes et al 2013[47]
PLGAPEGNo doubleemulsionsynthesis
100ndash200 120583m Adsorbed BMP2
13 initial burstSlower release of001ndash8 per dayAfter 23 days 70
released
Substantial boneregeneration of the
scaffold
Rahman et al 2014[48]
Different PLGA PVA 20ndash100 120583m
30 (uncappedPLGA)
90 (cappedPLGA)
26ndash49 (1 day)Total after 2 weeks
No loss ofbioactivity
Lupu-Haber et al2013 [49]
PLGA 75 25 PVA 5ndash125 120583m mdashInitial burst 30 (1
day)Sustained 35 days
Higher volumesand surface areacoverage of new
bone
Wink et al 2014[50]
PLGA Heparin 200ndash800 nm Adsorbed BMP294
No initial burstSustained over 4
weeks
Significantreduction of theBMP2 dose forgood boneformation
La et al 2010 [51]
PLGA Heparin-Poloxamer 160 nm Adsorbed BMP2
100
Initial burst(4ndash7) linear
profile
Higher matrixmineralization ofregenerated bone
Chung et al 2007[52]
PLGA Heparin 100ndash250 nm Adsorbed 94Initial burst 10 (1
day)60 after 30 days
No loss ofbioactivityEfficacy of
administrationamount 50-fold
lower
Jeon et al 2008 [53]
PLGA PVA sim300 nm 80 85 initial burst (1day)
No loss ofbioactivity
Yilgor et al 2009[54]
PLGA (in rings) PVA 215 120583m 66Moderate burstSustained releaseover 6 weeks
60 of calvariadefect were healed
Rodrıguez-Evoraet al 2013 [55]
PLGA-Poloxamer 188Blend
Poloxamer 150 nmFGF-BSA-Heparin60ndash80
40 initial burst (1day) 60 (30 days)
No loss ofbioactivity
drsquoAngelo et al 2010[56]
Different PLGApolymers PVA 120583m order
rhBMP2adsorption40ndash75
20ndash80 initialburst (1 day) mdash Schrier et al 2001
[57]
12 BioMed Research International
Table 2 Continued
Polymers Stabilizer Size Encapsulation EE Release Biological activity Reference
PLGAPEG PVA 37ndash67 120583m 72ndash99 33 initial burst (1day)
Little loss ofbioactivity
Lochmann et al2010 [58]
PLGAPLGA-PEG-PLGA PVA 100 120583m HSA-BMP2
6070 initial burst (1
day)No loss ofbioactivity
White et al 2013[59]
PLGA PVA 100ndash1000 nm Α-1-antitrypsin90
30 initial burst (1day)
50 after 24 days
Biological activitywas preservedusing BSA and120573-cyclodextrine
Pirooznia et al2012 [60]
promoted by the correct transfection of the delivered bio-functional BMP2-DNA [130]
In spite of the general caution with gene therapy thegenetic delivery of BMP2 has the potentiality of a better safetycompared with the delivery of large amounts of recombinantprotein [131] Lu et al specify the urgent need to developmoreefficient delivery nanoparticles and transfection methods inorder to apply the nonviral vectors in stem cell engineeringand bone regeneration Although enhanced bone formationhas been shown in several recent studies using genes suchas HIF-1120572 and miRNAs new genetic sequences will bediscovered and used in bone engineering in the near futurethat will most likely change our perspective [132]
PLGA nanospheres represent a well-studied biomoleculedelivery system that could be applied to cell targeting inorder to enhance the delivery of specific proteins or nucleicacids inside or near the bone engineering reference cells thatis mesenchymal stem cells [133]The targeting properties canbe supplied by a ligand functionalization strategy modifica-tion of the surface structure of the nanocarrier by conjugatinga cell-specific ligand to direct the release of encapsulatedbiomolecules preferably in close association with the targetcells [134]The use of pegylated nanoparticles with a covalentattachment of different ligands is reported as a potentialtechnique to deliver bone cell-specific biomolecules for boneengineering [135]
Specific antibodies that recognize surface receptors inthese cells could be covalently coupled to the surface of PLGAnanoparticles obtaining ldquoimmunonanoparticlesrdquo There areseveral examples of antibody immobilization on surfaceof PLGA nanoparticles Kocbek et al demonstrated thespecific recognition of breast tumor cells by a specific mono-clonal antibody attached on PLGA fluorescent nanoparticlesobtained by WOW emulsion process [136] For the surfacecovalent attachment they used a more simple carbodiimidemethod which promotes the formation of an amide bondbetween free carboxylic end groups of PLGA nanoparticlesand primary amine groups of the antibody molecule [81]This procedure can be highly influenced by the presenceof stabilizers frequently used to confer colloidal stabilityto nanoparticles The electrophoretic mobility of PLGAnanoparticles with an antibody (immuno-120574-globuline anti-human C-reactive protein) covalently attached on the surfaceis shown in Figure 5 It is necessary to remark the drasticdecrease in the mobility values of the antibody-modified
nanoparticles with respect to bare PLGA nanoparticleswhich could imply low colloidal stability and the subse-quent aggregation of the nanosystem Santander-Ortega etal proposed a lower antibody loading in which the barePLGA patches must be coated by a nonionic surfactant inorder to obtain immunoreactive stable nanoparticles [95]Ratzinger et al indicated that the presence of high polox-amer concentrations decreased the coupling efficiency tocarboxylic end groups in PLGA nanoparticles showing thatan equilibrium that combines sufficient stability and the bestcoupling efficiency is necessary [98] To prevent this problemCheng et al synthetized carboxyl functionalized PLGA-PEG block copolymer attaching a specific aptamer to thesurface of pegylated nanoparticles via carbodiimide methodIn this work an enhanced drug delivery to prostate tumorshas been shown in comparison to equivalent nontargetednanoparticles [137]
37 Scaffolds The data reported in the literature indicatethat PLGA micronanoparticles are promising to achievea sustained spatial and temporally controlled delivery ofgrowth factors required for cell growth and cell differen-tiation They can be incorporated with cells in solid scaf-fold or injectable hydrogels [73] Scaffolds are porous 3Dstructures normally used to improve tissue-engineered bone[28] According to Tian et al [45] a scaffold designedwith this objective must have (1) appropriate mechanicalstrength to support the growth of new bone (2) appropriateporosity to allow ingrowth of bone-related cells (3) goodbiocompatibility allowing the growth of cells on its surfacewithout being rejected by the body and (4) low toxicity tocells and tissues surrounded and (5) must be able to induceosteogenic differentiation of bone-related stem cells and (6)be biodegradable with nontoxic degradation products thatcan be eventually replaced by new bone Additionally thescaffold for bone regeneration must maintain the delivery orrelease of BMP (growth factors) ldquoin siturdquo for a long time Inthis way nanomicroparticles inside scaffolds are being usedto release an adequate flow of these signaling biomoleculesand preserve their functional structure [138] The incorpo-ration of colloidal micronanoparticles into fibrous scaffoldsadds in the possibility of multiple drugs loading Howeverthis multidrug system could also involve a decrease ofthe mechanical properties of the structure and a possibleloss of nanoparticles entrapped between the fibers [139]
BioMed Research International 13
Considering that the in vivo half-life of most biomoleculesespecially proteins is relatively short it is essential thatbioactive scaffolds maintain a desired concentration ldquoin siturdquoto direct tissue regeneration To do so an initial release ofthe encapsulated growth factor in the first hours to quicklyget an effective therapeutic concentration followed by asustained long-time release profile is required [139] Most ofthe polymeric particles inserted in scaffold structures are ina micron-scale The main objective of these microparticlesis the protection and temporary control of growth factordelivery However given the porosity of these structuresnanoparticles and especially particles of a few microns maybecome more important since it is possible to design systemswith a simple and easy diffusion through the structure Thisprocess could allow the specific recognition of a particularcell type releasing their encapsulated BMPs in the sameenvironment and helping their differentiation to cellbonetissue In any case the larger-size microspheres might notnecessarily be useless for bone regeneration scaffolds As themicrospheres gradually degrade the space they occupied willbe conducive to ingrowth of tissue In addition to affectingthe compressionmodulus of scaffolds because of their hollowfeature the particle size of microspheres can also influencethe release of rhBMP2 [45]
4 Conclusion
The use of polymeric particles using PLGA is a promisingsystem for a spatially and temporally controlled delivery ofgrowth factors that promote cell growth and differentiationin bone engineering and regeneration by means of theirincorporation beside cells into solid scaffold or hydrogels
The PLGA is widely used for its biodegradability andbiocompatibility and is approved by FDA and the EuropeanMedicines Agency for use in drug delivery systems suppliedvia parenteral On the other hand BMPs are potent growthfactors for bone repair and specifically BMP2 shows excellentability to induce bone formation of adequate quality Theprocedure for synthesizing PLGA nano- or microparticlescan be modified in their different variables to obtain systemswith controlled size in which it is possible to encapsulatehydrophobic or hydrophilic molecules with an adequate col-loidal stability and the possibility of surface functionalizationfor targeted delivery
With this scenario an optimization of methods and com-ponentsmust balance the structure andmorphology of PLGAmicronanoparticles in order to achieve high encapsulationefficiency of BMP2 and looking for a main goal control ofdelivery reducing the initial burst and reaching a sustainedrelease profile preserving the biological activity and directedto the target cells tominimize the clinical amount needed andallowing a correct bone tissue regeneration
Conflict of Interests
The authors declare no conflict of interests with any of theproducts listed in the paper
Acknowledgments
The authors wish to express their appreciation for thefinancial support granted by the ldquoMinisterio de Educaciony Cienciardquo (MEC Spain) Projects MAT2013-43922-R andResearch Groups no FQM-115 no CTS-138 and no CTS-583 (Junta de Andalucıa Spain) Partial support was alsoprovided by the Andalucıa Talent Hub Program from theAndalusian Knowledge Agency cofunded by the EuropeanUnionrsquos Seventh Framework Program Marie Skłodowska-Curie actions (COFUND Grant Agreement no 291780) andthe Ministry of Economy Innovation Science and Employ-ment of the Junta de Andalucıa (Miguel Padial-Molina)
References
[1] M Padial-Molina P Galindo-Moreno and G Avila-OrtizldquoBiomimetic ceramics in implant dentistryrdquoMinerva Biotecno-logica vol 21 no 3 pp 173ndash186 2009
[2] B Al-Nawas and E Schiegnitz ldquoAugmentation proceduresusing bone substitute materials or autogenous bonemdasha sys-tematic review and meta-analysisrdquo European Journal of OralImplantology vol 7 supplement 2 pp S219ndashS234 2014
[3] A Katranji P Fotek and H-L Wang ldquoSinus augmentationcomplications etiology and treatmentrdquo Implant Dentistry vol17 no 3 pp 339ndash349 2008
[4] C E Misch ldquoMaxillary sinus augmentation for endostealimplants organized alternative treatment plansrdquo The Interna-tional Journal of Oral Implantology Implantologist vol 4 no 2pp 49ndash58 1987
[5] C Myeroff and M Archdeacon ldquoAutogenous bone graft donorsites and techniquesrdquo The Journal of Bone amp Joint SurgerymdashAmerican Volume vol 93 no 23 pp 2227ndash2236 2011
[6] G Avila R Neiva C E Misch et al ldquoClinical and histologicoutcomes after the use of a novel allograft for maxillary sinusaugmentation a case seriesrdquo Implant Dentistry vol 19 no 4pp 330ndash341 2010
[7] S J Froum S S Wallace N Elian S C Cho and D P TarnowldquoComparison of mineralized cancellous bone allograft (Puros)and anorganic bovine bonematrix (Bio-Oss) for sinus augmen-tation histomorphometry at 26 to 32 weeks after graftingrdquoTheInternational Journal of Periodontics amp Restorative Dentistryvol 26 no 6 pp 543ndash551 2006
[8] P Galindo-Moreno G Avila J E Fernandez-Barbero et alldquoEvaluation of sinus floor elevation using a composite bone graftmixturerdquo Clinical Oral Implants Research vol 18 no 3 pp 376ndash382 2007
[9] P Galindo-Moreno I Moreno-Riestra G Avila et al ldquoEffectof anorganic bovine bone to autogenous cortical bone ratioupon bone remodeling patterns following maxillary sinusaugmentationrdquo Clinical Oral Implants Research vol 22 no 8pp 857ndash864 2011
[10] S L Wheeler ldquoSinus augmentation for dental implants theuse of alloplastic materialsrdquo Journal of Oral and MaxillofacialSurgery vol 55 no 11 pp 1287ndash1293 1997
[11] S S Wallace and S J Froum ldquoEffect of maxillary sinusaugmentation on the survival of endosseous dental implantsA systematic reviewrdquo Annals of Periodontologythe AmericanAcademy of Periodontology vol 8 no 1 pp 328ndash343 2003
14 BioMed Research International
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[13] M Padial-Molina S L Volk and H F Rios ldquoPeriostinincreases migration and proliferation of human periodontalligament fibroblasts challenged by tumor necrosis factor -alphaand Porphyromonas gingivalis lipopolysaccharidesrdquo Journal ofPeriodontal Research vol 49 no 3 pp 405ndash414 2014
[14] H Behnia A Khojasteh M Soleimani A Tehranchi and AAtashi ldquoRepair of alveolar cleft defect with mesenchymal stemcells and platelet derived growth factors a preliminary reportrdquoJournal of Cranio-Maxillofacial Surgery vol 40 no 1 pp 2ndash72012
[15] M Padial-Molina J T Marchesan A D Taut Q Jin WV Giannobile and H F Rios ldquoMethods to validate tooth-supporting regenerative therapiesrdquo Methods in Molecular Biol-ogy vol 887 pp 135ndash148 2012
[16] M R Urist ldquoBone formation by autoinductionrdquo Science vol150 no 3698 pp 893ndash899 1965
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[18] E A Wang V Rosen J S DrsquoAlessandro et al ldquoRecombinanthuman bone morphogenetic protein induces bone formationrdquoProceedings of the National Academy of Sciences of the UnitedStates of America vol 87 no 6 pp 2220ndash2224 1990
[19] J M Wozney ldquoThe bone morphogenetic protein family andosteogenesisrdquoMolecular Reproduction andDevelopment vol 32no 2 pp 160ndash167 1992
[20] E Barboza A Caula and F Machado ldquoPotential of recombi-nant human bone morphogenetic protein-2 in bone regenera-tionrdquo Implant Dentistry vol 8 no 4 pp 360ndash367 1999
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[24] K Tsuji K Cox A Bandyopadhyay B D Harfe C J Tabin andV Rosen ldquoBMP4 is dispensable for skeletogenesis and fracture-healing in the limbrdquo The Journal of Bone and Joint SurgerymdashAmerican Volume vol 90 supplement 1 pp 14ndash18 2008
[25] K Tsuji K Cox L Gamer D Graf A Economides and VRosen ldquoConditional deletion of BMP7 from the limb skeletondoes not affect bone formation or fracture repairrdquo Journal ofOrthopaedic Research vol 28 no 3 pp 384ndash389 2010
[26] G Chen C Deng and Y-P Li ldquoTGF-beta and BMP signalingin osteoblast differentiation and bone formationrdquo InternationalJournal of Biological Sciences vol 8 no 2 pp 272ndash288 2012
[27] M-C Ramel and C S Hill ldquoSpatial regulation of BMP activityrdquoFEBS Letters vol 586 no 14 pp 1929ndash1941 2012
[28] A C Carreira F H Lojudice E Halcsik R D Navarro M CSogayar and J M Granjeiro ldquoBone morphogenetic proteinsfacts challenges and future perspectivesrdquo Journal of DentalResearch vol 93 no 4 pp 335ndash345 2014
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[30] T DMueller and J Nickel ldquoPromiscuity and specificity in BMPreceptor activationrdquo FEBS Letters vol 586 no 14 pp 1846ndash1859 2012
[31] C Sieber J Kopf C Hiepen and P Knaus ldquoRecent advancesin BMP receptor signalingrdquo Cytokine amp Growth Factor Reviewsvol 20 no 5-6 pp 343ndash355 2009
[32] G Sapkota C Alarcon F M Spagnoli A H Brivanlou and JMassague ldquoBalancing BMP signaling through integrated inputsinto the Smad1 linkerrdquoMolecular Cell vol 25 no 3 pp 441ndash4542007
[33] W F McKay S M Peckham and J M Badura ldquoA comprehen-sive clinical review of recombinant human bonemorphogeneticprotein-2 (INFUSE Bone Graft)rdquo International Orthopaedicsvol 31 no 6 pp 729ndash734 2007
[34] P Hong D Boyd S D Beyea and M Bezuhly ldquoEnhancementof bone consolidation in mandibular distraction osteogenesisa contemporary review of experimental studies involving adju-vant therapiesrdquo Journal of Plastic Reconstructive and AestheticSurgery vol 66 no 7 pp 883ndash895 2013
[35] D B Spagnoli and R E Marx ldquoDental implants and the use ofrhBMP-2rdquo Dental Clinics of North America vol 55 no 4 pp883ndash907 2011
[36] M Nevins C Kirker-HeadM Nevins J AWozney R Palmerand D Graham ldquoBone formation in the goat maxillary sinusinduced by absorbable collagen sponge implants impregnatedwith recombinant human bone morphogenetic protein-2rdquo TheInternational Journal of Periodontics amp Restorative Dentistryvol 16 no 1 pp 8ndash19 1996
[37] P J Boyne L C Lilly R E Marx et al ldquoDe novo bone induc-tion by recombinant human bone morphogenetic protein-2(rhBMP-2) in maxillary sinus floor augmentationrdquo Journal ofOral and Maxillofacial Surgery vol 63 no 12 pp 1693ndash17072005
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[41] D W K Kao A Kubota M Nevins and J P Fiorellini ldquoThenegative effect of combining rhBMP-2 and Bio-Oss on boneformation for maxillary sinus augmentationrdquoThe InternationalJournal of Periodontics amp Restorative Dentistry vol 32 no 1 pp61ndash67 2012
[42] O Hanisch D N Tatakis M D Rohrer P S Wohrle JM Wozney and U M E Wikesjo ldquoBone formation andosseointegration stimulated by rhBMP-2 following subantralaugmentation procedures in nonhuman primatesrdquoThe Interna-tional Journal of Oral ampMaxillofacial Implants vol 12 no 6 pp785ndash792 1997
[43] K Wada A Niimi K Watanabe T Sawai and M UedaldquoMaxillary sinus floor augmentation in rabbits a comparative
BioMed Research International 15
histologic-histomorphometric study between rhBMP-2 andautogenous bonerdquo The International Journal of Periodontics ampRestorative Dentistry vol 21 no 3 pp 253ndash263 2001
[44] R Fu S Selph M McDonagh et al ldquoEffectiveness and harmsof recombinant human bone morphogenetic protein-2 in spinefusion a systematic review and meta-analysisrdquo Annals of Inter-nal Medicine vol 158 no 12 pp 890ndash902 2013
[45] Z Tian Y Zhu J Qiu et al ldquoSynthesis and characterization ofUPPE-PLGA-rhBMP2 scaffolds for bone regenerationrdquo Journalof Huazhong University of Science and TechnologymdashMedicalScience vol 32 no 4 pp 563ndash570 2012
[46] M Rodrıguez-Evora E Garcıa-Pizarro C del Rosario et alldquoSmurf1 knocked-down mesenchymal stem cells and BMP-2 in an electrospun system for bone regenerationrdquo Biomacro-molecules vol 15 no 4 pp 1311ndash1322 2014
[47] R Reyes A Delgado R Solis et al ldquoCartilage repair bylocal delivery of TGF-1205731 or BMP-2 from a novel segmentedpolyurethanepolylactic-co-glycolic bilayered scaffoldrdquo Journalof Biomedical Materials Research Part A 2013
[48] C V Rahman D Ben-David A Dhillon et al ldquoControlledrelease of BMP-2 from a sintered polymer scaffold enhancesbone repair in a mouse calvarial defect modelrdquo Journal of TissueEngineering and Regenerative Medicine vol 8 no 1 pp 59ndash662014
[49] Y Lupu-Haber O Pinkas S Boehm T Scheper C Kasper andM Machluf ldquoFunctionalized PLGA-doped zirconium oxideceramics for bone tissue regenerationrdquoBiomedicalMicrodevicesvol 15 no 6 pp 1055ndash1066 2013
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[51] W-G La S-W Kang H S Yang et al ldquoThe efficacy of bonemorphogenetic protein-2 depends on its mode of deliveryrdquoArtificial Organs vol 34 no 12 pp 1150ndash1153 2010
[52] Y-I Chung K-M Ahn S-H Jeon S-Y Lee J-H Lee and GTae ldquoEnhanced bone regeneration with BMP-2 loaded func-tional nanoparticle-hydrogel complexrdquo Journal of ControlledRelease vol 121 no 1-2 pp 91ndash99 2007
