V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

32
/3=F._ QLIKNJMR =F_ B;<Y[\WXZ\V)-ST O1U(P1U"A5* /398_ H:]CE?/30^ /3$D_ 20162018]2830^ 624G_ 75,462 20193#7! +/37@>% +,'&

Transcript of V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

Page 1: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

/3=F. _ QLIKNJMR=F�_ B���;<Y[\WXZ\V)-ST

O1U��(P��1U"A�5*/3�98_ �H:�]�C����E?�/30^/3� $D_ 2016���2018��]��28���30��^62�4G_ 75,462�

2019�3#7!+�/37��@>�%���+��,�'&

Page 2: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

• 76�0%��]Hge�J-<– 1973� nkolo-<

– 1992� 76�0%-<

• 1992� ;^��4– 76�0%��^�$[\eE@\4#

� ��F7�/6;^�* ��jomiocd

• 2013�]&18\)2– ":�89A^��h�e"^Q�^D�– � ��F7�/6;_,?�.20�Z

90;Q(�TfYRXQK2020�`Z]300;h+B(�Ve (I�3C)

– >�^!�p�Z_5���^'"aUS_500����^>GK4�Z_1���^>G

=5^PWf^NeF7�/6^;^��]HVe4#KOgbeL;^��4M

• 2017�)2^��3C– 2030�`Z]K� ��F7�/6;h

700;(�Ve

Page 3: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

��$ ����������#�� �"������!

0

20

40

60

80

100

120

140

0

5

10

15

20

25

30

35

40

45

50

1994 1996 1998 2000 2002 2004 2006 2008 2010 2012 2014 2016 2018

Nofac

cumulated

species

��������� �����������������

� #���

�����

�#����#���

��

��

#�

Page 4: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

��,*+-��,*+.�:��4D5�� %�����(�����)

2018���Arisaema abei a�Nc�g�W�H

Arisaema aprile Jf�Sc�g�W�H

Arisaema cucullatum t�c�g�W�H

Arisaema inaense Ggm�kc�g�W�H

Arisaema ishizuchiense ssp. Ishizuchiense GWb_c�g�W�H

Arisaema kuratae FvNc�g�W�H

Arisaema nagiense gNm�kc�g�W�H

Arisaema ogatae JL]c�g�W�H�aOWc�g�W�H�

Arisaema seppikoense [`oSc�g�W�H

Asarum hexalobum var. controversum WWMK�FJG�WXMK�FJG�

Asarum kinoshitae X}�HK�FJG

Asarum monodoriflorum zjf�K�FJG

Asarum sakawanum var. stellatum tWVMK�FJG

Asarum satsumense UavFJG

Asarum yaeyamense |I|vK�FJG

Asplenium tenerum JeyW^

Deparia minamitanii m}HLWQW^

Diplazium pin-faense pO�NW^

Diplazium subtripinnatum xh�wf�W^

Crepidiastrum ameristophyllum ~Z�k�^�

Saussurea mikurasimensis wO�XveHm��

Saussurea yakusimensis |OWvmT]G�|OWveHm���

Draba igarashii W�sWgZg

Ctenitis microlepigera SM�zHGjd

Dryopteris hangchowensis M�WvG�rT

Polystichum piceopaleaceum UO�XvGjd

Claoxylon centinarium [Mz�jM

Hypodematium fordii �}HM}HM�zH��n

Myrsine okabeana v�l]Gw�]_lg

Gastrodia albida |OWv|aW���

Gastrodia uraiensis ]qL�|aW���

Odontochilus hatusimanus kaWv��

Platanthera boninensis Wva�UN\H

Drynaria roosii kKvH�uW

Leptochilus decurrens JMjO�k��

Potamogeton praelongus gLlInz

Callianthemum kirigishiense M�NW\H

Deutzia naseana var. amanoi JMg�myHaN

Lycianthes boninensis xh�tJZM

Stachyurus macrocarpus var. macrocarpus gLlMqWStachyurus macrocarpus var. prunifolius kVO�MqW

