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WinFLOW Software V4.2
Operators Manual
151 Graham Road P.O. Box 9010 College Station, Texas 77842-9010
Telephone (979) 690-1711 FAX (979) 690-0440 www.oico.com [email protected]
Flow SolutionIVSystem
Flow Solution3100 System
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ii
Notice
The information contained in this document may be revised without notice.
OI Analytical shall not be liable for errors contained herein or for incidental or
consequential damages in connection with the furnishing, performance, or use of this
material.
No part of this document may be reproduced, photocopied, or translated to another
language without the prior written consent of OI Analytical.
Rev. 2.3 July 2007
OI Analytical Part Number A001965
Printed in the U.S.A.
Publication 134800707
Copyright 20052007 OI Analytical
Adobe and Acrobat Reader are registered trademarks of Adobe Systems, Inc.
Windows is a registered trademark of Microsoft Corporation.CNSolution, Flow Solution, and WinFLOW are trademarks or registered trademarks of OI Analytical.
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iii
Limited Warranty
OI Analytical warrants each OI Analytical manufactured product against defects in
materials and workmanship under normal use and service for a period of one year.
Equipment installed by OI Analytical is warranted from the installation date; all other
equipment is warranted from the ship date. If purchaser schedules or delays installation
more than 90 days after delivery, then the warranty period starts on the 91st day from
date of shipment. This warranty extends only to the original purchaser. OI Analyticalwill, at its option, repair or replace equipment that proves to be defective during the
warranty period, provided the equipment is returned to OI Analytical at the expense of
the purchaser.
Consumables, expendables, and parts are warranted for 30 days and are not covered
under extended warranties or service contracts.
OI Analytical warrants for a period of one year from the date of delivery: (i) the
Software, when installed and used with an OI Analytical recommended hardware
configuration, will perform in substantial conformance with the documentation
supplied with the Software; and (ii) the physical media on which the Software is
furnished will be free from defects in materials and workmanship under normal use.
This warranty shall not apply to defects originating from, but not limited to, the
following:
Improper maintenance or operation by the purchaser;
Purchaser supplied accessories or consumables;
Modification or misuse by the purchaser;
Operation outside the products environmental and electrical specifications;
Software, interfacing, parts, or supplies not supplied by OI Analytical;
A computer not meeting the minimum specifications recommended by OI
Analytical;
Improper or inadequate site preparation;
Purchaser induced contamination or leaks.
THE FOREGOING WARRANTY IS IN LIEU OF ALL OTHER WARRANTIES,
EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO ANYWARRANTY OF MERCHANTABILITY, FITNESS, OR ADEQUACY FOR ANY
PARTICULAR PURPOSE OR USE. OI ANALYTICAL SHALL NOT BE LIABLE
FOR ANY SPECIAL, INCIDENTAL, OR CONSEQUENTIAL DAMAGES,
WHETHER IN CONTRACT, TORT, OR OTHERWISE.
Any service requests or questions should be directed to the OI Analytical Customer
Support Center at (800) 336-1911 or (979) 690-1711.
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iv
Safety Information
For operator safety, pay special attention to the WARNINGand CAUTIONstatements
throughout the manual.
A WARNINGindicates a condition or possible situation that could result in
physical injury to the operator.
A CAUTIONindicates a condition or possible situation that could damage ordestroy the product or the operators work.
Warnings and precautions in this manual or on the instrument must be followed during
operation, service, and repair of the instrument. Failure to follow these warnings and
precautions violates the safety design standards and intended use of the instrument.
OI Analytical is not liable for the operators failure to comply with warnings and
precautions.
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v
Table of Contents
Notice .............................................................................................................................. ii
Limited Warranty ........................................................................................................ iii
Safety Information ....................................................................................................... iv
Chapter 1: Introduct ion ........................................................1Features .......................................................................................................................... 1
Computer Requirements .............................................................................................. 2
Security Settings for WindowsXP Operating Systems...........................................2
Chapter 2: Instal lat ion ..........................................................4Installing WinFLOW.................................................................................................... 4
Security Settings for WindowsXP Operating Systems ........................................... 5
Chapter 3: Configurat ion .....................................................7Configuring WinFLOW ............................................................................................... 7
Configuring the RA Sampler ....................................................................................... 9
Vial Depths..............................................................................................................10
Dilutor Setup ........................................................................................................... 12
Wash and Diluent ....................................................................................................13
Rack Placement .......................................................................................................14
Rack Editor..............................................................................................................15
Configuring the 3360 Sampler................................................................................... 18
Vial Depths..............................................................................................................19
Dilutor Setup ........................................................................................................... 20
Wash and Diluent ....................................................................................................21
Rack Placement .......................................................................................................21
Peck Probe during Rinse .........................................................................................22
Configuring the 3090 Sampler................................................................................... 22
Vial Depths..............................................................................................................23
Wash and Diluent ....................................................................................................24
Rack Placement .......................................................................................................25
Peck Probe during Rinse .........................................................................................25
Chapter 4: Introduction to WinFLOW ...............................26Starting WinFLOW .................................................................................................... 26
Select View Dialog Box .......................................................................................... 26
Tools Menu................................................................................................................... 27Preferences .............................................................................................................. 28
Configuration...........................................................................................................28
Events Scheduler .....................................................................................................29
Instrument Control...................................................................................................30
Pump Timer.............................................................................................................30
Window Menu ............................................................................................................. 30
About Menu ................................................................................................................. 31
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vi
Shortcut Keys .............................................................................................................. 31
Icon Ribbon.................................................................................................................. 31
System Security ........................................................................................................... 33
WinFLOW Quick Start .............................................................................................. 34
Chapter 5: Method Editor ...................................................36General Description .................................................................................................... 36
Method Editor Icon Ribbon ..................................................................................... 37
Opening a Method ...................................................................................................37
Creating a Method ...................................................................................................38
Saving a Method......................................................................................................38
Flow Control Settings Screen ..................................................................................... 38
Instrument................................................................................................................39
Sampling Mode .......................................................................................................39
CCV/Drift Fail.........................................................................................................41
Vial Types ............................................................................................................... 41
Off-scale (HI) Samples............................................................................................41
Insert Baselines........................................................................................................42
Pump Control...........................................................................................................42Detector/Channel Settings Screen ............................................................................. 43
Channels .................................................................................................................. 43
Name .......................................................................................................................43
Methodology ........................................................................................................... 44
CAS Number ........................................................................................................... 44
Detector Settings .....................................................................................................44
Channel Subtractions...............................................................................................45
Signal Filter and Marking Settings Screen............................................................... 46
Smoothing ............................................................................................................... 47
Baseline Marking.....................................................................................................48
Inverted Chemistry..................................................................................................48
Peak Marking...........................................................................................................48Calibration/Quantitation Settings Screen................................................................. 50
Calibration............................................................................................................... 51
Quantitation............................................................................................................. 51
Flagging...................................................................................................................52
Overlimit Samples...................................................................................................52
Timed Events Editor Screen....................................................................................... 52
B (Basis of Timing).................................................................................................53
Time.........................................................................................................................53
Device......................................................................................................................53
# (Selector) .............................................................................................................. 54
State .........................................................................................................................54