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[97] D-L Fang Y Chen B Xu et al ldquoDevelopment of lipid-shell andpolymer core nanoparticles with water-soluble salidroside foranti-cancer therapyrdquo International Journal ofMolecular Sciencesvol 15 no 3 pp 3373ndash3388 2014
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[99] S-S Feng and G Huang ldquoEffects of emulsifiers on thecontrolled release of paclitaxel (Taxol) from nanospheres ofbiodegradable polymersrdquo Journal of Controlled Release vol 71no 1 pp 53ndash69 2001
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[103] J S Tan D E Butterfield C L Voycheck K D Caldwell and JT Li ldquoSurfacemodification of nanoparticles by PEOPPOblock
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copolymers to minimize interactions with blood componentsand prolong blood circulation in ratsrdquo Biomaterials vol 14 no11 pp 823ndash833 1993
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[106] P Paolicelli C Prego A Sanchez and M J Alonso ldquoSurface-modified PLGA-based nanoparticles that can efficiently asso-ciate and deliver virus-like particlesrdquo Nanomedicine vol 5 no6 pp 843ndash853 2010
[107] I Brigger C Dubernet and P Couvreur ldquoNanoparticles incancer therapy and diagnosisrdquoAdvancedDrugDelivery Reviewsvol 54 no 5 pp 631ndash651 2002
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[109] E R Balmayor G A Feichtinger H S Azevedo Mvan Griensven and R L Reis ldquoStarch-poly-120576-caprolactonemicroparticles reduce the needed amount of BMP-2rdquo ClinicalOrthopaedics and Related Research vol 467 no 12 pp 3138ndash3148 2009
[110] M J Santander-Ortega D Bastos-Gonzalez J L Ortega-Vinuesa andM J Alonso ldquoInsulin-loaded PLGA nanoparticlesfor oral administration an in vitro physico-chemical character-izationrdquo Journal of Biomedical Nanotechnology vol 5 no 1 pp45ndash53 2009
[111] L Meinel O E Illi J Zapf M Malfanti H Peter Merkleand B Gander ldquoStabilizing insulin-like growth factor-I inpoly(DL-lactide-co-glycolide) microspheresrdquo Journal of Con-trolled Release vol 70 no 1-2 pp 193ndash202 2001
[112] C Srinivasan Y K Katare T Muthukumaran and A K PandaldquoEffect of additives on encapsulation efficiency stability andbioactivity of entrapped lysozyme from biodegradable polymerparticlesrdquo Journal of Microencapsulation vol 22 no 2 pp 127ndash138 2005
[113] M Tobıo S P Schwendeman Y Guo J McIver R Langerand M J Alonso ldquoImproved immunogenicity of a core-coatedtetanus toxoid delivery systemrdquoVaccine vol 18 no 7-8 pp 618ndash622 1999
[114] D K Malik S Baboota A Ahuja S Hasan and J Ali ldquoRecentadvances in protein and peptide drug delivery systemsrdquoCurrentDrug Delivery vol 4 no 2 pp 141ndash151 2007
[115] J L Cleland ldquoProtein delivery from biodegradable micro-spheresrdquo in Protein Delivery Physical Systems L M Sandersand R W Hendron Eds pp 1ndash41 Plenum Press New YorkNY USA 1997
[116] S H Oh T H Kim and J H Lee ldquoCreating growth factorgradients in three dimensional porous matrix by centrifugationand surface immobilizationrdquo Biomaterials vol 32 no 32 pp8254ndash8260 2011
[117] B N Brown J E Valentin A M Stewart-Akers G P McCabeand S F Badylak ldquoMacrophage phenotype and remodelingoutcomes in response to biologic scaffolds with and without acellular componentrdquo Biomaterials vol 30 no 8 pp 1482ndash14912009
[118] B N Brown J M Freund L Han et al ldquoComparison of threemethods for the derivation of a biologic scaffold composed ofadipose tissue extracellularmatrixrdquoTissue EngineeringmdashPart CMethods vol 17 no 4 pp 411ndash421 2011
[119] B Li T Yoshii A E Hafeman J S Nyman J C Wenkeand S A Guelcher ldquoThe effects of rhBMP-2 released frombiodegradable polyurethanemicrosphere composite scaffoldson new bone formation in rat femorardquo Biomaterials vol 30 no35 pp 6768ndash6779 2009
[120] A Paillard-Giteau V T Tran O Thomas et al ldquoEffect ofvarious additives and polymers on lysozyme release fromPLGAmicrospheres prepared by an sow emulsion techniquerdquoEuropean Journal of Pharmaceutics and Biopharmaceutics vol75 no 2 pp 128ndash136 2010
[121] B P Nair and C P Sharma ldquoPoly(lactide-co-glycolide)-laponite-F68 nanocomposite vesicles through a single-stepdouble-emulsion method for the controlled release of doxoru-bicinrdquo Langmuir vol 28 no 9 pp 4559ndash4564 2012
[122] P Yilgor N Hasirci and V Hasirci ldquoSequential BMP-2BMP-7 delivery from polyester nanocapsulesrdquo Journal of BiomedicalMaterials ResearchmdashPart A vol 93 no 2 pp 528ndash536 2010
[123] Y Xia Q Xu C-H Wang and D W Pack ldquoProtein encapsu-lation in and release from monodisperse double-wall polymermicrospheresrdquo Journal of Pharmaceutical Sciences vol 102 no5 pp 1601ndash1609 2013
[124] Y Fu L Du QWang et al ldquoIn vitro sustained release of recom-binant human bone morphogenetic protein-2 microspheresembedded in thermosensitive hydrogelsrdquo Die Pharmazie vol67 no 4 pp 299ndash303 2012
[125] D H R Kempen L Lu T E Hefferan et al ldquoRetention ofin vitro and in vivo BMP-2 bioactivities in sustained deliveryvehicles for bone tissue engineeringrdquo Biomaterials vol 29 no22 pp 3245ndash3252 2008
[126] D H R Kempen L Lu A Heijink et al ldquoEffect of localsequential VEGF andBMP-2 delivery on ectopic and orthotopicbone regenerationrdquo Biomaterials vol 30 no 14 pp 2816ndash28252009
[127] H Nie M-L Ho C-K Wang C-H Wang and Y-C FuldquoBMP-2 plasmid loaded PLGAHAp composite scaffolds fortreatment of bone defects in nude micerdquo Biomaterials vol 30no 5 pp 892ndash901 2009
[128] F Wegman Y van der Helm F C Oner W J A Dhert andJ Alblas ldquoBone morphogenetic protein-2 plasmid DNA as asubstitute for bone morphogenetic protein-2 protein in bonetissue engineeringrdquo Tissue Engineering Part A vol 19 no 23-24pp 2686ndash2692 2013
[129] J Fischer A Kolk S Wolfart et al ldquoFuture of local boneregenerationmdashprotein versus gene therapyrdquo Journal of Cranio-Maxillofacial Surgery vol 39 no 1 pp 54ndash64 2011
[130] C Qiao K Zhang H Jin et al ldquoUsing poly(lactic-co-glycolicacid) microspheres to encapsulate plasmid of bone morpho-genetic protein 2polyethylenimine nanoparticles to promotebone formation in vitro and in vivordquo International Journal ofNanomedicine vol 8 pp 2985ndash2995 2013
[131] C H Evans ldquoGene delivery to bonerdquo Advanced Drug DeliveryReviews vol 64 no 12 pp 1331ndash1340 2012
[132] C-H Lu Y-H Chang S-Y Lin K-C Li andY-CHu ldquoRecentprogresses in gene delivery-based bone tissue engineeringrdquoBiotechnology Advances vol 31 no 8 pp 1695ndash1706 2013
[133] TNVo F K Kasper andAGMikos ldquoStrategies for controlleddelivery of growth factors and cells for bone regenerationrdquo
18 BioMed Research International
Advanced Drug Delivery Reviews vol 64 no 12 pp 1292ndash13092012
[134] W Ji H Wang J J J P van den Beucken et al ldquoLocal deliveryof small and large biomolecules in craniomaxillofacial bonerdquoAdvanced Drug Delivery Reviews vol 64 no 12 pp 1152ndash11642012
[135] V Luginbuehl L Meinel H P Merkle and B Gander ldquoLocal-ized delivery of growth factors for bone repairrdquo EuropeanJournal of Pharmaceutics and Biopharmaceutics vol 58 no 2pp 197ndash208 2004
[136] P Kocbek N Obermajer M Cegnar J Kos and J Kristl ldquoTar-geting cancer cells using PLGA nanoparticles surface modifiedwith monoclonal antibodyrdquo Journal of Controlled Release vol120 no 1-2 pp 18ndash26 2007
[137] J Cheng B A Teply I Sherifi et al ldquoFormulation of func-tionalized PLGA-PEG nanoparticles for in vivo targeted drugdeliveryrdquo Biomaterials vol 28 no 5 pp 869ndash876 2007
[138] C Romagnoli F DrsquoAsta andM L Brandi ldquoDrug delivery usingcomposite scaffolds in the context of bone tissue engineeringrdquoClinical Cases inMineral and BoneMetabolism vol 10 no 3 pp155ndash161 2013
[139] D Puppi X Zhang L Yang F Chiellini X Sun and EChiellini ldquoNanomicrofibrous polymeric constructs loadedwith bioactive agents and designed for tissue engineeringapplications a reviewrdquo Journal of Biomedical Materials ResearchPart B Applied Biomaterials vol 102 no 7 pp 1562ndash1579 2014
Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595
Contents lists available at ScienceDirect
Colloids and Surfaces B Biointerfaces
jo ur nal ho me p ag e wwwelsev ier com locate co lsur fb
Full Length Article
Dual delivery nanosystem for biomolecules Formulationcharacterization and in vitro release
Inmaculada Ortega-Oller a1 Teresa del Castillo-Santaella b1 Miguel Padial-Molina aPablo Galindo-Moreno a Ana Beleacuten Joacutedar-Reyes b Joseacute Manuel Peula-Garciacutea bclowast
a Department of Oral Surgery and Implant Dentistry University of Granada Granada Spainb Biocolloid and Fluid Physics Group Department of Applied Physics University of Granada 18071 Granada Spainc Department of Applied Physics II University of Malaga 29071 Malaga Spain
a r t i c l e i n f o
Article historyReceived 25 May 2017Received in revised form 18 July 2017Accepted 17 August 2017
KeywordsPLGANanoparticlesProtein encapsulationRelease
a b s t r a c t
Because of the biocompatible and biodegradable properties of poly (lactic-co-glycolic acid) (PLGA)nanoparticles (NPs) based on this polymer have been widely studied for drugbiomolecule delivery andlong-term sustained-release In this work two different formulation methods for lysozyme-loaded PLGANPs have been developed and optimized based on the double-emulsion (wateroilwater WOW) sol-vent evaporation technique They differ mainly in the phase in which the surfactant (Pluronicreg F68) isadded water (W-F68) and oil (O-F68) The colloidal properties of these systems (morphology by SEM andSTEM hydrodynamic size by DLS and NTA electrophoretic mobility temporal stability in different mediaprotein encapsulation release and bioactivity) have been analyzed The interaction surfactant-proteindepending on the formulation procedure has been characterized by surface tension and dilatational rhe-ology Finally cellular uptake by human mesenchymal stromal cells and cytotoxicity for both systemshave been analyzed
Spherical hard NPs are made by the two methods However in one case they are monodisperse withdiameters of around 120 nm (O-F68) and in the other case a polydisperse system of NPs with diametersbetween 100 and 500 nm is found (W-F68) Protein encapsulation efficiency release and bioactivity aremaintained better by the W-F68 formulation method This multimodal system is found to be a promisingldquodual deliveryrdquo system for encapsulating hydrophilic proteins with strong biological activity at the cell-surface and cytoplasmic levels
copy 2017 Elsevier BV All rights reserved
1 Introduction
Tissue regeneration is a complex biological action involvingmultiple steps in a sequential ordered and controlled manner [12]Classically bioactive molecules have been proposed to aid in theseprocesses However the use of high doses denaturation and lossof biological activity uncontrolled timing of action and diffusionto other tissues have been highlighted as major issues of this ther-apeutic strategy [3] To help solve these problems nanomedicinehas been intensively investigated in recent years as an emerging
lowast Corresponding author at Department of Applied Physics II University of Maacutelaga29071 Maacutelaga Spain
E-mail address jmpeulaumaes (JM Peula-Garciacutea)1 Both authors contributed equally to this work
area This involves diagnostic therapeutic and regeneration meth-ods by means of structures and systems in which size and shape arecontrolled at the atomic molecular and supramolecular levels [4]The transport and controlled delivery of drugs andor therapeuticbiomolecules improve their pharmacokinetics and pharmacody-namics and at the same time minimize harmful side effects Forthese purposes different nanosystems have been described Polylactic-co-glycolic acid (PLGA) exhibits low cytotoxicity as well ashigh biocompatibility and biodegradability with the release of non-toxic by-products [5]
In the last decade the use of PLGA has been investigated todeliver a wide spectrum of active agents from hydrophobic drugmolecules [6ndash8] to hydrophilic biomolecules as peptides [9] pro-teins [10ndash15] or nucleic acids [1617] These delivery systemshave been produced via different formulation processes for theirapplication in both systemic and local site-specific therapies [18]
httpdxdoiorg101016jcolsurfb2017080270927-7765copy 2017 Elsevier BV All rights reserved
I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595 587
However their design and development as nanocarriers are diffi-cult due to the problematic release pattern when the encapsulatedmolecules are proteins for which initial bursts and slow or incom-plete release might be a problem [18ndash20] Moreover the specificconditions of the release may need to be different depending onthe final application of the nanocarrier [2021]
The water-in-oil-in-water (WOW) double emulsion techniqueis the most widely used protein-encapsulation method for PLGAmicro- (MP) and nanoparticles (NP) [2223] It allows differentfactors to be modulated such as the type of PLGA the use ofother polymers blended with PLGA the addition of surfactants themechanical stress or the organic solvent [20] It is also possible toconstruct several types of co-polymers to modify the hydropho-bicityhydrophilicity ratio [1824] and the colloidal stability sizeand release process PLGApolyethylene glycol pair and surfactantssuch as polyvinyl alcohol (PVA) or polyethylene oxides (PEO) arethe most widely studied [7122526]
On the other hand tissue engineering requires the participa-tion of mesenchymal stromal cells (MSCs) [27] MSCs are known tohave the ability to differentiate into multiple cell types includingosteoblasts Osteoblasts are the main cells responsible for synthe-sizing the mineralized compartment of bone tissue This processis regulated by among other molecules BMP-2 [3] PLGA parti-cles loaded with BMP-2 have been extensively used as has beendescribed and reviewed elsewhere [328ndash31]
Thus within this context it was the aim of the current study tooptimize the formulation and properties of a nanoparticle systemwith potential therapeutic applications Two different strategies toobtain PLGA-surfactant NPs were tested by using lysozyme as amodel for BMP-2 The size and morphology polydispersity indexzeta potential colloidal stability and encapsulation efficiency (EE)of the protein were analyzed
Once the physico-chemical characterization was completed thestudy was focused on the protein-release process using differ-ent techniques to study the results of in vitro experiments andfocusing it on the release pattern and the biological activity of thelysozyme released In this way a new formulation was establishedto develop a PLGA nanosystem with a singular dual size distribu-tion and the adequate balance between encapsulation and releaseof biologically active proteins Finally the effects of the proposedPLGA system were tested on primary MSCs in vitro as a proof ofconcept
2 Materials and methods
21 Formulation of the nanoparticles
Poly(lactide-co-glycolide) acid (PLGA 5050) ([C2 H2 O2]x [C3H4 O2]y) x = 50 y = 50 (Resomerreg 503H) 32ndash44 kDa was used asthe polymer The polymeric surfactant Pluronicreg F68 (Poloxamer188) (Sigma-Aldrich) was used as the emulsifier The structureis based on a poly(ethylene oxide)-block-poly(propylene oxide)-block-poly(ethylene oxide) and it is expressed as PEOa-PPOb-PEOawith a = 75 and b = 30 Lysozyme from chicken egg white (Sigma-L7651) was used as hydrophilic protein Water was purified ina Milli-Q Academic Millipore system Two different formulationmethods were developed termed O-F68 and W-F68
In the O-F68 method 25 mg of PLGA and 15 mg of F68 were dis-solved in 660 L of dichloromethane (DMC) and vortexed Then330 L of acetone were added and vortexed Next 100 L of abuffered solution at pH 128 with or without lysozyme (5 mgmL)were added dropwise while vortexing for 30 s Immediately thisprimary wateroil (WO) emulsion was poured into a glass con-taining 125 mL of ethanol under magnetic stirring and 125 mLof MilliQ water were added After 10 min of magnetic stirring the
organic solvents were rapidly extracted by evaporation under vac-uum until the sample reached a final volume of 10 mL
In the W-F68 method 100 mg of PLGA were dissolved in a tubecontaining 1 mL of ethyl acetate (EA) and vortexed 40 L of abuffered solution at pH 128 with or without lysozyme (20 mgmL)were added and immediately sonicated (Branson Ultrasonics 450Analog Sonifier) fixing the Duty cycle dial at 20 and the Outputcontrol dial at 4 for 1 min with the tube surrounded by ice This pri-mary WO emulsion was poured into a plastic tube containing 2 mLof a buffered solution (pH 128) of F68 at 1 mgmL and vortexingfor 30 s Then the tube surrounded by ice was sonicated again atthe maximum amplitude for the micro tip (Output control 7) for1 min This second WOW emulsion was poured into a glass con-taining 10 mL of the buffered F68 solution and kept under magneticstirring for 2 min The organic solvent was then rapidly extractedby evaporation under vacuum to a final volume of 8 mL
22 Cleaning and storage
After the organic solvent evaporation the sample was cen-trifuged for 10 min at 20 C at 14000 or 12000 rpm for O-F68 andW-F68 methods respectively The supernatant was filtered using100 nm filters for measuring the free non-encapsulated protein Thepellet was then resuspended in PB up to a final volume of 4 mL andkept under refrigeration at 4 C
221 Protein loading and encapsulation efficiencyThe initial protein loading was optimized for the nanoparticle
formulation preserving the final colloidal stability after the evapo-ration step and being different for each nanosystem Also 16 ww(LysPLGA) was used for O-F68 and 08 ww (LysPLGA) for W-F68one The amount of encapsulated lysozyme was calculated by mea-suring the difference between the initial amount added and the freenon-encapsulated protein which was tested by bicinchoninic acidassay (BCA Sigma-Aldrich) Then protein encapsulation efficiency(EE) and final drug loading (DL) was calculated as follows
EE = MI minus MF
MItimes 100 DL = MI minus MF
Mpolymertimes 100
where MI the initial total mass of Lys MF is the total mass of Lys inthe aqueous supernatant and Mpolymer is the mass of PLGA in theformulation
23 Characterization of the nanoparticles
231 Interfacial characterization of the first water-in-oilemulsion
The surface tension and dilatational rheology measurementsat the air-water interface were made in the OCTOPUS [32] aPendant Drop Surface Film Balance equipped with a subphasemulti-exchange device (patent submitted P201001588) describedin detail elsewhere [33] Here air plays the role of the organic phaseThe surface tension is calculated with DINATENreg software basedon axisymmetric drop shape analysis (ADSA) and the dilatationalmodulus (E) of the interfacial layer is determined from image anal-ysis with the program CONTACTOreg The in vitro model is describedin ldquoSupplementary materialrdquo
232 Particle morphologyNanoparticles were imaged by Scanning Electron Microscopy
(SEM) and Scanning Transmission Electron Microscopy (STEM)using a Zeiss SUPRA 40VP field emission scanning electron micro-scope from the Centre for Scientific Instrumentation of theUniversity of Granada (CIC UGR)
588 I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595
233 Nanoparticle size and electrokinetic mobilityThe hydrodynamic diameter and electrophoretic mobility of the
NPs were determined by using a Zetasizer NanoZeta ZS device(Malvern Instrument Ltd UK) working at 25 C with a He-Ne laserof 633 nm and a scattering angle of 173 Each data point wastaken as an average over three independent sample measurementsThe size of the NPs was characterized by Dynamic Light Scattering(DLS) The average hydrodynamic diameter (Z-average or cumu-lant mean) and the polydispersity index (PDI) were computedThese parameters are calculated through a cumulant analysis ofthe data which is applicable for narrow monomodal size distribu-tions [34] We also determined the intensity size distribution froman algorithm provided by the Zetasizer software (General Purpose)