Grewia rhombifolia mWlHJe�N�FalHJe�N�

Sciaphila yakushimensis |OWv\H

Procris boninensis [Mz�H�G\H

2017���Arisaema heterocephalum ssp. Okinawense JMg�c�g�W�H

Rhamnus kanagusukui myO�HyzfM

Pothos chinensis ~ZjkKZ�

Asarum caudigerum JgLUGW�

Asarum okinawense mgK�FJG

Asplenium oligophlebium var. iezimaense GIXv_{[�W^

Hymenasplenium subnormal HYG�tHnW^

Crepidiastrum lanceolatum var. daitoense ^GeH�^�

Cyrtomium macrophyllum var. microindusium Ov|q\ca

Chamaesyce sparrmannii u�XjhWM\H

Tripterospermum distylum kg|va���fH

Uraria picta t\lpXuPU

Vigna vexillata var. vexillata UO|FKUUR

Chionographis koidzumiana var. kurokamiana O�KwW�Ge\H

Ryssopterys timoriensis UUMKZ�

Goodyera fumata |qw�HL��

Habenaria stenopetala caJUN\H

Hetaeria oblongifolia JJKR�H��

Odontochilus tashiroi JJNw��

Platanthera okuboi k_X�Ha�UN

Polygala longifolia �}HM}HmykN

Persicaria attenuata ssp. Pulchra F�R]d

Persicaria japonica var. taitoinsularis ^GeHUO�]d

Elatostema yonakuniense �gOheMtS�

2016���Rhaphidophora kortharthii UMWvkqKZ�

Rhaphidophora liukiuensis mykqKZ�

Asplenium formosae vMjW^

Asplenium griffithianum pUUX��

Athyrium yakusimense |OWv]hGi��n

Cornopteris banajaoensis t\lWQ_W^

Diplazium kawakamii FJGL��n

Microlepia obtusiloba var. angustata t\lSHW}�W^

Gentiana yakushimensis |OWv��fH

Lycopodium fargesii mzYN��

Anoectochilus formosanus MlgW}Y��

Anoectochilus koshunensis SHW}�W}Y��

Cryptostylis arachnites JJYZxW��

Hancockia uniflora myO�\��

Macodes petola g�l�Kzy��

Phaius mishmensis myKO��

Thrixspermum fantasticum kLO�gLw��

Deutzia yaeyamensis |I|vmyHaN

Tectaria fauriei Sz_gglQW^

Tectaria kusukusensis gLlHYlW^

Thelypteris gracilescens Wv|��W^

Patrinia triloba var. kozushimensis WvM��GK

2015���Aeschynanthus acuminatus gLwKZ�

Lycopodium salvinioides my�H�Oml

Cryptostylis taiwaniana ]KJJJYZxW��

Platanthera stenoglossa ssp. Iriomotensis G�Jzce�u\H

Vrydagzynea nuda w\uW��

Plagiogyria koidzumii �}HM}HMXjJ

/'BD5��#����0�"0�&�;@809;A1:�;� �E��73C;2�

/�� %�����=;��7$�:� �E>@DC;2�

/���!<�?6���� %��������700�

Page 5: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

1990��~ 1,000��

2000��~ 50,000��

88��1,000,000,000��~

,�M 0���-.>37AGJKCEIK

100� 10,000 ~ 100,000�

10,000,000���

('M�'>�2?0���B&$�"��>#49 %'4;��'=�:=&$�*?!+

��?0����6@�)0��

��?0����)L/�'0��DFH��

��?� <-.<5ADNA��1��

Page 6: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

¥¬©µ¯1 ��s���'´�´z

�w=:��_W��(ux (RNA-seq)�¤ª°mhux (RAD-seq) �j�uG

ae�¥¬©µ¯2 T�o%F�'´�´Y�/-c�p25�u?

¥¬©µ¯3 N]�AgM'´q0��+�§³¨µ

�kS�7c� n¥¢¦&~

¥¬©µ¯4 �.�F�'´�´z�¤ª°6#mhuxL��_�4\

�¤ª°²®±��

f��(16#¡���kS�7c�|�¡3�

����-�¥³­±��¤ª°6#¡x����K) ¡¤ª°6#¡���£±µ«³£��

B}�����(ESU)¡I�� y��^E�V*���`9�J|��3�W DE��Q

<

3O

l8

kS

RNA-seq'���6#

RAD-seq'���6#

uGiHuGiH�j�

uGiH uGiH

�RNA-seq6#��_W��(¡uG�>��v�"R�bf�C��[U��

�C,��(�rd�;*↑ <�-��c��rd����

�{�´}4o�v�↑!XZ$&�@���t

←@��P 

Page 7: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

�-/FHJ�IKB�E�!