Calibrants Table Editor Screen ................................................................................. 55Calibrants.................................................................................................................55
Independent Calibration Verification ......................................................................56
Continuing Calibration Verification........................................................................56
Spike........................................................................................................................56
Stock Calibrants.......................................................................................................57
Drift Correction .......................................................................................................57
Verifying Method Settings .......................................................................................... 57
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vii
Chapter 6: Sample Table....................................................58Cup Number ............................................................................................................ 59
Sample Names.........................................................................................................60
Sample Type............................................................................................................60
Replicate..................................................................................................................61
Dilution and Weight Factors ...................................................................................61
Vial Type.................................................................................................................61
Comments................................................................................................................61Sample Table Menu Options ...................................................................................... 62
File Menu ................................................................................................................ 62
Edit Menu................................................................................................................ 62
Tools and Window Menus.......................................................................................63
Creating a Sample Table............................................................................................. 63
Synchronization Peak (SYNC)................................................................................63
Carryover Correction (CO)......................................................................................63
Calibrants.................................................................................................................63
Calibrants To Be Diluted.........................................................................................63
Unknowns To Be Diluted........................................................................................64
Stock Solutions........................................................................................................64
Incremental Sensitivity Drift Correction .................................................................65Total Sensitivity Drift Correction............................................................................65
Independent Calibration Verification ......................................................................65
Continuing Calibration Verification........................................................................65
Spiked Samples .......................................................................................................66
Blanks and Baseline Samples..................................................................................66
Autodilution ................................................................................................................. 67
Import and Export Functions .................................................................................... 68
Exporting a Table ....................................................................................................68
Importing a Table ....................................................................................................68
Chapter 7: Data Collection .................................................69Initiating Data Collection ........................................................................................... 69
Monitoring the Baseline.............................................................................................. 72
Starting a Sample Analysis......................................................................................... 73
Peak Plot Screen......................................................................................................73
Calibration Plot Screen............................................................................................73
Calibration Results Screen ...................................................................................... 74
Peak Table Results Screen.......................................................................................75
Adding Samples to a Sample Table.........................................................................75
Modifying a Sample Table During a Run ................................................................. 76
Stopping a Run ............................................................................................................ 76
Operator-Stopped Run.............................................................................................76
WinFLOW-Stopped Run.........................................................................................76
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viii
Chapter 8: Results and Analys is .......................................77Loading a Data File..................................................................................................... 77
Data Processing Tools ................................................................................................. 78
Data Analysis Screen...............................................................................................78
Grid On/Off Icon .....................................................................................................78
Peak Editor Icon ......................................................................................................78
Recalculate Icon ......................................................................................................78
Delete/Reprocess Peaks Icon...................................................................................79Menu Options .......................................................................................................... 80
Peak Plot Screen.......................................................................................................... 82
Magnifying the Peak Plot ........................................................................................ 82
Using the Cursor For Peak Identification................................................................83
Changing a Peak State (Add, Delete, Toggle Baseline Point).................................83
Calibration Plot Screen............................................................................................... 84
Magnifying the Calibration Plot..............................................................................84
Using the Cursor for Calibration Point Identification .............................................84
Removing Outlier Calibration Points ...................................................................... 84
Peak Results Table Screen.......................................................................................... 85
Peak Results Table Fields........................................................................................85
Calibration Results Table Screen............................................................................... 87Calibrant ID.............................................................................................................87
Calibration Coefficients...........................................................................................87
Correlation Coefficient............................................................................................87
Carryover.................................................................................................................87
Drift Correction .......................................................................................................87
Reports ......................................................................................................................... 88
Exporting a Report ..................................................................................................... 89
Chapter 9: Troubleshooting...............................................90Instrument Errors....................................................................................................... 90
WinFLOW Errors....................................................................................................... 91Reporting Errors ......................................................................................................92
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WinFLOW Software Operators Manual: Chapter 1 1
Rev. 2.3
Ch a p t e r 1 Introduction
The WinFLOW software is a Windows-based program designed to offer a high
level of automation when used with any of the OI Analytical Flow Solution
systems,as well as many other automated chemistry analyzers.
Features
Automatic startup and shutdown enhances system productivity.*
Pause option permits run and sample table modification anytime during a run.
Sensitivity drift correction eliminates the need to fully recalibrate after ICV/CCV
failure.
Operator-defined dilution factors add operator flexibility in autodilution mode.*
Sample dilution before the run reduces analysis time.
Automatic working calibration standard preparation from stock solutions reduces
operator workload.*
Manual injection mode permits system operation without an autosampler.
Peak editing improves peak height and area marking.
Post-run peak height, area, and digital signal processing algorithms enhance
analyte detectability.
Mean and RSD values add important QA/QC data to reports.
Options provide customized reporting capabilities.
Complete French, German, and Spanish language support provides enhanced
usability.
*This feature requires specific hardware for full system functionality.
The WinFLOW software package includes the following:
CD-ROM containing the following:
WinFLOW Software
Hardware Operators Manual (one of the following):
WinFLOWSoftware Operators Manual
Flow SolutionIV Operators Manual
Flow SolutionIVOperators Manual
Flow Solution3000 Operators Manual
Flow Solution3100 Operators Manual
CNSolutionFS 3100 Operators Manual
Adobe
Acrobat Reader
WinFLOWsoftware serial number (labeled on the outside of the CD-ROM case)
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Introduction
WinFLOW Software Operators Manual: Chapter 1 2
Rev. 2.3
Computer Requirements
The following table contains minimum and recommended computer requirements for
successfully installing and using the WinFLOWsoftware.
NOTE: Using WinFLOWsoftware requires a working knowledge of the Windows
2000 or WindowsXP Pro operating system.
NOTE: Flow SolutionIII and Flow SolutionIV systems larger than three channels
or Flow Solution3000 systems larger than two channels require the
recommended computer requirements.
NOTE: Power management features (excluding the monitor) can cause shutdowns
and errors during analysis.
Securi ty Settings
for WindowsXPOperatingSystems
When using the WinFLOWsoftware with a WindowsXP Pro operating system, set
permissions to Everyonefor full control of all WinFLOWsoftware features. The
default setting for the WindowsXP Pro operating system is NO users. Therefore,
change permissions to Everyone after installing WinFLOWsoftware on the
computer.
1. Enable Full Control to Everyone by right-clicking Startat the screens lower left
corner.
2. Click Explore.
3. Find the Folder named Flow_4.
Table 1.1. Computer requirements
Parameter Minimum Recommended
Type IBM compatible IBM compatible
Processor speed 1 GHz 3 GHz
Memory 512 MB 1 GB or greater
Free disk space 500 MB 1 GB or greater
Operating system Windows2000 or XP
Pro
WindowsXP Pro
Graphics SVGA XGA
COM port One available RS-232 or
USB-to-RS-232 converter
One available RS-232
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Introduction
WinFLOW Software Operators Manual: Chapter 1 3
Rev. 2.3
NOTE: WinFLOW normally installs in this folder. If the software installed in a
different location, go to that folder.
4. Right-click the Flow_4 folder and select Properties.
5. Under the Securitytab, click the Addbutton.
6. In the Select Users, Computers, or Groups dialog box, type the word Everyone
under the Enter the object names to select sectionand click OK(see Figure 1.1).
Figure 1.1. Permissions dialog box
7. Click the checkbox under Allownext to Full Controlto enable full control.
8. Click Applyand OKto exit the screen.
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WinFLOW Software Operators Manual: Chapter 2 4
Rev. 2.3
Ch a p t e r 2 Installation
This chapter presents step-by-step procedures for properly installing WinFLOW.