The electrophoretic mobility was determined by the techniqueof Laser Doppler Electrophoresis An electrophoretic mobility dis-tribution as well as an average electrophoretic mobility (-average)was established for each sample
The hydrodynamic size distribution of the NPs with wide sizedistributions from DLS was also measured by using Nanoparti-cle Tracking Analysis (NTA) in a NanoSight LM10-HS(GB) FT14(NanoSight Amesbury United Kingdom) All samples were mea-sured more than three times for 60 s with manual shutter gainbrightness and threshold adjustments at 25 C The average sizedistribution (particle concentration vs diameter) was calculatedas an average of at least three independent size distributions
234 Nuclear magnetic resonance (NMR) of the nanoparticlesThe 1HNMR spectra of free F68 lysozyme-loaded particles from
O-F68 method with and without F68 and lysozyme-loaded parti-cles from W-F68 method were measured with a VNMRS 500 MHzspectrometer (Agilent) in the Centre for Scientific Instrumentation(CIC) of the University of Granada
24 Colloidal and temporal stability in biological media
The average hydrodynamic diameter and the polydispersityindex (PDI) by DLS of each system were measured to determinetheir colloidal stability in different media (Phosphate buffer [PB]Phosphate buffer saline [PBS] and cell culture medium Dulbeccorsquosmodified Eaglersquos medium [DMEM] from Sigma) and at differenttimes after (0 1 and 5 days)
In vitro release experiments were conducted following a simi-lar methodology as described above (Encapsulation efficiency) butusing 1 mL of each sample suspended in PBS at 37 C The proteinreleased from these samples was determined every 24 h by super-natant analysis and the pellet was suspended in the same volumeof buffer to maintain the release conditions All experiments weredeveloped in triplicate
241 Confocal microscopyLysozyme was labeled with fluorescein isothiocyanate (FITC)
using a method described by Kok et al [35] After FITC and lysozymecovalent conjugation concentrations were estimated spectropho-tometrically using the extinction coefficients described for FITC at494 nm and 280 nm The lysozyme concentration was calculatedmeasuring optical absorbance at 280 nm and subtracting the cor-responding FITC absorbance at this wavelength Images were madein a Nikon A1 laser scanning confocal microscope from CIC UGRAll experiments were performed in triplicate and replicated at leasttwice
25 Biological activity and interactions
251 Lysozyme biological activityThe biological activity of lysozyme was analyzed by an enzy-
matic activity kit (Sigma-Aldrich) using Micrococcus lysodeikticuscells as the substrate following the manufacturerrsquos instructions
252 Cellular uptakePrimary human mesenchymal stem cells (hMSCs) were taken
from healthy maxillary alveolar bone according to previouslydescribed protocols [36] After confirming their phenotype byflow cytometry and trilineage differentiation tests 12000 cellsper well were cultivated in sterile plates with glass bottom(Ibidi cat n 81158) overnight These cells were treated withmedium without fetal bovine serum (FBS) and Cell Tracker Red(15000) (C34552 ThermoFisher) for 30 min Then the mediumwas removed and supplemented with 10 FBS after which theparticles with lysozyme-FITC were added Then the hMSCs wereincubated 30 min again washed three times with PBS 1X and freshmedium supplemented with 2 FBS added Finally the hMSCs wereexamined by a confocal microscope (Nikon Eclipse Ti-E) Cell cul-tures were in all cases maintained at 37 C and 5 CO2 atmosphere
3 Results and discussion
31 Formulation of the nanoparticles
The methods developed in this work are intended to improvethe existing formulation techniques for hydrophilic protein loaded-PLGA NPs based on a double-emulsion process [1022] The noveltyof these methods is the use of the polymeric surfactant F68 either inthe organic phase (O-F68 method) or in the aqueous phase (W-F68)This surfactant reduces the size of the NPs enhances their stabilityand protects the encapsulated protein In addition the presence ofF68 on the surface of the particles reduces the recognition of thenanocarriers by the mononuclear phagocytic system (MPS) [37]
Additionally the choice of the organic solvent significantlyaffects the properties of the final colloidal system since the organicsolvent solubility regulates the inner and surface structure of theparticle In addition the interaction of the solvent with the encap-sulated biomolecule can alter its bioactivity as a consequence ofits denaturation as found for methylene chloride [26] In the O-F68method DMC is chosen as the organic solvent due to its lower watersolubility to facilitate the emulsification process and its low boil-ing point for easy evaporation However a freely water-miscibleorganic solvent (acetone) and the emulsifier F68 were added inthis organic phase to reduce its negative biological effects on theencapsulated protein [24] This emulsifier also reduces the protein-hydrophobic PLGA matrix interaction and thus the disruption ofthe protein structure [3] By contrast in the W-F68 method ethylacetate was used as the organic solvent which exerts less denatur-izing effects on the encapsulated protein [38] The higher watersolubility of this solvent favors rapid solvent removal The sol-vent removal rate is also accelerated by increasing the shear stressduring the second emulsification step It also enhances the encap-sulation efficiency and minimizes the contact time between theprotein and organic solvent [3] Poloxamer F68 is introduced in theexternal aqueous phase
Both formulations (O-F68 and W-F68) (Table 1) gave rise to col-loidally stable samples and the encapsulation of lysozyme insidethe nanoparticles in agreement with the double WOW emulsionmethod [23] Lysozyme was chosen as a model protein due to itsbiostability well-known characteristics and ease in quantifying itsbiological activity [3940] In addition its molecular size (143 kD)and its basic isoelectric point (around pH = 11) make it an appro-
I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595 589
Table 1Formulation conditions and protein encapsulation results PLGA F68 and LYSI are the initial amount of polymer surfactant and lysozyme respectively Initial is the initialpolymer-protein rate in ww EE is the encapsulation efficiency LYSF is the final encapsulated amount of lysozyme DL is the final drug loading rate in ww
PLGA (mg) F68 (mg) LYSI (mg) Initial EE LYSF (mg) DL
O-F68-Lys 25 15 04 16 625 025 1W-F68-Lys 100 2 08 08 731 058 058
priate model for other proteins such as bone-growth factors [15]Three main objectives drove the optimization of the appropriaterelation among the polymer poloxamer and protein (1) to havecolloidally stable nanosystems of submicron sizes (2) to encap-sulate a sufficient amount of protein and (3) to prevent proteindestabilization by maintaining their biological activity
Therefore regardless of the formulation method it wasintended to limit the initial protein loading to provide colloidallystable nanosystems In our case as shown in Table 1 Initial val-ues were the best choice to maintain colloidal stability withoutsignificantly changing the size distribution (see below) In con-sequence DL presents relative low values for both formulationsalthough the encapsulated amount of lysozyme LYSF is greaterthan those required for therapeutic proteins with lower clinicallyeffective amounts [41] The value of EE found for O-F68-Lys NPsis in consonance with the formulation characteristics and simi-lar to other reports with different proteins [12104214] includingbovine serum albumin (BSA) or insulin [1242] and several growthfactors [14]
The presence of surfactant stabilizes the emulsion droplets andreduces their size However it also alters the protein-polymerinteraction which translates into a reduction of the encapsulationefficiency This was evidenced by Blanco et al when encapsulat-ing BSA and lysozyme in different PLGA-poloxamer microparticles[10] Moreover the type of protein and its initial theoretical loadingare factors directly related with the EE and can affect the colloidalstability of the primary emulsion as shown by Santander et al [12]The different polymersurfactant ratio between the two formula-tions is not comparable since the surfactant is added in a differentway In both cases we used previous formulations as the startingpoint [1022] and tested several polymersurfactant ratios (datanot shown) in order to obtain the best colloidal stability EE andDL In Table 1 we show the data for the optimized PLGAF68 ratiosin both systems
In the W-F68 method despite the higher EE value with respectto O-F68 system an almost complete encapsulation was expecteddue to the low initial proteinPLGA mass ratio [12] and to theabsence of surfactant in the first emulsion step The characteris-tics of the modified formulation process may have the key In thisformulation the relatively high solubility of the ethyl acetate inwater promotes rapid diffusion of the organic solvent into the sec-ond aqueous phase An initial small volume of water containingpoloxamer is initially added to prevent a rapid uncontrolled precip-itation of the polymer and to control the speed of the process Thisis subsequently supplemented with the addition of a larger aque-ous volume as previously described [26] When this solidificationis slow it favors the escape of the protein and the EE decreasesHowever if the solidification is very fast the contact of proteinwith the organic solvent is minimized and the EE increases On thenegative side it can produce polymer agglomeration which inter-feres with the correct formation of the NPs The introduction of anintermediate step with a reduced volume of aqueous phase withpoloxamer can modulate the rate of the process by controlling thediffusion of ethyl acetate into the water and by allowing the diffu-sion into the organic phase of the poloxamer A controlled velocityof the polymer pre-solidification process in the presence of surfac-tant can produce channels or pores in the polymeric shell that onone hand could facilitate the protein release and on the other hand
could drive down the EE value [43] As a result of these phenom-ena the final DLs (ww of lysozymepolymer) shown in Table 1 forboth NP systems are suitable for their application as nanotransportsystems
32 Characterization of the nanoparticles
321 Interfacial characterization of the first water-in- oilemulsion
To gain better insight into the effect of the formulation methodon the interfacial properties of the first water (lysozyme solution)-in-oil emulsion we designed surface experiments with lysozymeand Pluronicreg F68 The main difference in the two formulationmethods is how the Pluronicreg F68 is added in aqueous phase(WndashF68) or in organic phase (O-F68) This difference could affectthe composition of the surface of the NPs and as a result theircolloidal properties
The surface tension and elasticity at the air-water interfacewere the properties analyzed (Table 2) At this interface proteinschange their conformation and expose their hydrophobic part toair depending on their thermodynamic stability flexibility amphi-pathicity molecular size and charge In our case lysozyme is aglobular protein that is adsorbed at the air-water interface andforms a rigid monolayer due to its internal structure and the pres-ence and number of disulfide bridges [44] Our measurements weremade at pH 12 thus lysozyme is negatively charged Table 2 showsthe interfacial tension of the lysozyme monolayer at the air-waterinterface after 50 min of adsorption (457 plusmn 04 (mNm)) and itselasticity (83 plusmn 4 (mNm)) The reduction of the interfacial tensionwhen compared with that of the air-water interface (72 mNm)indicates the surfactant characteristics of the lysozyme The highvalue of elasticity was due to the charge and high molecular inter-actions in the lysozyme monolayer When the monolayer is formedwith Pluronicreg F68 the surface tension is slightly lower than withlysozyme when the Pluronicreg is added in AP but similar (takinginto account the error) when added in OP
Pluronicreg F68 is an amphiphilic molecule that is adsorbed at theair-water interface when it is dissolved in aqueous phase and alsowhen it is deposited onto the surface of the drop Small differencesare found when comparing the surface tension of the Pluronicreg
monolayer from the two methods The different values of interfa-cial tension attained in both cases would be due to the differentmethods to add the Pluronicreg F68 at the formed lysozyme mono-layer Pluronicreg F68 presents lower elasticity than the lysozyme asexpected since Pluronicreg F68 is known to form a flexible monolayerat the air-water interface [45]
Two assays were designed to mimic the formulation methodsof the particles In the first assay (W-F68 method) a monolayer oflysozyme was formed then the bulk of the drop was exchangedwith the aqueous solution of Pluronicreg F68 and after adsorptionthe interfacial tension and elasticity of the interface were mea-sured (379 plusmn 06 mNm and 142 plusmn 05 mNm respectively) Thislow value of elasticity was very similar to that of the monolayerof Pluronicreg F68 indicating that Pluronicreg F68 is located at theinterface and removes the previously adsorbed lysozyme In thesecond assay (O-F68 method) after the monolayer of lysozyme wasformed the Pluronicreg F68 dissolved in chloroform is deposited ontothe surface of the drop The chloroform is rapidly evaporated and
590 I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595
Table 2Interfacial tension and dilatational elasticity (at 1 Hz) of the air-water interface (a) after adsorbing lysozyme or Pluronicreg F68 in the aqueous phase (AP) or Pluronicreg F68 inorganic phase (OP) in the first step (b) when Pluronicreg F68 is added in AP or OP after adsorption of lysozyme monolayer (mean plusmn sd n = 3)
First step Interfacial Tension(mNm)
Elasticitya
(mNm)Second step Interfacial Tension
(mNm)Elasticityb
(mNm)
Lysozyme 457 plusmn 04 83 plusmn 4 Pluronicreg F68 (AP) 379 plusmn 06 142 plusmn 05Lysozyme 457 plusmn 04 83 plusmn 4 Pluronicreg F68 (OP) 38 plusmn 2 43 plusmn 4Pluronicreg F68 (AP) 421 plusmn 03 15 plusmn 3Pluronicreg F68 (OP) 475 plusmn 21 94 plusmn 05
the interfacial tension and elasticity of the interface are measured(38 plusmn 2 mNm and 43 plusmn 4 mNm respectively) The elasticity washalf of that of the pure lysozyme monolayer perhaps because ofthe coexistence of lysozyme and Pluronicreg F68 molecules at theinterface The surface tension of the final interface does not dependon the method of adding the Pluronicreg but it is lower than that ofthe pure lysozyme or the pure Pluronicreg
Within this context it has been widely reported that the adsorp-tion of PEO and poloxamers at the interface reduces the proteinbinding [4647] In the O-F68 method the lysozyme is exposedto the DCM after the formation of the first water-in-oil emulsioneven if Pluronicreg is added as they both coexist at the interface Inthe W-F68 method protein will be in contact with ethyl acetate inthis step as Pluronicreg is absent However this solvent has weakerbiological effects on lysozyme Pluronicreg could reach the interfacewhen added to the aqueous phase in the following step and dis-place the protein from the interface which could diffuse outwardsto the aqueous phase
322 Particle morphologyThe delivery biodistribution and action mechanism of a trans-
ported drug or biomolecule depend heavily on the size of theparticle concentration and timing [48] In general the micromet-ric scale is designed for a local supply that allows the formation ofreservoirs of the transported molecule and minimizes the actionof the phagocytic system [49] However nanometric systems aremore versatile because they permit a systemic distribution aremore stable and reactive and allow extra- as well as intracellu-lar action This latter mechanism is essential when the moleculeor drug should act in the cytoplasm [50] or any other intracellularstructure such as the mitochondria Golgi apparatus endoplasmicreticulum or nucleus [485152] Other parameters to alter the intra-cellular fate of the particles have also been investigated mainly byaltering their surface decoration [53] for example with nuclearlocalization signals (NLS) that use the nucleus as the target of theparticle [51] However these strategies are still in their very earlydevelopmental phase [4852]
A particle size in the submicron scale (between 2 and 500 nm)was sought as it is necessary for cell internalization and a rapiddistribution after parenteral administration in order to reach dif-ferent tissues through different biological barriers Particles under200 nm minimize their intake by macrophages The type of organicsolvent the polymer concentration the addition of surfactant andthe emulsification energy control the size of the system
The O-F68 method gives rise to a monomodal particle-size dis-tribution with diameters around 100 nm The addition of Pluronicreg
F68 in the organic phase bolsters colloidal stability of the first emul-sion and reduces the particle size in comparison with PLGA NPsin which the stability is purely electrostatic due to the carboxylicgroups of the PLGA In the W-F68 method shear stress and volumeof the aqueous phase are taken into account to produce a systemwith particles of between 100 and 500 nm
O-F68-Lys NPs have a spherical shape with a monomodal sizedistribution (diameters around 100 nm) and core-shell structure(Fig 1a) Empty particles produced with the O-F68 method are
shown in Figs S1 (without F68) and S2 (with F68) They are alsospherical and with a core-shell structure but slightly larger
W-F68-Lys NPs also present a spherical shape but a multi-modal size distribution with diameters between 140 and 450 nmthe largest population being around 260 nm (Fig 1b) A core-shellstructure is also observed in these particles Empty particles fromthe W-F68 method are presented in Fig S3 corresponding to a morepolydisperse system
323 Nanoparticle size electrokinetic mobility and colloidalstability
The hydrodynamic diameter distribution of the particles wasdetermined firstly by DLS Table 3 contains the main colloidal prop-erties of particles produced with the O-F68 and W-F68 methodsempty or loaded with lysozyme The results of empty particles fromthe O-F68 method but synthesized without F68 are also included
The size parameters were calculated through a cumulative anal-ysis of the data which is applicable for narrow monomodal sizedistributions [34] SEM and STEM micrographs indicate that suchan approximation could be assumed for particles from the O-F68method but not from the W-F68 one Thus the intensity size distri-butions of the different systems are shown in Fig 2a The presenceof Pluronicreg F68 in the O-F68 method significantly reduces the sizeand polydispersity of the NPs This agrees with the reduction of thesurface tension when the F68 is at the interface (Table 2) whichpromotes the emulsification process If the NPs are also loaded withlysozyme the size is even smaller but the polydispersity increasesslightly compared with the empty particles The surfactant prop-erties of the lysozyme have been shown with the surface-tensionresults (Table 2)
Fig 2a indicates the presence of particles higher than 500 nmwith the W-F68 which does not correlate with the SEM micro-graphs Thus a different technique (NTA) was used to gaininformation on the size distribution of such systems (Fig 3b) WithNTA the size distribution was consistent with the SEM imagesBroad size distributions corresponding to multimodal systemswere found with this method but the addition of lysozyme ledto a clear size reduction This is because lysozyme also acts as anemulsifier in the first emulsion
The electrokinetic charge of the NPs was analyzed by measuringthe electrophoretic mobility For comparison all the samples weremeasured at pH 7 (phosphate buffer) In Fig 3 the electrophoreticmobility distributions are presented while the corresponding -averages are shown in Table 3
