PRÀCTICA N° 11: OBSERVACIÓN DE ELEMENTOS FORMES DE LA SANGRE

7/23/2019 PRCTICA N 11: OBSERVACIN DE ELEMENTOS FORMES DE LA SANGRE

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UNIVERSIDAD PRIVADA SAN JUAN BAUTISTAFACULTAD DE CIENCIAS DE LA SALUD ESCUELA DE

MEDICINA HUMANA CURSO DE BIOLOGIA CELULAR Y

MOLECULAR

PRCTICA N 11

OBSERVACIN DE ELEMENTOS FORMES DE LASANGRE

I. INTRODUCCIN:

La prese!e pr"#!$#a !ra!are%&s s&'re (&s E(e%e!&s)&r%es *e (a sa+re #,-& &'e!$/& es re#&er - *$)ere#$ar(&s !$p&s *e #0(,(as 1,e se e#,e!ra e (a sa+re.

Vere%&s (&s *$s!$!&s !$p&s *e (e,#$!&s !a(es #&%& (asp(a1,e!as 2e,!r34(&s2 %&$!&s 25e%a!6es 2'as34(&s2($)$!&s2 e!re &!r&s .Para !&*& es!& ,!$($7are%&s e(

#&(&ra!e 8r$+5! 2*e #&(&r /$&(e!a 2#& (a 4a($*a* *e )a#$($!ar(as &'ser/a#$3 *e *$)ere!es #0(,(as sa+,6eas .Es!& (&((e/are%&s a( %$#r&s#&p$& a+re+a*& a#e$!e *e $%ers$3para as6 ,!$($7ar , (e!e *e %a-&r a,%e!& 29; 2- as6p&*e%&s &'ser/ar #& $!$*e7 (a #0(,(as sa+,6eas.

II.OBJETIVOS


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De!er%$ar (a prese#$a *e e(e%e!&s 4+,ra*&s prese!ese ,a %,es!ra *e sa+re per$)0r$#a5,%aa2 %e*$a!e e( ,s& *e ,a#&(&ra#$3 *$)ere#$a(.

O'ser/ar (a prese#$a *e - (&s e(e%e!&s )&r%es2 (a )ase *$spersa *e( s$s!e%a e )&r%a *epe1,e?&s #&rp


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L&s er$!r$!&s2 !a%'$0 ((a%a*&s +(3',(&s r&&s & 5e%a!6es2 s&(&s e(e%e!&s )&r%es#,a!$!a!$/a%e!e%"s ,%er&s&s *e (a sa+re.La 5e%&+(&'$a es ,& *e s,s pr$#$pa(es #&%p&e!es2 - s, &'e!$/& es!rasp&r!ar e( &;6+e& 5a#$a (&s *$)ere!es !e$*&s *e(#,erp&. L&s er$!r$!&s 5,%a&s #are#e *e


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N& prese!a +r",(&s e s, #$!&p(as%a: ($)$!&s2 %&$!&s -%a#r3)a+&s.

LINFOCITOA pesar *e es!as #(as$4#a#$&es - *$)ere#$as e!re (&s (e,#$!&s2 !&*&ssere(a#$&a #& (&s %e#a$s%&s *e)es$/&s *e( &r+a$s%&.L&s +ra,($!&s - (&s %&$!&s *es!r,-e a (&s %$#r&&r+a$s%&s)a+$!"*&(&s %$e!ras 1,e (&s ($)$!&s pr&*,#e a!$#,erp&s #&!rae((&s.


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IV.MATERIALES

Microsco ioColorante

Wri ht o Giemsa Alcohol yodado Agua destilada

Lminas portaobjetoslimpias y Al odn estrilLancetas

hematolgicasVarillas de

Aceite de cedroPapel lenteLminas coloreadasde sangre con Wright

Guantes

Conteiner dedescarte


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V. PROCEDIMIENTO

A. Obtenc!n "e sang#e ca$la#9. Des$)e#!ar e( *e*& a,(ar #& a(#&5&( -&*a*& - a(+&*3 - #& ,a(a#e!a es!0r$( p,7ar e( *e*& - *ear #aer ,a +&!a *e sa+re s&'re ,

e;!re%& *e (a ("%$a p&r!a&'e!&s.. C& a-,*a *e &!ra ("%$a p&r!a&'e!& )&r%ar , "+,(& *e K -rea($7ar , )r&!$s2 !ra!a*& *e espar#$r 5&%&+0ea%e!e (a +&!a *esa+re s&'re (a s,per4#$e *e (a ("%$a.. Dear se#ar (a prepara#$3 - pre*er (,e+& a (a #&(&ra#$3 #& 8r$+5!& G$e%sa.