Installing WinFLOW
The WinFLOW CD contains WinFLOW software, the WinFLOW software manual,
and all instrument operation manuals. Install WinFLOW using the following
instructions:
1. From the Windows environment, insert the WinFLOW CD into the available CD-
ROM drive. The WinFLOW Setup Disc screen appears (Figure 2.1).
Figure 2.1. WinFLOW Setup Disc screen
If the WinFLOW Setup Disc screen does not automatically appear after inserting
the CD:
Click the Windows Startbutton.
Select Run.
Typex:\setup,wherexrepresents the letter assigned to the CD-ROM drive.
Click OKto display the WinFLOW Setup Disc screen.
2. Select the appropriate language and click Installto begin the installation process.
Follow the instructions on the screen.
NOTE: Do not use more than eight characters or spaces in the directory names.
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Installation
WinFLOW Software Operators Manual: Chapter 2 5
Rev. 2.3
3. After WinFLOW installs, click OK. The Configure WinFLOW dialog box appears
(Figure 2.2). Proceed to Chapter 3, Configuration on page 7for details on
configuring WinFLOW.
Figure 2.2. Configure WinFLOW dialog box
Security Settings for WindowsXP Operating Systems
When using WinFLOW with a Windows XP operating system, set permissions to
Everyonefor full control of all of WinFLOW software features. The default setting for
a Windows XP operating system is NO users. Therefore, change permissions toEveryone after installing WinFLOW on the computer.
1. Enable Full Control to Everyone by right-clicking Startat the screens lower left
corner.
2. Click Explore.
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Installation
WinFLOW Software Operators Manual: Chapter 2 6
Rev. 2.3
3. Find the Folder named Flow_4.
NOTE: WinFLOW normally installs in this folder. If the software installed in a
different location, go to that folder.
4. Right-click the Flow_4 folder and select Properties.
5. Click the Securitytab and add the group Everyone if not already created
(Figure 2.3).
Figure 2.3. Permissions dialog box
6. Click the checkbox under Allownext to Full Controlto allow everyone full control
permissions.
7. Click Applyand OKto exit the screen.
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WinFLOW Software Operators Manual: Chapter 3 7
Rev. 2.3
Ch a p t e r 3 Configuration
After installing WinFLOW, configure communications to the analyzer and autosampler.
Configuring WinFLOW
1. Access the Configuration Editor by selecting FLOW config 4.2from the Start
menu. Click Programs, WinFLOW, and then the FLOW config icon (or go to
Tools Configuration in WinFLOW). The Configure WinFLOW dialog box appears
(see Figure 3.1).
Figure 3.1. Configure WinFLOW dialog box
2. Under Configure Software, select the appropriate language for WinFLOW to
display for its titles, buttons, labels, etc. The language options are English, French,
Dutch, and Spanish.
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Configuration
WinFLOW Software Operators Manual: Chapter 3 8
Rev. 2.3
3. Click the Edit Facility Informationbutton to enter a facility name and department
to display on hard-copy reports.
4. Select the Create Debug Filescheckbox to generate text files that assist OI
Analytical support personnel with troubleshooting software and performance
problems.
5. Select the communication port (Com Port), Sampler Control, and the analyzer
type (Platform) to use. Only available communication ports appear as choices.
6. Select the appropriate Sampler Control.
a. Select Standaloneif using an autosampler not listed or if no autosampler is
used. If using an autosampler not listed, the autosamplers timing cycle must
match WinFLOW. The autosampler and data collection must initiate at the
same time.
b. Select RA to use vials and vial racks provided with the RA Sampler. The RA
Sampler supports any combination of three trays: 21, 24, 40, 60, or 90
positions; the RA sampler also supports the Autodilutor.
c. Select 3360to use vials and vial racks provided with the Model 3360 Sampler.
The Model 3360 supports any combination of four 60-position and 90-position
racks; the Model 3360 also supports the Autodilutor.
d. Select 3090to use vials and vial racks provided with the Model 3090 Sampler;
the Model 3090 supports either one 60-position or one 90-position rack.
e. Click the Configure Samplerbutton to set the sampler to utilize up to four
different types of sample cups, configure any dilutor in use, specify the desiredrack options, and define alternate locations for wash and diluent fluids.
7. Check the Auto 502 check box, if appropriate:
If using a Flow Solution IV or IVsystem, check the Auto 502checkbox to control
the Precision Pump through the Instrument Control dialog box or the Auto Events
Scheduler (see Chapter 4, Tools Menu on page 27).
If using an Flow Solution 3100 system, select the Auto 502checkbox to control the
Precision Pump.
The CNSolution FS 3100 uses the Model 3090 Autosampler. Flow Solution 3100
systems use either the Model 3090 or Model 3360 Autosampler. Flow Solution III
and IV systems use any of the samplers, including the RA, Model 3090, and Model
3360. Run upgraded systems without one of the listed autosamplers in the standal-
one sampler control mode, provided the autosampler being used is capable of inde-
pendent operation.
NOTE: Configuring WinFLOW every time the software opens is not necessary.
Change WinFLOW configuration settings only if altering the instrument
configuration.
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Configuration
WinFLOW Software Operators Manual: Chapter 3 9
Rev. 2.3
8. After selecting the checkbox to control the Precision Pump, use the Pump Turns
pulldown menu to select the pump direction (when viewed from the front of the
pump). Leftindicates the negative pump direction (the pump pushes the liquids
clockwise), while Rightindicates the positive direction (counter-clockwise).
Most Flow Solution IV customers will select Right. Most Flow Solution 3100 cus-
tomers will select Left.
Configuring the RA Sampler
1. Select the RA sampler in the Configure WinFLOW dialog box.
2. Click Configure Sampler. The RA Configuration dialog box appears as shown in
Figure 3.2.
Figure 3.2. RA Configuration dialog box
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Configuration
WinFLOW Software Operators Manual: Chapter 3 10
Rev. 2.3
Vial Depths Using a Traveling Wash Reservoi r (TWR)
Adjust the depth that the sample probe travels into a sample cup.
1. Click Vial Depthsin the RA Configuration dialog box. The Vial Depths dialog box
appears (see Figure 3.3).
Figure 3.3. Vial Depths dialog box
2. Enter the appropriate vial depths (in millimeters) in the corresponding boxes. The
maximum probe depth in the sample vial is approximately 95 mm.
Using a Static Wash Reservoir (No TWR)
Adjust the depth that the sample probe travels into a sample cup.
Due to variations of individual sampler setups, no exact default depths are available,
although starting depths are provided in Table 3.1 on page 11. Instead, the process iscompleted by trial and error where the optimal probe positions are determined for each
vial.
Any of the Vial Type numbers can be assigned to any of the vials available (2 mL,
4 mL, 8 mL, and 12 mL). Follow the steps below to configure the vial depths for each
Vial Type.
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Configuration
WinFLOW Software Operators Manual: Chapter 3 11
Rev. 2.3
1. Click Vial Depthsin the RA Configuration dialog box. The Vial Depths dialog box
appears (see Figure 3.3).
Figure 3.4. Vial Depths dialog box
2. Enter the appropriate vial depths (in millimeters) in the corresponding boxes. Ifusing a static wash reservoir (no TWR), the range depends on the setup probe
position in the autosampler arm and the wash reservoir type being used; the range is
generally larger than 95 mm.
To get the maximum probe depth into the vial, set the probe at its uppermost posi-
tion and adjust the needle so that the tip of the needle barely clears the lip of the
wash reservoir. Lock the needle into position with a set screw.