PLGA NPs are usually negatively charged due to the carboxylicgroups of the polymer The use of Pluronicreg F68 in the O-F68method clearly reduces the electrophoretic mobility of the NPswhich indicates that some Pluronicreg is located at the NP sur-face This reduction was expected after the incorporation of thisnon-ionic surfactant onto the interface since the presence ofpolyethylene oxide chains would cause an outward shift of theshear plane where the -potential is defined and this would sub-sequently diminish electrophoretic mobility Previous results forPLGA particles have shown a significant reduction directly relatedto the poloxamer coating [54] If we compare the two systems the
I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595 591
Fig 1 SEM and STEM micrographs of lysozyme-loaded particles using O-F68 (a) or W-F68 method (b)
Table 3Colloidal properties of PLGA NPs from different formulation methods They were measured in phosphate buffer (pH 7) The average hydrodynamic diameter (Z-average orcumulative mean) and the polydispersity index (PDI) are determined from DLS (Mean plusmn sd n = 3)
Z-average (nm) PDI -average (mcmVs)
O-F68 method Empty without F68 266 plusmn 7 0293 minus506 plusmn 015Empty 1627 plusmn 21 0081 minus429 plusmn 018Lysozyme-loaded 1210 plusmn 12 0244 minus334 plusmn 007
W-F68 method Empty 273 plusmn 3 0193 minus531 plusmn 011Lysozyme-loaded 293 plusmn 4 0169 minus4212 plusmn 0013
Fig 2 Hydrodynamic diameter distribution (a) by DLS at pH 7 (phosphate buffer) of empty and lysozyme-loaded PLGA particles from the O-F68 and W-F68 methods and(b) by NTA at pH 7 (phosphate buffer) of empty and lysozyme-loaded PLGA particles from the W-F68 method
less negative surface for OF68 NPs would be related to less densityof surface PLGA polymer bringing the negative electrical charge tothe interface This result would be in line with the greater amountof PLGA in the formulation of WF68 nanosystem
When the lysozyme is also used in the synthesis the surfaceis even less negative which could be explained by the presence
of some protein (whose net charge is positive) near or at theinterface This latter effect is also found with the W-F68 methodThe attractive electrostatic interaction between negative terminalacid residues of PLGA and lysozyme molecules plays a key rolein the process of protein encapsulation [41] or adsorption [40]in PLGA NPs which affects the final protein loading In relation
592 I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595
Fig 3 Electrophoretic mobility distribution at pH 7 (phosphate buffer) of empty and lysozyme-loaded PLGA particles from the (a) O-F68 and (b) W-F68 methods
to this situation an important characteristic of the W-F68 encap-sulation formulation is that the water phase is at pH 12 whichallows a negative net charge of lysozyme and thus avoids theelectrostatic protein-polymer attraction This situation can reducethe encapsulation efficiency but at the same time favors the laterprotein-diffusion process and consequently the short-term release
Recent studies have proposed the use of nanoparticles embed-ded in predesigned 3D-printed scaffolds [5556] moving us toanalyze the stability of the two formulations in several mediausually employed during the preparation of other structures Sizedistributions similar to the original were found for the two formula-tions in different media (PB PBS and DMEM) and at different timesafter synthesis (0 1 and 5 days) The electric charge of PLGA acidend groups and the poloxamer molecules located on the NP sur-face confers a combined electrostatic and steric colloidal-stabilitymechanism as has previously been described [4654] Additionallythe NPs in all cases keep their size under storage at 4 C at least for1 month (data not shown) Thus the media described could poten-tially be used as storage media or to prepare other solutions orscaffolds before actually placing them in the living environment(in vitro or in vivo)
324 NMR of the nanoparticlesIn Fig 3 both empty and protein-loaded NPs present less neg-
ative electrophoretic mobility than do empty NPs without F68which could be explained by the presence of Pluronicreg F68 atthe surface of the NP By comparing the 1HNMR spectra of freePluronicreg F68 and lysozyme-loaded NPs from O-F68 and W-F68methods we can check the presence of F68 at the surface of theNPs (Fig S4) by the peaks shown between 325 and 375 ppm and at1 ppm These peaks are also visible in the spectra of NPs formulatedwith F68 (O-F68 and W-F68 Figs S5 and S6 respectively)
33 Biological activity and interactions
A controlled release from a PLGA-based delivery system is adifficult task as it depends on multiple factors the type of PLGAsolvent mechanical stress use of surfactants etc [57] The diffusionof the protein and the polymer erosion are the main mechanismsinvolved in the protein release in PLGA-based delivery systems Fur-thermore it is typical to find a rapid burst release at the initial stagefollowed by a slow release phase over the short and medium termIn this phase protein molecules diffuse through the polymer matrixuntil reaching a final phase in which the polymer degradation byhydrolysis allows a faster release [20]
On the other hand the short-term release is of special inter-est for transporting bone morphogenetic growth factors (BMPs) Acontrolled initial burst followed by a sustained release significantlyimproves in vivo regeneration of bone [3] and cartilage [58] even in
Fig 4 Cumulative release (filled symbols) and residual bioactivity (open symbols)of O-F68-Lys (square) and W-F68-Lys (triangle) incubated for different times at 37 Cin saline phosphate buffer (pH 74) (mean plusmn sd n = 3)
dual-controlled release systems [59] For these reasons we focusedour analysis on short-term release taking into account the reducedpolymer degradation by hydrolysis found for similar systems forthese early steps [60]
Fig 4 shows the accumulative release of lysozyme from O-F68-Lys NPs over the short term (seven days) These results areconsistent with a two-stepped process an initial burst and a slow-release phase The first step could correspond to the release ofthe protein molecules located near surface whose presence wasdeduced from the electrophoretic mobility results (Fig 3) The sec-ond part of the release process was limited and slow due to theprotein diffusion through the matrix of the polymeric shell Thespecific electrostatic interaction between the positive lysozymemolecules and the PLGA negative terminal acid groups can reducethe protein diffusion [10] When the poloxamer (F68) is added theinteraction between the surfactant and the protein helps the diffu-sion process leading to a more complete and sustained release [12]It also helps to keep the biological activity of the protein [4161] Thepoloxamer reduces the non-specific protein-polymer interactions(ie hydrophobic interactions) but not the specific ones (electro-statics) thus the diffusion through water-filled pores or throughthe polymer is still limited In the current study the protein fractionreleased and the release pattern are similar to those found in theliterature for lysozyme encapsulated in nano- and microparticlesof blends of PLGA and other polymers or surfactants [261511]
The protein release curve from W-F68-Lys NPs (Fig 4) revealsthat the initial delivery rate is identical to that of the O-F68 systemwhich could mean a similar proportion of encapsulated proteinclose to or at the surface for both NP systems This would agreewith the analogous decrease in the electrophoretic mobility of the
I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595 593
Fig 5 z-projection of 5 images of hMSCs visualized 30 min after incubation with W-F68-LysFITC NPs or O-F68-LysFITC NPs hMSCs were previously labeled with cell-trackerred Scale bar 20 m
lysozyme-loaded NPs previously reported (Fig 3) In the secondpart of the process the specific interaction between the proteinand the polymer is again present However the diffusion processin the W-F68 system appears to be enhanced allowing a contin-uous and sustained release after the initial burst and reaching aslightly higher value for the maximum release time studied Thisresult could be related to the inner structure of the polymer layerthat allows better hydration and therefore better diffusion of theprotein towards the outside It has been previously reported thatthe use of less polar organic solvents such as DCM for PLGA par-ticles formulations increases the density of the polymer matrix incomparison with more polar organic solvents such as EA The PLGAmatrices prove more resistant in the first case but reducing at thesame time their connectivity and diffusivity [62] Meng et al [26]found that faster removal of EA results in a slower kinetic release ofthe protein due to a decrease in the porosity of the NPs Regardingthe role of the Pluronicreg Rafati et al [63] found a higher concentra-tion of protein encapsulated in the surface pores in microparticlessynthesized in the presence of surfactant in the second aqueousphase of the emulsion Since an intermediate step was introducedin our W-F68 formulation in the second aqueous phase of the emul-sion the removal of the EA by diffusion was strongly controlled sothat it was expected that the porosity of these NPs would increaseThis porosity improves protein diffusion which allows a more sta-ble release pattern according to the experimental result found forthis system Despite the unfavorable effect of the specific electro-static protein-polymer interaction on the release the amount ofreleased protein in our NPs is substantial signifying that there areother unspecific interactions that can be modulated by the pres-ence of surfactant allowing a sustained release The amount ofreleased lysozyme is similar to that found with lysozyme physi-cally adsorbed onto the surface of PLGA nanoparticles despite theelectrostatic attraction [40] Besides other unspecific interactionsthe electrolyte concentration in the release medium could modu-late this electrostatic attraction between the protein and polymerdiminishing it and facilitating the release process [46]
Another remarkable parameter is the biological activity of thein vitro release of lysozyme shown in Fig 4 While in the O-F68system the bioactivity is partially reduced by up to 40 the pro-
tein supplied by the W-F68 system maintains the activity above90 with respect to that of commercially supplied lysozyme andresuspended in the same release buffer As discussed above boththe organic solvent and the hydrophobic interaction between theprotein and the polymer often cause denaturation of encapsulatedproteins [4164] Perez et al [11] describe a partial loss of activ-ity when using DCM and an aqueous PVA solution in the secondemulsification step without any additional excipient The use ofpoloxamers in the formulation reduces such interactions enhancesthe stability of the protein and maintains an aqueous layer thatretains the water molecules necessary for the biological functionof the protein at the same time aiding its diffusion This situationtogether with the use of a weak organic solvent such as EA helpspreserve the biological activity of the lysozyme as found for theW-F68-Lys system
Fig S7 presents different confocal microscopy images relatedto the release process of lysozyme-loaded W-F68 NPs A decreasein fluorescence intensity was appreciable over the course of thein vitro experiment In addition the aggregation of the system isvisible as the incubation process progresses The analysis of theseimages is consistent with the previously reported results for thisNP system
331 Cellular uptakeCellular uptake of PLGA NPs is a known process affected
mainly by surface properties and functionalization [9] and parti-cle aggregation [65] Internalization and subsequent intracellularprocessing of the particles have been described as an activeprocess thus it is energy dependent and can therefore beaffected by other factors that alter the energy uptake by cellssuch as temperature [48] Particles can be internalized by sev-eral endocytosis methods dependent primarily on the size ofthe particle caveolin-dependent particles (diameter asymp 60 nm)clathrin-independent (diameter asymp 90 nm) and clathrin-dependent(diameter asymp 120 nm) [5152] Once internalized about 65 areexported back to the extracellular space before releasing anyof their content while the rest slowly release the encapsulatedmolecule into the intracellular space [66] The intracellular releaseprocess is affected by the formulation of the particles [48] We have
594 I Ortega-Oller et al Colloids and Surfaces B Biointerfaces 159 (2017) 586ndash595
demonstrated that the proposed systems follow a pattern similar toothers previously published As early as 30 min after incubation W-F68-LysFITC NPs were taken up by the cells (Fig 5) Some W-F68particles were still in the medium so that the dual activity couldhappen In contrast O-F68-LysFITC NPs were affected by aggrega-tion and therefore did not properly reach the intracellular space(Fig 5 for z-axis images view Fig S8) This contradicts the previousanalyses of the colloidal stability in PB PBS and DMEM This findingcan be explained by the fact that although the culture media wasDMEM this latter medium was supplemented with fetal bovineserum and cells release many factors to the extracellular mediumthat can affect these types of particles None of the systems wereshown to be toxic for the cells (Fig S9) No studies available havereported any effects of lysozyme on hMSCs
4 Conclusions
A novel dual-delivery PLGA-nanosystem has been developedin which the formulation and components favor an adequateshort-term delivery pattern while preserving the bioactivity ofencapsulated molecules The analysis of the polymer-surfactant-protein interaction shows that the organic solvent use ofsurfactant volume relation of both phases and the net charge of theprotein play important roles in the final characteristics and releasebehavior of the nanoparticles The W-F68 formulation balances allof them in order to provide a nanosystem ready to transport anddeliver hydrophilic biomolecules such as proteins In vitro releaseexperiments display an adequate short-term delivery pattern thatat the same time preserves the bioactivity of the encapsulatedbiomolecule Additionally the singular nanoparticle size distribu-tion found for this W-F68 nanosystem allows the possibility of adual outer- and intra-cellular protein delivery as has been shownby in vitro cellular experiments This novel formulation will be usedin future studies to encapsulate and deliver growth factors in vitroand in vivo in order to exploit the therapeutic potential of thisnanosystem
Acknowledgements
The authors wish to express their appreciation for the tech-nical support to Dr Azahara Rata-Aguilar and for the financialsupport granted by the Consejeriacutea de Economiacutea Innovacioacuten Cienciay Empleo de la Junta de Andaluciacutea (Spain) through research groupsFQM-115 and CTS-1028 and by the following research projectMAT2013-43922-R ndash European FEDER support included minus (MICINNSpain)
Appendix A Supplementary data
Supplementary data associated with this article can be found inthe online version at httpdxdoiorg101016jcolsurfb201708027
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[3] Bone regeneration from PLGA micro-Nanoparticles Biomed Res Int (2015)httpwwwhindawicomjournalsbmriaa415289
[4] K-B Lee A Solanki J Kim J Jung Nanomedicine dynamic integration ofnanotechnology with biomedical science in Handb Clin Nanomedicine PanStanford 2016 2017 pp 21ndash60 httpdxdoiorg101201b19915-4
[5] GEJ Poinern A Laboratory Course in Nanoscience and Nanotechnology CRCPress Taylor amp Francis Group 2015
[6] MM Yallapu BK Gupta M Jaggi SC Chauhan Fabrication of curcuminencapsulated PLGA nanoparticles for improved therapeutic effects inmetastatic cancer cells J Colloid Interface Sci 351 (2010) 19ndash29 httpdxdoiorg101016jjcis201005022
[7] BP Nair CP Sharma Poly(lactide-co-glycolide)-laponite-F68 nanocompositevesicles through a single-step double-emulsion method for the controlledrelease of doxorubicin Langmuir 28 (2012) 4559ndash4564 httpdxdoiorg101021la300005c
[8] R Shankarayan S Kumar P Mishra Differential permeation ofpiroxicam-loaded PLGA micronanoparticles and their in vitro enhancementJ Nanopart Res 15 (2013) 1496 httpdxdoiorg101007s11051-013-1496-6
[9] JA Loureiro B Gomes G Fricker MAN Coelho S Rocha MC PereiraCellular uptake of PLGA nanoparticles targeted with anti-amyloid andanti-transferrin receptor antibodies for Alzheimerrsquos disease treatmentColloids Surf B Biointerfaces 145 (2016) 8ndash13 httpdxdoiorg101016jcolsurfb201604041
[10] D Blanco MJ Alonso Protein encapsulation and release frompoly(lactide-co-glycolide) microspheres effect of the protein and polymerproperties and of the co- encapsulation of surfactants Eur J PharmBiopharm 45 (1998) 285ndash294 httpdxdoiorg101016S0939-6411(98)00011-3
[11] C Peacuterez P De Jesuacutes K Griebenow Preservation of lysozyme structure andfunction upon encapsulation and release from poly(lactic-co-glycolic) acidmicrospheres prepared by the water-in-oil-in-water method Int J Pharm248 (2002) 193ndash206 httpdxdoiorg101016S0378-5173(02)00435-0
[12] MJ Santander-Ortega N Csaba L Gonzaacutelez D Bastos-Gonzaacutelez JLOrtega-Vinuesa MJ Alonso Protein-loaded PLGAndashPEO blend nanoparticlesencapsulation release and degradation characteristics Colloid Polym Sci288 (2010) 141ndash150 httpdxdoiorg101007s00396-009-2131-z
[13] N Pirooznia S Hasannia A Lotfi M Ghanei Encapsulation of alpha-1antitrypsin in PLGA nanoparticles in Vitro characterization as an effectiveaerosol formulation in pulmonary diseases J Nanobiotechnol 10 (2012) 20httpdxdoiorg1011861477-3155-10-20
[14] I DrsquoAngelo M Garcia-Fuentes Y Parajoacute A Welle T Vaacutentus A Horvaacuteth GBoumlkoumlnyi G Keacuteri MJ Alonso Nanoparticles based on PLGA poloxamer blendsfor the delivery of proangiogenic growth factors Mol Pharm 7 (2010)1724ndash1733 httpdxdoiorg101021mp1001262
[15] LJ White GTS Kirby HC Cox R Qodratnama O Qutachi FRAJ Rose KMShakesheff Accelerating protein release from microparticles for regenerativemedicine applications Mater Sci Eng C 23 (2013) 2578ndash2583 httpdxdoiorg101016jmsec201302020
[16] P Pantazis K Dimas JH Wyche S Anant CW Houchen J Panyam RPRamanujam Preparation of siRNA-Encapsulated PLGA nanoparticles forsustained release of siRNA and evaluation of encapsulation efficiencyNanopart Biol Med (2012) 311ndash319 httpdxdoiorg101007978-1-61779-953-2 25 Humana Press Totowa NJ
[17] JS Park HN Yang DG Woo SY Jeon KH Park Multilineage differentiationof human-derived dermal fibroblasts transfected with genes coated on PLGAnanoparticles plus growth factors Biomaterials 34 (2013) 582ndash597 httpdxdoiorg101016jbiomaterials201210001
[18] F Wan M Yang Design of PLGA-based depot delivery systems forbiopharmaceuticals prepared by spray drying Int J Pharm 498 (2016)82ndash95 httpdxdoiorg101016jijpharm201512025
[19] A Giteau MC Venier-Julienne A Aubert-Poueumlssel JP Benoit How to achievesustained and complete protein release from PLGA-based microparticles IntJ Pharm 350 (2008) 14ndash26 httpdxdoiorg101016jijpharm200711012
[20] S Fredenberg M Wahlgren M Reslow A Axelsson The mechanisms of drugrelease in poly(lactic-co-glycolic acid)-based drug delivery systemsmdashareview Int J Pharm 415 (2011) 34ndash52 httpdxdoiorg101016jijpharm201105049
[21] F Mohamed CF van der Walle Engineering biodegradable polyesterparticles with specific drug targeting and drug release properties J PharmSci 97 (2008) 71ndash87 httpdxdoiorg101002jps21082
[22] N Csaba L Gonzaacutelez A Saacutenchez MJ Alonso Design and characterisation ofnew nanoparticulate polymer blends for drug delivery J Biomater Sci PolymEd 15 (2004) 1137ndash1151 httpdxdoiorg1011631568562041753098
[23] HK Makadia SJ Siegel Poly lactic-co-glycolic acid (PLGA) as biodegradablecontrolled drug delivery carrier Polymers (Basel) 3 (2011) 1377ndash1397 httpdxdoiorg103390polym3031377
[24] F Danhier E Ansorena JM Silva R Coco A Le Breton V Preacuteat PLGA-basednanoparticles an overview of biomedical applications J Controlled Release161 (2012) 505ndash522 httpdxdoiorg101016jjconrel201201043