%. C&lac!n


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9. C,'r$r e( )r&!$s #& #&(&ra!e 8r$+5! & Le$s5%a p&r 9 %$,!&.. S$ e($%$ar e( #&(&ra!e a+re+ar a+,a *es!$(a*a - *ear a#!,ar p&r 9%$,!&s.. E($%$ar (,e+& e( #&(&ra!e - (a/ar s,a/e%e!e #& a+,a *e #a?&.. Dear se#ar (a ("%$a #&(&rea*a..Ua /e7 1,e (a ("%$a #&(&rea*a a se#a*&2 &'ser/ar a( %$#r&s#&p$& #&e( &'e!$/& *e 9 - a#e$!e *e $%ers$3.

VI. PROTOCOLO


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Es1,e%a!$#e (&s s$+,$e!es e(e%e!&s 1,e a'a& se $*$#a - 1,e ser"&'ser/a*&s #& a-,*a *e( %$#r&s#&p$&.

1. LEUCOCITOS 'RANULARES:

a( Neut#!)l&s seg*enta"&s: prese!a ,


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E&sn!)l&s

%as!)l&

Ln,&ct&s

-&n&ct&s


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VII.CONCLUSIONES:


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ANEOS:

CLASIFICACION 2E LOSLEUCOCITOS


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VIII. CUESTIONARIO

1. 3Cu4l es la ",e#enca ent#e $las*a

5 sue#&6.J,!& #& (&s +(3',(&s r&&s2 (&s +(3',(&s'(a#&s - (as p(a1,e!as2 e( p(as%a es,& *e (&s #&%p&e!es *e (a sa+re2 (apar!e (61,$*a 1,e s,p&e , *ee((a.

P&r e( p(as%a #$r#,(a ,a s,s!a#$a((a%a*a 4'r$3+e&2 1,e #,e!a #& (apr&p$e*a* *e #&/er!$rse e 4'r$a#,a*& se pr&*,#e (a r&!,ra *e , /as&

- (as p(a1,e!as a#! ,a /e7 se e($%$a (&s #&a+,(a!es #&%& (a 4'r$a2es!e (61,$*& se #&/$er!e e s,er&.

+.2ga 7ue es la ,!#*ula leuc&cta#a8 cu4les s&n l&s 9alesn*ales en el a"ult& 5 7u sgn)ca"& tene l&s + 9alesan*ales.

La ,!#*ula "e leuc&ct&s& (e,#$!ar$a %$*e e( p&r#e!ae prese!e *e#a*a !$p& *e (e,#$!&s e e( !&!a( *e gl!bul&s blanc&s. A( ser ,

p&r#e!ae a( a,%e!ar , +r,p& *e (e,#$!&s *$s%$,-e &!r&2 a,1,e eas$&es s&(& e;$s!e , a,%e!& & *$s%$,#$3 *e , !$p& #re!& - p&re((& s&(& e( p&r#e!ae &)re#e ,a /a(&ra#$3 &r$e!a!$/a per& se *e'e#&!ar e(


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U /a(&r re#&$*& *e!r& *e( ra+& &r%a( p,e*e ser pa!&(3+$#& e ,s,e!& e par!$#,(ar. Es p&r es!a ra731,e e( !0r%$& /a(&res &r%a(es 5as$*& ree%p(a7a*& p&r e( *e /a(&res *ere)ere#$a.L&s /a(&res *e re)ere#$a pr&/e$e!es*e #5$(e&s res$*e!es e Sa!$a+& *eC5$(e se p,e*e &'ser/ar e e( ap0*$#eI.

.3/u es la


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p,e*e !eer &!r&s s6!&%as2 #&%& )a(!a *e a($e!&2 %are& & *&(&res *e#a'e7a.