>NTP\V1? ��.=:4�;.GMSW��79

>NTP\V2? ��.G6��G8�→MSW��F�CA�BE8�%→��(G�&F�CA!,��

>NTP\V3? ��.G��5�G�$→���G$�D�3�G�&[8�

>NTP\V4? �<[��NZUYL*@B��.G2�MSW79

→"'��G+ D��XROQG7�

>NTP\V1? HJ!,[ ),E��0G#1

Page 8: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

-, 1 0

N

9A 6 (((

6 ) (

9A 6 )

A e ( )

R

2( ) (

)

2

4 6 ( (

e 6 ( )

8 A ( () (

A 2 (

6

6 )

6

9 A 6 (

A 9 A 6

88 6 (

e

7 e (( )(

5 e )(

8 e ( )

5 A

2q

N A2q

) ()

6 5

6 ( )

R 5 (

(

( ((

) )

3 s

9 ) )

,- 1 0

A

3e 3

3s

6 (

8 6 (

R 3e

( 3 ) )

6

8 6 )

A 8 6 ( )

77 6 ()

5

3 3

q 3 3

(

q 3 3

A 6 q 3 q )

5 ( (

7 D ) (( 3 ( (

D ( ( 3 ( (

4 2

) ( ( (

9 )

A 8 6 ( )

9 8 )

������������"����

� �#!1$RNA-seq�������� ���

→� �#!2�

� �#!1$RAD-seq�����"������

→� �#!3�

Page 9: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

�-/FHJ�IKB�E�!

>NTP\V1? ��.=:4�;.GMSW��79

>NTP\V2? ��.G6��G8�→MSW��F�CA�BE8�%→��(G�&F�CA!,��

>NTP\V3? ��.G��5�G�$→���G$�D�3�G�&[8�

>NTP\V4? �<[��NZUYL*@B��.G2�MSW79

→"'��G+ D��XROQG7�

>NTP\V1? HJ!,[ ),E��0G#1

Page 10: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

RNA-seq��OZ��I �\@�11

L:!U�JMK2QUW��XI �P/V[

K9SVNXY#B1V<��TYa

�6�=2

���G.%+2

)0W�'R[2

]^_�X"�E��H

�� ����

Makino & Kawata (2012)

(R2 = 0.86)

������

7�

&�

��

"��/

�3V�/

�/X"��@� (PROVEAN)

K0,E��X581?A (RNA-seq)

E��F>H`-�D�;

��2X<��X@�: 4*��2X(C]^_?$

Page 11: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

12

� �� ��: ����� ��!"#��

!"#� ������ ������$������

Page 12: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

13

�5�0��2

D��E=K�PL/�C 9�K�J

/�C 9�

H�GP�5�0��2RI'�D��Q<3K�LISNI/�C 9�T�JH�4D���X>)MV�(QD��1$,OR�UWPJ��K�%H#���Q;�-J6*

A8&B2

'�D��<3F

'���Q<3K�L:�

��2Q:�!Q?�: 7. "2Q+@YZ[>)

Page 13: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

�-/FHJ�IKB�E�!

>NTP\V1? ��.=:4�;.GMSW��79

>NTP\V2? ��.G6��G8�→MSW��F�CA�BE8�%→��(G�&F�CA!,��

>NTP\V3? ��.G��5�G�$→���G$�D�3�G�&[8�

>NTP\V4? �<[��NZUYL*@B��.G2�MSW79

→"'��G+ D��XROQG7�

>NTP\V1? HJ!,[ ),E��0G#1

Page 14: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

��*G��,��G�#

8QXY��?J��*D0+*G��,��L$/.!