[25] G Ratzinger U Laumlnger L Neutsch F Pittner M Wirth F Gabor Surfacemodification of PLGA particles the interplay between stabilizer ligand sizeand hydrophobic interactions Langmuir 26 (2010) 1855ndash1859 httpdxdoiorg101021la902602z
[26] FT Meng GH Ma W Qiu ZG Su WOW double emulsion technique usingethyl acetate as organic solvent effects of its diffusion rate on thecharacteristics of microparticles J Controlled Release 91 (2003) 407ndash416httpdxdoiorg101016S0168-3659(03)00273-6
[27] M Padial-Molina F OrsquoValle A Lanis F Mesa DM Dohan Ehrenfest H-LWang P Galindo-Moreno Clinical application of mesenchymal stem cells andnovel supportive therapies for oral bone regeneration Biomed Res Int 2015(2015) httpdxdoiorg1011552015341327
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[28] P Yilgor N Hasirci V Hasirci Sequential BMP-2BMP-7 delivery frompolyester nanocapsules J Biomed Mater Res ndash Part A 93 (2010) 528ndash536httpdxdoiorg101002jbma32520
[29] B Li T Yoshii AE Hafeman JS Nyman JC Wenke SA Guelcher The effectsof rhBMP-2 released from biodegradable polyurethanemicrospherecomposite scaffolds on new bone formation in rat femora Biomaterials 30(2009) 6768ndash6779 httpdxdoiorg101016jbiomaterials200908038
[30] Y Wang Y Wei X Zhang M Xu F Liu Q Ma Q Cai X Deng PLGAPDLLAcore-shell submicron spheres sequential release system preparationcharacterization and promotion of bone regeneration in vitro and in vivoChem Eng J 273 (2015) 490ndash501 httpdxdoiorg101016jcej201503068
[31] YB Shim HH Jung JW Jang HS Yang H Bae JC Park B Choi SH LeeFabrication of hollow porous PLGA microspheres using sucrose for controlleddual delivery of dexamethasone and BMP2 J Ind Eng Chem 37 (2016)101ndash106 httpdxdoiorg101016jjiec201603014
[32] J Maldonado-Valderrama JAH Terriza A Torcello-Goacutemez MACabrerizo-Viacutelchez In vitro digestion of interfacial protein structures SoftMatter (2013) 1043ndash1053 httpdxdoiorg101039c2sm26843d
[33] M a Cabrerizo-Vilchez H a Wege J a Holgado-Terriza a W NeumannAxisymmetric drop shape analysis as penetration Langmuir balance Rev SciInstrum 70 (1999) 2438ndash2444 httpdxdoiorg10106311149773
[34] PA Hassan S Rana G Verma Making sense of brownian motion colloidcharacterization by dynamic light scattering Langmuir 31 (2015) 3ndash12httpdxdoiorg101021la501789z
[35] RJ Kok M Haas F Moolenaar D de Zeeuw DK Meijer Drug delivery to thekidneys and the bladder with the low molecular weight protein lysozymeRen Fail 20 (1998) 211ndash217
[36] S Mason SA Tarle W Osibin Y Kinfu D Kaigler Standardization and safetyof alveolar bone-derived stem cell isolation J Dent Res 93 (2014) 55ndash61httpdxdoiorg1011770022034513510530
[37] C Farace P Saacutenchez-Moreno M Orecchioni R Manetti F Sgarrella Y AsaraJM Peula-Garciacutea JA Marchal R Madeddu LG Delogu Immune cell impactof three differently coated lipid nanocapsules pluronic chitosan andpolyethylene glycol Sci Rep 6 (2016) 18423 httpdxdoiorg101038srep18423
[38] C Sturesson J Carlfors Incorporation of protein in PLG-microspheres withretention of bioactivity J Controlled Release 67 (2000) 171ndash178 httpdxdoiorg101016S0168-3659(00)00205-4
[39] L Ying S Jiali J Guoqiang Z Jia D Fuxin In vitro evaluation oflysozyme-loaded microspheres in thermosen- sitive methylcellulose-basedhydrogel Chin J Chem Eng 15 (2007) 566ndash572
[40] C Cai U Bakowsky E Rytting AK Schaper T Kissel Charged nanoparticlesas protein delivery systems a feasibility study using lysozyme as modelprotein Eur J Pharm Biopharm 69 (2008) 31ndash42 httpdxdoiorg101016jejpb200710005
[41] A Paillard-Giteau VT Tran O Thomas X Garric J Coudane S Marchal IChourpa JP Benoicirct CN Montero-Menei MC Venier-Julienne Effect ofvarious additives and polymers on lysozyme release from PLGA microspheresprepared by an sow emulsion technique Eur J Pharm Biopharm 75 (2010)128ndash136 httpdxdoiorg101016jejpb201003005
[42] MJ Santander-Ortega D Bastos-Gonzaacutelez JL Ortega-Vinuesa MJ AlonsoInsulin-loaded PLGA nanoparticles for oral administration an in vitrophysico-chemical characterization J Biomed Nanotechnol 5 (2009) 45ndash53httpdxdoiorg101166jbn2009022
[43] ID Rosca F Watari M Uo Microparticle formation and its mechanism insingle and double emulsion solvent evaporation J Controlled Release 99(2004) 271ndash280 httpdxdoiorg101016jjconrel200407007
[44] S Pezennec F Gauthier C Alonso F Graner T Croguennec G Bruleacute ARenault The protein net electric charge determines the surface rheologicalproperties of ovalbumin adsorbed at the air-water interface FoodHydrocolloids 14 (2000) 463ndash472 httpdxdoiorg101016S0268-005X(00)00026-6
[45] A Torcello-Goacutemez MJ Santander-Ortega JM Peula-Garciacutea JMaldonado-Valderrama MJ Gaacutelvez-Ruiz JL Ortega-Vinuesa AMartiacuten-Rodriacuteguez Adsorption of antibody onto pluronic F68-coverednanoparticles link with surface properties Soft Matter 7 (2011) 8450 httpdxdoiorg101039c1sm05570d
[46] MJ Santander-Ortega MV Lozano-Loacutepez D Bastos-Gonzaacutelez JMPeula-Garciacutea JL Ortega-Vinuesa Novel core-shell lipid-chitosan andlipid-poloxamer nanocapsules stability by hydration forces Colloid PolymSci 288 (2010) 159ndash172 httpdxdoiorg101007s00396-009-2132-y
[47] A Torcello-Goacutemez MJ Santander-Ortega JM Peula-Garciacutea JMaldonado-Valderrama MJ Gaacutelvez-Ruiz JL Ortega-Vinuesa AMartiacuten-Rodriacuteguez Adsorption of antibody onto Pluronic F68-coverednanoparticles link with surface properties Soft Matter 7 (2011) httpdxdoiorg101039c1sm05570d
[48] JP Penaloza V Maacuterquez-Miranda M Cabana-Brunod R Reyes-Ramiacuterez FMLlancalahuen C Vilos F Maldonado-Biermann LA Velaacutesquez JA FuentesFD Gonzaacutelez-Nilo M Rodriacuteguez-Diacuteaz C Otero Intracellular trafficking andcellular uptake mechanism of PHBV nanoparticles for targeted delivery inepithelial cell lines J Nanobiotechnol 15 (2017) 1 httpdxdoiorg101186s12951-016-0241-6
[49] SP Schwendeman RB Shah BA Bailey AS Schwendeman Injectablecontrolled release depots for large molecules J Controlled Release 190 (2014)240ndash253 httpdxdoiorg101016jjconrel201405057
[50] H Wang SCG Leeuwenburgh Y Li JA Jansen The use of micro- andnanospheres as functional components for bone tissue regeneration TissueEng Part B Rev 18 (2012) 24ndash39 httpdxdoiorg101089tenteb20110184
[51] JK Vasir V Labhasetwar Biodegradable nanoparticles for cytosolic deliveryof therapeutics Adv Drug Deliv Rev 59 (2007) 718ndash728 httpdxdoiorg101016jaddr200706003
[52] B Yameen W Il Choi C Vilos A Swami J Shi OC Farokhzad Insight intonanoparticle cellular uptake and intracellular targeting J Controlled Release190 (2014) 485ndash499 httpdxdoiorg101016jjconrel201406038
[53] H Sneh-Edri D Likhtenshtein D Stepensky Intracellular targeting of PLGAnanoparticles encapsulating antigenic peptide to the endoplasmic reticulumof dendritic cells and its effect on antigen cross-presentation in vitro MolPharm 8 (2011) 1266ndash1275 httpdxdoiorg101021mp200198c
[54] MJ Santander-Ortega AB Joacutedar-Reyes N Csaba D Bastos-Gonzaacutelez JLOrtega-Vinuesa Colloidal stability of Pluronic F68-coated PLGAnanoparticles a variety of stabilisation mechanisms J Colloid Interface Sci302 (2006) 522ndash529 httpdxdoiorg101016jjcis200607031
[55] B Baumann T Jungst S Stichler S Feineis O Wiltschka M Kuhlmann MLindn J Groll Control of nanoparticle release kinetics from 3D printedhydrogel scaffolds Angew Chem ndash Int Ed 56 (2017) 4623ndash4628 httpdxdoiorg101002anie201700153
[56] S-J Lee W Zhu L Heyburn M Nowicki B Harris LG Zhang Developmentof novel 3-D printed scaffolds with core-shell nanoparticles for nerveregeneration IEEE Trans Biomed Eng 64 (2017) 408ndash418 httpdxdoiorg101109tbme20162558493
[57] DJ Hines DL Kaplan Poly(lactic-co-glycolic) acid-controlled-releasesystems experimental and modeling insights Crit Rev Ther Drug CarrierSyst 30 (2013) 257ndash276 httpdxdoiorg101615CritRevTherDrugCarrierSyst2013006475
[58] H Begam SK Nandi B Kundu A Chanda Strategies for delivering bonemorphogenetic protein for bone healing Mater Sci Eng C 70 (2016)856ndash869 httpdxdoiorg101016jmsec201609074
[59] YH Kim Y Tabata Dual-controlled release system of drugs for boneregeneration Adv Drug Deliv Rev 94 (2015) 28ndash40 httpdxdoiorg101016jaddr201506003
[60] N Rescignano L Tarpani A Romani I Bicchi S Mattioli C Emiliani L TorreJM Kenny S Martino L Latterini I Armentano In-vitro degradation of PLGAnanoparticles in aqueous medium and in stem cell cultures by monitoring thecargo fluorescence spectrum Polym Degrad Stab 134 (2016) 296ndash304httpdxdoiorg101016jpolymdegradstab201610017
[61] M Morille T Van-Thanh X Garric J Cayon J Coudane D Noeumll MCVenier-Julienne CN Montero-Menei New PLGA-P188-PLGA matrix enhancesTGF-3 release from pharmacologically active microcarriers and promoteschondrogenesis of mesenchymal stem cells J Control Release 170 (2013)99ndash110 httpdxdoiorg101016jjconrel201304017
[62] A Bohr F Wan J Kristensen M Dyas E Stride S Baldursdottiacuter MEdirisinghe M Yang Pharmaceutical microparticle engineering withelectrospraying the role of mixed solvent systems in particle formation andcharacteristics J Mater Sci Mater Med 26 (2015) 61 httpdxdoiorg101007s10856-015-5379-5
[63] A Rafati A Boussahel KM Shakesheff AG Shard CJ Roberts X Chen DJScurr S Rigby-Singleton P Whiteside MR Alexander MC Davies Chemicaland spatial analysis of protein loaded PLGA microspheres for drug deliveryapplications J Control Release 162 (2012) 321ndash329 httpdxdoiorg101016jjconrel201205008
[64] R Gaudana M Gokulgandhi V Khurana D Kwatra AK Mitra Design andevaluation of a novel nanoparticulate-based formulation encapsulating a HIPcomplex of lysozyme Pharm Dev Technol 18 (2013) 752ndash759 httpdxdoiorg103109108374502012737806
[65] S Xiong X Zhao BC Heng KW Ng JSC Loo Cellular uptake of Poly-(DL-lactide-co-glycolide) (PLGA) nanoparticles synthesized through solventemulsion evaporation and nanoprecipitation method Biotechnol J 6 (2011)501ndash508 httpdxdoiorg101002biot201000351
[66] J Panyam V Labhasetwar Dynamics of endocytosis and exocytosis of poly (DL-lactide-co-glycolide) nanoparticles in vascular smooth muscle cells PharmRes 20 (2003) 212ndash220 httpwwwncbinlmnihgovpubmed12636159
pharmaceutics
Article
Formulation Colloidal Characterization and In VitroBiological Ecrarrect of BMP-2 Loaded PLGANanoparticles for Bone Regeneration
Teresa del Castillo-Santaella 1 Inmaculada Ortega-Oller 2 Miguel Padial-Molina 2 Francisco OrsquoValle 3 Pablo Galindo-Moreno 2 Ana Beleacuten Joacutedar-Reyes 14 andJoseacute Manuel Peula-Garciacutea 15
1 Biocolloid and Fluid Physics Group Department of Applied Physics University of Granada 18071 GranadaSpain
2 Department of Oral Surgery and Implant Dentistry University of Granada 18071 Granada Spain3 Department of Pathology School of Medicine amp IBIMER University of Granada 18071 Granada Spain4 Excellence Research Unit ldquoModeling Naturerdquo (MNat) University of Granada 18071 Granada Spain5 Department of Applied Physics II University of Malaga 29071 Malaga Spain Correspondence jmpeulaumaes Tel +34-952132722
Received 20 June 2019 Accepted 31 July 2019 Published 3 August 2019$amp($amp
Abstract Nanoparticles (NPs) based on the polymer poly (lactide-co-glycolide) acid (PLGA) havebeen widely studied in developing delivery systems for drugs and therapeutic biomolecules due tothe biocompatible and biodegradable properties of the PLGA In this work a synthesis method forbone morphogenetic protein (BMP-2)-loaded PLGA NPs was developed and optimized in order tocarry out and control the release of BMP-2 based on the double-emulsion (wateroilwater WOW)solvent evaporation technique The polymeric surfactant Pluronic F68 was used in the synthesisprocedure as it is known to have an ecrarrect on the reduction of the size of the NPs the enhancement oftheir stability and the protection of the encapsulated biomolecule Spherical solid polymeric NPswere synthesized showing a reproducible multimodal size distribution with diameters between100 and 500 nm This size range appears to allow the protein to act on the cell surface and at thecytoplasm level The ecrarrect of carrying BMP-2 co-adsorbed with bovine serum albumin on the NPsurface was analyzed The colloidal properties of these systems (morphology by SEM hydrodynamicsize electrophoretic mobility temporal stability protein encapsulation and short-term release profile)were studied The ecrarrect of both BMP2-loaded NPs on the proliferation migration and osteogenicdicrarrerentiation of mesenchymal stromal cells from human alveolar bone (ABSC) was also analyzedin vitro
Keywords BMP-2 PLGA nanoparticles Pluronic F68
1 Introduction
In the context of nanomedicine tissue regeneration using colloidal micro- and nano-structureshaving unique size and surface activity has received increasing attention over recent years Many ecrarrortshave been made to improve the engineering of these nano-systems in order to reach a ldquosmartrdquo deliveryof bioactive molecules in order to optimize their therapeutic advantages and minimize harmful sideecrarrects [1] With this aim a broad spectrum of biocompatible nanocarriers has been described showingproperties suitable for dicrarrerent biological and therapeutic applications [2] Among these variedproposals polymeric nanosystems represent a major group in which poly lactic-co-glycolic acid (PLGA)is one of the most widely used due to its biocompatibility biodegradability and low cytotoxicitygaining the approval from dicrarrerent drug agencies for human use [34]
Pharmaceutics 2019 11 388 doi103390pharmaceutics11080388 wwwmdpicomjournalpharmaceutics
Pharmaceutics 2019 11 388 2 of 18
PLGA-based structures are described as micro- and nanocarriers to deliver a wide variety of activemolecules and drugs synthetic or natural molecules with hydrophilic or hydrophobic properties andbiomolecules from proteins to nucleic acids [5ndash7] PLGA micro- and nanosystems can be set up usingdicrarrerent formulation techniques with the possibility of a systemic or local distribution These systemscan be applied not only in tissue regeneration but also in very diverse therapies Anticancer drugdelivery infections inflammatory diseases or gene therapy [3] Despite this great potential certainapplications especially in protein encapsulation are hindered by problems such as an uncontrolledrelease profile and protein denaturation [8ndash11]
The water-in oil-in water (WOW) double emulsion method is an ldquoemulsion solvent evaporationrdquotechnique frequently used to encapsulate hydrophilic molecules as proteins in PLGA NPs [612]The appropriate choice of organic solvents the use of polymer-surfactant blends and the addition ofstabilizer-protective agents have proved to be key aspects for optimizing the resulting systems [911]Additionally a surface specific functionalization can be used to improve their versatility allowingthe chemical surface immobilization of dicrarrerent molecules in order to confer targeting or adhesiveproperties to these nanocarriers [13]
Within tissue engineering bone regeneration has a broad range of applications mostly in the fieldof dentistry where PLGA is suggested as a reference polymer to formulate NPs with bone-healinguses [14] The literature describes the delivery of bioactive molecules normally growth factors usingpolymeric microparticles (MPs) and NPs with PLGA as the main component [13] Among the bonemorphogenetic growth factors BMP-2 (bone morphogenetic protein 2) has been the most frequentlycited with many examples in which encapsulation or surface adsorption enables adequate entrapmenteciency and diverse release patterns [15ndash19] For proteins with a very short half-life such as BMPsbiodegradable PLGA nanosystems provide protection and optimal dosage for an adequate stimulationof cell dicrarrerentiation [2021]
Thus within this scenario in the present work we seek to optimize a nano-particulate system inorder to carry out and control the release of BMP-2 using as a starting point the synthesis procedure ofa lysozyme-loaded NP system previously described for the encapsulation of that model protein [11]Also to encapsulate BMP-2 we prepared a second system in which this protein was co-adsorbedwith bovine serum albumin onto the surface of empty NPs The size and morphology the proteinencapsulation eciency the surface characteristics and the colloidal and temporal stability werestudied to complete the physico-chemical characterization of both NP systems
The release profile of BMP-2 indicates the potential of a PLGA nanocarrier for bone regenerationand depends heavily on the polymer degradation by hydrolysis [22] However over the shortterm during which the release does not depend on this chemical degradation proper control ofrelease is necessary in order to modulate other physical processes Thus we focused our releaseexperiments on the short-term using dicrarrerent techniques to compare the two NP samples and establishthe corresponding BMP-2 release profiles Finally the biological activity (cell migration proliferationand osteogenic dicrarrerentiation) was tested in vitro using mesenchymal stromal cells (MSCs) derivedfrom alveolar bone [23]
2 Materials and Methods
21 Nanoparticle Synthesis
211 Formulation
Poly(lactide-co-glycolide) acid (PLGA 5050) ([C2H2O2]x[C3H4O2]y) x = 50 y = 50 (Resomerreg
503H (Evonik Essen Germany) 32ndash44 kDa was used as the polymer and polymeric surfactantPluronic F68 (Poloxamer 188) (Sigma-Aldrich St Louis MO USA) as the emulsifier Their structurebased on a poly(ethylene oxide)-block-poly(propylene oxide)-block-poly(ethylene oxide) is expressedas PEOa-PPOb-PEOa with a = 75 and b = 30 Human recombinant bone morphogenetic proteinrhBMP-2 (Sigma-H4791) was used as therapeutic biomolecule Water was purified in a Milli-Q
Pharmaceutics 2019 11 388 3 of 18
Academic Millipore system A double-emulsion synthesis method was used following a procedurepreviously described with slight modifications [11] In this method 100 mg of PLGA and 3 mg ofdeoxycholic acid (DC) were dissolved in a tube containing 1 mL of ethyl acetate (EA) and vortexedIn total 40 microL of a bucrarrered solution at pH 128 with or without rhBMP-2 (200 microgmL) were addedand immediately sonicated (Branson Ultrasonics 450 Analog Sonifier) for 1 min (Duty cycle dial 20Output control dial 4) with the tube surrounded by ice This primary WO emulsion was poured intoa plastic tube containing 2 mL of a bucrarrered solution (pH 12) of F68 at 1 mgmL and vortexing for30 s Then the tube surrounded by ice was sonicated at the maximum amplitude for the micro tip for1 min (Output control 7) This second WOW emulsion was poured into a glass containing 10 mL ofthe bucrarrered F68 solution and kept under magnetic stirring for 2 min The organic solvent was thenrapidly extracted by evaporation under vacuum to a final volume of 8 mL The resulting empty andBMP-2 encapsulated NP systems were named NP and NP-BMP2 respectively A detailed scheme ofthe synthesis procedure with a yield based on the PLGA component always higher than 85 is shownin Figure S1 of the Supplementary Materials
212 Cleaning and Storage
After the organic solvent evaporation the sample was centrifuged for 10 min at 20 C at 12000 rpmThe supernatant was filtered using Millipore nanofilters 01microm for measuring the free non-encapsulatedprotein The pellet was then resuspended in phosphate bucrarrer (115 mM NaH2PO4) PB to a finalvolume of 4 mL and kept refrigerated at 4 C Under these conditions the systems kept colloidalstability at least for one month
213 Protein Loading and Encapsulation Eciency
The initial protein loading was optimized for the nanoparticle formulation preserving the finalcolloidal stability after the evaporation step and taking into account the amounts shown in the literaturefor this growth factor when encapsulated inside PLGA NPs [2425] Thus we chose 2 microg as the initialtotal mass of rhBMP-2 which means a relation of 2 105 ww (rhBMP-2PLGA) The amount ofencapsulated rhBMP-2 was calculated by measuring the dicrarrerence between the initial added amountand the free non-encapsulated protein present in the supernatant after the cleaning step which wastested by a specific enzyme-linked immuno-sorbent assay following the instructions of the manufacturer(ELISA kit RAB0028 from Sigma-Aldrich St Louis MO USA) Then protein-encapsulation eciency(EE) was calculated as follows