Figure 3.5. Probe needle in relation to the wash reservoir
a. Determine which vial size will be used the most (from 2 mL, 4 mL, 8 mL, and
12 mL). This value normally designates Vial Type 1.
b. Verify Vial Type 1is set as the Default Vial Type in the Method Editor (see
Chapter 5, Flow Control Settings Screen on page 38).
Table 3.1. Vial Depth starting values
Vial Size
(mL)Rack Size
Approx imate
Starting Value (mm)
2 90-position 65
4 60-position with shelf 75
8 90-position 100
12 60-position 100
Needle
WashReservoir
Just clear the
reservoir
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Configuration
WinFLOW Software Operators Manual: Chapter 3 12
Rev. 2.3
c. Open the WinFLOW software and create a Sample Table with a cup position
adjacent to the position that contains a vial of Vial Type 1.
d. Send the sampler to the position chosen in step 1 by starting a run. Check the
depth of the probe tip to determine whether to raise, lower, or leave the tip.
NOTE: For 8-mL and 12-mL tubes:If the probe goes over the vial, but doesn't godown, the depth is set deeper than the sampler can go. Decrease the depth
until the probe will lower. This value is the maximum depth for any vial; the
maximum possible depth is 100mm.
e. If the depth needs adjustment, close WinFLOW and open Configure Winflow.
Make the appropriate depth changes and click OK.
f. Open Winflow and repeat steps dthrough euntil the optimal depth is reached.
g. Repeat steps athrough ffor each Vial Type.
NOTE: If only using one vial type, only set the depth for the default Vial Type.
NOTE: Vial types other than the default must be designated with their type number in
the Vialcolumn of the Sample Table for each non-default vial position.
Dilutor Setup If using the Autodilutor with the RA Sampler, click Dilutor Setup in the RAConfiguration dialog box. The Dilutor Setup dialog box appears (see Figure 3.6).
Figure 3.6. Dilutor Setup dialog box
By selecting Use Dilutor, Syringe Size and Syringe Speed settings become available
for input.
Use the pull-down menu to select the syringe size. The default syringe size is 2,500 L.
The available syringe sizes include the following: 1,000 L, 2,500 L, 5,000 L, and
10,000 L.
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Configuration
WinFLOW Software Operators Manual: Chapter 3 13
Rev. 2.3
Syringe size and the application determine proper syringe speed. The speed ranges from
25 for larger syringes to 50 for smaller syringes. The default speed values for each
syringe size are shown in Table 3.2.
Wash and Diluent OI Analytical offers two RA Sampler probe arm configurations. Specify which type touse by clicking Wash and Diluentin the RA Configuration dialog box. The Wash and
Diluent dialog box appears (Figure 3.7).
Figure 3.7. Wash and Diluent dialog box
1. Choose a Wash Source:
TWR(traveling wash reservoir)
Reservoir(fixed reservoir)
Cup Number(any specified cup location)
Most applications recommend using the TWR as the wash source. This configura-
tion allows a two-stage rinsing process.
Use the fixed reservoir as the recommended alternative to the TWR. Using a speci-
fied cup or vial location as the wash source is possible, but not recommended due to
volume limitations.
Table 3.2. Recommended Values for Syringe Sizes
Syringe Size(L)
RecommendedSpeed
Recommended CupSize
Rack Size forRecommended Cup
1,000 50 2-mL 90-position
2,500 35 4-mL 60-position with shelf
5,000 25 8-mL 90-position
10,000 22 12-mL 60-position
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Configuration
WinFLOW Software Operators Manual: Chapter 3 14
Rev. 2.3
2. Choose a Diluent Source:
Reservoir
Cup Number
3. Click OKto close the Wash and Diluent dialog box.
Rack Placement Setup the racks being used with the RA Sampler by clicking Rack Placement in theRA Configuration dialog box.The Rack Placement dialog box appears (Figure 3.8).
Figure 3.8. Rack Placement dialog box
The RA Samplers default configuration uses three sample racks and one standards
rack. Use up to six sample racks:
The preprogrammed sample rack sizes are 21, 24, 40, 60, 90, Incubtr2, and
Incubtr3.
The default sample rack size is 90.
The available standard rack sizes are 10 and 20.
The default standards rack size is 20.
Click OKto close the Rack Placement dialog box.
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Configuration
WinFLOW Software Operators Manual: Chapter 3 15
Rev. 2.3
Rack Editor The Rack Editor configures the RA Sampler to use virtually any size or type of rack.Program the rack size and type by clicking Custom Rack Editorfrom the RA
Configuration dialog box. The Rack Editor dialog box appears (Figure 3.9).
Figure 3.9. Rack Editor dialog box
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WinFLOW Software Operators Manual: Chapter 3 16
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To use racks other than those provided with the RA Sampler, measure the rack and vial
slot dimensions and the distance between each vial slot. Enter these measurements into
the corresponding locations in the Rack Editor. When saved, custom racks display as an
option in the Rack Placement screen. Create both sample and standard racks using the
following steps.
1. Click Add New Type. Type the custom rack name and click OK.
2. Select Add Cups. The Add Cups dialog box appears (see Figure 3.10).
Figure 3.10. Add Cups dialog box
3. Enter the following information in the corresponding fields:
4. Click OK. Click Close to exit the Rack Editor dialog box.
First Cup #: Number between 1 and 99
Cup Wid (mm): Cup width in millimeters
Number of Cups: Number of rows (left to right) and number of
columns (back to front)
First Cup Position (mm): Distance in millimeters from the standard
trays upper left corner (closest to the samplerbody) to the middle of the first cup
Inter-Cup Spacing (mm): Distance from the cup-to-cup center in
millimeters
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5. Click Rack Placementin the RA Sampler Configuration dialog box to access the
Rack Placement dialog box (Figure 3.7).
Figure 3.11. Rack Placement dialog box
4. Select the custom rack name just created for the appropriate rack placement. After
selecting a custom rack, the Align Rack dialog box appears as shown in Figure
3.12(if the dialog box does not appear, click the Alignbutton beside the custom
rack).
Figure 3.12. Align Rack dialog box
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6. Click OKto accept the default settings or enter new values to adjust the offset. The
RA Sampler rack is now ready and displays the custom rack as shown in Figure
3.13(if the custom rack screen does not appear, click OK to close the Rack
Placement dialog box and the Custom Rack Editor button).
Figure 3.13. Rack Editor dialog box displays a custom RA Sampler rack ready for use
Configuring the 3360 Sampler
1. Select 3360in the Configure WinFLOW dialog box.
2. Click Configure Sampler. The 3090/3360 Configuration dialog box appears(Figure 3.14).
Figure 3.14. 3090/3360 Configuration dialog box
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Vial Depths Adjust the depth that the sample probe travels into a sample cup.
1. Click Vial Depths. The Vial Depths dialog box appears (Figure 3.15).
Figure 3.15. Vial Depths dialog box
Enter the desired vial depths (in millimeters) in the corresponding boxes. The depths
correspond to the depth of the needle when used with the following cups:
Other settings may be used if other types of cups are used in the sampler. This
information is utilized in the sample table, to allow the operator to include multiple cup
types in a single run.