TRPOWRVN���2(�"&*OWRVN

'�?JG�3 (x 1000�)

� 4�SMU

(x 1

000)

<)�=��*H>C?J:��;DEBAG?Z� F(-@K:��*;G#�9���HZ

8�*DI655���F4�%�

8OWRVNH75���F��%�

1�'� 1�'�

Page 15: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

����D6�)48SZ\X]_O#B8[VY^W_M-PL(1) F�N����J>I(2) $F�TRU(Ne)J+9M�KIF�JHQ

.;%��720

.;%�A��

�<���*&

Ne: 46900 Ne: 2820 Ne: 9110

8480��

13300��

#B8[VY^W_

�� G(

��'?�

��1�/

3�!�

Ne: 19900 Ne: 65 Ne: 573 Ne: 2130

19��

854��

1510��

"E@

5�

C

��8SZ\X]_

(2) ��8N��=��N�,

�:�.;

Page 16: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

uowmktt pjrinv

0.00

0.05

0.10

0.15

lqs��/$

0.00

0.05

0.10

0.15

lqs��/$

�B�3GF�3M2

1�`d(9a��lqs��/$y�XK*Lc�?W����

1�7`��@Alqs��/$y�<#'+b�&[,H\^&��cf

!�.5z� �#dz

x=d��c��&d�aX`XY4_�J]h[W6D$g��d8ah

�=c��C�%c�0

UVT���� ;:>ISNE-eZWQOPR"PP)

Page 17: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

�-/FHJ�IKB�E�!

>NTP\V1? ��.=:4�;.GMSW��79

>NTP\V2? ��.G6��G8�→MSW��F�CA�BE8�%→��(G�&F�CA!,��

>NTP\V3? ��.G��5�G�$→���G$�D�3�G�&[8�

>NTP\V4? �<[��NZUYL*@B��.G2�MSW79

→"'��G+ D��XROQG7�

>NTP\V1? HJ!,[ ),E��0G#1

Page 18: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

%��/79; !/1$>"-2/��.DNA)��%���,1&*03(.DNA) 45.'�2'

��8=:< ���0�$����8=:<

�$>��8=:<6�'+���0#� �

�$8=:<���0���� ����

Page 19: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

$057�,��"�!�,��/��).$(*2968+-��+'.$�#,2968+-��+'.$�134

P5 flow cell P7 flow cellRead 1 sequence primer

Read 1 Read 2 Index read

Read 2/Indexsequence primer

358

12 2 2 12 329 33 34 6 2565

Index

Indexed reverse primer

Common forward primer

1st PCR product

Forward primer

Reverse primer

Genome DNA

AnchorAnchor for 2nd PCR primer

SSR SSR

1st PCR:Multiplexed PCRwith tailed ISSR primers

2nd PCR:Tailed PCR with commonand indexed primers

Sequence of MIG-seq library

Highly reduced represetation library

Index-tagged library

Genome DNA

Pool, size select,quantify and sequence

SSR region

AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTG[SSR][anchor]NNNN...

CAAGCAGAAGACGGCATACGAGATXXXXXXGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGAC[SSR][anchor]NNNN...

P5 flow cell

anealing site of Read 2 sequence primer

anealing site of index sequence primer

anealing site of Read 1 sequence primer

P7 flow cell index

a

b

c

d

$MIG-seq,� ;��#,��%���,��<

�#:2968/�&*���, ���MIG-seq (Suyama & Matsuki 2015)

Page 20: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

,HPD@N9��<)8-?FK! $%9��*-���<#20 �R�134

,59;/6��7:&�"

BamboosPhoto by YS

CallitrisPhoto by Shota Sakaguchi

OaksPhoto by YS

SprucePhoto by YS

CycadsPhoto by José Said Gutiérrez-Ortega

LasianthusPhoto by YS

MangrovesPhoto by Gustavo Maruyama Mori

CypripediumPhoto by Keiko Kitamura

MachilusPhoto by YS

OxeraPhoto by YS

RhododendronPhoto by Yoichi Watanabe

˙HydrobryumPhoto by YS

SolidagoPhoto by Shota Sakaguchi

Pollen (HemerocallisTPhoto by YS

0.000

0.001

0.002

>MS+

EI@

OGQ+

JBQ@

A=+

EL@

CI+

��� ���

$%��*

(SN

P�/$���(�

)�*R��AQHN<�.4���9'�$�?FK��! $%�MIG-seq9��

Page 21: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

,=8���

VbWZ\T5-�!IAB(CR)