EE =MI MF
MI 100
where MI is the initial total mass of rhBMP-2 and MF is the total mass of rhBMP-2 in theaqueous supernatant
214 Physical Protein Adsorption
Bovine serum albumin (BSA) and rhBMP-2 were coupled on the empty nanoparticle surface bya physical adsorption method The appropriate volume of an aqueous protein solution containing05 mg of BSA and 2 microg of rhBMP-2 was mixed with 5 mL of acetate bucrarrer (pH 5) containing emptyNPs with 125 mg of PLGA This provided a starting amount of proteins corresponding to 004 ww(proteinPLGA) while the mass relation between proteins was 04 ww (rhBMP-2BSA) This solutionwas incubated at room temperature for 2 h under mechanical stirring The nanoparticles were separatedfrom the bucrarrer solution by centrifugation and after the supernatants were filtered (Millipore nanofilters01 microm) they were qualitatively analyzed by gel electrophoresis while the protein quantification wasmade by a bicinchoninic acid protein assay (BCA) (Sigma-Aldrich St Louis MO USA) for BSA andthe specific ELISA for rhBMP-2 The nanoparticle pellet was resuspended in phosphate bucrarrer (pH 74)and stored at 4 C This system was named NP-BSA-BMP2
Pharmaceutics 2019 11 388 4 of 18
215 Protein Separation by Gel Electrophoresis SDS-PAGE
The protein-loaded NPs and dicrarrerent supernatants were treated at 90 C for 10 min in thefollowing bucrarrer 625 mM Tris-HCl (pH 68 at 25 C) 2 (wv) sodium dodecyl sulfate (SDS) 10glycerol 001 (wv) bromophenol blue 40 mM dithiothreitol (DTT) Samples were then separated bysize in porous 12 polyacrylamide gel (1D SDS polyacrylamide gel electrophoresis) under the ecrarrectof an electric field The electrophoresis was run under constant voltage (130 V 45 min) and the gelswere stained using a Coomassie Blue solution (01 Coomassie Brilliant Blue R-250 50 methanol and10 glacial acetic acid) and destained with the same solution lacking the dye
22 Nanoparticle Characterization Morphology Size Concentration and Electrokinetic Mobility
NPs were imaged by scanning electron microscopy (SEM) with a Zeiss SUPRA 40VP field-emissionscanning electron microscope from the Scientific Instrumentation Center of the University of Granada(CIC UGR)
The hydrodynamic size distribution of the NPs was evaluated by nanoparticle tracking analysis(NTA) with a NanoSight LM10-HS (GB) FT14 (NanoSight Amesbury UK) and an sCMOS cameraThe particle concentration according to the diameter (size distribution) was calculated as an averageof at least three independent size distributions The total concentration of NPs of each system wasdetermined in order to control the number of particles used in cell experiments The measurementconditions for all samples were 25 C a viscosity of 089 cP a measurement time of 60 s and a cameragain of 250 The camera shutter was 11 and 15 ms for the empty and BMP-loaded NPs respectivelyThe detection threshold was fixed at 5
The electrophoretic mobility of the NPs was determined using a Zetasizerreg NanoZeta ZS device(Malvern Instrument Ltd Malvern UK) working at 25 C with an He-Ne laser of 633 nm and a 173
scattering angle Each data point was taken as an average over three independent sample measurementsFor each sample the electrophoretic mobility distribution and the average electrophoretic mobility(micro-average) were determined by the technique of laser Doppler electrophoresis
23 Colloidal and Temporal Stability in Biological Media
The average hydrodynamic diameter and the polydispersity index (PDI) by dynamic lightscattering (DLS) of each NP system were measured in dicrarrerent media (phosphate bucrarrer (PB) salinephosphate bucrarrer (PBS) and cell culture medium Dulbeccorsquos modified Eaglersquos medium DMEM(Sigma)) Also data on temporal stability were gathered by repeating these analyses at dicrarrerent timesafter synthesis (0 1 and 5 days) and after 1 month under storage conditions
In vitro release experiments were conducted as follows 1 mL of each sample for each incubationtime was suspended in PBS at 37 C After the corresponding time (24 48 96 168 h) NPs wereseparated from the supernatant of released proteins by centrifugation for 10 min at 14000 rpm (10 C)The NP pellet was suspended in 1 mL of 005 M NaOH and stirred for 2 h for a complete polymerdegradation The alkaline protein solution was assayed by BCA and ELISA to quantify the unreleasedamount The protein released was calculated taking into account the total encapsulated amount Allexperiments were made in triplicate
24 Cell Interactions
For all biological in vitro studies a cell population cultured from the maxillary alveolar bonewas used This population was previously characterized and confirmed to present all characteristicsof a mesenchymal stromal cell population (MSC) [23] Cells were taken from healthy humandonors after the approval from the Ethics Committee for Human Research from the University ofGranada (424CEIH2018) Regular Dulbeccorsquos modified Eaglersquos medium (DMEM) with 1 gL glucose(DMEM-LG) (Gibco) 10 fetal bovine serum (FBS) (Sigma-Aldrich St Louis MO USA) 1100 ofnon-essential amino acid solution (NEAA) (Gibco) 001 microgmL of basic fibroblast growth factor (bFGF)
Pharmaceutics 2019 11 388 5 of 18
(PeproTech London UK) 100 UmL of penicillinstreptomycin and 025 microgmL of amphotericin Bwas used as culture medium for all experiments Cultures were maintained at 37 C in a 5 CO2atmosphere (2000 cellswell) All biological experiments were repeated in triplicate at least 3 timesper condition
241 Cell Migration
A cell-migration assay was conducted as previously described [2627] Briefly MSCs weredistributed on to three wells for each condition and allowed to grow to a cell confluency close to99 in 24-wellsplate at 3000 cellscm2 and in each well three dicrarrerent scratches were made Thencells were starved for 24 h by adding culture medium without serum A scratch was made usinga pipette tip along the diameter of the well A wash step with PBS was performed to remove thescratched cells Fresh complete culture media was added and supplemented depending on the assignedgroup (BMP-2 NP- BMP2 and NP-BSA-BMP2 at 125 25 and 5 ngmL of BMP-2) Afterwardsnine images were taken from the same area in each condition until 48 h later On these images thescraped area was measured by ImageJ software (National Institute of Health Bethesda MD USAhttprsbwebnihgovij) The reduction in the scratched area over time was measured consideringthe area at time 0 as 100 open
242 Cell Proliferation
Proliferation was evaluated by a sulphorhodamine (SRB) assay [28] The assay was conducted byseeding the cells at 1500 cellscm2 in a 96-well plate at a confluence not higher than 50 After cellattachment the dicrarrerent supplements were added (BMP-2 NP- BMP2 and NP-BSA-BMP2 at 12525 and 5 ngmL of BMP-2) and the cells were maintained in culture for up to 7 days At each timepoint the cells were washed with 1X PBS and fixed by adding ice-cold 10 trichloroacetic acid for20 min at 4 C Then the cells were washed 3 times with dH2O and dried until all time points werecollected Each well received 04 SRB in 1 acetic acid for 20 min at room temperature with gentleshaking The staining was finished by washing each well 3 times with 1 acetic acid and drying it atroom temperature for 24 h The dye was retrieved from the cells by adding 10 mM Tris Base at pH 105and gently shaking for 10 min The solution recovered was then distributed in a 96-well plate and theoptical absorbance was read at 492 nm
243 Osteogenic Dicrarrerentiation
Osteogenic dicrarrerentiation was evaluated by adding osteogenic media to the cell culture incombination with free BMP-2 NP-BMP2 and NP-BSA-BMP2 at the highest dosages used inprevious experiments Cells were seeded at 3000 cellscm2 and cultured to reach an 85 to90 confluency This was followed by the addition of induction media containing 10 mM of-glycerophosphate (Fluka 50020) 01 microM of dexamethasone (Sigma-Aldrich D2915) and 005 mMof L-ascorbic acid (Sigma-Aldrich A8960) Cell cultures were maintained for 7 days to analyzeearly activity At day 7 cells were collected in 1 mL of TRIzolreg Then RNA was extracted andconverted to cDNA Alkaline phosphatase (ALP) was then evaluated expression being calculatedrelative to glyceraldehyde-3-phosphate dehydrogenase protein (GAPDH) by the 2DDCt methodThese procedures were conducted as described elsewhere [23] Forward and reverse primer sequenceswere AGCTCATTTCCTGGTATGACAAC and TTACTCCTTGGAGGCCATGTG for GAPDH andTCCAGGGATAAAGCAGGTCTTG and CTTTCTCTTTCTCTGGCACTAAGG for ALP
244 Statistical Evaluation
Cell migration and proliferation were evaluated by ANOVA followed by Tukey multiplecomparisons test for pairwise analysis Comparison between the levels of ALP at 4 vs 7 dayswere analyzed by paired Studentrsquos t test In all cases a p value lower than 005 was established asstatistical significance
Pharmaceutics 2019 11 388 6 of 18
3 Results and Discussion
31 Nanoparticle Formulation
Double emulsion-solvent evaporation has been described as a robust and frequently used methodto produce biomolecule-loaded PLGA NPs [6121329] A formulation previously optimized byour group enabled the preservation of the biological activity of encapsulated biomolecules using aslightly aggressive organic solvent Moreover deoxycholic acid has been used in the first step of theformulation in order to improve the colloidal stability of NPs and simultaneously to obtain NP surfacesenriched with carboxylic groups improving their versatility and allowing a subsequent chemicalimmobilization of dicrarrerent specific ligands [30] By means of this improved formulation in the presentwork we developed empty nanoparticles (NPs) or nanoparticles encapsulating rhBMP-2 (NP-BMP2)A schematic description of the synthesis procedure is shown in Figure S1 of the Supplementary DataFor NP-BMP2 we achieved a protein-encapsulation eciency (EE) of 97 plusmn 2 This result is consistentwith the literature in which several authors have reported similarly high values encapsulating thisprotein inside PLGA nano- and microparticles [3132] Our formulation has several factors leading tothis very high EE value The low proteinpolymer relation in mass [33] the anity of rhBMP-2 to anunspecific interaction with hydrophobic surfaces [31] or the addition of stabilizers (poloxamer) inthe second step of the double-emulsion procedure [13] The absence of rhBMP-2 in the supernatantresulting from the centrifugation step in the cleaning process was verified by ELISA and SDS-PAGE inwhich a clear band corresponding to 14 kD of rhBMP-2 polypeptidic chains is shown for lane A inFigure 1 corresponding to NP-BMP2 The mass of protein encapsulated around 2 microg is similar to thatof dicrarrerent PLGA micro- and nanosystems described in the literature [183435] Taking into accountthe storage conditions for our samples this corresponds to 500 ngmL which represents a sucientconcentration for practical applications since this growth factor shows in vitro biological activities atvery low dosages (5ndash20 ngmL) [13]
Pharmaceutics 2019 11 x 6 of 18
31 Nanoparticle Formulation
Double emulsion-solvent evaporation has been described as a robust and frequently used method to produce biomolecule-loaded PLGA NPs [6121329] A formulation previously optimized by our group enabled the preservation of the biological activity of encapsulated biomolecules using a slightly aggressive organic solvent Moreover deoxycholic acid has been used in the first step of the formulation in order to improve the colloidal stability of NPs and simultaneously to obtain NP surfaces enriched with carboxylic groups improving their versatility and allowing a subsequent chemical immobilization of different specific ligands [30] By means of this improved formulation in the present work we developed empty nanoparticles (NPs) or nanoparticles encapsulating rhBMP-2 (NP-BMP2) A schematic description of the synthesis procedure is shown in Figure S1 of the Supplementary Data For NP-BMP2 we achieved a protein-encapsulation efficiency (EE) of 97 plusmn 2 This result is consistent with the literature in which several authors have reported similarly high values encapsulating this protein inside PLGA nano- and microparticles [3132] Our formulation has several factors leading to this very high EE value The low proteinpolymer relation in mass [33] the affinity of rhBMP-2 to an unspecific interaction with hydrophobic surfaces [31] or the addition of stabilizers (poloxamer) in the second step of the double-emulsion procedure [13] The absence of rhBMP-2 in the supernatant resulting from the centrifugation step in the cleaning process was verified by ELISA and SDS-PAGE in which a clear band corresponding to 14 kD of rhBMP-2 polypeptidic chains is shown for lane A in Figure 1 corresponding to NP-BMP2 The mass of protein encapsulated around 2 microg is similar to that of different PLGA micro- and nanosystems described in the literature [183435] Taking into account the storage conditions for our samples this corresponds to 500 ngmL which represents a sufficient concentration for practical applications since this growth factor shows in vitro biological activities at very low dosages (5ndash20 ngmL) [13]
Figure 1 SDS polyacrylamide gel electrophoresis (SDS-PAGE) analysis under reducing conditions of solid PLGA Nanoparticles (PLGA NPs) and liquid (supernatant) fractions of different NP systems Lane P Protein standards lane A NP-BMP2 (bone morphogenetic protein) lane B supernatant of NP-BMP2 after synthesis and encapsulation of rhBMP-2 lane C NP after physical adsorption of BSArhBMP-2 lane D supernatant after physical adsorption of BSA(bovine serum albumin)rhBMP-2 on NP system
On the other hand a second nanosystem resulted modifying the way in which rhBMP-2 is incorporated in the nanocarrier There are several examples of surface adsorption of different growth factors in micro- and nanoparticles [35ndash37] and surface immobilization over the encapsulation has recently been proposed as a way to modulate the later release of biomolecules This process which
Figure 1 SDS polyacrylamide gel electrophoresis (SDS-PAGE) analysis under reducing conditions ofsolid PLGA Nanoparticles (PLGA NPs) and liquid (supernatant) fractions of dicrarrerent NP systems LaneP Protein standards lane A NP-BMP2 (bone morphogenetic protein) lane B supernatant of NP-BMP2after synthesis and encapsulation of rhBMP-2 lane C NP after physical adsorption of BSArhBMP-2lane D supernatant after physical adsorption of BSA(bovine serum albumin)rhBMP-2 on NP system
On the other hand a second nanosystem resulted modifying the way in which rhBMP-2 isincorporated in the nanocarrier There are several examples of surface adsorption of dicrarrerent growthfactors in micro- and nanoparticles [35ndash37] and surface immobilization over the encapsulation has
Pharmaceutics 2019 11 388 7 of 18
recently been proposed as a way to modulate the later release of biomolecules This process whichdepends on the slow dicrarrusion of biomolecules through the polymeric matrix is consequently highlyinfluenced by the proteinndashpolymer interaction [3839] and polymer degradation [36] Thus this newfocus on the use of PLGA NPs for biomolecule delivery was explored by immobilizing the proteinrhBMP-2 on the surface of empty NPs by means of simple physical adsorption This process is knownto be governed by electrostatic and hydrophobic interactions between protein molecules and NPsurfaces [40]
For this the surface-charged groups the hydrophilicity the net charge of the protein moleculesand the characteristics of the adsorption medium are the reference parameters Thus we designed aco-adsorption experiment in which a mixture of rhBMP-2 and BSA (04 ww rhBMP-2BSA) interactsimultaneously with the PLGA NP surface Albumins are routinely used as protective proteins whengrowth factors are incorporated in PLGA NPs [1319] Moreover a surface distribution of BSA moleculescan improve the colloidal stability of NPs at physiological pH due to their net negative charge underthese conditions [41] Figure S2 from Supplementary Materials shows a scheme of the co-adsorptionprocess The adsorption eciency is higher than 95 and in SDS-PAGE from Figure 1 two bandscharacteristic of both proteins can be seen in lane C corresponding to the NP-BSA-BMP2 nanosystemHowever lane D corresponding to the run of the supernatant from the centrifugation of the nanosystemafter adsorption processes shows the absence of any protein This result is fully explained by takinginto account the pH of the medium (pH 50) near the isoelectric point of BSA where the adsorption ofthis protein onto negatively charged nanoparticles presents a maximum [4042] The immobilizationof rhBMP-2 on the negatively charged surface of NPs proves they are electrostatically favored due tothe positive net charge of this protein at acid and neutral pH
32 Nanoparticle Characterization
321 Nanoparticle Size
SEM and STEM micrographs (Figure 2) show that the samples consist of spherical particles ofdicrarrerent diameters (between 150 and 450 nm) a range similar to that found in a previous work inwhich NPs were loaded with lysozyme following a similar synthesis protocol [11] In that workthe DLS technique failed to provide a reliable size distribution Therefore the NTA technique wasdirectly used to determine the hydrodynamic size of the BMP2-loaded NPs (see NTA video in theSupplementary Material)
The size distributions for empty (NP) and BMP-loaded NPs (NP-BMP2) from NTA (Figure 3 andvideos S1 S2) were consistent with the SEM images Particles with diameters between 100 and 500 nmwere found to have the highest particle concentration at around 200 nm The loading with BMP hadan ecrarrect on the size distribution leading to more defined peaks These measurements enabled usto determine the concentration of particles in the measured sample 688 plusmn 009 108 ppmL and519 plusmn 012 108 ppmL for NP and NP-BMP2 nanosystems respectively These values were used(by taking into account the corresponding dilution) to control the number of particles added in thecell experiments
Pharmaceutics 2019 11 388 8 of 18
Pharmaceutics 2019 11 x 7 of 18
depends on the slow diffusion of biomolecules through the polymeric matrix is consequently highly influenced by the proteinndashpolymer interaction [3839] and polymer degradation [36] Thus this new focus on the use of PLGA NPs for biomolecule delivery was explored by immobilizing the protein rhBMP-2 on the surface of empty NPs by means of simple physical adsorption This process is known to be governed by electrostatic and hydrophobic interactions between protein molecules and NP surfaces [40]
For this the surface-charged groups the hydrophilicity the net charge of the protein molecules and the characteristics of the adsorption medium are the reference parameters Thus we designed a co-adsorption experiment in which a mixture of rhBMP-2 and BSA (04 ww rhBMP-2BSA) interact simultaneously with the PLGA NP surface Albumins are routinely used as protective proteins when growth factors are incorporated in PLGA NPs [1319] Moreover a surface distribution of BSA molecules can improve the colloidal stability of NPs at physiological pH due to their net negative charge under these conditions [41] Figure S2 from Supplementary Materials shows a scheme of the co-adsorption process The adsorption efficiency is higher than 95 and in SDS-PAGE from Figure 1 two bands characteristic of both proteins can be seen in lane C corresponding to the NP-BSA-BMP2 nanosystem However lane D corresponding to the run of the supernatant from the centrifugation of the nanosystem after adsorption processes shows the absence of any protein This result is fully explained by taking into account the pH of the medium (pH 50) near the isoelectric point of BSA where the adsorption of this protein onto negatively charged nanoparticles presents a maximum [4042] The immobilization of rhBMP-2 on the negatively charged surface of NPs proves they are electrostatically favored due to the positive net charge of this protein at acid and neutral pH