Table 3.3. Vial Depths
Cup TypeDefault Depth
(mm)
Cup Dimensions
(mm)
Cup Capacity
(mL)
1 150 13 x 100 8
2 93 12 x 25 2
3 113 12 x 30 4
4 150 16 x 100 12
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Dilutor Setup If using the Autodilutor with the 3360 Sampler, click Dilutor Setupin the 3090/3360Configuration dialog box. The Dilutor Setup dialog box appears (Figure 3.16).
Figure 3.16. Dilutor Setup dialog box
By selecting Use Dilutor, Syringe Size and Syringe Speed settings become available
for input.
Use the pull-down menu to select the syringe size. The default syringe size is 2,500 L.
Syringe size and the application determine proper syringe speed. Table 3.4shows the
recommended speeds for each syringe size, as well as the cup size that is typically
utilized for each syringe size.
Table 3.4. Recommended Values for Syringe Sizes
Syringe Size
(L)
Recommended
Speed
Recommended Cup
Size and Type
Rack Size for
Recommended Cup
2,500 23 4-mL type 38-mL type 1
12 mL type 4
60-position with shelf or90-position
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Wash and Diluent To provide an alternate source of wash or diluent solution, click the Wash and Diluentbutton. The Wash and Diluent dialog box appears (Figure 3.17).
Figure 3.17. Wash and Diluent dialog box
1. Choose Reservoirto draw wash/diluent from the rinse station.
2. Click Cup Numberand supply a cup number and cup type if an alternate solution
is needed.
Using a specified cup or vial location as the wash source is possible, but not recom-
mended due to volume limitations.
Rack Placement Setup the racks being used with the 3360 Sampler by clicking Rack Placementin the3090/3360 Configuration dialog box. The Rack Placement dialog box appears (Figure
3.18).
Figure 3.18. Rack Placement dialog box
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The 3360 Sampler uses four sample racks and one standards rack.
The preprogrammed sample rack sizes are 60 and 90.
The default sample rack size is 90.
The available standard rack sizes are 10 and 12.
The default standards rack size is 10.
Peck Probe duringRinse
Check this checkbox to rinse the sampler probe by pecking. Pecking is where the probe
is plunged into and out of the rinse position three times between samples. This rinse
method is typically used with SFA.
Leave this box unchecked in order to rinse the probe by simply lowering it into the rinse
position. This rinse method is typically used with FIA.
Configuring the 3090 Sampler
1. Select 3090 in the Configure WinFLOW dialog box.
2. Click Configure Sampler. The 3090/3360 Configuration dialog box appears
(Figure 3.19).
Figure 3.19. 3090/3360 Configuration dialog box
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Vial Depths Adjust the depth that the sample probe travels into a sample cup.
1. Click Vial Depthsin the 3090/3360 Configuration dialog box. The Vial Depths
dialog box appears (Figure 3.20).
Figure 3.20. Vial Depths dialog box
2. Enter the appropriate vial depths (in millimeters) in the corresponding boxes. The
vial depths correspond to the depth of the needle when used with the following
cups:
Other settings may be used if other types of cups are used in the sampler. This
information is utilized in the sample table to allow the operator to include multiple cup
types in a single run.
3. Click OKto close the Vial Depths dialog box.
Table 3.5. Vial Depths
Cup TypeDefault Depth
(mm)
Cup Dimensions
(mm)
Cup Capacity
(mL)
1 150 13 x 100 8
2 93 12 x 25 2
3 113 12 x 30 4
4 150 16 x 100 12
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Wash and Diluent To provide an alternate source of wash or diluent solution, click the Wash and Diluentbutton. The Wash and Diluent dialog box appears (Figure 3.21).
Figure 3.21. Wash and Diluent dialog box
1. Select a Wash Source:
a. Choose Reservoirto draw wash from the rinse station.
b. Click Cup Numberand supply a cup number and cup type if an alternate
solution is needed.
Using a specified cup or vial location as the wash source is possible, but not
recommended due to volume limitations.
2. Choose a Diluent Source.
a. Choose Reservoirto draw diluent from the rinse station.
b. Click Cup Numberand supply a cup number and cup type if an alternate
solution is needed.
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Rack Placement Setup the racks being used with the 3090 Sampler by clicking Rack Placementin the3090/3360 Configuration dialog box. The Rack Placement dialog box appears (Figure
3.22).
Figure 3.22. Rack Placement dialog box
The 3090 uses a single sample rack and one standards rack.
The supported sample rack sizes are 60 and 90.
The default sample rack size is 90.
The only supported standard rack sizes is a 10-position rack.
Peck Probe during
Rinse
Check this checkbox to rinse the sampler probe by pecking. Pecking is where the probe
is plunged into and out of the rinse position three times between samples. This rinsemethod is typically used with SFA.
Leave this box unchecked in order to rinse the probe by simply lowering it into the rinse
position. This rinse method is typically used with FIA.
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Ch a p t e r 4 Introduction to
WinFLOW
Starting WinFLOW
1. Double-click the WinFLOW icon or select WinFLOWfrom the Start menu.
2. Enter the serial number included in the WinFLOW documentation.
NOTE: WinFLOW only requests the serial number the first time the software starts.
3. If password protection is enabled for program startup, enter the user name and
password, and click OK. For more information on password protection see
System Security in this chapter.
NOTE: All instrument modules must be properly powered on and initialized for
online operation.
Select View DialogBox
WinFLOW displays the Select View dialog box on startup (Figure 4.1).
Figure 4.1. Select View dialog box
The Select View dialog box allows easy, single-click access to WinFLOWs four
primary options. It also provides an icon to exit WinFLOW. Table 4.1outlines
WinFLOWs four main options.
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When the Select View dialog box appears, the main menu bar displays File, Tools, and
Window menus, which can also be accessed from any WinFLOW screen. The File
menu also provides an Exit option.
Tools Menu
The Toolsmenu provides access to screens that establish WinFLOW preferences,
configure instruments, and set pump speeds, valve states, and heater temperatures.
Figure 4.2. Tools Menu Preferencesaccesses the Preferences dialog box.
Configurationaccesses the Configuration dialog box.
Auto Event Scheduleraccesses the Event Scheduler dialog box.
Instrument Controlaccesses the Instrument Control dialog box.
Pump Timerdisplays the number of hours the pumps are used.
Table 4.1. WinFLOW main options
Main Option Select ViewIcon
ShortcutKey
Small Icon Function
Collect Data F2 Initiates data collection following
sample table and method selection.
Sample Table F3 Opens a table displaying sample
cup number, name, type, replicate,
dilution, and weight factors.
Method Editor F4 Sets data collection parameters and
calibrant and QC information.
Data Analysis F5 Accesses results from any
previously saved run. Contains
peak plot, peak table, calibration
plot, and calibration data tables.
Exit F6 N/A Exits WinFLOW.
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Preferences Set global WinFLOW preferences such as default views and languages using thePreferences dialog box (Figure 4.3).
Figure 4.3. Preferences dialog box
Configuration The Configuration dialog box (Figure 4.4) provides information about the instrument
platform, such as the autosampler installed with the system, the firmware version, andthe number of communicating detectors (channels), valves, and pumps.
The Configuration dialog box allows the operator to determine if the analyzer, as well
as each installed component, communicates with the computer. Selecting Configure
when the analyzer or a component loses communication to re-establish a link between it
and the computer.
Security Sets WinFLOW to request a password on startup. For more
information see System Security on page 33in this
chapter.
Save Views Sets WinFLOW to display any screen on startup that
remained open when WinFLOW closed.
NOTE: Save Views does not bring up results and analysis files left open when
exiting.