�Rc`[ �

V]^X\T����?0�(.4

_^U\T5-�!IAB(CR)

,=+�2RaS�

V]^X\T�34JCFPK��

�9*7DM#�EPG3%'�"HLO34I<J�A

����Jf

→MIG-seqHLN4<

�@e�Yd`cQ1CG��4I>�:&�B6I/�e����;�e)$I��

Page 22: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

-3

-2

-1

0

1

2

3

4

5

-6 -4 -2 0 2 4 6 8

GF>D=

?I@CD=

?EFAD=

��$��

?EFAD=�3&254BKHJ<MIG-seq69:��1;32527SNP�<85�� ��→M&<�%6,N"(�����5247��<+� )

?I@CD=

?EFAD=

�����

�.L�BKHJ<#03��&7-�*��/'7!�L����+�L��7���

Page 23: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

5(�? 2

"C�R

"C�P

"C�Q

�?

�?F

�?C�F �?C�2 �?C�":/9C�

P Q R

�@n��dmhka.TW��3\>�;&4-��\+�n���<�

lgmbfijec]1!�\�?Z�?Y>�0[ �→2\����

LKJDOMN,\A/n 8n�.[�VX#%)'��=�=o B���SGEFI�H$)'>�63��=�=o B���SGEFI�FF$

�?Y/9U`W"C�Q]>�0[]�?C�o�[^_2*C�\�7�→���<�

Page 24: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

�-/FHJ�IKB�E�!

>NTP\V1? ��.=:4�;.GMSW��79

>NTP\V2? ��.G6��G8�→MSW��F�CA�BE8�%→��(G�&F�CA!,��

>NTP\V3? ��.G��5�G�$→���G$�D�3�G�&[8�

>NTP\V4? �<[��NZUYL*@B��.G2�MSW79

→"'��G+ D��XROQG7�

>NTP\V1? HJ!,[ ),E��0G#1

Page 25: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

��� N

1�%���H

EHJ?���#�0�,

�9=-��

���5�4@<1�%���A34

���N0BC�=4?5��&

N0

���2$��!&

.*�/&

BC��%N�"%=��(?�)

�' >$+8D���2$��!&

���5�=4:7;=6EHJ?���5�4#�0�IGMFKL5�4

���5�4EHJ?���534#�0�IGMFKL534

Page 26: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

��� N

1�%���H

CFH=���#�0�+

�:<-��

���8�7>;1�%���?47

���N0@A�<7=8��&

N0

@A�%L�"%<��(=�)

���2$��!&

��1��,'38�<95#�0�+=47&?6B

.*�/&

���8�7CFH=���847#�0�GEKDIJ847

Page 27: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

*"�� �!2%���%#�0����(�/�'�,�3��$)4-� 1�������5� 1��

)

(

)

(

-

�(��.�&�+�

/2020�=7:300'��4CB ���.%�"';#�<�,7<91/��#�DEFG��D?8:-�:+�5B3870@A�$&H�&9��2�*

/���;�D�>� �*

���

�69+��!

@A�&H�$&9��(;�)

Page 28: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

*"�� �!2%���%#�0����(�/�'�,�3��$)4-� 1�������5� 1��

)

(

)

(

-

�(��.�&�+�

.����,!���#7�68����6*1-&90<

.����/�����7�(6214+'5��

���

�35)���

:;�"= "5��$7�%

Page 29: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

RCOP10�6mqlnotCOP10L��a]_eE�G?dD.– V4$12W 2020�h`dS=*��:e=*p)�ZN%\kS,d)�]_Yj:d�^j��-'e>�p�ZI�\kj

– V4$19W 2020�h`du0+�#�cbdMF^j6AS9�3�8�f�@Z�\ku� J1\kj

RT<(C�Ha�Oe�!�K2018rP��KsUdX[ik_YjQ0+�#�e��dM^j7;Q

R0+�#����22012-2020R/ 5�B�&�"

/ ?�geD.

