Slide 1

Autores / Authors: C. Soriano 3 ; C. Gil-Gas 1 ; P. Castro-Garca 1 ; P. Honrubia 1 ; C.B. lvarez-Simn 1 ; J. Ferre 1 ; S. Sabater 1,3 ; G. Aparicio 2 ; L. A. Aparicio; Misco Jones 1 y C. Ramrez-Castillejo 1. Filiacin / Hospital: (1) Laboratorio de Biologa Celular y Molecular de la Clula Madre. Centro Regional de Investigaciones Biomdicas / Facultad de Medicina. Universidad de Castilla La Mancha. (2) Oncology Research Unit. CHU A Corua, Spain. (3) Complejo Hospitalario Universitario de Albacete. (CHUA). Introduccin y objetivos: Objetives: The cancer stem cell hypothesis suggests that cancer contains cancer cells with stem characteristics. (Reya et al, 2001). Several publications have described the cancer stem cell population in cell lines, for instance in the C6 glioma cell line (Kondo et al, 2004). Our work consist of analyzing the characteristics of this small cancer stem cell population in the C6 glioma. We have found a small population defined by the expression of two biomarkers that have previously reported in both stem and cancer stem cells. We have found 0,6% BCRP1+ cells, which Kondo et al. reported as the principal protein responsible for the Side Population in the same cell line, counting for similar values as we described in this report. BCRP1+ protein is related with the bone marrow stem cell population and confers resistance against toxics. On the other hand, we have found a smaller CD133+ population than the previous one. CD133 is a self-renewal related protein. Moreover, the whole CD133+ population match with a part of the BCRP1+ cells (BCRP1+ AC133+), suggesting that CD133+ cells could be a subpopulation of the BCRP1+ cells, and therefore, a more specific cancer stem cell population than previously reported in the C6 glioma cell line. Furthermore, these CD133+BCRP1+ cells display an aggressive phenotype when injected in NOD/SCID mice. Eventually, we have also found the cancer stem cell population (BCRP1+ CD133+) in glioma patients with aggressive disease, but not in patients with good prognosis. A Cancer Stem Cell population in the C6 glioma cell line. (1) Reya et al, Nature 2001 (2) Kondo et al, PNAS, 2004 Referencias: References: Mtodos: Methods: Resultados: Results: AC133 AND BCRP1 POSITIVES CELLS STUDY IN XENOGRAFT SCID MOUSE MODEL. (A) Sorted cells in the C6 glioma line (Left) AC133 + /BCRP1 -+ population (B) Positives and negatives cells were injectedintraperitoneal into nude mice (4000 cells per inject), four weeks after the injection, mice were photographed and killed. Tumor sizes were measured and tumor growth volume were obtained (Right) the bars indicate differences between AC133 + /BCRP1 -+ and AC133 - /BCRP1 - tumors (C) Tumors formed were fixed and photographed by confocal microscopy. Histology was verified by hematoxiline stain. The graphic shows the significative difference (p