32 Nanoparticle Characterization
321 Nanoparticle Size
SEM and STEM micrographs (Figure 2) show that the samples consist of spherical particles of different diameters (between 150 and 450 nm) a range similar to that found in a previous work in which NPs were loaded with lysozyme following a similar synthesis protocol [11] In that work the DLS technique failed to provide a reliable size distribution Therefore the NTA technique was directly used to determine the hydrodynamic size of the BMP2-loaded NPs (see NTA video in the Supplementary Material)
Figure 2 Scanning electron microscopy (SEM) micrograph of rhBMP-2-loaded nanoparticles (NP-BMP2)
Figure 2 Scanning electron microscopy (SEM) micrograph of rhBMP-2-loaded nanoparticles(NP-BMP2)
Pharmaceutics 2019 11 x 8 of 18
The size distributions for empty (NP) and BMP-loaded NPs (NP-BMP2) from NTA (Figure 3 and videos S1 S2) were consistent with the SEM images Particles with diameters between 100 and 500 nm were found to have the highest particle concentration at around 200 nm The loading with BMP had an effect on the size distribution leading to more defined peaks These measurements enabled us to determine the concentration of particles in the measured sample 688 plusmn 009 times 108 ppmL and 519 plusmn 012 times 108 ppmL for NP and NP-BMP2 nanosystems respectively These values were used (by taking into account the corresponding dilution) to control the number of particles added in the cell experiments
Figure 3 Hydrodynamic diameter distribution of NP (circles) and NP-BMP2 (thick black line) measured at pH 70 (phosphate buffer) by nanoparticle tracking analysis (NTA)
322 Electrokinetic Mobility and Colloidal Stability
The surface charge of nanoparticles can be analyzed using an electrokinetic study by measuring the electrophoretic mobility (microe) under different conditions Figure 4 shows the microe and zeta potential values for the three nanosystems NP NP-BMP2 and NP-BSA-BMP2 at low ionic strength and different pH values The electric surface charge of NPs resides in the carboxylic groups of the uncapped PLGA and deoxycholic acid molecules These functionalized groups are additionally useful due to the possibility of a chemical surface vectorization in order to develop directed delivery nanocarriers [43] It was previously confirmed that protonation of these acidic surface groups at pH values under their pKa value was tightly correlated with a loss of surface charge and consequently a reduction (in absolute value) of the electrophoretic mobility of the colloidal system [4445] Usually when colloidal particles are coated by protein molecules the microe values change markedly compared with the same bare surfaces and are influenced by the electrical charge of the adsorbed protein molecules [4647] The electrokinetic behavior of the NP-BMP2 system remains similar to that of NP and encapsulation of rhBMP-2 does not affect the surface charge distribution A similar result was reported by drsquoAngelo et al on encapsulating different growth factors in PLGA-poloxamer blend nanoparticles in the same proportion ww of proteinpolymer [24] This may be due to the low amount of encapsulated protein and its distribution in the inner part of the NPs (far from the surface) In our system this internal distribution may be favored by the encapsulating conditions where the basic pH (pH 120) of the water phase containing rhBMP-2 allows a negative charge of these protein molecules thereby preventing their electrostatic specific interaction with acidic groups of the NPs
0 200 400 600 80000
05
10
15
20
25
30
35
40
parti
cle
conc
entra
tion
(106 p
pm
L)
D(nm)
Figure 3 Hydrodynamic diameter distribution of NP (circles) and NP-BMP2 (thick black line) measuredat pH 70 (phosphate bucrarrer) by nanoparticle tracking analysis (NTA)
322 Electrokinetic Mobility and Colloidal Stability
The surface charge of nanoparticles can be analyzed using an electrokinetic study by measuringthe electrophoretic mobility (microe) under dicrarrerent conditions Figure 4 shows the microe and zeta potentialvalues for the three nanosystems NP NP-BMP2 and NP-BSA-BMP2 at low ionic strength anddicrarrerent pH values The electric surface charge of NPs resides in the carboxylic groups of the uncappedPLGA and deoxycholic acid molecules These functionalized groups are additionally useful due to thepossibility of a chemical surface vectorization in order to develop directed delivery nanocarriers [43]It was previously confirmed that protonation of these acidic surface groups at pH values undertheir pKa value was tightly correlated with a loss of surface charge and consequently a reduction (in
Pharmaceutics 2019 11 388 9 of 18
absolute value) of the electrophoretic mobility of the colloidal system [4445] Usually when colloidalparticles are coated by protein molecules the microe values change markedly compared with the samebare surfaces and are influenced by the electrical charge of the adsorbed protein molecules [4647]The electrokinetic behavior of the NP-BMP2 system remains similar to that of NP and encapsulationof rhBMP-2 does not acrarrect the surface charge distribution A similar result was reported by drsquoAngeloet al on encapsulating dicrarrerent growth factors in PLGA-poloxamer blend nanoparticles in the sameproportion ww of proteinpolymer [24] This may be due to the low amount of encapsulated proteinand its distribution in the inner part of the NPs (far from the surface) In our system this internaldistribution may be favored by the encapsulating conditions where the basic pH (pH 120) of the waterphase containing rhBMP-2 allows a negative charge of these protein molecules thereby preventingtheir electrostatic specific interaction with acidic groups of the NPs
Pharmaceutics 2019 11 x 9 of 18
Figure 4 Electrophoretic mobility and zeta potential vs pH in buffered media of low salinity (ionic strength equal to 0002 M) for the different nanosystems (black square) NP (blue triangle) NP-BMP2 (red circle) NP-BSA-BMP2
The electrokinetic distribution for the NP-BSA-BMP2 system radically changes As previously shown the very high adsorption efficiency leads to NPs with both proteins adsorbed around their surface This situation is closely correlated with the microe values from Figure 4 Taking into account the ww relation between adsorbed proteins (250 times higher for BSA) albumin molecules modulate the behavior at pH values below their isoelectric point (pI 47) where the positive net charge of BSA masks the original surface charge of NPs and even changes their original values to positive ones This is a typical result found for this protein-covering colloidal particles [4248] At neutral and basic pH values BSA molecules have a negative net charge and the slight decrease in the absolute microe values could be due to the reduction of the negative net surface charge of NPs which may be shielded at least in a small part by the positive charge of rhBMP-2 molecules under their basic isoelectric point (pI 90)
The colloidal stability for the different nanosystems (NP NP-BMP2 and NP-BSA-BMP2) was determined by analyzing the size distributions in various media (PB PBS and DMEM) at different times after synthesis (0 1 and 5 days) Size distributions similar to the original ones were found for the two formulations NP and NP-BMP2 in all the media analyzed This result was similar to that previously found for these types of NPs encapsulating lysozyme [11] in which the combination of electrostatic and steric interactions generated by surface chemical groups of NPs confer the stability mechanism that prevents colloidal aggregation [33] The decrease of the absolute value of the zeta potential for the NP-BSA-BMP2 system as a consequence of surface protein distribution does not affect its colloidal stability This system also maintains the same size distribution in the different media It is commonly accepted that a zeta potential higher than +30 or minus30 mV will give rise to a stable colloidal system [49] and the zeta potential value for NP-BSA-BMP2 is above minus30 mV Colloidal stability in PBS and DMEM typically used media for the development of scaffold or cell interactions
Figure 4 Electrophoretic mobility and zeta potential vs pH in bucrarrered media of low salinity (ionicstrength equal to 0002 M) for the dicrarrerent nanosystems (black square) NP (blue triangle) NP-BMP2(red circle) NP-BSA-BMP2
The electrokinetic distribution for the NP-BSA-BMP2 system radically changes As previouslyshown the very high adsorption eciency leads to NPs with both proteins adsorbed around theirsurface This situation is closely correlated with the microe values from Figure 4 Taking into account theww relation between adsorbed proteins (250 times higher for BSA) albumin molecules modulate thebehavior at pH values below their isoelectric point (pI 47) where the positive net charge of BSA masksthe original surface charge of NPs and even changes their original values to positive ones This is atypical result found for this protein-covering colloidal particles [4248] At neutral and basic pH valuesBSA molecules have a negative net charge and the slight decrease in the absolute microe values could bedue to the reduction of the negative net surface charge of NPs which may be shielded at least in asmall part by the positive charge of rhBMP-2 molecules under their basic isoelectric point (pI 90)
The colloidal stability for the dicrarrerent nanosystems (NP NP-BMP2 and NP-BSA-BMP2) wasdetermined by analyzing the size distributions in various media (PB PBS and DMEM) at dicrarrerenttimes after synthesis (0 1 and 5 days) Size distributions similar to the original ones were found for
Pharmaceutics 2019 11 388 10 of 18
the two formulations NP and NP-BMP2 in all the media analyzed This result was similar to thatpreviously found for these types of NPs encapsulating lysozyme [11] in which the combination ofelectrostatic and steric interactions generated by surface chemical groups of NPs confer the stabilitymechanism that prevents colloidal aggregation [33] The decrease of the absolute value of the zetapotential for the NP-BSA-BMP2 system as a consequence of surface protein distribution does not acrarrectits colloidal stability This system also maintains the same size distribution in the dicrarrerent media It iscommonly accepted that a zeta potential higher than +30 or 30 mV will give rise to a stable colloidalsystem [49] and the zeta potential value for NP-BSA-BMP2 is above 30 mV Colloidal stability in PBSand DMEM typically used media for the development of scacrarrold or cell interactions respectivelyassures the potential use of these nanosystems for in vitro or in vivo living environments Additionallythese systems maintained their size under storage in PB at 4 C for at least 1 month (data not shown)showing this to be an adequate medium for sample storage
323 Protein Release
One of the main problems for micro- or nanosystems of PLGA drug delivery is to find theappropriate release pattern for encapsulatedattached protein molecules A wide spectrum offormulations modulates this property by the use of dicrarrerent types of synthesis processes PLGApolymers co-polymers and stabilizers [313] An adequate limitation and control in the burst releaseis critical for BMPs in order to ensure long-term continuous release that favored by the polymerdegradation provides better in vivo action in driving bone and cartilage regeneration [20] Thereforewe previously developed a dual PLGA nanosystem for controlled short-term release where proteindicrarrusion and proteinndashpolymer interaction are the main factors governing this process [11]
In the present work NP-BMP2 and NP-BSA-BMP2 nanosystems represent two dicrarrerent ways inwhich rhBMP-2 was incorporated into the nanocarrier Figure 5A shows the cumulative release of bothproteins rhBMP-2 and BSA for dicrarrerent systems as a function of time in a short-term period (7 days)The encapsulated rhBMP-2 protein reaches an amount released of around 30 of the initial encapsulatedone while adsorbed rhBMP-2 despite its surface distribution is three times lower However BSAshows released amounts up to 80 of the initial adsorbed ones In all cases error bars correspond tothe standard deviations from three independent experiments Under these conditions the growthfactor encapsulated in NP-BMP2 presents a release pattern similar to that previously found with thesame formulation but using lysozyme as the protein [11] Poloxamer in the water phase of the synthesisprocess can be key in modulating both specific and unspecific interfacial protein interactions [50]Thus the relation between proteinndashpolymer interaction and protein dicrarrusion appears to be wellbalanced preventing an excessive initial burst and simultaneously maintaining the needed proteinflux to release around a third of the encapsulated rhBMP-2 in 7 days Although an excessive initialburst has been widely reported for PLGA NPs related with protein molecules close to the surface [6]this situation did not appear for the NP-BMP2 system this being consistent with the electrokineticbehavior that did not show the presence of protein near surface The literature ocrarrers some exampleswith reduced short-term release of BMP-2 using more hydrophilic PLGA-PEG co-polymers [16] or adicrarrerent synthesis process [25]
The release performance for the NP-BSA-BMP2 system also shown in Figure 5A presents notabledicrarrerences The electrokinetic profile has previously justified the surface location of BSA and rhBMP-2on the surface which could lead to a fast release of both proteins However results from Figure 5ABshow this trend only for the BSA protein that is released from NPs with about 20 of the initial amountremaining after seven days However up to 90 of the initial load of rhBMP-2 protein unlike BSAremains attached to the surface The NP surface with hydrophilic groups form poloxamer moleculesand a negative charge due to the abundant presence of carboxylic groups (end-groups of PLGA anddeoxycholic acid molecules) favor a desorption process for BSA whose molecules have a negativecharge under release conditions (physiological pH) This agrees with the results of other authors whoeven after encapsulating BSA in PLGA-poloxamer blend NPs achieved a fast burst release of above
Pharmaceutics 2019 11 388 11 of 18
40 to 50 of the initial protein amount [33] Moreover the co-encapsulation of albumins with growthfactors could strongly acrarrect its release profile causing an initial burst [2124] Otherwise the specificelectrostatic attraction between positive rhBMP-2 molecules and negative surface groups slows downthe short time release of this protein This result is in agreement with the low release of adsorbedBMP previously found using PLGA micro- and nanoparticles with uncapped acid end groups [3851]Thus the combination of dicrarrerent methods for trapping BMP-2 into and around NPs shows up thepossibility of attaining a properly controlled release balancing the interactions between polymersstabilizers and protein
Pharmaceutics 2019 11 x 10 of 18
respectively assures the potential use of these nanosystems for in vitro or in vivo living
environments Additionally these systems maintained their size under storage in PB at 4 degC for at
least 1 month (data not shown) showing this to be an adequate medium for sample storage
323 Protein Release
One of the main problems for micro- or nanosystems of PLGA drug delivery is to find the
appropriate release pattern for encapsulatedattached protein molecules A wide spectrum of
formulations modulates this property by the use of different types of synthesis processes PLGA
polymers co-polymers and stabilizers [313] An adequate limitation and control in the burst release
is critical for BMPs in order to ensure long-term continuous release that favored by the polymer
degradation provides better in vivo action in driving bone and cartilage regeneration [20] Therefore
we previously developed a dual PLGA nanosystem for controlled short-term release where protein
diffusion and proteinndashpolymer interaction are the main factors governing this process [11]
In the present work NP-BMP2 and NP-BSA-BMP2 nanosystems represent two different ways
in which rhBMP-2 was incorporated into the nanocarrier Figure 5A shows the cumulative release of
both proteins rhBMP-2 and BSA for different systems as a function of time in a short-term period (7
days) The encapsulated rhBMP-2 protein reaches an amount released of around 30 of the initial
encapsulated one while adsorbed rhBMP-2 despite its surface distribution is three times lower
However BSA shows released amounts up to 80 of the initial adsorbed ones In all cases error bars
correspond to the standard deviations from three independent experiments Under these conditions
the growth factor encapsulated in NP-BMP2 presents a release pattern similar to that previously
found with the same formulation but using lysozyme as the protein [11] Poloxamer in the water
phase of the synthesis process can be key in modulating both specific and unspecific interfacial
protein interactions [50] Thus the relation between proteinndashpolymer interaction and protein
diffusion appears to be well balanced preventing an excessive initial burst and simultaneously
maintaining the needed protein flux to release around a third of the encapsulated rhBMP-2 in 7 days
Although an excessive initial burst has been widely reported for PLGA NPs related with protein
molecules close to the surface [6] this situation did not appear for the NP-BMP2 system this being
consistent with the electrokinetic behavior that did not show the presence of protein near surface
The literature offers some examples with reduced short-term release of BMP-2 using more
hydrophilic PLGA-PEG co-polymers [16] or a different synthesis process [25]
(A) (B)
Figure 5 (A) Cumulative release of rhBMP-2 for NP-BMP2 (black square) and NP-BSA-BMP2 (red
circle) systems and cumulative release of BSA for NP-BSA-BMP2 (blue triangle) system incubated
for different times at 37 degC in saline phosphate buffer (pH 74) (B) SDS-PAGE analysis under reducing
0 20 40 60 80 100 120 140 160 180
0
20
40
60
80
100
Cum
ulat
ive
rele
ase
()
Time (hours)
Figure 5 (A) Cumulative release of rhBMP-2 for NP-BMP2 (black square) and NP-BSA-BMP2 (redcircle) systems and cumulative release of BSA for NP-BSA-BMP2 (blue triangle) system incubated fordicrarrerent times at 37 C in saline phosphate bucrarrer (pH 74) (B) SDS-PAGE analysis under reducingconditions of solid fraction of NP-BSA-BMP2 after release at dicrarrerent times where the number of eachlane corresponds to the time in hours
33 Biological Activity and Interactions
331 Cell Migration
Cell migration is the first and necessary step in tissue regeneration [52] Thus a regenerativeagent must accelerate cell migration or at least not interfere with it In the present study we found nodicrarrerences between the groups doses and control in terms of closure of a scratched area (ANOVAwith Tukey multiple comparisons test) (Figure 6) In contrast to our findings previously publisheddata suggests a positive ecrarrect of BMP-2 on cell migration [5354] However in those studies the dosesapplied and the cell types were dicrarrerent than in the current experiments We used lower doses ofBMP-2 in order to test whether even at low dosages BMP-2 could still provide benefits if protectedin a nanoparticle system As mentioned we demonstrated no negative ecrarrect of the system on cellmigration Our results nonetheless support the idea that BMP-2 activity is mediated by the activation ofthe phosphoinositide 3-kinase (PI3K) pathway a common group of signaling molecules that participatein several process with BMP-2 and other molecules [2654] It should also be mentioned that thetimeframe of a migration assay is short Thus the potential advantages of a controlled-release systemas the one under study might be limited That is the release of BMP-2 from the nanoparticles asdemonstrated in Figure 5 is limited to the first 48 h Thus a sustained positive ecrarrect on migrationactivity over time could be hypothesized