30

Page 30: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

7RS-a�Ub7RS-c1) Makino T, Rubin CJ, Carneiro M, Axelsson E, Andersson L, Webster MT (2018) Elevated Proportions of Deleterious

Genetic Variation in Domestic Animals and Plants, Genome Biology and Evolution 10:276-290.2) Suzuki S, Sugai K, Uchiyama K, Katoh S, Kato H, Narita S, Isagi Y (2018) Development of microsatellite markers for

Callicarpa subpubescens (Lamiaceae), an endemic species of the Bonin Islands. Journal of Forest Research 23: 393-397.

3) Arima K, Kyogoku D, Nakahama N, Suetsugu K, Ohtani M, Ishii C, Terauchi H, Terauchi Y, Isagi Y (2019) Unilateral outcrossing between sexual morphs in a distylous primrose provides a novel hypothesis for the maintenance of heterostyly by overdominance. Evolutionary Ecology 33: 55-69.

4) Makino T, Kawata M, Invasive invertebrates associated with highly duplicated gene content. Molecular Ecology (� �).

5) Mizusawa L, Ishikawa N, Yano O, Fujii S, Isagi Y, Geographic distribution of ploidy levels and chloroplast haplotypes in Japanese Clerodendrum trichotomum s.lat. (Lamiaceae). Acta Phytotaxonomica et Geobotanica (� �).

6) Kurita K, Kyogoku D, Abe A, Yokota M, Isagi Y, Development of microsatellite markers for an endangered fern in the Ryukyus, Plagiogyria koidzumii (Palgiogyriaceae). Genes & Genetic Systems (� �).

7) Kishikawa K, Suetsugu K, Kyogoku D, Ogaki K, Iga D, Shutoh K, Isagi Y, Kaneko S, Development of microsatellite markers for complete cleistogamous species Gastrodia takeshimensis (Orchidaceae), with transferability for its chasmogamous sister G. nipponica. Genes & Genetic Systems (� �).

8) N6D�y8C�Ky�`P�y;C0�y^[J+yW�V,y�*.�ZyX%E�y\A@�y+�1y&>�Oy<4�=y]�L�y $�a�'tvxqkdfm(":Bn!TighpsuworjY�Q5zDNA�� (� �)z

b�Uc1) /496��U�U{_#��a2018)332) DNA����GFIU{N6D�lea2018)1133) 96Y�MH��U�U{_#��a2018)1134) /496����U: ?C�2a2019)33

Page 31: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

2�ćċ AŌ`ºHÂ1) ��b{ï�ض<ðÒ�?×¼ĊĔė��ċÎ!ćÑXñŎ2016Q6m18iïvo�3BÞvoåwï´Ä²¨30.ŏ2) ġōĽŋģŃŋĺī2016ï�ç»+ðÒ�?ĚÃĎąyuċ��=}YĚĐēėñŎ2016Q8m8iïÕ>AËAÓï¿´²100.ŏ3) ��¯~f§©5�ŊōĤĩńİĽï��-jð��Ċâč£ą! phylogeographers! ősĜĪĜĊõúėz�§©5�A��¢ċæ

�ćÝ�őñŎ2016Q10m29iïÕ>A�AÓĬŁĶōĹĞīï(�²¨120.ŏ4)P]28QSÕ>Ayu A�ÚÄTï�ç»+ðÒ�ÁtĆĐēėyuċ��=}YñŎ2016Q11m15iï¿´²60.ŏ5) 57ċ AÄTð�)ıņōĥĜŊōĤĩńİĽñïßK��ð57ĊĔėÒ�?ċÐòĚ�öüÿz�ċ×½YñŎ2016Q11m19iï

´Ä²¨20.ŏ6) 150ðöĕĀćûûĘċĨĝĠŋīĢļğñŎNPO�öĕĀćûûĘċ�¾;��ŏï��-jðČyćĈóÛęĂąøÿċö―��=

}Yöĕđÿyćċ�*―ñŎ2016Q11m30iïsÕe#đċĖcaféï(�²¨30.ŏ7) ClØNňİĵijōĮJîî·�ÄTêï��-jð�øēċÿā÷�ěĆøÿČėöĉėÏĚcėő��§©5�ćòó��ő z�­ñ