La ae%$a +ra/e & pr&(&+a*a p,e*e #a,sar (es$&es e e( #&ra732 e(#ere'r& - &!r&s 3r+a&s *e( #,erp&. La ae%$a %,- +ra/e p,e*e $#(,s&

#a,sar (a %,er!e.

I.REFERENCIAS

La *$)ere#$a e!re s,er& - p(as%a sa+,6e&

5!!p:sa(,*.,#&%&.#&%ar!$#,(,a(es(a*$)ere#$ae!res,er&-p(as%asa+,$e&[email protected]!%(5!!p:QQQ.*$)ere#$ae!re.e!(a*$)ere#$ae!rep(as%a-s,er& 5!!p:%e*$#a'(&+s.*$ar$&%e*$#&.#&%$*ee%$p9

Us&s *e (a 5$s!a%$a5!!p:QQQ.e5&Qeespa&(.#&%,s&s5$s!a%$a($s!a5!!p:a(er+$a-as%a.es1,ees(a5$s!a%$a-1,e$%p&r!a#$a!$eee(asrea##$&esa(er+$#as 5!!p:%e*%&(.es+(&sar$&

Me*$a*&res *e %as!$!&s2 a##$&es - !ra!a%$e!&5!!p:#a$'#&.,#/./e#a$'#&/$!aeV$!aeC$#&Ar!$#,(&sPe*$a!r$aMe*$a*&r.5!%

La Ae%$a5!!p:QQQ.5('$.$5.+&/5ea(!5spa$s55ea(!5!&p$#s!e%asae%$a5!!p:QQQ.eQs%e*$#a(.e!5ea(!5S$#(eCe((D$seaseH$s!&r-Spa$s5.asp;5!!p:QQQ.Qe'#&s,(!as.#&%#a!e+&r$asa(,*a(*$aae%$a

La sa+re5!!ps:es.s#r$'*.#&%*cO'ser/a#$&*eE(e%e!&sF&r%es*eLaSa+re5!!p:QQQ.',eas!areas.#&%esa-&sE(e%e!&sF&r%esDeLaSa+re.5!%(
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Ne,!r34(&s e 'a*a

5!!p:(e,#$!&s.&r+e,!r&4(&s'a*a5!!p:aa($s$s*esa+re.&r+e,!r&4(&s'a*a

He%a!6es5!!p:QQQ.aea(.es$*e;.p5p(asa+re-(a%e*,(a&sea&r%a(es(as#e(,(as*e(asa+re$1,es&(&s+(&',(&sr&&s5e%a!$es&er$!r$!&s5!!p:#$e!$4#&se),re#$*&s.'(&+sp&!.#&%9@1,ees(asa+re.5!%(

T$p&s *e (e,#$!&s5!!p:+r$5&.,*(.es#ar(es%e*$#$asp(e,#&s.5!%(

E&s$34(&s

5!!p:(e,#$!&s.&r+e&s$&4(&s

5!!ps:es.Q$$pe*$a.&r+Q$$E&s$CB4(&5!!p:+r$5&.,*(.es#ar(es%e*$#$aspe&s$.5!%(

M&$!&s5!!p:+r$5&.,*(.es#ar(es%e*$#$asp%&&.5!%(5!!ps:es.Q$$pe*$a.&r+Q$$M&$!&5!!p:(e,#$!&s.&r+%&$!&s

L$)$!&s

5!!p:-asa(,*.#&%($)$!&s

5!!ps:es.Q$$pe*$a.&r+Q$$L$)$!&5!!ps:es.Q$$pe*$a.&r+Q$$L$)$!&

'as34(&s5!!ps:es.Q$$pe*$a.&r+Q$$BasCB4(&5!!p:(e,#$!&s.&r+'as&4(&s

Ne,!r34(&s

5!!p:(e,#$!&s.&r+e,!r&4(&s5!!ps:es.Q$$pe*$a.&r+Q$$Ne,!rCB4(&

P(a1,e!as5!!p:QQQ.e#,re*.#,$*e;.p5pC&!e&*ep(a1,e!as 5!!p:QQQ.#a#er.+&/C&%%&P&pUpsp&pDe4$!$&.asp;$*W/ers$&Pa!$e!W(a+,a+eSpa$s5
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PRÀCTICA N° 11: OBSERVACIÓN DE ELEMENTOS FORMES DE LA SANGRE

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