Verify Close Sets WinFLOW to automatically prompt to verify theoperators intention to exit the program.
Re-enable Helpers Implements various warning screens that can be disabled
as operators gain experience with WinFLOW.
Language Select from English, French, German, or Spanish. Contact
your local OI Analytical distributor for more information
about other available languages.
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Figure 4.4. Configuration dialog box
Events Scheduler The Events Scheduler dialog box (Figure 4.5) sets what action(s) the pump(s) take(s)when any of the specified events occur, such as the end of an analysis.
Figure 4.5. Events Scheduler dialog box
Add Selects the event, time, and action.
Edit Edits any previously chosen event.
Remove Removes any previously chosen event.
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Instrument Control Manually set pump speeds, valve states, and heater temperatures from the InstrumentControl dialog box (Figure 4.6).
NOTE: The heater temperature settings do not control the Power Base cartridge
heaters.
Figure 4.6. Instrument Control dialog box
Pump Timer The Pump Timer dialog box (Figure 4.6) indicates the number of hours the pump(s)run. Use this feature to determine when consumables, such as pump tubes and
membranes, need replacing. Reset the pump timer at any time.
Figure 4.7. Pump Timer dialog box
Window Menu
The Windowmenu contains options for displaying open windows within WinFLOW.
The Window menu lists all open windows. Access any open window from the Window
menu by selecting the name or entering the number associated with the window.
Figure 4.8. Window menu Cascadeoverlaps open windows.
Tileplaces open windows side by side.
Arrange Iconsplaces minimized WinFLOW screens along the bottom the Select View
dialog box.
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About Menu
The Aboutmenu contains information about software and firmware versions, as well as
provides technical support access numbers.
Shortcut Keys
WinFLOW contains several keyboard shortcuts. These function keys provide an
additional method for locating and enabling various software activities. Table 4.2
describes the available function keys.
These function keys duplicate the Select Viewicons, which are also available from the
icon ribbon or the pulldown menu. Therefore, WinFLOW provides multiple avenues for
accessing the most frequently used software features.
Icon Ribbon
The following list details the various icons and their purposes.
Table 4.2. Shortcut keys
Function Key Description
F2 Opens the Data Collection dialog box.
F3 Opens the Sample Table dialog box.
F4 Opens the Method Editor dialog box.
F5 Opens the Data Analysis dialog box.
F12 Exits WinFLOW.
Opens the Data Collection screen.
Opens the Sample Table screen.
Opens the Method Editor screen.
Opens the Data Analysis screen.
Initiates data display.
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Resets to the baseline views beginning, which deletes all data collected.
Fast forward icon, which initiates data collection.
Pauses data collection.
Stops data collection.
Zeros the baseline signal on the peak plot.
Removes or restores the grid lines in the peak plot and calibration plot.
Peak plot update lock freezes the baseline update used during data
collection.
Activates the peak editor cross.
Deletes or reprocesses peaks.
Recalculates results.
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System Security
System security reduces unauthorized WinFLOW software access or use. It provides
simple, password-protected access to WinFLOW and some of its features. Accessing
the Security screen alwaysrequires a password.
If the system password is lost, contact OI Analytical Customer Support at(800) 336-1911 or (979) 690-1711.
1. Select Preferencesfrom the Toolsmenu to access the Preferences dialog box
(Figure 4.3). Select the Security checkbox. The password dialog box appears
(Figure 4.9).
Figure 4.9. Password dialog box.
2. Enter the user name and password. Click OKto return to the Preferences dialogbox.
NOTE: Use the default user name and password when first activating the Security
option. The default user name is systemand the default password is
manager. Neither entry is case-sensitive.
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3. Click Set Privilegesto edit, delete, or add a new user name and password. The
Security dialog box appears (Figure 4.10).
Figure 4.10. Security dialog box
4. Select Edit, Delete, or Add, and follow the instructions presented to edit, delete, or
add a user name and password.
WinFLOW Quick Start
NOTE: The following instructions assume all modules and hydraulic connections are
installed.
1. Turn on all Flow Solution modules and the computer.
2. Start the WinFLOW software.
3. Verify all tubing connections are made. Initiate pump(s) rotation by pressing the
pumps Runbutton.
4. Click the Method Editor icon. Verify the method is configured for the analysis to
be performed. Save any changes to the appropriate file.
5. Click the Sample Tableicon. Alter an existing table or create a new sample table.
Save any changes to the appropriate file.
6. Click the Data Collecticon. Select a method and sample table.
7. Enter an operator name, operator ID, and filename for the run results.
8. Click the Play icon and monitor the baseline. Once the baseline stabilizes with
startup solution, switch to reagents and again monitor the baseline until it stabilizes
(typically 1530 minutes).
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9. While the baseline stabilizes, load the autosampler trays according to the sample
table.
10. Click the Fast Forward icon when the baseline stabilizes.
11. Monitor the run until the SYNC peak elutes and is successfully marked. Allow the
run to continue.
If the operator completes the run or the run completes automatically, the data saves
automatically to the result file defined in step 7.
NOTE: Select the Stopicon to stop the run. Save results once the completed run
displays.
12. Review the results.
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Ch a p t e r 5 Method Editor
The WinFLOW Method Editor controls how WinFLOW collects and interprets data.
General Description
Open the Method Editor using one of the following ways:
Click the Method Editor icon from the Select View dialog box,
Click the Method Editor icon on the main icon ribbon, or
Press [F4].
Depending on the installed hardware, the Method Editor includes screens that modify
all method parameters necessary for running an analysis. Use the following six screenswith flow analysis hardware:
Flow Control Settings
Detector/Channel Settings
Signal Filter and Marking Settings
Calibration/Quantitation Settings
Timed Events Editor
Calibrants Table
This chapter discusses each of these screens and their associated menus. After setting
parameters properly, these screens do not require modifying during future analyses for
specific analytes.
Locate each screens title at the top of the window. The window bar also contains the
open methods name. Access each screen using one of the following ways:
Click the screens icon in the Method Editor icon ribbon,
Click the Method Editor icon ribbons advance or reverse arrow until the desired
screen appears, or
Go to the Method Editors Page menu, which lists each of the screens separatelyalong with their associated quick key functions.
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Method Editor IconRibbon
Opening a Method A method automatically opens when accessing the Method Editor. Open a previouslysaved method or an installed method using the following steps:
1. Go to theFilemenu and select Open.
2. Select the method to open, then click OK.
NOTE: If the methods platform is not the same as the current platform, a dialog box
appears warning that other method options need updating to match the
platform selected. If selecting Yes, WinFLOW adapts some method
parameters to allow WinFLOW to work on the current platform.
CAUTION:
Always use Save Aswhenaltering an existing
method so the original
does not become
overwritten.
3. After the method loads, modify it if necessary. See Creating a Method on page 38in this chapter.
Advances to the next screen.
Accesses the Flow Control Settings screen.
Accesses the Detector/Channel Settings screen.
Accesses the Signal Filter/Marking Settings screen.
Accesses the Calibration/Quantitation Settings screen.
Accesses the Timed Events Editor screen.
Accesses the Calibrants Table screen.
Returns to the previous screen.
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Method Editor
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Creating a Method The fastest way to create a method consists of altering an existing method and thenusing Save Asto save the new method under a new filename. WinFLOW installs with
the following six preset methods: single and dual methods for the Flow Solution 3000
(FS 3000) and the Flow Solution IV (FS IV), and a three-channel method for the FS IV.