ŎêèéìQëméíiïClØNÇ Ô4%�ãï(�²¨50.ŏ8) ĢİħĭĞ$Æ�¬�ï�ç»+ð«®�Ò�ÁtĊ8Ąù¡ċ�@�HÈ¡ċ��ñŎ2017Q4m9iïx�Nx�·�ÀFċyï¿

´²30.ŏ9) ĠļĠł�)forever green project�)"KÊ�Gċy�äıĜōÄ��ïßK��ðyċ/ċ=}YĚ���Ċ�ýñŎ2017Q9m

24iï´Ä²¨80.ŏ10ŏ¤580Ø|ŀİį�U�ï�ç»+ðyu�\§ć��=}YĊăòąAďóñ Ŏ2017Q11m19iï>ÜN£¬,��A°ĬŋĮōï

´Ä²25.ŏ11) ��b{ï�ض<ðÒ�?×¼ĊĔė��ċÎ!ñŎ2017Q12m14iïE6��)¤�å¥Awï´Ä²¨60.ŏ12) �¸�ÚĩŋĿĪĞłŎprċkĕüh̵Ē�ċ�ÍĩŋĿĪĞłin"�ŏïßK��ð��ċ57YĚ�öüÿÇöĉprĚ�

aüąñŎ2017Q12m16iï´Ä²¨100.ŏ13) ķĝįŃōIJĤĸʼnĪō�¢�ïßK��ð��ċ��Ōá1Ō¡ĚÅ�ĆøėgüòDNA�t`º—þċX�ĊĔėÇöĉ��ċ�

Í—ñŎ2018Q2m20iï´Ä²¨20.ŏ14ŏĢİħĭĞ$Æ�¬�ï�ç»+ð2�OIØ� z�¡ĢİħĭĞ �\ćÒ�ċ�Vñ Ŏ2018Q4m8iïx�Nx�·�ÀFċ

yï´Ä²25.ŏ15) 2àz�ċiĝľŋĴðå'ċz�ÀFőz�ć=}Y̱ôĔóőñï��-jðª�&[¡÷ÌĂąòė�*ć¡ċ�@�ñ

Ŏ2018Q5m30iï¦�>AKL AĬŋĮō¹På'Dä^ï´Ä²¨40.ŏ16) prċkĕüh̵Ē�ċ�ÍĩŋĿĪĞłinWd-57ċ·�ćŅĝļīĮĝŇ-ïßK��ð��ċ57YĚ�öüÿÇöĉprĚ

�aüąñŎ2018Q7m21iï´Ä²¨80.ŏ17) ĝŇŁĶ iSchoolĽʼnļğİĩńĶŇŌĞğĻĶōïßK��ðMIG-seq�Ő���ĩōĥŋĨōĚ�òÿ_ÉĉĦĸłŊĝĵ:8Ö��

tñŎ2018Q9m5iï�Ù´¿²¨80.ŏ18) nig³ĽʼnļğİĨōĻĪİĴï�ç»+ðĦĸłZ9ĆOI��Ŏ��=}YŏĚ��ýėñŎ2018Q9m25iïRM�Dåwï´Ä

²500.ŏ19) �q>A�/ AÄT�ÚĬŁĶōïßK��ð��ċ��Ōá1Ō¡ĚÅ�ĆøėgüòDNA�t`ºŐMIG-seq�ċ'�öĕþċ

�¢��ĐĆñŎ2018Q10m24iï´Ä²¨70.ŏ

¥�27

Page 32: V)-ST O1U (P 1UA 5* /3 98 H: ] C E? /30^

\)I���}��R]1) =G)Iaqm=G����ZH8�$B�}{z�}�wv�tZb

u^rc^a�3[��M1[�2017�4,30+Y%�:Y18V�2) y�y���ZBl��5p(QOkqs��2;��ACke^f

F�[�2017�7,30+YForever Green Radio�3) P_`dZ�U2;l+ S�L��v�* D6��J�X �

W'gN9�o 2;h�3"���k[�2018�6,9+�4) -+)IZ���x�~������ <;�3" |��g�s[�2019

�1,9+Y-Y19V�

)I�R��������ji

\�������]1) 0���Z|��#�b

r�?@�/Bv�7�<&EK�T4nl!>k. �[(2019�2,27+)