Pharmaceutics 2019 11 388 12 of 18Pharmaceutics 2019 11 x 12 of 18
Figure 6 Migration assay Percentage of scratched area closure at 24 and 48 h on different groups and doses
332 Cell Proliferation
Proliferation is another of the cell activities required for tissue regeneration However this property must be balanced with both migration and differentiation and not all three characteristics increase at the same time and with the same ratios [55] In fact reportedly when a dose of BMP-2 induces higher proliferation it decreases differentiation [56] This property has been extensively analyzed but discrepancies can still be detected in the literature Therefore Kim et al analyzed different doses of BMP-2 and its effect on cell proliferation and apoptosis It was confirmed in vitro that high doses but still lower than those used clinically reduce cell proliferation and increase apoptosis [57] This should be avoided We have found that although free BMP-2 does not induce higher proliferation than the control at any of the doses applied nor time points (ANOVA with Tukey multiple comparisons test) the same amount of BMP-2 encapsulated or adsorbed onto PLGA nanoparticles boosts proliferation this being statistically significant when using a dose of 25 ngmL or higher (ANOVA with Tukey multiple comparisons test) (Figure 7) These dosages are still lower than those suggested in previous studies Apart from that difference a positive effect on proliferation was still achieved Moreover following the release pattern from Figure 5 more BMP-2 is expected to be released over time beyond the 7-day time frame Thus a sustained induction effect could be expected as well until full confluency of the cell culture
0
10
20
30
40
50
60
70
80
90
100
0 h 24 h 48 h
o
f scr
atch
ed a
rea
Time point
Control
BMP2-125 ngmL
BMP2-25 ngmL
BMP2-5 ngmL
NP-BMP2-125 ngmL
NP-BMP2-25 ngmL
NP-BMP2-5 ngmL
NP-BSA-BMP2-125 ngmL
NP-BSA-BMP2-25 ngmL
NP-BSA-BMP2-5 ngmL
Figure 6 Migration assay Percentage of scratched area closure at 24 and 48 h on dicrarrerent groupsand doses
332 Cell Proliferation
Proliferation is another of the cell activities required for tissue regeneration However this propertymust be balanced with both migration and dicrarrerentiation and not all three characteristics increaseat the same time and with the same ratios [55] In fact reportedly when a dose of BMP-2 induceshigher proliferation it decreases dicrarrerentiation [56] This property has been extensively analyzed butdiscrepancies can still be detected in the literature Therefore Kim et al analyzed dicrarrerent doses ofBMP-2 and its ecrarrect on cell proliferation and apoptosis It was confirmed in vitro that high doses butstill lower than those used clinically reduce cell proliferation and increase apoptosis [57] This shouldbe avoided We have found that although free BMP-2 does not induce higher proliferation than thecontrol at any of the doses applied nor time points (ANOVA with Tukey multiple comparisons test)the same amount of BMP-2 encapsulated or adsorbed onto PLGA nanoparticles boosts proliferationthis being statistically significant when using a dose of 25 ngmL or higher (ANOVA with Tukeymultiple comparisons test) (Figure 7) These dosages are still lower than those suggested in previousstudies Apart from that dicrarrerence a positive ecrarrect on proliferation was still achieved Moreoverfollowing the release pattern from Figure 5 more BMP-2 is expected to be released over time beyondthe 7-day time frame Thus a sustained induction ecrarrect could be expected as well until full confluencyof the cell culture
Pharmaceutics 2019 11 388 13 of 18Pharmaceutics 2019 11 x 13 of 18
Figure 7 Proliferation of human mesenchymal stromal cells (MSCs) as measured by sulphorhodamine (SRB) absorbance Results were normalized to T0 in each group
333 Osteogenic Differentiation
It has been confirmed that cell differentiation induced by BMP-2 needs the presence of permissive osteoinductive components Particularly β-glycerophosphate has been shown to exert a synergistic effect with BMP-2 in inducing cell differentiation [56] Thus to test for osteogenic differentiation we analyzed the expression of ALP mRNA Maximum ALP activity was found to occur 10 days after stimulation with PLGA-based microparticles containing BMP-2 in co-encapsulation with human serum albumin [16] Although other tests could have been used to reinforce our findings ALP is known to modulate the deposition of mineralized nodules thus indicating osteoblastic activity For all of this we supplemented the differentiation media with β-glycerophosphate and either free BMP-2 NP-BMP2 or NP-BSA-BMP2 for 4 and 7 days so that we could capture the early dynamics of the expression of the gene In our study we identified an increase in the expression of ALP in all groups from day 4 to day 7 (Figure 8) Although ALP at day 7 in the BMP-2 group appears to be higher than for the other two groups the change did not prove significant In fact differences between groups were not statistically significant within any time period Noteworthy though the increase was not significant within the BMP-2 group (p = 0141 Studentrsquos t test) but it was significant within the other two groups (p = 0025 and p = 0003 NP-BMP2 and NP-BSA-BMP2 groups respectively) This again could be taken as a confirmation of the sustained release of the protein from the nanoparticle system beyond the earlier time points
This and both the migration and proliferation studies described below lead us to confirm that the system proposed can maintain a proper release of BMP-2 over time sustaining a positive effect on cell migration and proliferation with initial reduced doses of BMP-2 The fact that the excessive initial burst is prevented is important for the application of this nanotechnology in bone regeneration as in dentistry In this way the negative effects of initial high doses of BMP-2 are avoided at the same time as the molecule is protected from denaturalization inside the NP Thus the regenerator effects are maintained over time
T0 T1 T2 T3 T4 T6 T70
2
4
6
Time point
Nor
mal
ized
Abs
orba
nce
NP-BSA-BMP2-125 ngmL
NP-BSA-BMP2-25 ngmL
NP-BSA-BMP2-5 ngmL
NP-BMP2-125 ngmL
NP-BMP2-25 ngmL
NP-BMP2-5 ngmL
BMP2-125 ngmL
BMP2-25 ngmL
BMP2-5 ngmL
Control
Figure 7 Proliferation of human mesenchymal stromal cells (MSCs) as measured by sulphorhodamine(SRB) absorbance Results were normalized to T0 in each group
333 Osteogenic Dicrarrerentiation
It has been confirmed that cell dicrarrerentiation induced by BMP-2 needs the presence of permissiveosteoinductive components Particularly -glycerophosphate has been shown to exert a synergisticecrarrect with BMP-2 in inducing cell dicrarrerentiation [56] Thus to test for osteogenic dicrarrerentiation weanalyzed the expression of ALP mRNA Maximum ALP activity was found to occur 10 days afterstimulation with PLGA-based microparticles containing BMP-2 in co-encapsulation with human serumalbumin [16] Although other tests could have been used to reinforce our findings ALP is known tomodulate the deposition of mineralized nodules thus indicating osteoblastic activity For all of this wesupplemented the dicrarrerentiation media with -glycerophosphate and either free BMP-2 NP-BMP2 orNP-BSA-BMP2 for 4 and 7 days so that we could capture the early dynamics of the expression of thegene In our study we identified an increase in the expression of ALP in all groups from day 4 to day 7(Figure 8) Although ALP at day 7 in the BMP-2 group appears to be higher than for the other twogroups the change did not prove significant In fact dicrarrerences between groups were not statisticallysignificant within any time period Noteworthy though the increase was not significant within theBMP-2 group (p = 0141 Studentrsquos t test) but it was significant within the other two groups (p = 0025and p = 0003 NP-BMP2 and NP-BSA-BMP2 groups respectively) This again could be taken as aconfirmation of the sustained release of the protein from the nanoparticle system beyond the earliertime points
This and both the migration and proliferation studies described below lead us to confirm that thesystem proposed can maintain a proper release of BMP-2 over time sustaining a positive ecrarrect on cellmigration and proliferation with initial reduced doses of BMP-2 The fact that the excessive initialburst is prevented is important for the application of this nanotechnology in bone regeneration asin dentistry In this way the negative ecrarrects of initial high doses of BMP-2 are avoided at the sametime as the molecule is protected from denaturalization inside the NP Thus the regenerator ecrarrects aremaintained over time
Pharmaceutics 2019 11 388 14 of 18
14 of 18
4 Conclusions
In this work a delivery PLGA-nanosystem previously developed for model proteins was chosen as the reference system to carry and deliver the growth factor BMP-2 This NP system with a dual size distribution was developed following a double-emulsion formulation in which the process and the components used were optimized to reach the appropriate colloidal and biological behavior Encapsulation and adsorption are two different processes to load BMP-2 in PLGA NPs Both were tested to elucidate the factors controlling them and their influence in the physico-chemical and biological properties of nanosystems We verified that proteinndashpolymer specific interactions have a major role in the way that protein molecules are carried and delivered from NPs In vitro experiments showed that BMP-2-loaded PLGA NPs are the nanocarriers with the best release profile over the short-term without an initial burst and with moderate and sustained release of active protein before the onset of polymer degradation Therefore the biological activity is positive with no negative interaction with migration or proliferation but rather the induction of cell differentiation through the expression of ALP
Supplementary Materials The following are available online at wwwmdpicomxxxs1 Figure S1 Scheme of the formulation of NP-BMP2 Figure S2 Scheme of the protein adsorption process for NP-BSA-BMP2 Video S1 NTA experiments for NP-BMP2 Video S2 NTA experiments for empty NPs
Author Contributions Conceptualization JMP-G and PG-M methodology JMP-G ABJ-R and MP-M investigation TdC-S IO-O JMP-G ABJ-R and MP-M resources ABJ-R PG-M FO-R writingmdashoriginal draft preparation JMP-G and MP-M writingmdashreview and editing JMP-G MP-M ABJ-R TdC-S supervision JMP-G PG-M and FO-R funding acquisition ABJ-R and PG-M
Funding This research was funded by the Consejeriacutea de Economiacutea Innovacioacuten Ciencia y Empleo de la Junta de Andaluciacutea (Spain) through research groups FQM-115 and CTS-1028 by the following research project MAT2013-43922-RmdashEuropean FEDER support includedmdash(MICINN Spain) and by MIS Ibeacuterica SL
Acknowledgments The authors wish to express their appreciation for the technical support to D Dariacuteo Abril-Garciacutea
Conflicts of Interest The authors declare no conflict of interest
Figure 8 Relative fold change in the expression of ALP mRNA (control group BMP2 at 4 days) =Statistical significance of the comparison over time (p = 0025 and p = 0003 Studentrsquos t test NP-BMP2and NP-BSA-BMP2 groups)
4 Conclusions
In this work a delivery PLGA-nanosystem previously developed for model proteins was chosenas the reference system to carry and deliver the growth factor BMP-2 This NP system with a dualsize distribution was developed following a double-emulsion formulation in which the processand the components used were optimized to reach the appropriate colloidal and biological behaviorEncapsulation and adsorption are two dicrarrerent processes to load BMP-2 in PLGA NPs Both were testedto elucidate the factors controlling them and their influence in the physico-chemical and biologicalproperties of nanosystems We verified that proteinndashpolymer specific interactions have a major role inthe way that protein molecules are carried and delivered from NPs In vitro experiments showed thatBMP-2-loaded PLGA NPs are the nanocarriers with the best release profile over the short-term withoutan initial burst and with moderate and sustained release of active protein before the onset of polymerdegradation Therefore the biological activity is positive with no negative interaction with migrationor proliferation but rather the induction of cell dicrarrerentiation through the expression of ALP
Supplementary Materials The following are available online at httpwwwmdpicom1999-4923118388s1Figure S1 Scheme of the formulation of NP-BMP2 Figure S2 Scheme of the protein adsorption process forNP-BSA-BMP2 Video S1 NTA experiments for NP-BMP2 Video S2 NTA experiments for empty NPs
Author Contributions Conceptualization JMP-G and PG-M methodology JMP-G ABJ-R andMP-M investigation TdC-S IO-O JMP-G ABJ-R and MP-M resources ABJ-R PG-M FO-Rwritingmdashoriginal draft preparation JMP-G and MP-M writingmdashreview and editing JMP-G MP-MABJ-R TdC-S supervision JMP-G PG-M and FO-R funding acquisition ABJ-R and PG-M
Funding This research was funded by the Consejeriacutea de Economiacutea Innovacioacuten Ciencia y Empleo de la Juntade Andaluciacutea (Spain) through research groups FQM-115 and CTS-1028 by the following research projectMAT2013-43922-RmdashEuropean FEDER support includedmdash(MICINN Spain) and by MIS Ibeacuterica SL
Acknowledgments The authors wish to express their appreciation for the technical support to D DariacuteoAbril-Garciacutea
Conflicts of Interest The authors declare no conflict of interest
Pharmaceutics 2019 11 388 15 of 18
References
1 Van Rijt S Habibovic P Enhancing regenerative approaches with nanoparticles J R Soc Interface 2017 14[CrossRef]
2 Kumar B Jalodia K Kumar P Gautam HK Recent advances in nanoparticle-mediated drug delivery JDrug Deliv Sci Technol 2017 41 260ndash268 [CrossRef]
3 Mir M Ahmed N Rehman AUR Recent applications of PLGA based nanostructures in drug deliveryColloids Surf B Biointerfaces 2017 159 217ndash231 [CrossRef] [PubMed]
4 Jana S Jana S Natural polymeric biodegradable nanoblend for macromolecules delivery In RecentDevelopments in Polymer Macro Micro and Nano Blends Woodhead Publishing Cambridge UK 2017pp 289ndash312 ISBN 9780081004081
5 Danhier F Ansorena E Silva JM Coco R Le Breton A Preacuteat V PLGA-based nanoparticles Anoverview of biomedical applications J Control Release 2012 161 505ndash522 [CrossRef] [PubMed]
6 Ding D Zhu Q Recent advances of PLGA micronanoparticles for the delivery of biomacromoleculartherapeutics Mater Sci Eng C 2018 92 1041ndash1060 [CrossRef] [PubMed]
7 Arias JL Unciti-Broceta JD Maceira J del Castillo T Hernaacutendez-Quero J Magez SSoriano M Garciacutea-Salcedo JA Nanobody conjugated PLGA nanoparticles for active targeting of AfricanTrypanosomiasis J Control Release 2015 197 190ndash198 [CrossRef]
8 Giteau A Venier-Julienne MC Aubert-Poueumlssel A Benoit JP How to achieve sustained and completeprotein release from PLGA-based microparticles Int J Pharm 2008 350 14ndash26 [CrossRef]
9 Fredenberg S Wahlgren M Reslow M Axelsson A The mechanisms of drug release inpoly(lactic-co-glycolic acid)-based drug delivery systemsmdashA review Int J Pharm 2011 415 34ndash52[CrossRef]
10 White LJ Kirby GTS Cox HC Qodratnama R Qutachi O Rose FRAJ Shakeshecrarr KM Acceleratingprotein release from microparticles for regenerative medicine applications Mater Sci Eng C 2013 332578ndash2583 [CrossRef]
11 Ortega-Oller I del Castillo-Santaella T Padial-Molina M Galindo-Moreno P Joacutedar-Reyes ABPeula-Garciacutea JM Dual delivery nanosystem for biomolecules Formulation characterization and in vitrorelease Colloids Surf B Biointerfaces 2017 159 586ndash595 [CrossRef]
12 McClements DJ Encapsulation protection and delivery of bioactive proteins and peptides usingnanoparticle and microparticle systems A review Adv Colloid Interface Sci 2018 253 1ndash22 [CrossRef]
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14 Bapat RA Joshi CP Bapat P Chaubal TV Pandurangappa R Jnanendrappa N Gorain B Khurana SKesharwani P The use of nanoparticles as biomaterials in dentistry Drug Discov Today 2019 24 85ndash98[CrossRef]
15 Ji Y Xu GP Zhang ZP Xia JJ Yan JL Pan SH BMP-2PLGA delayed-release microspheres compositegraft selection of bone particulate diameters and prevention of aseptic inflammation for bone tissueengineering Ann BioMed Eng 2010 38 632ndash639 [CrossRef] [PubMed]
16 Kirby GTS White LJ Rahman CV Cox HC Qutachi O Rose FRAJ Hutmacher DWShakeshecrarr KM Woodrucrarr MA PLGA-Based Microparticles for the Sustained Release of BMP-2 Polymers2011 3 571ndash586 [CrossRef]
17 Qutachi O Shakeshecrarr KM Buttery LDK Delivery of definable number of drug or growth factor loadedpoly(dl-lactic acid-co-glycolic acid) microparticles within human embryonic stem cell derived aggregates JControl Release 2013 168 18ndash27 [CrossRef] [PubMed]
18 Wang Y Wei Y Zhang X Xu M Liu F Ma Q Cai Q Deng X PLGAPDLLA core-shell submicronspheres sequential release system Preparation characterization and promotion of bone regeneration in vitroand in vivo Chem Eng J 2015 273 490ndash501 [CrossRef]
Pharmaceutics 2019 11 388 16 of 18
19 Zhang H-X Zhang X-P Xiao G-Y Hou -Y Cheng L Si M Wang S-S Li Y-H Nie L In vitroand in vivo evaluation of calcium phosphate composite scacrarrolds containing BMP-VEGF loaded PLGAmicrospheres for the treatment of avascular necrosis of the femoral head Mater Sci Eng C 2016 60 298ndash307[CrossRef]
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23 Padial-Molina M de Buitrago JG Sainz-Urruela R Abril-Garcia D Anderson P OrsquoValle FGalindo-Moreno P Expression of Musashi-1 during osteogenic dicrarrerentiation of oral MSC An in vitro studyInt J Mol Sci 2019 20 2171 [CrossRef] [PubMed]
24 DrsquoAngelo I Garcia-Fuentes M Parajoacute Y Welle A Vaacutentus T Horvaacuteth A Boumlkoumlnyi G Keacuteri GAlonso MJ Nanoparticles based on PLGApoloxamer blends for the delivery of proangiogenic growthfactors Mol Pharm 2010 7 1724ndash1733 [CrossRef] [PubMed]
25 Chang H-C Yang C Feng F Lin F-H Wang C-H Chang P-C Bone morphogeneticprotein-2 loaded poly(DL-lactide-co-glycolide) microspheres enhance osteogenic potential ofgelatinhydroxyapatite-tricalcium phosphate cryogel composite for alveolar ridge augmentation JFormos Med Assoc 2017 116 973ndash981 [CrossRef] [PubMed]
26 Padial-Molina M Volk SL Rios HF Periostin increases migration and proliferation of humanperiodontal ligament fibroblasts challenged by tumor necrosis factor -crarr and Porphyromonas gingivalislipopolysaccharides J Periodontal Res 2014 49 405ndash414 [CrossRef] [PubMed]
27 Liang C-C Park AY Guan J-L In vitro scratch assay A convenient and inexpensive method for analysisof cell migration in vitro Nat Protoc 2007 2 329ndash333 [CrossRef]
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31 Lochmann A Nitzsche H von Einem S Schwarz E Maumlder K The influence of covalently linked andfree polyethylene glycol on the structural and release properties of rhBMP-2 loaded microspheres J ControlRelease 2010 147 92ndash100 [CrossRef]
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Pharmaceutics 2019 11 388 17 of 18
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Pharmaceutics 2019 11 388 18 of 18
56 Hrubi E Imre L Robaszkiewicz A Viraacuteg L Kereacutenyi F Nagy K Varga G Jenei A Hegeduumls CDiverse ecrarrect of BMP-2 homodimer on mesenchymal progenitors of dicrarrerent origin Hum Cell 2018 31139ndash148 [CrossRef]
57 Kim HKW Oxendine I Kamiya N High-concentration of BMP2 reduces cell proliferation and increasesapoptosis via DKK1 and SOST in human primary periosteal cells Bone 2013 54 141ndash150 [CrossRef]
copy 2019 by the authors Licensee MDPI Basel Switzerland This article is an open accessarticle distributed under the terms and conditions of the Creative Commons Attribution(CC BY) license (httpcreativecommonsorglicensesby40)
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12ANEXO DE ORIGINALIDAD