Use these methods as templates for creating new methods. Most parameters in the
preset methods do not need changing when creating a new method for a given
chemistry.
Saving a Method When altering a method and the changes have not been previously saved, the Save Asdialog box automatically appears when another method opens.
NOTE: Saving a modified method as its original name overwrites the original settings
in that method. Save As modifies a method under a different name to maintain
the original settings.
Flow Control Settings ScreenSelect an instrument, activate channels, enable the baseline verification function, set the
sampling mode, set the default vial type (when using the Model 3090, Model 3360, or
RA Sampler), and define pump speeds from the Flow Control Settings screen
(Figure 5.1). Access the Flow Control Settings screen by clicking its icon from the
Method Editor icon ribbon, by going to the Pagemenu and selecting Flow Control, or
by pressing [Shift]+[F1].
Figure 5.1. Flow Control Settings screen
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Method Editor
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Instrument Specify one of three instrument platform groups:
FS III/IV/3100
FS 3000
Microbes-Coli
If the platform changes, a warning message displays stating that parameters need
updating to reflect the new platform.
NOTE: Changing the instrument selected does not automatically change the hardware
configuration. Although methods for any platform can be created at any time,
a method cannot be used unless WinFLOW is configured to function with the
current hardware (see Chapter 3, Configuration).
Sampling Mode Set Sampling Mode to one of two options:
Use Single Sample mode by performing the following steps:
1. Select Single Sample as the Sampling Mode in the Flow Control Settings screen.
2. Proceed with data collection as described in Chapter 7, Data Collection on
page 69.
At the point of data collection, the Single Sample Mode dialog box appears
(Figure 5.2).
Figure 5.2. Single Sample Mode dialog box
Uninterrupted Allows operation with an integrated autosampler. All samples in
a sample table analyze in a single, uninterrupted run.
Single Sample Allows flow injection analysis (FIA) operation with or without
an autosampler, or continuous flow analysis operation with an
autosampler. Each sample loads and injects when the operator
chooses.
Interval Currently unavailable.
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3. For FIA operation without an autosampler, manually place the sample needle into
the sample or standard solution and click Sample. The sample or standard aspirates
into the sample loop for the time specified in the timed events table (Figure 5.8)
(see Timed Events Editor Screen on page 52in this chapter). When the loop
valve rotates to the inject position, place the sample needle into the wash source.
For FIA or segmented flow analysis (SFA)-continuous flow operation with anautosampler, click Samplewhen the next sample standard is ready. The
autosampler automatically places the sample needle into the sample or standard vial
and extracts the sample or standard for the time specified in the timed events table
(Figure 5.8). The sample needle then returns to the wash source.
Figure 5.2displays after each samples cycle time completes until all the samples in
the sample table are processed.
The operator can also use a blank table each time a sample or standard aspirates.
1. Select Generate Blank Tablein the Run Setup dialog box, which appears when
data collection initiates (Figure 5.3).
Figure 5.3. Selecting the single sample blank table option
2. When the Single Sample Mode dialog box appears (Figure 5.2), click Edit. A blank
table appears.
3. Fill in the next sample or standard and close the window. WinFLOW prompts to
save the table. Saving the table automatically begins the analysis.
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CCV/Drift Fail Automatic recalibration options require deciding on a system action following a failedcontinuing calibration verification (CCV). For more information on CCVs, see
Continuing Calibration Verification on page 56in this chapter.
Vial Types Set Vial Types when using the Model 3090, Model 3360, or RA Samplers. WinFLOWcan use four operator-defined vial types within each analysis. Define each vial types
depth in the WinFLOW Configuration dialog box (see Chapter 3, Configuration on
page 7). Set the default vial type to represent the majority of the operator-defined vial
types used.
Off-scale (HI)Samples
Set autodilution options when using the Model 3360 or RA Samplers with the
Autodilutor. Enable autodilution by selecting Enable Autodilution. Once autodilution
enables, the following options become available:
Flag Only Places an F in the results tables flag column.
Pause Run Flags the calibrant and aborts the analysis.
Recalibrate Flags the calibrant and reruns the calibration section. Sampling
resumes at the cup after the last successful CCV.
Dilution Factors The Model 511 Autodilutor contains hardware-fixed
dilution factors.
The Autodilutor allows selecting a continuously variable
set of dilution factors. Enter up to five dilution factors,
each separated by commas. The Autodilutor uses the first
entered dilution factor for any sample that goes over thehighest calibration standard concentration. The autodilutor
uses subsequent operator-defined dilution factors if the
diluted sample concentration is still too high. Although the
autodilutor has a 1:24,000-step resolution, dilution factors
greater than 200 should not be used.
CCV Frequency Chooses the frequency of running CCVs with the diluted
samples (e.g., run a CCV every 10 samples). Designate the
CCV in the sample table after the dilution cups (see
Figure 6.1 on page 58and Table 6.2 on page 60).
Number of
Followers
Selects the number of followers to be run. A follower is the
sample(s) after an over-limit (HI) sample. Therecommended setting is two followers.
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Insert Baselines Insert wash baseline (WBL) points at regular intervals. A WBL point forces thebaseline through that point. When using the RA, Model 3090, and Model 3360
samplers in a segmented system, the WBL sample is taken from the wash reservoir
instead of a sample cup. For a FIA system, the sample probe stays in the wash, but the
valve does not inject, and carrier solution determines the baseline. Enable baseline
verification by selecting Enable under Insert Baselines, then set the desired First
position, Interval, and Duration.
When enabled, the baseline verification operates on all the activated channels. Baseline
verification points, which are designated WBL, do not appear in the sample table, but
they do appear in the sequence table preview.
Pump Control Control pump speed when operating the Precision Pump with the FS 3100, FS 3000,and CNSolution analyzers. Set these values in 1% increments.
Automated Precision Pump Control Opt ions
Refer to the method for the operating speed of the pump for the individual application.
Operate the Precision Pump at 40% speed in a counterclockwise direction
(40 setting) for most applications. Typically use a 10% slow speed (10 setting).
Automated FS 3000 and CNSolution 3000 Pump Control Opt ions
The FS 3000 and CNSolution 3000 pump typically operates at 100% full speed in aclockwise direction with the pump speed switch in the low position. A common slow
speed setting is 50%, which means the pump turns at full speed 50% of the time and
turns off 50% of the time. For a 30-second cycle time and a 50% slow speed setting, the
pump operates for 15 seconds and then stops for 15 seconds.
Start Position Provides the sample table location where the first baseline
verification point occurs. Check the sequence table preview in the
Data Collection screen to verify correct placement.
Interval Sets the number of samples between baseline verifications (250).
Duration Sets the number of baseline verifications to run consecutively (15).
Run Speed Sets the desired pump speed during normal operation.
Slow Speed Sets the desired pump speed used when the Pump Slow command
activates either manually or automatically. For manual operation,
see Chapter 4, Instrument Control on page 30. For automatic
operation, see Chapter 4, Events Scheduler on page 29.
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Method Editor
WinFLOW Software Operators Manual: Chapter 5 43
Rev. 2.3
Detector/Channel Settings Screen
The Detector/Channel Settings screen (Figure 5.4) sets parameters for each detector
channel individually. Update information for a particular channel by selecting the
channels line in the Channels list box. The information on the screen pertains only to
the highlighted channel. Only available channels appear in the Detector/Channel
Settings screen. Access the Detector/Channel Settings screen by clicking its icon fromthe Method Editor icon ribbon, by going to the Pagemenu and selectingDetector/
Channel, or by pressing [Shift]+[F2].
