PREPARED BY KYLE SUNG
AUTOMATED NUCLEIC ACID ISOLATION SYSTEMS
®
PRODUCT INTRODUCTION
Automated Nucleic Acid Isolation Systems
®
PRODUCT DESIGN
+
Design
Industrial Design
Electrical
Thermal Control
+
Feedback Control
+
PCBA
Mechanical Design
INTENDED USE OF SOLMAG®
Magnetic Bead Separation: Bench-top automated system for quick purification of nucleic acids from bio-
specimens using magnetic bead separation technology.
Liquid Handling System: Liquid handling automation, using pre-filled reagent cartridges
For research and in vitro diagnosis use
FUNCTION PRINCIPLE
Advanced Liquid Handling Magnetic Bead Separation
Sample Material Lysis Step Binding Step
Magnetic Separation Washing Step Elution Step; Purified Nucleic Acid
CORE TECHNOLOGIES
The Solis-Charger® Technology
The Solis-ChargerR Technology is a novel magnetic bead-based technology that provides a switchable surface charge dependent on the pH and salt concentration of the surrounding buffer to facilitate nucleic acid purification. In low pH conditions, the magnetic beads have a positive charge that binds the negatively charged nucleic acid backbone (see figure1 below). Proteins and other contaminants are not bound and are simply washed away in an aqueous wash buffer. To elute nucleic acids, the charge on the surface of the bead is neutralized by raising the pH to 8.0 using a low salt elution buffer (see figure below). Purified nucleic acid elutes instantly into this elution buffer, and is ready for use in downstream applications.
CORE TECHNOLOGIES
The 2D CatcherMag® Technology for Sample Preparation
The 2D CatcherMag® technology is a novel magnetic bead-based modification technology. The 2D CatcherMag® is modified and designed for different sample types to optimized collection/extraction result. With the nano-scale modification on the 2D CatcherMag®, we can provide the users a better solution while collecting the samples for reliable polymerase chain reaction results and accurate data analysis.
The 2D CatcherMag® adhering to pathogen in the collection tube
SOLMAG ® 12
Automated Nucleic Acid Purification Systems
SolMag®12: Compact Bench-top Instrument Process 1-12 samples simultaneously 7 inches touch screen Light: Total weight less than 42 Kg Fast: Up to 12 results in 35 minutes Precise liquid volume and temperature control Extremely easy to operate and maintain
SOLMAG ® 12
Front View of Solmag®12
Transparent Maintenance WindowThe Transparent Window is designed to let the user have a clean and sharp look inside the system during operation.
Seven Inches Full-ColoredThe full-colored touch-screen will simplified and guide you through the setup procedure.
Reagent & Plastic Disposable DrawerOpen the drawer to load the ready-to-use prefilled reagent cartridges and other plastic consumables required for performing a nucleic acid purification reaction.Rubber Hand Holders Hand Holders are designed to let users move the instrument with comfort.
SOLMAG ® 12
Inside View of Solmag®12
Six-linked PrefilledReagent Cartridges
Tip Set
Regular Filtered Tip
Small Filtered Tip
Sample TubeTransferrable Sample Rack
Elution Tube
SOLMAG ® 12
Back View of Solmag®12
USB Connection Port
RS232 Connection Port
Power Outlet
Power Switch
SOLMAG ®12 SPECIFICATIONS
Light as feather: SolMag®12 weights only
42kgw, which is 50% lighter in average than other similar throughputs liquid handling extractor in the market.
MagNaPure Compact: 60Kg
Qiagen EZ1 Advanced XL: 48Kg
MagCore HF16: 70Kg
SolMag®12Part No. SVP0101
Dimensions (W x D x H) 637mm x 462mm x 540mm
Weight 42kgw
Interface 7” LCD touch-screen
Processing time 20 – 45mins
Throughput 1-12 Samples per run
Temperature Uniformity ±0.5℃
Liquid Extraction Volume Error Less than 5%
Sample Volume 50ul – 3000ul
Elution Volume 30ul – 200ul
Power Input power :90 ~ 132V&180 ~ 264Vby switch50/60HzOut power :5V60A
Certification CE、IVD、FDA
UNIQUE SPECIFICATIONS
7” Touch Screen: Optimized built-in protocol covers six major applications such as clinical
diagnostic, forensic science, food safety and CFC.
Built-in Template: Up to three preferable
templates can be set up by the users for performing the same routine protocol daily, which can also reduce the risk from human operational error.
UV LAMP DECONTAMINATION
• With a built-in UV-lamp, the UV decontamination guarantee maximum safety for both samples and environment
ABOUT INSTRUMENT
SLEEP MODE
SOFTWARE UPDATE
DEVICES TESTS
UV LAMP
SOLMAG ® 24
Automated Nucleic Acid Isolation Systems
SolMag® 24:The SolMag®24 provides all the advantages of the SolMag®12, and increases the throughput to 24 samples per run. This new workstation has been developed to combine the convenience and reliability of the SolMag® Series with the need for low-to-medium throughput.
SOLMAG ISOLATION KITS
Ready-to-use pre-filled, Reagent Cartridge: The SolMag® Nucleic Acid Isolation kits contain all reagents and
disposables needed for 48 nucleic acid isolation reactions.
SolMag® NA Isolation Kits
1. Blood/Tissue Nucleic Acid Isolation Kit
2. Virus Nucleic Acid Isolation Kit
3. Pathogen Nucleic Acid Isolation Kit
4. Food Safety Nucleic Acid Isolation Kit
5. Forensic Science DNA Isolation Kit
6. Circulation Nucleic Acid Isolation Kit
7. Virus Nucleic Acid Isolation Kit - Large Volume
WORK STATION
Mid to high throughputs• >= 24 preps/run
Programmable robot arm controlled by separated computerBig instrument dimension / heavy weightComplicated setup procedures /require additional training before operationHigher instrument and consumable cost
• > US$ 80KEx:
• Qiasymphony(Qiagne)• Cobus (Roche)
STANDALONE
Small to mid throughputs
• < 24 preps/run Built-in micro-processor and protocols, no PC required
Small Footprint / compact
Easy setup and operation steps
Lower instrument and consumable cost
• <US$10KEx:
• SolMag (Solison), EZ1 Advanced (Qiagen), MagNa Pure Compact (Roche), iPrep (Invitrogen) … etc
COMPARISON WITH WORK STATION
Advantage• Fully walk away automation:
minimum procedures of set up, which require three simple steps.
• Enable to handle a wide range of volumes on starting materials and elutes
• Better extraction qualityDisadvantage
• Bigger instrument size and heavier weight
• Higher instrument costEx:
• SolMag (Solison), EZ1 Advanced (Qiagen), MagNa Pure Compact (Roche), iPrep (Invitrogen) … etc
MAGNETIC ROD
Advantage• Lower kit price
Disadvantage• Easy to have magnetic bead
residues in eluted products• Limited or fixed sample / elute
volume handling choice• Semi-Automation: tedious
manual set up procedure required, kills time and cause humor errors.
Ex:• Maxwell (Promega)• KingFisher (Thermo Fisher)
LIQUID HANDALING
COMPARISON WITH MAGNETIC ROD SYSTEM
THANK YOU FOR YOUR ATTENTION!!
Solison Biotechnology Corp.Web: www.solisonbio.comAdd: No. 3-2, Chien-kuo road, TEPZ Tantzu, 427 Taichung, Taiwan R.O.CTel: +886 4 2531 1334 Fax: +886 2 2531 1343Mail: [email protected]