Figure 5.4. Detector/Channel Settings screen
Channels The Channelslistbox displays all the available channels. The number of availablechannels depends on the number of installed detectors. A channel presents as available
following successful communication with a detector.
Activate a channel by double-clicking the channel name. A number sign (#) sign
appears to the left of the activated channel name (Figure 5.4). Deactivate a channel by
double-clicking the channel name again. Method parameters only apply to active
channels. Therefore, channels must be activated before moving to other Method Editor
screens.
NOTE: If opening a method that designates an unavailable channel, an X appears
on the channel names left. Re-establish contact with the channel by going tothe Main menu and selecting Setup and Configure. If unsuccessful, the
systems exits and restarts WinFLOW.
Name Designate a nameto describe the chemistry being run. WinFLOW uses the channeldesignation (e.g., Channel 1) as the default name.
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Method Editor
WinFLOW Software Operators Manual: Chapter 5 44
Rev. 2.3
Methodology Designate a methodology that describe the specific chemistry being run.
CAS Number Enter the Chemical Abstract Service (CAS) number for the standard used in theselected channel, if applicable.
Detector Settings
Mode Sets the detector mode as Photometric, Amperometric, ISE
(ion-selective electrode), Fluorometric, or Matrix(seawater
matrix). Select Photometric for the Expanded Range(ER)
detector. Select Amperometric for the Amperometric detector
and for the analog-to-digital (A/D) converter. Use ISE for all ion-
selective probes such as pH or fluoride. Use Fluorometric with
the fluorometric plug-in detector.
Acquisition Rate Determines the data collection rate in Hz (or points per second).
Options include 1, 2, 3, 5, or 10 Hz. The 1-Hz default valueusually suffices. When running very low analyte levels,
increasing the acquisition rate can result in a sensitivity increase.
When operating multiple channels, set the acquisition rate to 1
Hz so the computer does not overload with data.
Cell Potential Set the cell potential in amperometric mode using the detectors
10-turn precision potentiometer. Setting cell potential via
software is a future option.
A-D Gain Sets the analog-to-digital gain for the sample and reference.
Options include 1, 2, 4, 8, 16, 32, 64, 128, or Auto. Set Sample to
Autofor all photometric detectors. The photometric detectorautomatically sets the optimum gain based on the amount of light
reaching the photodiode. Set the amperometric detector to a fixed
gain of 1.
Set Reference to Auto for all photometric detectors. The
photometric detector automatically sets the optimum gain based
on the amount of light reaching the photodiode. The
amperometric detector does not have a reference gain.
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Method Editor
WinFLOW Software Operators Manual: Chapter 5 45
Rev. 2.3
ChannelSubtractions
Subtract peak concentration, peak height, or area in one channel from peak
concentration, peak height, or area in another channel. For example, nitrate-plus-nitrite
analysis typically occurs on one channel and nitrite-only analysis on another. Use the
Channel Subtractions function for automatically calculating nitrate concentration by
subtracting nitrite concentration from nitrate-plus-nitrite concentration. Enable Channel
Subtractions on only one of the two channels because peak concentrations in one
channel subtract from peak concentrations in another channel. For example, channel 1peak concentrations (nitrite) subtract from channel 2 concentrations (nitrate-plus-
nitrite). Therefore, enable Channel Subtractions on channel 1 and not channel 2.
Subtracted data (nitrate) appears with other data in the peak results table.
NOTE: When using Channel Subtractions, OI Analytical recommends using
concentrations rather than peak height or area.
Subtracted From
Channel
Selects the channel from which the current channel subtracts.
In the nitrate-plus-nitrite example, the Subtract From Channel
is the nitrate-plus-nitrite channel (channel 2).
Subtraction Name Designates the subtracted datas name. For example, if nitrite
peak concentrations subtract from nitrate-plus-nitrite peak
concentrations, name the subtracted data Nitrate.
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Method Editor
WinFLOW Software Operators Manual: Chapter 5 46
Rev. 2.3
Signal Filter and Marking Settings Screen
Set smoothing, baseline marking, and peak marking options for each channel
individually from the Signal Filter and Marking Settings screen (Figure 5.5).
Access the Signal Filter and Marking Settings screen by clicking its icon from the
Method Editor icon ribbon, by going to the Pagemenu and selecting Signal Filter/Peak Marking, or by pressing [Shift]+[F3].
Figure 5.5. Signal Filter and Marking Settings screen
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Method Editor
WinFLOW Software Operators Manual: Chapter 5 47
Rev. 2.3
Smoothing Select one of three software-based signal processing algorithms to reduce noise,improve precision, and ease integrating difficult peaks (small size relative to noise).
Choose None, Moving Average, Olympic, or Savitzky-Golay. Select one of these
algorithms in advance. If selecting None, access these algorithms after the run
completes and apply them in the results file.
Moving Average Selects a simple algorithm for filtering where the average of an
input set is used to generate one output value. This algorithm
tends to reduce peak height maxima due to the lower values on
either side of the peak. Peak area measurements remain largely
unaffected. Use a maximum of nine data points and one
iteration for best results with this algorithm.
Olympic Selects a variation of the moving average algorithm. It
eliminates high and low values and averages the remaining
values. This algorithm very effectively screens out spikes (high
frequency noise). Like moving average, the olympic algorithm
tends to reduce peak height. Peak area measurements remain
largely unaffected. Use seven data points (11 maximum) and
one iteration for best results with this algorithm when acquiring
data at 1 Hz.
Savitzky-Golay Selects an algorithm that multiplies each point by a weighting
factor and then averages the points in a data set. The factors,
derived from second- and third-degree polynomials, weight the
center of the data point set and progressively lessen the
weighting to the points further out. Unlike the other algorithms,
this setting minimizes peak height maxima distortions. Peak
area measurements also remain unaffected. Use several data
points and multiple iterations for best results with this
algorithm when acquiring data at 1 Hz.
Points Defines the number of input data points processed to generate
each output data point. Options include 3, 5, 7, 9, 11, 13, and
15. The data acquisition rate is a very important parameter in
determining the optimum number of points for a given peak
width. The default setting uses five data points, which is
adequate for most applications. Use a higher value when
processing extremely wide peaks.
Iterations Sets the repetition number that the algorithm treats the output
of one operation (filtering algorithm) as the input for the next
operation. It increases the degree of filtering (smoothing) whilekeeping the filter width (points) optimized for the peak width.
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Method Editor
WinFLOW Software Operators Manual: Chapter 5 48
Rev. 2.3
Baseline Marking Baseline Markingconsists of the following three parameters:
InvertedChemistry
Select Inverted Chemistry when the chemistry being run measures a decrease in
response instead of an increase. Select this option only if the analytical method for the
chemistry used states that it is an inverse chemistry.
NOTE: This feature can only be used with certain detector types.
Peak Marking
Lead Time Sets the time in seconds before the SYNC peak, which is used
as the initial baseline point. WinFLOW determines the
baseline lead after marking the SYNC peak by counting
backward from the SYNC peak marker. Be sure to set the
baseline lead time longer than half the SYNC peaks width so
WinFLOW does not mark the baseline on the rising slope of
the SYNC peak itself. As a general rule, set the baseline lead
time to 15 seconds more than the peak width. The def